応用生物実験の基本操作（Ver. 1.52）
Contents
1. 2 4 32. 1H 2 9 2
3. 2 8 4
4. 3 6
5. 2
6. 20 2 9 3 1 2
7. 4
8. 8 2 5 2 4 2 1 1
9. 2 8 6 2 8 4 2 8 5
10. 1 g 10 g 2 g 1 g 2 2 8 3 1 3 1 2 3
11. 6 2 lt 3
12. Rt 2 2 7 1 1 2 3 4
13. 3 7 2 2 3 107mL
14. 27 3 2 3 1
15. 1 2 8 3 6 7
16. 2 H 13 3 4
17. 6 6 7
18. 3 10 2 1 1 3 10 2 02 1 Tl 2 50 60 3 10 2
19. 23 9 2 4 3 6 O 0 1 1 M
20. 3 3 3 1
21. B 2 6 3 1 B 1 2 3 4 5
22. 2 2 4 2 2 5
23. 160
24. 2 15 3
25. 3 4B a b 1 cm 1xg 10m 1 10 000xg b 1 cm 10 1 2 1 2 1 2 1 0 A
26. 2 1 2 1 7 1 2
27. 2 13 3 12 20 A 12 20 kW 4
28. http wwwr hitachi koki co jp himac supportm tube htm http www nalgenunc co jp htmlinfo shtml http www assist sar co jp 1 8 9
29. 7 1 1 4 160 3 8
30. 17 10 1 1 g 2 3 5 mL 3 15 mL 10 mL 4 1 100 500 pL 20C
31. E co7 3 4 11 3 9 2 3
32. 4 Pk 0 5 0 1 3 2 1 1 9 sin 6 2
33. 3 DNA 50 ml 12C E bd 4 Mupid n H buffer L buffer Ig DNA c 1 9 1 M NaCl 13 29 3 10 2 pH 0 1 M HCI NaOH pH
34. H 1
35. 2 1 3 2 1 4 1 2 3 1500W 15 A 6 A
36. 2 cm 2 2 3 500 mL 1 2
37. 1 3 4 2 8 1 0 1 2 1 2 9 i
38. H 2 2 1 2 1 1 2 4 2 1 2
39. 1 NYY 2 3 4 5 mL 2 2 6
40. 2 5 2 1 3
41. 6 E 2 8 6 2 8 2 1 pH 3 E 2
42. 1 mL 350 4 000 4 000 15 13 4 100 1 800 Corning 50 Cat No 430304 1 800 6 000 Cat No 430290 430291 430828 430829 430522 15 1 800 Cat No 430053 430055 430788 430789 6 000 Cat No 430052 430766 430790 430791 430630
43. pH 7 pH 4 pH 9 pH 10 pH pH pH pH 2 6 86 15 C t H Wr x 3 6 92 pH
44. 6 7 11 1
45. Beckman RI H 1 3 2 7 3 UV
46. 3 6 3 6 1 1 13 173 N 9 AU RY 3 6 2 1 2 3
47. 6 7
48. 1 1 2 8 4 4 3 2 3 10 2 20 30 3
49. 0 5 7 8 9
50. 3 8 3 3 8 3 pH 0 pH 8 85 23 8 08 pH 2 12 0 2 pH pH 1967 pH 8 0 1 M Tris HC1 100 10 mM 0 pH
51. pH 8 pH KC1 pH 2 pH RS 10 pH 3 8 2 1
52. Vol 55 No 7 2000 Vol 36 No 10 2001 2009 ww a 9 30
53. EA 1 1 2 7 1 f
54. 15 A 6 A 6
55. E No No 1 2 2 4 4 4 A
56. 3 Ts E E
57. 3 30 1 1 000 2 000 rpm 5 10 ON 6 6 1 2 8 3 4 5
58. 19 2 3 EMS 0 2 mL 3 EMS hh 6 i 9 8 mL 1 2 8 6 1
59. 18 2 1 10 1 2 3 4
60. 3 2 2 1 2 3 3 3 3 3 1
61. 2 20 21 pH pH 7 NarHbPO Na HPO NaHPO4 NaH2PO NasHPO4 NaHPO4 pH pH 4
62. 2 2 2 1 2 3 2 cm 3 cm 5 5
63. D 8 9 3 5 3 5 1 2 2 1 3 5 2 24
64. 3 3 4
65. ET 3 7 2 1 3 107 E 3 109cm 25 i No 3 4 10 10
66. 1 1 3 10 2 3 2 2 1 2 3 10 2 3
67. 3 1 1 2 4 1 9 60 90 2 C pH 90 DO
68. 10 10 A N N Noe EAR A 7 2 13 173
69. 3 2 6 2 1 3 15 2 B
70. 3 4 4 i o PF 20 25 pL 3 4 3 1 E 2 3 1 E 1 2 3 3 4 4
71. 2 3 5 2 7 DNA DNA 1
72. 5 20 L 10 m 2 2 2 2 1 1
73. F Molecular Clonins 2 E8 1 Amberlite XAD 16 O Molecular Cloning 2 E9 Amberlite XAD 16 1
74. 3 18 1 2 8 1
75. 1 pH 4 http www bio eng osaka u ac jp Sfbj wakate 4 2 http biochem2 umin jp contents manualindex j html http Www k erc pref kanagawa jD CAS No http WWW yk rim orjp aisoaimolbio html http Www biwa ne jp fumika law htm 6 2 K
76. E gt V 1 2 H 3 4 3 1 HH 28 5 3 10 3 3 10 3
77. 2 3 1L 1 4 5
78. 3 1 2 1 1 20
79. 2 8 7 May cause cancer Mutagen Flammable Combustible Harmful Harmful by inhalation Corrosive Lachrymator Irritant Cause irritation Do not breathe fumes Avoid breathing dust Store desiccated Store below C Store at room temperature Store under nitrogen Air sensitiVe Protect from lght C 2 9 2 9 1
80. pH 5 3 3 pH pH 1967 HHH 26 7
81. 2 2 3 2 1 2 3 80 C 100C 2 D 2 2 A B C
82. 0 x QO O QO x x QO QO A orX orX 3 10 ZzE pH Q BE O y 3 10 1 A 1 Bs V Q X A W V x
83. 3 2 1 8 1 2 2 1 9
84. p p 1 20 1 21 0 01 1 00 1 10 0 11 3 4 rr 2
85. Tris Glycine 6 7 6 9 2 1 2 6 CBB 1 62 5 mM Tris HCl pH 2 pH 6 8 pH 10 k
86. 1 EDTA ATP 4 EDTA free acid 1 4 5 3 2 NazHPO 7 12 3 2
87. 2 1 2 2 40C 3 SDS TEMED 1XTAE 12XTABE
88. 2 4 5 1 100 000 rpm 3 DNA F 1 2 L F 4
89. 5 1 0 10 11 Ek DD 1 2 2 2 2 2 2 3 3
90. Q 4 Q 5 Q6 Q 7 Q8 Ww 2 4 1 8
91. 12A 10 12 1 SDS TEMED A B a 0 i C 2 0 30C
92. HH 8 B
93. 2 5 1 E 2 1 5 1 2 1 2 3 gt 4 gt 3 2 1 6 1
94. 3 3 FE 3 7 3 1
95. 2 3 5 2 2 8
96. 2 2 4 4 2 2 7 5 5 2 2 1 2 3 2 2 6 2 3 1 1 4 2 3 1 4 5 70 2 3 3 2 6 70C 0
97. 2 3 4 1 pH pHz6 2 A 2 B 2 9
98. 2 3 8 pH 3 8 1 1 3 4 pH
99. 4 2 1 Merk Index 2 3 4 3 4 1 1 2 3 4 3 4 2 1 2 1 Nm
100. 2 o 1 MD 1mA 1000 V 2 3 10 2 1
101. D 60 70C 6 3 1 2 300 mL 230 mL
102. 2 2 2 1 0 1 3 3 2 1 20C 1 1 2 3
103. 2 3 tk 3 4 5 6 Ml 2
104. 2 3 1 2 3 2 6 2 6 1 1 3
105. 1 8 2 000 rpm 2 4 7 know how 1 R p o p n ram r 9 n 6 Ra 1 2 1 2 1 44 12 2
106. 1 2 A B ES 121 10 20 7 2 3 3 1 2 C
107. 8 2 8 5
108. pH 10 w pH 20 80 3 3 1 3 1 2 3 M 50 w y 2 TE 20 3 2 5 6
109. 3 1 2 50 1 1 1 1 1 2 4 A B 90 Q Tmax 4 1 2 90 6 3
110. 4 7 14 1 2 3 4 5 6 1
111. 1 5 EE 2 2 1 5 2 4 6 clinical centrifuge 4 1 2 4
112. 2 3 1 1 3 4 4 Ed HL 3
113. 10 2 4 4 1 2 F
114. 1 6 2 5 3 1
115. 3 1 4 90 1 3 1 2 70 C 3 70 or 4 2 4 Q 1 Q 2 1 Q 3 1
116. dithiothreitol O O 3 4 4 7 4 B
117. 3 7 3 7 1 1 2 1 2 3 4
118. 2 DNA 2 8 15 3 245
119. 3 1 1 1 1 cm 0 1 1 pg Molecular Cloning E1 E2 0 1 BSA 3 30C 7 4 mgL 0 23 mM 1 4 1 1 mM 2
120. 6 6 7 8 9 1 2 3 2 5 1 1
121. pH H 10 1L 1 10L 900 mL 2 90 mL 800 900 mL 3 4 W1 3 3 pH 1L 6 W2 7 W1 W2 9 1 810 mL 900 mL 3 9 3 9 1 1
122. 1 2 1 2 3 3
123. 2 1 22 2 3 2 4 2 6 2 7 2 8 2 9 3 3 1 3 2 3 3 3 4 3 5 3 6 3 7 3 8 3 9 3 10 wr is 6 AM pH 21 21 21 22 22 24 24 25 26 28 27 30 1 1 2
124. 254 nm 312 365 nm DNA HL L 1 16
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