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Zona Infrared Laser Opyical System（学術研究用）

Contents

1. 142 ZILOS tk Performing Laser Zona Drilling Videos Sta
2. 200 pjoule 300 mW lt 667 psec 1 2
3. 134 Kanyo Konc 2003 Han et al 2003 DAY3 2 Chang et al 40 DAY3 ARIZ 322
4. Crosshairs Report ASCII Image Capture Video Capture Video Devices Audio Feedback System E 6 10 DORN FLIO ERE E E Enable 1 ENABLE 2 SAVE EXIT PARNFRL IRES IKEI TENI 68 ZILOS tk Chapter 6 The Setup Screens 1 ENABLE 2 SAVE EXIT
5. IMAGE LABEL 3 IMAGE LABEL 4 cHANcE FONT 5 Ok FONT 6 OK IMAGE LABEL Draw Ellipse DRAW ELLIPSE 1 DRAW ELLIPSE 2 3 4
6. Class 1 Class 1 Clinical 15 Clinical 1 40x NA 0 60 IR EF LU P EL aN OKFLY AINE BEGEELIMNLIENE
7. ZILOS tk LFLELOEODEEMLIFSE DEEDS EELDE A BELTS TREDBVESO COREA CORALE BOLES 1 2 3 CPU 4 KYKD ID OFF PNB 9L FP OEMBESL TEDE A
8. Video Device 1 SETUP VIDEO DEVICES ViDEo DEVICES uss 2 0 A V CONVERTER evices Setup ca WARNING eee Changing the video device video resolution or video rosshairs 2 P 1 ibrati i i ane Cai display type will require recalibrating the magnification for all objective lenses and realigning the laser target Audio Feedback System Video Devices USB 2 0 A V Converter al Cee Video Resolutions 640x 480 Video Display Type C Fill Video Area lt Keep Original Size Video Frame Rate Camera Input ear 37 EO IV Always Attempt Input Save Exit Cancel EY D 1 Video Device REX 1A USB 20 A V CONVERTER ZR VIDEO RESOLUTION 640x480 VIDEO DISPLAY TYPE KEEP ORIGINAL SIZE VIDEO FRAME RATE ALWAYS ATTEMPT INPUT 2 3 4 5 SAVE EXIT
9. TXT txt ASCI TXT TAB 154 ZILOS tk Appendix C Database Communication ASCII File Export ZILOS tk SETUP REPORT ASCII REPORT AscIl Report Saving Options c Report Only Report File Folder Report ASCII C IVF JPEG Files C 3 ULAN hKO RAAF 2 IMAGES DATA 4 IMAGES 2 4 Images in Report 2 Images Data C 4lmages EY C 4 LAR KO I IYER nore LARK AIZEA SAL ROA
10. Low MEDIUM 143 ZILOS tk Performing Laser Zona Drilling Eyk PARUI ENRE TARGET TYPE RREI EE BIFNE OMBEILE Fink VAT t CNRERONLABIIIELCT L Y HE HARA Vb II OBRALRINE DHERLET 1
11. RED i ZILOS tk RED RED wera THORNE Closs Laser Product MEA LAA yy ORRA s n e AANA WY he i BY 1 6 RED I USB RED i USB USB G USB
12. Clinical Validation ZILOS tk Laser Clinical leg Medium High Hide Target Measurement Toolbox Tool Tvoo E re oat Video Record and Playback IE Time Lapse Mode Koa Capture Overlay Auto Capture on Laser Fire ORKER 5 2 Clinical Clinical E KFFOALY KO NRL 52 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface MAN menu FILE SETUP VIDEO SETTINGS OBJECTIVES TARGET ZOOM HELP File Setup Video Settings Objectives Target Zoom Help Bl 5 3 Xf 1 O AE File FILE
13. 1 1 2 mm 1 2 sETUP LASER SETUP VALIDATION MODE TARGET TYPE CiRcLE TARGET ALIGN TARGET PULSE DURATION 3000 us max 6 8
14. LED Status Laser Controller Connected on Com Port 1 Bl 4 20 LD Iy Aa 3s O KBR E 1 FILE EXIT ZILOS tk ZILOS tk Windows Task Bar START SHUT DOWN Windows SHUT DOWN WINDOWS SHUT DOWN NSALOBRODNSEE CL HADDYES Windows EL wk EFI AV IF EPL T RRA TAC BCE 8 TILES SHS lt SESE OTF YI SEO HBILPY ALE CEA CAEEF CIE A AIYFILKS FRAME T Itta 49 5 ZILOS Tk
15. 40 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface 4 9 8 NOE HEIRL LV AO BGEDAFOGFLAEBAILF EELDE 1 USB
16. Microns Pixel MEASUREMENT TOOLS AEE IED CF PUIL L aL OREITIA 1 oa a N CAPTURE MAGE IMAGE THUMBNAIL GALLERY MEASUREMENT TOOLBOX TOOL TYPE ZONA START 100 CALIBRATION FACTOR 104 8 ZILOS tk 8 1
17. 0 5 E 6 5 FARII RT FA COAOVRRIKEILR EEN TOBHA NOTE a we Clinical LOW MED HIGH DL S AMO F IVIL AWEEIERRLEGWE FRRULYTISRRANEF CA 63 ZILOS tk Chapter 6 The Setup Screens 140 C 100 C 80 C 60 C Violet 50 C HK 8 SaGRVLYTIOAF 6 6 6 Iv 140 C gd 100 C A BC ly 60 C ly 50 C El 6 6 FRRULIORT ERK E 6 7 FREOF aR IPAE RTS
18. 1 Day 3 10 um 25 30 um 500 600 us HIGH 2 4 1 2 Day 3 Day 4 HlcH 1 25 30 um
19. 9 ALIGNMENT Ok NOTE F EF REGIE RALLT ZEL RED i 1 1 2 2 HEX KNOBS X X Y FF Oo NS 101 ZILOS tk Chapter 7 Target Alignment and Magnification Calibration FIASTIFE Y HASTY Eb kity h ERED RED 3 X Y RED 4 RED
20. IMAGE THUMBNAIL GALLERY NOTE CAPTURE OVERLAY E AUTO CAPUTURE ON LASER FIRE OHA OFEREFD TK HE ILLEGE SETUP LASER ORE CBAC BLANKING DURATION OBE NONE LL TACBHEMBVEGS 65 N S EA Report Image REPORT IMAGE REPORT 2 IMAGES DATA 3 4 nore HT DEFBOLA L ARKA SNEER IZA YE CA HHAOBH 4A a 4 EY 8 14 Report Image BE AF L IMAGE THUMBNAIL GALLERY e IMAGE CAPTURE IMAGE THUMBNAIL GALLERY NE FEIT BIL FIFA ED SOAFLPTIF LPI SCE
21. 22 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop E 3 5 E FMB OAXSIK HIDEIPEN LC VOLK PE O EHAS E BUBOK GIP AE C 1 2 HTB part 301158 3 4 3 6 LY Bl 3 6 23 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 447 1 RMS Nikon Diaphot RMS Zeiss Olympus Leitz Fluorovert RMS 2 Leica Leica DMIL 25 Leica DM
22. ZILOS tk FIELD HEADERS ZILOS tk FIELD HEADERS 150 Export File Field Headers MelloWood Medical IDEAS BabySentry RecDate SMILE ZILOS tk FILE NAME FILE FOLDER SETUP REPORT ASCIL ZILOS tk mer ZILOS tk A mer FIELD HEADE
23. 1 2 e Bo BEREL VR TAIPETI ECE FASANDDYIET COBOEICML Cid HH EOREERPEUEDELET 1 2 C 2 3 4 3 C 3 5 4 NorE 7 5 OR BIL TE EEIN ZILOS tk EARE FAS F BERLMGITSALE CHEF 5
24. BY 1 2 ZILOS tk ZILOS tk 1 3 ZILOS tk Chapter 1 Introduction to ZILOS tk Bo SANS EI 1 3 USB 2 0 EJ 1 4 USB USB ZILOS tk Chapter 1 Introduction to ZILOS tk C
25. PAUSE LIVE VIDEO FOR VIDEO SETTINGS PAUSE LIVE VIDEO REES F SIME 1 PAUSE LIVE VIDEO FOR VIDEO SETTING 92 ZILOS tk Chapter 6 The Setup Screens 2 SAVE EXIT PAUSE LIVE VIDEO REZ A DITT ENIE 1 PAUSE LIVE VIDEO FOR VIDEO SETTING 2 SAVE EXIT Video Resolutions 1 D 2 SAVE EXIT ETATINTR PE TAMPEREFEALIEGE SMYLYRAOFLYIL A ee Video Display Type e FILL VI
26. nore Lumenera camera FIVE KANEGBIA HITORECILAK JWUl LBA CUFF A Lumenera camera FMI TIL AKL EFT 2 CCEA 77 Lumenera Camera E YAK NF ENIE 1 LASER INSTALLATION CD 2 USB LUMENERA CAMERA HORA USB port 3 CAN WINDOWS CONNECT TO WINDOWS UPDATE TO SEARCH FOR SOFTWARE NO NOT THIS TIME NEXT 162 ZILOS tk Appendix D Video Settings amp Resolutions Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software O Yes this time only Yes now and every time connect a device No n
27. 10 Laser Adjustment Screw Laser Adjustment Screw 10 11um nore ZILOS tk I L P DP4 AKOMEUMRHEAADK BE CH MANET A fa DS5OHHCIN TC AIOEGBIEZ LU FOFIE o CL YP FI4 AROUL BFARBL ESL Lb P EG IETEN E EDEA CS AU L P HARE TRE ROR RONG OTWSGCEFBRLET LASER CONFOCALITY O ABAITIE IL ARMIA T BHEE ERSTE Sb 1 s 160 1 e Mark A Lot HTB part 3008055 2 3 2 4
28. YET Ad GlobTek GT 21089 1512 T3 GlobTek GT 210097 5012 IL E 3 14 F 30 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop USB Bl 3 15 VIV BAOH T 31 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop FIRE BUTToN REMOTE FOOT SWwITCH 3 3 16 e 1 e USB 2 e USB 3 1
29. CALBRATION FACTOR CALIBRATION FACTOR CALIBRATION FACTOR BMP Version 5 1 ZILOS tk IMAGE DOES NOT CONTAIN CALIBRATION FACTOR 149 ZILOS tk
30. AUTO DETECT COM PORT FEA CERISE 1 COMPORT 2 SAVE EXIT AUTO DETECT ADIR REEDA 1 AUTO DETECT com PORT 2 SAVE EXIT 60 ZILOS tk Chapter 6 The Setup Screens Mode ZILOS tk CLINICAL VALIDATION CLINICAL 8 BG VALIDATION 9 A Validation E FIZ EADHITIL EA CEE CAN LASER ENERGY 8 1 CLINICAL MODE ERRI EIIE 1 MODE cun
31. 171 ZILOS tk Appendix E Using Shim Spacers NOTE At POLED SIES CH LL RN Y IEBELIPS 7 4 A Elt TEE CA 5 50 um OBE 0 002 51 0 004 102 0 006 152 0 008 203 0 010 254 0 01
32. C 19 ZILOS tk 3 1c 3 1b 1 4 3 2 3 2 3 3 E BJ 3 1 a ZILOS tk ZILOS tk c d 3 2 YPLAA a lt AV Ab HAF c RMS EBA d FLAK e 20 ZILOS tk Chapter 3 Unpacking Installation and Start Up
33. ZILOS tk RADIATION WARNING ZILOS tk 15 ZILOS tk Chapter 2 Safety Precautions and Warning
34. 2004 Miller et al 2004 DAY3 McArthur et al 119 53 2005 Kokkali et al 2005 2007 Han TS Sagoskin AY Graham JR Tucker MJ Liebermann J Laser Assisted human embryo biopsy on the third day of development for preimplantation genetic diagnosis two successful case report Fertil Steril 80 453 5 2003 Chang CY Lin YC Huang FJ Kung FT Chang SY Modified laser assisted zonal opening method for human embryo biopsy in preimplantation genetic diagnosis J Reprod Me
35. um A 4 5 MEAN BE sp ST MISA E ECOMEBRNT N CEBLDIt CltbYEU Aro MEAN MEE SD EDER CL FIL IONE FILSBHANES cLEAR ENTER REPORT FAHRITEOREGEL K HKHIBA TEINI 1 2 REPORT IMAGE 1 2 3 4 155 PRE
36. COUNT ROOT NAME RESET RESET couNT count AGC ROOT NAME ROOT NAME Multiple Image Storage Save All SAVE ALL IMAGES MULTIPLE IMAGE FILE STORAGE IMAGE THUMBNAIL GALLERY SAVE ALL IMAGES Multiple Image Storage Save All Manual Ask for file name C Auto Use Report file name lt Auto Use Root Name Root Name Count MultilmageRoot p 4 Reset BE 6 25 EZF ILORE JRF Manual Ask for File Name FA Ax CORE TPILBFBEAtL sav
37. 1 44 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface 8 4 14 9 10 4 14 AE F GlobTek GT 21
38. 0 5x C C C 3 e OEM 2 e R 3 R 4 OEM 2 HT 301250 C RDI PERSE S 3 Lumenera
39. FiELD HEADERS FIELD HEADERS ASCII File Export File Name ZilosE xport mer File Folder FwF Data C 2 ASCII Field Headers ZILOS tk FIELD HEADERS Patient ID ID Number Name Text Embryo_ID PN EHRE ID Number 152 ZILOS tk Appendix C Database Communication Report_File Name Text Date ddmm yyyy Date Time hh mm ss Time YOB dd mm yyyy Date Diagnosis Text Partner ID ID Number Fert Method IVF ICS
40. 62 ZILOS tk Chapter 6 The Setup Screens s CIRCLE NOTE F L Y BN ko CT CHER OVI REBLEED CA ARROW CIRCLE ARROw 68 El 6 4 Isotherms ISOTHERMS 6 CLINICAL 100
41. ENERGY CONSTANT Objectives OBJECTIVES 6 9 66 ZILOS tk Chapter 6 The Setup Screens 1 OBJECTIVE INFORMATION 2 OBJECTIVE DESCRIPTION 3 SAVE EXIT MAGNIFICATION CALIBRATION 102 Setup Laser Calibration Factor 0 Objective 1 z Microns Pixel Report ASC
42. SETUP LASER SETUP CLINICAL MODE TARGET TYPE CIRCLE TARGET ALIGN TARGET HIGH 7 1 99 ZILOS tk Chapter 7 Target Alignment and Magnification Calibration Laser Clinical EY 7 1 HIGH RE CL Y FH 9 FIRE 10 7 2 Bl 7 2 L P BSH iho CML OUREL EAR Yb L EID OERA LA POREERBL TSEAL
43. LAH LAB Day 5 e o LAH 16 ZILOS tk Chapter 2 Safety Precautions and Warning o LAB 104
44. Lock Nut 3 9 25 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 8 E SH BRL XOREDLIOBE AR IK IBOD 1
45. 5 6 54 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface Zoom 2 4 8 rare Alic 1x 8x E 5 7 AAZLARRSEC SIME s PAN IMAGE BETI ATII 1x PAN IMAGE 2 4 8 PAN IMAGE ZOOM PAN IMAGE STEP SIZE
46. m Pulse Settings Clinical Pulse Settings High lenn A Medium 400 Low 200 E 6 2 61 ZILOS tk Chapter 6 The Setup Screens NTE Class I L Y DXPRBFHIFST SEO BRHILKRITENENLAMO g ER built in Safety Delay I REI ERAL TFT CLINICAL PULSE DHIE RET BINI 1 LOW MEDIUM HIGH 2 3 SAVE EXIT Validation Pulse Slider Resolution VAuDATION 9 Target TARGET Target Circle C Arrow sotherms lv 140 C Ed 100 C md soc M 60 C ly 50 C El 6 3 2 T yb OER 9 7 EROGBEERT 3 1 ciRcLE ARRow isoTHERMs 2 SAVE EXIT
47. 11 LAR KOER 12 BABEFASS A ZILOS tk O1tt B C ZILOS TK iat D EFJ OEIT ILD TER GE E F G Sg USB H 20x 40x 2
48. 144 ZILOS tk Performing Laser Zona Drilling 145 A ZILOS Tk 10 37 C 50 98 F 10 60 C 14 140 F
49. uo COMBE O PILH CO RERBHERBESELONSILYLICRRANSREAH LBP LE BRLELA BHAI BETATFNIAILLOCRHRABISBEGVE Fo 94 ZILOS tk Chapter 6 The Setup Screens AUDIO FEEDBACK Nore FR GhW PHOS RF LEXAOBEIL 7 YIhwIHOBBIE ui Setup Audio Device Laser Objectives Crosshairs Report ASCIl Image Capture Primary Sound Driver Video Capture Audio Feedback Path Video Devices ican feedsack C Program Files Hamilton Thorne Zilos Audio laser way B System V Audio Feedback Enabled Save Exit Cancel BY 6 43 Audio Feedback ORE AH Audio Device AUDIO DEVICE ERRITEN 1 AUDIO DEVICE
50. NOTE i A ZILOS tk Preface lt ZILOS tk Preface STAGE SETUP FIELD GUI SELECTION MANUAL RIS e e www hamiltonthorne com s MS Word 2000 PDF http www hamiltonthorne com research documentation 1 ZILOS tk ZILOS tk Zona Infrared Laser Optical System Class 1
51. 1 EDIT LABEL LOCATIONS Image Label Layout Current Image Capture Size 640x480 Drag labels to the desired captured image location IVF Center Name Time lt T gt Date lt D gt whatever lt RPN gt E 6 33 2 3 OK 84 ZILOS tk Chapter 6 The Setup Screens A KINNOFFAK AI FRBET SIME e TEXT COLOR ENABLED Font FONT NAME FONT SIZE FONT STYLE 1 CHANGE FONT 2 OK
52. 108 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation Measurement Toolbox Measurement Toolbox Tool Type 6 enter Ea Report E 8 5 WEY ILRY IRO RIL 6 e ZONA e EMBRYO e PRONUCLEI 1 e PRONUCLEI 2 e DRILL RULER 126 SETUP VIDEO CAPTURE 89 avi
53. HELP FEI e HELP gt HELP FILE ZILOS tk BFF OIFRILP FLA F ENIE e HELP gt ABOUT 57 6 BIEREAITS SETUP MAI ZIER ENIZ es sETup SETUP 6 1 Setup Laser Remote Fire Com Port Laser Enable Enable COM 1 Se Auto Detect Objectives Crosshairs Mode Report ASCII Clnical Validation Image Capture Video Capture Target Pulse Settings Video Devices Audio Feedback Clinical Pulse Settings Validation Pulse C Circle Slider Resolution System High m a E u C Arow Medium po C 10us lt sotherms Low po 100uS M 140 C ac MM 20C Duration Black eoig o Sec Blanking Duration C Hide Energy Constant C None Save Exit Cancel 6 1 Laser ORVEH Clinical Clinical Laser LASER LASER oN OFF
54. EYES NOTE id RE THCRAF TF ERII 1000 49 16 CF FEIF EREZET Slit 1 TIME LIMIT 2 TIME LIMIT 88 ZILOS tk Chapter 6 The Setup Screens 3 SAVE EXIT Video Compressor VIDEO COMPRESSOR codec LEAD MCMP MJPEG CODEC CF BASIC Basic Video Compressor aa bia eas id C Advanced Bl 6 38 EFJ AL AIL YY OP ERE ADVANCED copgc FAP FAEEZ PHAM DEO CES REED HYVES FINGAI Y Ik copec taO TIIE Video Compressor Video Compressor Basic Properties Advanced WARNING
55. NOTE NEw 140 12 BAREIS LAH LAB LAH
56. FILE NAME 2 FILE FOLDER BROWSE FILE NAME 3 SAVE EXIT ASCII FILE EXPORT F ASCI txt E mer D2DDIFLILRY ECE REZNEJ ILFIFNKRESN TUE ASCI ASCII FILE IMPORT mer ID ID ASCII File Import File Name Importmer m File Folder IF MwF Data Import Translations BY 6 18 ASCI 1 FILE NAME FILE NAME 6 19 74
57. Image Properties 1 EXPOSURE 67 2 GAIN 1 000 3 RED GAIN 1 36 4 GREEN 1 GAIN 1 5 EQUAL TO GREEN 2 GAIN 5 GREEN 2 GAIN 1 5 EQUAL TO GREEN 1 GAIN 6 BLUE GAIN 2 5 X 10 Image Properties D MRE IE NE BERS S SEO EMRE IAI ORMEEASSEIEILBUF CAM AUTO exposure O RAIBE CZFUA H I IV RELEJE AUTO WHITE BALANCE K REORAItBEBOLET 4 VIDEO CAPTURE FILTER IMAGE PROPERTIES 2 tab Video Capture Filter Initialize Reset Gamma pe 100 Contrast 000 Brightness 0 Cancel Apply D 6 Video Capture Filter Image Properties 227 161 ZILOS tk Appendix D Video Settings amp Resolutions 5 11 IMAGE PROPERTIES 2 Image Properties 2 Recommended Values 1 GAMMA 1 00 2 CONTRAST 0 3 BRIGHTNESS 0 X 11 Image Properties 2 ORES NOTE pd CAMERA CONTROL HN VIDEO PROC AMP FILL THOTE Lumenera Camera LUMENERA CAMERA USB NEW HARDWARE WIZARD
58. e FILENAME ONLY 81 ZILOS tk Chapter 6 The Setup Screens 1 coLoR Basic colors E EE Eee Ammi e Eee FG CCC ie is Custom colors Li E EEEn Define Custom Colors gt gt Cancel 6 30 2 BASIC COLORS 3 BASIC COLORS DEFINE CUSTOM COLORS 4 ok SAVE EXIT Watermark WATERMARK ENABLED HTB Watermark Enabled El 6 31 BFIBDL BFEOLEROIK F SI E e ENABLED HTB
59. e e Marks A Lot LY 11 amp 100 ZILOS tk Chapter 7 Target Alignment and Magnification Calibration 12 6 9 Low Low HlteH 3 3 ALIGNMENT OK 2
60. 113 Video Record and Playback E Time Lapse Mode OA gt le P Browse Button E 8 6 EFAFRAERBLOALYKA N NRF 109 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation wore Version 5 7 DSIZ HIP IPAS BARVIIIN BF PAD RFSZ EY FLE FN XTOMAIPANISE FS k FAIZ VIDEO THUMBNAIL 7 GALLERY lt t FANFIDC NOEL 1 Y AGD RELET PY Image Thumbnail GalleryE IIL CI Time Lapse Mode NOTE Time Lapse E F ERAT ELONI RMILECOREEZ SETUP VIDEO 7 CAPTURE TIME LAPSE TIME LAPSE MODE e TIME LAPSE MODE Record Play Step Back Stop Step Forward E 8 7 EFJ OERZ EFTERIEN I s
61. 42 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface To USB port MUST connect directly to computer USB 2 0 port 4 12 pin E PR F 6 USB Firewire USB 43 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface E 4 12 7 9 pin D 9 pin 3 15 E 4 13
62. 2 e 1 1 e 1 7 oP A 1 RUER EGITE EK PHEZ BM RES AT REIEDDYFT AUN CORE DEGEIA LOWE DERKI DLS AT HELEDD YET FFLFIRDBEDSL No on A DN ZILOS tk LOW MED HIGH
63. 4 IMAGE CAPTURE IMAcE CAPTURE 5 139 ZILOS tk Chapter 11 Reports 12 IMAGE CAPTURE 1 MEASUREMENT TOOLS 109 ENTER F we BIL EDEFL AMPA FECES HARER REC U
64. SAVE ALL IMAGES SETUP IMAGE CAPTURE FILE STORAGE 1DFEEILTF AW TOMEGVLRIANEMRST EINI 1 115 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation 2 DELETE IMAGE DELETE ALL IMAGES NAE SAVE ALL DELETE ALL EERI SE ADIZ CASEI NW TOM BULAAT LE IMAGE THUMBNAIL GALLERY DS RURO REST BRLEBREFUAKS ENIE 1 2 PRINT IMAGE F FY ADARESSATHVA GS ENI 1 2 PRINT THUMBNAIL IMAGES 1 Image Toolbox
65. USB 179 H DUAL LASER SYSTEM 1 40x 20x MULTILASEREXE 40x 20x AS SL P E51 N L 8 ALY hO 5 KES BIL 40 20 1 MULTILASER MULTILASER VERSION 1 1 irsi MultiLaser Version 1 1 Select an HTB laser mode Zilos tk 40x Zilos tk 20x EXIT 2 ZILOS TK 40x ZILOS TK 20x ZILOS tk OA
66. USB es USB 2 0 RS 232 USB USB 9 pin D RS 232 e USB 5 3 e RS 232 e USB USB e
67. ALIGNMENT OK B P Yb RAREST IIE s TARGET HIDE TARGET RED i RED i Zoom Help Align Target Hide Target EY 5 10 RED IL EL 1 NFRAL TH S6GBOL TFyk XZ1 RED i OBEFRE ST EII 1 TARGET RED I INTENSITY 56 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface EY 5 11 RED DEREK IL 2 2 RED i 3 ok NE HR OIE VEBITIF EL VE REL Bold WE AA BERELBAOLF Fo RED i ERTI INIZ e NOE L Y EHRAS SE RED L2 PYKRRE MILV OE AHARRANE LVEF COR fel Ve REDI WARRANGZUGEE SHOW HIDE TARGET KA VADYYIL CHRTASE TEAL Help ZILOS tk
68. GENERAL DATA ENTRY LASER ASSISTED TREATMENT EMBRYO EVALUATION 3 138 ITENI 1 lt TAB gt 2 Date 5 24 2005 Time 3 15PM Patient Name Patient ID 890287 Date of Birth 105 Feb 64 Partner Info Diagnosis and No comments Comments Embryo Information Oocyte Embryo Fertilization Cycle Number Embryos for Embryo ID Retrieval Date Method Cycle ZWE C 11 2 134 ZILOS tk Chapter 11 Reports
69. CLEAR EXPORT EREDLEL 77 1 SAVE CLEAR ZILOS tk REPORT CLEAR EXPORT NOE CLEAR EXPORT I FFLILIADF B2EIFGAW CHAS SBRECS ASCH 7 FILE EXPORT DIPFANREILEDFFF FANFD HOT F HILBAUAFNETG 1 PATIENT NAME DATE OF BiRTH PARTNER INFORMATION
70. NOTE EFA ERIFELILAURS EIAI LF VIDEO THUMBNAIL DE 7 EZLOMEBADL Cd PUYILEUCEAL Delete video Delete All Videos Save Video Save All videos BE 8 12 7 UR A 1 8 12 2 DELETE VIDEO DELETE ALL VIDEOS 7 1 8 12 112 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation 2 SAVE VIDEO SAVE ALL VIDEOS
71. 2 LUCAMFLIPEXE 3 Lumenera Camera SERIAL NUMBER Boren THORNE Lumenera Camera S N Location Serial 41 UT 4 ok 165 ZILOS tk Appendix D Video Settings amp Resolutions Lumenera Camera In order to flip the Lumenara camera image in X or Y direction All programs accessing camera video must be closed The serial number of the camera must be input below Enter the Lumenera Camera S N This can be found on the back of Lumenera Camera The Serial is a 7 or 8 digit number OK EXIT 5 FUPX FuipY 1 2 ok Set Flip X or Y Select settings for Lumenera Digital Camera Flip X and Flip Y FlpX Horizontal FlpY Vertical EXIT 166 ZILOS tk Appendix D Video Settings amp Resolutions CRI Oosight Camera Video Device 1 SETUP VIDEO DEVICES VIDEOo DEVICES CRI OOSIGHT CAMERA
72. 2 SAVE EXIT Audio Feedback Path war R FFIT Ir I Wt GIRO SBE CHEEN CLES BILZ FEW IPA NECEACSESL 95 ZILOS tk Chapter 6 The Setup Screens wav DPAIWEERS ENIE 1 BROWSE 2 wav 3 OK AUDIO FEEDBACK PATH ARLE wav IF ILERE BINI e TEST Audio Feedback Enabled Auplo FEEDBACK lt audio feedback F7 VKBI F S d e AUDIO FEEDBACK ENABLED audio feedback FA Fk
73. BNc RcA 2 Video In 3 USB es 12 pin 1 BNC RCA 2 27 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop USB 2 0 E 3 11 12 pin PR F 8 USB Firewire USB 28 ZILOS tk Chapter 3 Unpacking In
74. Document No Rev F Printed Jan 20 2009 Hamilton Thorne Inc 100 Cummings Center Suite 465E Beverly MA 01915 Web Page www hamiltonthorne com Copyright 2009 Hamilton Thorne Inc All rights reserved ZILOS tk is a registered trademark of Hamilton Thorne Inc All other brand names are trademarks or registered trademarks of their respective companies A DE cesses E oa ees E E E E E E E E K s L N TIMI ELAN oe cceccccceccsesessessevevssesvenceseveusssnersevensasssacsvsusassvsueassteasavsnsacavssassnsacavsversavsnsassversesanes 1 2 EEA aa AEREE NT IEE ENERE E E E E EEEN AEA EE E NE E E EE AEN 2 Bike Be I E EL NN A 4 ZLOS tk 77 a a a LOA 77 VEBERI IES occ cccccccccccccccosccsceccecccacccsecscacesscesesseaccsasecasesscasesecaccssnsssasesseesessecaecnsnsecatesacsaeesevaucaessssauenseetesseases 12 AE LBP EL GW FEY cocceccccccccscsesesvsncnencecevsvsceveusussssvsvsvsvssncacacavavereseesasavavsvavssasacacacavavaveceeseees 12 Sg BOL I BD a 12 EB ae EE E ORO ere eS Ok POT een a ve Orn ewe ee 13 S A A y REE EEEE EEEE E EAEN EEEE AE E EE ERAS 13 E 2I RA IT i AA A kk 13 PED EEEE EEEE EE ERA EE AES T 15
75. E MOBBLUKAIL AEA DRAWING COLOR E THICKNESS FU DCTBES SEM CEES BHKUKADE RIL AHH ITO Fal 15 Draw Rectangle DRAW RECTANGLE 1 DRAW RECTANGLE 2 117 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation wor TAEA DRAWING COLOR THICKNESS BRALUKADE BIA BRIEST TS TEAL Draw Line DRAW LINE 1 2 3 4 DRAW LINE FOBALUKSI
76. 1 RED 2 USB B USB 2 0 USB BYES 2000 3000u s USB ore USB R hKD SDEDABOTAT ILGUCEDHALELE lt 3 USB CD USB USB 4
77. Message Box FA VRKEILF ENIF e Message Box EJ IRITI ENIF e Hard Drive Free Space HARD DRIVE FREE SPACE 97 7 108 1 KEW OB TEM ELIONI FIT AVA ZEELST IBRIDE Fo Lb P E LOWM EFAS FS ICABEITIE
78. SN hEOHBIL TE ZEI ZOS EAEE BEDSITICEL CEES 5 E 4 5 E C E EURBDDXSR H DGIP ENE C 1 2 HTB part 301158 3 4 4 6 4 6 38 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface Bl 4 6 LK 1 RMS
79. USBNA EES BS INVIVASAKZ EFEDLIT TCEAL e 46 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface 1y USB Ports _ erial Port Security Key ie i USB Hub j uU Serial to USB gt Converter EQ 4 17 ZILOS tk PYDKYI ORG TSAI IEE FJ IIE TS A BOVE hKF YARIAYVFERALEGES 47 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface Laser Controller Remote Foot Switch Laser Power USB Ports Serial Port i Controller Cable External Laser Objective w Video Device SAE u ERS Security Key w USB Hub Wi aa Mouse wom ih Serial to USB Converter Analog Camera EY 4 18 ZILOS tk FYFhYA ORG YARIAVYFERALEGES 1
80. amp Step Size fio EY 5 8 Pan Image STEP SIZE EZET ENIE e P RORRBMABA SIKd e 55 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface Target 7 Align Target eee E 5 9 1 TARGET ALIGN TARGET ALIGNMENT ok 2
81. Duration DURATION 0 10 FFMABE SF S K1E 1 DURATION 2 pDURATION 3 SAVE EXIT FI FIVFELDTHRAEPOILRELTE KEL ADIIT ONB ILR EO ZUH Class 1 Laser built in Safety Delay DEBET FYFAN E SOER BHAILBNEL T YK A MAG O PERL AD 6 ECE Shots Energy Constant
82. PATIENT ID OOCYTE RETRIEVAL DATE CURRENT DATE EMBRYO ID JPG CLEAR EXPORT EXPORT FILE DBGASF SX CHIRL CLE DERE CIS o e P 2 MPoRT IMPORT MER FIELDS 11 5 NOTE i IC AIK ICED 5 X 4 82 C2 DA OLR ERI BE BA 138 ZILOS tk Chapter 11 Reports Report_File_Name _InvalidDateFormat_3_22 2005 REP jpg 3 2 Selected Embryo ID Import Record 11 5 Import MER Fields a 11 5 b IMPORT RECORD ExIr 5 3 MINIMIZE REPORT
83. HT C 300936 DC50ZZ D50ZNC Zeiss 100 135 200 2 1 Olympus 730063 IX 50 70 DIX DD50NLC Part 300792 300788 Nikon 300788 TE 200 300 DD50NLC i 301269 i Leica Delta Optics Gray Paint Stripe OIA DMIRB DC50LP R 4 C ZILOS tk Chapter 1 Introduction to ZILOS tk C C Leica DMIL amp DMIRB HC optics 1 OEM 0 5x C Mount 300950 2 2 0 5x C Mount 301250 V 301257 Leica DMIRB Delta Optics 37mm 301269 Lumenera Nikon Diaphot Nikon TE 200 300 2000 TS100 Nikon Diaphot 200 300 Nikon TE 200 30
84. USB USB iF lt USB 3 10 RO USB USBF Version 27 BAUFCRLIEOD EEL CAFE A 26 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 6 e USB USB s LPT1 7 e 3 11 12 pin 1
85. 100us 10us 1 us 1 us 1 us CLINICAL Laser Validation Bl 9 2 RIEE K ROL Y LANLF IL KAN NAN 1u s 3000u s LFOUSHMODAS CNWVAWOREAITIZKEWN CEFF e PULSE s e PULSE 122 ZILOS tk Chapter 9 Validation Mode Power POWER
86. 2 REPORT IMAGE 3 4 6 14 o Date 2005 2 28 Time 8 56 4M Date of Birth Partner Info Diagnosis and Comments Embryo Information Oocyte Retrieval Fertilization Cycle Number Embryos for Embryo ID Date Cycle ethod eee eee eee ey A B E D E Number of Blastomeres 0 Meanl 0 J 0 J0 CLC o J 0 5 sD Co 6 14 2 Image Data I 71 ZILOS tk Chapter 6 The Setup Screens 4 images 4 4 REPORT IMAGE Date 17 3 2005 Time 10 34 AM Patient Name Patient ID Date of Birth Partner Info Diagnosis and Comments Embryo Information Oocyte Retrieval Fertilization Cycle Number Embryos for ate Method Cycle L JEL JT
87. 100 285 mW HALNNERE TS SINIF POWER 123 10 DOCUMENTATION Report Image REPORT IMAGE SETUP REPORT ASCII IMAGES IN REPORT 2 4 114 Report Image 2 1 2 REPORT IMAGE 1 REPORT IMAGE 2
88. 2 USB 12 pin USB 2 0 9 pin D 9 pin D USB C ZILOS tk 4 1c
89. L F EL 1 N EF DRKBILF SILK 1 LASER ENABLE 2 SAVE EXIT 59 ZILOS tk Chapter 6 The Setup Screens L P EL 1 NEAPKEIK S ENI 1 LASER ENABLE 2 SAVE EXIT Remote Fire oN OFF YE KIYIRA YF EAL KEI F ENIE 1 REMOTE FIRE ENABLE 2 SAVE EXIT YE KIYKRA YF EA TRR BNI 1 REMOTE FIRE ENABLE 2 SAVE EXIT Com Port
90. VIDEO FILE NAME COUNT VIDEO FILE NAME RESET RESET couNT count AG VIDEO FILE NAME VIDEO FILE NAME Video File Location VIDEO FILE FOLDER s e BRowsE Capture Length
91. i 101 E RLA BE AE IE EEA NEEE EE E cag ech Le zea aR die A ca hoa nana dra 103 RTA AI NS SSN SS SE NSS SAN dat oat Ratstral SR 104 0 0 E ERR ere Ee RE RR EE ne PT 106 S ae Sema E 0c AE ee RETRO ETE ERC ER RCE eee Dn ener TREE E toe en RR a reer 108 5 RMKRoe5 108 Time Lapse Mode A 110 770 Video Slider 777 EF ARI PS NE SE EE 112 EE LOEAN TEE IU FEIT EE 113 MED BIT LIE EA E E EE 113 Capture Overlay 113 Auto Capture on Laser Fire L P FEGTPF OD BERT FR oovcccccccccscccsssesssssssssesessessssstssestesssesessesees 114 Report Image L 7N KIL IB EPEAT 6ncccccccccccscscscstscsvevscscscscscevevececscsensnensvsvssvavsvsvasavacececsesssavavavavacscsenenes 114 GEENEN ee EA AP Fe ah casas hs at denotes sd pov wetted vow occ E 114 ZILOS tk X of Contents G BURNET sas gO Ee ke 2a a a eRe ree en OE eR eee 115 Image Toolbox 776 aAA ALON i OO eT PRE TROT ROTOR OE CRETE TUTTE PRUNES OC RANSON hee RET 119 Validation Mode 121 Pulse Slider Resolution VVR ROA AAO BLE veccccccccccccscscssssscsssssesssesvsvsvsvsvsvavsvavasscscscsesesvavsvevavecsenenenes 122 AEDORIE SE Ek EE EE EE 122 Power EE TI Bi ES SE S
92. 1 Report Image 4 3 4 REPORT IMAGE 3 1 REPORT IMAGE 4 3 2 IMAGE RH BA SNEER COEFF Ran T 6MGl koCThEBz an UR KORYRUA ABHALTEN LA KDABIS BRAK L FANF CA tl oT FRE 2 TAL REPORT 125 ZILOS tk Chapter 10 Documentation MEASUREMENT TOOLBOX WEYI ILEIEAS SHI FPYIL La 102 S LYBH ORAITO FRYPL vay M2 Cl SEL ECGCHERITICEIS CHF tA 6 ZONA e EMBRYO
93. 10 90 10 90 5 95 5 95 3 760m 12 340 ft 9 144m 30 000 ft 100 240 VAC RMS 50 60 Hz AC 7 8 3 9 A RMS max 3 3 A RMS max H W D in mm in mm in mm Ib kg 1 65 42 2 1 54 1 1 28 0 5 0 22 1 6 41 3 8 95 3 25 83 1 0 0 45 14 6 371 7 2 183 17 7 450 38 17 3 14 8 377 16 4 416 6 9 175 12 3 5 6 1 3 3 3 13 3 34 10 7 27 6 2 2 8 147 ZILOS tk Performing Laser Zona Drilling ZILOS tk 100 240 VAC 110 240 VAC 300 W 30 W 50 60 Hz 50 60 Hz BAT 1480nm Infrared Solid State Diode r 300 mW Class 1 Clinical Validation Clinical Low Medium High Validation 0 1 ms 0 1 3 0 ms
94. Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for Lumenera Unconfigured Device The hardware you installed will not work until you restart your computer Click Finish to close the wizard E D 10 BLL W K LP ORK II E F Finish KEL 164 ZILOS tk Appendix D Video Settings amp Resolutions nore Lumenera Camera AFIT 3 YT 6 FCOFMBE 2 ERURSAN ORADE ILS ORHILY KM 2H RRANLGAILZ LUMENERA CAMERA Lumenera Camera Lumenera Camera X BH Y LUCAMFLIP EXE CO Laser installation CD LuCamFlip ISIYI REE o THE OIMEEERS ENIE 1 LUCAMFLIPEXE LuCamFlip exe
95. Image Capture IMAGE CAPTURE 8 13 Image Capture E Capture Overlay Browse Folder Auto Capture on Laser Fire 1 2 o 4 Static Image Capture Tool E 8 13 FALL BIF Yr ZF 4 YW Capture Overlay CAPTURE OVERLAY Image Toolbox 113 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation 8 13 CAPTURE OVERLAY IMAGE THUMBNAIL GALLERY Auto Capture on Laser Fire
96. RED EARL DRE RED i TS HARL LY ROSIE Mm LORE YKOMED BARL LU REBLCHASHRUTLAIO WM BEE INF 2 TCHASCEMBYESM CMIEFLRAI FPEAAIOD PEI LBED COARI TRAPER 102 ZILOS tk Chapter 7 Target Alignment and Magnification Calibration MEASUREMENT TOOLBOX um 100 BEAR rVIL LaypRLUFCORFEL TRENERE A FELEL LV XEFOBBRELGVFET T ZILOS tk L P EL 1 WEO BE CHWGOREMEDOCLIVEG BP PRA
97. 2 3 4 ON OFF 1 48 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface Windows ZILOS tk ZILOS tk A ZILOS tk DEPIL EDY Ihr PEL EIPULNCCAL STATUS BAR 1 5 LASER CONTROLLER CONNECTED ON COM PORT 1 Status Laser Controller is Disconnected E 4 19 L aL Ka 3 OF RY eS LASER CONTROLLER IS DISCONNECTED
98. a ZIA HAA c RMS BBA FLAK e 36 BI 4 3 BELL KS Y 4 36 4 4 RED a USB KAL YF ESF ec __ 7 ZILOS tk List of ls E 4 5 E MEOIREAN C Fi GMB ODAT C 38 Bl 4 6 39 B 4 7 RALLY FCLRILN PA AEREI E 40 4 8 ZILOS tk 40 EI 4 9 QYDAYhEL OD YEO TL Y EL 1 NARESS 41 4 10 USB NF B EtFAVTI F A 42 E 4 11 PFODFHAFLINAXIO 12 pin 7K kK L bL Iy O 3 PF OF ATL SNASIGAOL RIAN yk PR 7 F 43 E 4 12 44 Bl 4 13 44 A 4 14 ALE AL F 45
99. 3000 3000 us Class PULSE DURATION Validation Mode SETUP LASER Setup Laser Remote Fire Com Port M Enable I Enable COM 2 Auto Detect Objectives Crosshairs Mode Report ASCII Clinical Validation Image Capture Video Capture Target Pulse Settings Video Devices Audio Feedback Clinical Pulse Settings Validation Pulse C Ci 3 x System Circle High Slider Resolution C Ius C Arrow Medium C tous sotherms Hts 5 100uS Blanking Duration Duration Black Shots E Sec C Hide C None k Energy Constant Save Exit Cancel EJ 9 1 Laser ORE Validation Mode Validation Mode 1 MODE VALIDATION 2 SAVE EXIT 121 ZILOS tk Chapter 9 Validation Mode Pulse Slider Resolution PULSE SLIDER RESOLUTION
100. REPORT FILE FOLDER jpg Report ASCII ASCII REPORT ASCI REPoRT ONLY tt mer ASCII FILE EXPORT 6 17 C Report Saving Options eee Report File Folder E 6 21 L hOR FASE LIR KORFAEEERT SIL 1 REPORT ONLY REPORT ASC 2 REPORT FILE FOLDER BRowsE 3 SAVE EXIT 76 ZILOS tk Chapter 6 The Setup Screens Image Capture
101. COUNT ROOT NAME RESET RESET couNT count AGC ROOT NAME ROOT NAME Still Image Location IMAGE FILE FOLDER Still Image Location Image File Folder ccapturee E 6 27 BIr IL IF NE ORE 1 2 BRowsE BROWSE BRowsE FOR FOLDER 6 28 80 ZILOS tk Chapter 6 The Setup Screens Browse For Folder Still Image Capture File Folder E Desktop r a My Documents Y My Computer E 314 Floppy A Se Local Disk C Backup Bandit Capture a cols927 lt
102. Mikon Diaphot 160mm Graduated in Microns Coarse Focus Knob Fine Focus Knob Ed E 1 SERGE OK HANK 1 2 10x 20x 40x 0 3
103. INNER RADIUS oUTER RADIUS 6 11 OUTER RADIUS IRF EERE F BINI 1 OUTER RADIUS A 2 SAVE EXIT INNER RADIUS 1 INNER RADIUS 2 SAVE EXIT SKASBILIA IEA YE GES 2 Inner Radius Outer Radius BY 6 11 DARAPRILIOUIAR 1 SELECT COLOR 6 12 69 ZILOS tk Chapter 6 The Setup Screens Basic colors El Sees ees ee EEE EEE Define Custom Colors gt gt Cancel EY 6 12 2 BASIC COLORS
104. _ gewEot 136 ZILOS tk Chapter 11 Reports ZILOS tk ZILOS tk 2 EXPORT FILE IMPORT FILE 2 C ZILOS tk EXPORT FILE ZILOS tk SAVE CLEAR ASCI FILE EXPORT 73 REPORT REPORT IMAGES C ZILOS tk
105. sp 18 1 3 BY 11 4 MEFO EZI Minimize Report MINIMIZE NEW pt IMPORT Import 138 SAVE CLEAR JPG Save Clear ASCH FILE EXPORT 73 Print PRINT CLEAR EXPORT ASCII FILE EXPORT ss 138
106. 2 USB USB 3 USB I USB Version 5 1 41 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface To USB Port tas on Computer EI 4 10 USB NF A Et FAY F4 4 FD 4 PRINTER PORT USB 5 3 11 12 pin 1 BNC RCA 2 Video In 3 USB 12 pin 1 BNc RCA 2
107. VEF MBI OIE T A b eittia TEE OEL EEE ATEI P FI AV he AEL TEE 1 Clinical 1 O NM oT FS Black Marks A Lot 25 x 75 mm 2 cm x 2 cm 3 4
108. 1 e SCREEN OUTPUT 1 SCREEN OUTPUT R TT BINTI 1 SCREEN OUTPUT 2 HOUMA 3 SAVE EXIT e ALWAYS ATTEMPT INPUT ALWAYS ATTEMPT INPUT EJA REITI ENI 1 ALWAYS ATTEMPT INPUT 2 SAVE EXIT VIDEO SETTINGS D
109. 40x 1480 nm 300mW 3000 usec CT ZILOS tk ZILOS tk Class 1 40x 45mm Nikon CFI 60 NTE AFH D ZLOS
110. NE i MEGEL TCFESL 12 ZILOS tk Chapter 1 Introduction to ZILOS tk USB Port a THORNE BIOSCIENCES 1 7 USB ZILOS tk ZILOS tk o 13 2 ZILOS tk ZILOS tk Class I U S Code of Federal Regulations 1040 10 IEC 60825 1 Am 2 2001
111. NTE COM port Target Alignment Objective 7 calibration DREME BANE 40x 2 MULTILASER 3 EXIT MULTILASER 181 128 71 2 71 ETE SIEN 72 1 125 2 125 3 125 4 125 175 i 99 ASCII 73 74 mer NST sexe ote niente 154 txt 154 95 95 95 ar et 95 114 eee 83 85 85 84 83 65 DID ee 65 WE E E E A AEST 65 BEATE D 65 141 16
112. McArthur et al 2005 p16 11 Day 5 AEA 30 um 5 6 MEDIUM 3 5 141 ZILOS tk Performing Laser Zona Drilling LD L KS FFA 1 nore 7 CRAII CL PERE RUTITICEIL A EBVYVERA
113. VIDEO DEVICES Setup scone WARNING sont Changing the video device video resolution or video display type will require recalibrating the magnification for Report 4SCIl moe PORN Image Capture all objective lenses and realigning the laser target Video Capture Video Devices Audio Feedback Lumenera USB 2 0 Camera System Video Devices Pause Live Video for Video Settings Video Resolutions Video Display Type Fill Video Area C Keep Original Size Video Frame Rate Camera Input Screen Output 15 06 15 06 MV Always Attempt Input Save Exit Cancel EY 6 42 Video Devices ORE Video Devices 1 2 SAVE EXIT NS 7 FORRRANET IER D Pause Live Video for Video Settings Video Settings
114. sETUP VIDEO CAPTURE STANDARD SAVE ALL DELETE ALL FERRI SE ADHI VIDEO THUMBNAIL GALLERY WDR YJBRONET Windows IJl VIDEO COMPRESSOR codec VIDEO COMPRESSOR 89 ZILOS tk Video Settings codec avi mmv wm Windows Media Encoder 9 Windows Movie Maker ZILOS tk
115. 301254 Zeiss 10 35 405 0 5x C Mount 301250 C 301256 Zeiss Axiovert 25 40 OEM 0 5x C Mount 730064 Zeiss Axiovert 100 135 200 1 0 5x C Mount 301250 va _ SOU at K 301255 2 300936 Lumenera 5 ZILOS tk Chapter 1 Introduction to ZILOS tk ZILOS tk ZILOS tk 5 3 10 CONTROL PANEL 6 9 8 File Setup Video Settings Align Target Help Laser Clinical IR Low Medi
116. 3 BASIC COLORS DEFINE CUSTOM COLORS 4 Ok SAVE EXIT Report ASCII REPORT ASCIL si els 5 vere Images in Report ASCII File Export aser Objectives 2lmages Data File Name Export B Crosshairs n 4 Images File Folder C IVF Data Image Capture Video Capture Date Format ASCII File Import Video Devices Audio Feedback MM DDAYYYY File Name Import mer ol System C DD MM YYYY File Folder C IVF Data YYY MM DD Import Translations Report Saving Options C Report Only Report File Folder Repet gt ASEl ENVF JPEG Fies LA gt I Save Exit Cancel E 6 13 L7R K ASCI OZEE 70 ZILOS tk Chapter 6 The Setup Screens Images in Report LiR KOMAEERLES 1 2IMAGES DATA 4IMAGEs 2 SAVE EXIT 2 Images Data 2
117. Blt FEN LA ORF ANIA PAD COREL 155 ZILOS tk Appendix C Database Communication A B C D El C 5 FRA BORE REPORT IMAGES A B CD BQ C 5 REPORT FILE FOLDER C 2 A B C D Patient ID_Date_Oocyte_Date_Embryo_ID_A JPG 123456789 2005 4 16_2005 5 24_77366_A JPG 123456789_2005_4_16_2005_5_24_77366_B JPG 123456789_2005_4_16_2005_5_24 77366_C UPG 123456789_2005_4 16_2005_5_ 24_77366_D JPG ZILOS tk jpg REPoRT IMAGES REP 123456789_2005_4_16_2005_5_24_77366_REP JPG 156 0
118. BE 8 8 STop 88 E 8 8 REIP ET I I ORREIK RoE RRR RA GREE LE COS 1E e STOP VIDEO THUMBNAIL GALLERY 110 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation REGIA ANTETPEBES SI_IE e PLAY EFA ORI E PFFELE F SIRE s OEM E 8 9 RIO REER S RE OREREY EFA BLE FI FV GEI RS 1 e VIDEO PLAYBACK RESUME LIVE VIDEO RIFLE IF ILERI
119. IMAGE TOOLBOX IMAGE TOOLBOX Image Capture Ay Capture Overlay E Auto Capture on Laser Fire EJ 8 17 RIE ATREZIK HO Image Toolbox IMAGE TOOLBOX E Image Toolbox co Tjoo Thickness BE 8 18 Image Toolbox OI 116 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation e IMAGE TOOLBOX TEXT DRAW ELLIPSE DRAW RECTANGLE DRAW LINE MEASURE DRAWING COLOR THICKNESS UNDO iF BM GEILE MANLP ITAL EFEN CMe RANT IT Tet TEXT 1 TEXT 2
120. 141 P ae SOS DAB SE FL cece cccceccccccssssssssssssseseseseescssesesssssssssnsees 145 23 24 23 CIC tite nese 24 iv Index ZILOS tk 143 nut 145 143 54 SS NN 55
121. 12 15 15 Shot Limit Low 15 15 medium 15 10 HiGH 15 1 15 Cancellation oFF BAIL Low OFF MEDIUM OFF 107 ZILOS
122. BY 4 R 7 C C ZILOS tk C ZILOS tk Chapter 1 Introduction to ZILOS tk OEM 447 C HT C 300950 301149 0 5x Olympus Part U TVO 5XC 2 730064 0 50x X 2 OELMBLID C Leica DMIL amp DMIRB HC Optics Olympus IX 51 71 81 CKX41 BX 3 Zeiss 25 40 ZILOS tk Chapter 1 Introduction to ZILOS tk C No Zeiss 10 35 405 EIRS GI N CORUM CTAKBACI CVI F Ube PESI ET A CO FAbSAH EWG DOLES HT 301250 C 0 50x
123. Label LABEL TEXT LABEL TEXT PROGRAM FIELD LABEL TEXT 9 LABEL TEXT AYDRIFFFA TE 1 PROGRAM FIELDS 2 ADD LABEL TEXT Label Program Fields lt REI gt Report Embryo ID Add lt RPD gt Report Patient Date of Birth a lt RPIF gt Report Partner Info lt REI gt Report Embryo ID lt ROD gt Report Gocyte Retreival Date lt RDC gt Report DSagnosis and comments lt RFM gt Report Fertilization Method lt REPC gt Report Embryos per cycle lt RCN gt Report Cycle Number v 6 34
124. 1 2 ZILOS tk Hide Target M leasurement Toolbox Status Laser Controller is Disconnected it Calibration Factor 0 FEF 5 1 ZILOS tk DF IL 2 LIVE VIDEO WINDOW CONTROL PANEL 2 LIVE VIDEO WINDOW ciRcLE ARRow ISOTHERM RINGS 51 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface 4 coNTRoL PANEL
125. DURATION Class 1 Laser built in Safety Delay None NONE DURATION Class 1 Laser built in Safety Delay NoNE CAPTURE OVERLAY L F EHBOL TYFRROKBEERS ENIE 65 ZILOS tk Chapter 6 The Setup Screens 1 BLANKING DURATION BLACK HIDE NONE 2 SAVE EXIT
126. Version 5 1 ZILOS tk BMP CALIBRATION FACTOR IMAGE DOES NOT CONTAIN CALIBRATION FACTOR CALIBRATION FACTOR B 8 21 Calibration Factor AF S62 XYe LY CALIBRATION FACTOR Ol WGA oT HEFITGERIS A tBOBGECS ELL WEEITI OIL TOB Fr ZF r n EZ ElAL CALIBRATION FACTOR OBJECTIVES OK RAL EI bA ALERTS NIE CALIBRATION FACTOR El CHEILZVFF 120 9 IN Bi ER EKOMENREL CIF A ZILOS tk VALIDATION MODE PULSE DURATION us PULSE DURATION 3000 ps
127. 23 E 3 7 KAL YF CLAN FR TS FAEFS 24 EY 3 8 ZILOS tk 25 E 3 9 QDYDFYbhEL OD YEO CL P EL 1 NERESZ 25 3 10 amp OD USB 26 BY 3 11 12 pin P F 28 E 3 12 29 El 3 13 29 E 3 14 ALE FAL F 30 E 3 15 WY AL P TOEF 97 E 3 16 32 El 3 17 L Y IvA DhE ry eS 33 E 3 18 L P aL haA 3s OFe ERI eS 33 A 4 1 a ZILOS tk ZILOS tk 36 E 4 2 Y
128. 4 1b 1 4 4 2 4 2 35 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface e 4 3 E BJ 4 1 a ZILOS tk ZILOS tk d E 4 2 a ZIA RMS FAT d FLAK e E 4 3 36 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface RED i RED i RED i e RED i e USB s 2 2 BI 4 4 PRED a USB
129. HT 301251 301252 301253 301254 B Clamp F Clamp G Clamp H Clamp 30125 C F Cra ci Eup P Nikon TE200 300 2000 TS100 Diaphot 200 300 Nikon Diaphot Nikon 200 300 2000 TS100 Olympus IX50 IX70 IX51 71 81 CKX41 BX 3 Olympus IMT 2 ZILOS tk Chapter 1 Introduction to ZILOS tk C HT 30125 C K Clamp Zeiss Axiovert 100 135 200 301255 L Clamp Zeiss 10 35 405 301256 V Clamp Leica DMIL DMIRB HC Optics 301257 K 3 C ZILOS tk Chapter 1 Introduction to ZILOS tk C we BOAXSEMAF SERS I LS TED YES Lumenera P
130. view report minimize exit File Setup Video Setting view Report CtrlTR Minimize Exit EJ e FILE VIEW REPORT D4ALEDERIMEF ENIE s FiLE MINIMIZE ZILOS tk ZAJ ZLE TIENI 1 FILE gt EXT 2 ves No Monday August 20 2007 lewoon THORNE BIOSCIENCES 3 41 58 PM Are you sure you want to exit MV Show this message next time No EI 5 5 FODPDLIE TERORY EI 53 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface SHOW THIS MESSAGE NEXT TIME es SETUP SYSTEM PROGRAM EXIT KREOUBIT 1 ND BIE CHOI SL ERTL CLFISAGEEUC LD SHOW THIS MESSAGE NEXT
131. Manual Ask for File Name FAA COMBE FPANGFREAL savE AS Auto Use Report File Name PATIENT ID ID DATE OF OOCYTE RETRIEVAL CURRENT DATE EMBRYO ID ID 87 ZILOS tk Chapter 6 The Setup Screens NE REO LADY SHEE LO TH YERSNET Patient ID Current Date Counter PID 1234 2005_03_17 B_21_2009 EID1234 74jpg Date of Oocyte Retrieval Embryo ID 6 37 Auto Use Report File Name F IRLEGEOIPINEO B PATIENT ID DATE OF OOCYTE RETRIEVAL EMBRYO ID 132 Auto Use Root Name Sz VIDEO FILE NAME ERATE ViDEO FILE NAME count
132. 2 Number DrillM3 Drill 3 Number DrillM4 Drill 4 Number DrillM5 Drill 5 Number DrillMean Drill M1 M5 Number DrillSD Drill M1 M5 Number RulerM1 Ruler 1 Number RulerM2 Ruler 2 Number RulerM3 Ruler 3 Number RulerM4 Ruler 4 Number RulerM5 Ruler 5 Number RulerMean Ruler M1 M5 Number RulerSD Ruler M1 M5 Number Date_Oocyte ddmm yyyy Date CAPTURE_LOCATION Raw Image Text Report_Location Report Text JPGImageA Report Image A Text JPGImageB Report Image B Text JPGImageC Report Image C Text JPGImageD Report Image D Text MER mer MERGE RecDate MERGE mer
133. IMAGES SETUP gt REPORT ASCI IMAGES IN REPORT 2 4 11 3 Images 2 Images Data FFRLEGS 2 IMAGES data 2 REPORT IMAGE 1 REPORT IMAGE 2 4 IMAGES REPORT 4 IMAGES 2 1 2 REPORT IMAGE 3 REPORT IMAGE 4 135 ZILOS tk Chapter 11 Reports ZONA EMBRYO PN1 PN2 DRILL RULER ENTER
134. Nikon Diaphot Models RMS Zeiss Olympus Leitz Fluorovert RMS 2 Leica Leica DMIL 25 Leica DMIRB 18 gt sil 3 Zeiss Zeiss 200 Axiovert 27 4 Nikon Nikon CFI60 optics 25 Eclipse TE 200 300 Eclipse TE 2000 7 4 5 nore LRILIN FIZIER NCL S LOD YEREL CAL TCE Sb 39 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface E 4 7 RAL LF CLAN PIA SI FAETS 6 EJ 4 8 ZILOS tk 7 noe L Y E1 JL 4 AXIO GUA DAM ELIRI NCL OD YERE SATUGI NILGYF LA L P EL 1 Nh A OY L Y ORSNEOTDCLEWEF SO SERB OL RIL NAG RAL SIT BAIT EREB CA
135. ZILOS tk Chapter 6 The Setup Screens Save in jo IVF Data 2 Import mer G E 6 19 ASCII 2 FILE FOLDER BROWSE FiLE NAME 3 SAVE EXIT ASCI FILE IMPORT Import Translations IMPORT TRANSLATIONS ZILOS tk ZILOS tk iMPoRT NAME YoB YEAR OF BIRTH ZILOS tk DATE OF BIRTH YOB Import Translations ZILOS tk Report Fields Patient ID Patient Name Embryo ID Date Of Birth Oocyte Retrieval Date EJ 6 20 Import Transl
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138. Devices Setup Laser Objectives WARNING Crosshairs Changing the video device video resolution or video Report ASCIl display type will require recalibrating the magnification for Image Capture all objective lenses and realigning the laser target Video Capture Video Devices Video Devices Audio Feedback 1CRLoosightCamers Q r Pause Live Video System CRI Oosight Camera for Video Settings Video Resolutions Change Video Display Type Fill Video Area C Keep Original Size Video Frame Rate Camera Input Screen Output v WY 487 IV Always Attempt Input Save Exit Cancel D 11 Video Device DR BE Oosight Camera 2 VIDEO RESOLUTION CHANGE VIDEO CAPTURE PIN 167 ZILOS tk Appendix D Video Settings amp Resolutions NOTE Video Capture Pin Stream Format Pixel format 8 bits Bayer Output size 1392x1040 Frame rate 14 79 fps BY D 12 Video Capture Pin e PIXEL FORMAT 8 BITS BAYER e OUTPUT SIZE 1392x1040 e FRAME RATE 14 16 fps es OK Video Device VIDEO D
139. Marks A LotS 5 Adjustment Screw 6 175 ZILOS tk Appendix F Laser Confocality 2 8 mm Laser Adjustment Screw Laser Adjustment Screw 5 2 F 1
140. es THICKNESS iz Thickness EX FILISZBALELA Undo UNDO e UNDO RRA LEELF ILERIK 1 BROWSE OPEN IMAGE 119 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation Open Image Look in Capture et Fe ImagecalTest0 jpq test jpg gt E RMAImage1 jpg My Recent e RMAImage2 jpg Documents RMAImage3 jpg RMAImage4 jpg RMAImageS jpg RMAImage6 jpg E RMAImage8 jpg E RMAImage9 jpg E RMAImage10 jpg E RMAImage11 jpg E RMAImage12 jpg 5 RMAImage13 jpg RMAImage14 jpg E RMAImage15 jpg My Network File name bd Places Files of type Images X Cancel J 8 20 Open Image 2 OPEN
141. 82 ZILOS tk Chapter 6 The Setup Screens Auto Labels AUTO LABELS TIME DATE OBJECTIVE INFORMATION EMBRYO ID PATIENT ID e SETUP IMAGE CAPTURE AUTO LABELS E Setup File Storage Auto Labels Laser Auto Label Name Objectives Crosshairs we Center lt Edit Delete Report A4SCIl _New a Delete nage Capture Location On Image gt Test Color 4 Enabled Video Capture Edit Label Locations Vv Video Devices Fort Audio Feedback System Name
142. 90 ZILOS tk Chapter 6 The Setup Screens Time Lapse TIME LAPSE a El Setup Standard Time Lapse Laser Objectives Crosshairs Days Hours Minutes Seconds Report ASCII E Image Capture M Capture Interval m Capture Duration Video Devices Audio Feedback 0 No Limit System Days Hours Minutes Seconds 0 0 p 0 Playback Speed Frames Per Second oy Save Exit Cancel EY 6 41 Time Lapse ORE HH Capture Interval Capture Duration Playback Speed PLAYBACK SPEED 1 91 ZILOS tk Chapter 6 The Setup Screens
143. e PRONUCLEI 1 e PRONUCLEI 2 e DRILL e RULER 1 2 TooL TYPE NOTES MEASUREMENT TOOLBOX IZRI COATES E ED CEET 7 Measurement Toolbox Drill Ruler Pronucleus 1 Pronucleus 2 EV 0 1 Measurement Toolbox Tool Type 126 ZILOS tk Chapter 10 Documentation 1 2 3 9 MEASUREMENT TOOLBOX TOOL TYPE START A
144. 3 6 1 6 2 FEHFSL Y ODNNVAWEERYS ENIE e LOW MEDIUM HIGH 2 OFF 8 3 HiGH Low MED OFF 106 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation Laser Clinical Low Medium TE Hide Target E 8 3 HIGH TRE Timer Bar ON OFF 15 8 3
145. 6 28 Browse For Folder 3 ok IMAGE FILE FOLDER 4 SAVE EXIT Image File Name Label IMAGE FILE NAME LABEL ENABLED 2 FILE NAME ONLY FULL PATH FILE NAME ge File Name Label IV Enabled Full Path File Name lt File Name Only OS Color Chart Palette 6 29 BIF ILONI DA NEEF ASKS BILE e ENABLED FULL PATH FILE NAME FILE NAME ONLY TIIR ED BEGIRA IE e FULLPATH FILE NAME
146. 19 VE SO TE ET E E E TES A A E E E E EST 19 20 f EE ABRS ZILOS th A E SELAA AE 1E 21 27 22 ee 22 FEE OMB A 22 RI 22 LPP ELAN RR 23 SRR eee Te LORE AERP TSO nT ELE PN MOTTE ree Rete eee aera ee eT Mere RT re 26 VET E OE ia T 32 EAI IF OWN DE AOO EAA EENAA 32 TDD A IEA IE ET EENET A E EA A E E E E 35 35 ED 37 f EE ABAS ZILOS th A E ELARRE 1E 37 37 EF OMA IT a aba etd ts ec ii ws ete n 37 lt 38 ET DE ee a E ee Len eee CC Een A eee eee en ete Pee 41 46 PPR ASTRO RLY TTT vis cov Baise kilts ci seh aes RRR Rh RES 46 ADAD A 51 TIBI J PIN AI NN Ni 52 Pile ARI NARE PR 53 Seti a DAAE A N A EA A E EA A E E 54 Video Settings 54 Objectives XPIQLALL R a 54 ZOO a a Bt cer be esc att be cae aees Dea as tA hes athe esas N E etal he 55 ZILOS tk X of Contents Target a aa 56 A A A 5 PPP 57 4S 59 Remote Fire a ia a 1 60 Com Port m AN ia 60 696 67 E EAI A FOES 61 areet 62 Blanking Duration 65 SU ENE kk lt 66 Energy Constant 66 66 Objective Calibrati
147. E 4 15 APL a GOVE bh IYI RAIF 46 E 4 16 ZILOS tk FYThYIOT IN EKG 47 EY 4 17 ZILOS tk DUP KY AOR FVII EEFJIX IEJ FL IAL BOVE KIYBR AY FERAL a 47 EY 4 18 ZILOS tk FYAhwAIOKR FFODHETHLADSIER VIV GOVE KIY KAA YF EA LEGGE 48 Bl 4 19 L P aL haA 3s OXKM YES 49 E 4 20 L P aL hA 3s OfE XY eS 49 BY 5 1 ZILOS tk DE LP 51 5 2 Clinical Clinical E KFFOIALY KOA NANRAE 52 Bl 5 3 Af X 1 OVWE 53 EY 5 4 53 E 5 5 53 E 5 6 HOLY ROPGNA OY XZ1 54 EI 5 7 33 EW 5 8 Pan Image 955 BE 5 9 56 5 10 RED IL 1 NEARAL TUS GR OL F yf ALA 56 EY 5 11 I RED l DH ERE 7 57 6 1 Laser DREH Clinical Clinical E F 59 6 2 NN AWGOFTIA LME 61 E 6 3 3 PYbRROEE 62 El 6 4 Y 2PNEKHOZ TIRA 63 E 6 5 1 FRU IRR FCN CTOAAVTRRIKEILRESN TOBHA 63 E 6 6 FRRVULIORT HRT 64 E 6 Z FEEOFRRYLOFISVERRSES EA 50 COVYIODARR H 100 ARR C 64 ZILOS tk List of X s E 6 8 DFFA TRAORE 65 E 6 9 Stb R ORE B E 67 E 6 10 POKASRILDSOREA E 68
148. E 6 11 69 BL 6 12 70 E 6 13 4S 7 OR EAH 70 6 14 2 Image Data I 71 E 6 15 4 G G HRAOLA K HEFT 2LLL 72 EJ 6 16 Aftf7 VYbKOEFR 73 6 17 ASCI 73 6 18 ASCII 74 6 19 ASCI 75 El 6 20 73 E 6 21 LR FORFA E 76 6 22 File Storage ORE AH 77 E 6 23 BIP ILORE HAR 78 6 24 Auto Use Report File Name HEIRLEGROIPINEO B 78 E 6 25 BIr ILO RE HRF 79 J 6 26 Auto Use Report File Name F RLIEGEOIrTILEO b 80 E 6 27 EJIP L IL IF NA ORE 80 6 28 Browse For Folder 81 6 29 EJIP ILOZNIL 81 6 30 82 EY 6 31 BFBML 82 6 32 Auto Lables DZE EIH 83 E 6 33 84 EY 6 34 Program Fields 85 BY 6 35 LABEL TEXT 86 E 6 36 EFA FPA FV OBRARE 87 6 37 Auto Use Report File Name F RLIEGEOIrPTILEO b 88 B 6 38 EFT AL IL YY ORERE 89 E
149. EMI 50 COVYIOARR AAI COYVYIODARK 64 ZILOS tk Chapter 6 The Setup Screens MRVULIERR HERR ENIE 1 2 SAVE EXIT Blanking Duration Blanking Duration Duration Black D Sec e Hide C None E 6 8 FFAEL TORE Hide HIDE DURATION Class 1 Laser built in Safety Delay Black BLACK
150. Image Properties 1 EXPOSURE 67 2 GAIN 1 000 3 RED GAIN 1 36 4 GREEN 1 GAIN 1 5 EQUAL TO GREEN 2 GAIN 5 GREEN 2 GAIN 1 5 EQUAL TO GREEN 1 GAIN 6 BLUE GAIN 2 5 X 12 Image Properties D MRE IE NE BERS SEO EMRE IAI ORME SF SECKEILBUF CAM AUTO exposure Di HILBEHO CZFUA A AI NFLARAEHESFS AUTO WHITE BALANCE BREO EHVLPHOLFT 4 VIDEO CAPTURE FILTER IMAGE PROPERTIES 2 169 ZILOS tk Appendix D Video Settings amp Resolutions Video Capture Filter Initialize Reset Gamma 00 Contrast 10 00 Brightness if D 14 Video Capture Filter Image Properties 5 13 IMAGE PROPERTIES 2 Image Properties 2 1 GAMMA 1 00 2 CONTRAST 0 3 BRIGHTNESS 0 X 13 Image Properties 2 O2EMZRE Ee NOTE CAMERA CONTROL VIDEO PROC AMP SIDR EILE OFF 170 E ZILOS tk
151. 1 15 pin D USB 2 2 USB 2 USB 3 3 USB 3 USB iF USB USB RKO EYGUBE WALET BY 3 16 32 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 1 2 3 4 ON
152. 1 BROWSE 8 7 2 3 OPEN Video Slider VIDEO SLIDER E 8 10 e VIDEO SLIDER F HERI EFL TAEZ TABEL TEAL 111 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation VIDEO THUMBNAIL GALLERY vipEo DISPLAY VIDEO icon Video Icon Images Video Display Button gt Videos BT 8 11 YLRINEERL ETHEINGARCHSINE e VIDEO THUMBNAIL e SCROLL BAR
153. IMAGE CAPTURE FILE STORAGE AUTO LABELS 2 File Storage Setup Laser Objectives Crosshairs Report ASCIl Video Capture Video Devices Audio Feedback System nore JPG FLE S TIF Ft CRHLEE File Storage Auto Labels Image Type JPG Quality BMP JPG TIFF Minimum Quality Individual Image File Storage Manual Ask for file name C Auto Use Report file name Auto Use Root name Root Name Count SinglelmageRoot 8 Reset Still Image Location fico Maximum Quality Multiple Image Storage Save All C Manual Ask for file name C Auto Use Report file name Auto Use Root name Root Name Count MultilmageRoot 10 Reset ImageFileFolder Ccapue Image File Name Label ge Full Path M Enabled File Name File Name Only Watermark E V Enabled Save Exit Cancel BY 6 22 File Storage ORBEA A Image Type e BMP TIFF Jpc 80 6 5S OF calibration factor LET PINDAYRNBEUENES RALLI IL AARAA CHEEITIVEWGEAIL BMP BHAtEERLECCRKAS JPG Quality J
154. ZILOS tk Appendix F Laser Confocality F 1 0 100 300 us Laser Adjustment Screw 9 Laser Adjustment Screw 45 Laser Adjustment Screw 9 Laser Adjustment Screw 45 F 1 177 G USB 720421 USB LASER CONTROLLER 1 7
155. A OCES BEARBOELEASL MIDOEEEBU KA ILBIKF lt INE AISA BASE EILA 148 B Version 5 1 ZILOS tk JPG TIF JPG TIF ARG LAIN FHFYNRRSN TU SIRY iz BMP fezt CRANE EZF ILON YS ILlk FRUIL L 37 RS VIFRILEPANECAD BMP Hit Ce FAL BAAN lt BMP SAE DRGFHAEL TILEY CHITA JPG TIF CAPTURE IMAGE CALIBRATION FACTOR
156. IMPORT FILE IMPORT FILE Asc FILE IMPORT 74 PATIENT ID NAME EMBRYO ID DATE OF BIRTH OOCYTE RETRIEVAL DATE 5 Patient ID ID Number Name Text Embryo_ID SNH AG AE PN EHIK REO ID Number YOB dd mm yyyy Date Date_Oocyte ddmm yyyy Date 137 ZILOS tk Chapter 11 Reports Clear Export NOTE CLEAR EXPORT KEREILA Dv al OK CHAOR TAIL TITOR 7 DAI RRENEAN BUET CLEAR EXPORT FHEEL TU tA WR PPILNOF AHBELEBES EF AR KYFIKECRHAOGES DA lt OEREFRIEL KESL CRLIKOGHC
157. Laser Controller is Disconnected 3 18 D P qy O 3 OFfE MY eS 33 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 1 FILE EXIT ZILOS tk 2 ZILOS tk Windows Task Bar START 3 sHuT DowN 4 Windows SHUT DOWN WINDOWS SHUT DOWN 5 NVALYOBERODNSEE TCL RADDYEF Windows EL lt woe BRITT TIKES SRAM RESGOF_Y AY FILA STEAAR T Itata CLIE 34 4 Bata ZILOS tk
158. Program Fields LABEL TEXT 3 85 ZILOS tk Chapter 6 The Setup Screens Label Program Fields lt Rop gt Report Oocyte Retreival Date Add Label Text BY 6 35 LABEL TEXT LABEL TEXT 4 SAVE EXIT lt T gt lt D gt lt OBJN gt lt OBJC gt lt RPN gt lt RPID gt lt RPD gt lt RPIF gt lt RED lt ROD gt lt RDC gt lt RFM gt lt REPC gt lt RCN gt AS Time Date Objective Name Objective Calibration Factor Report Patient Name Report Patient ID ID Report Patient Date of Birth 7 Report Partner Info Report Embryo ID 7 AB ID Report Oocyte Retrieval Date 7 Report Diagnosis and Comments Report Ferti
159. Size Arial fi 2 B 7 U Change Label Program Fields lt T gt Time X Add Label Text IVF Center Patient Name lt RPN gt Patient ID lt RPID gt Embryo ID lt REI gt lt T gt lt D gt Save Exit Cancel EY 6 32 Auto Lables OBZ IZI Auto Label Name 1 NEw IVF CENTER o 2 ok AUTO LABEL NAME 83 ZILOS tk Chapter 6 The Setup Screens 1 EDIT 2 ok AUTO LABEL NAME PANE EAUIRI BIKE 1 AUTO LABEL NAME 2 DELETE AUTo LABEL NAME Location on Image
160. 141 47 103 A A AEAN 55 103 i 120 131 149 010D NN 3 3 27 42 88 113 91 88 113 CCD 3 22 37 clear export 136 Clinical 106 Clinical 52 99 C 4 C 9 or 171 n a 69 60 UCB TFT 60 1 17 52 LE 2 12 34 26 41 42 107 134 63 lt 62 63 63 aed nnnnnnnnnnnnnnnnnnn 134 drill 129 137 mer 154 ASCI 155 152 ield headef
161. 157 ZILOS tk Appendix D Video Settings amp Resolutions Video Settings VIDEO SETTINGS Generic WDM Filter Proxy Generic WDM Filter Proxy Video Decoder Video Image Video Decoder Video Prac Amp Video Image Brightness fize E Image Mask Fy Mask noisy signals at top and bottom ee ee a Flip Vertical Saturation 4 32 ja Flip Horizontal Sharpness p pa Audio Path oa ja eono jm Default Auto Cancel Apply Cancel Apply D 2 Video Settings OBREAH TIFI FRERET 1 VIDEO SETTINGS 2 VIDEO PROC AMP tab DEFAULT 3 OK Lumenera Camera Video Device 1 SETUP VIDEO DEVICES VIDEOo DEVICES LUMENERA USB 2 0 CAMERA 158 ZILOS tk Appendix D Video Settings amp Resolutions cl ET Setup thee WARNING OS Changing the video device video resolution or video
162. Changing the video compressor may result in program failure and video codec incompatibilities LEAD MCMP MJPEG Codec Sr 6 39 Advanced KE ERZA codec DIO NF EEE ST ENI e VIDEO COMPRESSOR PROPERTIES VIDEO COMPRESSOR codec 89 ZILOS tk Chapter 6 The Setup Screens Video Compressor Codec Properties About Encoder settings Flip YUY data Format JPEG 4 1 1 X Quality Better Quality Smaller Size mese Nee o Decoder settings V Enable E 6 40 BRIJIT CHEDRGETAEUERS S 1k 1 VIDEO COMPRESSOR CODEC PROPERTIES 2 FORMAT JPEG 4 4 4 JPEG 4 2 2 JPEG 4 1 1 JPEG 4 1 1 CF 3 QUALITY 1 255 30 4 INTERLEAVE NEVER 5 DECODER SETTINGS ENABLE 6 ok
163. Elt BEIT EVE TC ZILOS th DERI FORRES EVE CES AI BAT EETA IT EEBB OLET 18 3 ZILOS tk 3 es RED i RED i USB 9 pin D 9 pin D 12 pin USB 2 0 2
164. FILL VIDEO DISPLAY s 1600x1200 KEEP ORIGINAL SIZE VIDEO FRAME RATE ALWAYS ATTEMPT INPUT SAVE EXIT video settings OREM RI ILAN SF CZ VIDEO FRAME RATE 7 RAMORRANGZWICEMBVFEIF COGS video settings exposure FF RIARI 67 LUF LE LAARI S6LEMDVFF Video Settings 1 2 ViDEO SETTINGS VIDEO CAPTURE FILTER IMAGE PROPERTIES tab exposure auto AUTO WHITE BALANCE Generic WDM Filter Proxy Image properties Image properties 2 Camera Control Video Proc Amp Exposure 66 5 ms Auo Gain 1 000 Auto Fied gain j 2000 Green 1 gain 500 Auto white Green 2 gain J 1 500 balance Blue gain j 2500 Cancel Apply D 5 Video Capture Filter Image Properties 160 ZILOS tk Appendix D Video Settings amp Resolutions 3 10 IMAGE PROPERTIES
165. Laser Clinical BE Medium High Hide Target Measurement Toolbox Tool Tore E coe so oo Video Record and Playback IE Time Lapse Mode CIO Image Capture i Capture Overlay a Auto Capture on Laser Fire rl Ep EJ 8 1 105 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation Low MED HIGH oFF Laser Clinical Low Medium High BY 8 2 Laser DAPAKBOIYKA N NAW Low MEDIUM HIGH 3
166. OFF Windows 5 ZILOS tk ZILOS tk ZILOS tk O EPIL 6 STATUS BAR 1 5 LASER CONTROLLER CONNECTED ON COM PORT 1 Status Laser Controller Connected on Com Port 1 Bl 3 17 LASER CONTROLLER IS DISCONNECTED 21 HO LED Status
167. TIME KEIER S lt FLYIV IEAN Setup SETUP Video Settings VIDEO SETTINGS Objectives OBJECTIVES BHI F UB SHYLY KEEL TLIC L Y CBB BE BLIER OEE ES LEIEFI CY acetal o Align Targe 1 ZILOS tk 2 Low Mag 4x 3 RD 0024 No Name Set No Name Set No Name Set oun e E 5 6 SMYLYROING DS XZ1 SLY AER ENI s
168. of Seo Cee ee OE eT eee 89 EE EaI DA E 103 89 60 SR 90 96 L114JE 97 Oosighk LIAR 167 oirr 97 Video Devices 92 56 ye 92 62 93 SS 63 Fill Video Are 93 SEO 63 Keep Original Size 93 63 88 117 16 Always Attempt Input 94 115 Camera Input ccesscssssssscssssesscssssesscsssseessssssesscsssseescessseess 94 115 Screen Output 94 n a DE 116 93 a ae OO oe aoe 115 Fs 115 eae oe certian ee eeu rent 158 112 Lumenera 160 112 Lumenera 162 III 112 Oosight LL 167 112 A Video SOttinSs ngain NS 54 91 111 91 113 goes EA RR EP 91 107 ANAE
169. tk Chapter 8 The Clinical Control Panel and System Operation NOTE LHEIT HOFI AAW EL SANE CLER LTE Z 7 EESE a E 8 4 VL R ROARS E EFRE TEL KORA RSS A L F ERHS ENI e NE bH ELE S 15 OIE AN CUED TORI HC FSH FRLENLAIB ko CHEYES AE OAIR HIDE TARGET R PY RET IKE e HIDE TARGET B PIbRREIHFRENIIF BE LYA D I I II KEE LEY HERS BLEW CESKY ETF B P YIR RANER TINI e SHOW TARGET
170. 0 2 Nikon TE 2000 TS 100 0 5x C Mount F 0 5x C Mount B 1 0 5x C Mount B 2 Lumenera 0 5x C Mount B 301250 301252 301250 301251 301250 301251 300788 301250 301251 Olympus IX50 70 2 1 0 5x C Mount G 2 Lumenera 301250 301253 730063 ZILOS tk Chapter 1 Introduction to ZILOS tk C Olympus IX51 71 81 1 OEM 0 5x C Mount 301149 CKX41 BX 3 2 0 5x C Mount 301250 gt 3 PODARI Aa G 301253 Olympus IMT 2 0 5x C Mount 301250 H
171. 089 1512 T3 GlobTek GT 210097 50121 amp E N BEL TEAL 1 FPHOFHETADSINOBRHABIL 45 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface FIRE BuTToN REMOTE FOOT SWwITCH e 1 s USB 2 e USB 3 1 1 USB 2 2 USB 2 USB 3 3 USB 3 USB BY 4 15 USB K KMGUIGER USB USB IIZDLIFEKEILARECI DO EF
172. 116 E 8 19 KHATER AE 118 EV 8 20 Open Image 120 E 8 21 YLRANVLIKRRFASNE S Calibration Factor LEAF 6 ZB XYeC LY 120 9 1 Laser DREH Validation Mode 121 B 9 2 BEIEE KROL Y LALF LV AA IL NAIL 122 EY 10 1 Measurement Toolbox Tool Type 126 10 2 Zona LEH EK 128 10 3 Embryo Y LEH EK 128 El 10 4 WEH 129 10 5 Drill Y LEH ERI 129 E 10 6 Ruler Y LEBMEW COBA HROVIAKHE 130 10 7 Open Image 130 ET 10 8 HAPLILEIR TRENS Calibration Factor LEAF 6 ZEY E gt 131 11 1 133 E 11 2 134 11 3 Images 2 Images Data FERLEGSA 135 E 11 4 MEFO EZI 136 11 5 Import MER Fields 139 E 12 1 EVID OER EWARILANEI TIREOB OT SKE 143 ER 7 C Ad i ed E 4 0 5 R 3 eee ee eee 7 R 4 C nnrir 8 eT Fa ei i ede eee 10 T O 00 es 24 0000 39 R 8 ee sree esos 64 Fe EZO A AAO ee reson eee oO 86 FE 10 Image Properties gt 161 X 11 Image Pr
173. 2 10a 10b a 2 Laser Adjustment Screw 5 Laser Adjustment Screw b 2 Laser Adjustment Screw 5 Laser Adjustment Screw a 0 Optimum Confocality Ww Counter Clockwise EY F 1 L P D4 ARORE 176
174. 2 305 0 14 356 0 16 406 14 172 ZILOS tk Appendix E Using Shim Spacers BWA IE 450 1 0 16 0 002 406u 51u 457p 2 0 014 0 004 356u 102h 458p 3 0 012 0 006 305u 152h 457p 4 0 010 0 008 254u 203u 457p 450 lt 50 um 173 LASER CONFOCALITY
175. AH 141 108 O R nE AE E EE 108 ae 39 142 40 13 32 46 96 66 36 io 102 HIGH JE 22 37 1 143 55 Image Capture 114 66 139 107 140 175 Image Capture 77 141 ate errata 83 141 IPANDE asso N IR 83 26 41 113 175 120 131 149 175 81 2 12 EDES See erate e ee ere 81 142 3 81 118 by 81 77 20 36 auto report file name ee 78 1 auto
176. BER ZIE 1 0x OXJFBALEGEIZ PYDL LaLERBUTITIQDEMDVYET 1 100 2 100 2 3 SETUP OBJECTIVES 4 BEGIN CALIBRATION 2 7 3 tatus Laser Controller Connected on Com Port 1 BE 7 3 F 4rVFIL S sae 5 100 103 ZILOS tk Chapter 7 Target Alignment and Magnification Calibration CALIBRATION OK SETUP OBJECTIVES CALIBRATION FACTOR
177. CELG AG HAO F Finl LIVE VIDEO 2 AMI lt LHFSN TOBERA 12E CILBYVF LAD CITB LIVE VIDEO RIVET E FAGONEX BSIKRRANFT 114 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation IMAGE THUMBNAIL GALLERY IMAGE DISPLAY IMAGE ICON Image Icon Image Display Button Ep Images N Videos 8 15 FAL AAG LFIN FrFV YVLRINEERL FELL REALE CASINI s RI LAIN FrFV FAIO N FENI e SCROLL BAR 1IDELFILS SW COMGYLIIWE N KBFIRZD IRFFG BILE 1 Delete Image Delete All Images Save Image Save All Images Print Image Print Thumbnail Images E 8 16 2 save IMAcE
178. DEO AREA FILL VIDEO AREA REES ATT x E 1 FILL VIDEO AREA 2 SAVE EXIT e KEEP ORIGINAL SIZE noe 7 CEN CHS O HN IL KEEP ORIGINAL SIZE COMAEBAOLET wore LUMENERA DIGITAL CAMERA DHA WINDOWS DISPLAY g 1600x1200 KDEEI FILL VIDEO DISPLAY TOAZ 1600x1200 fd FRILENMULDEEIZ KEEP ORIGINAL SIZE COMBERBAOLET 93 ZILOS tk Chapter 6 The Setup Screens wore Cohu GrabbeeX 1 9L TUBA WINDOWS DISPLAY OED 1024x768 LLE CHAIF KEEP ORIGINAL SIZE COLEHFRBHOLET Video Frame Rate e CAMERA INPUT
179. DORE NEFRON RIENI 1 2 3 SAVE IMAGE 127 ZILOS tk Chapter 10 Documentation SETUP IMAGE CAPTURE INDIVIDUAL IMAGE FILE STORAGE ZONA MEASUREMENT TOOL 5 A e 4 12 3 6 9 Measurement Toolbox Tool Type pes A D 1306 Enter E 1111 So 6336 iB EV 0 2 Zona NL EM EK FRO EERIE Y IL EMBRYO MEASUREMENT TOOL SAEOBEeAOSEOOY ILCIF di d2 2 MEAN sp Measurement Toolbox Tool Type Start Report Mean 0 00 SD Blastomeres fo EZ 0 3 Embr
180. Desktop Bl 3 38 BELL KANS Y A ZILOS tk RED i RED i RED i e RED i e USB s 2 2 i ZILOS tk 270448 BU 3 4 RED a2 USB KALY FETC 21 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop ZILOS tk ZILOS tk e 19
181. E AS Auto Use Report File Name EIRDZ L R KD71 LFE F SERASTS PATIENT ID ID DATE OF OOCYTE RETRIEVAL CURRENT DATE EMBRYO D F ID KEOBSILA DL SELL CAYREBRED 79 ZILOS tk Chapter 6 The Setup Screens Patient ID Current Date Counter PID 1234 2005_03_17 B_21_2009 EID1234 7jpg Date of Oocyte Retrieval Embryo ID 6 26 Auto Use Report File Name F1 1 RLEGEOIPINEO B PATIENT ID DATE OF OOCYTE RETRIEVAL EMBRYO ID 132 Auto Use Root Name zt Root Name ERATE ROOT NAME count ROOT NAME
182. E SES SS I 123 Report Image 2 125 Report Image 4 125 HEV OND NE EE 127 PTRO RETREAT HRB O BE RIPT Ceo oecccsccscesscssesssssssssesssssssesusssssssssssssscsussssssuessessesssessecsssseesuessesses 127 728 k 128 2 20 729 KOME Z SNE EN Sopher cbs anal tac Moog RS NN het cl 129 Re et TT hr RR rt 130 ERIFLFETTIB APA NEMEC CHE ENT Dn essccsccsvesseessesssssssssessssssssssssssssssssssussssssssusssessessseesucsuessesaseesecs 130 134 FETE SAME Be SNOEI T Ai ii OA NOR AOE E NTC AE ra PE ATE SOME RTS Ve ere A 135 ERO LG FI EE Sven sx aE kl 136 a 136 ZILOS tk i 137 DEEL DG FEN A R NS ER EN 143 PT FEE E EEEE EEEE 144 O E OI S TE 151 152 EE i 0S 152 Field Headers a 152 154 PP HFP 154 Video Device 157 Video Settings D ER GA R A 1 1 158 Video Device I 158 Wideo Settings Bee ves EN SR YN Rh EE ain he hc a at 160 Lumenera Camera DWI NS 162 Lumenera Camera D1 IFJ Y THERE ccccccccccccscssstsvevscscscscscsvevececscsenenevevsvsvsvavsssvavavscscscsesesesavavavacsenenenes 165 Video evce I 167 ec56 gs 168
183. E TE E S Embryo ID E 6 15 4 AGRAOLA Kh WET ZAL 72 ZILOS tk Chapter 6 The Setup Screens Date Format 3 MM DD YYYY DD MM YYYY YYYY MM DD m Date Format C MM DDAYYY C DD MM YYYY YYYY MM DD E 6 16 1 2 SAVE EXIT ASCI ASCII FILE EXPORT PATIENT RECORD ASCI ASCII File Export File Name Exportmer Ea File Folder IC wF Data EJ 6 17 ASCH 73 ZILOS tk Chapter 6 The Setup Screens DRANELIAWR b aA REI lt TENI 1 FILE NAME
184. H F ENTIE e AUDIO FEEDBACK ENABLED SYSTEM 96 ZILOS tk Chapter 6 The Setup Screens Setup Laser MessageBoxes 0 bjectives IMAGE_HAS_NO_HEADER Crosshairs IMAGE_TOO_LARGE_FOR_VIEWING_AREA Report ASCII PROGRAM_EXIT Image Capture Video Capture Video Devices Hard Drive Free Space Audio Feedback A Dive C i Total Space 74667 MB Free Space 29598 MB Used Space 45069 MB EY 6 44 System OXEHA HA Message Boxes MESSAGE BOXES TNE 1 2
185. HAL VY FEY Hc ZILOS tk ZILOS tk ZILOS tk e 35 1 37 ZILOS tk Chapter 5 Overview of the ZILOS tk Software Interface 2 C 2 3 R 4 3 C 4 5 4
186. I 6 39 Advanced KE 89 E 6 40 90 EZ 6 41 Time Lapse ORE AA 91 EY 6 42 Video Devices ORE HH 92 EZ 6 43 Audio Feedback OREHH 95 EV 6 44 System OHEHA 97 SY 7 1 HIGH RE CL V FHA 100 E 7 2 L P EG ko TAID RELIER YA 100 ZILOS tk List of ls Bl 7 3 r PL Sav ee 103 8 1 Clinical KF O 105 E 8 2 Laser DJ PRKBOALY KON NAIL 106 BE 8 3 HIGH TE 107 E 8 4 VV RROLBRSS Z ERE TECK EO 108 EF 8 5 109 El 8 6 EF FRBERBLOIALY IAN NRW 109 E 8 7 EFA ODRIERSY 110 E 8 8 EREI PER I Hl RRS EHF PILKEY 110 E 8 9 ERIO FFIFILERS K DEREI 111 E 8 10 777 E 8 11 77 E 8 12 112 E 8 13 FELL GARI FP YN 113 EY 8 14 Report Image 114 E 8 15 FL AAV LAIN Fray 115 E 8 16 FRFLAINOX 1 115 BY 8 17 RLF AT REZKO Image Toolbox 116 J 8 18 Image Toolbox
187. I BE Text Cycle Number IVF Number Total Embryos_Cycle Number JPEG_Location REPORT JPEG File Name Prefix Text BT_Coord N A AT_Coord N A ZonaM1 Zona 1 Number ZonaM2 Zona 2 Number ZonaM3 Zona 3 Number ZonaM4 Zona 4 Number ZonaM5 Zona 5 Number ZonaMean Zona M1 M5 Number ZonaSD Zona M1 M5 Number EmbryoM1 Embryo 1 Number EmbryoM2 Embryo 2 Number EmbryoM3 Embryo 3 Number EmbryoM4 Embryo 4 Number EmbryoM5 Embryo 5 Number EmbryoMean Embryo M1 M5 Number EmbryoSD Embryo M1 M5 Number PNd1A Pronucleus A 1 Number PNd2A Pronucleus A 2 Number PNd1B Pronucleus B 1 Number PNd2B Pronucleus B 2 Number PNMeanA Pronucleus A Number PNMeanB Pronucleus B Number DrillM1 Drill 1 Number 153 ZILOS tk Appendix C Database Communication DrillM2 Drill
188. IRB 3 Zeiss Zeiss 200 Axiovert 27 4 Nikon Nikon CFI60 Optics 25 Eclipse TE 200 300 Eclipse TE 2000 6 4 5 iF LIRIWIN F T I II ELIRI NCL SP LOD YEREL CAL TE Sb E 3 7 RALLY FCLARILN FF SFI FAEFS 6 24 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 3 8 ZILOS tk 7 nore HKD CU ASMERI NZL oD VERE 7 L P ELAN BGUIEY L Y ORAUCEDTESTLEEST OH BUBOLAKILNE RAL SIT BEITER BYE HA
189. ISPLAY TYPE FILL VIDEO AREA VIDEO FRAME RATE ALWAYS ATTEMPT INPUT SAVE EXIT video settings OBE Die LAN SF CIF VIDEO FRAME RATE CRAD RRENA ED BYE COB video settings exposure Pea REE 67 UPOLERB DLL L hE EES OLEWBVEFS Video Settings 1 2 VIDEO SETTINGS VIDEO CAPTURE FILTER IMAGE PROPERTIES exposure auto AUTO WHITE BALANCE 168 ZILOS tk Appendix D Video Settings amp Resolutions Generic WDM Filter Proxy Image properties Image properties 2 Camera Control Video Proc Amp Exposure 66 5 ms FF Auto Gain 0 oo Red gain 2 000 Green1gain 1 500 Auto white Green 2 gain H 1 500 balance Blue gain a ee 12 500 Cancel Apply D 13 Video Capture Filter Image Properties 3 12 IMAGE PROPERTIES
190. Il Image Capture Objective Description Video Capture LO tk Begin Calibration Video Devices Audio Feedback System Objective Information Objective Calibration Save Exit Cancel Bl 6 9 HOLLY R ORE EA Objective Calibration MAGNIFICATION CALIBRATION FACTOR BEcIN CALIBRATION MAGNIFICATION CALIBRATION 102 Jog ZILOS tk CALIBRATION FACTOR 67 ZILOS tk Chapter 6 The Setup Screens noe ERY AL Sag ERRI BMP Het CR ANEAEG IT ILII i BAUENF UA BOUMECHPYIL L aT FR AWELLSBAIA BMP HIE CHGERFLEZUEDIICL TZE Crosshairs CROSSHAIRS
191. KME CHAT pPRIiNT SAVE CLEAR jpg wore 2IMAGE DATA 4IMAGES RUIDOIF VYbCIULIEFOALER LYC SETUP gt REPORT ASCH D H 5 74 VYb OB BIT Hr LOZ 7 REPORT PHO SiABANFT SAVE CLEAR SETUP REPORT ASCI 76 REPoRT REPORT ascet ASCII SETUP REPORT ASCII ASCI SETUP 80 THESSA txt IEE mer BACH FANFT NEE IPG fest CLE FANES
192. OPEN IMAGE Open Image Look in E Capture gt e cf B p ImageCalTest0 jpg test jpg RMAImagel jpg My Recent RMAImage2 jpa Documents RMAImage3 jpg Ej RMAImage4 jpg RMAImage5 jpg Desktop RMAImage6 jpg RMAImage8 jpg E RMAImage9 jpg RMAImage10 jpg RMAImage11 jpg RMAImage12 jpg erare RMAImage14 jpg My Camputer E RMAImage15 jpg A My Documents jaces M oes File name z Files of type Images SA 0 7 Open Image 130 ZILOS tk Chapter 10 Documentation 2 oOPEN Version 5 1 ZILOS tk BMP CALBRATION FACTOR IMAGE DOES NOT CONTAIN CALIBRATION FACTOR CALIBRATION FACTOR B E 0 8 Calibration Fac
193. PG IMAGE TYPE JPG BMP TIFF 77 ZILOS tk Chapter 6 The Setup Screens BROLANERES ENI 1 2 JpeG QUALITY nore RE OIF UF 1 ILIGIPAILOVARILWSLEF lt JPG QUALITY 50 80 LYE lt 80 AFRHOL TIFT Individual Image File Storage SAVE IMAGE INDIVIDUAL IMAGE FILE STORAGE IMAGE THUMBNAIL GALLERY SAvE IMAGE Individual Image File Storage Manual Ask for file name C Auto Use Report file name Auto Use Root name Root Name Count SinglelmageRoot fir 4 Reset E 6 23 BIr ILOR EWR Manual Ask for F
194. RS mer ASCII File Import File Name ZilosImport mer z File Folder FMF Data BY C 1 ASCI 151 ZILOS tk Appendix C Database Communication Field Headers ZILOS tk 5 Patient ID ID Number Name Text Embryo_ID PN ET LAL AERA ID Number YOB dg9 mm yyyy Date Date_Oocyte ddmm yyyy Date ZILOS tk FILE NAME FILE FOLDER SETUP REPORT ASCI ZILOS tk mer pz
195. Siii 0 152 Eb 155 EOS ca a a O V 151 EE 73 FPS BT ite ear 27 ee 28 8 125 100 HH 142 181 ZILOS tk Index 0 gt 4 61 106 122 143 Fe a ee en ne 117 LILLE 65 119 T 11 00 D DER A a ERR OER SI 119 MOA ae eee eo oe ae ao 126 77 68 135 P i sR 53 137 54 field headers 137 60 75 33 49 ossis 69 34 49 22 37 152 13 field headers 152 63 144 171 jpg 77 106 LAB 141 106 L
196. Z DRAWING COLOR E THICKNESS BLEKLUKADEB RIL RES HIIT OTE Sb Measure MEASURE 1 2 3 4 iF MEASURE FLAGON Relat HEORTANFT E 8 19 BHD VERT AE 118 ZILOS tk Chapter 8 The Clinical Control Panel and System Operation Thickness Drawing Color DRAWING COLOR 1 DRAWING COLOR 2 COLOR CHART PALETTE 3 BASIC COLOR DEFINE CUSTOM COLORS oc 4 DRAWING COLOR Thickness THICKNESS
197. ZILOS tk Zona Infrared Laser Optical System ZILOS tk Class Laser Product Serial Number 270658 MMOs MA UNNE Version 5 8 2009 1 20 A HAMILTON THORNE 100 Cummings Center Suite 465E Beverly MA 01915 978 921 2050 Fax 978 921 0250 sales hamiltonthorne com ZILOS tk Zona Infrared Laser Optical System
198. ation Setup Import File Fields Patient_ID Name Embryo_ID YDB Date_Docyte Save Exit Revert Cancel 75 ZILOS tk Chapter 6 The Setup Screens 1 IMPORT NAME zos tk 2 SAVE amp EXIT 3 REVERT 4 cANcEL Report Saving Options REPORT SAVING OPTIONS View SAVE CLEAR 136 Report Only REPORT ONLY REPORT FILE FOLDER jpg
199. d 49 345 52 2004 Kanyo K Kone J A follow up study of children born after diode laser assisted hatching European Journal of Obstetrics and Gynecology 110 176 180 2003 17 ZILOS tk Chapter 2 Safety Precautions and Warning McArthur SJ Leigh D Marshall JT de Boer KA Jansen RPS Pregnancies and Live Births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts Fertility Sterility 84 1628 1636 2005 Kokkali G Traeger Synodinos J Vrettou C Stavrou D Jones GM Cram DS Makrakis E Trounson AO Kanavakis E Pantos K Blastocyst biopsy versus cleavage stage biopsy and blastocyst transfer for preimplantation genetic diagnosis of beta thalassaemia a pilot study Hum Reprod 2007 Jan 29 Epub ahead of print Kokkali G Vrettou C Traeger Synodinos J Jones GM Cram DS Stavrou D Trounson AO Kanavakis E Pantos K Birth of a healthy infant following trophectoderm biopsy from blastocysts for PGD of B thalassaemia major Human Reproduction 20 1855 1859 2005 Miller KA Elkind Hirsch K Levy B Grauber MD Ross Su Scott RJ Pregnancy after cryopreservation of donor oocytes and preimplantation genetic diagnosis of embryos in a patient with ovarian failure Fertil Steril 82 211 214 2004 ZILOS tk
200. display type will require recalibrating the magnification for all objective lenses and realigning the laser target Crosshairs Report ASCII Image Capture Video Capture Video Devices Video Devices P Live Vid Audio Feedback Lumenera USB 2 0 Camera 5 fox Video 5 ettings System Video Resolutions Video Display Type lt Fill Video Area C Keep Original Size Video Frame Rate Camera Input Screen Output 15 06 fi 5 06 NV Always Attempt Input Save Exit Cancel BY D 3 Video Devices OR EHH 2 VIDEO RESOLUTION CHANGE VIDEO CAPTURE PIN Video Capture Pin Stream Format Pixel format lg bits Bayer Output size 1280960 bd Frame rate BY D 4 Video Capture Pin 159 ZILOS tk Appendix D Video Settings amp Resolutions NOTE ld e PIXEL FORMAT 8 BITS BAYER e OUTPUT SIZE 1280x960 e FRAMERATE 14 15 FPS es OK Video Devices VIDEO DISPLAY TYPE KEEP ORIGINAL SIZE LUMENERA DIGITAL CAMERA s 1600x1200
201. e ME me AG OD ITT E 175 NRN NE NE I 1 1 ZILOS tk 1 2 ZILOS tk BY 1 3 3 E 1 4 USB 3 BY 1 5 ZILOS tk 11 1 6 RED i 12 1 7 USB 13 A 3 1 a ZILOS tk ZILOS tk d 20 E 9 2 a I Ab K F c RMS HRA DA e 20 E 3 3 4 21 3 4 RED I L E 1 La USB K LY FETC 21 E 3 5 E C E SEI OASLDR HIDGIF BAK C 23 E 3 6 LK
202. icAL 2 SAVE EXIT ZILOS tk Clinical Pulse Settings CLINICAL 3 Low 0 lt t 200 us MEDIUM 200 lt t 600 us HIGH 600 lt t 3000 ns 3 PuLsE CLINICAL 100 3000 us CLINICAL clinical PULSE settings Low 1 200 us MEDIUM 200 AS 600 us HIGH 600 us 3000 us HIGH 3000 us 3000 us 6 2
203. ile Name FAD COBE IPT VEEBEAL sAvE AS Auto Use Report File Name Bz LR KDI4 NWKEF FERATS PATIENT ID ID DATE OF OOCYTE RETRIEVAL CURRENT DATE EMBRYO ID ID 6 24 nore REO SLA IA SHEE LO TH YURSNET Patient ID Current Date Counter PID1234 2005_03_17 8_21_2009 EID1234 i7 ipg Date of Oocyte Retrieval Embryo ID 6 24 Auto Use Report File Name FERLEGEOIPTIILEO PATIENT ID DATE OF OOCYTE RETRIEVAL EMBRYO ID 132 78 ZILOS tk Chapter 6 The Setup Screens Auto Use Root Name AIE Root Name ROOT NAME count ROOT NAME
204. lization Method Report Embryos per cycle Report Cycle Number 77 9 86 ZILOS tk Chapter 6 The Setup Screens VIDEO CAPTURE STANDARD TIME LAPSE 2 TIME LAPSE Standard E Setup Standard Time Lapse Laser 4 5 S ti Objectives m Video Capture File Crosshairs Manual Ask for file name Video File Name Count FepoMeo Auto Use Report file name Video h Image Capture C Auto Use Root name Reset Video Devices Audio Feedback Video File Location Capture Length System Video File Folder Time Limit C Capture A 600 m Video Compressor Video Compressor Basic Properties C Advanced LEAD MCMP MJPEG Codec Save Exit Cancel E 6 36 EFA AVI FV OBARE Video Capture File VIDEO CAPTURE FILE
205. on a AE 6Z Enable eee 68 BARNS N V AON R CaN ee aN EEN TE EES ES 69 TED RIF OT Oi asia ti vuc acs scab A es Nace oe Shs EL 69 lmages in Report L 7R KODHEZC FEA EP1 SFO RD ooieoe 71 DateFormat 73 ASCI FA ZAR RA 73 AS CLE DIG TERA ADS Psa aise O a a a a O A A Rew ots ties Aa Eaa 74 Report Saving Options L 7N KOERIF TTB EE 76 File Storage ELBA AIO FB oo cecccccccccccccssscssecscscscsvsssescsvscssvsvssscsecscssvsvasscsesscssvsvassceavscassvavscsevscnanseaveces 77 A Eb FE FD NM ES EN Ek lt ki 83 Standard RE NN 4k lt 87 Time Lapse AA RPE HE ei ia A OE NNE RE EA 9 Video BIA AE R113 92 Video Resolutions 93 Video Display Type EFJ ZEA TGEE oocccccccccccccccscscusessssescscsssesescecsacsenssesesesacsesvsvseeesacsesvsvateccscsesvavaceceecseaseae 93 Video Frame Rate 94 4 276 eee 1 95 Audio Feedback Path 95 Audio Feedback Enabled 96 Message Boxes 97 Hard Drive Free Space 97 1 Clinical ERO FAT AS Pe ss Wi SE one HOR 99 FE EO Fea EPROP ZA AOE iisihih chistes saraviciivshersivcisrieedtuacraunivieietniiavie ioapunwiesi 101 RED
206. operties 2 QJERIE MB 4 162 FE 12 Image A AMOET E A P 169 FE 13 Image A ARAO EAEE A voccccccvccccssssvessssssessssssessessssesssssssessssssvessessssussesssvussesssvcsesssvucsesssvussessssussesssvssesssnesessssusces 170 Nm 172 ZILOS tk 1 ZILOS tk ZILOS tk 2 ZELO 3 FRIAVYIBL ATF LOBES 4 GIT bh YF HBL RF LOGS 5 ZILOS tk ZILOS tk OERZ 6 JE 7 8 Clinical Clinical KF 9 RIEE F ZILOS tk Preface 10
207. ot this time Click Next to continue D 7 HLIW K 9LP ORM D1 F FE 4 INSTALL THE SOFTWARE AUTOMATICALLY NEXT Found New Hardware Wizard This wizard helps you install software for Lumenera Unconfigured Device if your hardware came with an installation CD or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Install from a list or specific location Advanced Click Next to continue E D 8 HLUW KF ULF ORHAN I1Y KO Install FRA HARDWARE INSTALLATION 163 ZILOS tk Appendix D Video Settings amp Resolutions 5 CONTINUE ANYWAY Hardware Installation The software you are installing for this hardware Lumenera Unconfigured Device has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the correct operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and BY D 9 Hardware Installation 6 FINisH
208. root NAME i 78 142 rap Cae T ne eT Ta 79 manual file NAME Hk 78 OWEN 105 Wee PER 79 Laser 53 SS 78 51 125 22 38 a eal pig LS 2 eer eee ee ene ener 125 Low 105 116 FAP JL 139 B A ree 117 Lumenera 118 161 117 158 Ea PRESE ERO OR 118 Video Device Ea 154 ER 119 Video Settings 156 ZILOS tk Index Fah estate anid 31 42 61 129 99 143 53 P E EE a 143 E a EEA ee 56 AEE AO ET 143 e E EE AE AEA R 53 122 NBIDOSISRSERONRSISINNSSONIRNSEEAISNSSESSS 57 122 eI E 54 15 setUD vcscseesscsssessssssssessssssssesssssseesssssseesssecscessuetecessnessessnesecs 54 119 video Settings ee 54 EFA REA 110 DOOM se siete Se oo 2 54 C 6 AE 128 P ee A gt Te 32 46 130 Seed k 128 coccccccccccccssssssssssssssssssssssssssssses
209. ssssssssssssesssesssseee 54 127 32 46 126 Report ASCII 70 mer 154 125 KSO ae en a ee IE 70 97 Button 1 125 Bret eA Uae 125 HEHE sot eee hen Baria ia 22 Button35EEE ene tetanus 125 MultiLaser exe 181 Button 4 125 79 auto report file name kk 79 2 135 auto root NAME q eccescceeecseecsececsesececeerecerseesrevesseterscesserersees 79 4 135 E PA AA E 80 70 manual file name 79 69 80 ASCI ar alae 70 re i E 80 AO Eh iissa 70 52 DRA E a T 136 133 UPP 136 OS IATER AETAT IATA Ers 1 134 DOi s E 1 aa 132 2 If a cae E E ee 131 OEM C 5 Renee ee ene ee ne oe 136 al acters E E ET ait 136 63 It200120 iP 136 172 91 73 74 PR 142 power 123 a 127 28 45 15
210. stallation and Start Up Desktop E 3 12 9 9 pin D 9 pin 9 pin COM 1 3 15 E 3 13 LI ROILL Y ALY KO S5 ENYA OFF RATT OTCESL f qy KO 5 EL Y ED 1 _LOREGILE DECI 29 ZILOS tk Chapter 3 Unpacking Installation and Start Up Desktop 10 11 12
211. tk 50x HOLL RN EREN TOEL 40x SL P BEE CHAU EDES ZILOS tk lass Laser Product Serial Number 270658 D MINE BY 1 1 ZILOS tk IR 0 60 NA ZILOS tk Chapter 1 Introduction to ZILOS tk A 1480 nm
212. tor EFF 6SZBXYe LY CALIBRATION FACTOR HEFITIEX 1 ELL WEFITI OIL COMED FLA Fran CALIBRATION FACTOR OBJECTIVES DK FIMEALIEMQLLY RERIN CALIBRATION FACTOR ICKEILGZVYFTF 131 11 ZILOS tk e FiLE VIEW REPORT Date 5 24 2005 Time 3 15PM Minimize Report Patient Name JaneDoe Patient ID 890287 Date of ith BFeb Es Pane 5 Diagnosis and Comments Import Save Clear Embryo Information Oocyte Embryo Fertilization Cycle Number Embryos for a Embryo ID Retrieval Date Method Cycle oo EC Clear Export DRAAI PN 1 Embryo D1 1323 D2 192 3 BY 11 1 133 ZILOS tk Chapter 11 Reports PATIENT REPORT 3
213. tus Laser Controller is Disconnected Curent Calibration Factor 0 398 Pel E 12 1 EV ODR oL B FERRI LAKES TYbKREOB OT SGK HE Clinical 3 Low MEDIUM HIGH LOW PULSE DURATION lt 10 um MEDIUM PULSE DURATION HIGH PULSE DURATION O17 um 3 100 1
214. um High a Hide Target Measurement Toolbox Tooi pe 7 ema as fre Video Record and Playback IM Time Lapse Mode te Oja m gt gt Image Capture Images E Over ne gt uto Lapture 0 Laser Fire Videos Status Laser Controller Connected on Com Port 1 EV 1 5 ZILOS tk ZILOS tk Windows 11 ZILOS tk Chapter 1 Introduction to ZILOS tk ZILOS tk RED i RED i RED i
215. yo Y LE REH 128 ZILOS tk Chapter 10 Documentation PRONUCLEI MEASUREMENT TOOLS Measurement Toolbox Toot ype SEES Clear Enter E 0 4 MH HEH DRILL MEASUREMENT TOOL F A E MEAN sD AD Measurement Toolbox Tool Type Clear Enter EQ 0 5 Drill Y LEHE PY 129 ZILOS tk Chapter 10 Documentation Ruler RULER MEASUREMENT TOOL QC Measurement Toolbox Tool Type EY 0 6 Ruler Y ILE REW COBE EIRO YARE RALEBEGITTILERIKIKIE 1 BROWSE

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