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1. 5 kb 4 PCR 10 1 4 M 1 kb Ladder Marker 5 10units 37C 1hFr 1 6 6 PCRH RFLP 4 PCH N A KOD FX DNA2. A N A X g 1 Dam 9 A DH5o A 8 BACT HG A
3. kssx 10ml 5ml 1 0mIl HepG 5 6
4. siRNA J siRNA MASS siRNA UPLOAD Vol 88 siRNA gE 30 1
5. 1 a Oct3 4 RT PCR Primer 2 EN 2 EN SASCb279 SASC361823 SASCG61 4 SASCG612GPRH SASCG61 4PR SOx SASC 1 7320 SASCG8408 SASCG8409 SASCG8408PR SASCG8409dPR Nanog SASC3033 1 SASC303LY SASC43958 SASC44833 SASC43958PR SASC44833PR E Cadherin SASC 870 SASC35b242 AG 49 AG CG 4 PR SASCG5 48PR TRA 1 60 SASC 1705 TRA 1 8 1 SASC 1706 Sall4 SASC46045 SASC45808 SASC45809 SASC45808PR SASC45808PR TERT SASC 1 SASC540380 SASCG664 1 SASC106829 SASCG664 SASCG6641PRH SASCG664 PR LIN28 SASC106829PR ssEA1 SAsce12709 ssEAr3 SAsce1709 hh SsEA 4 SsAsc58368 Wh 4 C S6 00 49 000 siRNA 20C 55 00 47 000 RT PCR Primer 20C 5 000 Santa Cruz Biotechnology 4 2 13 Santa Cruz Biotechnology 4
6. PCR PCR 1 x 1 10 100 5 ru PCR condition Template x1 x10 xX100 5 ul 50 ul Reaction Target Mouse membrane glycoprotein Thy 1 gene 2 6 kb Accession No M 10246 Primer 1 5 CCACAGAATCCAAGTCGGAACTCTTG 3 26mer Primer 2 5 GTAGCAGTGGTGGTATTATACATGGTG 3 27mer PCR Tm 73C 2xPCR buffer for KODFX 25 ul 3 2 mM dNTPs 10 ul 94C 2 min 10 pmol ul Primer 1 1 5 Ll 98C 10 sec so a 10 pmol ul Primer 2 1 5 ul 68C 2 5 min 94C 2 min 5 98C 10 sec KOD FX 1 0 U l laul Tm 5 C 30 seo lo CyCles Autoclaved distilledwater Xl 68C 1 min kb Total 50 ul min kb Taq
7. HepG Cos 1 Huh7 HeLa co 222HI MoE NG 2 0 SIGG 7 www toyobo co jp bio PCR
8. www toyobo co jp bio MultiReporter Assay System TrIDIuC MultiReporter Assay System Tripluc 6 lt ay O Emerald Luc pDELuc 2 Emerald Luc Vector Code No ELV 101 ELV 201 Emerald Luc Assay Reagent Code No ELA 101 ELA 102 ELA 201 D Luciferin Code No MRL 101 MRL 101X5 008 6 1SO 9001
9. 3 mm 50 mM NaOH 180 gl Vortex 95C 10 min 1M Tris HCI pH8 0 20 gl Vortex 12 000 rpm 10 min 0 5 2 gl PCR 50 gl 2 KOD Plus PCR KOD Plus Ver 2 5 KOD FX Taq DNA polymerase 11 PCH TA TArget Clone Plus Code No TAK 201 TA KOD Plus KOD Plus Ver 2 Taq 80 KOD FX KOD FX 5 KOD Plus KOD Plu
10. KOD_FX Primer R 5 ACATGTATTTGCATGGAAAACAACTC 3 globin 8 5 kb Primer F 5 TGATAGGCACTGACTCTCTGTCCCTTGGGCTGTTT 3 Primer R 5 ACATGATTAGCAAAAGGGCCTAGCTTGGACTCAGA 3 15a _ 13 8 5 kbD 15b PCR Q amp A KOD FX a B C Long _ A PCR PCR OD FX lt TagqDNAPol KN Taq Taq 99 0 M 1 2 3 M 1 2 3 M 1 2 3 M A 1 3 kb KOD EX 7 lt SamDple gt D KOD FX 1 Whole blood 1 4 M1 A B CM2 2 Whole blood 2 gl 8 5 kb 3 Whole blood 4 gdl 3 6 kb a 13kD 3 6 kb M
11. E 2 50 Human 7 globin cluster M1 1 kb Ladder Marker 1 1 3 kb 2 3 6 kb 3 8 5 kb M2 A Hind I digest 1 PCR KOD FX 1 3 mm Proteinase K 50 mM NaOH 180 ul Vortex 1 95C 10 min 1M Tris HCI pH 8 0 20 Vortex 12 000 rpm 10 min
12. Stop Xbal 5 GATGGCCACGGCTT6ATCTAGATCC 3 3 CTACCGGT6CC6AACTAGATCTAGG 5 NN Dam A DNA 5 GAGCAAGAGATGGCCACGGCTHGCTICCAGCTCCTCCCT lt CAAGAGATGGCCACGGCTTGATTCTAGACC df BACT R2 Stop Xbal BACT F 5 GCTCCATATGGATGATGATATCGC 3 BACT R2 5 GGTCTAGAATCAAGCCGTGGCCATCTCTTG 3 9 A A 10 A PCR
13. A N 2 NdeIl Xbal A Human 8 Actin CDNA C cDNA N TA Human G3PDH cDNA C 1 2 cDNA F Mco 1 R 796 TGA XDa 1 PCR PCR KOD Plus Nde I a Nde aa phnsa 2 F Mge 1 R 732 TGA Xpa 1 Ncol Xbal co1 A 1 co1 3 p pHse X g
14. TA 8 A N cDNA PCR KOD Plus or KOD Plus Ver 2 KOD FX Vol 1 2 PCR Vol 2 4 Vol 5 1 PCR Vol 5 2 4 TA TArget Clone Plus Vol 5 6 Nde I or Nco 1 Xa 1 PCR Vol 1 7 Vol 2 7 Vol 5 5 8 A N PCH
15. PCR KOD FX PCH KOD FX KOD FX 1 00UX1 200 KFX 101 35 000 KOD FX 1U Ri XPCR Buffer for KUD FX i 8 mM dNTPs 200Ux1 x5 1 000 KFX 101X5 140 000 0 KOD FX DINA TA TArget Clone Plus TA 6 TArget Clone Plus 10 TAK 201 16 000 9 I gW 1 m 1 LTTT WD
16. FF GEHE ID LL 2 LT Tr 1 Tp13 1 1 ETFS lrpis i O17 1 17p13 1 1 ET 1 TT iT7Tpls 1 _ Antibaodies it Fatalo aD TE Fumection OI 1 D1 ET Lu iT LT FF TT fe LE i UPLOAD 15 HOT a C DNA ws s2oo87Aag 2o08FefesH cDNA CDNA Total RNA 1 cDNA PCR
17. Jurkat 6x104cells 6 1 3 8 5 kb 14 CO PCR EDTA PCR KOD ITTTTTTTTTHTHTTTTTTTTTHTTTTTTTTTTTHHI Tm 73C 3 globin 1 3 kb FX 1 1 3 kb A PCR Primer F 5 TTAGGCCTTAGCGGGCTTAGAC 3 Primer R 5 CCAGGATTTTTGATGGGACACG 3 globin 3 6 kb 4 gl KOD FX Primer F 5 GGTGTTCCCTTGATGTAGCACA 3
18. gt http www toyobo co jp Seihin xr lifescience tech reader contribute index html 10 000 2 000 17 Santa Cruz Biotechnology 008 7 2 Santa Cruz Biotechnology 10 15 SiIRNA PROTEIOS PROTEIOSW Wheat germ celLfree protein synthesis kit ver 2 PROTEIOS W CPS 801M 5 008 9 CPS 801 0 CPS 803 60 CPS 0 PROTEIOS Buffer Set
19. 3 TESTSKIN LSE melano 3 3 s i a AM 4 1 3 TESTSKINTV LSE melano 6 BL 3 HE 14
20. gt yx 2 S KOD FX KOD FX PCR 50 ul 5 2 lI Proteinase K KOD FX
21. 6 6 1 1 LSE 101 110mLX 3 3 TESTSKIN LSE melano 392 1 1 60l 10 14 PBS 1 4 405nm Control PBS 1
22. PCR Nde I Xbal Nde I Xbal a A NdeI Xbal yw PCR Nde I Xbal NdeI Xbal lL SA HT 10 A 2008 2009 p3 41 PCR PCR RFLP EF N N A N G Xba 1
23. 2XPCR buffer for KOD FX 2mM dNTPs 0 4 mM each KOD FX PCR PCR 10pmoll Primer 1 10pmol gl Primer 2 0 3 uM 0 3 uM GC Template DNA Plasmid DNA 50 ng 5044dl PCR Taq 11 cDNA 200 ng RNA 50 2 ll 1 0 U 50 gil DNA 200 ng 50 gl PCR grade water KOD FX 1 0 U l 1 Total 50 DNA PCR 2 3 PCR PCR 13 2 min 10 sec 68 C
24. CLIP MEK1 H2B SNAP CLIP MEK1 H2B SNAP 6 CLIP tag SNAP tag A Gautier et al Chemistry amp Biology 15 128 136 2008 CT TMR CT 505 CLIP cell Block pCEMS1 H2B CLIP10m control plasmid CLIP cell 360 Starter Kit CLIP cell 430 Starter Kit Ex357nm 437nm DAPI filter Ex421nm Em444 487nm Blue laser filter cvLk3s39 25 000 cvsk326 g5 0oO CVSKG 8 95 000 CLIP cell 505 Starter Kit Ex504nm Em532nm fluorescein filter CVSK332 95 000 CLIP cell PF Starter Kit Ex500nm Em 532nm fluorescein filter CVSK330 95 000 CLIP cell TMR Starter Kit Ex554nm Em580nm rhodamine filter CVSK334 95 000 CLIP tag Cell Surface Trial Kit CLIP block cell CT 488 CT 547 pCEMS1 CLIP10m NK1R control plasmid CVLKG4Z 44 000 CVBKG40 35 000 CLIP Diotin 100 Kit CVLKG386 66 000 CLIP vitro 488 50 Kit Ex506nm Em526nm flurorescein filter CVLKG4 1 95 000 CLIP vitro 547 50 Kit Ex554nm Em568nm TAMRA or C3 filter CVLK843 95 000 CLIP vitro 647 50 Kit CLIP vista Green 50 Kit Ex660nm Em673nnm 650 nm diode laser Ex500nm Em524nnm 488nm fluorescence sensor CVLK845
25. DNA 3 7d g 5 NNNN OH 3 3 NNNN 0H 5 3 PCR HO NNNN 3 HO NNNN 5 PCR 5 NNNN OH 3 5 HO NNNN 3 SM 5 3 NN PCR PCR 7 DNA DNA 14 DNA Ligase T4 DNA Ligase 5 NNNN e 5 NW OH 5 HO INMMNMI3 14 DNA Ligase 5 INMMLT NNNNI3 5 INNNN_THNNM3 3 NNNN CP 5 3 HO IA NNNNI5 3 NNNN HA NNNMI5 3 NNNN HA NNNNI5 T PCR A 14 DNA polymerase or 6 5 5 KOD DNA
26. TESTSKINTM TESTLIVERTM KOD FX PCR KOD FX PCHPH TKOD DNA Polymerase PCR PCR PCR PCH PCR GC KOD FX PCR 1 KOD FX PCR
27. 3 pCEMS 1 CLIP10m CLIP tag N C SNAP Tag CMV CLIP tag codon usage pCEMS 1 H2B CLIP10m pCEMS 1 CLIP10m NK1R 14 UPLUIIH Santa Cruz Biotechnology SANTA CRU Q BIOTECHNOLOGY Santa Cruz Biotechnology 10 SiIRNA RT PCRH mRNA Santa Curz Biotechnology
28. Lloyd s Register Quality Assurance LRQA ISO 900 1 Restriction Endonucleases Modifying Enzymes NOTICE TO PURCHASER LIMITED LICENSE PCH www toyobo co jp bio QS Accreditation R 016 IS0 14001 JAB LA_ JIC Quality Assurance Ltd IS01400 1 a Ag e
29. DNA JM 109 DH5Z Dam Dcm Dam GATC N6 Dcm CCWGG W A or T C5 4 Dam GATC Mbo I GATC Dam Bc 1 TG
30. EcoRV SphI Eco RV Pst I EcoRV SphI EcoRV PstIl SphI 5 AGGAGGTGAT C ORF 3 5 AGGAGGTGAT G CAIGIC ORF Sl 3 TCCTCCACTA GTAC G 5 3 TCCTCCACTA ACGT CIGTAC G 5 SD SD 6 Bam HI Bam HI KOD DNA Polymerase SOD DNA Re MAASee 0 EcoRV EcoRV Sp 1 5 AGGAGGTGAT CATGC ORF 3 5 AGGAGGTGAT G CATGIC ORF 3 TCCTCCACTA GTACG 5 3 TCCTCCACTA CIGTAC G 5 SD SD Bam HI Bam HI 17 A 18 N S 8 8S PCR T4 Polynucleaotide kinase
31. 3 N PCR G3PDH http www toyobo co jp bio S A N S 4 3 A PCR pm 7 DNA
32. Buffer Compatible site O 5 A GATCT 2 G AATTC 1 GAT ATC Blunt Site A AGCTT Ncol GGTAC C C CATGG c C GGCCGC D Not I G Pst I CTGCA G Sg 1 G TCGAC D A CTAGT T CTAGA Dam OO PO OO PP OO O Dam Dcm PCRH C TCGAG O
33. N A N A N N 1 G3PDH R Xpg 1 XDg 1 Nco I Nde 1 ATG DNA Mge 1
34. ReverTra Ace dT gt lt PCR gt nm ACE anglotensin onan enzy me DW 0 erg SYBR Green Realime PCR Master Mix 5 Code No QPK 201 LB actin 7 od Primer F 10M 0 4 Primer R 10M 0 4 cDNA 2 200 10 ul 1 SYBR Green Bioflux LineGene Code No BFFQD 33A DCPN PC101 20 000 DCPN KD101 20 000 CDNA 1 DCPN MS101 0 000 DCPN HT101 20 000 DCPN HC101 20 000 DCPD PC101 20 000 DCPD KD101 20 000 DCPD MS101 20 000 cDNA DCPD HT101 20 000 DCPD KDe01 20 000 DCPD CC101 20 000
35. PCR PCR 2008 mMl 3 TOYOBO A PCR B Long PCR KOD FX Taq Taq M 1 X10 X100 M X1 X10 X100 M M X1 X10 X100 M n 2 M 1 kb Ladder Marker 3 PCR PCR 1 5 ul 3 KOD FX PCR 6 kb PCR DNA Proteinase K PCR KOD FX KOD FX
36. RR 4 DNA DNA me20 SUR KG EL UI Xoa1 6So xpai CTGCCG AAAACCTGCCAAATGAGATTCTAGAAAGAAGG 3 3 GACGGC TTTTGGACGGTTTACTCTCAGATCTTTCTTCC 5 N DNA ORF Xpa1 Xbal 796 5 CIGCCGTCTAGAAAAACCTGCCAAAIAIGATGACATCAAGAA CCGTCTAGCAAAACCTGCCAAATGAGATTCTAGA G3PDH R2 Stop XpaI G3PDH F 5 ACCCATGGGGAAGGTGAAGGT 3 G3PDH R2 5 TCTAGAATCTCATTTGGCAGGTTTTGCTAGACGG 3 12 N N 0RF X g 1 12 N Dam DNA
37. MoE E02 I Sn IS014001 A 18
38. OSNAP tag benzylguanine BG SNAP tag CLIP tag N CT CLIP cell CLIP vitro vista CLIP Biotin 1 SNAP tag CLIP tag SNAP cgll CLIP cell Kit CLIP tag Duilding block SNAP tag 2 CLIP tag SNAP tag N CLIP tag MEK 1 CLIP MEK 1 C SGNAP tag histone HB H B SNAP CHO CT TMR CLIP cell TMR Kit BG 505 SNAP cell 505Kit 30
39. PCR Ba HI T4 Polynucleotide Kinase DNA MS 5 3 DNA polymerase 3 5 Exonuclease e EcoRV Pgr TI Sph1I Pg77HI A N KOD DNA polymerase 5 3 DNA polymerase 3 5 Exonuclease DNA A 5 DNA N 1 7 18 SD 5 6
40. N Dam GATC Xba 1 1 XDg I GATC N S 7 11 Stop Xbal ACCTGCCAAATGAGATTCTAGAAAGAAGG 3 3 TGGACGGTTTACTCTAAGATCTTT TTCC 5 N DNA 1 9 ACCTGCCAAATGATCTAGAATCAAGAAGG TGGACGGTTTACTAGATCTTAGTICTTCC ACCTGCCAAATGAGICTAGATCAAGAAGG Dam TGGACGGTTTACICAGAICTAGTTCTTCC ACCTGCCAAATGAGATCTAGACAAGAAGG TGGACGGTTTACTCTAGATCTGTTCTTCC dl 11 N N
41. A 100 80 B 80 50 C 50 20 D 20 0 1 2 4 2 3 lt gt Biotechniques 19 56 59 1995 EE 2a A N T S 5 http Www toyobo co jp Dio E A cDNA PCR KOD Plus 6
42. lb Y a Nip J wm mahi gt D I A HC HC HC HC HOb HOb HOb Total Kit HOb Total Kit HFLS HFLS OA HFLS RA HFLS Total Kit HFLS OA Total Kit HFLS RA Total Kit 1 Total Kit 1vial A CA090K 1vial 5X105cells
43. 5 3 6 a Xba I Aat I AGGCCT Banh ATCGAT 2008 2009 9 41 jp GATC gt ke Dam 5 CCAGG BraCCTGG Dcm 3 GGTCC OM 4 Dam Dcm GA TCTAGA CT IAGATCT w X m TCTAGA TC AGATCT AG Xba1 Dam 5 Xba I T14 DNA
44. 2008 9 65 CDNA www toyobo co jp Dio 1 Total RNA PCR PCR
45. 14 4 gt 4 16 5 1 4 12 al 6 1 1 4 i a x 0 0 14 14 5 14 3 709 5 2008 11 14 TESTSKIN LSE melano TMLSE 101 75 000 lt 6 gt LSE 101 lt P20m 4C
46. 95 000 CVLK349 5 000 CT GLA NHS CLIP tag building block CVBB353 52 000 CT NHla CLIP tag building block CVBB3 561 52 000 CLIP source plasmid pCLIP10m CLIP tag CVPLG 9 27 000 CLIP express Mammalian Stable Expression Plasmid Kit CVPLG 4 52 000 4 3 DMSO www covalys com SNAP tag www toyobo co jp bio CLIP tag Covalys
47. ATP DNA 5 6 PCR 1 5 NNNN OH 3 5 WINNWS 3 NNNN OD 5 3 HO NNNNS 6 14 DNA Ligase 1 Ka PCR 5 DNA a 7D c TA PCR TA
48. 2 5 3 3 5 Exonuclease 12 R 3 3 4 5 KOD FX 2 PCR PCR PCR PCR KOD FX
49. CA411500 CA411D250 CA414500 CA415500 CA416500 CA417500 CA417D250 30 OFF 2008 9 1 2008 1 1 28 109 000 111 000 141 000 144 000 110 000 114 000 145 000 146 000 131 000 131 000 131 000 169 000 169 000 169 000 13 000 23 000 20 000 13 000 26 000 13 000 26 000 15 000 76 300 77 700 98 700 100 800 77 000 79 800 101 500 102 200 91 700 91 700 91 700 118 300 118 300 118 300 9 100 16 100 14 000 9 100 18 200 9 100 18 200 10 500 CIENCE SERIES PCR ku PCR PCR S
50. polymerase d 5 NNNNNNNN 3 o NNNNN 3 5 NNNN 3 5 NNNNNNNNN 3 3 NNNN 5 3 NNNNNNNNB5 3 NNNNPD5 3 NNNNNNNNNPD5 P x NNNN 3 MI 3 NNNNHNNNNI5 w 3 NNNNHNNNN 5 PCR PCR PCR 1 DNA 1 1 G GATCC
51. 0ulXX1 FSK 101 LGK 201 MagExtractor PCR amp Gel Clean up DNA NPK 601 PNK 111 22 000 28 000 200 1 500UX1 T4 Polynucleotide kinase DNA rATP E coli Alkaline Phosphatase 15 000 50umoles 0 5ml ATP 111 15 000 100UX1 BAP 111 MagExtractor Plasmid 500 NPK 301 1 MGS 101 15 000 33 000 10 TAK 101 38 000 16 000 17 000 29 000 Magical Trapper TArget Clone TA Cloning vector 10 TAK 201 0 1mlX10 DNA 900 0 1mlX10 DNA 903 0 1mlX20 DNA 913 TArget Clone Plus KOD Competent high JM109 Competent high DH5c Competent Quick DH5o CLIP tag SNAP tag CLIP tag SNAP tag Human 08 alkylguanine DNA alkyItransferase hAGT G0kDa benzylcytosine CT
52. 1 1 kbLadder Marker n 2 13kb M2 A Hind I Marker lt Sample gt Jurkat 2X104cells 14 DNA _ lt SamDle gt Whole blood 2 gl 15 DNA EW S 5S SD Shine Dalgarno SD ATG 5 6 Eco RV PsfI SphI ES 5 AGGAGGTGATIATC C T6CAl6 CATGIC 3 e 2 TGCTCCACTAITAG G RCeT ClerA6 e ORF pg SD KOD DNA polymerase T4 DNA
53. A EDTA 100ml 2 A 20C 3 HIV HBV HCV CELL APPLICATIONS INC Total RNA http Www cellapplications Com 1 Y Wada et al Int Immunopharmacol 6 144 55 2006 2 T Ando et al Arthritis Res Ther 8 R146 G006 3 J Quintavalla et al J Cell Physiol 204 560 6 2005 4 H sS Cha et al Arthritis Rheum 54 587 92 2006 5 J Luetal J Infect Dis 193 582 90 2006 adult fetal adult fetal adult fetal adult fetal adult adult adult adult adult adult Code No CA40205a CA40205f CA402K05a CA402K05f CA40605a CA40605f CA406K05a CA406K05f CA40805a CA408OA05a CA408RA05a CA408K05a CA408OAK05a CA408RAK05a CA410500
54. ATCA 100 Dcm CCWGG EcoR II Xba I Xba 1 TCTAGA GA TC Dam DNA 5 3 DNA Vc I
55. COPD DCPD LG101 20 000 2 2 ME 0 035mM 1 1 1 ul 20ng Total RNA cDNA COPD www toyobo co jp bio 16
56. UPLUHI 1 0 Y 0 B 0 C HH EE MM C A 1 S d 7006 August MWL TESTSKIN LSE melano p 1 Er amnd Neu 1tem 1 TESTSKIN LSE melano 3 TECHNICAL REVIEW 3 KOD FX PCR CAMPAIGN 5 CELL APPLICATIONS INC 309 2OFF 6 PCR 2 NEW RELEASE 14 CLIP tag HOT ITEM 15 Santa Cruz Biotechnology 16 OO DNA 17 17 7 INFORMATION 18 Santa Cruz Biotechnology 18 PROTEIOS 18 MultiReporter Assay System Tripluc 18 Emerald Luc pELuc ESTSKIN LSE melano GO0B 7 4 008 1 1 14
57. a 1 Stop Xpa 1 TGA PCR PCR KOD Plus KOD TA TArget Clone Plus Code No TAK 201 2 3 A N PCR Dam GATC Xba I ORF Mael GAGCAAGAGATGGCCACGGCTGCTTCCAGCTCCTCCCTGGAGAAGAGCTA E QQ EMA A A S SM S S L E K S GATGGCCACGGCTIGATCTAGATCC 5 lt STOP 2g1 BACT R 2 A PCR actin 5 GCTCCATATGGATGATGATATCGC 3 BACT F Mcal CGCCGCCAGCTCACCATGGATGATGATATCGCCGCGCTCGTCGTC 1 M D DD IA A L V V 5 ACCdATGGGGAAGGTGAAGGT 3 G3PDH F TCGCTCAGAACACCTATGGGGAAGGTGAAGGTCGGAGTCAAC MGKVKV GV N CTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAGGTG C R L E KP A K YD D I KK 3 ACCTGCCAAATGAGATTCTAGAAAGAAGG STOP 4 gl G3PDH R
58. s Ver 2 Tm 5 10C 2 Tm 73C 2 PCR 10kb gt 10kb HPLC 30 000 Code No KOD 201 KOD 211 200UX1 200UX1 KOD FX PCR KFX 101 LDP 101 200UX1 250UX1 KOD Dash Blend Taq PCR Blend Tag Plus Hot start 35 000 250UX1 19 000 250UX1 BTQ 101 BTQ 201 ReverTra Ace a Ligation high Ver 2 Ligation 21 000 53 000 100 75
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