取扱説明書 - 検査・診断試薬（非医薬品）
Contents
1. Bx MSDS2. LAMP Loop mediated Isothermal Amplification LAMP DNA DNA LAMP Eiken GENOME SITE http Iloopamp eiken cojp amp amp
3. 1 lt 3 LAMP DNA Bx
4. DNA 2 0 ul Bx 1 1 UV 1 x 3 63C 60
5. 20 C 6 20 C Bx Bx DNA DNA
6. 10 18 0 ul DNA DNA 2 0 ul 20 0 ul
7. 60 80 C 2 UV UV 240 260 nm 350 370 nm 320 nm 60
8. 90 80 C 2 UV ZN Bx Bx Bx DNA DNA Bx
9. 1 2 UV UV DNA DNA UV DNA DNA gt
10. Bx Bx LAMP Bx
11. DNA Bx 15 mm DNA Bx
12. 1 MSDS 3 1 5 ml 2 0 ml 15 mm 55 C 94 C 63 C 80 C 0 5 10 ul 10 100 ul 200 1 000 pl 02 ml
13. ISO 13485 2003 http www nippongenematerial com 2 24 NE0041 96 NE0043 2 Se 1 1 24 24 x4 24 Bx 20C 20C Bx 10mlx2 10mlx8 20C 24 24 24
14. DNA Pine wilt disease
15. Bx Bx B 1 C EDTA D
16. http nippongene analysis com LAMP LAMP LAMP LAMP e Genome Order
17. DNA a a
18. Bx 20 C 1 x 3 1 5 ml 2 0 ml 1 x 3 lt 5ri Bx 16 4 ul 147 6 ul 1 ii 1 Bx
19. A A A 240 260 nm 350 370 nm UV 320 nm A
20. UV 240 260 nm 350 370 nm 1 5 ml NE0051 4 7 3 1 15 mm 2 Bx 800 uu 2 wr Bx 3 55C 20 DNA 4 94 100 C
21. DNA LAMP DNA Bx DNA Bx Bx 63 C 60 LAMP DNA DNA DNA DNA
22. Bursaphe lenchus xylophilIus Detection Kit VersIon 4 0 0 NEO0041 NE0043 DD version 4 0 0 1 LAMP 2 6
23. 1 1 gt iD 100000 ppm 1 ii 70 iv 1 v
24. 2 0 ul 1x 10 16 DD 2012 11 NIPPON GENE TEL 076 451 6548 TEL 03 5977 5573 FAX 076 451 6547 FAX 03 597 5574 E mail suppot nippongene analysis com E mail feast egenome csJp fujitsu com URL http nippongene analysis com URL http genome e mDpJp Copyright 2012 NIPPON GENE CO LTD All Rights Reserved
25. e Genome Order http genome e mpJjp Si Se Gi 1 LAMP Loop mediated Isothermal Amplification 2 NS i 2 3 a ea a aad aaa a ea Vea a ena 3 4 SR RR RNR RR NN 5 5 O he 7 4 HEC 9 DNA 5 RCS RN 14 6 NN ON 15 7 NN A 16 Bx 1 LAMP Bursaphelenchus xylophilus LAMP
26. B 14 6 1 2000 88 5 407 2 Aikawa T Kikuchi T Kosaka H 2003 Demonstration of interbreeding between virulent and avirulent populations of Bursaphelenchus xylophilus Nematoda Aphelenchoididae by PCR RFLP method App Entomol Zool 38 4 565 3 Kikuchi T Aikawa T Oeda Y Karim N Kanzaki N 2009 A rapid and precise diagnostic method for detecting the Pinewood nematode Bursaphelenchus xylophilus by loop mediated isothermal amplification Phytopahology 99 12 1365 4 Notomi T Okayama H Masubuchi_H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA uc e c Acids Res 28 12 e63 5 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 15 7 Bx 2 0 ul DNA 20 0 ul 1 DNA 63 C 60 Bx Bx
27. 10 800 ul 2 Bx 55 C 20 94 100C 10 DNA DNA Bx 1 800 pl 20 C 3 Bx Bx
28. 10 5 08ml 72l 200m ezou 45om Bx 1 5 1 18 0 pl 6 4 DNA 2 0 ul Mm Bx a DNA Bx ne mm mi mm mmm mmm mmm mm mmm mmm mmm mmm me FR mae gente lt No 20 0 ul 63 C 60 60
29. DNA DNA 13 5 A Bx
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