タンパク質解析の 2次元電気泳動を自動化
Contents
1. Auto2D 2 IEF pH4 7 PAGE 10 09 N AutO DD 0 02pH 2kDa 0 1pH N _ OO a oo 20 IEF 45 mr 1 2 3 3 mTOR mTOR2. 6 mm 240X 428X 445 536 17kg a AC100V 50 60Hz 160VA ds BM 1E 1 150 000 TFT 8 uu 4 I C Compact Flash Card P D 160GB 2 5inchHDD ee USB2 0X2 BM 1S 10 10 000 5 35C 35 859 RH IEF pH 4 7 3P 2P 1 BM 113010 20 000 pH 3 10 EF mm 60X 1 2X 23 PAGE BM 12075 30 000 7 5 1 20C 2 BM 12100 30 000 10 0 PAGE mm 70X 70X 12 2 4 2
3. 14 3 3 i _ w 2 TPA DMSO 200 nM TPA 12 O tetradecanoylphorbol 13 acetate 40zg Auto2D 2 PVDF 6 2D IEF pH3 10 PAGE 10 09 2 6 pHS3 pH10 pH3 pH10 Catenin PKD Catenin PTEN 544MAPK Re p44MAPK p
4. mTOR A Auto2D eIEF pH4 7 PAGE 10 09 mTOR mT0R pH4 ja 4 pH4 ya 4 Green Control cells Green Rapamycin treated cells Red Rapamycin treated cells Red mTORinhibitor A treated cells 14 3 3 _ _ Auto2D A Auto2D B pH4 5 5 IEF 2 2 eIEF pH4 5 5 A B PAGE 10 09
5. 30 5 1 1 Auto5D 1 10 all 5 3 1 1 M 1 SER 1 3 40 STA 30 SARS 20 n 5 4 0 RE CV BB 11 3 0 0gph Auto D 4 Auto2D 70xX70xX 1mm 3 58x48x 1mm 49
6. 42MAPK i p42MAPK DMSO TPA sw JST Tr581 8581 4 1 39 TEL 072 991 0681 URL http www sharp co jp sms 2012 6
7. SHARP BM 100 100 0 02pH PkDa _ TP i i TTP 2 Auto2D we mm 5 000 000 Auto2D Auto2D 4 1 a lt 1 2 MES
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