分解から眺める生命現象 - Thermo Fisher Scientific
Contents
1. Total Exosome Isolation2. 20 30
3. Expi293 Expression System 8 C0 FreeStyle WM 2 10 ER Mi Expi293T Expression System Kit 1
4. 2006 100
5. YA6 2013 SERNDA WDR45 Altg18 WIPI4
6. 3 12 EV0S FL Auto
7. GeneArt Genomic Cleavadge Detection Kit ZFN TALENs CRISPR IA LE
8. Deep Red Green CellMask
9. RNAi PCR TALENs CRISPR Applied Biosystems page 16 2720 10 SimpliAmpT 96 well 0 2mL 8 p
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12. 005D 0 CU GFP LC3 24
13. EdU DNA EdU Technical Review Click iT Plus EdU Alexa FLuor 488 Flow Cytometry Assay Kit NEW C10632 Click iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit Classic C10425 EdU Jurkat EdU final10uM 37C5 C0 90 PE CD3 MHCD0304 FxCycle Violet Stain F10347 Attune Acoustic Focusing Cytometer Violet Blue laser type Click iT Plus EdU New amp Click iT EdU Classic Time EdU 1 2hour 5min
14. 1 page 15 1 PCR pp www lifetechnologies com GenomeEditors GeneArt Genomic Cleavage Detection Kit
15. OVO OO OOO OO OO OOO OO Since 1981 Applied Biosystems 1987 PCR 27 PCR SimpliAmp 2720 10 Applied 9 applied Biosystems biosystems ProFlex PCR _ _ partof ecnoogeer 1981 Applied Biosystems GeneAmp PCR 2013 0 F 1987 Invitrogen Corp Veriti 2 GeneAmp S PCR OO i _Sooooooooooooooo
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17. Em Click iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit 50 1 C10632 85 000 Click iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit 50 1 C10634 85 000 Click iT Plus EdU Pacific Blue Flow Cytometry Assay Kit 50 1 C10636 85 000 CD3 Mouse Anti Human mAb PE conjugate 0 5 ml MHCD0304 37 UUU FxCycler Violet Stain 1 F10347 60 000 em Probes page 10 Antibody Conjugate Purification kits A Molecular Probes amp 5 20 50ug 15 50 100pug 0 5 1mg 10 20 50ug 45 50 100ug 0 5 1mg Alexa Fluor HRP
18. mRNA 2 CRISPR Cas Cas mRNA guide RNA CW DNA PCR TALENs CRISPR Cas9 DNA amp n GeneArt Genomic Cleavage Detection Kit 20 A24372 42 000 Cell lysis bufferAmplTad Gold 360 Master Mix Cotrol Template amp Primers Protein Degrader10XDetection Reaction BufferDetection Enzyme Water GeneArt Genomic Cl
19. RNAi 9
20. RNA 1 www lifetechnologies com exosome jDp page 14 TAL TALENs CRISPR Cas9 TALENs CRISPR Cas9 ro mRNA Ambion mMESSAGE mMACHINE Kit TALENs CRISPR Cas9 mRNA 80 in vitro RNA
21. 10 5 cells ml 10 000events 1 4 1 14 Click iT Plus EdU Jurkat Click iT Plus EdU Click iT EdU DNA
22. 0 5 1um 1 9
23. EVOS FL Auto iPS EVO0S FL Auto
24. URL www lifetechnologies com nextforum QU0 photographs muraguchi keiichiro p 02 03 art direction amp design masami furuta opportune design Facebook amp Twitter f facebook com LifeTechnologiesJapan QLifetechJPN 2014 6 www lifetechnologies com TC For Research Use Only Not for use in diagnostic procedures 2014 hermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specl
25. RNase SUPERase InTWM DNase EE mMESSAGE mMACHINEYT7 ULTRA Kit 10 AM1345 82 000 mMESSAGE mMACHINE T7 Kit 25 AM1344 82 000 mMESSAGE mMACHINE T3 Kit 25 AM1348 82 000 mMESSAGE mMACHINE SP6 Kit 25 AM1340 82 000 Poly A Tailing Kit 25 AM1350 33 500 1T7 ULTRA Kit AM1345 Poly A Tailing Kit AM1350 mRNA ya VP64
26. 1 9 EVOSY FL Auto EVO0S EV0S FL Auto EVO0S FL Auto
27. 5 10 mRNA Ambion DmMESSAGE mMACHINE Kit A Poly A Tailing Kit RNA MEGAclearrM Kit TALE Activator TAL
28. 24 5 30 30 35 30 199 1 1996 2006 2012 2008
29. en mm Tim i Fn lar Fee ie FLF www ampliseq com page 19 Technologies gt Name _ re Number 07 C e e9a7s
30. 2004 EGFR PCR lon PGM DNA 10ng DNA 1 DNA 30 100ng 10ng lon PGM lon AmpliSeq
31. _ 405nm 488nm 515nm RA RE 2 8 9 ee 340 40O 440 ns PT TE Di roGFP 0r1 Frx 1 H 0 0rp1 roGFP HN EE i 9 BO BacMam technology roGFP a 1 Premo Cellular hydrogen peroxide H 0 Sensor 2 Premo Cellular hydrogen peroxide 0rp 1 H 0 H 0 Hz0 Sensor H 0 0rp1 H 0 roGFP Premo Cellular hydrogen Premo Cellular Hydrogen Peroxide H 0 Sensor peroxide H 0 Sensor
32. C e egans KOD gl 1992 1992 1996 1996 200 1 2010 4 INFORMATION 2014 June No 25 6 8 Miraikan 20 DNA
33. SENDA 1963 1980
34. scFv SA SA scFv SA SA SA Expi293 Expression System E
35. 2013 nh page 04 RN_WORKFEOVW_E8SY SeDarate WES TE Boltr amp STEP 2 gt gt Transfer iBlot 2 STEP 3 gt gt Detect iBind Western System page 05 4 Fast Clear pH Bolt 16 2
36. Applied Biosystems k PCR essentials WiFi www lifetechnologies simplamp Messagqes from PCR C AmpliTaq Platinum Taq PCR SuperScript Big Dye our Technicol Support Sko pp Gan A
37. BoltTY amp ES Bolt Welcome Pack A 4 12 10well B0412A 68 000 Bolt Welcome Pack B 4 12 15well B0412B 68 000 Bolt Welcome Pack iBlot 2 1 B0412AIB2 248 000 BoltT Bolt 7 7 iBlot 2 iBlot 2 Gel Transfer Device 1 IB21001 198 000 iBlot 2 Transfer Stacks nitrocellulose Tedular size 10 IB23001 22 100 iBlot 2 Transfer Stacks nitrocellulose
38. 56 EVOS FL Auto Z Stack 96 384 XY Light Cube EVOS FL Auto System 4XPh 10XFL 20XFL 40XFI GFP RFP DAFI amp 6 800 000 EVO0S 5 www lifetechnologies com evos er Probes page 12 Premo Cellular Redox Sensor Premo Cellular hydrogen peroxide H 0 Sensor A
39. FISH RNA 1 EML4 ALK lon PGM IM lon ChefrW DNA Cell free DNA cfDNA lon AmpliSeq ww EN BRCA1 2 Colon amp Lung TP53 CFTR Dimentia Human ldentity pp 10ng DNA FFPE 500 CNV
40. PE CD3 CD3 4 Click EE HR tl ei ms ea Fr 3 EdU Alexa Fluor 488azide 4 PE CD3 iT Plus EdU Click PE Click iT Plus EdU Click iT EdU Click iT Plus EdU Click iT EdU Click Click No Azide No Azide Click iT Plus PE Click iT Plus EdU Click iT EdU Click iT Plus EdU DI ANG OT A COA FIO 0222 TE Click iT Plus EdU PE PE GFP Pacific BLue GFP PE
41. En i CellMask Green plasma membrane stain 100 pl C37608 24 000 CellMask Orange plasma membrane stain 100 pul C10045 24 000 CellMask Deep Red plasma membrane stain 100 pl C10046 24 000 page 11 ee Technologies EV0S FL Auto
42. 1 2 Attune Acoustic Focusing Cytometer Click iT PlLus GFP PE S BrdU DNA BrdU DNA EdU BrdU EdU Brd U Anti Brdu antibody Ed U BrdU
43. Cancer Hotspot Comprehensive Cancer Colon amp Lung Cancer Ion AmpliSegrM Community 2014 4 AACR 0ncoNetwork lon AmpliSeq RNA lon AmplisSeq RNA Lung Fusion PaneU 4K ROS7 RE7
44. 15 30min Click iT Fixative 15min Click iT permeabilization buffer 15min Click iT 30min 15 30min 15 30min Click iT page 09 ee Technologies 0FF ON i i Mi Attune NxT Acoustic Focusing Cytometer Blue Laser A24864 Attune NxT Acoustic Focusing Cytometer Blue Red Lasers A24
45. 9600 9700 2720 7600 9700 29600 9700 VeriFlex 3 Zones VeriFlex 6 Zones VeriFlex 6 Zones VeriFlex ND 4 0C 5 0C 5 0C 6 0C 460 000 628 000 850 000 980 000 1 250 000 page 18 PD lon PGMT AmpliSeq DNA lon PBMW lon AmpliSeq lon PGM 1 400 500 EGFR
46. AS PI Joel Rothman a 3 Joel Rothman 2 2011 6 UCLA International C elegans Meeting 6 2N M 1990 RNAi C 50lgW6
47. 10 2013 3 TALENs TALENs mRNA F1
48. 30 AHA C10102 0PP C10459 AHA 0PP Click iT Alexa Fluor 488 Alkyne A10267 Alexa FLuor 647picolyl azide C10456 712200 Alexa Fluor 555 A21429 A Click iT AHA Alexa Fluor 488 Azide protein synthesis B C Click iT 0PP Alexa Fluor 647 picolyl azide D in vivo 0PP 3 Tetramethylrhodamine TAMRA Azide T 10182 Click iT Quant iTTV OlLiGreen ssDNA Reagent 07582 Basal Secti
49. A14635 197 000 ExpiFectamine 293 Transfection Kit 1 A14524 92 000 Expi293T Expression Medium 1000 ml A1435101 26 000 _Expi293F Cells 1 ml A14527 55 000 Antibody Expressing Positive Control Vector 1 150ug A14662 58 000 Opti MEM 1 Reduced Serum Medium 100 ml 31985062 1 600 page 07 Molecular Probes Click iT Plus OPP Alexa Fluor Protein Synthesis Assay Kits A 0 in vivo in yro 1 Liu J et al 2012 Proc Natl Acad Sci U S A 109 413 418 Click iT Plus 0PP 2 J kn Click 8 a 0PP C HeLa
50. ReadyProbes Cell Viability Imaging Kit Blue Green 1 R37609 28 000 ReadyProbes CellViability Imaging Kit Blue Red 1 R37610 28 000 page 13 mm Leading Edge Topic DDS DDS 0 201 1 JST ERATO RNA
51. H 0 Premo Cellular Redox Sensor Grx 1 roGFP HeLa 25buM 15 roGFP Sn Mi 3 PremorM Cellular Redox Sensor Grx 1 roGFP 5 ml P36242 73 000 Premo Cellular hydrogen peroxide H 0 Sensor Orp1 roGFP 5 ml P36243 73 000 ReadyProbes Cell Viability Imaging Kit OK Rea WT 23 XSF NE rd
52. R phycoerythrin 8 kDa SH Mi Antibody Conjugate Purification Kit for 0 5 1 mg 3 1 A33086 27 000 Antibody Conjugate Purification Kit for 50 100 ug 5 1 A33088 27 000 Antibody Conjugate Purification Kit for 20 50 ug 3 1 A33087 27 000 Alexa Fluor 488 carboxylic acid 5 mg A33077 41 000 Alexa Fluor 546 carboxylic acid 5 mg A33079 41 000 Alexa Fluor 555 carboxylic acid 5 mg A33080 41 000 Alexa Fluor 647 carboxylic acid 5 mg A33084 41 000 Molecular Probes Alexa Pasific Blue www lifetechnologies com LCST CellMask Green Plasma Membrane Stain A
53. p 0 6 p 15 AppliedBiosystems AS SimpliAmp II 2 9600 MEGaWSk E M9 PCR 0eeslsaliDiG lm SimpliAmp 628 000 2 668 000 page 17 meeeeewsme Biosystems S 8
54. ifeteehnologies com amazingsciencee 2014 7 31 100 T PDF www lifetechnologies com GenomeEditors NEXT 6 9 aka4 EE GW 0E 5H INIUoPAEl440101IEP 2 PAC0 4
55. 863 _ Attune NxT Acoustic Focusing Cytometer Blue Violet Lasers A24862 Attune NxT Acoustic Focusing Cytometer Blue Yellow Lasers A24861 Attune NxT Acoustic Focusing Cytometer Blue Red Violet Lasers A24860 Attune NxT Acoustic Focusing Cytometer Blue Violet Yellow Lasers A24859 _ Attune NxT Acoustic Focusing Cytometer Blue Red Violet Yellow Lasers A24858 M Jurkat EdU rc PE CD3 Gc Click iT Fixative Click ee IT permeabilization buffer EdU FxCycle M Violet Stain DNA Attune Acoustic aa Focusing Cytometer Violet Blue laser type PH TI 1 RR 1 EdU FxCycle Violet 2 TM lt rxCycle Violet S
56. eavage Detection Kit TALENs CRISPR ZFN TALENs Platinum TALEN CRISPR GeneArt Genomic Cleavage Detection Kit
57. fied Printed in Japan LFT063 A14050B 108 0023 4 2 8 TEL 03 6832 9300 FAX 03 6832 9580 564 0052 10 28 TEL 06 6389 1201 FAX 06 6389 1206 www lifetechnologies com technologies AThermo Fisher Scientific Brand
58. ion kit TS page 06 RI Expi293TM Expression System m VR scFv FIRST FIRST scFv
59. mini size 10 IB23002 17 400 iBlot 2 Transfer Stacks PVDF regular size 10 IB24001 24 2UU iBlot 2 Transfer Stacks PVDF mini size 10 IB24002 19 000 2 2 5 S N iBind Western System EE iBind Western Starter Kit 1 SLF1000S 168 000 iBind Western Device 1 SLF1000 158 000 iBind Cards 10 SLF1010 17 000 iBind Solution Kit 1 10 SLF1020 8 000 iBindTMDevice iBindTM Card iBIndTM Solut
60. on EE Click iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit 1 C10456 90 000 Click iT Plus OPP Alexa Fluor 594 Protein Synthesis Assay Kit 1 C10457 90 000 Click iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit 1 C10458 90 000 OPP O propargyL puromycin 9 md C10459 180 000 OPP 2 20uM 1 25uM eemen Technologies page 08 Attune NxT Acoustic Focusing Cytometer A
61. oooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooo 9700 9 Wf SimpliAmp Invitrogen Plutinum Taq 2720 Applied Biosystems AmpliTaq Gold 2014 2004 1987 1980 1990 2000 2010 A _Coooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooeoooooooooooooooooooooooooooooooooooooooooooooooooo J 2720 SimpliAmp GenAmp 9700 Veriti ProFlex PCR PCR PCR a A a 60 0 5mU 3xX32 0 2mD 60 0 5mD 96 2X96 0 2mD 96 2X96 0 2mD 96 0 2mD 96 0 2mD 2X384 MM 2X384 2 Flat 2xFlat 2
62. tain DNA x DNA DNA DNA 2 G1 GO S G2 M 2N 4N DNA 2 1 Click FxCycle Violet Stain DNA FxCycle Violet Stain 2 Click iT Plus EdU DNA Click iT EdU IT Plus EdU Click iT EdU 2 EdU EdU Alexa FLuor 488azide 3 Click iT Plus EdU 46 Click iT EdU 36 DNA S 36 46
63. technologies NEXT 6 2014 June No 25 6 NEXT Interview Pp o4 Boltm amp iBlot 2 iBind Western System P 06 Expi293 Expression System a p 07 Click iT Plus OPP Alexa FLuor Protein Synthesis Assay Kits 1 Pos Attune NxT Acoustic Focusing Cytometer P10 Antibody Conjugate Purification kits CellMask Y Green Plasma Membrane Stain SM Pp 11 EVOS FL Auto P 12 Premo Cellular Redox Sensor Premo Cellular hydrogen peroxide H202 Sensor ReadyProbes Cell Viability Imaging Kit pm14 Ambion mMESSAGE mMACHINE Kit GeneArt Genomic Cleavage Detection Kit P 16 SimpliAmp 96 well 0 2mL P 18 lon AmpliSeq Community NTs A page 02
64. xpi293 Expression System Expi293 FreeStyle W 30 30 ml Expi293 Y Expression System 10mg 200ml GeneArt
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