Biotechnology Explorer - Bio-Rad
Contents
1. 58 C STR RMP 1 STR 2 STR http www cstl nist gov biotech strbase2. 13 110 1 Wo E
3. 56 DNA 1 lt CODIS gt CODIS 13
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6. 2 DNA PCR PCR 3 DNA PCR DNA DNA 2 DNA PCR 4
7. DNA DNA PCR DNA STRs DNA 1 8 HEX4
8. DNA 5 30 ED Eie DH EU DNA Short tandem repeats STRs STRs 2 7bp base pair FO A G S O72 OD DNA Fig 1 DNA 1 TH01
9. zn 1 TV E 1 3 A DE DNA ik f DNA DNA
10. E 55 BE BS Lo BAR 0 H 0 j 2 ED DNA DNA e 1 E
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12. 73 1 lf MME TF ORM ASHRERBU CIS BEGS XU C V DEK LIE FB CC 8152500 H 1 Benecke M DNA typing in forensic medicine and in criminal investigations a current survey Naturwissenschaften 84 181 188 1997 2 Butler JM Forensic DNA Typing Academic Press San Diego 2001 3 Butler JM et al Allele frequencies for 15 autosomal STR loci on U S Caucasian African American and Hispanic populations J Forensic Sci 48 908 911 2003 4 Byrd M DNA The Next Generation Technology is Here http www crime scene investigator net DNA html 5 Crime Scene Investigation gateway site to information about crime scene investigation education jobs and more http www crime scene investigator net 6 DNA Typing and Identification http faculty ncwc edu toconnor 425 425lect15 htm 7 ENFSI DNA WG STR Population Database www strbase org 8 Leclair B et al Kinship analysis and human identification in mass disasters the use of MDKAP for the World Trade Center tragedy Genetic Identity Conference Proceedings Thirteenth International Symposium on Human Identificati
13. DNA 2 281 DNA 2 RAED 3 DNA DNA DNA DNA
14. 1 7 Fig 12 Crime Scene Investigator PCR Basic THO1 37 iN Lesson 2 1 DNA 2 DNA H 2 cR T E 3 DNA 4 DNA 38 d E Lesson 2 gt E fi 1 a 3 Lesson 1 PCR DNA A DNA B DNA C DNA D DNA 1 TAE
15. 15 seen s Fig 12 2 5 2 5 2 amp 41 1 TH01 2 DNA 3 A DNA 4 B DNA 5 C DNA 6 D DNA Lesson 3 gt 1 PCR 2
16. 6 2 4 1 ME 2 RMP
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20. Caucasians African Americans Hispanics 62 BA 1 Qc 2 RMP RMP 3 RMP
21. lt gt DNA 3730 PCR DNA DNA DNA DNA 428 DNA DNA 1 DNA DNA A gt PCR
22. DNA ug Fig 21 STR 7 4 54 STRbase www strbase org E C DNA
23. E 4 STR ee VWA 12 10724 D8 isk 8 8 19 D5 8 5 77 16 THO1 11 3 14 8 1 2 OF 1 4 VWA 13 1 4 18 3 1 4 16 14 2 0
24. 13 Fast Blast DNA lt Fast Blast DNA 10 gt Fast Blast Fast Blast 10 1 1 Fast Blast ml Fast Blast Zt 2 Fast Blast 441 1 1 3 FV E
25. DNA 13 STR CODIS 13 1 1 65 CODIS 2 59 4 STR 1
26. DNA 100 Ha E ES K tH IK am I 52 74 A B C D 5 2 7 2 10 3 Et bp id DNA 771 980 PCR 8 DNA PCR
27. PCR 3 DNA PCR 4 DNA
28. ZH x 99 9940 DNA fh 23 DNA RNA mRNA mRNA 25 000 3 4 E 1 7 FEND 3 DNA 23 46 ED E
29. PCR zi 21 PCR PCR 3 DNA 94 30 52 30 72 1 DNA fig 7 PCR 35
30. DNA zl LE Co gt DNA icit 100
31. DNA 2 DNA 2 DNA PCR PCR DNA 3 DNA
32. PCR MMA ROO BR DNA gt A B 3 AS By pA A ABER THO 12 1 3 1 TCAT 6 Fig 2 x eye DNA B THOI DNA 6 3 gt 1
33. 10 gt 2 1 170 2984 ELET 2 3 2 2 2 3
34. DNA NE DNA DNA gt Fast Blast DNA
35. 2 gt Fast Blast DNA DNA 1 Fig 10 29 Fig 12 37 PCR
36. DNA DNA DNA DNA PCR DNA gt 1 DNA 1 DNA PCR PCR DNA
37. RAT OPES O 7 by A BE DNA DNA E H 4 T BL LZ VERS lt Se H
38. E Lesson Lesson 1 PCR eAE til gt D gt Ga ue REDI dTTP dCTP dGTP 7377 Taq DNA PCR
39. Fig 22 13 CODIS Core STR Loci with Chromosomal Positions 19 44 16 16 17 18 19 20 21 22 X Y Fig 22 CODIS STR 13 7 STR 13 PCR X Y PCR DNA 13
40. 10 5g 50 TAE 60 ml 3L 500 ml 2 1 IL 1 50 ml 1 60 1 4 8 8 1 4 8 AFIT 1 10 TAE 50 1 275 300ml IX TAE 8 8 1XTAE 3L 50xTAE 1xTAE 3L 2 94L 50xTAE 60ml
41. 1 DNA DNA ILesson 1 1 PCR DNA PCR DNA
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43. PCR DNA MMP d PCR MMP 5 PCR DNA PCR 6 36 Lesson 2 PCR PCR PCR e
44. PCR 166 2600JEDU 20 20C Crime Scene DNA 250uL A DNA 230uL B DNA 250uL O DNA 250uL D DNA 230uL 1 2 ml 2x Crime Scene Investigator 25uL S0x Crime Scene Investigator 200nL G 1 ml Sx PCR 0 2 ml 1 PCR 15 ml 1 2 0 ml 1 166 2605JEDU 23g 1 50xTAE 100ml 1 Fast Blast DNA 100ml 1 20 200pL
45. 94C DNA Y AA M N 52 Taa 177 Y 2 oO Taq Q 72C l DNA EO FETE EEE E CO 35 Fig 16 POR 33 2 DNA 35 VAY VOR HAY DNA 3 4X10 PCR DNA 2 DOR 2 DNA DNA
46. B C STR gt DNA Fig 3 2 A B d E STR STR
47. Polymerase chain reaction PCR 35 DNA PCR DNA HES Tag DNA n Cb PCR DNA i pH MgCl Taq DNA dNTPs 4 DNA PCR Eg PCR
48. RMP den RMP 3 RMP RMP 2 72 1 CODIS 13
49. DNA DNA 1 STR Short Tandem Repeats DNA DNA DD VNTR STR 2 VNTR STR STR 1 STR 1 STR RFLP 1 PCR 2 PCR TH STR
50. KE M EH 109418 nus z iode PA 19 1 2 2 47K 499ml 47K 400ml Tom 1 3 Pug M NE 1 2 10 1
51. 71 T G C fal 1 VWA 2 VWA D8 fal 3 VWA D8 D5 f 4 VWA D8 D5 TH01 5 DNA
52. DNA THO1 10 0 008 DNA 10 10 0 008 x 0 008 0 000064 700 000 1 2 DNA 0 0493 x 0 000064 0 0000032 3 A 10 2 DNA 10 10 E D3S 1358 THO 3 DEY 300 1 DNA DNA
53. 2 VWA D8 3 VWA D8 D5 4 4 VWA D8 D5 THOI ag DNA 3 A nul E C 6 2 61 2 DNA
54. gt 13 110 1 1
55. COIDS Combined System DNA CODIS PCR 13 1 8 32 STR 1 PCR 1 PCR PCR 53
56. Extension Taq dNTPs dATP dTTP dCTP X Genome T L 1 Genomic DNA DNA DNA Genotype Intron RNA dGTP 5 3 Locus loci Lysis la zr EC P Master Mix PCR dNTPs E Nucleotidess
57. 4 DNA PCR DNA PCR 5 POR PCR 3 DNA2 1 DNA DNA DNA ILesson 2 1 DNA DNA DNA
58. 5 D3S 1358 THOL D3S 1358 16 0 222 17 0 222 DNA 0 222 x X0 B AX 500 1 16 17 0 222 0 0493 D3S 1358 DNA 16 17 500 1 DNA DNA H Ej D3S1338 FDI DNA 1 A DNA 16 17
59. 13 gt 2 DNA 1 2 1 200 1 2 DNA STR DNA 3
60. A DNA En PCR DNA DNA NOT 13 DNA A A TH01 DNA 3 6 TCAT ccc LT IL I LL LL LI TCA 6 8 CCC TCA 3 E B THO1 DNA 5 10 CCC TCA 5 E CCCL__ ener al Fig 2 THO1 DNA A B THO DNA Fig 3 2 DNA THO1 PCR
61. CODIS Combined DNA Index System U 2A aun El DNA OF CODIS DNA 13 DNA CODIS US DNA CODIS DNA 69 DNA x3 14 Hare se X BARR LSPEC HUE CRS ICEL IL BREN K 50 DNA CORRICAMT SEAL DYZET IMIR DNA
62. 1980 DNA RFLP Restriction fragment length polymorphism analysis B RFLP 20 PCR DT DNA 2 DNA gt Ly X ASN O
63. ECR 4 DNA DNA Fast Blast DNA 69 DNA ILesson 3 1 PCR DNA DNA PCR DNA
64. 43 A DNA PCR lt DNA gt DNA Deoxyribo nucleic acid 4 4 DNA 4 3 1 Qi T 2028 O pe eR 3 NH TT v 1
65. OS STRs DNA 3 DNA ETT THOI STRs LOMB 1000 EJ SORE DNA ER OD 3 387 CODIS Combined DNA Index System CODIS FBI E
66. DNA RMP 3 fF ET RMP KH f VWA x f D8 x f DS x f THO1 A1 A2 p q Hardy Weinberg Hardy Weinberg p 2pq q 1 f A1A1 p f A1A2 2pq f A2A2 q A1 A2 A1 ATAI A1A2 p 2pq A2 A1A2 A2A2 2 2pq q ZEN AC SALATOS T BRE f A p A1A2 2pq dis STR
67. PCR H d nj BR DS 2 2 E A E 23 DNA DNA 2 PCR S 1 100bp 200bp Ef Te FO ET E IX DNA
68. Fig 5 2 13 STR 6 THO 6 3 7 THO 7 6 6 D3S1338 7 D3S 1358 DNA 16 17 DNA 7 4 THO DNA 63 DNA 2 4
69. DNA i 20u 401 PCR 2 0 05 U ul Taq 3 mM MgCl 1 6 mM dNTPs 1uM DNA PCR 1 0 023 U ul Taq 1 5 mM MgCb 0 8mM dNTPs BE 0 5uM PCR gt PCR DNA 2 1 FO PCR qe PCR 1 RH
70. CODIS 13 STRBase CODIS 13 2 xn 4 LI
71. ca LAL PCR CJ 1 DNA VBS 60 94C E DNA 3 G A DNA En Um f 1 2 DNA H ee
72. DNA THOU TU ARAA B 14 13 12 11 10 9 6 m e 6 4 G Fig 3 DNA Fig 4 2 TH01 Fig 4 THOI Fig 4 THOI 6 9 3 o N 427 Caucasians N 414 African Americans m 4 N 414 Hispanics 10 7 10
73. PCR TCAT STR DNA DNA 2 THO1 10 PCR DNA STRs TH01 DNA DNA PCR 28 A B THO Fig 10 2 DNA THO1
74. C T A G 2 DNA DNA DNA DUT FIE 5 3 DNA 3 5 gt gt Fig 14 DNA 45 A T Gi C 2 DNA fIise4 I RHO 1 3 1 3 517722 120088131875 I8 CE 77A 88 65 1 2 DNA DNA DNA DNA
75. DNA 1 2 DNA Short Tandem Repeat STRs STRs 11 THO DNA TCAT 4 CCC TCAT TCATITCATTCATTCA THO1 20
76. 2 ADAWCDH INH STRs STR DNA gt DNA mtDNA ERM AE 4 FH Em mtDNA DNA DNA mtDNA mtDNA 2 OO E mtDNA Clk7U PT ARIE OERE
77. DNA M 20 50bp DNA 5 dn 3 OH DNA A G CIT 3 HO 37KB AE OH 3 DNA DNA 2 46 DNA SAIL DNA
78. E TREK I LERZ T FE gt Ant opt z aN d DNA 20 4 100 4 El 1 1980 4 fragment length polymorphism analysis E KO iil Eft RRD E E
79. wa JHI Taq P 2 fi 4 m HH DNA THO1 JE dex DNA Ca ad ED Nerd E 35 E 1
80. 0 1 VAR ABICAM lt 10 E 1 DTT ET Ez SAILAR E2537 1 DNA 15 E 1 E PCR 1 FEL EREM DNA 1980 E Chain Reaction PCR DNA
81. 1 FGA 21 23 DNA DNA 7 THO D3S 1358 13 1 3 1 TH01 DNA D3S 1358 DNA 6 3 DRACO 16 17 FGA DNA 21 23 Fig 5
82. RMP C RMP 500 1 65 57 DNA Random Match Probabilities STR STRs TPOX 8 0 535 12 0 041 0 044 D3S158 16 0 222 17 0 222 0 099 FGA 21 0 185 23 0 134 0 050 CSFIPO 11 0 301 13 0 096 0 058 D8S1179 16 0 031 0 001 TH01 10 0 008 0 000 VWA 15 0 082 0 007 D18S51 14 0 137 19 0 038 0 010 D21S11 28 0 159 29 0 195 0 062 RPM 517x104 13
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85. BILL amp H gt 3 nj 2 1 ZUCIS EL CU SC 2 3 4m H E P C230 C8 A
86. Fig 19 STR 2 DNA 1 STR mu RFLP STR R LA PECHINO CENE ORO NOW EE R mtDNA d BL Fig 19 DNA 0 J gt DNA DNA Index PCR
87. 1 SNPs DNA 2 13 STR DNA ee61v5 AA ICH 1 2 SNPs SNP STR INDOOR STR SNP 52 f DNA
88. 2 PCR DNA dNTPs Tris 68 EAU 3 Taq DNA HEVET ATCG DNA DNA 2 DNA PCR DNA
89. zz zz zz zz 35 A B C D S i _ SS e Fig 8 Crime Scene Investigator PCR Basics PCR 2 DNA 7 3 BO 2 DNA 7 3 3 7 10 3 2 DNA 1 2 7 3 7 3 3 A 10 3 10 3 4 B 5 2 2 C 7 3 7 3 6 D 3 2 3 2
90. 2 BCT TREO a a 1 aE 3 1xTAE 2 30 L Ca fi nj cB 4 60 5 60
91. 25 PCR Lesson 2 PCR L 2 Lesson 1 PCR 5 Kp PCR A 7 i eT 3 G 10ul LD PCR 4 T aC 5 BAKBEN PV ESEAITFI
92. DNA Sc EAI L DHH KATALA VANAT oma TT BERKEL COE 8 DNA HARZET DNA DNA RNA Ha 1 20 4 uk welt T 1 1985 1 1 PCR PCR gt PCR 1 DNA 4 2 DNA f PCR Rb AUKLRS2 o0 g ff DNA DNA 2 DNA
93. 2 VWA 2 VWA D8 8 12 9 13 2 D8 2 D8 DS 7 11 10 12 2 DS 2 D5 THO1 7 11 10 12 2 THO1 2 THO1 1 1 1 4
94. 8 5 DNA 1 6 15 7 9 94 C 2 1 n 94 C 30 35 n 2 30 72 C pss 72 C 10 1E gt MMP DNA A DNA B DNA C DNA D DNA
95. 2 ASH DNA bp DNA 823 1 000bp bp kb 100 109 bp mb NN ONS NONON NN ON NOA2N NO NN Fig 15 DNA NANA DNA AA NM DNA DNA gt 98999299 9343 DNA i DNA DNA 5 3 1 R DNA 1 1 DNA 1 DNA 2 AGA DNA DNA
96. E E PCR 4 MELLT 1 E o PCR PCR POR gt PCR DNA DNA DNA DNA DNA PCR DNA
97. 3 ET 4 20ul 20ul 1 20ml 2 20m 3 A 20ul 4 B 20ul 5 C 20ul 6 D 20ul 39 5 6 100V 30 7
98. STR DNA JdMe DNA DNA PCR STR ion PRICLS FAP DE EERE SL ze E D NOE LR OER MONTORRE S meea uer DNA Fig 20 DNA PCR PCR DNA 7 Fig 21 PCR PCR PCR
99. Fig 6 THO1 ma em C D E LE 1 NN 57 a2 an 10 amp l e m pe cS a e Fig 6 TH01 DNA DNA E c 4 o TOX 3 ESAE IBS EEC mim 8 HEX4 A 32 ADA E
100. 30 gt DNA 2 DNA PCR 3 DNA PCR 4 5 6 DNA 31 Lesson 1 PCR PCR DNA Fe DNA DNA DNA eo a A PCR DNA gt 2 ASAD DNA 1
101. P 20 P 20 1 EE ey MMP 1 DR 4 Se tU UA 3 CS A B C D 1 PCR CS A B C D PCR DNA 6 0 2ml PCR 5 E DNA 20 1 A
102. E C DNA LCT DNA PCR DNA 5 Biotechnology Explorer FH Jorn as H A 4 25
103. annealing 2 DNA SHO 72 TaqaDNA DNA extension Taq 2 DNA DNA 2 2 DNA DNA 1 fig 16
104. AFLP 1 SNPs Single Nucleotide Polymorphisms 3 Fr 1 SNPs SSNPs Silent Single Nucleotide Polymorphisms 1 SNPs 1 2 biallelic STR SNPs
105. 3 4 1 9cm 6 ida 7 8 9 DNA 107 20 Wie 10 11 12 100V 30 1 DNA AKO 2mm 1 TAE pki E
106. Power of Discrimination 2 Primers DNA 3 30 PCR Reagents Restriction Fragment Length Polymorphism RFLP VNTR DNA DNA DNA STR DNA 2 4 STR STR PCR DNA
107. 10 PCR 4 RFLP 3 DNA 1 STR STR 1 2000 1 51 13
108. 1 1 RANZ DNA DNA 3 E PCR E DNA Taq Thermus aquaticus ADD 35 Y DNA 2 PCR bp lbp 1 PCR gt PCR
109. DNA gt 3 3 DNA 4 DNA d DNA 1 PCR FLET DNA 3 ZO DNA BLEU 4 ADK Pm AK J rvcv x3 3 H 7
110. DNA PCR DNA PCR DNA 1 4 30 3 lt gt Lesson 1 PCR DNA Lesson 2 PCR PCR MUS Lesson 3
111. TCAT Allele DNA Till A THOI DNA 3 6 TCAT CCC EE as TCA 6 CCC TCA 3 B THO1 DNA 5 10 CCCL__ I TCA CCC ee TCA 10 Fig 9 THO1 DNA STR gt DNA DNA
112. HH d E 0 05 ug Fast Blast DNA 1 Fast Blast DNA Lesson 3 DNA lt gt 1 500 1 000 700 500 400 300 200 100bp 8 15 Yu DNA ORB 10 3 1 Fast Blast DNA amp Yu
113. PCR PCR P 20 2 20ul H BPH AHMAR HM Lesson 2 PCR 200 1000 DNA DNA 3 CIS CI G 1 1 1 5 ml 16 P 200 1 P 200 1 P 20 8 P 20 8 Fast Blast DNA 1 Fast Blast 1 3 5L 1 8
114. DNA DNA DNA 5 10 DNA 10 DNA 65 E Allele Annealing DNA o an CODIS Combined DNA Index System DNA
115. E J L 408 DNA 1983 DNA DNA DNA TN 1985
116. y LOVEE DNA 8 ch ADT 3 d oo DE SCORES C671 E 3 10 1 DNA Bike Beni DNA Bitte BERT EGER 3 13 Fl DNA
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118. DNA DNA PCR DNA 3 PCR DNA THO1 E PCR PCR PCR
119. H T 15 gt 3 1 Lesson 1 PCR at 5 DNA 1 A DNA 1 B DNA 1 C DNA 1 D DNA 1 300 350 ml G 1 G 1 P 20 1 P 20 1 1 E Fast Blast DNA 1 1 lt TAE gt TAE 1 2 30 1
120. bah uu PET nif FOIL SAR IRKE CO uli SF TORRE MICHA MILO CH 1 AE FR gt Ic DNA 7 E DNA
121. RMP 63 1 CODIS 13 R CODIS 13 STRBase x 2 64 D PCR
122. 7 5 4 3 2 1 2 3 E ED w X G 10 G DNA eine H 1ml Fig 8 Fast Blast DNA DNA gt DNA F GR
123. DNA Cofactors 1 OO Mg Denature 2 DNA TDS PCR dNTPs DNA 4 dQATP dTTP dGTP dCTP Ethidium bromide Eukaryotes DNA DNA R E TaqDNA Exon RNA
124. E G ae DNA 1 PCR inset 0 G cd H DE OPEV ALU CAI gt DNA Fast Blast DNA Zt H 1 G Ed Fast Blast Wi o dA yu ET Nd toe
125. 1 2 20uL 1 8 X 20uL 166 0513EDU 2 20uL 8 8 6L 1 8 1 8 1 Basic 164 5050JA 2 4 xt THA AP VERGE L727 GT 170 4406JA 1 8 B 1 200 300ml 500ml IL 500ml 1L 8 PCR 8 50 7 vit RR 170 2984 8 Standard Content Standard A Students will develop abilities to do scientific inquiries Students will develop understanding about scientific inquiry Content Standard C Students will develop an understanding of the molecular basis of heredity Content Standard E Students will develop an understanding about science and technology studies Content Standard F Students will develop an understanding of sc
126. P 1000 P 200 45 gt 3 3 Ep EE a gt t vol V oo oo oo T Omm ET 1 10 1 DNA 2 BES EY DNA
127. DNA EE YC 4 DNA HL PCR 1 DNA PCR ED PCR HAE PCR DNA fff i OARS DNA DNA gt 30 99 5 DNA lt 0 5 2 Lii 1H
128. TH01 1 THOI 8 ACT 1 8 lt 1 1 8 c1 X hi 6 5 5 1 8 CH 2 2 fll
129. 4 4 STR 2 1 STR 60 K 1 STR DE VWA D8 D5 THO1 i 1 VWA
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131. 1 1 0000 2 20nul MMP 3 A 15 1 3 MMP 1 000ul 20ul 12001 MMP 8 2 DNA 25pl CS 8 3 A DNA 25pl A 8 4 B DNA 25pl B 8 5 C DNA 25u C 8 6 D DNA 25pl ID
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136. TaqDNA polymerase TaqDNA Thermus aquaticus DNA DNA PCR Template DNA Variable Number of Tandem Repeats VNTRs DNA DNA VNTR 67 F DNA
137. A DNA 20 u1 B DNA 20 1 C DNA 20 ul D D DNA 201 g D in PCR 2 35 MMP 20u MMP 20u MMP 20ul MMP 20u MMP 20u ELE 3 20ul P 20 DNA DNA 20 1 CS PCR DNA 4 20ul P 20 MMP 20m
138. DNA 3 RA k RNA 66 E mRNA DNA RNA CH Oligonucleotide DNA RNA PCR DNA Polymorphism
139. 5 30 60 C 3 d ELET 175 3 60 C FIR E
140. 6 mtDNA di da mtDNA mtDNA 1 Amplified fragment length polymorphism AFLP RFLP PCR H STR mtDNA 1 HECHT DNA PCR AFLP RFLP AFLP
141. PCR THO1 F DNA DNA 33 Lesson 1 gt PCR 1 PCR DNA 2 PCR 3 4 E DNA PCR 5 PCR 34 Lesson 1 gt DNA 6 MMP AD DNA PCR PCR
142. DNA DNA DNA DNA o gt Dy 24 PCR 1 PCR 5 CS A B C D PCR 2 DNA PCR T Uu DNA 20 ul PCR DNA CS
143. PCR DNA DNA PCR DNA 1 DNA DNA PCR PCR 2 PCR
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145. DNA ae THO Fig 12 4 HEL DNA Xt aeons Rid A Nel Qc x E38 1 1500 1000 700 500 400 300 200 DNA PCR CS 5 ie 2 5 2 r DNA MECH DNA
146. DNA 2 PCR Taq DNA PCR DNA DNA Taq DNA 2 3 DNA PCR 1 5 EDITING Crime Scene PCR Option Add Del Done Fig 7 PCR PCR ION a uo c PCR
147. 1 E 2 IxFast Blast DNA LETA 3 1 100 EIRO Fast Blast DNA 5 2 Fast Blast DNA 10 3 100ml 3 2 4 E Lesson 3 DNA Gu
148. 13 RMP 2 10 2 gt DNA DNA
149. TCAT 4 CCC CAT TCAT TCATITCATITCA THO DNA S nm A THO1 DNA 3 20 TCAT Allele 7 oyn 62 CCC TCA B THO1 DNA 5 10 CCC I I ITCA Fig 1 THO1 DNA DNA PCR HB DNA PCR R TH01 1
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155. 1985 DNA Variable Number of Tandem Repeats VNTR DNA VNTR RFLP RFLP DNA DNA
156. 70 1 8x1 8 1 64 7 13 1 THO THO 13 1 THO1 64 1 13
157. FVM Shi R Crime Scene Investigator PCR Basics PCR DNA Taq PCR DNA DNA DNA 4 DNA DNA 1 DNA 13 DNA DNA
158. 25ul 4C 2 SE 5 G 4 G 2 TKN 7 7 TAE 3 4 30 1 Fast Blast DNA Fast Blast DNA 15
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160. yd E CODIS 13 E THREE UE 2 STRBase http www cstl nist gov biotech strbase Data from NIST US Population SamplesT Allele Frequencies published in the Journal of Forensic Science 1 2 3 ILT KH 3 2 518
161. 1 E 1 HATE 1 1 3 Lesson 1 PCR PCR 40 PCR 40 2 0 ml 49 DNA A DNA B DNA C DNA D DNA P 1000 P 200 P 20
162. DNA DNA 3 Fig 17 PA a yg SASS Tt 1 V 94 C DNA OEE PP 2 l 523C A EEE y 72 C DNA SAO 4 l 94 C DNA 2 DNA l 52 C 35 Fig 17 3 DNA DNA 8163 XX n DNA
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175. G P 20 P 20 2 1 Or Fast Blast DNA gt 1 1 3 1 2 1 1 1 1 300 350 ml 60 ul 25ul 1 1 1 2 Lesson 1 PCR 5 3 10u G LD PCR
176. 0 DNA PCR DNA DNA HUO DNA 1 A P TCAT H 6 3 A DNA Prid DNA 10 000 Al Br xw STRs DNA Crime Scene Investigator PCR Basics THO
177. A DNA E DNA PCR DNA 1 1 B RFLP PCR BRL 2 JE fB E E FIRA RFLP Restriction H H E M EX E 2 DNA H ia
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179. DNA DNA DNA DNA pu 1 DNA DNA 2 1 PCR 2 AGA DNA 1 DNA 3 DNA sad DNA Fig 11 DNA 3 Fig 11 DNA PCR
180. H 1 DNA DNA DNA
181. HT 60ml 100ml 100ml 8 Fast Blast m OS te RD e E pues 7M uae SyberGreen UV 0 05ug ml
182. RFLP 1988 FBI RFLP 1990 STR PCR 1992 FBI DQAI STR 1994 DNA DNA 1995 0 J DNA 1996 FBI mtDNA STR 1997 13 STR 1998 FBI CODIS STR DNA 2001 DNA 8 2004 69 DNA 62 14 2004 DNA X CODIS DNA gt DNA
183. ertet tette neret re tete ac ettet tute ite s 21 Lesson POR esos e te ette et ep bv o ate ELA Sek dat c tdt OK cius 21 Lesson PCR PE EE PKB asc e eee n eb e rante od e mre 4s 23 Lesson3 DNA ASSO SST aate tenete nte ener ergeben edant eda aerae E 23 BAIANA REPRE OREL DURS DR DEEP 25 RH HE Guts de TS CE Nen aL e meis DIS A seek Lun NU E a Ue UE e Mae MT nes Gah RE i e ces 27 OE RN Ni NE EE M 27 BT OT A E 31 besson l BORA ES Hl sisi on ors Fe bcc ea e rette Satta de eb eet ctc avid es tee b RE i 32 UB hessonl s POR Bs AB SG SI 34 Lesson E ES E 35 Lessom2 PCR kk AG 37 lt T V 38 SS 39 Lesson 3 DNA kk 41 lt A Desson32 sean re reet ei tere lt A 42 BISRA DNAVPORIS UNE esee teat pep n eedem alas dal t ete 1 44 B kkk 51 C STR RMP kk 59 IRE ASTRO i RISO TOU lo BU edet RR a eI EE 59 I RCRUM ANIM ed UTES NENNEN CI XR 62 BIRD PCERE RETE TE ueteri eh EE E e ER en ta t n i tete tendi 65 E RR 66 DE de S EES 68 G C kk 72 EC SINE C OC MNT NET TT E 74 gt
184. ience and technology in local national and global challenges Content Standard G Students will develop an understanding of science as a human endeavor Students will develop an understanding of the nature of scientific knowledge National Science Standards Fit to Standard Students will perform an experiment using sophisticated procedures Students will apply the results of their experiment to scientific arguments Students will understand that genetic information is passed from parents to children Students will perform an experiment using sophisticated procedures and will gain an understanding of technology as it is used in forensic Students will learn about how technology is used in identification of victims in disasters how it is used in the courts and how technology can be used to reveal familial relationships Students will work together in teams to achieve a common goal Students will perform their own experiments and come to their own conclusions based on their experimental observations DNA gt DNA DNA DNA 4 v DNA
185. on 2002 9 OJ Simpson trial evidence http www law umkc edu faculty projects ftrials Simpson DNA htm 10 Short tandem repeat DNA Internet database Biology and Technology Behind STR Markers http www cstl nist gov biotech strbase 11 Strengths Limitations and Controversies of DNA Testing http arbl cvmbs colostate edu hbooks genetics medgen DNAtesting DNAtest_pcs html 12 Thieman WJ and Palladino MA Chapter 8 DNA fingerprinting and forensic analysis pp 169 184 in Introduction to Biotechnology Pearson Benjamin Cummings San Francisco 2004 13 US Dept of Justice Federal Bureau of Investigation The FBI s Combined DNA Index System Program CODIS http www fbi gow hq lab codis 14 Voter Information Guide California Proposition 69 DNA Samples http voterguide ss ca gov propositions prop69 title htm Careers in Forensic Sciences Lorenz K Think You Want to Be a CSI http msn careerbuilder com Custom MSN CareerA dvice 456 htm siteid cbmsnhm4441 amp sc extcmp JS cjl jan05 hotmaill amp GT1 5938 US Department of Justice Office of Justice Programs National Institute of Justice Education and Training in Forensic Science A Guide for Forensic Science Laboratories Educational Institutions and Students June 2004 http www ojp usdoj gov nij pubs sum 203099 htm 74 116 0014
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