DNAプローブ「FR」MACダイレクト
Contents
1. 2 3 2 5 4 2 5 52. 0 966 2 8 32 0 988 1 166 0 985 _ 4 1 EE ga 0 Wen 9 20 gu 0 1 0 984 0 1 0 0 1 2 1
3. 2 20 x L 2 0 0 mk L 3 ND 1 5 1 5 15 3 TMA Transcription Mediated Amplification 9 5 1 5 1 2X75mm 5 0
4. MAC MAC MAC MAC MAC RNA rRNA RNA 3 4 MAC 1 1 1 00 000RLU 2 1 0 0 5 0 0 0 0 0RLU 2 1
5. 2 3 1 a Hs FE 1 hw 2 2 1 0 2
6. 2 1 1 1 3 mL 1 1 5mL 3
7. DNA R NA RNA DNA 50 1 2 2 0 2 SRE il RNA rm mL ATP 400nmol CTP 400nmol 6 GTP 400nmol UTP 400nmol PRT AE RNA DNA dATP 100nmol dCTP 100nmol OHNM dGTP 100nmol dTTP 100nmol T T7 15pmol 15pmol
8. N DNA 100 1000 4 5 RNA 6 0 Ressm AS TF 1 TMA PH 2 HPA Hybridization Protection Assay 8 2 GN in
9. Ds 50TMA Ds 50HPA 3 SO CY TMA Transcription Mediated Amplification Ds 5 0TMA Ds 5 0TMA 12X75mm 2 5 Ds 5 0 TMA 5 0 1 0 0 9 5 5 1 5 4 2 1C 5
10. 2 1 0 0 SEN MI 3 1 3 2 1 0 0 3 4 9 20CFU as say Wavium intracellulare 2 3 2 5 1 0 1 5 4 CFU mL WM tuberculosis H37Rv ATCC27294 MM tuberculosis H37Ra ATCC25177 UM africanum ATCC25420 MM bovis ATCC19420 M microti KK14 01 MM kansasii ATCC12478 M simiae ATCC25275 Ml marinum ATCC927 M serofulaceum ATCC19981 MM szulgai ATCC19530 M gordonae ATCC14470 MM asiaticum KK24 01 M terrae ATCC15755 MM nonchromogenicum ATC
11. 2 5 5 0 1 0 0 9 5 5 1 5 4 2 1 5 2 5 x 4 2 1 6 0 1 0 0 7 6 0 1 C 1 5 3 0 0 7 6 0 1 1 5 1 0
12. HIV HBV HCV 2 3 1 2 1 0 2 3
13. 4 C 4 6 2 0 C 6 4 C 2 0 C 4 HP A Hybridization Protection Assay 3 1 007L 3 3 6 0 1 1 5 3 0 0 83 3 6 0 1 1 5
14. 2 1 0 C 4 2 3 2 1 0 2 8 4 6 0 1 2 1 0 2 5 I 5 05 0 i 5 1
15. 0 067mo1 pH6 8 1mL 1 pH pH 4 9 2 MG I T 2 0 067mo 1 pH6 8 1mL 1 2 3 10 1 5mL 3 000Xg 20 1mL 0 067mo1l pH6 8
16. 15 SS 2 5 1 5 4 1 8 2 1 0 2 8 1 2 1 50 1 C Abe et al Detection of Mycobacterium tuberculosis in Clinical Specimens by Polymerase Chain Reaction and Gen Probe Amplified Mycobacterium Tuberculosis Direct Test Microbio1 31 3270 3274 1993 Gln 2 DNA Mycobacterium tuberculosis complex Mycecobacterium avium complex 36 2 197 200 1992 3 60 51 1985 4 24 214 220
17. RLU Relative Light Unit 20 gL 200 gL 95 15 25 Ds 50TMA TMA HPA Ds 50HPA 4 1 0 0 0 0 0RLU 2 0 0 0 0 0RLU 100 000RLU 200 000RLU 1 2 200 000RLU A DR
18. 2 MAC a NE EG NC PA 4 NALC NaOH N AL C N a OH p H Nye gt 7 4 HM avi 5 Mycobacterium avium complex Mavirum WM intracellulare MAC RNA r RNA RNNA
19. EE El 5 FE 1 i EE 1 5mL rm MMLV 2 500U T7RNA 2 000U mr MAC 1 1 0 X107 RLU 9 MAC 2 3 3 X 106 RLU 9 MAC 3 3 3 X 106 RLU A 55 ERE DNA a I EA 9 12 7 1 1 1 1 mL 400pg 8X1 0 4ce 1 Mycobacterium avium complex MAC rRNA 0 avium intracel7zilare rRNA 2 0 0 p g HH F
20. 1 5 1 0 1 50 5 0 i 2 RLU Relative Light Unit 5 BO CH TMA Transcription Mediated Amplifcation HP A Hybridization Protection Assay T 12X75mm
21. 1 2 0 2 0 0 7 3 9 5 5C 1 5 1 5 9 5 5C 1 5 9 5 5 C 5 9 5 5 C
22. RI 4 MAC 7 0 80 5 MAC MAC 4 8
23. Ds 50HPA 3 1 EC 1 5 mL NALC Na OH 2 2 0 1 0 0 067mo 1 pH 6 8 1 0m 3 000g 15 WV 2
24. 1 0 1 5 05 0 i 2 R LU Relative Light Unit 20 x L 200 gL 25 u 50 nL 100 gL 60C 15 10 3 2 1 2 3 1
25. 2 5 4 2 1 6 0 6 0 4 C 4 6 6 2 0C 6 4 C 2 0 C 4 HP A Hybridization Protection Assay Ds 50HPA Ds 50HPA 1 0 0 kL 6 1 1 1 5 3 0 0 7 6 1 1
26. 100r L OO SE 2 5 x L 9 5 5 1 5 2 0 4 2 1 G 5 4 2 2 57L 4 2 1 6 0 1 2 6 0
27. 1 6 mL 4 to 2 NALC NaOH 2 NaOH 1 45 Na citrate 0 5 N acetyl L cysteine 15mL 1510J 42 kHz 90W 11 2X75mm 2 5 200p 2 POP oO oO 50 50 i oO TT ETE EZ DC ECT A 1 2mm 9 5 Ks EEE CT EE 207 25 50L 100 2007L 300pzL Ds 50TMA Ds 50HPA Ds 5 0TMA
28. 4 1 2 1 2 0 2 5 9 el CO 2 5
29. 1999 1 HH 26 1 54 58 2000 TMA HPA Mycobacterium avium Complex 29 1 93 100 2002 2 62 5 TEL 03 5695 9210 FAX 03 5695 9234 2 62 5
30. 1 TMA Transcription Mediated Amplification R NA RNA RB DNA RNA T 7 KSO2T 2006 4 1 RNA RT DNA cDNA RT RNase R NA cDNA 21400AMZ00062000 RT DNA DNA RNA RNA Po1 RNA RNA DNA FR MAC DNA R
31. C19530 M xenopi ATCC19250 MH gastri ATCC15754 triviale ATCC23292 MM ulcecrans ATCC19423 M haemophilum ATCC29548 Ml malmoense ATCC29571 fortuitum ATCC6841 WM abscessus ATCC19977 M chelonae ATCC35752 MH favescens ATCC14474 vaccae ATCC15483 5S pneumonzae C a7bicans co77 pneumoniae Proteus sp aureus infuenzae _ aeruginosa L pneumophila ATCC33152 marcescens 2 0 1 2 3 9 2 3 4 1 1 0 0 1 0 1 2 2 2 1 7 9 8 5 1 00 9 9 0 1 0 0 100 000RLU 2 0 0 0 0 0 RLU
32. NA 1 T7 TI mB gt 1 6 2 WhNeseh Ei 3 T7 mmmmmmmmmmmmm DNA 4
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