Flexchip 日本語取扱説明書
Contents
1. OFF TEL 03 5331 9336 AL 9 00 17 30 www gelifesciences co Jp e mail2. UN ka 1 Ms Ka 1 s g Kp kq k Rmax RU Conc M i 0 25 H Calculations Report Point Table Report Point Plat 8 B G u a Fla sp 8 8 E rr rrr Residuals Residuals plot Flexchip Handbook 8 63 Save AS Analysis name for all groups Help Cancel Save l Close Flexchip Evaluation Software Completed Analysis 2 Flexel p Evaluation Software C AFLEXCHIP 24ata
3. Idle Method Hun ldlg Method Method 1C SFLEXCHIP Methods WwaterFlush1 idle m N After system has been idle for 240 minutes Miscellaneous Syringe Maintenance Needles Maintenance System Purge Buffer Pump Calibrate Flexchip Handbook 10 81 2 gt Pow ET Eo s Chip Drawer Control Progress u Z Chip in place Saye j m 3 Calibration Type Selected Cancel 3 Buffer Line Primed 3 Calibration Point 1 Measured EE RM 3 Calibration Point 2 Measured pEHREERERERERERRERRNRERHNRR Select Calibration Type m Calibrate New Tubing Recalibrate Old Tubing Ready for calibration Prime Buffer Line Valve Position 1 to Chip Calibration Point 1 Calibration Point 2 T Stat Pump m 2 um Buffer Pump Factor rpm ul min 0 12 Pump Offset RPH 0 Flexchip Chip Drawer Control Select Calibration Type IRecalibrate Old Tubingl Caliblate New Tubing
4. l Flexchip Control Software Flexchip Handbook 74 10 10 Flexchip Flexchip Control Software Configuration Instrument Maintenance SE Flexchip Control Software Flexchip System Chip Definition Method Editor NENNEN 13 10 1 10 Method Setup Wash after run 76 lt gt 70 Milli Q Flexchip Handbook 10 V gt lt gt Configuration Instrument Maintenance
5. IOKI Buffer 6 l cx r EER Please accurately measure in microliters the amount of fluid collected in the tube at the calibration port and enter the amount on the form OK Milli Q ul 1000 19 Enter Measured Volume in microliters Flexchip Handbook 84 10 BE SEITE 5E Chip Drawer Control Progress 1 0 Qut Chip in place Z Calibration Type Selected Z Buffer Line Primed Z Calibration Point 1 Measured 3 Calibration Point 2 Measured 3 Factor Calculated Cancel Ready for calibration Select Calibration Sissit ning T Prime Buffer Line Valve Position 1 to Chip Prime Line Now Calibration Point 1 Use Recommended Settings Enter Measured Volume in microliters 0 14 HUEEEEEEEEEENBEEEENEEEEEEEENEEEEENHENNEN m Calibration Point 2 Use Recommended Settings Start Pump 3 1 u Buffer Pump Factor rpm ul min 0 12 Pump Offset RPM Calibration Point 2 Use Recommended Settings
6. Run Flexchip Handbook 7 49 gt Fill Options Select Fill Option Bubble Blast Select Bubble Blast Buffer Running Buffer C Execute 1 NN Method Run 2XMethodsXTraining Kit spr F M 9 v Method Setup W Buffer Setup Sample Setup XX Chip Setup X Run Prepare Fluidics Status Performing Blast 1 minute remaining Fluidics Prep is complete Intensity 83 482 64 Load and Prepare Chip X Chip is ready Approve R lMatri ROI Matrix Settings R lMatri is Ready NN Fill Options Select Fill Operation Bubble Blast Y Select Bubble Blast Buffer Running Buffer Y Chip has been filled m m BL A Source Angle Image Llpdate E m nina m u Apply BEN Method Run C FLEXCHIP Methods T raining Kit oooxx spr oT v Method Setup Buffer Setup Sample Setup Chip Setup BRL K K K K K K K K K K K K EE BEN I K K K K K K K K K K K H Result File ROI Information Method Progress Selected Options Events m Result Fi
7. Approve ROIMatrix Matrix Settings ROIMatrix Settings Settings for Fill RDIMatrix Columns 8 Rows Create Reference HHls Reference Size Factor Reference ROI Pattern Checkerboard v 328 78 ROI Finding Approx ROI Diameter pixels 15 Spot ppearance Dark Spot on Light Background ROI Columns Rows PReference ROI Size Factor 0 9 ROI 90 Reference ROI Pattern Checkerboard ROI ROI ROI Finding Approx ROI Diameter pixels 15 Spot Appearance Dark Spot on Light Background OK Flexchip Handbook 44 7 ROI Clear All ROI
8. W Text file import settings C Documents and SettingsMdministratnrMDesktnp 24samples x 1 2z 3 4 5 5 B Import file eo o e 828060 eo o OE E EE eo o 6 9 9 o6 E Documents and Settings Administrator Desktop 24samples 4spots tt Browse eoo o 028056068 PESERBERERERREERERRERRERERERREREREREERERREREREREREREN e a eo o 69 e00 5 p o B Spotter configuration B 2 H 6 il e m Number of rows on chip 12 2 C Fill by row Select spotting direction on chip i E m Number of columns on chip B gt Fill by column r Change chip orientation E i i E Number of replicates for each sample gt Pr E EM x X _ m 4 Change start position k A m Number of required samplesinfle 24 _ Number of required samples in file 24 E mi a suu p M Fle chip configuration Columns EE Matrix format and chip orientation in Fleschip Rows 2 Eg m Import options File requirements Read samples in listed order Sort samples by wellid Source plate size 96 m m m wel Id Content Conc Sort direction E A1 Sample 10 mM C Sample B 20 mM E12 Sample C mM H Required Required Optional 2 A3 Help Next gt Cancel Next
9. a D Create group Flexchip Evaluation Software C WLEXCHIP Fie Chip Layout Completed Analyses Resu File Properties Taraqu Sensorgram Reference Corrected Ey Truy m L Response m z i spot contenta meet spet contents al amp amp dd dd dd amp amp dd k h A od od ode ded od d de od 4 d d d x44 EE E E E E EEEE amp ood amp amp amp amp amp J i m mal roomiock vY mdustment column reference connected daba ww 2 ROI map Create groups based on spot contents Create Groups Create analysis groups based on matching entries for Content amp concentration 4 groups C Content 2 groups Help Cancel Content amp Concentration Content DKI Flexchip Handbook 58 8 HOI Referencej Aus
10. Prep Chip Required Do not run Fluidics Prep with a new chip intended for If a used chip is loaded click OK If an analysis chip is loaded replace it with a used chip and then click OK Chip Drawer Control Out Cancel Begin Prep Prep Chip Required 7 OK NN Method Run C FLEXCHIP 2wethodsxTraining Kit spr op oH o amp j t v Method Setup Buffer Setup Sample Setup X Chip Setup Run B K K K K K K K K K KE U Prepare Fluidics Status Bypass Prep Preparing Fluidics 10 minutes remaining m i W se Fluidics Prep has not been run Intensiy 766 6 2436 E Load and Prepare Chip GE Bypass Jog X Chip has not been loaded Approve R lMatrix ROI Matrix Settings R lMatrix has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute Chip has not been filled Source Angle Image Update r 13 007
11. Buffer Sample Buffer 3 Flexchip Control Software The duration of the previous step must be at least 3 minutes long in order For the sample of the current step to load Read Only Locked by Dwner Analysis Temperature 25 0 Dorp V ChipFlush1 x s Eo Blocking 3 50 Perform wash Before Run ChipFlush2 Cycles Pause min EEEHEHNEHEHEHHEHHEHHHEHRHEHEHRHEHRHREHEHRHEEEENE N a W S SUS m Flexchip Handbook 28 5 Step2 Operation Set1 Sample1 Read Only Locked by Dwner Analysis Temperature 25 0 Wash Prime Load Options Chip Flush 1 Cycles Pause min Prime Buffers Before Run Wash Sample Paths After Injection Blocking Buffer Parlorm Wash Before Run Perform Wash After Run Pause min ChipFlush2 Cycles m 1 U K K U K K K K K K K K KK KL KS K K K K KU K pH Pm l L m kus UNO Recirc
12. 2 Flexchip Handbook 11 91 11 System Check Method Flexchip 110 gt Flexchip Test Solution Kit BR 1007 24 Test Solution 1 Test Solution 2 Gold Affinity Chip BR 1007 00 Sample Tubes 5ml BR 1007 06 Milli Q 1000ml lt gt Milli Q 1 VP1 6 VP6 System Purge l 5 L Z Flexchip Test Solution 2 2 Mili Q 1 6ml Flexchip Flexchip Test Solution 1 Flexchip Test Solution 2 Water Flexchip System Method Run Open Method 7 C FLEXCIP 2 Methods System Check spr Flexchip Handbook 92 11 MW Method Run 2WethodsYSystem Check spr F X v Method Setup Buffer Set
13. lt gt Top Spring Plunger Bottom Section Bottom Front Spring Plunger Top Section Bottom Section Bottom Side Spring Plunger Bottom Top section section Ba Spring plungers Flexchip Handbook 6 31 Bottom Section Front Side Spring Plunger 1 4 1 4 turn Bottom Section Flexchip Handbook 32 6 Bottom Side Spring Plunger 1 4 Bottom Front Spring Plunger 1 4 Bottom Section 1 4 turn Pre Gasket Window Top Section Top Section Spring Plunger
14. Read Only Locked by Dwner Analysis Temperature C 250 K K IK KK K K K K K K KK K K K K K K K K KU j Pre run Options P W Chip Flush 1 Cycles Pause min Blocking Buffer 3 50 Prime Bulfers Before Run Wash Sample Paths After Injection Chp Fiush 2 Wi Iv Pelom Wash After Hurt Cycles Pause min M e 159 Total program duration 0 00 minutes Wash Prime Load Options LIPerform Wash After Run VP6 Flexchip Handbook 26 5 Read Only Locked by Dwner Analysis Temperature 25 0 Pre un Options Mash Prime 7 Load Options Chip Flush 1 Cycles Pause min Blocking h gt 3 50 Prime Buffers Before Run Wash Sample Paths After Injection Perform wash Before Run Perform Wash After Run ITITTTITTTT ChipFlush2 Cycles Pause min Total program duration 0 00 minutes BENBENEHEHEHHEHEHHEHEHEEHEHEHEHEHEHEHEHHEHEHHEHEEHEHHEHEHEHEHEHEHEHEEHEHEHEEHEHEEHNH Step1 Operation Buffer Buffer
15. 23 1 31 5ml 10x Blocking Buffer 3 5ml 50ml 2 gt Gold Flexchip Blocking Buffer 10x BSA Streptavidin Biotin Flexchip NeutraAvidin Blocking Buffer 10x BSA Protein Flexchip Handbook Ji 3 3 1 6 6 Way lt gt Buffer pump 6 way valve Syringe pump Sample pump Buffer degasser Buffer bottles Flexchip Handbook Clamp Clamp 1 6 6 way 5 Milli Q
16. W Chip Definition Text import C Documents and SettingsM dministratoriDesktopX24sample BR Chip orientation ii BE Spot types Number of columns fB m Signal Background Show concentrations sample04 sample07 samplei0 sample13 samplei samplei9 sample22 sample04 sample07 samplei0 sample13 samplei samplei9 sample22 sample04 sample07 samplei samplei3 samplei samplei9 22 sample04 sample07 samplei0 sample13 samplei samplei9 sample22 sample05 sample08 sampleii samplei4 samplei 7 sample20 sample23 sample 5 sample08 sampleii samplei4 samplei 7 sample20 sample23 samplen5 sample 8 sampleii samplei4 sample17 sample20 sample23 sample 5 sample08 sampleii samplei4 samplei 7 sample20 sample23 sample06 sample09 samplei2 samplei5 samplei8 sample21 24 sampleO6 sample09 samplei2 samplei5 samplei8 sample21 sample24 sample06 sample09 samplei2 samplei5 samplei8 sample21 sample24 sample06 sample09 samplei2 samplei5 samplei8 sample21 sample24 Help Save Save s lt Back Flexchip Handbook 24 5 Method Editor f 500ul min 10 3f Z2 amp 8 AXIS 500 ul min 12 Mouse IgG
17. IPump Calibration Point 1 Enter Measured Volume in microliters l ET OT Chip Drawer Control Progress It pu Qut Z Chip in place Save Result Z Calibration Type Selected Z Buffer Line Primed pE Z Calibration Point 1 Measured Z Calibration Point 2 Measured Factor Calculated Cancel Ready for calibration Select Calibration EXE Prime Buffer Line Valve Position 1 to Chip Prime Line Now Calibration Point 1 v Use Recommended Settings Enter Measured Volume in microliters 0 12 Calibration Point 2 V Use Recommended Settings Stat Pump 2 Enter Measured Volume in microliters 0 125 Buffer Pump Factor rpm ul min 2352 3 Pump Offset RPM 2 9411754 Flexchip Handbook 10 85 Buffer Pump Factor rpm ul min Pump Offset RPM Buffer Pump Factor 0 06 0 14 Pump Offset FOTS HADISI Sy 2 7y S 4 L C BENY 07 2
18. V 500 10 BE Flexchip Control Software Flexchip System Flexchip System Method Editor mun G M Wash Prime 7 Load Options ichs pe GER REA Wash Sample Paths After Injection Perform wash Before Run Perform Wash After Run Total program duration 0 00 minutes Method Editor Analysis Temperature C 25 0 20 37 l Flexchip Handbook 5 25 Dwner Read Only Locked by Owner Analysis Temperature 250 L K K K K K K K K K K K K RK K R U a Pre run Options Wash Prime Load Options Prime BuHfers Before Run 7 Wash Sample Paths After Injection Perform Wash Before Run Perform Wash After Run Total program duration 0 00 minutes Pre run Option OLIChip Flush 1 Blocking Buffer Cycle 3 Pause 5 00
19. iB OANA HE SP gt lt e5X33J2 lE Ty
20. Please insure that buffer line 1 is in a container with at least 200 ml of fluid Press OK when buffer line 1 is in place i Milli Q 200ml 1 OKI Flexchip Handbook 82 Progress Z Chip in place i Z Calibration Type Selected ES Ready for calibration 2 Buffer Line Primed 3 Calibration Point 1 Measured 3 Calibration Point 2 Measured 3 Factor Calculated Save Result Select Calibration Calibrate New Tubing Hgpalibrate Old Tubing K s D 5 D s 5 S 5 P 5 SE S 5 5 S Prime Buffer Line Valve Position 1 to Chip E Prime Line Now Already Primed KK K K K PD E T Z Calibration Point 1 Use Recommended Settings Start Pump RPH 50 Time sec Enter Measured Volume in microliters Calibration Point 2 v Use Recommended Settings Start Pump RPM 150 Time sec 120 Enter Measured Volume in microliters Buffer Pump Factor rpm ul min 0 12 Pump Offset RPM Prime Buffer Line Value Position 1 to Chip Prime Line Now Please place a collection tube under the calibrat
21. Use Mouse to Create ROIs amp 1 ROI NN Method Run C VFLEXCHIP 2wethodsxTraining Kit spr oT X Method Setup Buffer Setup Sample Setup X Chip Setup X Run Prepare Fluidics Status Fluidics System is Ready for Run Fluidics Prep is complete Single Image Acquired Load and Prepare Chip Clamp Time remaining Jog Bypass Jog X Chip has not been loaded Approve R IMatri ROI Matrix Settings ROIMatrix has not been created 99 89299 Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute Chip has not been filled 6 99199959 gt 999 9 e e 2 9 6 e 0 e o 9 2 Source Angle Image Update 19 007 m Continuous Am sn lil Method Run C FLEXCHIP 2WethodsxvTraining Kit spr Q F M Method Setup Buffer Setup Sample Setup XX Chip Setup X Run Prepare Fluidics Status Fluidics System is Ready for Run Fluidics Prep is complete Single Image Acquired Load and Prepare Chip Clam
22. Flexchip Control Software The first step in the method must be of type Buffer Read Only Locked by Owner Analysis Temperature C 250 Pre run Options Em Wash Sample Paths After Injection Blocking Buffer 3 59 I Wash Prime 7 Load Options Perform Wash amp fter Run Chip Flush 2 Cycles Pause min Total program duration 0 00 minutes BENHEHNEHENHEHNHEHEHEEHEHHEHEHEHEHHEHHEHEHHEHEHEEHEHHEHEHEHEHEHEHEHEHEHEHEHHEHEHEEHEHEHEHEEHEEHEHEHEHEHEHENH Buffer Selection JU 4 77 7 a x b Running Buffer l Flexchip Handbook 5 27 Read Only Locked by Dwner Analysis Temperature C 250 Pre run Options Wash Prime Load Options V Chip Flush 1 epiac E Tn Buffers Before Run Wash Sample Paths After Injection Perform wash Before Run Perform Wash After Run Chp Flush 2 Cycles Pause min unmumuumumunmunmnununununumuununununuununuuunumunnuuunnmnununununu Total program duration 20 00 minutes BEHENEBENHBEHNEHNHENHEHHEHEHEHEEHEHEEHEHEHEHEHEHEHEHEHEHBEHEEHEHHEEHEHEHEHEEHEHEEHEHHEBHEEHNH Flow Rate ulmin 500 RU Duration min 10 100 2000ul min 0 10 600 00
23. 3 Completed Analysis Export Table Flexchip Evaluation Software CAFLEXCHIP acd Fie Chip Layout Completed Analyses Result File Properties Group Name Analysisome Date Created Medel Status ka l Ms kd 1 s 1072172006 2 23 05 PH 111 binder Passed 1417045 2006 2223206 PM 1 1 bevdng Passed 1 100645 IEEE 2 234056 1 1 bandi 1 13185 OEO kal l Ms kdi l s ka2 l RUs U 36M 2 66000 16 4 3 55004 3 0006 9 3 1426 Exjxorl Tables Flexchip Handbook 71 72 9 9 2 1 2 OFF 2 9 1 VP6 Milli Q 4 Flexchip Control Software 4 25 30ml Milli Q lt
24. Start Run Just in tIme Sample Loading Just in time Sample Loading Please prepare sample set 1 place the sample carrier in the instrument and press the button before the progress bar gets to 1002 Time before samples are required 18 minutes remaining Sample Carrier is Ready Set 1 Details Set Detaills Flexchip Handbook 7 51 Sample Set 1 Details Please insert a loaded sample carrier SP1 Atleast 1 6 mL of 50 nM Mouse 196 5 2 lt not required gt SP3 lt not required gt Flexchip Carrier is Ready Time before samples are required Method Step1 lil Method Run C VFLEXCHIP 2wethodsxTraining Kit spr iu TET de 3 15 dc 7 v Bi Z X Run Result File ROI Information Method Progress Selected Options Events Flow Rate L min 500 00 Duration min
25. 8 4 Summary Plot Completed Analyses Summary Plots R Summary Plot 1 9eRemaining Summar y C MELEXCHIP Watak Traintg acu a Plots Summary Table Mises vt iem Group Nanas mE B 90 Goremaining 100 tty crt binding _ late binding_late Flexchip Handbook 8 67 2 On off rate Plots Surnmary Table rabie Zoom ka ka Kp k k gt 0 100 pM 1nM 10nM Rm 1 HM QAI a 10 uM s Au pd 11 1 8 MA AMT 4 3 2 414 0 log
26. gt Flexchip Control Software Configuration Instrument Maintenance Configuration System Scan Parameters Instrument Maintenance Tubing Dimensions Fluidic Parameters User Peristaltic Pump Calibration Factor Offset Most Recent Calibration Buffer Pump 012 0 Calibrate 09 19 06 15 26 25 Sample Pump 011 0 Callbrate 09 19 06 15 52 58 s 4 Idle Method iw Run ldle Method Method CAFLEXCHIPMMethodsNWwaterFlushl m WH After system has been idle for 240 minutes Ku L Miscellaneous Syringe Maintenance Needles Maintenance System Purge Run Idle Method After system has been idle for 240 360 Flexchip Handbook 9 73 9 2 gt Desorb Kit BR 1008 23 BIAdesorb Solution 1 BlAdesorb solution 2 Milli Q 100ml 709 50ml lt gt 88 109m l 70 System Purge System Purge l
27. 50ml E i P ti 3 IVA UBA II Li s 23 E Falt E Buffer to degasser port 1 7 2 Flexchip Control Software Flexchip System Chip Definition Method Editor Method Run E valuation Flexchip System Method Run Open Method Method l Flexchip Handbook 36 7 Method Run C VFLEXCHIP 2WethodsxVIraining Kit spr F X Method Setup X Buffer Setup Sample Setup XX Chip Setup Run Method Name ITrainina Kit ich a m dministrator mmu Comments Analysis Temperature m Method Value 25 0 Apply Now Bl Current Setpoint 25 0 Current Temp 25 0 E Wash Prime Load uM MS MS DU D EU musasasa Pre run Options Chip Flush 1 Cycles Pause min Blocking Buffer X 3 5 0 Chip Flush 2 Perform Wash Before Run Prime Buffers Before Run wash Sample Paths After Injection Perform W ash After Run Cycles Pause min Flow Rate uL rnin 500 00 500 00 500 00 Duration min 10 00 12 00 10 00 Operation Buffer Selection Recirc Buffer Set 1 Sample 1 Buffer Running Buffer Y L1 Running Buffer Y
28. 500 12 00 Read Only Locked by Dwner Analysis Temperature 25 0 Wash Prime Load Options Iv Chip Flush 1 Cycles Pause min Prime Buffers Before Run Wash Sample Paths After Injection Blocking Buffer 3 so Perfom Wash Before Run Perform Wash After Run ChipFlush 2 Cycles Pause min Fs ss Step3 O0OIBRCGIE tL29 Tv ZX u BmBU7r rv AZLRDE Operation Buffer Buffer Selection Running Buffer 500 10 000 l Flexchip Handbook 5 29 Save SPR Method Save In CO Methods 2 E Getting Started Kit spr amp Sample Inject Test spr My Recent Documents My Network File name Training Kit spr M Flaces Save as type SPR Method spr v Cancel Save Method C FLECHIP2 Methods Method 2 Save Method Run with this Method Flexchip Handbook 30 6 6
29. 1 4 Pre Gasket Window Top Section Pre Gasket Window Flexchip Handbook 6 33 Top Spring Plunger 1 4 Pre Gasket Window Top Section Bottom Section Flexchip Handbook 34 6 5 Top Section Top Spring Plunger 1 4 Top Section Bottom Section Front Side Spring Plunger 1 4 Flexchip Handbook 7 35 7 Method Run 71 6 6 way 5
30. Configuration System Scan Parameters Instrument Maintenance Tubing Dimensions Fluidic Parameters User Peristaltic Pump Calibration Factor Offset Most Recent Calibration Buffer Pump 012 0 Calibrate 09 13 06 15 26 25 Sample Pump 0 11 Calibrate 09 19 06 15 52 58 Idle Method Run ldle Method Method CNFLEXCHIPNMethodsNW aterFlushl idle zm After system has been idle for 240 minutes EE m Miscellaneous 1 Syringe Maintenance Needles Maintenance System Purge Needles Maintenance PENA izadia Please remove the sample tray then Progress press Next 3 Sample Tray Removed 3X Needles Lowered 3X Needles Replaced 3X Needles Raised Cancel 15 Flexchip INextl l Flexchip Handbook 76 10 112319 Progress Replace needle parts as required then Z Sample Tray Removed press Nest Z Needles Lowered 3 Needles Replaced 3 Needles Raised 709 Mill
31. 55 55 Flexchip Handbook 8 2 Ka ka Kp 59 8 3 es 64 8 4 Summary Plot kk 66 8 5 68 9 8IGIN DO Se 68 8 5 2 UO T 2 Evene ead eoim ese odio 69 es 72 SR MES MI rdi cii 72 92 m Au X ET Qha 73 oo 74 10 1 74 2 SADA AT S ER p o m 76 1038 wg dg wA edm Lr u uuu 80 10 3 1 80 10 3 2 0 85 11 System Check i 91 Flexchip Handbook 1 1 1 Flexchip Chip Definition Method Edition Method Run V Evaluation 1 2 Flexchip Ir
32. 50ml Flexchip Handbook 3 9 1 1 1 Mili Q 1 VP6 6 pdi 6 Begasser gi A port 1 Bufferto port5 ra 2 port 1 A Flexchip Handbook 10 3 3 2 System Purge Flexchip Control Software Configuration Instrument Maintenance z Flexchip Control Software Flexchip System Chip Definition Method Editor Method Run Tali Configuration Configuration System Scan Parameters Instrument Maintenance Tubing Dimensions Fluidic Parameters User Peristaltic Pump Calibration Factor Offset Most Recent Calibration Pump 012 Calibrate 09 19 06 15 26 25 Sample Pump 0 11 0 Calibrate 09 19 06 15 52 58 Idle Method Idle Method Method CFLEXCHIPAMethodsNWaaterFlushl idle P After system has been idle for 240 minutes HENENEENEENENEHNENENHNEHNEHNENEHNENEHNEHNENEHNEHNENENEHN
33. Calibration Point 2 Use Recommended Settings Start Pump Calibration Point 1 Enter Measured Volume in microliters l Powy Baloratu Chip Drawer Control Progress Save Result D Out Z Chip in place _ Save Resul Z Calibration Type Selected Z Buffer Line Primed n Z Calibration Point 1 Measured Z Calibration Point 2 Measured Z Factor Calculated Cancel Ready for calibration Select Calibration EE Prime Buffer Line Valve Position 1 to Chip Prime Line Now Calibration Point 1 Use Recommended Settings Enter Measured Volume in microliters 0 12 Calibration Point 2 Use Recommended Settings Stat Pume 50 2 Enter Measured Volume in microliters 0 129 Buffer Pump Factor rpm ul min 2352 3 Pump Offset APM 2 341 1764 Flexchip Handbook 90 10 Buffer Pump Factor rpm ulmin Pump Offset RPM Buffer Pump Factor 0 06 0 14 Pump Offset FOTS
34. Analysis Temperature Method Method Value IApply Now Current Setpoint Current Setpoint Current Temp Method Setup Z Buffer Setup l EA Method Run C AFLEXCHIP 2WethodsVIraining Kit spr d S Method Setup XX Buffer Setup A Sample Setup AX Chip Setup X Run Available Buffers Buffer Name Running Buffa 48 3 Deionized wa Deionized Wa m Blocking Buffe Assign to Valve Position 1 18 0 Assign to Valve Position 2 Assign to Valve Position 3 Assign to Valve Position 4 Assign to Valve Position 5 Assign to Wash Valve Position Assign to Selected Valve Position Add New Buffer s Delete Selected Buffer Import Export Properties Flexchip Handbook Current Buffer Assignment Installe d Manual Each buffer used by the current method must be assigned to a valve position and primed before the method can be run Right click in either list to see a menu of operations that can be performed in the respective list Double click on an available buffer to assign it to the currently
35. Mili Q BIAdesorb Solution 2 System Purge 4 Milli Q System Purge Milli Q System Purge BIAdesorb solution 1 Flexchip Handbook 80 10 10 3 3 2 gt Milli Q zk 200ml Sample Tubes 5ml BR 1007 06 f EXER 1 000 10 0000 lt gt 10 3 1 Configuration Instrument Maintenance Configuration System Scan Parameters Instrument Maintenance Tubing Dimensions Fluidic Parameters User KOR K R K K KRK R RK KE REESE R SE K KE alibration Factor Offset Most Recent Calibration Buffer Pump 012 0 Calibrate 03 13 06 15 26 25 Sample Pump 011 0 Calibrate 09 19 06 15 52 58
36. 159 3 176 7 Z Batch Export Export Print Export Flexchip Handbook 70 8 Exporting Sensorgram Export MetaFile C BMP C JPG C PNG C Test Data Only Export Destination ClipBoard C File C Printer No Specific Size Millimeters C Inches Points Cancel width E 000 818 Units Printer Export Sensorgram Export Sensorgram Select Subsets and Points All Data Selected Data Subsets to Export Export what C Data Data and Labels Data to Export C Esport Style List Table Delimited Row vs Column C Points to Export Tab Comma Numeric Precision Current Precision C Maximum Precision Cancel 2 Summary Plot Plots Export plot Summary Table Export Table Flexchip Handbook 8
37. Protein Protein Protein Pnb aim AJ 1 1 12 1 1 4 D 12 1 Spot types Background IZ 1 8 5 12 8 Background Background BSA Signal Protein A G Flexchip Handbook 18 4 4 2 Chip Definition Manual Save As Save chip definition As Save chip definition As Enter chip barcode 0200001329 Existing definitions Help Cancel Enter chip barcode 10 OK Method Run gt ChipDefinition The item 0200001329 already exists Are you sure you want to replace it Yes Mo 4 8
38. ISave As Save chip definition As OKI Protein AG Protein Protein Protein Protein Protein A G Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein 19 Flexchip Handbook 20 4 44 Tab Chip Flexchip Chip Definition Software Create new chip definition Import tab delimited text file h W Text file import settings Import file Browse Spotter configuration Number of rows on chip H Fill by row Select spotting direction on chip Number of columns on chip gt C Fill by column SENT ange chip orientation Number of replicates for each sample f H Number of required samples in file 48 Flexchip configuration M
39. Web 2009 GE St lt GE 169 0073 3 25 1 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com ISO 9001 2000
40. Define chip manually Export chip definition to file e Existing chip definitions Open chip definition amp 7 7d Flexchip Handbook F Chip Definition Chip bar code 0200001329 2 29 8 Chip orientation Number of columns Show concentrations Protein Protein JG Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Number of rows 4 Open chip definition Select barcode 0200001329 Help Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein DK Cancel Spot types R Signal Backaround Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein Save
41. EZ EN Flexchip Handbook 60 8 ftr Calculattons Settings Concentration 50nM Linear drift correction Off Model 1 1 binding lt gt Flexchip Evaluation Software 2 1 1 binding 1 Ka A B AB ka Bivalent analyte 2 2 AB AB 2 Ka A B AB Kaz AB2 Ka2 A B Flexchip Handbook Calculating Results Protein 25 Protein 50 Protein
42. 100 Fie Hep M Calculations Report Point Table Report Point Ftot bare ne pot ka 1 m amp Ed 1 6 KD M Ran RU Cone 141 141245 2 Nrie 9 TAi EE LE ms 86 1 5871 4 1075 0 918 2 LOCO T 784 5 5 DOC Passed Calculating U value Pending 8 61 Accept Curent Abort Current Abort Remaining Fitting Status Passed Protein AG 2 Fiting passed immeriahon bl nid eormechon eom How ub min kt RU Ms Td T Rmax 1 8 SER 1 8 RU XI 2 015324 13 1 96824 13 1 2008 03 7 4 084 ED 25e 03 1 454803 PE B Tet EN 1 1 5 Gnd ii 884e4 13 IF 1 953e 13 1 54024 13 z ZI 1107 64 5 174 5 164 4 113 4 VR 210 2 201 2 UC ZU 219 4 157 3 193 2 173 8 B5 12708 4 345 4 484 27 70 A E T 7 u 770 7 Repot Flexchip Handbook 62 8 ka ka Kp
43. X Chip has not been filled Source Angle Image Update 19 007 Continuous Apply Single Status Hh Rom m ma mm Ww lil Method Run C VFELEXCHIP 2wethodsxTraining Kit spr Fluidics System is Ready for Run Single Image Acquired e 99 6 79 929 929 9 6 99 06 282099 69 2902 59 Method Setup Buffer Setup Sample Setup X Chip Setup X Run 9 o 9 9 O0 v tv 9 0 9 Qo 99 OS a i 9 06 t 96 02 00 0 99 925 Pre Gasketed Window 15 Flexchip Handbook 7 43 Chip Information Identification Barcode 0200001 329 User Barcode Chip Type FLEXCHIP Pre spotted Affinity l ROI Matrix
44. 10 3 2 Configuration Instrument Maintenance l Configuration System Scan Parameters Instrument Maintenance Tubing Dimensions Fluidic Parameters User s alibration Factor Most Recent Calibration Buffer Pump 012 0 Calibrate 03 13 06 15 26 25 Sample Pump 011 0 Calibrate 09 19 06 15 52 58 a s s Idle Method Hun ldlg Method Method EMFLEXEHIPKMethadsWyaterFlush1 idle Cy n n After system has been idle for 240 minutes Miscellaneous Syringe Maintenance Needles Maintenance System Purge Cancel Help Sample Pump Calibrate amp 7 7 u 7 5 Flexchip Handbook 86 10 gawul Chip Drawer Control Progress Z Chip in place _ Calibration Type Selected B m m Ready for calibration Sample Line Primed Cancel 3 Calibration Point 1 Measured AERE EAE ERE M 3X Calibration Point 2 Measured Factor Calculated s Select Calibration Bi Calibrate New Tubing Recalibrate 019 Tubing B El Prime Sample Line Em Calibration Point 1 Calibration Paint 2 v ma NM Sample Pump Factor tpm ul min 0 11 P
45. Flexchip Handbook 8 65 Repot Point Table Report Point Plot Lu Fe Ede He finu Calculatioli Report Point Table 1 Point Plot K a w 5 pot w baseline DT rr ed FILIJ w se FLU s w bndeg HU w Spol Lone w babes HLI ww w mm Table Protein 3 Probein AX 10 Protein Aja 1 Probein 18 Probe 19 Probe PT Proben AS 3 Protein Proben Ms Proben Proben Ms Bi Protsin 53 Probein EE Proben AM amp T Protsin 74 Protein Probe BI Protsin ndn Protein a0 Probs BG 9 Protsin Apa 9 1917 3 755 I rm FnS 1 d 221 0 2000 2 EAB 2 TRIS ZO L i 201 1 H em zug 5 Ls y d HJ 2155 10 010 3 HIS Len 5 2013 3 2063 8 2150 2 2130 0 ZI 5 5 W analysis Pis Heb A s asna puu ddnmum Calculations Report Point Report Point EHEHEERHERR chum tede We Here pul hinding late RelResp RU Flexchip Handbook 66 8
46. Number of columns 12 l W Chip Definition Manual Chip orientation RETURN BE Spot types Number of columns B ES g Signal Background mus W Show concentrations Content Content Show concentrations Conc Flexchip Handbook 16 4 W Chip Definition Manual Chip orientation Maps ET BE Spot types Number of columns B ES m Signal Background Protein 4 6 50 Protein 4 5 50 Save Save s 1 1 Content BSA Conc 500 1 2 1 3 Protein A G Protein A G Protein AG l W Chip Definition Manual Chip orientation VERTES EN BE Spot types Number of columns fB m Signal Background Show concentrations 5 6 7 8 Conc Content Conc 500 Protien 50 Protien A G 100 Protien A G 100 BSA Paste Set spot type P Help Save Save s 1 1 shift 1 48 Flexchip Handbook W Chip Definition Manual Chip ori
47. Continuous Apply Single 12 Prep XFluidics Prep has not been run Fluidics Prep is complete Flexchip Handbook 42 7 dod S Prepare Fluidics Fluidics Prep is complete Load and Prepare Chip oor ua Jog Bypass Jog X Chip has not been loaded mom oa hn ROI Matrix Settings X ROIMatrix has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute X Chip has not been filled Source Angle Image Update d 2 13 007 Continuous Apply Single Status i T dr w B b EJ Method Run C FLEXCHIP 2WethodskxTraining Kit spr Fluidics System is Ready for Run v Method Setup Buffer Setup Sample Setup X Chip Setup X Run Image Acquisition Stopped Load and Prepare Chip O IOul oq S Prepare Fluidics Fluidics Prep is complete Load and Prepare Chip Clamp Time 4 minutes remaining BH gm mmu Jog Bypass Jog X Chip has not been loaded Approve ADIM atris ROI Matris Settings X R lMatrix has not been created imm Select Fill Operation Bypass Select Bubble Blast Buffer Execute
48. 20 00 2 Set 1 Sample 1 50 nM Mouse IgG 500 00 12 00 3 Buffer Running Buffer Fun 20 c ROI Map Real time Image Graph Scale e DD 9 o 06 9 9 9 9 9 o 9 9 OE e R ls colored by ROI Type Response RU 500 00 D 4 i Time min 10 00 Flexchip Handbook 52 7 Map ROI Show Reference Corrected Curves ROI ROI NN Method Run C VFLEXCHIP 2WethodsxTraining Kit spr T 3 j V t X V Chip Set Run Flow Rate Duration pL min min 500 00 20 00 2 Set1 Sample1 50 nM Mouse IgG i 500 00 12 00 3 Buffer Running Buffer 500 00 10 00 250 c Sensorgram reference corrected ROI Map Real time Image Graph Scale Q
49. 9 o o o ROI Matrix Settings X R lMatrix has not been created A I Select Fill Operation Bypass Select Bubble Blast Buffer Execute X Chip has not been filled o o o e o o o o e 9 o E o o o o O O O TQ X000 o w Source Angle Image Update 13 007 Continuous Apply Single Flexchip Handbook 46 7 Use Mouse to Select or Adjust ROIs amp ROI lil Method Run C VFLEXCHIP 2wethodsxTraining Kit spr F MX v Method Setup Buffer Setup Sample Setup XX Chip Setup X Run Prepare Fluidics Status Fluidics System is Ready for Run Fluidics Prep is complete Single Image Acquired Load and Prepare Chip Clamp Time ETE nns Jog Bypass Jog X Chip has not been loaded Approve R IMatris ROI Matrix Settings R lMatrix has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute Chip has not been filled Source Angle Image Update 19007 m Continuous Apply Single lil Method Run C VFLEXCHIP 2wethodsxTraining Kit spr oT MX v Method Setup v Buffer Setup Sample Setup XX Chip Setup X Run Prepare Fluidics Status Fluidics System is Ready for Run Fl
50. Content amp Concentration DK Fuexchip Evaluation Software C AFLEXCHIP 24DatakTrainig Chip Layout Completed Analyses Resut File Properties Tara Saensorgram Reference Connected ER BESA 500 Protein AG 25 Protemn AG 50 E Mk Protein AG 100 aint RU K d 1000 000000000000 amp amp amp amp amp amp amp amp amp amp amp amp amp amp amp amp amp amp amp amp EIIIIITTTTTT amp amp amp amp amp amp amp 000000000000 15 EU rriri mik v aadustmeni C Le amp columnrefenence comected daba F Rederence corected dala Non nederence cormecbed dala ROI map Non reference corrected data Flexchip Handbook 8 57 lt ZX gt 1 ROI map
51. Pump 0 11 Calibrate 09 19 06 15 52 58 Idle Method iw 19 Method Method 1C SFLEXCHIP Methods Ww aterFlush1 idle p After system has been idle for 240 minutes EE Miscellaneous gs a Syringe Maintenance Needles Maintenance System Purge System Purge Fir Z ES Progress The System Purge operation is used to fill the fluidic paths in the system with a single fluid or air 3X Insert Chip 3X Select Valve Positions Prepare Valve Positions 3 System Purged Press Next to continue System Purge INextl Flexchip Handbook 18 10 Pirsz 3 Progress chip is required for the System Purge operation Insert a chip using the In and Out buttons 3X Insert Chip 3 Select Valve Positions Chip Drawer Control 3 Prepare Valve Positions uc Qut 3X System Purged i Cancel Flexchip Out Inj l zata Fir Z Progress Select the valve positions to be included Sustern Purge Valve positions 1 and are required Z Insert Chip 3 Select Valve Positions Select the valve
52. a menu of operations that can be performed in the respective list Double click on an available sample to assign it to the currently selected sample position Available Samples Available Samples Add New Sample s Define New Sample Sample 5 nM Mouse IgG Close Sample Description Number of samples added Define New Sample Sample Name 50nM Mouse IgG Add Close l Available Samples 50nM Mouse IgG Assign to Set 1 Position 1 Flexchip Handbook 40 7 MN Method Run C AFLEXCHIP 2WMethodsiTraining Kit spr d Hb o LET TME mis ua mu s C _ g vabi Samples Assigned Sample Vol mL ze Test Solution 1 5 1 ESSE 1 6 Test Solution 2 Water SP2 50nM Mouse 196 10 10 2006 SP3 U Set 2 EE i SD r sss s 5 2 5 3 Set 3 5P1 SF2 ES Set 4 SP1 SP2 SP3 Sample name assignments have been successfully completed
53. w log k 10 pM Flexchip Handbook 68 8 8 5 Flexchip Evaluation Software JPEG 8 5 1 Batch Export Settings TTTTrrrmhrrrrrrrrrlrrrrfrr TT TT TT Fesp bare ne RU 8 3 E 9 40 T s fani Shr kn kil Shena ratu Spot ka qayma kd 1 4 Rmax RU Cone M Flow ut nun kt RU M RU2 T Rmas st ka 1 94 1 8 5EORmax Ru Tz 141245 3766 4 2 NDNe 3 Sn 2 zaal 10 7 64d 5 191 E O6 4 545 4 484 4 571 4 64 4 401 4 578 4 842 1TH 4 671 4 654 4 522 Calculations Batch Export h Flexchip Handbook 8 69 Browse For Folder Sensorgram data and graphs for all analysis groups will be exported to a Folder under the selected Folder B My Documents 4 Computer A 31 Floppy A Local Disk C C Bia Users COMPAQ 2 Cpqapps C
54. 0 Raw 7 8 9 00000000 10 1 11000 12 000 BA 7 Background 25ug ml Signal BSA 200ug ml Em BSA 200ug ml BSA 200ug ml BSA 500ug ml Background Flexchip Handbook 14 4 4 1 Flexchip Control Software Chip Definition Windows XP Start BIA Programs Flexchip Chip Definition Software 2 0 Flexchip Control Software Flexchip System Create new chip definition Definition chip manually i Flexchip Handbook 4 15 W Chip Definition Manual Spot types Chip orientation mE E a mllumber of rows L Signal Background mumber of columns B E igna E ackgroun m Show concentrations Help Save Save s Chip Definition Manual Chip orientation rows columns Number of rows 8
55. 1 02 03 04 05 06 BSA 01 02 03 04 05 06 BSA 01 02 03 04 05 06 BSA sampleO01 02 03 04 sample05 sample06 BSA sample01 02 03 04 05 06 BSA 01 02 03 04 05 06 BSA 01 02 03 04 05 06 BSA 01 02 03 04 05 06 BSA 01 02 samplen3 04 05 06 BSA 01 02 03 04 sample05 06 BSA lt Back Close 2 96well A1 12 B1 12 24 4 Text Well ld Content Conc Well ld Content Conc A1 sample01 100 A2 Sample02 100 sample03 100 A10 sample10 100 A11 sample11 100 A12 sample12 100 B1 sample13 100 B2 sample14 100 B11 sample23 100 B12 sample24 100 Flexchip Handbook 4 23 Spotter configuration
56. 58 36 Evaluation Date 10 24 2005 2 58 45 25 0 C system check 20061074 acd 0 9510 1 095 z 80 RI 2 7 ROI lt 55 RU 227 f1E3 tn 1950 7510 75 RU 55 RU 227 z 3 0RlI 22 1125tn 1125 Rh 2 510 125 0 5to 40 RUM 20 ROIs 0 17 24 9510 25 O05 C z 8 C 25 2C 18 510 19 5 10 0510 0 3 0 910 1 5E Narning Ambient temperature should not change faster than 2 C h The temperature change during this run was at one point in time 3 1 C h This may cause decreased instrument performance Fail Fail 2 9 b 556114 1097 UC A Flexchip Handbook rO ERROA LU EIB PE OREORE A coUv duUlcla 151 89 ms sess CES rine _ E vm i IC 525 576 D DR
57. D Documents and Settings C3 FLEXCHIP C3 FLEXCHIP 2 C Data CO Diagnostics v 1 Cancel B E E EH Graphs Data JPEG 8 5 2 1 Analysis Calculations Report Point Table Report Point Fiot Protein A G 25 Settings Fiting passed nmiCrrlrahn im bl nid eormechon bare ne E E 8 Bide Data Labels Mik Daka 5 Mimie Zamka F Showkak kil r mr aal pounds saas Spot ka 1 M4 kd 1 6 KD M Rman RU Cone M Flos ut min kt Rums U RU2 Ta T kd Toman St ka 1 9 1 9 St mas 1 417845 2 sag 5 e 5n 2 zac 1132 66 26 1252 z T YT T 121 8 I 181 5 A ati T 11 pos 5 3 515610 185 2 527 1 199 1 961 9 24d Iss 042 5 7 508611 HH 4232 TTE 195 1 gera 7 3X eel 210 2 1027 4 704 5 319 2 5 233011 201 4 9 B goes 1965
58. EREREERERERERRREERERERERERERRERERERERN e e ee 00999 e e 8 O o e e e B Spotter configuration 600 00800900 Number of rows on chip 12 C Fill by row Select spotting direction on chip H Number of columns on chip Fill by column s Change chip orientation oo ee Number of replicates for each sample 12 Change start position Number of required samples in file 8 EE REEEEHENHNEENN EEBHNEEHRBEHEHNEHEHHEHNEHEHEHEEHHEHEEHHEHEEHREEEHREEHREEHEHREBEEHEEHEREEEN Flexchip configuration Columns 8 format and chip orientation in Flexchip Import options File requirements Read samples in listed order Content Sort samples by wellid Source plate size Sample 10 mM Sample B 20 mM Sample C mM Required Optional Sort direction Help Preview gt Cancel Next gt Flexchip Handbook 22 4 F Chip Definition Text import C Documents and SettingsMAdministratnrMDesktnnX0200001 3 l BR Chip orientation TOS ESAE f2 Spot types Number of columns e BH Signal Background Show concentrations 2 3 4 5 6 7 sample01 02 03 04 05 06 BSA 01 02 03 04 05 06 BSA sampleO0
59. GE Healthcare 3 Biacore Flexchip Instrument Handbook GE imagination at work MEG 3 I 3 3 13 8 K UKU 7 FD x O ES al ca E Re u i dra oe 4 Fp ua e G PPR Gi sS s s 4 1 3 2 i 5 2 En o A VDE S i 6 2 1 DE HRQ E 6 DEP 6 23 vv Z fftQ OE 6 7 3 1 6 6 Way JUL 70 7 3 2 System Purge kk 10 4 Chip Definition 13 4 1 14 42 kk 18 4 3 18 4 4 20 5 Method Editor 24 Mou rU mA riv EET 30 7 Method Run a a 35 71 X 35 CR 35 8 Evaluation
60. HNENENENHNENENENENENENENENEN m Miscellaneous m B Syringe Maintenance Needles Maintenance System Purge B m System Purge Flexchip Handbook 3 11 Progress 3X Insert Chip Select Valve Positions 3X Prepare Valve Positions 3 System Purged The System Purge operation is used to fill the fluidic paths in the system with a single fluid or air Press Next to continue System Purge INextl r Progress 3X Insert Chip 3X Select Valve Positions 3X Prepare Valve Positions 3X System Purged chip is required for the System Purge operation Insert a chip using the In and Out buttons Chip Drawer Control Flexchip Flexchip Handbook 12 3 E Select the valve positions to be included in System Purge Valve positions 1 and 5 are required FF SET murza Progress Z Insert Chip 3 Select Valve Positions Select the valve positions v Valve Position 4 Prepare Valve P
61. Right click in either list to see a menu of operations that can be performed in the respective list Double click on an available sample to assign it to the currently selected sample position Assigned Sample Name Set 1 SP1 50nM Mouse IgG 1 6mD Method Sample Setup Chip Setup NN Method Run C VFLEXCHIP 2WethodsxTraining Kit spr MX Dia sar m W Method Setup Buffer Setup Sam Setup XX Chip Setup X Run m Prepare Fluidics ars Bi Begin Prep Bypass Prep Fluidics System is Idle B o c rr wm X Fluidics Prep has not been run Intensity 1147 4 629 Clamp Time Complete Jog Bypass Jog X Chip has not been loaded Approve R IMatrix ROI Matrix Settings R lMatris has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute X Chip has not been filled ES D LEGUUD DEUXPEQUIASIQ S MUTET SCVV E Tie c Source Angle Image Update 19007 m Continuous Apply Single Flexchip Handbook 7 41 Prepare Fluidics Begin Prep
62. Run 2WMethodsxTraining Kit spr HM 4 Method Setup W Buffer Setup W Sample Setup X Chip Setup X Run Prepare Fluidics Status Fluidics System is Ready for Run Single Image Acquired 4 Fluidics Prep is complete Load and Prepare Chip Clamp Time Complete ES 22 Chip is ready Approve R IMatrix o OUO 6 0 e o 6 ROI Matrix Settings R IMatrix is Ready B WUNRSZNHUR HON o o9 0950 100 G SG o o o e o e e e 9090 Fill Options Select Fill Operation IEipfuh Select Bubble Blast Buffer Execute X Chip has not been filled m m 8 19 007 Continuous Apply Single o o to CI e 5 99 mmmu jm gt w Fill Options Select Fill Option Chip Flush Execute 18 l Flexchip Handbook 48 7 lil Method Run C FLEXCHIP 2wethodsxTraining Kit spr HM 9 4 Method Setup Buffer Setup Sample Setup X Chip Setup X Run Prepare Fluidics Sta
63. Selected Data Subsets to Export Points to Export Flexchip Handbook Export what Data Data and Labels Data to Export Esport Style List Delimited Tab Numeric Precision Current Precision Maximum Precision S Sensorgram Table Row ys Column C C Cancel 8 55 8 Evaluation 8 1 Flexchip Control Software Evaluation Windows XP Start BIA Programs Flexchip Evaluation Software 2 0 Flexchip Control Software Chip Definition Method Editor Method Run Evaluation Open Results File Fie Name Path Opened Time Opened Help Browse IBrowse Flexchip Handbook 56 8 Create Groups Create analysis groups based on matching entries for Content amp concentration 4 groups C Content 2 groups Help Cancel Content Concentration Content
64. XTrainig Kil acd Fie Chip Layout Completed Analyses Result File Properties Analysis Mame Created a Model Status ka l Mis kd 1 s KD M kal l Ms kdi i s ka Rush kd2 1 s U Cha muy Mr 1 1071512006 6206509 AM Mni binding PFmrred 1 10 15 2006 6 05 10 lil bidrag Parsad IZ erS 347264 1 OE 6 S dO ET 1 1230 58 Flexchip Handbook 64 8 8 3 Calculations Show report point Settings e 8 m esp bare ne pr epi 12 zt 9 Tina iri x dm G G m G mG s s s G G Cakulsie Reus For Al Groups ns rear Zewnkxk i X dod rega h She repr s Spat ka 1 9 kd 1 5 ED M Rmas RU Conc M How uL min kt RI Ms RN W f fam m EG tE Window Initial reference Baseline Baseline 130 120 sec 120 sec reference 100 100 100 100
65. aining Kit BR 1007 22 Pre Spotted Affinity Chip 1 Pre Gasketed Windows 1 Rat lgG2a analyte 1 Flexchip Blocking Buffer 10x 250ml BR 1007 08 HBS EP 10X Buffer 1000ml BR 1006 71 Sample Tubes 5ml BR 1007 06 Desorb Kit BR 1008 23 10 200ul 1 5mD 50ml 500ml 50ml Milli Q 2000ml 70 100ml DD gt Flexchip Handbook 4 1 1 3 1 3 1 Power on LED Power on LED Pump compartment lilii m Power button Chip drawer Buffer bottles Bulkhead unions Sample carrier Flexchip Handbook 1 5 1 3 2 Flexchip Instrument Control Windows XP Start BIA Programs Flexchip Control Software 2 0 SE Flexchip Control Software Flexchip System Chip Definition Method Edito
66. atris format and chip orientation in Fleschip Import options M File requirements u Read samples in listed order Content Conc C Sort samples by wellid Source plate size Sample A 10 mM Sample B 20 mM Sample C mM Required Optional Sort direction G m Nd CC SN aaa aw W 2 PIE IE EN 1 B1 C1 SN PS x Lw uw yw UAS TASS ww w as Help Preview gt Cancel Browsej Flexchip Handbook 4 21 1 96well 8 12 Text Content Conc lt Content Conc Tab Sample01 100 Sample02 100 Sample03 100 Sample04 100 Sample04 100 Sample05 100 sampleO6 100 sample07 100 sampleO8 100 Spotter configuration W Text file import settings C Documents and SettingsWdministratorXDesktopX0 200001 329 1 3 4 5 6 T 8 Import file eee 6 O O 6 e 8 5B O Oo 9 6 6 6 68 O O 6 6 6 F Documents andSetingsVAdminisatoADesktopV2ODQOT32800 Browse FBESEHREERERERER
67. e e o 2 n SJ 3 N 1 b 5 Response RU cen 99eeeeeeem 99eeeeeeoee ST SD e p 2222022008688 ee e e o o p R ls colored by ROI Type Time min E Method Run 2WethodsxTIraining Kit spr d J Method Setup Buffer Setup Sample Setup AX Chip Setup X Run Result File ROI Information Method Progress Selected Options Events Step Flow Rate Duration pL min min EIUS T 2 Set 1 Sample 1 50 nM Mouse IgG 500 00 12 00 3 Buffer Running Buffer 500 00 10 00 seme ROI Map Real time Image Scale e CECI amp e e amp OEO eo o o o e o S amp amp amp eG emi Respanse RU ROls colored by ROI Type 20 Time min Flexchip Handbook 7 53 Run Ended OKI Run Ended Cornpletion Status Method ran to completion Saved CAFLEXCHIP 2AData Trainia Kit acd Performing Wash After Run Additional output files for this run may be saved by clicking the Save icon on the Result Fil
68. e tab Method Perform Wash After Run VP6 Milli Q 9 4 1 5 6 VP6 lt gt Run Export Print Exporting Sensorgram Export MetaFile C JPG C PNG C Test Data Only Export Destination ClipBoard C File Printer No Specific Size Millimeters Inches Points Cancel width 1000 818 Units Printer Flexchip Handbook 54 7 Export Export Sensorgram Select Subsets and Points C
69. entation Mer al IUE HE Number of columns B Show concentrations 1 2 3 4 Content Conc Content Conc Content Conc Content Conc Content 500 Protien 25 500 Protien Aja 25 Copy Set spot type P lil Help Save Save s 4 Spot types E Signal Protien Aja 25 Protien Aja 25 Background Protien A G 50 Protien A G 50 Protien 50 Protien 50 17 24 Paste 3 1 W Chip Definition Manual Chip orientation Number 12 Number of columns 8 gt Show concentrations Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein J Help Save Save s Spot tupes Signal Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein aim A Backaround Protein Protein Protein Protein Protein Protein Protein Protein Protein Protein AJ Protein Protein Protein A G Protein Protein A G Protein Protein
70. gt On gt m EILAN QU 5 35 5 3U 977 0u 3 JALAN 177510 Ab IC 60 a5 2 B S B 2 Fisch CRUS VN i x
71. i Q OKI Buffer 6 gt SPR Please accurately measure in microliters the amount of fluid collected in the tube at the calibration port and enter the amount on the form OK Milli Q ul 1000ul 19 Enter Measured Volume in microliters Flexchip Handbook 10 89 awula 1 3 Chip Drawer Control Progress Qut Z Chip in place Z Calibration Type Selected Z Sample Line Primed Z Calibration Point 1 Measured 3 Calibration Point 2 Measured 3 Factor Calculated Cancel Ready for calibration Select Calibration sio Tubing Prime S ample Line Prime Line Now Calibration Point 1 Lise Recommended Settings Enter Measured Volume in microliters 0 12 umum g Calibration Point 2 Use Recommended Settings Start Pump al 1 Sample Pump Factor 0 11 Pump Offset RPH
72. i Q 3 Next Progress Needle change maintenance complete Sample Tray Removed Needles Lowered Needles Replaced Z Needles Raised 5 Flexchip IDone 10 2 1 Milli Q PM BlAdesorb Solution 1 9 BlAdesorb Solution 2 4 Milli Q System Purge 100 gt Desorb Kit BR 1008 23 BIAdesorb Solution 1 BlAdesorb Solution 2 Milli Q zk 100ml Flexchip Handbook 10 2 lt gt 1 Milli Q System Purge Configuration Instrument Maintenance l Configuration System Scan Parameters Instrument Maintenance Tubing Dimensions Fluidic Parameters User Peristaltic Pump Calibration Factor Offset Most Recent Calibration Buffer Pump 012 0 Calibrate 03 13 06 15 26 25 Sample
73. ion port Press OK when the collection tube is in place Flexchip Accessories Kit Pump Calibration Fitting Flexchip CAL I OKI Prime 2 l Flexchip Handbook 10 83 ruuu Guliuratiut Chip Drawer Control Progress B Z Chip in place Seve Besut Z Calibration Type Selected Cares Z Buffer Line Primed Calibration Point 1 Measured 3 Calibration Point 2 Measured 3 Factor Calculated Ready for calibration Select Calibration Prime Buffer Line Valve Position 1 to Chip ilium B Calibration Point 1 Use Recommended Settings _ Statt Pump 1 Calibration Point 2 Start Pump Buffer Pump Factor rpr ul min 0 12 Pump Offset RPH 0 Prime Calibration Point 1 Use Recommended Settings LERRET Start Pump Please place empty calibration tube at the calibration port Press OK when the tube is in place i Milli Q
74. it Pump Calibration Fitting Flexchip CAL I OKI Prime 1 Flexchip Handbook 88 10 pows Chip Drawer Control Progress Tn Dut Z Chip in place Save Pesut Z Calibration Type Selected Carica Z Sample Line Primed Calibration Point 1 Measured 3 Calibration Point 2 Measured 3 Factor Calculated Ready for calibration Select Calibration Prime Sample Line umum B Calibration Point 1 Use Recommended Settings Statt Pump 1 Calibration Point 2 d Start Pump Sample Pump Factor rom ul min 0 11 Pump Offset RPH 0 Prime Calibration Point 1 Use Recommended Settings LERT Start Pump l x Please place a sample tube filled with 5 ml of fluid in sample position 52 and an empty tube at the calibration port Press OK when the sample tube holder is in place i EL 5ml Milli Q S2 Flexchip Mill
75. le CNFLEXCHIP 2 Data Trainig Kit acd Acquisition Dutput Format Comments Save ACD File m m EEHEEHERHEHEHRHREHRHEHEHEHEHEHEHEHEHEHEHEHHEHEHEHEEHRERERERR W Start Prescan 250 Prescan s L ROI Map Real time Image Graph Scale ROls colored by ROI Type 19 degrees Result File Flexchip Handbook 50 7 Start Prescan 1 L Method Run C FLEXCHIPWethodsVlraining _ Dutput Format Save ACD File Start Run 25 0 T ROI Map Real time Image Graph Scale ROI ROI ROI ROI ROI Chip Setup Bubble Blast f 5 ROI
76. les awa ef a aha X Each ea rust be vale pa arid pipe d bate the method can be Fight Cc n athar bit bo menu ol operabont hat can be pertermesd in Hess mapatie kal ck lem am llu canne selected Flexchip Handbook 38 7 Available Buffers Blocking Buffer VP5 Deionized Water for Wash VP6 MN Method Run C FLEXCHIP 2 WMethods T raining Kit spr Method Setup XX Buffer Setup A Sample Setup AX Chip Setup X Run Available Buffers Current Buffer Assignment d Manual Buffer Name E Buffer Name Installe Prime 48 3 Running Buffer Deionized Water VP1 Running Buffer L Deionized Water for wash k Blocking Buffer 18 0 VP2 YES VP4 VP5 9100111027 wash Deionized Water for Wash A Each buffer used by the current method must be assigned to a valve position and primed before the method can be run Priming Valve Position 1 Right click in either list to see a menu of operations that can be performed in the respective list Double click on an available buffer to assign it to the currently selected valve position VP1 Prime MN Method Run C FLEXCHIP 2WethodsVIraining Kit spr d cot Method Setup Buffer Setup Sample Setup AX Chip Se
77. m Acquisition Dutput Format Comments Save ACD File Start Run 25 0 HE Prescan ROI Map Realtime Image Graph Scale Spocccsse2000000000000000000000020000000 0009090000000009990009009000900000000000900 990990099000 00000000000090909099009000000 09599090090090000090009000000090000000000000 90990009000000009000000909090900000000900000 99999090909099009990009000900000000000000000 990900090000000009000909209020900900009000000 90999009900990000000000000000000000000009 000900000000000900009000000900090009 0000000 999090999099000000000000000099 9099000909009 999909099099000000900900000000000099 0000009 CERO 0 0 0 0 0 0 00 0 00 CD 0 07 90990990000000000000000000009 000000000009 999909900090900990000900090000000000000000000 99990090900009000000000000099009909990099000909 99900990909090090900000000000000000000000000 99900099090 0000000000909990090999099 0009099 999009909900000009000090009000000000000909 90 6999999090999990999909909909999 000909990099 09 99909990900099090009000090090900090000090 9 00009 intensity R ls colored by ROI Type 19 degrees Start Run 40 Flexchip Handbook System Check Date Flexchip Control Software Version 2 00 0 0 3850001 7 10 24 2005 2
78. mage Graph Scale pecccsse200000000000000000000000000000 00090900000090009990090900090009009000000900000 990990099000 00000000000090909099009000000 09599090090090900000009002000009000090000000000 9 909900090000000000000090909900000000900000 9999909090900990099009000900000000000000000 99090000000000000009092090020090090000000000 90999009900990000000000000000000000000009 000900000000000900090000009000900090000000 99990909990990000000090000000099 90099000900909 9999009909900000090090000000000009 0000009 CE 0 0000090000000 0 0060000 CD 0 i 0 9099099000000000000000000000 000000000009 9999009900909000900009000900000000000000090000 9999009090000000000090000009900990990099000909 99900990090909000000 00909000000000000000 99900090900909000000000099009099009000990099 0009099 9990009909900000009000000090000000000009099 90 9999990909999909909090909099099990909 00990909 09 99909909090009090900090090909009090090909000090 9 00009 intensity R ls colored by ROI Type 19 degrees Result File Start Prescan 1 Method Run C FLEXCHIP 2 Methods System Check spr m Chpseup W Run Result File ROI Information Method Progress Selected Options Events Result File CNFLEXCHIP 24 check 20061024 acd m
79. ositions ValvePosiion 2 Valve Position 5 3X System Purged Valve Position 3 7 Valve Position 1 6 Valve Position 5 Nextll Please be sure the selected buffer lines are in the correct solution FF SET Pilr Z Progress Insert Chip Select Valve Positions 3X Prepare Valve Positions 3 System Purged i Cancel Next SET Pilr Z Progress System Purge complete Z Insert Chip Z Select Valve Positions Z Prepare Valve Positions Z System Purged 0 Cancel System Purge IDone 45 25 Flexchip Handbook 4 13 4 Chip Definition RE Pre spotted Affinity Chip COlumn 1 283 4 5 B 7 8 1 000 0000o 2 3 4 5 6 000000
80. p Time remaining X Chip has not been loaded Approve R lMatrix ROI Matrix Settings ROIMatrix has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute X Chip has not been filled Source Angle Image Llpdate 18 007 2 Continuous Apply Single Flexchip Handbook 7 45 Use Mouse to Zoom Image A amp 2 l Use Mouse to Select or Adjust ROIs ROI ROI RHOI 80 90 3 ROI l Fill ROIMatrix Inside Four Corners ROIs ROI NN Method Run C FELEXCHIP 2 Methods Training Kit spr JH J Method Setup W Buffer Setup gt Sample Setup X Chip Setup X Run Prepare Fluidics Status Fluidics Sustem is Ready for Run vf Fluidics Prep is complete Single Image Acquired Load and Prepare Chip o 9
81. positions valve Position 4 3 Prepare Valve Positions Valve Position 2 Valve Position 5 3 System Purged Valve Position 3 7 Cancel Next VP 1 VP6 Jata ES Progress Please be sure the selected buffer lines are in the correct solution Z Insert Chip Z Select Valve Positions Prepare Valve Positions 3 System Purged E Cancel Mili Q INextl Flexchip Handbook 10 2 79 Progress Sustem Purge complete Z Insert Chip Z Select Valve Positions Z Prepare Valve Positions System Purged Cancel System Purge 25 Done l 2 BIAdesorb Solution 1 System Purge Mili Q BIAdesorb Solution 1 System Purge 3 BIAdesorb Solution 2 System
82. pply Single Load a Stored ROIMatrix from a File gd C FLEXCIP 2 Methods SPR Performance Test Template rm 2 Use Mouse to Select or Adjust ROIs 7 OIMatrix is Ready l Uog Fill Options Select Fill Option Fill Chip Execute 1 F Bubble Blast Run Flexchip Handbook 96 11 FN Method Run C MFLEXCHIP 2WethodsYSystem Check spr F Method Setup Buffer Setup x Sample Setup l Chip Setup X Run EE BENIN EE K K K K K K K K K K K Result File ROI Information Method Progress Selected Options Events Result File c MFLEXCHIP 23D atassystem check 20051024 acd Cy Acquisition Dutput Format Comments Save ACD File m m Bi i Prescan gHummumumumumu ROI Map Realtime I
83. r Chip Definition Chip Definition Control 7 Method Editor Method Run 4 3 Method Method Editor fE Method Method Run Evaluation Evaluation 7 Configuration 7 Flexchip Flexchip Handbook 6 2 2 2 1 HBS EP 10X solution 100ml Milli Q 900ml 2 2 1 32 150M 10 2 5ml Mouse IgG 1mg ml 39ul 50nM 3 4 Mouse IgG 50nM 1 6ml Sample Tubes BR 1007 06
84. re the method can be run Priming Valve Position 1 E 2 minutes remaining Right click in either list to see a menu of operations that can be performed in the respective list Double click on an available buffer to assign it to the currently selected valve position Buffer Setup VP1 Deionized Water VP6 Deionized Water for Wash Prime 6 l Flexchip Handbook 11 93 Method Run C UFLEXCHIP 2 5 5 Check spr F MJ A Method Setup Buffer Setup Sample Setup X Chip Setup Run Available Samples Assigned Sample Name Vol mL io Test Solution 1 10 24 2005 SP1 BESSER OG 1 6 Test Solution 2 10 24 2006 Manis TM 10 24 2006 SP2 Test Solution 2 1 6 5nM Mouse IgG 10 18 2006 SPjRWae 1 10 Gly HCI 1 5 10 18 2006 Set 2 SP1 A SP2I c Set 3 EM sar Set 4 SP1 S2 Sample name assignments have been successfully completed Right click in either list to see a menu of operations that can be performed in the respective list Double click on an available sample to assign it to the currently selected sample position Sample Setup Set1 SP1 Test Solution 1 Set1 SP2 Test Solu
85. selected valve position 7 37 Available Buffers Running Buffer Assign to Valve Position 1 Current Buffer Assignment VP1 Running Buffer un raining Kil spr b gt no xc t Halhod5ahm Bulle Set X Sample Setup X ChpSenp X Fun Bulle Mana Manual Prime Dem Pass Die VOS esl ee A AE curent method maat be aviigned and prred blo rhe method can be xm Priming Vales Ponton 1 3 reig ars llusi s ls pm O poer cara fl r Double click on an bulfer In pir k In the Manual Prime Prime Prime 6 2 6 way 3 EI MWathod Run C WPLEXKCHIP 2WathodsAVIraining Kit spr Gg Q dh m j c ft s 5 X Bufe Seip 3 Sangia Seip XE IL Runa Asilsble Bulter Hame Descnimed WW aber yanin tar nter n wash Bul
86. ta raink Relevence Sensorgram 2 z Respense D Reference ROI ROI Excluded ROI Flexchip Handbook 8 59 8 2 k k Kp Chip Layout New Analysis Analysis Settings Injection Groups Set 1 Sample 1 v Protein 25 y Protein 50 v Protein amp G 100 Signal correction C Left column reference corrected data Reference corrected data C Non reference corrected data Select All Unselect 4 Help Cancel Injection Injection Set 1 Sample 1 Group Protein 25 50 100 as AG w LE NN N NCNCN RIN M N NON s Protein A G 25 wu 39 40 Calculsie Pendis Fer Groups Zambi Faye kay V kal Shena pounds mmm mm mmm mmm spot kd 1 s KD M Rmax RU Conc M rlomtuLiman kt RU Ms U Ch Ted T tmax Ska 1 Ms St kd 1 3 RU CES
87. tion 2 Set1 SP3 lil Method Run 2WethodsxTraining Kit spr F HX Method Setup I Buffer Setup v Sample Setup X Chip Setup X Run Prepare Fluidics Status Begin Prep Bypass Prep Fluidics System is Idle Intensity 1147 4 629 Load and Prepare Chip Clamp Time Complete Jog Bypass Jog X Chip has not been loaded atrix ROI Matris Settings X FOIMatrix has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute Chip has not been filled 4 d A 725 8 Source Angle Image Update 13 007 i Continuous Apply Single Load and Prepare Chip Dull Gold Affinity Chip Flexchip Handbook 94 F HM Prepare Fluidics X Fluidics Prep has not been run BEN Load and Prepare Chip Clamp Time Efe remaining Bypass Clamp X Clamp time has not finished mH Q E Q3 gom A i ROI Matrix Settings X ROIMatrix has not been created Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer X Chip has not been filled Source Angle Image Llpdate 19 006 Apply i Continuous Single v v Sample Setup X Chip Setup x Run m m s m s LE Preparing Fluidics m remainind Intensit
88. tup X Run Available Buffers Current Buffer Assignment Installed Manual Euler ane Vavel BuferNane Tr Running Buffer 48 3 Deionized Water 1 Prime Deionized Water for Wash Blocking Buffer VP2 VP3 VP4 VP5 Blocking Buffer wash Deionized Water for Wash Z Buffer assignments and installations have been successfully completed Right click in either list to see a menu of operations that can be performed in the respective list Double click on an available buffer to assign it to the currently selected valve position Prime Installed Flexchip Handbook 7 39 Method Prime Buffer Setup D Sample Setup FN Method Run C UFLEXCHIP 2WethodsVIraining Kit spr j 4 4 Available Samples Sample Name Test Solution 1 Test Solution 2 Water 9 Method Setup Buffer Setup Sample Setup X Chip Setup X Run Assigned Sample Name Set 1 Vol mL 5 1 W E Add New Sample s N Import Export Each sample position used by the current method must be assigned a sample name before the method can be run Right click in either list to see
89. tus Chip Flush Protocol 17 minutes remaining V Fluidics Images 0 80 min 3 384 frames sec Load and Prepare Chip p Jog Chip is ready ROI Matrix Settings R lMatrix is Ready ROlMatrix has been approved Select Fill Operation Chip Flush Select Bubble Blast Buffer X Chip has not mmm Source Angle m Image Update 18 007 Continuous WI Ei Apply o Image Updates Continuous5 Chip Flush 9 Do you want to wash the Chip Flush lines at the end of this run wash is recommended if no run will immediately Follow this run Chip Flush es It s Time to Move the Buffer Line Please move the buffer line valve position 5 to a wash solution Press OK when the line has been moved Press Cancel to not Flush the line at the end of the run Chip Flush 50ml IOKI A Chip has not been filled Chip has been filled Chip Setup
90. uidics Prep is complete Single Image Acquired Load and Prepare Chip Clamp Time Complete Jog Bypass Jog X Chip has not been loaded o o Approve R IMatri ROI Matrix Settings X ROIMatrix has not been created 07090 Fill Options Select Fill Operation Bypass Select Bubble Blast Buffer Execute Chip has not been filled o 0960 0919 70TH 5 9 0 0 o G Ous X ON o E o OO Qr e Ky OON LO 0505 0 6 6 000000 o o o e 060800 600006 9 9 e 9 6 Oo OR e o e 6 6 8 6 6 Source Angle Image Update rd e 13 007 I Continuous Apply Single Uog has not been loaded Chip is ready Flexchip Handbook 7 4 Jodg ROI l Approve ROIMatrix ROIMatrix is Ready ROIMatrix has not been created ROIMatrix has been approved Wil Method
91. ump Offset APM Flexchip Chip Drawer Control Select Calibration Type Recalibrate Old Tubingl Caliblate New Tubing tGuliuratiut Chip Drawer Control Progress ms Z Chip in place Save Z Calibration Type Selected 3 Sample Line Primed 3 Calibration Point 1 Measured 3 Calibration Point 2 Measured Factor Calculated Cancel Ready for calibration Select Calibration Type ES l E Prime Sample Line r Prime Line Now Already Primed K K s Calibration Point 1 d Sat Pump Calibration Point 2 9 EM Sample Pump Factor tpm ul min 0 11 Pump Offset APM 0 Flexchip Handbook 10 87 Prime Buffer Line Prime Line Now 5 7U u 52 5 Please place a sample tube filled with at least 15 ml of fluid in sample position 52 and a collection tube under calibration port Press OK when the sample tube holder is in place 1 6ml Milli Q 82 Flexchip Flexchip Accessories K
92. up Sample Setup Chip Setup X Run Method Name System Check Comments This method is used to verify operation of the instrument lt should Analysis Temperature run at 25 with a Gold Affinity Chip and with deionized water as 4 Method Value Apply Now running buffer Samples Current Setpoint 25 0 Current Temp 25 0 v Pre run Options wash Prime Load Options Cycles Pause min r r Cycles Pause min poen s Je s Flow Rate Duration yuL min min 500 00 20 00 500 00 3 00 500 00 5 00 500 00 3 00 500 00 5 00 500 00 3 00 500 00 3 00 Operation Buffer Selection Buffer Deionized Water Set 1 Sample 1 Buffer Deionized Water Set 1 Sample 2 Buffer Deionized Water Set 1 Sample 3 Buffer Deionized Water e m k w N Apply Now amp 77 7 2 L C Current Setpoint 25C l Method Run C FLEXCHIP 2 Methods System Check spr amp Method Setup XX Buffer Setup XC Sample Setup Chip Setup X Run Available Buffers Current Buffer Assignment Buffer Name Valve Eur Nam Installed Manual Running Buffer 10 11 2006 Pos Prime Deionized Wale ve Deionized Water for wash 10 11 2006 Blocking Buffer 10 11 2006 VP2 VP4 VP5 wash Deionized Water for wash Each buffer used by the current method must be assigned to a valve position and primed befo
93. y 913 1 3076 Rl L EEHEHEEREERNHR Prepare Fluidics Begin Prep Prep 15 Approve ROIMatrix ROIMatrix Setting DKI ROIMatrix Settings Settings for Fil ROIM atrix Columns Rows Create Reference HHls Reference ADI Size Factor Reference ROI Pattern Left Reference ROI Position Factor ROI Finding Approx Diameter pixels Cancel 09 BEEN 15 Spot Appearance Dark Spot on Light Background Columns 20 Rows 20 Reference ROI Size Factor 0 9 Reference ROI Pattern Left Approx ROI Diameter pixels 15 opot Appearance Dark Spot on Light Background Flexchip Handbook 11 95 Method Run C FLEXCHIP 2WethodsWSystem Check spr F HM Method Setup Buffer Setup Sample Setup Z Chip Setup X Run Prepare Fluidics Begin Prep Fluidics System is Ready for Run vf Fluidics Prep is complete Inkensit 1016 302 1107 Approve ROIM atris ROI Matrix Settings i atrix is Ready Fill Options Select Fill Operation Fill Chip Select Bubble Blast Buffer X Chip has not been filled Source Angle Image Llpdate 18 006 zi Continuous A
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