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Contents

1. pH 3 3 1 1 2 30 3 3 2 30 50 50 100KPa
2. i N a A EN 1 Ne N i i i
3. i i i i TE WE ARR RE 1 2 et 1 3 1 3 1 3 2 4 2 5 2 5 1 5 2 pH 5 3 5 4 5 5 2004 8 1 TOYOPEARL AF Chelate 650M TOYOPEARL HW 65 MCAC Metal chelate affnity chromatography 2 MCAC Porath
4. 4 ck 3 i i i i i i
5. Ni Co 5 2 pH pH7 9 EDTA 0 5 1 0mol L NaCl 5 3 pH pH a pH pH pH7 pH3 pH s d EDTA EGTA
6. 5 4 50mmol L EDTA 5 5 0 5mol L NaOH 0 5mol L HC1 6 20 4 35 HLC TSK GEL TSKgel TSKgel SuperMultipore BioAssist Enantio PStQuick Enviropak TOYOPEARL ToyoScreen TOYOPEARL GigaCap TOYOPEARL MegaCap TOYOPEARLPAK TOYOPAK TOSOH 03 5427 5180 G 06 6209 1948 G 052 211 5730 0120 17 1200 105 8623 541 0043 460 0003 252 1123 3 8 2 4 4 9 1 17 13 2743 1 Printed in Japan
7. HPLC d 4 TOYOPEARL AF Chelate 650M 1 e 40 mol e d 2 d 3 pH 0 5 1 0mol L NaCl 4 0 05mol L EDTA 5 5 1 Cu Zn Cu Cu Zn Cuz
8. TOYOPEARI TOYOPEARL AF Chelate 650M gt TOSOH i i i i i i

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