iMark マイクロプレートリーダー取扱説明書 - Bio-Rad
Contents
1.2.
3.
4. Mark CE EN61010 1 EN61010 1 EN61010 1 2 000m 10 I 2
5. Life Science Research web site http discover bio rad com 1 1 BEAL 17 www bio rad com
6. PUT VIPER ICES CECT EER IMDM OP CO LO FR
7. KAENZOFEHSO VD COL Mia tO Un RARO DH ES FAX http warranty registration bio rad co jp registration htm
8. 2 2 1 1 143 0006 14 B 3 TEL 03 6404 0330 FAX 03 6404 0332 Z10632 1506G T OLE 1 1 xR E A B CIC BLOCH
9. TA 3P O 4 A INNEREN EIERN 4 BER OWN Gene ee ee ee 4 5 E BOO ariba 6 ES a o e O ee 11 PIN OG POR O OOO E CS 11 O E E 12 na a e o O II A 13 A A a en 17 ane ee 17 ODEA S sense a csc te NEN SEREIEIEENEIENSURUNEENEUNLEUEVENLUERURUIEHLERTELEHEEERUIEHEERTERDRE 17 A aAA A O E 19 AS VIDA AAA da 19 Mark Data Selector 7 7 NET aio 19 Be Oe MATAN A ee 23 Mark ii 23 TUN
10. 10 2 1 30 64 2 1 Checkmark Checkmark 1 8 2 Administrator Common User
11. 49 a STDs Standard menu TEnter p37 p56 50 STD 3
12. www bio rad com Documents TC20
13. 1 a 2 PAS 7 f 3 ww 1 4 Paper Feed 1 2 8 9 3 4 5
14. 4 Reload Range of date 10 24 2008 12 17 2010 Recent a week I Recent a month Date when read Y Date when read T Kit name Date last update Protocol E Date when read 2010 12 17 E 2010 12 17 17 12 41 Kinetic 01 2010 12 17 16 54 13 65 Date when read 2010 12 16 2010 12 16 23 05 55 Kinetic 01 2010 12 17 16 54 14 65 Date when read 2010 12 07 2010 12 07 15 36 02 Kinetic 01 2010 12115 16 20 09 65 2010 12 07 15 32 41 Kinetic 01 EE 16 20 09 65 Date when read 2010 03 18 2010 03 18 15 12 25 End point 01 2010 12 15 16 20 07 Date when read 2009 04 16 2009 04 16 21 49 33 End point 02 2010 12 15 16 20 08 2009 04 16 21 48 34 End point 02 2010 12 15 16 20 08 2000 04 16 21 39 27 End point 02 2010 12 15 16 20 06 4 Settings Mes Filter Ref Filter Shaking speed Shaking mode ls 490 3 655 4 655 4 415 1 490 3 490 2 490 2 490 2 655 4 Middle Before every read 2 Middle Before every read 3 Middle Before every read 3 Middle Before every read 3 i Start MasterPlex I Export 5 R Close 67 Mark
15. 0 9 10 EY 30 Mark N crorlate Reader 5 N 10794 Raw data report 0940542011 14 31 09 Lab name Bio Rad Laboratories kit name End puint 410 Readina mode Single Measurement Filter g 0 64 0 905 0 451 0 877 0 466 0 886 0 479 0 912 447 0 889 451 0 883 472 0 808 467 0 893 2 UM oS oe oo gt ser oS ke E Pr A 304 1 305 el 1 293 289 1 294 297 1 294 286 1 301 all 1 287 303 1 304 ae 1 917 a 3 1 319 1 308 1 292 1 320 1 284 1 302 1 294 1 312 y 1 310 1 902 1 204 1 317 1 31 1 291 1 302 1 327 ann f 6 608 1 695 1 695 611 1 677 1 683 668 1 676 1 67 BBS 1 701 1 721 673 1 671 1 688 6 9 1 687 1 608 bbb 1 686 1 697 680 1 706 1 696 1 N 12 J09 0 895 0 886
16. 4 ON 3 A 30 Administrator Common User 00000 3 System Login User Administrator IEdit Security Change Password 3 Pea Enter Security gt TBdit Security Menu Change user
17. 3 4 Main Enter 4 StartStop Memory Recall Edit Print Paper Feed kit name NZ FR M R End point 01 EndPoint Assay _ M405 1 R655 5 Shake 9998 Mid emma TTT TT M Kinetic R 01 Rinet c 1 2 aes te A ale Shake 99 s Mid Every AR ey E E Low Mid High
18. Enter Single Cutoff Control Abs Enter Ranged Cutoff Control Enter Ich CNx TCPx Cutoff Formula Cutoff Formula
19. GALT 4 bR A GALT 2 GALT R2 R1 k Kinetics 1 Absorbance ifark Microplate Reader S N 10794 N 9 Kinetic absnrbance data report 10 05 2011 Ba i Lab name Bio Rad Laboratories Kit name Kinetic 01 Reading mode Single Measurement Filter 595nm 4 Number of reads 5 Start delay time 5sec Reading Interval time sec 10 10 10 10 Shaking ON Before Ist read Time 3sec Speed Lou Calculation range 1 to 10 Reading number 10 05 2011 16 58 31 Blank Mean 0 257 Blank D 0 000 4 5 0 638 0 i 0 657 0 iJ 0 652 0 652 0 650 0 239 0 439 0 661 0 671 0 664 442 0 650 0 647 0 661 0 454 0 651 0 660 0 658 31 0 646 0 654 0 663 1 0 664 0 656 0 672
20. p52 54 p55 p56 59 Func ABC abc sym 46 Cutoff TEnter 2 Not use
21. Filter disk jamming Edit Filters Te M Filter not installed 15 16 Printer error Battery error Memory error Calendar error LCD hardware error Printer error Light level error D A conversion error A D conv
22. 1 2 1 1 2 2 1 O l 2 2 2 1 ER Z10260 110
23. 2 XTRA gt Y X Y iv FAL CC PERV 1
24. Enter 0 TEnter 12 Enter 17 Enter
25. Mode TEnter Edit GALT 2 Enter Yes No TEnter
26. Enter Tstandard Recall setting Yes Standard information setting curve fit setting STD Recall setting No Standard information setting J5 KU Tcurve fit setting End Point Protocol Kinetic Protocol Mode TEnter a b c
27. iE et 2 5 000
28. Enter Tstandard Yes STD No STD 5 STD Edit SaveSTDC 2
29. 4 X20 LCD Checkmark 3 Checkmark admintstrator Memory Recall 1 iMark BOWLS ELITE 30 3 2 00000 System Login Enter User Administrator
30. GALT k X 2 1 Enter 3 GALT GALT TA Z Edit FUNC 59
31. 100 100 c Formula 12 l k x CNx 7 k CNx A k CPx 8 k CPx gt k COx 9 k COx 4 CNx k 10 k CNx CPx De CPx k 11 CNx CPx K 6 Cox k 12 K1 CNx K2 CPx CNx 1 CPx 1 Cox l Abs 100 Abs
32. p49 11 Curve fit Concentration mapping Cd p52 54 p56 59 BAR 12 1 2 plate shaking p55 13 Main Main Start Stop 23 Mark Beer Beer 10 lo I OLED log Beer Absorbance Log lo I iMark 8
33. TEL 03 6404 0330 FAX 03 6404 0332 MAIL life_service jp bio rad com 710632 1506G Bio Rad Serial No 1 1 1
34. Main menu Edit Filters 73 1 74 2
35. Enter a Checkmark Memory Recall Plate Data Enter Select Datal type TEnter TEnter Checkmark 1
36. Enter Are you sure Yes No Enter Yes Edit a9 5 1 lt 5 Enter TA Z HF
37. 3 BLK 0 10 1 STD 0 X 1 1 Blank 1 Standard 8 Control CN 2 CW 0 CP 2 CO 0 QC 0 Calibrator 1 Sample 32 Replicate number 2 2 A B C D F H 57 58 Edit TEdit Edit Edit TProtocol TEnter
38. 2 Range of date ARO WEN Me MATE CRA RUE ROWER MOL AR CEI RET 68 ZZ gt A en N o gt AS l 1 date whenread fame O lemme fm 3 Date ist update IR a Protocol m WW 5 Mes Flter EMAIL measurement ter DIESEM Retter 0 Ref filter reference filter 7 Shaking No Before 1st read Before every read amp Kinetic DA Shaking ILow Middle THigh Kinetic FF 9 shaking time Shaking Kinetie Number of reads Kinetic ET A Settings 3
39. ba gt Change BAT Enter DotFunction N 0 EMP 5 QC QC 1 SMP 6 CAL 2 BLK 7 COP 3 STD 8 CN 4 CO 9 CW 13 14 2 iMark 16 2 Mark Mark 168 1130JA iMark 1 4 4 14 USB 1 CD 1
40. End Point Protocol Kinetic Protocol TMapping TEnter a 10 LCD 1 Blank B 2 Sample X00 X99 3 Standard S01 S99 4 In kit Control Positive Control CPO CP9 5 Weak Positive Control CWO CW9 6 Negative Control CNO CN9 7 Cutoff Control CO0 CO9 8 Calibrator CLO CL9 9 QC Control QCO QC9 10 Empty well 0 EMP 1 SMP 2 BLK 3 STD A CO Cutoff 5 QC QC 6 CAL
41. Enter Edit TA Z MEO Edit FUNC STDC Edit Save STD Enter STDC 1 lt 5
42. K Enter sn L Erchk 2 k CPx 3 k COx 4 CNz k 5 CPx k 6 COx rk 7 Xx CMx amp k CPx 0 k COx me 10 k CNx CPx Mei 11 CNk CPx k MT Sam 12 k1 CMNx k2 CPx ae me E Cr E CF rem Coefficient for Cutoff Formula 1 to 11 ix GPa E 1 11 rk Cutoff Formula Enter Coefficient for Cutoff Formula 1 eee es 12 imput comEFicient fk1 Tk2 ELEF Cutoff Formula Ente
43. 3 3 iMark AC 100 120V 1 2 A 10 6 50 60 Hz ax ea AT 15 40 C 90 2 000m I 10 50C 90 EN 61010 1 2 Mark ELISA Mark CE EN61326 A iMark
44. FR as USB2 0 3 Low Mid High 0 999 10 30 2 DART AY TNE 64 2 3 1 2 8 1 20W 3000 400 750nm 3 0nm 10nm 8 Fast 6 1 10 2 Step 15 1 25 2 0 000 3 5 OD 0 001 OD 1 0 0 010 0 3 0 OD 490nm
45. TEnter Fast Step Normal Evaluation ES Fast Step Normal Eval Enter Fast reading 6 1 10 2 Step reading 15 1 30 2 1 U V Normal mode 1 Evaluation mode 4 53
46. Before Every First Speed Mid Hi Low Time Enter Photomeric Single 1 Dual 2 Enter
47. TEnter Edit TProtocol TEnter CC Limit TEnter GALT Enter p38 40 Enter
48. TeSeE Jabs Ss Endpoint Protocol Position 5 TeSeE eq Bndpoint Protocol Position 6 2 007 TeSeE RT ES 1 lt 0 150 3 2 1 400 2 3 gt 1 000
49. 4 Cutoff Constant Cutoff Control Cutoff Formula Cutoff Ratio NR E a Cutoff Constant Cutoff Constant Constant Single Ranged Cutoff Constant Ranged Cutoff Single Cutoff Enter Single Cutoff Constant LES Unit Single Cutoff Enter Constant Ranged Cutoff Constant
50. Abs A ELA Aik T Abs 100 Abs Abs 100 Abs Abs a 4
51. Administrator Edit Menu Edit TEdit Edit Protocol TEdit Seria Rep type Simple Full S1 S4 405nm gt 450nm gt 492nm gt 5
52. TAN MT qez T J Eu Light bulb error u A AP BUCK O ld ha a o
53. liMark Data Selector 15 15 66 iMark PC Mark Mark Mark Data Selector Mark USB iMark Data Selector IiMark Data Selector iMark Data Selector N alas All Programs iMark Data Selector iMark Data Selector iMark iMark Data Selector iMark 2
54. MR NL m t Plate carrier error es a 16mm 16mm O Edit a E PR 1SS1 8 id Xam Hl fC Filter missing 9
55. Install C Program FilesYHitachiSoftYMasterPlex 2010 rn A en a y E E w E ry oe fer I dark Gata Seler Tor Ir stal Bs hield Wizard Ready to Install the Program The wizard is ready to begin installation Click Install to begin the installation TF you want to review or change any of your installation settings click Back Click Cancel to exit the wizard InstallShield 4 Finish iMark Data Selector MasterPlex ReaderFit MasterPlex ReaderFit CF iMark Data Selector iMark MasterPlex ReaderFit MasterPlex ReaderFit
56. 4 Close Settings Settings Settings Mark Folder OK settings Storage Folder 1 ProgrambsatalHibachi Software MasterPlex BioRad ModuleRa Folder OK Cancel Windows XP C FDocuments and Settings All Users Application Data YHitachi Software MasterPlex BioRad Module Raw Data Windows 7 C ProgramData Hitachi Software MasterPlex BioRad Module Raw Data 69 70 12 7 IIS AIR NI NN
57. Enter Plate auto mapping completed 55 96 Yes TNo B S X CL C S X CL C 1 1 Blank 2 Standard 1 gt 12 3 Control CN 19 4 Control CW 19 5 Contro
58. nn a b C d Paper Feed e Mark 4 Main Edit 6 Language Bngliish Enter 2 3 Enter
59. TeSeE 0 210 0 210 0 900 TeSeE eq 0 210 0 090 POS gt NEG lt 202 E 2 34 Edit Ranged Cutoff b i Single Cutoff Ranged Cutoff Single Cutoff 12 i i neh
60. O HN DD a A 0 N m m ESC P ESC P 4P Logistic Rodbard 4p logsiticRodb 5P Logistic Rodbard 5p logisticRodb AP Logistic Cook Wilkenson 4p logisticCook 5P Logistic Cook Wilkenson 5p logisticCook 5P Exponential Sigmoid Logistic Quadratic regression Linear regression Cubic Point To Point regression 7 Concentration Abs Ratio Mark Nicrorlate R
61. ICP 8 CN 9 CW 2 F Nm S01 S01m 54
62. 6 Mark xMark USB Mark USB 2 0 Start Stop Open Close Remote mode Start Stop Mark
63. 168 9520JA 6 Windows Mac OS X 168 1130DS iMark Data Selector 168 1006 iMark 680 Foodmark 168 2007 iMark 3 168 1010 iMark 680 400nm 400 168 1011 iMark 680 405nm 405 168 1012 iMark 680 410nm 410 168 1013 iMark 680 415nm 415 168 1014 iMark 680 420nm 420 168 1015 iMark 680 425nm 425 168 1016 iMark 680 430nm 430 168 1017 iMark 680 435nm 435 168 1018 iMark 680 440nm 440 168 1020 iMark 680 450nm 450 168 1023 iMark 680 465nm 465 168 1024 iMark 680
64. F Blank CN CW CP E CO QC QC Calibrator Sample Replicate well Func ABC abc sym Administrator Common User HE Administrator 128 000 _ Funcj t ABC abc sym Save STD Curve 5 Rename STD Curve 5 Funcj ABC abe sym Select Language e 30
65. English jJPN Mark Power off Yes gt gt Yas Bower off iMark Data Selector iMark Data Selector iMark USB Windows PC CSV 6 19 20 3 3 Mark 22 3 iMark iMark Mark os
66. 45 Edit Menu Edit TEdit Edit TProtocol TEnter p31 33 Enter OD OD 0 000 3 500 Enter
67. Enter Security 17 18 Edit EDIT Filters Enter Enter Main Enter Back EDIT Filters EProtocol Lab name eSecurity Save STDC Filters Language Date set LCD adj Edit EDIT Date set Enter Main EDIT Date set Day Month Year D E Hour Minutes E E Protocol Lab name eSecurity Save STDC Filters Language gt Date set LCD adj
68. LA iMark Data Selector CD 1 1 1 iMark 168 1135JA 6 168 9520JA Mark PC 6 168 9520JA PC 1 Mark 2 3
69. 1 Single 2 Dual Measurement 2 Reference 2 Dual Single Dual Edit Filters TEnter 0 999 YES NO Low Mid High
70. 3 JPN Enter 4 Yes Enter 5 eProtocol Lab name Select Language Power off eSecurity Save STDC English EJPN Filters MLanguage Russian MYes No Date set LCD adj Chinese 61 62 6 6 iMark Data Selector 64 6 Mark Data Selector DOS V Windows XP 7 OS NET Framework 3 5 SP1 Pentium 4 1GB 120MB 2 i 1024x768 65536 16bit DPI 100 CD ROM 1 CD
71. Microsoft Word Microsoft Excel Microsoft Power Point Microsoft Corporation Microsoft NET Framework Microsoft Corporation Ex MasterPlex Windows MicrosoftY Windows Operating System Word Microsoft Worde Gd Bxcel Microsoft Excel Cv PowerPoint O EINA ERTA Microsoft PowerPoint CT MasterPlex MasterPlex iMark Mark Y Copyright Copyright 2010 Bio Rad Laboratories Inc
72. TEnter I mel 11 gimml 2 m mall 13 U ml 3 umoll 15 Ul 4 nmoll 14 om 5 pmol 15 mIU ml 6 mel l EU 7 mel 17 Arbit pel 2 und 10 ng ml b 2 Enter 10 Fi TE Enter Enter 1 AP Logistic Rodbard 4p logsiticRodb 5P Logistic Rodbard 5p logisticRodb AP Logistic Cook Wilkenson 4p logisticCook 5P Logistic Cook Wilkenson 5p logisticCook 5P Exponential Sigmoid Logistic Quadratic regression Linear regres
73. NET Framework 3 5 SP1 MasterPlex ReaderFit iMark Data Selector Install InstallShield Wizard i Mark Data Selector requires that the following requirements be installed on pour computer prior to installing this application Click Install to begin installing these requirements Status Requirement Pending MasterPles ReaderFit 20 Ae Install Cancel 2 NET Framework 3 5 SP1 MasterPlex ReaderFit iMark Data Selector Next gt 65 Welcome tn the InstallShield Wizard for irlark Data Selector The InskallShield R Wizard will install Mark Data Selector on your computer To continue click Next WARNING This program is protected by copyright law and international treaties 3
74. 41 Edit TEdit Edit Edit Security Enter Change Password Current TNew TEnter Change user Change Enter Lock Unlock Chang
75. gt Concentration 0 9999 Unit f E 17 Ranged S Cutoff val gt Cutoff AX Es Single Cutoff _ Raw Abs Lim MtX Cut CrV Cnc Dif E Upper Limit Lower Limit Number of STD 0 2 12 l 0O 9999 17 10 4 Log Lear Recall Setting Select STD Cum 5 Set Photo mode 1 2 Set Shaking F Yes No Low Mid High set Read mode A Fast Step Normal Eval Func N F N
76. TEnter 17 Ranged Cutoffj Single Cutoffj Enter Single Cutoff Control TEnter EF Ranged Cutoff Control Enter
77. 100 09 10 T d Ratio 32 6 Curve Fit Mark 10
78. 470nm 470 168 1025 iMark 680 475nm 475 168 1026 iMark 680 480nm 480 168 1027 iMark 680 485nm 485 168 1028 iMark 680 490nm 490 168 1029 iMark 680 495nm 495 168 1030 iMark 680 500nm 500 168 1031 iMark 680 505nm 505 168 1032 iMark 680 510nm 510 168 1033 iMark 680 515nm 515 168 1034 iMark 680 520nm 520 168 1035 iMark 680 525nm 525 168 1036 iMark 680 530nm 530 168 1037 iMark 680 535nm 535 168 1038 iMark 680 540nm 540 168 1039 iMark 680 545nm 545 168 1040 iMark 680
79. L Ranged Cutoff TEnter Constant Ich Constant Absorbance Concentration Abs Constant Absorbance Constant JEF 47 b C 48 Cutoff Control Cutoff Control CPx CNx Single Ranged Cutoff Control Ranged Cutoffj Single Cutoff
80. EF lo I Lo Mark Administrator Common User 2
81. e Main 37 5 5 40 5 Initial Startup Self diagnosis Initialization System Login Common user Administrator 00000 Edit Security TStartStop
82. 5 24 Administrator Common User 00000 Bio Rad Laboratories nm 1 1 00 0 0 A Mark OD 3 500 3 5 Tr 4 500 4 500 25 26 4 4
83. Edit FUNC 43 Language Select Language gt English Russian Chinese TEnter Power off Yes No Yes Enter Enter 44 Print Repor
84. 10 Concentration Curve Fit BSE ranged constant Single constant formula ratio 4 x iMark Microplate Reader S N 10794 1 Raw data Absorbance report 1 REM lah a aal iy CORREN 2 Kit name End point 10 Reading mode Single Measurement Filter 415nm 1 Blank Mean 0 464 Blank S D 0 000 2 3 0 000 0 441 0 855 0 007 0 413 0 844 002 0 422 015 0 448 017 0 425 013 lt 1 224 1 231 1 231 1 207 1 213 1 219 828 1 204 1 212 1 207 856 1 219 1 237 1 257 1 209 1 207 1 224 1 215 1 223 1 224 1 191 1 232 1 228 1 216 1 242 1 232 830 830 B48 0 0 0 429 0 838 0 0 0 0 0 445 0 429 9 10 B41 0 846 0 845 0 431 0 432 829 0 838 0 836 0 432 0
85. TEL TEL FAX Km REES S N
86. Absorbance Kinetic plot Linear regression General Kinetic Assay GALT report GALT Kinetic assay pe Func ABC abc sym Administrator 1 8 000 _ Func ABC abc sym Save STO Curve 5 id 5 Func ABC abe sym SaveS TDC r 1 64 1 2 Checkmark 1 F Report Print Protocol Pe
87. 1 0 0 2 0 OD 2 0 0 3 0 OD 1 0 0 005 OD 02 0 OD 1 5 2 0 3 0 OD 1 5 0 005 OD 0 3 0 OD 80mm 100 240VAC 50 60Hz 100VA 34 6 W X 37 7 D X 16 4 H cm 9 5 Kg 15 40 C 90 10 50 90 7 7 Ordering Information 168 1130JA Mark 168 1135JA iMark Microplate Manager 6 168 1130J1 iMark PC Microplate Manager 6 168 1130J2 iMark ELISA Immunowash1575 168 1130J3 iMark ELISA Immunowash1575 Microplate Manager 6 168 1130J4 iMark BLISA PC Immunowash1575 Microplate Manager 6
88. Appendix 1 2 eee re RE Mark
89. iMark 28 4 iMark 2 9 TeSeE 9 Endpoint Ta 0 9 10 Cutoff Limit Endpoint Cut Off Cutoff
90. 11 12 Da This product conforms to the class A standards for Electromagnetic Emissions intended for laboratory equipment applications It is emissions from this product may interfere with some sensitive appl kite ole at po an ake appropriate measures OM ma an USB 2 0 Main Start Stop Paper Feed Print Edit Edit Memory Real Open Close de T FRA
91. 300 0 896 0 873 30 0 862 0 883 des 0 807 0 901 306 00 899 0 882 310 0 899 0 8 1 309 0 860 U UBE 429 0 903 0 898 u abe m a E E E E ee iMark Micrnplate Reader S N 10794 Limit report 09 05 2011 14 1 7 09 Lab name Bio Rad Laboratories Kit name End point 10 Reading mode Single Measurement Filter 415nm 1 000 abs 500 abs Upper Limit 1 Lower Limit 0 ET nm 95 gt x A E E E E E 0 0 5 10 11 12 x Y 2 Y ur Y eC Y 3 3 Oo iMark Nicrorlate Reader S N 1 Natrix report 09052011 14 17 09 Lab name Bio Rad Laboratories Kit nane End point 10 Reading node Single Nessurement Filter 414ne 1 eper Limit 1 000 abs ower Limit 0 500 abs U L commas N mMm 3 TO 4 4 F t 5 05 i nn oo oo Oa i j 00 05 05 om 0 mo 05 0 05 oD 5 Me 5 Cutoff iMark Microplate Reader S N 10704 Cutoff report 090542011 14 17 08 ranged constant single constant formula ratio 4 pri er e a Reading node sin
92. 6 0 655 0 6 6 653 LU oo o 63 61 52 Do nM 0 gt J y c oo cC 0 4 4 9 10 11 12 0 448 0 452 0 454 0 447 0 236 0 225 0 443 0 449 0 457 0 453 0 233 0 218 gt 0 453 0 454 0 454 0 456 0 233 0 221 460 0 445 0 460 0 468 0 260 0 223 56 0 448 0 456 0 437 0 228 0 219 65 0 464 0 466 0 453 0 214 0 217 53 0 455 0 458 0 458 0 222 0 207 62 0 463 0 464 0 462 0 238 0 219 0 0 4 0 4 we 0 4 2 Kinetic Plot iMark Microplate Reader S N 10794 2 A aN Kinetic Plots report ESC P ESC P 10 05 2011 16 58 31 Lab name Bio Rad Laboratories Kit name Kinetic 01 3 Linear Regression Reading mode Single Measurenent Filter 6964 umber of reads 5 Start delay time sec US Reading Interval time sec 10 10 10 10 4 GALT ae A E EE NEE Li Sue Shaking ON Before Ist read GALT 2 GU Time 3sec Speed Low Calcu
93. iMark 168 1130JA 168 1130J1 168 1130J2 168 1130J3 168 1130J4 168 1130J5 168 1135JA AM9 00 PM5 00 JST 03 6404 0331 Fax 03 6404 0334 Email life ps_jp bio rad com Web Web www bio rad com Trademarks and Legal Notices Adobe Acrobat Adobe Systems Incorporated Microsoft Windows Windows XP Windows 7 Windwos 8 Microsoft Corporation
94. 0 9 b 9 BLK Blank 0 lt 10 CN ControlCN 0 lt 9 CW Control CW 0 lt 9 CP ControlCP 0 lt 9 Control CO 0 lt 9 QC ControlQC 0 lt 9 CAL Calibrator 0 lt 9 SMP Sample 0 lt 96 REP Replicate well 1 4 un AL NR NN O B X CL C S Standards Setting Replicate well
95. Password iii Press ENTER 3 p60 61 p48 59 4 p47 5 p48 6 Cutoff Report Limit STDs Mode Mapping kit name parameters 7 a 20 a 8 plate mapping p56 9 Matrix Limit p48 10 Cutoff
96. 1 Select Mapping Mode Yes 2 Enter STD 1 B 2 X S 00 99 3 S 01 99 4 CN CP CW CO CL
97. 550nm 550 168 1041 iMark 680 555nm 555 168 1042 iMark 680 560nm 560 168 1043 iMark 680 565nm 565 168 1044 iMark 680 570nm 570 168 1045 iMark 680 575nm 575 168 1046 iMark 680 580nm 580 168 1047 iMark 680 585nm 585 168 1048 iMark 680 590nm 590 168 1049 iMark 680 595nm 595 18 168 1050 168 1051 168 1052 168 1053 168 1054 168 1055 168 1056 168 1057 168 1058 168 1059 168 1060 168 1061 168 1062 168 1063 168 1064 168 1065 168 1066 168 1067 168 1068 168 1069 168 1070 168 1072 168 1074 168 1076 168 1077 168 1078 168 1079 168 1080 1 2 3 iMark 680 600nm iMark 680 605nm iMark 68
98. 700nm Mark 680 710nm Mark 680 720nm Mark 680 730nm Mark 680 735nm Mark 680 740nm Mark 680 745nm iMark 680 750nm 1 1 1 1 1 1 1 1 1 1 1 1 1 1 iMark 680 400 750nm 600 605 610 615 620 625 630 635 640 645 650 655 660 665 670 675 680 685 690 695 700 710 720 730 135 740 745 750 400 750 2 400nm 750nm 2 19 Appendix 80
99. 0 610nm iMark 680 615nm iMark 680 620nm iMark 680 625nm iMark 680 630nm iMark 680 635nm iMark 680 640nm iMark 680 645nm iMark 680 650nm iMark 680 655nm Mark 680 660nm Mark 680 665nm Mark 680 670nm Mark 680 675nm Mark 680 680nm Mark 680 685nm iMark 680 690nm Mark 680 695nm Mark 680
100. 1B Visit us at http www bio rad com 140 0002 T 532 0025 812 0013 2 2 24 1 14 11 2 5 28 MNL1681130 Mail life ps_jp bio rad com Tel 06 6308 6568 Tel Tel Tel 03 6361 7000 092 475 4856 03 6404 0331 Fax Fax Fax Fax 03 9463 8480 0663083064 092 474 5580 03 6404 0334 M10113 1506D
101. 409 830 0 830 0 837 0 398 0 429 830 0 853 0 862 0 443 0 437 837 0 847 0 842 0 435 0 418 823 0 827 0 846 0 425 0 413 840 0 838 0 845 0 416 0 422 0 857 0 853 0 863 0 865 0 439 0 434 29 2 Absorbance Abs Raw Blank mean Blank mean X n S D X 2 n Blank mean 2 n 1 1 2 S D X X 2 2 n 3 Limit 77 4 Matrix
102. 40nm gt 620nm gt 690nm Enter Max CV S1 S4 Max CV Enter 405nm 492nm 540nm 690nm Enter 60 Mark 1 Edit 2 Language Enter
103. E TA SIDA diodes 23 ee ee ee ere 23 A aay asada E 24 een eek 24 RE A E 24 der AIMAR Oe NA 29 sd ba e IT A A 29 1 Raw data ki 29 Dx IN ICE Absorbaneo A 30 UN Ei A 30 Me NATA MAA 30 Nee O IO 31 EZ IL EWR A NA 33 T RE Concentration LAR Bean crate incnodetnveutetveveedevereudeks 33 0 a SS een 34 9 Re RC GR ed 34 TASA ee e cee ein ee eed 35 RR ri 36 te WE Absorbance NAS a hh 36 De Kinetic Pl Tea 36 3 NE Sr elle Linear Resresson RR sestiaeevevuaneenveeadeseeends 36 A A IN IE EIER SSR 36 37 miete gr ae a a erweisen 41 TS oia 42 J RS AI nennen 45 AA 46 De see 50 A elle o 53 DR 0 ee EE ee ee eee ee eee 54 i 58 VERS A es ee 59 Re PODIO TE aan 61 6 iMark Data Selector es 65 coco 65 A 65 iMark ORE 3 A O o Ie 67 iMark Data Selector SARs RR 67 AA iia 67 O te eho yaa 772 ee 73 EN ET ne ee ci 73 occ ccccccccnsecccce
104. ceecceuececaceeensueeenaneeseenaneesaaneess 73 LE RTS Nii 74 ee 75 iMark IA a SS a ices a R 77 Ordering Information iii ia 8 Appendix es 80 10 1 Mark 168 1130JA 168 1135JA 168 1130J1 168 1130 2 168 1130J3 168 1130J4 8 96 1 2 3 Mark 9 Mark USB 2 0 6 168 9520JA
105. e Lock Unlock TEnter Lock Common user TUnlock Checkmark Administrator Lock Unlock ERS 000 Enter Edit
106. eader S N Curve Fit graph report 091052011 14 17 09 Lab name Bio Rad Laboratories Ail name End Point il Reading mode Single Measurement Filter 415001 Blank Mean 0 464 Blank 5 D 0 000 Curve fit Eure Linear resression Concentration unit mol l Conc 5 000e 01 000e 00 500e 00 2 000er00 Y A Hex Slore 5 502000e 01 Intercept 2 407500e 01 Correlation coefficient 9 421957e 01 Standard error 093783e 01 Grarh axis X Linear Y Linear an3 1 5 L LT VE it 3E u Point Lo Point regression centration urt nols STOE Conc iba ella 3101 5 000e 01 0 427 42 82 STi 1 U00et00 0 050 AR BS 5103 1 500e 00 lb A Be 2 000e 00 Die 17 nun sample 5 0248 01 0 4239 F2 E F 5 9 te ET ite at 33 8 Difference A B C D Fil E F ierk Wiroplate Reader S N 10794 ifference report Row G H 09 05 2011 14 17 09 Lab name Bio Rad Laboratories Kit name End point 10 Reading mode Single Measurement Filter 415nm 1 Subtraction Odd Even 9 TeSeE lt BSE gt TeSeE 2
107. ersion error EEPROM error USB error 1 Sorte ree ous 400 750nm iMark AA SR
108. gle Measurement Filter 415mm 15 a Ranged Constant Cutoff ae Constant value Ranged cutoff Positive value 1 000 ab Negative value 0 40 Abs Bi b Ek E LK Y A E E gt E Abs ARA Neem HI Tt t E E Y YA LS Ek g EM x E E Y Y E E j E Y E E Y Y E E E ET Y Y ES b Single Constant Abs
109. l CP 19 6 Control CO 1 9 7 Control QC 1 9 8 Calibrator 1 9 9 Sample 1 96 10 Empty well Cutoff Control TCuoff Formula Cutoff Ratio S A1 H1 A2 gt H2 A12 gt H12 smig Ss 96 Blank Standards CNs CWs CPs COs QCs Calibrators Sample Replidates 96 SAS 1
110. lation range 1 to 10 A GALT R2 R1 k R1 1 Abs R2 2 Abs k GALT 36 Edit 2 30 delay 0 lt 999 First Every disable Low Mid High 1 2 Fast Step CC Limit GALT factor k 0 9999 Result 2 read 1 read xk Calc range GALT assay Func N F N F Blank CN CW CP CO QC QC Calibrator Sample Replicate well General GALT
111. r Ich CNx TCPx d Cutoff Ratio Cutoff Ratio CL 3 TEnter
112. sion Cubic Point To Point regression ze NO NM e Graph exis 4 TEnter Enter 1 Log Log X Log Y Log 2 Log Linear X Log Y Lin 3 Linear Log X Lin Y Log 4 Linear Linear X Lin Y Lin ol b STD Recall STD Curve Setting SaveSTDC 92 Select STO curve Right arrow key T STD STD 2 1 Recall Yes No 2 1 lt 5
113. t Enter Protocol Enter Start Stop TMemory Recall TProtocol Enter Select protopcol type TEnter
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