1step RT-PCR ノロウイルスG1検出試薬キット 取扱説明書
Contents
1. 10 M 8 X174DNA Hinc I y I C 1 1 2 2 i LC A tM 4 10 uL 100 V 25 TAE 1 2 1 2 W PCR SYBR Green 1 TEL 075 823 1351 http www an Shimadzu co jD deneral contact contact htm 2015 Shimadzu Corporation All rights reserved2. RT PCR 2 MA RT PCR 1 RT PCR 1 NV Reagent A 25 UL NV Reagent A 25 LL RT Enzyme 0 25 LL RT Enzyme 0 25 LL NV Reagent B 4 5 LL NV Reagent B i 4 5 LL PCR Enzyme 0 25 LL PCR Enzyme 0 25 LL Total 30 LL A 125xSYBR Green I 1 uL ea CE Total 31 LL NN a 2 1 20 uL PCR 1 RT PCR 31 uL 51 uL 3 PCR 1step RT PCR 2 1 20 pL PCR 1 RT PCR 30 uL 50 uL 3 PCR 1step RT PCR 1step RT PCR 1step RT PCR 40 4
3. DNA 1step RT PCR G1 1 RT PCR Vs Reverse Transcription RT PCR 3 DNA I C TM Ampdirect SYBRB Thermo Fisher Scientific No Sample Treatment Reagent 1
4. 8 i 6 7 1 8 9 1 5 10 W V 9 0 1 1 mL 1 10 PCR 2 10 000 rpm 5 11 PCR 3 PCR Sample Treatment Reagent 19 uL 1 pL 12 dl 1 5 mL 2 mL 20 UL I MN A ME A 4 90 5 5 II 14 2 15 125 x SYBR Green I 2 16 1 x TE a SO 1
5. RT PCR 251 bp EE oO 00PD x 1 RT PCR 10 4 RT PCR GVP 9600 lt ET a 2 lt 90 0 EE EE 78 5 84 5 x 7 2 3 RT PCR
6. 17 RE II SYBR Green I PCR 1 2 125 x SYBR Green I Thermo Fisher Scientific SYBR Green I nucleic acid gel stain 10 000x TE 80 125x SYBR Green I Thermo Fisher Scientific O I 1 RT PCR 1 RT PCR 2 I 1 RT PCR 1
7. 912 HL 4437525 NV Reagent A 1 1 2 mL RT Enzyme 1 12uL NV Reagent B 1 216 LL i PCR Enzyme 1 1 12 LL 48 tests 20 53M NO a I L RNS RT ER H 20 1 PCR 2 PCR 3 4 PCR 1 6 ON a 5 10 000 rpm
8. 241 09514 1 sg SHIIM U SDS SDS lstep G1 Ampdirect Technology G2 1step RT PCR i G1 amp G2 P N 241 09500 91 1step RT PCR lstep RT PCR G1 G2 P N 241 09500 93 1step RT PCR Norovirus G1 Detection Kit P N 241 09500 92 2 a PCR yikag li
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