Biacore T200 取扱説明書 応用編
Contents
1. Toas v Jo Adjustment for controls nlnr By k Sort d IF Fitting k Adjustment Far controls Ranking Table Columns Biacore T200 1 15 urtsw n lar cns rais ER berg raa rure IT aa ic TM Lug Line heerd ky conici Poser cmi CELA leyre di E Feng iei ni Befone adjustment Adjustment settings Use adjustment for controls Positive control Negative control2. 1 4 1 1 2 Anolysis_temperature Sample compertment temperature 10C Double Mix Analysis temperature 25 CSample compert
3. Adjustment for Controls Biacore T200 142 6 Hmmm qo artum mum bagmmerrpa Fewamom iacens Em Com ma er p a i ep eea ms el EFgua ma Lad EE Biet Fie Fen After aiuanment Eeg F rimerr earum einn eund f Ld Fome Feer mier rri mn a um Li Adjustment settings Use adjustment for controls Positive control Negative control Fitting function Linear Polynom
4. OFF TEL 03 5331 9336 AL ERIT 9 00 17 30 0 TEL 03 5331 931
5. Mie iga nix F Remove Selection 7 2 Undo Biacore T200 3 91 xil Tiie rin mi d lr e 2 lzm En a a le d EIL pom s iati y EPCWH i mem jan vv um a p e us m i di I ij g ARN 323 p iex 39 gu Wer j T e ETIE LE ren i in mm ME X IUNMD I MC E 03 NEED m E ru L RENT UG U Sg F Lair jonm iren ceai ran LL d inf D Eee ad ccs Curve mprajo l 2 i X sd m pum na Fm piii eaan oe morie laa Cen 1600 Fre bougie A pD mouse ga i140 mouseigG xT1400
6. 20 isopropanol 40 mM NaOH Extra wash ADAs 2 ADAs 2 human IgG lambda human IgG kappa Desorb gt 1 Desorb Cut off 48008158
7. Menu Export Positions a a Menu Sample Position Import Biacore T200 3 51 3 3 Control Sample R1A1 H5 R1A12 12 m n j Z T i p z d i i NH n a EIT 1 L E P I v E E i SC E W Ooooooo0O0o0 QO E
8. AbsResp Baseline 50 RelResp Baseline RelResp Binding level Evaluation Explorer Biacore T200 12 1 Binding level 2 Evaluation Explorer Plot Binding level B Hindis i Ending level Fare ems 120 T g eU amp 20UvZL BHBUIU
9. A 2 B DAH AS Z gEAUT10 c f05clXe amp zaocueccDBDDUzxd d ddbs5 BRW 2 1 i 13C 21C c 29 C o ii 37 C c O i Q pl ec tc qtd kJ mol kJ mol A Bw AB AB 217J32BJ V2 5 9 ORERNTSEHIB 4 RIEME l2 d5UV Cla 2 353072 74 2 ARREMTASEBEL BEREEE Kp ka k
10. binding stability co binding stability co_binding co stability 1 Carry over control Regeneration Biacore T200 2 33 2 8 Wizard Binding analysis Kinetics Affinity
11. Menu Export Positions Menu gt Sample Position Import Biacore T200 170 6 LS TII Ier nr XM ns Sanguinis hona si xIM f U Prepare Run Protocol Mika done The conti Tenim thp i d cked Maka nao 3 ramp es A resgerir 403 loaded ihe ryck and meropisee accaondng 0 f Hek Meien amp etum Vus ihinda ba prala wih ruth capa and re nm edt adherires frsl Fior lAo Buffer on he lef hand Prag and imer Th Fmrrmrl boiagi r below Hobe SIEHE wer run will une bular dh Make sure him i merh waer in The water Bottles an Tha ngil Farid ray He stu Ihe hun Pa run ume oh cS man EL Corina aerei lempus nca chen nd direta osi E rue nam cro mgr t a Aurr tfm 1 TIE plur Em Ks wawado uus ran ge we EURBVGIERSSBIR WERA 52281352 7 7 R SLAREST SGnctTisd Stat
12. Menu Export Positions Menu gt Sample Position Import Biacore T200 6 149 E Iramunmagrinicity Cuonfrrmatiun Prepare Eun Proiocal Prepare Run Protocol biako zum the cnirpci sensor chip is docked Make zum af camas magenlz are laaced in the rack and micraplate according Ln he Rack Fcrstinns zetup vals should be saalad ilh ribber caps and micronlata wnh adhaziwe Tol Place ike bulene on the left hared iray and insan ihe correct Tune ee bere Hotel Standby afer run will usg bufar A laYE sure there is fresh swale in ihe waler boile an ihe ighi hard iras H nacessaryg eempby tha wagle holtle hefnrn start cf tha min ElmrrsisHd rum ree 12h mm jexcluding condeonal ena Femperssum chesnges end stsndbg Bos E lim md bli co en 3 1 Flunrang buller art EE md plus 65 mias hr mirHL abe nn
13. Calibration Curve EIE ml SU x20Uv2L Bio9 Curve 1 kal Calibration Curve EN Ctrl Cure epi Ce we Cak Cam ER ome Deom ma mgr iy drug en m a Biotin I Li 1390 3 Ted i Use calibration trends Calibration Table Conc Cqlc Conc CV
14. E zm Zee amp i d a 0 5 Binding to reference Stability baseline 23x d 4853480 70 v F 75 NN Biacore T200 3 3 1 Biacore A B B Z A b RU A
15. Kp ko k Biacore T200 94 4 vant Hoff Ko AG RT InKo 4 amp A iimtercept on Y axis AG AH TAS Jar Inko AH RT AS R AG kJ mol R SEE T Ps T K VH R slope Kp M AH kJ mol AS J K mol T RTInKn AHT TAS ACP T T5 TACalnl AC KJ K mol To 25C Eyring Ko ka k CkeT h exp AS R AH RT I
16. Save as Methods and Templates 7 Bia Users Don t Save Save in File name Save Standby flow Biqcore T200 Evaluation Software HB Biacore T200 6 171 6 4 2 lt Evaludtion E 6 13
17. mol ms x km N D xf m 0 98 x 0 3 x h xw x D f h w Biacore T200 FE 3 12 CFCA 20C 324 3x10 11 fxmreixMu173 m s f FRR T rel 20C Mw s Da
18. AbsResp Baseline RelResp Baseline RelResp Binding level Evaluation Explorer 6 141 Toolbar sz Immunogenicity 2c 7 J 7 xj Sceening l Settings Curve Fc 3 Report Point 2 enhance_level 2 stability Regions amp Boundaries Number of Regions Boundary Region Name Finish tE 6 5
19. 0 5 TFA 0 1 ZipTip Milipore 50 mM NoOH NaCl Surfactant P20 DMSO Biacore T200 112 5 5 1 Toolbar Run Method Menu bar Run Method
20. GE amp um T 169 0073 eme nm 3 25 1 Intertek TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com
21. n i Biatin Hi 4 i 1 1 AX V rh ase ree E grace T oye aane odi ecu lat E riam E vum aar IE Eben Lr ui DOD 34 ics 1 Topy raraph n ae Cm Curve mi Ca canum d wom i OAA a C Arye PER abun nad Exclude Curve Calibration Table Cah Cmm m Dy a drea nd inu g itu P Biaotin im mi r 105a Le a TiDi a 1 E a L D dv 1 n Lad ims t 1 TEE 4 rm r 1 k BU E 12688 3 1 5 i Mrs TE 1 50 Tr LE a ES TE E r 158 7 L WA g TOL Bod LE TI tI mm E aj m TEE I ime riran gi Include Curve amp EIRUS S Biacore T200 3 61 E 3 9
22. Menu Export Positions isi Menu gt Sample Position Import c g7wVvytueBDzesnssg Biacore T200 6 135 LS TII ET la Xu nns Sang hona si xIM f U Prepare Run Protocol Mika done The conti Tenim thp i d cked Maka nao 3 ramp es A resgerir 403 loaded ihe ryck and meropisee accaondng 0 f Hek Meien amp etum Vus ihinda ba prala wih ruth capa and re nm edt adherires frsl Fior lAo Buffer on he lef hand Prag and imer Th Fmrrmrl boiagi r below Hobe SIEHE wer run will une bular dh Make sure him i merh waer in The water Bottles an Tha ngil Farid ray He stu Ihe hun Pa run ume oh cS man EL Corina aerei lempus nca chen nd direta osi E rue nam cro mgr t a Aurr tfm 1 TIE plur Em Ks wawado uus ran ge we EURBVGIERSSBIR WERA 52281352 7 7 R SLAREST SGnctTisd Stat
23. Save as Methods and Templates 7 Bia Users Don t Save Biacore T200 1 9 Save in File name Save l REIA Xu Standby flow l Biacore T200 Evaluation Software 73 E 1 5 Run Stop Run Hapcure TAOL stop Run Fin Sopped brecha map e please val Pw mn Ing E hf iEn
24. Biacore T200 82 3 Eject Rack amp 2 U v 2U C Rack tray port l OK Eject Rack Tray Rack Positions Next gt LE FEW a a D E E GgLIUREG a L pe ll p start Save as Methods and Templates 7 Bia Users Don t Save E F i um r1 EF s a m gii Ea il P Save in File name Save Biacore T200 3 83 A
25. Wizard Biacore T200 Biacore T200 34 2 2 2 Evaludtion Biacore T200 hi IN d j DU
26. Control Sample R1A1 H5 R1A12 12 m n j z T i p z d i i NH n a GiT 1 L E P I v E E i S E W Ooooooo00O00Q a E SHPPRGUE M hm Te LLLI ILE ne a Elk LMLELLCCLLLISUPEIT Lr LII d E her dem hem mask SUUS UE HD DET Bnr p 009 3 Eua Pi FI el QU GUY s himt chupa s m E l dg e Ru we pM PITE E FE m ng gt domum s gt Bap 5 cg iiim d o CE ial ahe mermi i e hr L imr r Baia i 5 fum om i E i iu i i oo Auto Poolingo_ Yes OK Automatic Positioning
27. Keyword Table CELU g Tools Keyword Table 1 i alla amp E scn m ME EEEE AIDI i fnnam W y mHli w ENBMz m Eg T HE HHHHHIHIHHIIIHI apuctpwEESmwg Biacore T200 6 151 Menubar Toolbar m E LB deu Sensorgram window Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer 0 llot Baseline AbsResp Binding level Baseline 73 50 RelResp Binding stability Baseline RelResp Binding to reference Binding level Work area Evaluation Explorer Biacore T200 152 6 Toolbar zz Immunogenicity z ER 2 Confirmation lcd E Aa am e Tios
28. i cran omn en uim aan Dade Pun Lim Prime Normalize Temperature settings Analysis temperature 25 C Sample compartment temperature 25 Cycle Run List Lyi Amap Erp s uarie Wim ia Bpod ampie D T Da EFi 2 2 tup a Teng halte i E Aral k 1 EE im J Tangis TIE a ege ET ILE Een Hi Eua en li 11 mu CIL iF ag m E3 de pu yg JF andareede Ix eu rd Eb wg dm sal abad Biacore T200 28 2 Next gt p MH W 1 Lei adl ansias L nd Een aper L43 adl ardiyracie x X Lag Weera E L83 Ag mmu L3 Aw degun M Lan Wei adus a8 Aisi ETE m La3 Weira mm T s La3 Wri rE A A LA d LJ JFT hia Waita iaa p n pem P4 M M OO X j F3 LEi aE oc A i OO OO L9 Wusfaine LE E uu Lad Dadfes sd budfus DAL alf ETE SrL DEO E ru Tjj Arm De m mis Fal oben coneneonl RSS amen bes Aj Ful FT mist e bw Al Ful Tohari comer biwi paraire A Pul Baiat r
29. No n Biacore T200 76 3 c aum porum c uar m ras UN umm Samir er Tere camions r i LU E begi o Perinde i3 Assay Steps Blank Define some values in Method and others at run time Flow rate umin Setup Run Sample solution Buffer MW Da D 20 C Blank Yes _ Dilution Verification Biacore T200 3 77 The Method has been verified and can be used to set up a run a Setup Run Flow path Next gt
30. View 44 Samek mouse Ig 31 6400 Caec Fees 7 JH Shaw Blank subtrached data Show blank subtracted data Advanced settings gt LTEM LLL TELE Apply settings to DO Single Sample series m Biacore T200 90 3 YY 3 3 3 19 Close gt Next gt 3 19
31. Y 0 RU Fitting function Linear Polynomial Y 100 Y 0 OK Biacore T200 16 1 Evaluation Add Report Point Table Evaluation Explorer Report Point Table Work area ei i p Tina Lake l em We mmm Temp nami newsm HH hm Fu Feme me s Hd E Ej E E 22 Li E ry k E L Fus Vas rA X Emi 3 15
32. Exclude point Exclude curve Show Sensorgrams 5ralg Copy Graph Export Curves Exclude point 2 Y w2Uz 3 1 i 1 Biacore T200 38 2 FAE 2 12 Solvent correction Included dnduded yde a Curve Chi RU Ri w A4 ia n 2298 LE y 2e 4 3 057 ual 30 Fcmd 3 T rrET 1 n Biacore T200 2 39 2 13 DMSO DMSO
33. Poolingo Yes OK Automatic Positioning Eject Rack amp 2 U v 2U C Rack tray port OK Eject Rack Tray Rack Positions Next Wybe LL T8 COUTEL Rej 1 Fej rni Od Nin Make turg Mb oampees EL reagent ve aded the rack aed mesopas acordes ico jhe Fark Paa erm tu rm hon ba 14 ale ath ribbar capu ars rrezroglaig h ajdhazem heel Ie Pues iba bolerjds mm dion uL hired rap and mian Te TFT Tuin i eee biben IX Sb Hi rum cw urs bue A Mala sure Tep HERE man e j EET ome on ihe nfi hand P334 TY Wh mia bois bails ET o LE E imisi gan mn TE men Eg Ce EL a LE TE deni Dundee Noi ee FF EE Ai be TC d plus 85 mlda dor nlet siie rum H Be stat
34. Double Mix Drug ADAs ADAs Double Mix ESSE dus Biacore T200 Sensor Chip CM5 Merged Inject Fc3 4 Fc4 ADAs Fc3 150 kDa
35. Biacore T200 30 2 Eject Rack Rock tray port OK Eject Rack Tray Rack Positions Next gt LESEN Dep rne deckad nh rure Mogampses E regen sre ajed in ihr rack aed mipik acconkng ip jhe Bark Prina rm nh be pied with nbhar caps and macroglais adhazmw hel Puig iha bulleri im fion E hired rap iid nobel Te corner Tuban iine bibi hi Span afar run edd usa bui A rm hand Eas E Frerestdarg memphytWhewb bote babe gian pies ng rhmaa mi mm Th Dew jha rzwickuowek r nbehenrl deep nee Duel Bud phaneibs Bossi BARNERA HERSH 5 EBGLLIBITZ ESD amp SAZmneixS stat Save as Methods and Templates 7 Bia Users Don t Save E e r cara
36. ADAs ADAs 6 2 1 Toolbar Run Wizard Menu bar Run gt Wizard Immunogenicity Immunogenicity Confirmation New ENESE E D 1 Enhancement Regeneration Next gt Biacore T200 6 145 E benwnesenlchy Conflmatkon Setup London am Contei ime ll Humber o necne mW Bun raum epele ehh LIFE CT Hu ri aes 3 LE 3 Next gt l E Wemumgenlrr Confirmation I
37. Eme c ESI ESTIS Assay Steps Biacore T200 70 3 CD Startup Number of replicates times 3 l Biacore T200 Sample Number of replicates times Biacore T200 72 3 Blank Recurrence Distribute occurrences evenly 1 Number of replicates times Cycle Types Biacore T200 3 73 Startup 2 Command
38. Open Browse Open LES Lome raion Amia In jer lin tenquanacg SAREI C ht rerom 2 em 1 Biacore T200 Detection Flow path Chip Chip type Capture Sample Enhancement 2 Regeneration 1 or 2 Next gt Ta Comme ration amp nabis estu mi Wumb congr am Ee rli gp sn ms
39. Walue VWalue amp Y Value amp Y Value d Biacore T200 1 13 Color By k Sort k Curve Fitting FI Adjustment For controls Ranking Table Columns Us Ranking Ranking Ranking Settings Mone CO One Ranking Boundary 6 Iwo Ranking Boundaries Ranking Boundaries First Salue Second Value 59 Help Finish Cancel RU RU One Ranking Boundary 1 First vqlue 2 RU Two Ranking Boundaries
40. Open Browse Open l S immungenieliy Screening Run Typa To debet anie Euu to drug ri rer zampe samples mae De acria bo imimaze de arhbode ad den rme mmedately rr meapaemeni How do you vani bo peso the measurement 2 Dred analis delecis only lem antboden J mbinn aine nesurraliz ann doci amnibocies in Ihe presence of exce dna Ce Le ws Acidification and neutralization Next gt l mW Biacore T200 6 131 T kmen EE Sere or ET LTT LE EDAM RANDY 1 WEDESERATIDN F 1 Enhancement Regeneration Next gt ES immunogenicity Screening Setup Conditioning Bun conditioning cycle Solution Contact time s Mumber af injections Startup Run startup cycles Solution Startup pas control Mumber of cycles
41. Biacore T200 2 25 Number of cycles 3 Solvent correction Number of injections Repeat after _ Sample cycles Next gt lm ka init PT IIa Lm Dr ie 1 b mem EE dimer Ci ima sta mar atem Fe P Des Mabik m pei i Bak jew Sample contact time 120s Flow rate 30 ul min Dissociation time FERE ST fes 120s Extra wash after injection with Stabilization period Os Next gt Biacore T200 26 2
42. D MES Web Biacore T200 D Web Biacore T200 http www biacore com diffusion_calculator Diffusion Coefficient Calculator Converter UserID Password LOGIN GB Biacore T200 66 3 Diffusion Coefficient Calculator Converter ss ca p c E mn 5 nc CHETI Ent LET Molecular weight T Erbe 2S FUTTER 2 i I a il Tem i i Bailar F VELEZ amp IT Fs 3 ia aaar a gri i i i I mml u B N ri Eua _ HETT diffusmn cosfheient iram temamnermture IFL lampo mme 5 D mi amena T 20C Molecular weight 73 Da Friction ratio FRR OChoose molecular shope Globular 1 2 m 3
43. 2 5 100 ul min km D BDNE 2 11 Kinetics Mw km Bivqlent Analyte CFCA _ Christensen Anal Biochemistry 1997 249 p 153 Sigmundsson K et Al Biochemistry 2002 41 p 8263 Biacore T200 64 3 CFCA 7388 Z 5 000 Do D ko 10 gt ka gt 5X10 M ts t 4338 150kDa 5 000 RU
44. o A252 0 53 0 60 1739 4 94 0 08 o 401086 7 238 03 19827 Biacore T200 6 125 6 Sj E Eau AHBDEBSS9TIV28Ie5 amp nwec RRES EZV EEan He Anti drug Antibodies ADAs Biacore T200 ADAs Drug ADAs Merged Inject Double Mix ADAs ADAs ADAs
45. 3 Number of injections Temperatures Analysis temperatures Repeat after Sample cycles 25C 5 Sample compartment temperatures Next gt Leis ia 1 b ml i mm Ci Vea a ar atum Fh culi hacerse aus jo Aaa HL mE T blah pex rp PF TE Pr Hmmmi H bd fheim f Wiii abeam pend ll pip IL Sample contact time Flow rate Dissociation time 1205s 30 ul min 120s Extra wash after injection with Regeneration Solution PP Biacore T200 98 4 High viscosit
46. Fc Report Point binding Assay Step Sample elResp IRI 2 Biacore T200 1 17 1 9 Evaluation Explorer Plot controls binding I Larntras birding pam Lack m reru Fade a rce Evaluation Explorer Plot Binding to reference Dji Binding b reference D Zur bx Biacore T200 18 1 1 10 Tools Custom Report Points
47. Biacore T200 68 3 3 2 1 Toolbar Run Method Menu bar Run Method Calibration Free Concentration Open Method Builder Main Overview Biacore T200 General Settings Biacore T200 3 69 D Data Collection rate 10 Hz Detection 2 Dual Multi Dual 2 1 4 3 Multi 2 1 4 3 2 1 3 1 4 1 Sample compartment temperature 4 45 C 25C 4 Concentration unit Buffer settings After run
48. Save As Methods and Templates 7 Bia Users Don t Save Save in File name Save Standby flow Biqcore T200 Evaluation Software HB Biacore T200 136 6 6 2 Run Stop Run Himmi TZ stop Run Fireshareg CUETW cycle pieg vil Ps Fn Ing E hf Een Ctrl Breokl
49. Sample id MW Do Concentration nM ug ml Control Sqmples e Dar rr angie DU Tage CORSO Leggi ic dert Lud parque Labia pde ki T CU APT a E Biacore T200 2 27 Control Sample definition Run control Samples 2 Repeat control Sample s every Control Samples Next gt 2 3 Excel Excel Excel tt
50. DMSO 10 0 05 Surfactant P20 is 2 1 Mat ARSED PBS 10X 1x1000 ml BR 1006 72 pH 0 1M phosphate Buffer 27 mM KCI 1 37 M NaCl gt 10 SARA DMSO 0 01 M phosphate Buffer 2 7 mM KCI 0 137 M NaCl 596 DMSO pH7 4 2 27 IF sS LC 18eUM DMSO DMSO 0 22 um DMSO UV spectrometry DMSO pH DMSO pH
51. Biacore T200 Evaluation Software DMSO DMSO 1 x y NO N NO D 2 DMSO 596 DMSO DMSO DMSO 1x PBS no DMSO 4 6 DMSO 4 DMSO
52. Add krd 11 Fe part mim ELLE s hl soon fra mar FE umchrvd aem airulats ere FT Iz procos parT c pp la cd Aina iep pupocs prm T igh la i i Id Cycles OK f Custom Raport Pninis Custom Nepot Ponti aA Id T1 Position Assay step purpose stabit y J eco aftwr eon ol C sir abc rig tion a 1 Eei Fa 31 117 1 a mag Y ib Biacore T200 1 19 Bar Chart NN epe HEHH TT TUER REDITU HO Li caa a Curve Tools Tools WF FnUD Bv k Color By k Shaw Column Labels Biacore T200 20 2
53. s Next gt EE o ou Sample id Control Sqmples Biacore T200 1 5 Fm Phan conso ampie 7 Rapsa cords piaj gens Repeat control sample s every OK Next gt 1 2 Excel Excel Excel txt BEE EE LE TI EE bap Prime before run Normalize detector Analysis temperature DNE Sample compartment temperature 25 C B
54. Biacore T200 160 6 6 3 2 Double Mix 6 3 1 Immunogenicity Isotyping Toolbar Run Method Menu bar Run gt Method d ar j E 1j Eli Open l Method Builder D Main Overview Biacore T200 Biacore T200 6 Sor ERAS 161 Sample Settings for Sample 1 Type Double mix L Acid solution Neutralization soln Double mix 128 2 Enh
55. On o m EILAN ORAAL QU 5 35 91 5 3U 977 0u 3 JALAN OHE HITA Ab IC 60 Qe a5 B S B 8Bila 2 FRP CRUS VN i EEBOIBNICIAU x o o
56. RU vs C u l A A Slope A Slope vs C 8B A A 1 1 A A A s A A B ga IEECI UL IEEEOG AR UNDUU RRIDO B A 1 Hiem RU B60
57. 2 _ DMSO nM uM Kp 1 10 10 5 DY YTRA PBS HEPES HEPES DMSO DMSO 5 DMSO
58. Wamo l or sl alle Eonmperaburr Tn abbam nelle results vor qdhouki war Ti ce tempu itunes gu qtiae eet ET atum erre breeperabuer Ii Temqer aure st aobie red abe ren l HERE e Eld Standby flow l Biacore T200 Evaluation Software 73 E E 4 4 ZD072 403553 Run Stop Run amp 2 Uv 2 Us Hiromi Perd TN stop Run Pb cyche Ing E hilfe Ctrl Breakl Biacore T200 Evaluation Software Biacore T200 4 103 4 2
59. ADAs Merged Inject Drug ADAs ADAs 1 2 3 ADAs Merged Inject p KU IR Merged inject Bret 2529 fe IET OD m ptT SES SETAE mundi x E neg Wu LII Bec 1 Merged Inject Biacore T200 126 6 BATEC e FEL i J F f pi T T I Pia xe sm rituximab lug mll xntuximab ug mll TI ma terum am egeo mon 2 ADAs 3 9 7 Z U c ODSETIREE Z2 d5 7 ADAs
60. Ctrl Breakl Biacore T200 Evaluation Software Biacore T200 10 1 1 2 Evaluaqation E 1 6 Keyword table CAEL amp g Tools Keyword Table ET 771 74 3407 85i iti W 5 RMrmES T iul HB H6 E HI mg H E N 1 EHE ASERSRSEBEERE MI IEIIiIII az EAEE i Biacore T200 Menubar Toolbar Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer W nsorgram amp pio Baseline Binding level Binding stability Binding to reference Work area 1 11
61. Keyword table CAEL sg Tools Keyword Table INL e LE e m 4 ar yr rar m aE mm E EE EL E groom Hmm mm m siliga a mmmMmusSM zu du NB g EE ETEA F m Eg f i j i HE TI HE Biacore T200 172 6 Menubar Toolbar Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer illl ouram amp oio Baseline Binding level Binding stability Binding to reference Work area Biacore T200 m E LB deu Sensorgram window AbsResp Baseline RelResp Baseline RelResp Binding level Evaluation Explorer 6 173 Toolbar 53 Immunogenicity lt stabiity n IEEE lui iem erT ira
62. Biacore T200 Evaluation Software Biacore T200 6 137 6 1 2 Double Mix 6 1 1 IZ L Immunogenicity Screening Toolbar Run Method Menu bar Run Method d Open Method Builder D Main Overview Biacore T200 Eli R Luiz FGustuluE uc ELJ HNTGUENNNM Maa Biacore T200 138 6 Cycle Types Pe her LE dum B Fs 71 Lge pg jue LE Sample Setting for sample 1 Type Double mix Acid solution Neutralization so
63. Control Sample R1A1 R1A12 12 F J E i or m er in T LII gr JE TE j H i T ET n m 1 L r 1 i E y E i Ooooooo0O0o0 QO 900000000000 x r mue Deere eom D Haec en n pero D an imd ei Rd Tig ia ps h EE bi n fir Nfl LII d E her dem hem mask Wis t Fg hl uam hiep lap ed Ead Pi p j LT obe x eui LI ut BS 2 BAT m a puM imd 4 E v E E LEE E mM FEE e LEF E a lm rmrmimm aie d m r Celer T da um i 1 iamm booling Auto Poolingo_ Yes OK Automatic Positioning
64. Repeat control Sample s every 20 Control Samples Control Sample id Expected conc Next gt LEE EI OIM M Biacore T200 3 49 Sample id Dilution factor Next gt 3 1 Excel Excel Excel txt LES Piarik a Altini y kys Har parai vp Pres is Fm 1 Hepa gea epe unm cu Prime Normalize Analysis temperature 25 C Sample compartment temperature 25 C Cycle Run List
65. CFCA 0 05 5ug m Cd 1 ug ml EZ Z3 Cd RHE 280 nm 10 4 lt 5 ul min 100 ul min CFCA IgG CFCA gc IgM CFCA CFCA 3 11
66. DMSO Solvent correction Extrapolate F3 Solvent Correction Extrapolation Range Estrannlahinn Range x asts 1000 RUJ ERS DIBEADLEJ 10 OK i EN Biacore T200 40 2 1 73 3 EBfRI1E 100 Evaluation Add Plot Plot name
67. RelResp Tot RUJ Fast Fraction 96 Slow Fraction 96 ty Fast s t172 Slow SJ slow Fraction ty 10 t Plot settings Finish Biacore T200 6 PERITA 175 6 15 2
68. E Lomeli ai a A nat m m pgp 1 E Pd rh Sample 8 ohti Serge Coar rop eni Tai 8 Disitiun Lm L atu i Targ ET J Tatag apn 4 nar en Varas m pio Em licen a Cair wer hec i L abi r furi B mir Fa rm hs im Wil rl yn nn rril ampia nnhn i 13 11 JFE ni i iF ii a el n pem Scr 24 X 0 Next gt Biacore T200 50 3 tb 569085 c c ELI ODB KHOTT Ed chr Eis mmn pcapacks 11545 ei Hin er capackp dA C Depi Pio papacfp 114 C FeFr Pr cns EiPsmnFT 114 Colo Tr Fm mm Eee jid C Deor hh mm capei a S C mpi Pin mem DApacf 1 amp 4 C mgr HiL em canal 1 amp 6 EE JI Z4 663 mrj ILI ROO 0C IN COPHET 3 FT pm mpr Fin mnn CApACE jid C Lmgtr Fi m rApa 1bl4 C Leges Flux mmn DmpacE 11 amp C FT PE s PTFE i 200009000 JOOOOO OOOO0000 4 0000O0OoQ JC um weeeoooooooo ee500000000 m 1 00 Leer mie cns Caec Dih4 Cone rr Ph me chp ecke jid 02 acre Fh mnn rapack 14 LE unn 240 LE 501 24 90d WEI mr 6d iid ET LES m m m 90609 60000000000 000000000000 0000 0000C ul
69. l Biacore T200 78 3 ka Hrihad Horihiri Wam melb i Sampe Sample solution Flow rate pl min 5 ul min 100 l min 2 Mw Da 7 3 tB D 20 C 20C Dilution S v 7 JLO0 7858 58 Biacore T200 LT Inns EAD 1s ana abun a y c Dn rem kr Blank Flow rate ul min Sample Next gt lime mt pham mm ampm asque P dbem currui a mF ah tes Cre 1 a up mira F Har car iom E my ur i dria la Tak LT L a Bari Bur T D d TENIH ii F uum bal ib LUE Jas umm ii L PUDE in d psit king ii E epa m h li HITS EETE tm dl im LLL mim L 18 Fap TE ubi idi am amm ph NW m ET EE ta mi iii In THEE Ti zi lji unam hib E ERI Lbcw iji EL tem adm bas Gi LEE T imin ti ai Lg Li Lue 1 m JL m Biacore T200 80 3 Next gt Tu hei ir iyii I rm
70. Chip Chip type Capture 2 4 Sample Regeneration 1 or 2 Carry Over Next gt Yu le rr cl re lup x EE FT Bar orere Fm TLF LE TT a ji timia cp aec lus gi asta c pce i Jm Mursber gb l ur aii hm mmo Uo gt LEDin zu la duh EEG Lamp roltpames enmtpes gang o Dim re E k NN Tr nes act Conditioning Solution BESRA Te I ER BD OD EA A Contact time s Biacore T200 Number of injections Startup Solution Number of cycles Solvent correction 4 87J 89 V2 5 90 8B 97
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76. MW correction Plot type Report Point vs Variable Axis setting Y Report Point binding Response type MW adjustedresponse rmm X Variable Cycle number TCT Fer hm i Fu maman Ha MEE lpm e Eri h og rt Fuse Bom mAg rem E i Paai Pomi fr mrd anam uw x Fammi Tp 3 a ed a Finish Biacore T200 Evaluation Explorer D Plot Pot Work area v 100XRU Da aurem Mama mm EE EE 1 2 41 Wi Cusin Fr i Ramia Searg arn J AUI pengongramg a Bril inpe ai Drie level a eina atbil Fm ln irlrrrm TTP FE Fr breira 4m Ehret Crecbo EE Dixi Step Papa CDrerlr fe te Cr ambe cento eja elem zpom Leck r TH H 0201 ULEF 1C 156144 11 Dz31 ls L 537 13 3 550 14 1 15 1559 amp D 2122 15i d x3 Id 00n A Oa zl
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78. 7 000 RU 15 000 RU HBS EP 6 127 Merged Inject NSB Reducer BR 1006 91 Merged Injection 1 mg m HBS EP HBS EP 150 mM NaCI 0 05 Surfactant P20
79. AbsResp Baseline 50 RelResp Baseline RelResp Binding level Evaluation Explorer 4 105 Hee d5D DREE Ko kk Biacore T200 Toolbar Kinetics Attini EF ET LF rm G geeet Cipre i yl Nx al al 3 X Xi A A F ree rm Von arid l fon avag bari Heb j Meine EE Select Evaluation mode Single mode Next gt Biacore T200 106 4 0 Kinetics gt Biacore T200 4 107 Mode 1 1 Binding Fit Kimea a Ainii FA EI
80. Include OK 3 18 CFCA CFCA CD Initialrate RU s 203 15 RU s lt 0 3 RU s gt 15RU s 2 QCratio 0 13 lt 043 Biacore T200 3 89 PMP tuve acer tm pre RUA pred CU Dai v F hit E Lon LS F Frm4 ni he pME O me Ya CD 1 39L 25 EODD v l E 4 20 w Ja Lon 2 Pme 100g PT A SD conte a Cn i1 CO 1 ful z ra 14 5 If 15 Lon Z5 Include
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82. Cycle Run List Next gt d n sae de asc LIET iei 11 P0000000 DOOOOOOCO lux DOGO COC s G9960000 lli DOQQ JO m JI 1 UO CC qme OGOL JC JC i ma JC Es a Ww C OO je CC LJ rt X D E L jJ b b Biacore T200 6 159 6 9 Menu Export Positions Menu Sample Position Import LET nunaperi
83. Biacore T200 6 165 6 4 ADAs ADAs ADAs ADAs ADA ADAs 6 4 1 Toolbar Run Wizard Menubar Run gt Wizard ria pares mieni Tap ine mE aj pulss Piaf nn HER re T y msn pil Ge Log i Fri Aud T opii EF z Lambro Ei dirim Dia ie Lees zl Fiere rej ze Pal Ful 5 arr E Er reg g Fular Fn zn zl er Egami EE z ET ga Faroe a Trim 2i Asp z Erais rh Edna Areir 2E cvranerd seen D rat it Fhernocreannet 23 Dp ri vm ceca Conima ar urgay rg En R Screening Immunogenicity Immunogenicity Screening New To debent irib nm hi eag m Fun pam campi
84. Evaluaqation lN i tE 4 5 Keyword Table Tools Keyword Table ETE e 968g WFamsmEmSxsx AA nacrzhmpmudss et usRh amp EkrcE Biacore T200 104 4 Menubar Toolbar Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer lil ouram amp oio Baseline Binding level Binding stability Binding to reference Work area Biacore T200 EOUME P mm mam Sensorgram window
85. T i p z d i i NH n a EIT 1 L E P I v E E i SC E W Ooooooo0O0o0 QO E Gerri E hm Te LLLI be ai e obis l y lej a ha lbp iial e j le bi oa pael E LII d E her dem hem mask SUUS UE HD DET Bnr p 009 3 Eua Pi FI el QU GUY s himt chupa s m E l dg e Ru we pM PITE E FE m ng gt domum s gt Bap 5 cg iiim d o CE ial ahe mermi i e hr L imr r Baia i 5 fum om i E i iu i i booling Auto Poolingo_ Yes OK Automatic Positioning Biacore T200 122 5 68 7 72 24 ony Eject Rack amp 2 U v 2U C Rack tray port 2wW2kv 4 8sx CIBAUL OK Eject Rack Tray Rack Pos
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88. Control Sample R1A1 H5 R1A12 12 E mnri ppn is mum lim ib ri habit Bp j T E Tice fg imc ies rm d amiri Hi LII 3i p dem tm mask Tin zm is B Em E Spa NP Fx EH mm g era z F BE i Ims eH pda zo m a m m DUE Lane a fim d m zm 1 um in IE moa I et g amp l mi Pooling Auto Poolingo Yes OK Automatic Positioning Biacore T200 LLL LA 101 Eject Rack
89. Use reagent s per cyce Regeneration Next gt Immunogenicity Isotyping Setup Flun conditioning cycle Coniscthm Mumbai ol nischer casu v Bun satu eyuk E nluticrr seum Hied 3 Next gt l Biacore T200 156 6 er umane rie Hy Feulsnpiimg In peciam Fiiil ers F ES i Contact me E h Fmre ss felm biit ritas Conad ume DO ll Gima 3 mm Flegenerancn ete Timi To pH HADH C Hugh yepi mia rt bre x ll Fire mia O jebie n p U ial e Epua sah after insecion si ET TL II Heb chak mee ps Merged Inject Sample Acidification solution Neutralization solution Next gt l 58 ims up eicity kotypirp Smmplee EJ pir PE a tample id TEI l 3 ja 4 ji B M Es 2 a B w J uk lm as a lm 82
90. two component mode 2 R Ri x e kKdgf t t0 R5 x e kde t t0 Rit 5 ka kae Ri Rz kai kae ty tla 5096 X cs ty In2 ka Biacore T200 A ALAE TTE 125 126 128 144 152 153 154 165 AGGNSOIMGINCOIIGGNO 36 AdIUStMeNnt Tor COTOS TETTE 14 UDBISEMD audae E N Ci 69 114 Ahalysis termibe CUTS d dese lere Abtei bb he baa bes beoe S 61 49 97 TTI9 ARNOUD ANUDOQI ES PR S 125 ASS OV SLEDS aasian i i E RM 69 114 B BOSEN coectetuer dudes Duet eo 11 35 42 55 86 104 140 151 163 172 BHGA EVE kena T EET 11
91. 1x 9 600 ul 100 96 DMSO 400 pl 10 000 ul 6 DMSO 1x 9 400 ul 100 96 DMSO 600 ul 10 000 ul 4 96 6 DMSO d 8 DMSO 496 DMSO 100 200 300 400 500 600 700 6 DMSO 700 600 500 400 500 200 100 700 700 700 700 700 700 700 700 yl Biacore T200 2 23 2 1 Toolbar Run Wizard A Menu bar Run gt Wizard Assay Kinetics Affinity New Methods and Templates 2 Open Browse Open Te Pisik FT HI
92. AbsResp Binding level Baseline 73 50 RelResp Binding stability Baseline RelResp Binding to reference Binding level Work area Evaluation Explorer Biacore T200 164 6 Toolbar Immunagenicity Ws Isotvyping Isotyping mm T ll i ET Curve Sample Cut Off Cut Off 2 lgM lgG Cut Off Finish zz Cut off 2 2 Cut off
93. Double Mix 4 160 a ik 162 6 4 ADAs OD DENODD E 165 E EEE AR Lar IDE ERE TO 165 OUO IN uec ASQ E E MARS EAE ALME EE 171 Biacore T200 1 1 A7 1 1 Toolbar Run Wizard 4 Menu bar Run gt Wizard Assay Erietics AA EiniEu Binding Analysis Concentration Analysis Thermodynamics Assay gt Binding Analysis New Methods and Templates 2 Open Browse Open Es ic nM Tip Inh sn et E LI REGENERATION 1 1 8 ERAEU S
94. Hee Heru Start Save as Methods and Templates 7 Bia Users Don t Save Save in File name Save Standby flow Biacore T200 Evaluation Software HB Biacore T200 150 6 6 2 2 m 9 BENT Evaluation AE 6 7
95. T Es un T Lm n F al Ela mor mo Curve onip a emp Cak Cmm CY Eon re ei ki Dp G rp mb 59 Biotin 1 1 L4 Lx T o Teak na aon l i E 1 FHE FRE a Ha aM im 1 IB uam i a ti cm ES L L2 TE Ti wa ia ia E mx i ii i noa u rx lei MA ia Lr 40 3 fl Mua mw B ma Aiea IG Mia MD Ale H AF LUL z E T zz Chf 1 d T i n i Ei i im Is I Ce 40248 d ule a yt E E i Heb Fe e Concentration Analysis Create Calibration Calibration Curve Settings Flow cell BENTIC AIF S tJLOGXE R Report point Response type Relative Response Slope Fitting Function linear 4 parameter Use preceding calibration curve Use average calibration Curve Biacore T200 Use calibration trends
96. 86 3 Menubar Toolbar Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer MERIT piot Baseline Binding level Binding stability Binding to reference Work area Biacore T200 AbsResp Baseline 50 RelResp Baseline RelResp Binding level Evaluation Explorer 3 87 Toolbar colis RN o y OL P m Tg Bs Eui wullidfit i m LU pos i Pile 7 pared Hoi 7 he re eee er pes mid carin TH Tm deHiabrate Cra lactor pimin prel pAl s prel bow Oain A FH D mouse an GERADE Em iugi fenem A nH LO mese 35 OL ITE ei Cyc
97. Concentration Analysis Create Control Samples Add Biacore T200 62 3 ww hd Ponen fa ppm pori umcor h aem mk i Darussa mee Fr Iz moon pont T OE CE X Te EE mss m n C dpp 1a Selecta Razas mer pupa umi MART arp Fa Lm tup Caulis atr Control ampie Samum Id Cycles OK f Custom Raport Panlniz Customlepost Ponti n um F Vt abl d J ec te chop o Ct mm Calibaration Settings Report Point L alibration Curve Settings Flow Cel Fc21 Report Paint baseline Use average calibration curve stabilit stabilituz Biacore T200 5 2 TEE TER AI Ee CD ee S RIE CFCA Calibration Free Concentration Analysis CF
98. Inject and recover Open Method Builder Main Overview Biacore T200 Biacore T200 General settings Biacore T200 CD Data collection rate 1Hz Detection Multi 1 2 3 4 Sample compartment temperature 4 45 C 25C 4 Concentration unit Buffer settings After run Assqy Steps Biacore T200 Surface conditionin
99. Run order Next gt Biacore T200 6 133 8 Immunapenicity Screening Cantral Simples v Repa conia Em amv li ruri la Run control samples Run control sample s every 10 20 Next gt T laamimnnpnrmiclty Screeedng System Frnparatlans X free betons run Hapse decir TERERAA wira Aisha Arig E TU rci jomple cormgpeat bcuerd eemgpsei ntur er nr Heb lCmwRmlm cg tee oem Analysis temperature Sample compartment temperature 10C 10C guanidine HCI 10C Cycle Run List Next gt Biacore T200 134 6
100. d 1 f I I 2 9 Keyword table CAEL Zg Tools Keyword Table 3 e E i i T BIT ia panee m d dca WEES2ZSES EREREIERBEBNEH IM IHAHA MES NE 5 UE CN pu UM s EN A XEREENE E k Biacore T200 Menubar Toolbar Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer W nsorgram piot Baseline Binding level Binding stability Binding to reference Work area 2 35 t op rurfum oe ee 1 UO Tuned Em Sensorgram window AbsResp Baseline 50 RelResp B
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102. DMSO 1 1 500 RU DMSO DMSO 10 RU 3 100 RU 10 000 RU DMSO 3000 RU DMSO Biacore T200 22 2 FIE 2 2
103. Rack tray port OK Eject Rack Tray Rack Positions Next gt hm HU Th DT diede Chp ri decki Wib piure Hi rerep E reagent are aded ath rack aed minapi ascpnbng ip iba Rark Pan rm uriy Die padd bs veaied with eabar caps and mazroglaie sh iharra eal Puis ha boiardi im lfm b hiad ram ind mbar Tos omeri Tulgo bie biben HE TI TrmmT HH THT the mata bortie balcone vigi pf The ngn imei guid Th jer bestiae enndinona ma emerdi bampesanuas Chanel and iani Noe ECK VGIERASIB MWERA 5EBERLLBL ULZKESBL amp SSIZmcfsd4 start Save as Methods and Templates 7 Bia Users Don t Save D uwdct Biacore T200 102 4 JJ E89 V2 90818 Save in File name Save Tr drama sri
104. 3 m 3 Next gt Biacore T200 132 6 59 ImTimnmrmweilihitm S neiening pectin Parameters Conimct me DES i Fr lere Acid colon 120 nid HC TE P Meualliebon solution 1 34 TRIS DS FA Erfurt aid subo hemp antr aobs ig ar et RT s own 5 IgL rei FmH reracrm Jp ET 1 5 4 Gs SD mh FED RE vun onmi BU Wo Fkerrae 0 i eieaa a fi a a tah ket 1 rrd Pi Hyh erect abn rt 1 Wio Pierie b Ltt ed D Ir i Esa raih lm irgecton rm ZU uooropTi sse Hee ek om m 2 2 Next gt avem 1 8 e LORI dirus fanum aram 1 EU uum i enam Sarum 3 rami 2 5 ipud F 1 aser am d Hu mm Cut off blank
105. 53 3 4 Run Stop Run Hime TOC Stop Rn brechen CUPETW CT TL Mia mn Ing L hif om Ctrl Breakl Biacore T200 Evaluation Software Biacore T200 54 3 3 1 2 07 9 RENT 3 Evaluatlion 3 5 Keyword table Tools Keyword Table L um EE m ue E mp EE LT EIS Ri i n H Fin EE Fm Lu mi sd
106. 630 Enn 570 540 510 amp 480 amp 450 amp 420 390 3560 330 300 270 10 20 30 40 50 60 70 80 80 400 110 A A Concentration units Biacore T200 44 3 3 1 1 Concentration Analysis Toolbar Run Wizard Menubar Run gt Wizard 1 Assay Kinetics Affinity Binding Analysis Concentration An alusis Thermodynamics Assay Concentration Analysis New Methods and Templates 7
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108. m 2 D5158 2 QOH 2M 2 20 25 3 408 a imm a oT zu outs Biacore T200 42 2 HE 2 14 Evaluation Explorer Plot A2 028 Baseline Sample I c A o ue baseline z RU 10 Carry Over uma co binding co baseline A Startup
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110. 12 35 55 86 104 140 151 163 172 Binding to Tefererie Basan nen ne na i 11 17 35 42 55 86 104 140 151 163 172 PONOR ETIRI EE Em 69 114 C CUG CU Ve I 47 calibration BOS Nii 47 CABD ES Cn 2 24 45 96 CANOV Faa NN Iu M uu ui LL 24 42 96 GFCA 63 GNOME 26 44 47 56 58 61 69 98 114 CONCeNEEGLUON A OVS ROLES 44 56 58 61 CORE Ugo ROT NO RO ON 69 114 CORAK Rio RN ET Emm 24 96 118 COMAC UME Mcr 4 24 25 46 73 74 96 97 98 117 118 COTE a i fo 27 48 CO Pere aie RET TUTUP n 27 48 CUO REDOT PONT qns aa edant ven aa e ven ot t tn een bec bea tb t ap he ces coe ttt eod ett 18 61 UN 129 132 164 UCRUN E ec MM MEME MEME MEME MM MU MM 6 27 49 Qe aq c ue 72 116 D Pata Colection Ta ek 69 114 Bepo orn N e i 117 DepositiomsolHior VOM E aeaa Ni t17 DTEC nerian e 2 24 45 69 96 114 Biacore T200 Wiiejelej NON UNE UE 4 25 73 74 97 BI E E EE EE E E E E E T E E E T A T 20 201 22 39 113 BOIS d lt 126 157 160 E FECUROCK TOV T 8 30 52 82 101 122 TAINELE TAR EI A AN E E E E T TE E E E 2 45 131 144 166 PxC UAE CUPE SN 60 5092 6 T
111. 187 CV 96 3 7 3 8 lt Biacore T200 58 3 Concentration Analysis Create Samples Li mm imi Tigi 1 Fe i iom d re LI Ti ii dp reme i mr a4 TE ia Sr cater Poo dg TEL 19 ser cared roo iW TEE us FPH T3 d FN t IIE Qr IRL TET Uh sz n G Sample Table Dil Fact Ae CalcConc CV 1873 CV 3 7 Finish Evaluation Explorer tiaia eee 23 Fha Bemire Samele al Dinara zisbiiy A Doral aa Biacore T200
112. El Next gt Biacore T200 6 157 sil iulii GRILL ER ET I otyplm Pinar nta iss f He Gm Next gt mile Aia wep ree aede hakli drabina evaaa Sais Vrae tet F aaa FT n Muir LUN ud mi His hme E ed wi nga aen bg wem NV a a lg anti ds J anti kr Ap am hg andi lad A1 age anti Mars mui Er wal bi abi M amb ky mhi kae anii ra mui bg anti Lg ant Mg mn Mg ai Mp M arr Marea L erem Lodi mW mpm ED lind Saepe jud Lu Next gt Biacore T200 158 6 Erime belnrer run Noemalce detector emperalute sellings Analysis temperaluie as C Sample compartment bemperature 10 E Analysis temperature Sample compartment temperature Analysis temperature Sample compartment temperature d 10 10 guanidine HCI 10C
113. Gerri E hm Te LLLI be ai e obis l y lej a ha lbp iial e j le bi oa pael E LII d E her dem hem mask SUUS UE HD DET Bnr p 009 3 Eua Pi FI el QU GUY s himt chupa s m E l dg e Ru we pM PITE E FE m ng gt domum s gt Bap 5 cg iiim d o CE ial ahe mermi i e hr L imr r Baia i 5 fum om i E i iu i i booling Auto Poolingo_ Yes OK Automatic Positioning Biacore T200 52 3 Eject Rack amp 2 U v 2U C Rack tray port 2wW2kv 4 8sx CIBAUL OK Eject Rack Tray Rack Positions Next gt b B dali BEST LN Ee Fe nie decken Fm Rari Pitoni erug
114. M 59 PRUGE 9161 c P I o4 EKOE ee ALO ECB EE ep AP RR RR EU EU E EET 25 97 ee 39 F FURG FUNCION e 56 ODO 2 24 45 73 74 77 96 117 118 OWN 4 25 46 73 74 97 98 117 118 gos E A PEPETA HM 46 G EN E E E i 68 113 General SEMS cd 68 H Hi SCOsItV SOUNO P 4 46 98 MOAI i i i 130 137 144 154 160 165 ed CU i i 60 ellUde CVCE i i Yi E E O A AEE 59 TaESA i 117 K 93 94 105 108 RA i 93 94 105 108 m E E A E EE A T E E E E E E E E EE E E 20 99 94 10 Kew FB ER 10 34 54 85 103 139 150 162 171 Ene ATDIRIB eic ORC OM nou DN EE 23 a EEEL A EEA EASE EAA A EE E E A AE A AANS 63 M Merged Ine 125 130 154 156 165 MM 46 Biacore T200 N NN 5 27 49 80 99 119 NUMmber ot GVCIBS taantiacnimdndniidnncniidntuisiniddatisinteddiy eieostedeh tensa teiehiton arie edel ird iei ite indd 2 25 45 97 NNNIDGIOEINIGGNO8 NN 24 25 97 piWagier me ur ielleal m t 70 71 72 115 116 O WMI 68 112 157 160 P Hin E Ni SAA EA N AASE E A ET 5 27 49 80 99 119 R RONKI 0 RE A 13 Risiea s TSOI O ERN cc cl 117 aea t 2 4 24 45 46 73 74 96 97 118 Repeat CONDOOM EVE
115. Menu gt Sample Position Import 1 4 Control Sample R1A1 R1A12 12 Menu 73 5 Automatic Positioning E bimn Dpr ri NI ILI e gT Be Dg obis y mej a haa lbp ial e jele bioa pumi E LII Ehe i emma i pa p mash Wil nj ar Ub FS n myr Hir pi lame gt eui i FE mum Bip el QU EY s bime bep s Deui i gk EE ag s Remum ds i PI TE LD Hs gt orm m gt hyk LEUR i chem rmrmimm aie d m v al r ioii mis mi ou mal i i a IE m Biacore T200 8 1 Pooling Auto
116. Papom g hame hea oe Settings Curve Report Point stability Response Type Relative Response Regions amp Boundaries Number of Regions Boundary Region Name Next gt Biacore T200 174 6 f mia 5X Bug 2 hage aom Riu Slow values aboye t displayed Hmp Debo lad Pruelwss mmmlhram mm Fu we a Sample Rake mn ma v Dg mm p ms en rs EN 241 0 j n id pa lud pem ou mi iH mmm zal amp r i 1 al m 274 AT T E T DW ES id Slow Fraction 6 15
117. e Globular 1 2 Moderately elongated 1 7 e Elongated 2 5 Biacore T200 3 67 O Enter value S Viscosity relative to water at 20C 20C Use standard value 1 00 1 O Enter value lt 7 77 T Em Hm 3 E mu DE FR ro n PEN Bg F Lonyesrt dimTI4ERDC cogenicent Tom temperature ED U TOL UE IF T Da temperatur T 20 20C
118. ie rrer biaia od cpr Startup Solution Number of cycles 3 Next gt Biacore T200 46 3 HIE Ww rl 1 I2 Hd Conimctme dh E Hep Sample contact time Flow rate Mix with Regeneration Solution High viscosity solution contact time Flow rate Stabilization perion Next gt Biacore T200 lr Mp mh Um antiboce relin Hag selunan Tz Cancentratinm Analysis Injpctian Paramedinrs x d Ir AEN T T Fidis 22 13 d tp fru th Hah CH Hm iwise 120 ain Srab raton penod T 1 ek nen wm s umin d Fraction amp Bj 75 25 75
119. pme m nmm a ADAs Settings Curve Report Point 2 enhance_level 2 stability Show sample cycles mixed with Regions amp Boundaries Number of Regions Boundary Region Name High low 6 5 Finish Biacore T200
120. ri oum T nepr pmi gama ud Biacore T200 2 31 Save in File name Save l HERES e Eld Standby flow l Biacore T200 Evaluation Software 2 6 Run Stop Run Hancurie TZDU stop Run LU Siep ed brechen gie pee vul Ps mm Ing Uir Ctrl Breakl Biqcore T200 Evaluation Software Biacore T200 32 2 E 2 7
121. 1 117 PJA D HER 125 175 Bidcore T200 zl 20 Rus uM MM M MM E 21 22 25 33 34 36 37 38 39 97 VALE scenic E T E 22 36 37 38 ETEO EO E M ES 39 T E R un D uM E E LU DLE 38 wo H 12 14 16 40 141 TRE NC i 11 17 21 22 35 39 42 45 55 86 104 140 151 163 172 11 16 18 32 33 35 55 56 61 62 86 104 124 140 151 163 172 RR DN GC cS 18 61 Biacore T200 XD coUv duUlcla Hts g WSJAZSSUAS sSLL CES rine nad NN Cle b Ux TOSS vm I i D 9 Di
122. 41 HHHHMHHHHH 11 Ph T zi 8 4D A J 1D Temperature kak Toolbar Ter Biacore T200 4 109 E al pimi I4 Sai d iE puii SQNE T LENA am fii P 4a OE Import Thermodynamics Check Al Next gt hinan ramma HmmrFmlmmm ILrmal Biacore T200 110 4 Next gt r irani yea lari ults gen Pd 1 hes ihruwca Um gem ri riti Fergtecientert ion ommum gt Eyrie dixil 1 415 Tii 5258 9 33 1358 3 34e 5 ba 5e baa Diea 32e 122545 33 3 3358 5 34g peg 35a 3 54s X diei UT LE UT LE Panie mr H npa TT eH kimcmii s 14 LE si ima AF fia inicr e 1T RC
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124. 5 FAX 03 5331 9349 0 TEL 06 6305 4707 FAX 06 6305 3599 Q TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com O00 www gelifesciences co jp e mail Web 2013 GE
125. 737 6 129 and Sanitize SD x 1 645 95 Mire Sluis A R Et al 2004 J Immunol Methods 289 1 16 Biacore T200 130 6 6 1 6 1 1 Merged Inject Toolbar Run Wizard 4 Menu bar Run gt Wizoard d rim E puruTSe LIL aj pulss Pipsan g Dre Gl eleg z ase ore a Aci imamirmrgmri e Fiar Girej e Eum Sir aj 5udacr Perkarmonen sz LE mmmrria pen Tarip hrii Lesie Methods And Ternplales d amp Et Hare pres 1000521 050725 zu oos 7 irs zz mn p Faro Haier liaa a iry z ra rh A Erden rayin 3 Fhernoclreannet EE Dp zi inre Lonfrmaeon nmunegencey lb me Jl dem Immunogenicity Immunogenicity Screening New Methods and Templates 2
126. CA Calibration Free Concentration Analysis CFCA A f MM K s Conc dmn 100 ul min 5 ul min Mw 3j3s Da kw CFCA 150 kDa 5000 RUME Ls Mw km Conc d R dt
127. E Inpetian ti quer p 1 Biacore T200 24 2 Detection Flow path 2 1 4 3 Chip Chip type Capture 2 4 Sample Regeneration 1 or2 Carry Over Next gt LL Setup T cda Dorbi ii rabun E phys bulle Himba rf grr doen comen 2 Bun rolrrd crmteclrm Humber cb resar B Hipa xm 5 tarde ILm ma s Hm Conditioning Solution BEBAS EARE ROBEN contact time s Number of injections Startup Solution
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129. GE Healthcare 9 Biacore Life Sciences Biacore T200 Instrument Handbook GE imagination at work 1 il In 240 escudo 1 TaT Mi OO 10 2 15473 5169424 2 21 a OE EE e tattoo om ete e tet prets 20 EFIE ULL 23 po e Mu M RUM REM UM MA MM 34 Ia 43 3 1 l 0 43 CEDE UAE ED er TIU RN eo A 44 ET 54 3 2 BIR ER ODE ECRIRE eee ttti ttti ttt itt ttis ttti ttes 63 pod AE AE UD ues uester ud N LIE NrescpAM I RED eA 68 Es 85 TRE oO 93 95 UT MC i i i oi 103 E ea a A EN LE pu IH DAD tcc Mu E M E M A 112 Mop ugs c NIORT O 125 Biacore T200 CO cm OAA EE AA EAA E A T OEIS ARAE AE EAA 130 6 1 1 Merged Inject dd 130 6 1 2 Double Mix gt 137 139 6 2 ADAs 144 80920 A BID dev PAN ORE S ENERO CERNERET 144 uoo cu A eue MAE MA LL ELEM DIDA DNA UAM I LAUS E 150 6 3 ADASDP 9 7 00 elg tette te e bte cc e 154 6 3 1 Merged Inject entente 154 6 3 2
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131. LG Fa mm pk pm mma me iam e Linear Non linear Fitting function vant Hoff ppe Fitting Function Eyring Linear AH kJ mol Fr l AS Kmo TAS kJ mol AG kJ mol ACp kJ A K mal ian nna nn nRRanR Ran RRRRRARRERRRRZARREERREZAREARARERSARERARARZZRRRRHRRRHRHRRRERSRARRERHRRRRHARRRERERRHRRRRZRARARRRERARAREZRARE ZRERAREZRARRRHRARERARRRRRZRREHEHSRRREZARZEZRARER AH ass E ral AS tass J K mol 343 15 TAS ass kJ mal 22 N z Finish Evqluation Explorer Thermodynamics 2 c Thermodynamics LA antigen Biacore T200 5 1
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136. able Setup Run Variables Both Commands Regeneration 1 Regeneration solution contact time Flow rate Flow path Variable Settings Biacore T200 s ul min Both Dera b cdm e TE C5 ieira E at gram pomem mua om nam jag uc fame g a hepsi Fine rum i zampe mm a j ee r img j p er ELITE TI am cube exi hae nmm lm mmm mm Tm m Assay Steps Sample O Define some values in method and others at run time 5 Setup Run Variables Sample solution Flow rate ul min 5 ul min 100 ul min 2 MW Do 7 3 8B D 20 C 20 C 2 450 S SURE Dilution Blank
137. ancement 2 Biacore T200 162 6 6 3 3 Evaluaqation EE Keyword Table Tools Keyword Table IN z dm Ha 22 m mu Puga f a E m IIIHIIIIEIIHII IIEIIII ITF E HHR FeS d pucsmEE rrt Ifi Biacore T200 6 163 Menubar Toolbar E T Sensorgram window Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer 0 llot Baseline
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143. g 2U v 2d 2 Number of replicates Times 3 Inject and Recover Biacore T200 Number of replicates Times Cycle Types Biacore T200 Inject and Recover Um quer umm umi mm lom iran and ance 7 cpm bpp enc aie carpi rp cor mmh cr Par Hane Lore geri ui Pus Tuis LEUR Es Nen o il ma mcm mw ur ip Jem Lm Pm Fon TERP ape Dd map Pa MA sea NU LI IP LII hl a T ee ei A C Li De Sample solution Contact time s Flow rate ul min Flow path 1 2 3 4o BENI Wash solution PT 24 FZ NIIBID2U ZAT4A02 388 Recovery solution 0 I
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145. iacore T200 5 119 and Ede rete Next gt Prime Normalize Temperature settings Analysis temperature 25 0 Sample compartment temperature 25 Next gt Biacore T200 ug uu ul Biacore T200 5 1 B V 7 UL 225097 2U7 Z2 8RGE Control Sample R1A1 H5 R1A12 12 m n j Z
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150. lest Curve 0 Ligand Sample Dilution factor Flow ul min Initial rate prel RU s 10 5 QC ratio prel QC Temp MW Da 7 3 88 D m s Blank used Biacore T200 88 3 Expand all cycles D zv 2 amp de 9727 JUI88 ODIO 77V mB8IpUAHIEeZsSUusxd Use reference subtracted data l L ekdedberks 5 H Fal m 2 Fim HE zu om Lm JU e Include
151. ln DABIZ Double mix 128 Biacore T200 6 139 6 1 3 lt Evaludtion 6 3 Keyword Table CELU g Tools Keyword Table 1 IN z dm Ha 22 HTTHTI rd d FALDA E mr imn TE m ERETT F Pi E a Eg f i T a E el EE TREE EEE y I ud m puctpawEE l 7 HH Biacore T200 140 6 Menubar Toolbar Evaluation Explorer gt Work area Menubar Toolbar Evaluation Explorer illl ouram amp oio Baseline Binding level Binding stability Binding to reference Work area Biacore T200 m E LB deu Sensorgram window
152. ment temperature 10C 10C guanidine HCl Biacore T200 128 6 BER Siman Biacore T200 Merged Injection 0 12 M HCI 0 1 96 Surfactant P20 1 3 M Tris HCI 0 05 96 Surfactant P20 pH 8 5 XEO Tris HCI 1 3 M 8t L 4 M NaOH pH 8 5 Surfactant P20 1 Double Mix BRIGE 0 12 M HCI 0 196 Surfactant P20 1 0 M Tris HCI 0 05 Surfactant P20 pH 8 0 XEKO Tris HCI 10 M 4 M NaOH pH 8 0 Surfactant P20 1 10 mM Gly HCI pH1 5 2 5 30
153. mka e LEE a dou J am k paka mm Zt ow Ee Beo um ja a cmm do capt EDEN i ami Rue E E uim A om Web ou Ak 4 aed EE LIN E PEJE LI oh mi malum Ar LI i me E i i Fm pb E i j mw A n zd d E s e mma ii F im I Eg I Pa E E m zm d Jeu hrr Bh bul BE PLE dum m ia mi PER da m amp iui ib iim m m mii zum mu F ih Ea H H bii min Hi ium iw burn a E Em STAT Tiu I d mimi om i EH ls long EN in l bua eaii a o Gm o r pn CET md Ed od j mes ig mr zi m a an Po hem ei mo cmd iid ue E mn mE LN im oro j ames mi am mil high low high ADAs Biacore T200 154 6 6 3 ADAs 6 3 1 Merge
154. ncubation time Incubation time s Deposition solution Deposition solution volume D Number of repetitions 1 10 Biacore T200 118 5 ordine si Fe prieg a pire Ey eat d cem ar Vrai ichs i ad minan Panem Pere m 4 m Roi aa CIN Regeneration solution contact time s Flow rate ulMmin Flow path 1 2 3 4 Setup Run Flow path 1 2 3 4 ENERLES Next gt B
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156. njecik n Parameters bd T mg uin Hg iia Fischen Cist vath dra ug an Paci ETm ahon okua mejana 1 7 Hh siriy ios Logntaci lame XU hk Fs AH ipi rmn Second EE mu 7 Hai eat bouton Lontact me lh Ekma lp mn Til a 2 iaj 7 Eaa veath ai a n HE ge cik Mm me AEEA 9 7 JVU IC ERST ERMA RSL Sampe Cycles without drug Buffer amp Cycles with drug Fraction Next gt Biacore T200 146 6 museum u am ogs d Ru EB mm um amm m de 5e Ee Run control samples Run control sample s every MEHE 10 20 Next gt ded gJ Biacore T200 6 147 L krerirgem reic y Dye rrer y Hrg rar prs a pte A aa spen rae Hia pases EFE Tripp cero En GE as LLLI NIE inte Baie Umm conim Coni d a
157. nk T2 AS R AH RT In kB h k e IRE AER 1 amp R in K Intencept on Y axis k i nd h AS O K mol R AH kJ mo WT Pa T K HR slope AG kJ mol IT Biacore T200 4 95 4 1 Toolbar Run Wizard A Menu bar Run gt Wizard Assay gt Thermodynamics New Methods and Templates 7 Open Browse Open 1 Biacore T200 96 4 Detection Flow path 2 1 4 3
158. rd error 10 SE 10 0 05 5 ug ml tE 3 21 Evaluation Explorer les Kineties Affinity Backspace Biacore T200 4 93 4 217 57 89 RENT kgo Biacore AG AH AS AG A AH AS 9
159. s Sample Settings for Sqmple1 Buffer Type High performance Contact time s 36 Dissociation time RBS s 5 Flow rate ul min 30ulmin Flow path Both Sample solution Buffer Commands Regeneration1 Regeneration solution Contact time s Flow rate UMmin Flow path Both Biacore T200 74 3 D Cycle types currently in Method Sample Commands Sample 1 Settings for Sample1 Type contact time Dissociation time Flow rate Flow path High performance s 36 RBS s 5 Is vari
160. s nop Dan acne h omm ron Fun m bcde wd mn nmijpqmcm rrei bner uma rd Hos dz por eri ba paricrm Far cun quamemend EE TE O Direct analysis AEON TA EE Inject Next gt Biacore T200 166 6 EAMHAIN I 1 Enhancement Regeneration Next gt Biacore T200 6 kaeh hwr mn Ei ln Paria Mea 5 mm Heen par Tokien 18 esed Ria High zxccary su prisci ume 30 ml Sin gaai Is v Epic mh ain rct ih S mol PI Be Buk He ps 167 120 Next gt E iimiurmiperm ET Shure hrs I A d OMA E a a m I Run order Next gt Biacore T200
161. tion Number of cycles 3 Biacore T200 1 3 Next gt Sqample 4 mnia 3 Biacore T200 4 1 Ta EID M DTP lr UTERIS T ILI OECD Conie hne 1 im dmn Dexocanime 19 T aca un ace T Tima Fil HE L High eect iiion Conin dme X Fem X deben SisbArsbn ped Oi kek Bes De Sample Contact time s Flow rate l min Dissociation time ERES s Regeneration Solution High viscosity solution 40 Contact time s Flow rate l min Stabilization period
162. y solution 40 Contact time 60s Flow rate 30 ul min Stabilization period Os Next gt Conrrnrau Core mod atn iii n p sm Amm P LE DESI nod ein nd D 2c0w 1 T 3 i E b T LI lrg natn come aon Sample id MW Do Concentration nM ug ml Next gt 4 1 Excel Excel Excel txt Biacore T200 4 99 bep Cem mur iii anus 1 1 oi ELE or JE ri r IIE aup
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