概要 必要な試薬と装置 Lyse/Washを用いた全血の直接蛍光染色
Contents
1. Vortex FACS Lysing Solution 10x BD 349202 FACS 0 196 PBS Dulbecco s PBS pH7 2 0 2 PBS 0 2um 8C2. 4 1 XPBS 98 5 IL 1 Clinical Applications of Flow Cytometry Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes Tentative Guideline Villanova PA National Committee for Clinical Laboratory Standards 1992 NCCLS document H42 T 2 Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture Approved Standard Villanova PA National Committee for Clinical Laboratory Standards 1991 NCCLS document H3 A3 3 Landay AL Muirhead KA Procedural guidelines for performing immunophenotyping by flow cytometry Clin Immunol Immunopath 1989 52 48 60
3. 3mL 500x g 5 196 PBS 0 5mL 2C 8C FACS Lyse Wash 1 EDTA BD FACS Lysing Salution BD FACS 3 FACS Lysing Solution
4. 35 8 0 19 PBS 19 8C 1 FACS
5. EDTAGK G0 85C 36 No ODMD 100 L 15x75mm G0 25C 15 30 10 FACS Lysing Solution 26mL 10 500x g 5
6. 4 Nicholson JKA Rao PE Calvelli T et al Artifactual staining of monoclonal antibodies in two color combinations is due to an immunoglobulin in the serum and plasma Cytometry 1994 18 140146 37
7. Lyse WWash FACS Lysing Solution 4 EDTA3K BD 367660 FALCON 126x75mm BD 352058 BD BD 34013290 BD
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