トマト黄化葉巻病診断キット Ver.2 取扱説明書
Contents
1. V2_ 300_JA16FM Ss2. 100 mM Tris HCI pH 8 0
3. 10 _V2_ 300_ A16FM 15 5 A
4. TYLCV TYLCV LAMP DNA TYLCV
5. 1 1 gt i ii 100000 ppm 1 ii 70
6. 1 V2_ 300_JA16FM 14 2 2 UV gt
7. MSDS http nippongene analysis com 8 9 LAMP LAMP 10 e Genome Order e Genome Order http genome e mpp
8. A TYLCV TYLCV TYLCV B DNA 1 C EDTA
9. V2_ 300_JA16FM 16 6 1 Fukuta S Kato S Yoshida K Mizukami Y Ishida A Ueda J Kanbe M Ishimoto Y 2003 Detection of tomato yellow leaf curl virus by loop mediated isothermal amplification reaction J Virol Methods 112 1 2 35 2 2003 LAMP 112 1 2 35 3 2005 Ioop mediated isothermal amplification LAMP 4 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA c e c Acids Res 28 12 e63 5 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 V2_ 300_JA16FM 1 7 7 TYLCV 1 0 ul
10. CFS FS 1 V2_ 300_JA16FM LAMP DNA TYLCV DNA
11. DNA LAMP DNA TYLCV 60 65 C 1 LAMP DNA DNA DNA
12. NE0051 0 5 10 ul 10 100 ul 200 1 000 ul 60 65C V2_ 300_JA16FM UV 0 2 ml 240 260 nm 350 370 nm
13. gt 10 TYLCV 23 0 ul 115 0 ul 230 0 ul 100n 25 0 ul 125 0 ul 250 0 ul V2_ 300_JA16FM 1 1 3 1 2 1 4 25 0 ml 1 5
14. 63C 1 1 V2_ 300_JA16FM 13 2 2 1 UV 1 80C 2 UV UV 240 260 nm 350 370 nm 320 nm UV 19
15. LAMP Eiken GENOME SITE http Iloopamp eiken cojp amp amp ISO 13485 2003 http www n egt com V2 300 A16FM 2
16. B A 1 16 1 1 5 ml 50 500 ul 100 mM Tris HCI pH 8 0 2 50 mg 1 5 ml 0 5 N 100 ul 5 1 5 ml 495 100 mM Tris HCI pH 8 0 3 100 mM Tris HCI pH 8 0
17. DNA 1990 DNA DNA 2009
18. 2009 6 NIPPON GENE TEL 076 451 6548 TEL 03 5322 5424 FAX 076 451 6547 FAX 03 5908 1538 E mail SuDport nippongene analysis com E mail fsol egenome cs jp fujitsu com URL http nippongene analysis com URL http genome e mD jp Copyright C 2009 NIPPON GENE CO LTD All Rights Reserved
19. 1 5 ml 100 mM Tris HCI pH 8 0 0 5N V2_ 300_JA16FM 4 7 Se 1 TYLCV 23 ul 115 ul 230 Ll 1 2 1 25 ul 25 Ll 3 _V2_ 300_ A16FM 0 TYLGV 1
20. D A A A 240 260 nm 350 370 nm UV 320 nm A
21. iv 1 v V2_ 300_JA16FM 10 1 1 1 TYLCV TYLCV 20C 1 x3 1 5 ml 1 x 3
22. 4 5 6 TYLCV 7
23. B Q ELISA Enzyme Linked Immunosorbent Assay PCR Polymerase Chain Reaction ELISA PCR DNA PCR 2 DNA LAMP Loop mediated isothermal amplification LAMP DNA DNA
24. Ver 2 Version 3 0 0 NEO0021 DD Ver 2 version 3 0 0 Ver 2 1 2 20 C 6 3
25. X A Ox 1 1 amp _V2_ 300_ A16FM 63 C
26. 8 TYLCV 1 0 ul 20 0 pl V2_ 300_JA16FM 12 1 x 3
27. ea a 1 10 20 C 6 20 C TYLCV DNA V2_ 300_JA16FM 3
28. UV a 1 NN v V2_ 300_JA16FM TYLCV TYLCV TYLCV 2 1
29. 25 ul 1 DNA 63 C 1 LAMP 4 6 TYLCV Tomato yellow leaf curl virus DNA GenBank Accession No AB014347 TYLCV Aichi AB116633 TYLCV Atu AB014346 TYLCV Shizuoka AB110218 TYLCV lsrael Sz AB116632 TYLCV SzY AB116635 TYLCV SzD AB116636 TYLCV SzOs AB116634 TYLCV Kis AB110217 TYLCV lsrael Ng AB116630 TYLCV Omu AB116629 TYLCV Miy AB116631 TYLCV Mis AB192965 TYLCV Tos AB192966 TYLCV Tos H AY530931 TYLCV Florida NC 004005 TYLCV Alm AJ489258 TYLCV Almeria AJ223505 TYLCV Cuba AF024715 TYLCV Dominican AY594174 TYLCV Egypt AJ132711 TYLCV Iran AF105975 TYLCV Portugal AY134494 TYLCV Puerto Rico AJ865337 TYLCV Reunion AF071228 TYLCV Spain7297 X61153 TYLCV Sardinia V V2_ 300_J
30. _V2_ 300_ A16FM 1 1 Ver 2 LAMP Loop mediated isothermal amplification 2 NR NN CRS RR Ta 2 3 lll 3 4 NR NR N A 5 PPE 5 7 PH 1 1 5 NR i SR 14 6 NN EU CN a 15 7 RN A NA i i 4 16 V2_ 300_JA16FM 1 Ver 2 LAMP TYLCV 7oz azo yellow leaf curl_virus LAMP SARS
31. A16FM 18 100 mM Tris HCI pH 8 0 100 mM Tris HCI pH 8 0 50 500 Hl 100 mM Tris HCI pH8 0 100mM Tris HCI pH8 0 10 ml 10 ll 0 0 5 N 0 5 N 100 ll 50 mg 5 ul 5 100 mM Tris HCI pH 8 0 100 mM Tris HCI pH 8 0 495 Hl V2_ 300_JA16FM 19 V2_ 300_JA16FM 20 DD
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