蛋白質科学会アーカイブ ディスポーザブルカルチャーバッグを利用した
Contents
1. 3L 2 5L 1 10 30 3 1 8L pH DO2. Minimate Pellicon XL Vivaflow Pl
3. 5L 2 5L 10L 5L 5 2 BMMY
4. Pichia pastoris 1 AOX1 Saccharomyces cerevisiae S cereVS 2 x cerevisiae 1
5. glycoengineered strains lgG 2 7 e078 2014
6. CB 5 2 5L CB 10 5L 6mm 9mm HR340MB 0 20m LABODISK 50JP 2 L BMGY 29 9
7. 3 6 L 0 20um 0 22 um GE 8 L
8. AOX1 3
9. 24
10. 2 BMMY BMMY 3 6 24 7 7 e078 2014 O18 5 3 pPICZvwA
11. 7 e078 2014 1 1 e006 2008 2 Damasceno LM et al Appl Microbiol Biotechnol 93 31 39 2012 3 Invitrogen EasySelect Pichia Expression Kit Catalog no K1740 01 4 Bjioneer series 5 1 e018 2008 6 Muraki M BMC Biotechnol 14 19 2014 7 CULTURE BAG 8 89 570 83 2011 10
12. 3 pH BMGY BMMY pH 100mM pH 6 0 BMGY O0 5 pH BMMY pH 7 e078 2014 3mm
13. SDS PAGE 3 SDS PAGE 3L 500mL BMMY 7 e078 2014 Oh 24h 48h 72h 96h M FCFC FC FC FC 3 3 SDS PAGE 6 Fig 5a F 500mL BMMY 3L C 2 5L BMMY 5L 2 7
14. _BR 32FL 1L 1 L min DAP 12S 7 e078 2014 RK1710 1 0 BMGY 3L C 65
15. 100g g mL 2 YPD pPICZa A S cerevisiae a amp Kex2 Ste13 Fas 2 5L 6 1 3mm 3 3L 500mL BMGY 29 5C 300rpm 20 2 50mL 8 25mL
16. 7 e078 2014 Pichia pastoris Secretory production of recombinant proteins in methylotrophic yeast Pichia pastoris using a disposable culture bag Biomedical Research Institute National Institute of Advanced Industrial Science and Technology Michiro Muraki 2014 6 13 2014 7 4 2014 7 6 2 5L 5L
17. 10L 6 5L 4 1 2L 150 DO pH 1 2L 200 4 2 5L 5L 30C
18. YPD BMMY D
19. 1 1 1 34 100mM pH6 0 4x10 9 D BMMY BMGY 0 5 KD PW100 O18 5 3 7 e078 2014 BMGY BMMY 1 BMGY 2 BMGY
20. 2 3 1 5L 1 2 A B 2 3 6 24 0 596
21. 2 4C 5500rpm 20 0 20m 7 e078 2014
22. gt gt 5800G 2 M 3 10mL Sh 5800G 2 5L 1 7 A B 6 Fig 1b 7
23. e078 2014 BMMY 10mL BMMY 2 5L BMMY 2 BMMY
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