THUNDERBIRD Probe qPCR Mix
Contents
1. 13 PCR 90 slope lt 3 6 PCR 4 2 PCR PCR PCR PCR Ct Ct PCR2. HPLC TAMRA non fluorescent quencher EDTA
3. HPLC 4 DNA DNA cDNA genomic DNA plasmid DNA virus DNA DNA a cDNA cDNA PCR 10 PCR PCR 109 PCR ReverTra Ace qPCR RT Kit Code No FSQ 101 PCR
4. 0 2 0 6 pM 2 50X ROX reference dye Applied Biosystems 1 ROX reference dye Applied Biosystems 7000 7300 7700 7900HT StepOne 1X 1 50 StepOnePlus Applied Biosystems 7500 7500Fast Agilent Technologies A Roche Bio Rad BioFlux Line Gene Realtime PCR Master Mix Code No QPK 101 ROX 1X 5 2 PCR p 3 2
5. 2 QPS 101 QPS 101L QPS 101T THUNDERBIRD roogoe Sm 5ml 1ml Probe qPCR Mix 3 1 67mlx3 5mlx1 1ml x 1 20 C 50X 2 8 C 250Ll 250Ll 50Ll ROX reference dye QPS 101X5 QPS 101 5 THUNDERBIRD Probe qPCR Mix dNTPs Mg rTaq DNA DNA 2X DNA cDNA genomic DNA virus DNA plasmid DNA 1X ROX passive reference dye 20 C 3 2 8 C
6. RNA 5x RT Buffer RNA 2 Nuclease free Water XHI 5x RT Buffer 2 HI RT Enzyme Mix 0 5 Hl Primer Mix 0 5 Hl RNA 0 5pg 1pg Total volume 10 Hl 3 37 C 15 98 C 5 0 4 C 20 C PCR RNA
7. plasmid DNA 1ug plasmid DNA 9 1 x 10 plasmid DNA kb 4 1 TaqMan Probe 50 ul 20 ul 50ul 20 X Hl X HI THUNDERBIRD Probe qPCR Mix 10 ml 1x Forward Primer 6 pmol 0 3 uM Reverse Primer 6 pmol 0 3 uM TaqMan Probe 4 pmol 0 2 uM 50X ROX reference dye 0 470 04ul 1x 0 1x DNA Y HI 20 HI 9
8. ROX 4 ROX 1X qPCR Mix 50X ROX 5ml 200pl QPS 101L 1 67ml 66 8ul QPS 101 1ml 40ul QPS 101T ROX 0 1X qPCR Mix 50X ROX 5ml 20u QPS 101L 1 67ml 6 7ul QPS 101 1ml 4ul QPS 101T 3 1 PCR PCR 2 20 lt 30mer GC 40 60
9. 20 C 10 50X ROX reference dye Applied Biosystems 4 20 C 2 8 C 20 C ROX reference dye 50X ROX reference dye THUNDERBIRDY Probe qPCR Mix THUNDERBIRD Probe qPCR Mix ROX
10. 20 b 9enomic DNA virus DNA genomic DNA virus DNA 50ul 200ng c plasmid DNA plasmid DNA plasmid DNA DNA DNA DNA PCR yeast RNA
11. NTC multiplex PCR multiplex PCR 50X ROX reference dye 50XROXreference dye 4 1 50X ROX reference dye
12. PCR 45 60 15 7 SYBR Green PCR 1ml x 1 QPS 201T 40 SYBR Green PCR 1 67ml x 3 QPS 201 THUNDERBIRD SYBR qPCR Mix 200 5ml x 1 QPS 201L 200 THUNDERBIRD SYBR qPCR Mix 50X ROX reference dye 50ul 1000 QPS 201X5 QPS 201 5 cDNA PCR cDNA ReverTra Ace qPCR RT Kit 200 FSQ 101 PCR cDNA ReverTra Ace qPCR R
13. 12 6 DNA PCR PCR cDNA PCR DNA DNA
14. PCR 2X ROX Realtime PCR Master Mix Code No QPK 101 gt PCR PCR 1 PCR 2 PCR 3
15. DNA DNA
16. 4 50X ROX reference dye Applied Biosystems Agilent Technologies ROX 5 Taq gt TaqMan probe SYBR Green
17. Ct PCR 110 slope gt 3 1 PCR Ct Ct PCR Ct PCR DNA DNA PCR DNA PCR
18. 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
19. 1 Analysis Mode None Cycle Program Data Analysis ucds ee F Quantification Bamal il Temperature Tarqets Target Temperature CI Incubation Time hrs min sec Temperature Transition Rate s Secondary Target Temperature C Step Size C Step Delay cycles Acquisition Mindg ROOT 2 PCR Analysis Mode Quantification 60 C Acquisition Mode SINGLE Cycle Program Data Analysis Mode None a Quantitication Gyeleal 40 Temperature Targets Target Temperature CI Incubation Time hrs min sec Temperature Transition Rate s Secondary Target Temperature C Step Size CC Step Delay cycles Acduisitinn Mode WE Ri 0 GE 10 3 Analysis Mode None Cycle P
20. 5 15 3 56 64 C 4 300bp 30 30 45 60 30 Applied Biosystems 7000 7300 31 7500 35 2 1 Applied Biosystems 7900HT 2 2 2 TaqMan
21. DNA DNA DNA Yeast RNA 4 2 PCR PCR 14 no template control
22. 200bp 80 150bp DNA meltting temperature Tm 60 65 C HPLC OPC 3
23. Probe Applied Biosystems 7900HT 1 Instrument Thermal Profile Sample Volume uL 45 Setup InStruimert Thermal Cycler Pratacal 2 Mode Fast Standard C9800 Emulation Sample Volume L 25 Thermal Profile Auto Increment Ramp Rate Data Collectian Stage 1 Stage 2 Repeats lo add Cycle mdd Hold add Step Delete Steh dd Dissociation Stage 2 Data Collection 3 2 2 Roche LightCycler 1 1 3 5 TaqMan Probe Roche LightCycler 1 1
24. 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos 5 538 848 5 723 591 5 876 930 6 030 787 6 258 569 5 804 375 claims 1 12 only and 6 214 979 and corresponding claims outside the United States No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchasing licenses may be obtained from the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA LightCycler Idaho Technology Inc Roche Molecular Systems Inc TaqMan Roche Molecular Systems Inc SYBR Molecular Probes Inc 1 THUNDERBIRD Probe qPCR Mix TaqMan probe
25. T Master Mix 200 200 Fso201 201 PCR cDNA DNA ReverTra Ace qPCR RT Master Mix 200 with gDNA Remover FSQ 301 1 PCR CodeNo 0 5ml x 2 40 QRT 101T 1 PCR 0 5mlx 5 RNA direct Realtime PCR Master Mix QRT 101 100 SYBR Green 1 PCR MA g77ec7 SYBR Green Realtime PCR Master Mix A MA4 g7ecf 50mM Mn OAc 50ul 500 QRT 101X5 QRT 201X5 QRT 101 QRT 201 5 http www toyobo co jp bio 16 QRT 201T QRT 201
26. TOYOBO ldeas amp Chemistry THUNDERBIRD Probe qPCR Mix Code No QPS 101 QPS 101T QPS 101L TOYOBO CO LTD Life Science Department OSAKA JAPAN A4195K 1 rrr 2 ee 3 5 RNA cDNA 4 Oe 6 7 NOTICE TO PURCHASER LIMITED LICENSE A license to perform the patented 5 Nuclease Process for research is obtained by the purchase of i both Authorized 5 Nuclease Core Kit and Licensed Probe ii a Licensed 5 Nuclease Kit or iii license rights from Applied BiosystemSs This product is an Authorized 5 Nuclease Core Kit Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 079 352 5 789 224 5 618 7 11 6 127 155 5 677 152 5 773 258 5 407 800 5 322 770 5 310 652 5 210 015 5 487 972 and claims outside the US corresponding to US Patent No 4
27. TaqMan Probe PCR 95 C 20 lt 60 PCR 95 C 1 15 40 cycles 60 C 30 60 Data Collection 1 DNA 60 2 Applied Biosyste
28. ms 20 Applied Biosystems 7500Fast TM 30 Roche LightCycler 1 x 2 0 Applied Biosystems 7700 7500 7900HT 60 StepOne StepOnePlus W Agilent Technologies BioFlux Line Gene 2 PCR PCR 15 3 PCR PCR Applied Biosystems 3 Applied Biosystems 7500Fast Roche LightCycler 1 x 2 0 Applied Biosystems 7700 7500 7900HT StepOne M StepOnePlus W Agilent Technologies BioFlux Line Gene
29. rogram Data Analvsis Mbde RN Quantification ER Temperature Targets Target Temperature CI Incubation Time hrs min sec Temperature Transition Rate CA s Target Temperature C Step Size CC Step Delay cycles Acdulsitinn Mode RE 4 11 5 RNA cDNA cDNA PCR ReverTra Ace qPCR RT Kit Code FSQ 101 PCR cDNA ReverTra Ace qPCR RT Kit PCR cDNA 1 RNA RNA 65 C 5
Download Pdf Manuals
Related Search