腸管出血性大腸菌 O157を用いたパルスフィールドゲル 電気泳動
Contents
1. 0157 PCR IS printing system 0157 IS PCR PFGE IS printing sys tem 1 0157 18 61 1 1 4mL LB Broth DIFCO2. PFGE 11 13 14 15 Z AC 12 17 18 AE 1S printing system 11 14 12 17 3 15 IS printing system PEGE 0157H7 s 1 VCLA4OET A I 010 I I I 0 0 I 0 0 I I I 0 I T I I II1100 I 1 I I 0 0 I 0 I I 19 ST VcGAITET A I I 010 I I I I 0 0 I 0 0 I 0 I 0 I t P OD E TOE O T E PO E 0 000 0 OPE 99 LE V2 LAAEA A T I 010 I I I I 0 0 I 0 0 I I I 0 T II L T LT TOL TLTIT ITL OO OCL TI G9 9 dc 4ATET 4 I I I 0 TIT I I I 0 0 I 0 0 I I I 0 I I I I I I I OO IT I I I 0 0 I 0 I I 79 ST qeddIET A I I 0 I I I I 0 0 I 0 0 I 0 I 0 I F CE CPE T P 0 T E T TP 030 TOT 1 69 FI Ve G q6 1 J I 0 0 I I I 0 0 0 I 0 0 I 0 I I I I I 0 I I 0 0 I 0 I 0 0 I I I 0
3. IS printing system 1 2r IS 16 4 4 16 L 1 o F 2 9 16 10 18 20 x 1
4. 50 378 380 2006 2 24 74 79 2007 3 61 127 2006 4 41 29 31 2008 5 27 74 76 2008
5. Rep Hokkaido Inst Pub Health 59 67 71 2009 0157 PFGE 1IS printing system Comparison of Pulsed field Gel Electrophoresis PFGE and IS printing System for Analysis of Escherichia coli O157 aA Shunichi SHIMIZU Yo MoRrMoTo Rika KOMAGOME and Keiji YAMAGUCHI Key words IS printing system IS Pulsed field Gel Electrophoresis PFGE Escherichia coli 0157 0157 2 0157 lt PFGE PFGE PFGE
6. 6 F 10929904 7 00929904 9 F F1DEF23B 10 F C29B7232 11 14 F D3DF527B 12 17 F D3DF527A 13 F 729BD23A 16 F D3DF727A 18 F D3CF727A 15 F D3DF727B 3 2 IS printing system PFGE 8 PFGE IS printing system PFGE AK 5 6 7 IS printing system IS printing system tz 6 2 hlyA IP 1 04 2
7. 37 C 2 PFGE 1mL 1 5mL 100 gL 30 TE buffer 150 gL TE buffer CHAL r Proteinase K Xba I CHEF DR II BIO RAD 6 V cm 2 2 54 2sec T 0 5XTBE buffer 14C 21 PFGE Fingerprinting II BIO RAD 3 IS printing system 500 uL 1 5 mL v IS printing system Ver 2 TOYOBO PCR 1 0 IS printing system hlyA eae St
8. 1996 45 O157 H7 Stx1 Stx2 Patient MUG 46 O157 H7 Stx1 Stx2 Carrier MUG 7 1996 47 0157 H7 Stx1 Stx2 Patient MUG 48 50 0157 H7 Stx1 Stx2 Carrier MUG 8 1998 51 53 0157 H7 Stx1 Stx2 Patient 9 2007 57 0157 H7 Stx1 Stx2 Patient 10 2007 58 0157 H7 Stx1 Stx2 Patient 11 2007 59 0157 H7 Stx1 Stx2 Carrier 12 2007 60 0157 H7 Stx1 Stx2 Carrier 13 2007 61 0157 H7 Stx1 Stx2 Patient 14 2007 63 0157 H7 Stx1 Stx2 Patient 15 2007 64 0157 H7 Stx1 Stx2 Patient 16 2007 65 0157 H7 Stx1 Stx2 Patient 17 2007 66 0157 H7 Stx1 Stx2 Patient 18 2007 67 0157 H7 Stx1 Stx2 Patient 4 AI 5 6 7 AK 3 1 2 3 4 8 AH 11 13 14 15 AC 12 17 18 AE 9 AF 10 AB 16 AG 1 IS printing system 16 1 D C69BD23A 2 F F35F783B 3 F D3DF727B X F 19CB4282 5 7 10884000 7 F 93529904 8 F 93CB447B
9. 9 cI VcGATET A I I 010 I I I 0 0 I 0 0 I 0 I 0 I Leo EPE TOO E OO E 0221 I 09 cI aesded I I 0 I I I I 0 0 I 0 0 I 0 I 0 I F D e p PE E O POE pO PO 0200 100 Ob T 6S II cec4d6 O A I I 0 0 0 I 100 1 0 I 0 0 I I I 0 I I I I 0 I I 0 0 I 0 I 0 0 0 0 I I 8S OT aEcAAAIAH A I I T 0 I I I 0 0 0 0 0 I I I I I 0 I II1II0H TI I I 0 0 0 I I I I LS 6 gd yFdOS6 1 I I LI 0 I I I 0 0 0 I 0 0 0 I 0 I IT I I 0 0 0 1 1 I I I 0 0 I 0 0 I SI 8 0662G66 J I I 0010 0 0 0 0 I 0 0 I I 0 0 TI I 0 I I 0 0 1 0 I 0 I I 0 0 I 0 0 0 L 0662600 I I 0 0 I 0 0 0 0 0 I 0 0 I I 0 0 TI 0 0 I I 0 0 I 0 0 I 0 0 0 0 0 0 0 0 9 0666014 I I 0010 0 0 0 0 I 0 0 I I 0 0 l I 0 T I 0 0 0 0 I 0 0 0 0 I 0 0 0 sr I 0008S80 I I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 T 0 0 I 0 0 0 I 0 0 0 0 I 0 0 0 WU S 9 ydO61I 4 I I 0100 0 0 0 I 0 I 0 0 0 0 I 0 I I I I 0 I 0 0 I I I 0 0 I I 0 0 0 6 8 8 f dc 4ATET 4 I F 0 EE EO OO Pa 0 I POE Bob ET 0 E EE TO0 0 ECO 0 tE E ENTE dc9 4Se1 4 I LI 0 I I I 0 0 0 0 0 I I I I O EP Eor po F Os O E F 030070 Loe L eE E OUR 2 Vedd69 O 0 I 0 0 I I I 0 0 0 I 0 0 I 0 I I I I I 0 I I 0 0 I 0 I I 0 0 0 I I S l I IXJS XS 9 SL FI I I II OL 6 8 9 S f I VAIH 9 L GT PI EL ZI IT OI 6 8 42 9 G f Z I i4 Sm 19S puz 19S ST i E o 91SAS 8uiuud SI z 3 PFGE IS IS PFGE 1 125 D C69BD23A AA 2 6 10 F F35F783B AJ 3 11 36 F D3DF7
10. 27B AD 4 38 39 F 19CB4282 Al 5 41 42 7 10884000 AK 6 45 F 10929904 AK 46 7 00929904 AK 7 47 50 F 93529904 AK 8 51 53 F 93CB447B AH 9 57 F F1DEF23B AF 10 58 F C29B7232 AB 11 59 F D3DF527B AC 12 60 F D3DF527A AE 13 61 F 729BD23A AC 14 63 F D3DF527B AC 15 64 F D3DF727B AC 16 65 F D3DF727A AG 17 66 F D3DF527A AE 18 67 F D3CF727A AE IS printing system 1 18 2nd 18 36 1 0 SE IS printing system S 16 0 9 AF
11. xl Stx2 st O 1 46 2n8 1 16 4 16 1 1 IS 4 2 9 4 PFGE Dice UPGMA 1 0 90 0 100 0 AA AK 11 1 Z AA 2 AJ 3 AD 1 1 2007 1 5 O157 H7 Stx2 Patient 2 2006 6 10 0157 H7 Stx1 Stx2 Patient 11 18 0157 H7 Stx1 Stx2 Patient 19 20 0157 H7 Stx1 Stx2 Staff 3 2005 21 30 O157 H7 Stx1 Stx2 Patient 31 32 0157 H7 Stx1 Stx2 Carrier 93939 0157 H7 Stxl Stx2 Staff 36 0157 H7 Stx1 Stx2 Relatives 4 1996 38 39 0157 H7 Stx1 Stx2 Patient 5 1996 41 43 O157 H7 Stx1 Stx2 Patient MUG 44 0157 3 H7 Stx1 Stx2 Swab MUG
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