トマト 化葉巻病診断キット
Contents
1.2. 100 mM Tris HCI pH 8 0
3. 1 Ei LAMP DNA TYLCV
4. TYLCV TYLCV LAMP TYLOV
5. 13 1 x 3 63C 60 14 60 80 C 2
6. 1 1
7. 1 1 gt ii 100000 ppm 1 ii
8. MSDS http nippongene analysis com 8 9 LAMP LAMP 10 e Genome Order
9. 4 5 6 TYLCV 7
10. UV gt
11. e Genome Order http genome e mp jp 6 7 RO LAMP Loop mediated Isothermal Amplification NN SE O PF Yi Ra rR RC NN SNC CE RR RR RR RNR 5 FPPLTFTFTPHTPTE7 IPPTF TFPTFFYTT 1 TT TK 17 RT 18 1 LAMP TYLCV Tomato_ yellow leaf cu virus
12. 5 UV a UV 240 260 nm 350 370 nm 320 nm NA Eo 5 8 UV 60
13. NE4111 4 7 1 1 25 1 50 1 46 ul 234 6 ul 02n 0 2 ul 5 0 ul 130 0 ul 255 0 1l 1 2 1 5 0 ul 5 0 ul 3 Qs 4 7 5 lil 10 0
14. DNA LAMP Eiken GENOME SITE http Iloopamp eiken cojp amp amp ISO 13485 2003
15. 2009 B Q ELISA Enzyme Linked Immunosorbent Assay PCR Polymerase Chain Reaction ELISA PCR DNA POCR 2 DNA LAMP Loop mediated Isothermal Amplification LAMP DNA
16. TYLCV TYLCV DNA 1 MSDS 3 0 5 10 ul 10 10
17. 70 iv 1 v 10 TYLCV TYLCV TYLCV 20 C 1 x 3 1 1 1 5 ml 2 0 ml
18. DNA 1 C EDTA D A
19. 1 x 3 1 1 5 ml 1 1 TYLCV 12
20. A A 240 260 nm 350 370 nm UV 320 nm 16 6 1 Fukuta S Kato S Yoshida K Mizukami Y Ishida A Ueda J Kanbe M Ishimoto Y 2003 Detection of tomato yellow leaf curl virus by loop mediated isothermal amplification reaction J Virol Methods 112 1 2 35 2 2003 LAMP 112 1 2 35 3 2005
21. DNA DNA DNA 1990 DNA DNA
22. http www nippongenematerial com 2 50 NE0011 50 Me FE ME 20C 20C 20C TYLCV 20C 20C 50 50 50 20 C 6 20 C
23. 5 0 ul 10 0 ul i 10 0 ul i 10 0 ul ii TYLCV 1 0 ul 10 0 ul
24. Ioop mediated isothermal amplification LAMP 4 Notomi T Okayama H Masubuchi_H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA c e c Acids Res 28 12 e63 5 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 1 7 7 TYLCV 1 0 ul 5 0 ul 1 DNA 63 C 1 LAMP 4 6 TYLCV Tomato yellow leaf curl virus DNA GenBank Accession No AB014347 AB116633 AB014346 AB110218 AB116632 AB116635 AB116636 AB116634 AB110217 AB116630 AB116629 AB116631 AB192965 AB192966 AY530931 NC_004005 AJ489258 AJ223505 AF024715 AY5941 74 AJ132711 AF1059 7 AY134494 AJ865337
25. L i i 1 A TYLCV Detection Kit VeFSIOn 7 0 0 NEO0011 DD version 7 0 0 1 LAMP 2 2 6 3
26. 10 15 5 A A TYLCV TYLCV TYLOV B
27. LAMP DNA LAMP DNA TYLCV TYLCV 60 65C 60 LAMP DNA
28. AF071228 X61153 TYLCV Aichi TYLCV Atu TYLCV Shizuoka TYLCV lsrael Sz TYLCV SzY TYLCV SzD TYLCV SzOs TYLCV Kis TYLCV lsrael Ng TYLCV Omu TYLCV Miy TYLCV Mis TYLCV Tos TYLCV Tos H TYLCV Florida TYLCV Alm TYLCV Almeria TYLCV Cuba TYLCV Dominican TYLCV Egypt TYLCV Iran TYLCV Portugal TYLCV Puerto Rico TYLCV Reunion TYLCV Spain 7297 TYLCV Sardinia V 18 100 mM Tris HCI pH 8 0 100 mM 7 is HCI pH 8 0 50 0 500 0 ul 555 100 mM Tris HOI pH 8 0 A 100 mM Tris HCI pH 8 0 100u lt 100 mM Tris HOCI pH 8 0 10 0 ul 19 0 5N 0 5N 1 100 0 ul 50 mg
29. 6 6 MI 6 5 0 pl 100 mM Tris HCI pH 8 0 100 mM Tris HCI pH 8 0 495 0 pl 20 DD lt 2015 5 NIPPON GENE e TEL 076 451 6548 TEL 03 6712 3885 FAX 076 451 6547 FAX 03 6712 3886 E mail suppot nippongene analysis com E mail feast egenome csjp fujitsu com URL http n
30. ul 6 63C 60 7 80C 2 8 UV L 0 VV TYLCV TYLCV TYLCV 2 1
31. 0 ul 200 1 000 ul 1 5 ml 2 0 ml 60 65 C UV 240 260 nm 350 370 nm 1 5 ml 0 2 ml 100 mM Tris HCI pH 8 0 O0 5N LAMP
32. ippongene analysis com URL http genome e mDpJp Copyright 2015 NIPPON GENE CO LTD All Rights Reserved
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