ElePhor 24S USER MANUAL
Contents
1. InterMlecicall Diagnostics ElePhor 24S AUTOMATIC ELECTROPHORESIS ANALYSER USER MANUAL REV 01 2011 ELEPHOR 24S USER MANUAL 1 0 2 0 3 0 4 0 INDEX DESCRIPTION OF THE INSTRUMENT 1 1 Standard components 1 2 Technical characteristics 1 3 Host computer requirements 1 4 Installation TURNING ON 2 1 Instrument preparation 2 2 ELEPHOR 24Smanagement software start up ELEPHOR 24S MANAGEMENT SOFTWARE 3 1 Main Panel 3 2 Starting Work 3 3 Patient Lists 3 4 Archive Management Storage Search by date Search by name Data backup Restore Data Deletion 3 5 Display graphs Delete areas Insert minimums Remove minimums Edit baseline Calibrate Fraction Highlight fraction Set fraction name Cancel Changes Print current graph 3 6 Configuration EXAM parameters VARIOUS REAGENTS 3 7 Printout Print graphs in archive Print graphs of the day MAINTENANCE 4 1 Daily maintenance 4 2 Routine maintenance Page 2 52 Nan amp Bye on 10 10 12 14 16 16 17 18 19 20 21 22 24 24 24 24 25 25 25 25 25 26 26 29 30 31 31 32 33 33 33 5 0 5 1 5 2 5 3 A l A 2 A 3 A 4 B 1 B 2 QUALITY CONTROL QC New Control Modify Control Delete Control Exec a QC statistics REMOTE HOST Setting Remote Host Info Start Selecting data from Remote Host Sending data to the Remote Host Send Database Items to Remote Host VETERINARY SC2. Values of samples are shown on the graph in the range of confidence 2s 2 s that is in the range of 2 times the standard deviation Pressing the button press produces printed copy of the QC for all these villages regardless of the currently displayed Page 39 52 ELEPHOR 24Ssoftware is capable of exchange data with a remote host in order to manage exams Work list automatically Exam Misc Reagents Remote Hast Remote Hast Inpu amp Data Path InpubFile Name NETDIR ACCETTAZIONE QutpulDatajRath tim firm F TEMP EXPRIMENET NETDIR JE Pressing the REMOTE HOST button you ll be able to indicate where remote host data are located You must first indicate the name of input file without extension this file contains the work lists after this you can use the browse button to locate your file folder The same is for the output file this file contains the results of exams Page 40 52 A 2 START SELECTING DATA FROM REMOTE HOST In order to select data from a remote host you must e Press the button START from the main screen of program e Press the button REMOTE HOST REMOTE HOST SIEROPROTEINE Items Selected fo Items Sended fo Select Items Delete Last Selection Delete All Selections Print Last Selection Print all Selections ltems Sended ltems To Send Send Items Now you ll see all available exams queued and for each exam the work list Selections are possible into t
3. ARCHIVE MANAGEMENT RESTORE Store Search by Date Z by Name Backup PE Delete e Host estore Type Entire Backup C A Year A Month Select RESTORE from the ARCHIVE MANAGEMENT menu to restore data previously stored on BACKUP media to the archive In order to restore data Select the backup unit floppy disk drive A by default Select what to restore Whole archive default One particular year only e o opne One particular month only Then select the year and or month to be restored and insert the disk labelled Restore Disk in the chosen unit for example floppy drive A Once done click the DONE button to start restoring the data If necessary insert the other numbered disks in the order in which they are requested Note button Remote Host will be explained into appendix A Page 20 52 ARCHIVE MANAGEMENT DELETION Store Bean by Date Bean by Name piety Restore Piete emote Host elete Options Delete Year Delete Month Delete Support Confira Abo Select DELETE from the ARCHIVE MANAGEMENT menu to delete all or part of the atchive First of all select the type of deletion required e Delete Year e Delete Month e Delete Support Once the support has been selected you may also choose to delete only certain graphs By default all graphs are selected for deletion To prevent certain graphs from being deleted deselect them by
4. Insert a minimum between two fractions Delete a minimum between two fractions Correct the baseline Calibrate the value of a fraction Highlight a fraction by dividing it Change the fraction label name Restore a graph Print a graph Left click one of the icons on the correction menu to select the type of correction required Right click to deselect it Page 23 52 DELETE TRACE AREAS When this button is clicked a vertical light blue line appears which may be positioned at the point where the area to be deleted is found using the mouse A yellow square appears next to the displayed graph showing the optical density O D of the trace This parameter changes with the movement of the cursor line The O D is useful when determining the position where deletion is necessary By left clicking the first half of the graph everything between the beginning of the graph and the cursor line is deleted by left clicking the latter half of the graph everything between the cursor line and the end of the graph is deleted Right clicking or selecting another button from the correction menu cancels the deletion function INSERTING MINIMUMS When this button is clicked a vertical light blue line appears which may be positioned at the point where the minimum is to be inserted using the mouse A yellow square appears next to the displayed graph showing the optical density O D of the trace This parameter changes with the movement of the cursor line The
5. O D is useful when determining the position where the minimum is to be inserted between two graph fractions Left click to insert the minimum Right clicking or selecting another icon from the correction menu cancels the minimum insertion function REMOVE MINIMUMS When this button is clicked a vertical light blue line appears which may be positioned on the minimum to be removed using the mouse Left click to remove the selected minimum Right clicking or selecting another icon from the correction menu cancels the minimum removal function A yellow square appears next to the displayed graph showing the optical density O D of the trace This parameter changes with the movement of the cursor line The O D is useful when checking the position of a minimum between two graph fractions EDIT BASELINE This function is useful for correcting supports which have undergone uneven destaining and therefore have ODs above the baseline value at the beginning or towards the end of the graph In this case the graph must be corrected so that its beginning and or end have a uniform O D value equal to zero When this button is clicked two adjustment bars appear along side the graph which may be used to move the baseline where necessaty Select one of the two bars by left clicking and adjust the corresponding cursor either using the cursor keys on the keyboard or by dragging with the mouse hold the left button pressed Adjust the other cursot if necessary in
6. The graph is restored when it is displayed Once a new support has been displayed it is no longer possible to go back and restore the original state of a previously displayed graph PRINT GRAPH Click this button to print the graph displayed Printing takes place according to the settings made by choosing VARIOUS from the CONFIGURATIONS menu Page 25 52 F Serum proteins Haemoglobin Exam Name SERUM PROTEINS Applicator Step Fractions minte _ max min gfdt Max gja amp Micro p gt ff ae a 4 __y Semimicro pret igration 10 0 mAN 4 0E20E0HIMA Td Current is Constant Multizone Select EXAM from the CONFIGURATIONS menu to configure the parameters for individual tests The following parameters may be configured on this screen for each test Test name Number of fractions and their names max 10 reference tables g dl reference table for total proteins Deposition step MICRO and SEMIMICRO Number of points to eliminate in the deposition area Migration voltage Migration current Multifractionation technique serum proteins only Method operating values Note you can change the test name in order to rename a pre defined exam Page 26 52 A new name is automatically selected for a fraction or edited after the name of the fraction itself is written If the FRACTIONS field is not completed the fraction itself is not included in the graph minimums calculation Numerical
7. left clicking Page 21 52 3 5 DISPLAY GRAPHS After preparing measuring a support its graphs may be displayed The DISPLAY menu shows a list of available supports prepared and measured By selecting a support with the mouse a preview appears where it is possible to display the contents of vetrino n 1 del 18 02 2002 SIEROPROTEINE TRACCIATO NON YALIDO TRACCIATO NON YALIDO TRACCIATO NON VALIDO the whole support and immediately see if any errors have occurred during the preparation and measurement stages In order to leave the preview of the entire support and display the individual graphs for editing or correcting simply click the mouse on one of the traces displayed Note In order to display traces retrieved from the archive select DISPLAY again from the PATIENT LISTS gt PATIENT ARCHIVE screen It is possible to display both the patient data associated with a graph and the percentage values of its individual fractions from the individual graph correction and display menu Page 22 52 Every graph has a correction menu with icons placed along side it Yetrino n 1 del 18 02 2002 SIEROPROTEINE AMARI LETIZIA 101234 roam 50 Sesso F 2 Prelievo del Reparto a 22 5 10 0 Note di Commento Questo un Commento Ogni riferimento a persone realmente esistenti da ritenersi casuale l Ferogramma mr From the menu it is possible to Delete a trace atea
8. left part shows the sample grid seen from above and the right consists of buttons for the methods and functions which may be carried out support preparation support measurement or both The sample grid is arranged in rows from top to bottom Therefore row 1 corresponds to support 1 row 2 to support 2 etc the position of the first sample in each row is indicated by a yellow dot Therefore by selecting a row from the sample base you also select the position of the support to be used f f Unselect All 3 i j Serum pyoteins Haemoglobin Urinary proteins aa gaeane i inn f amp amp amp ie Lipo proteins i dl ee v Prepare Supports v Read Supports J Read Suppo All you have to do after that is select the type of test required by clicking the corresponding button Note that by default the MS is set for support preparation and its subsequent measurement the two checkboxes are selected Therefore if you only wish to do one of the two functions deselect the option concerned by pointing the mouse at the checkbox and left clicking the box is no longer selected Page 12 52 e Once the supports samples to be used and the test to be performed have been selected a button marked Start appears By clicking this button the chosen process starts and a status screen appears which shows the progress of the various stages of the method for each support as well as data regarding certain parts of ELEPHOR 24S migratio
9. percentage values are expressed as for example 12 7 i e with only one decimal place and a decimal point separator The program beeps if characters or numbers with a different format are typed in After inputting the min max reference table a congruity test may be carried out by clicking the T key found beside the table Any errors found are automatically highlighted in sequence by a box which appears on the cell s containing the incorrect value s The min max total proteins reference values in g dl together with the min max reference table determines the min max reference table in g dl for the single fractions For example Min ALBUMIN value in g dl Min g dl value for TOTAL PROTEINS Min value for ALBUMIN If a SEMI MICRO deposition step is chosen only four traces may be selected choose CURRENT DATA from the PATIENT LISTS menu The odd traces are available for measurement whereas the even ones are automatically defined as NOT VALID On this screen you can choose whether to carry out constant voltage or constant current migration for the type of test concerned and set the appropriate value Clicking the METHOD button displays a new screen where the time of operations performed by ELEPHOR 24S may be edited All times are in seconds changing these times changes the method and affects the quality of the traces produced The default method parameters are a good compromise between process speed and res
10. 150 ml of buffer solution until the compartment level reaches the reference line level off the quantity between the two compartments 4 Put the electrophoretic chamber back into its slot and push home 5 Pull out the sampler and pipette 25 30 ul of sample into each well 1 fig 2 1 1 1 Fig 2 1 1 6 Fill the depositor washing compartment 2 with 1 ml of bidistilled water 7 Insert the special strip of absorbent paper in the compartment 3 in position E on the treatment rack 8 Put the sampler back in place and push home 9 Place the applicator on the support plate in position D Fig 2 1 2 10 Place the supports to be prepared on the support plate 1 3 in Fig 2 1 2 11 Run the ELEPHOR 24Ssoftware to start the operating cycle Page 8 52 2 2 ELEPHOR 24S MANAGEMENT SOFTWARE START UP Turn the computer on and let the operating system Windows 9x start up When the Windows Desktop appears fig 2 2 1 click the start button and choose ELEPHOR 24Sfrom the menu or point the mouse at the ELEPHOR 24Sicon on the Windows Desktop and double click Note to avoid visuals artifacts set Windows Desktop theme in Windows classic mode ELEPHOR msaasa esea as Follow the instructions in section 3 2 to start working Page 9 52 The Management Software provided with the ELEPHOR 24S analyser allows electrophoresis traces to be selected customized corrected stored and printed through an intuitive hierarchica
11. 2 1 4 INSTALLATION The instrument must be placed on a perfectly flat surface in a room with a temperature of between 15 C and 30 C and humidity of 40 80 max Make sure the ventilation slits are not obstructed and leave at least 10 cm of space behind the back panel Remove the lock screw from the mechanical arm before use Connect the USB cable provided to USB connector on the left panel of the instrument fig 1 4 1 and connect the other end to the computer USB hub RESET Button Link to P C USB fig 1 4 1 Power Plug Main Power Switch Connect the power cord to the Power Plug Connect the five connectors on the back of the instrument fig 1 4 2 using the tube provided D 0 9 8 Whete 1 stainer tank 2 destainer tank 3 buffer tank 4 washing solution tank and 5 waste tank Page 6 52 2 0 TURNING ON To turn the instrument on press the power switch on the left panel of the device the system starts to move back to the home position Note during the first activation be sure to remove the lock screw from the mechanical arm Page 7 52 2 1 INSTRUMENT PREPARATION Before starting an electrophoresis process prepare the electrophoretic chamber in the following way 1 Pull the electrophoretic chamber out from its slot by the sides Place the two floats in the electrophoretic chamber and fasten them using the support with holes provided 3 Fill the two compartments with
12. IENCE MANAGEMENT Configuring species Managing species TROUBLESHOOTING REMOTE HOST INTERFACE SPECS Page 3 52 34 35 36 37 40 40 41 42 43 44 44 46 47 50 10 DESCRIPTION OF THE INSTRUMENT ELEPHOR 24Sis able to perform the electrophoretic procedures of the following clinical tests automatically Serum proteins Haemoglobins Lipo proteins Urinary proteins furthermore additional tests may be created and customized such as Serum protein multifractionation ELEPHOR 24S uses supported cellulose acetate strips which are managed completely automatically Simply insert them in the appropriate slots and tell the machine which strips to use The patient archive is stored on an external computer HOST connected to the machine through a standard USB cable The number of patients which can be stored in the archive never less than 100 000 in any case therefore depends on the capacity of computer s magnetic storage medium HARD DISK 11 STANDARD COMPONENTS ELEPHOR 24S comes complete with the following standard components Eight thin plate serum applicators for MICRO methods 24 place sampler 3 supports x 8 samples 3 supports for cellulose acetate strips 1 reagent tank with two compartments for automatic reagent handling Usb cable Power cord Destainer washing and waste tank connection tube external diameter 5 mm internal diameter 3 mm One reagent tank buffer
13. M M Support 1 19 11 2004 Select PATIENT ARCHIVE from the PRINT menu to print the support selected from the patient archive When this option is selected all the valid graphs for the support are selected for printing Graphs may be deselected by left clicking beside the traces which you do not wish to print Once selection is complete click DONE to start printing Printing may be interrupted at any time by clicking the CANCEL PRINT button on the top right of the screen Page 31 52 ji From Database stork List Support 1 Support 2 Support 3 Confit Select CURRENT DATA from the PRINT menu to print the supports produced during the operating cycle When this option is selected all valid supports and graphs are selected for printing Supports and or graphs may be deselected by left clicking beside the supports or graphs you do not wish to print Once deselection is complete click DONE to start printing the graphs Printing may be interrupted at any time by clicking the CANCEL PRINT button on the top right of the screen Page 32 52 4 0 MAINTENANCE 4 1 DAILY MAINTENANCE At the end of each work session it is advisable to carry out the following cleaning operations Wash the serum plate under running water and dry with absorbent paper Empty the electrophoretic chamber and recycle the buffer remove the floats from their housings rinse them with deionized water and leave them to dr
14. Manuals ELEPHOR 24Ssoftware CD ROM Page 4 52 1 2 1 3 TECHNICAL CHARACTERISTICS Power supply voltage 220 V 50Hz Power consumption 250 W max Automatic reagent loading and handling Sample refrigeration by a Peltier cell Reagent buffer and stain recycling upon job completion Pluid circuit washing routine upon job completion Electrophoresis power supply unit with voltage 100V 300V and current regulation 7mA 30mA Pneumatic support handling Operating capacity of 16 tests per hour Measurement with non cleared supports Dimensions WxHxP 56x46x54 cm Weight 30 Kg HOST COMPUTER REQUIREMENTS The minimum configuration for correctly interfacing with the ELEPHOR 24Ssystem is 700 MHz PENTIUM III PC or equivalent 128MB RAM 1 2 Gbyte hard disk 800x600 resolution 65000 colour video adapter 15 monitor 2X CD ROM drive Windows XP SP1 operating system Recommended configuration 1200 MHz PENTIUM III PC 256MB RAM 1 2 Gbyte hard disk or better 800x600 resolution 16 million colours video adapter 17 monitor 2X CD ROM drive or better Windows XP SP3 operating system or better About 30MByte of free space is needed on the hard disk for installation The remaining space may be used for the patient archive until full Note to avoid visual artifacts set Windows Desktop theme in Windows classic mode and set numeric format in 0 00 notation using dot as decimal separator Page 5 5
15. N network serial connection etc physically connected to the analyser and the remote host Such operation does not need the information provided herein and is pertaining exclusively to the final user and or the host program providers Interfacing the two computers requires the compliance with the standard rules of LAN connections or other management specifications allowing text file exchange and folder sharing by two or more computers please note that if you have computers running under Windows 2000 XP or Linux you may need to configurate the user accounts with administrator rights In general you just need to share the NetDir folder of the computer the analyser is connected to and a folder of the computer the host is part of such folder will be indicated in the ELEPHOR 24Snet program configurations Coding of acceptance data Acceptance takes place through a text file in the NetDir folder sent by the remote computer such file will have a fixed name followed by the extension txt typical of text files Eg Acceptance txt The acceptance host software shall take exclusive care of the removal of such file from the NetDir folder the updating of the data contained therein shall be made concurrently by the two software ELEPHOR 24S and the host program consequently we recommend the use of methods allowing to synchronise the file access by the acceptance host software Acceptance file data format The acceptance file is an unformatt
16. PROTEINE Support 8 SIEROPROTEINE Support 9 SIEROPROTEINE Confirm JKKK When this option is selected all valid supports are selected for storage Deselect any supports you do not wish to save by left clicking beside them Once selection deselection is complete click DONE to start saving the graphs in the archive Page 16 52 ARCHIVE MANAGEMENT SEARCH BY DATE Select SEARCH BY DATE from the ARCHIVE MANAGEMENT menu to search for a support in the archive by its storage date Select the year then the month then the day concerned a list of supports stored on the date input appears Scroll through the list to find the support required Store Eain by Date Beareh by Name Backup Restore Delete Remote Host ADDON 1 SIEROPRO TEINE i iaa aa Support 2 SIEROPROTEINE Support 9 SIEROPROTEINE Once selected click DONE to load the support The patient data for the chosen support may be displayed by choosing PATIENT ARCHIVE from the PATIENT LISTS menu Page 17 52 ARCHIVE MANAGEMENT SEARCH BY NAME Store Beare by Date rath by Name Backup Restore Delete emote Host 123456789 SIEROPROTEINE 1271704 444123456 SIEROPROTEINE 1271704 LOL123455 SIEROPROTEINE 1271704 SIEROPROTEINE 1271704 SIEROPROTEINE 1271704 ACABEBACC SIEROPROTEINE 1271704 SIEROPROTEINE 1271704 SIEROPROTEINE 12 1 04 Select SEARCH BY NAME from the ARCHIVE MANAGEMENT menu to search for a su
17. Problem Probable cause Soluzione Too high migration current over 10 mA Migration at constant voltage Wrong dilution of the buffer too much concentrated Acetate membrane too much wet before migration Replace the buffer solution correctly diluted 1 10 Increase the drying time 1 in the method parameter configuration up to 120 sec Too high migration voltage over 250 volts Migration at constant current Too dry acetate membrane One or both wicks are glided down respect their correct position Decrease the drying 1 time on the method parameters configuration up to 30 sec Replace the wicks assuring the correct position Distortion on migrations One or both wicks are glided down respect their correct position Too high buffer solution level in the electrophoretic chamber Acetate membrane is too wet before the migration Replace the wicks assuring the correct position Recheck the buffer solution level in both sides of the chamber up to reach the contact with the wicks total buffer volume 150 ml equally distributed in the sides of the electrophoretic chamber Increase the drying 1 time on the method parameters configuration up to 120 sec Insufficient staining of the fractions too low alpha1 fraction Exhsausted staining solution Destaining solution too diluted Too low quantity of samples in the wells Replace the staining solution Replace the destaining solution with w
18. R 24S software when such patient is accepted lt Sentflag gt boolean flag indicating the occurred dispatch by ELEPHOR 24S software of the report data relating to that patient It is set at lt F gt False by the host the first time the acceptance file is sent and is modified into lt T gt True by ELEPHOR 24S software when the report data are sent Typical line examples of an acceptance file please note that the use of characters lt gt is only the result of an agreement in order to highlight the used fields and characters they are not shown in the text file Acceptance of Seroproteins MARIO ROSSI33 M 15 09 02 0001 6 0 HAEMATOLOGY 1 F F lt CR gt lt NL gt when the test is accepted the line becomes MARIO ROSSJI 33 M 15 09 02 0001 6 0 HAEMATOLOGY 1 T F lt CR gt lt NL gt when the test is sent to the remote host the line becomes MARIO ROSSI 33 M 15 09 02 0001 6 0 HAEMATOLOGY 1 T T lt CR gt lt NL gt Now the report data of the patient MARIO ROSSI are available and the remote host may retrieve them from the report file For partial acceptance for example with no information about age gender Total Proteins and department you would obtain the following MARIO ROSSL lt SP gt lt SP gt 15 09 02 0001 lt SP gt lt SP gt 1 F F lt CR gt lt NL gt When the field lt Sentflag gt becomes lt T gt the data have been keyed in the report file and may be decoded and processed by the host Page 51 52 Report file spe
19. TEINE 12 1 04 LOL1Z3455 SIEROPROTEINE 12 1 04 SIEROPROTEINE 12 1 04 SIEROPROTEINE 12 1 04 ACABBBACC SIEROPROTEINE 1Z 1 04 SIEROPROTEINE 12 1 04 SIEROPROTEINE 12 1 04 To send Database items to a remote host you must e Select the button Database from the main screen Press the button Remote Host Search the items to send using the name exam and data filter Select items you want to send Press the button Send Page 43 52 APPENDIX B VETERINARY SCIENCE MANAGEMENT This appendix describe program items to manage animal species by using a dedicated software program version V All things described for the standard version are valid too B 1 CONFIGURING SPECIES Using the Settings window you can create for each exam one or more species with a particular range of normal values and a particular number of fractions max 10 fractions Note Veterinary program version doesn t allow the creation of a user defined exam Ben Varig ME Reagents Host Remoto f Sieroproteine Emoglobine Esame Selezionato SIEROPROTEINE Pet dieataa asso di Lettura 5 9 Micro SPECIE CANE Semimicro pe EFAULT 2 igrazi DEFAULT 1 igrazione _ ii 1 DEFAULT 3 D EFAULT 4 EFAULT 5 z 10 0 MAR C A 0ER20FE0IMA e Id Migra a Corrente Costante a PO Proteinemotall Multifrazionata Pressing the button SPECIES will appear a drop down menu where you can 1 Create a
20. cifications The report file is organised very similarly to the acceptance file an unformatted text file containing the patient s data and the report data including the scan data Individual report line data format lt Name gt lt age gt lt gender gt lt data gt lt Idcode gt lt TP gt lt department gt lt Testcode gt lt Acceptedflag gt lt Sen tflag gt lt Testname gt lt RefMinPT gt lt RefMaxPT gt lt NumRef gt lt RefMinPct1 gt lt RefMinPct NumRef gt lt RefMaxPct1 gt lt RefMaxPct NumRef gt lt NumVal gt lt ValPct I gt lt ValPct NumVal gt lt Labell gt lt Label NumVal gt lt NumMarks gt lt Mark 1 gt lt Mark NumMarks gt lt NumPoints gt lt Point1 gt lt Point numPoints gt lt CR gt lt NL gt The same rules of the acceptance file are applicable and the initial fields are identical to those of the acceptance file Now let us examine the report specific fields lt Testname gt string indicating the test name as it is shown on the analyser lt RefMinPT gt float string type min reference value for Total Proteins in g dL format 0 00 lt RefMaxPT gt float string type max reference value for Total Proteins in g dL format 0 00 lt NumRef gt this value indicates the number of elements forming the test reference table followed by NumRef values being the percentage minimum reference values followed by NumRef values being the percentage maximum reference values All va
21. dow is active USB cable is not connected to the P C correctly Turn on the analyzer Reset the analyzer by pressing the Reset button and re run the software Control the connection of the cable between the analyzer and the P C host Serum sample is not in appropriate quantity or it is not uniformly dispensed in the serum Remove the serum samples from the plate and re add well distributed a sufficient electrophoretic chamber Acetate membrane is bad mounted on the plastic frame 2 Samples are not applied correctly on plate quantity 25 microliters each the acetate plate Tips of applicator are not independed the Clean the applicator assuring the one from the others indipendent moving of each tip Damaged applicator tips Replace damaged tips with new one Acetate membrane is too wet before the Increase the drying 1 time on the method application of samples parameters configuration up to 120 sec increase the application time up to 25 sec Insufficient migration current One or both wicks are glided down respect Replace the wicks assuring the correct their correct position position 3 Insufficient buffer solution level in the Refill the buffer solution in both sides of the chamber up to reach the contact with the wicks total buffer volume 150 ml equally distributed in the sides of the electrophoretic chamber Assure the correct mounting of the membrane on the plastic frame Page 47 52
22. ed text file the lines of which are the acceptance data of an individual test in the following form lt Name gt lt age gt lt gender gt lt date gt lt Idcode gt lt TP gt lt department gt lt Testcode gt lt Acceptedflag gt lt Sen tflag gt lt CR gt lt NL gt The following rules are applicable Each line consists of a set of 10 ten string type fields separated by a comma Each line ends with a character lt CR gt Carriage Return and a character lt NL gt New Line Each field must be indicated for the fields that are not desired just key in a character lt SP gt SPace and maintain the correct sequence as above specified Field meaning lt Name gt Name of the Patient the test is relating to max 30 characters lt age gt age max 3 characters lt gender gt gender 1 character M F lt date gt date related to the test in dd mm yy format this field must be compulsory lt Idcode gt patient identity code max 9 characters lt TotalProteins gt float type value format 0 00 accounting for total proteins lt department gt name of relevant department max 25 characters Page 50 52 lt Testcode gt test code 1 Seroproteins 2 Haemoglobins 3 Lipoproteins This field is COMPULSORY lt Acceptedflag gt boolean flag indicating the occurred acceptance of the test relating to that patient It is set at lt F gt False by the host the last time the file is sent and is modified into lt T gt True by ELEPHO
23. ell diluted one dilution is 1 20 Remove the serum samples from the plate and re add a sufficient quantity 25 microliters each well distributed Inhomogeneous destaining of the acetate membrane background Destaining tank empty during the analytical cycle Check before the startup of the machine the presence of enough destaining solution in the external tank for a complete cycle of 3 membrane the minimum quantity of 500 ml is required Page 48 52 Truncation of the gamma fraction in Back migration of the gamma zone fraction Decrease in the params configuration the 9 the graph during the migration deposition line value up to 130 and re scan the membrane before to re scan please assure the complete drying of the membrane Gamma fraction is moving forward Too high migration Tension Currente at See points 4 and 5 10 respect the application point the startup Page 49 52 REMOTE HOST INTERFACE SPECS Remote Host Specifications The data relating to a test and to the patient related thereto are sent by a remote computer host by sending a text file to the NetDir folder of ELEPHOR 24S management program located on the local PC connected to the instrument The coding of the data contained in such file will be explained later in this section Requirements in order to properly interface ELEPHOR 24S analyser with a remote host first you have to network the PC by a LA
24. firm First create a control sample pressing the button New Control after selecting the exam by the drop down menu and fill in the text box Control with the name that identify that caontrol Fill in the bottom grid from left to right with the theoretical values of the control and click on Confirm Page 35 52 5 2 MODIFING CONTROL DATA Once you create the control is always possible to change the values expected in the following way e Select the exam e From the control box select the name of the control to change e Once you pop the grid values select the Edit button and proceed to the modification of values in the grid Quality Control TEST CONTROL1 ba SIEROPROTEINE hd Modify Control E SIEROPROTEINE x New Control Delete Control Referenc lues Se Ea e FE AS a 2 0 7 8 12 4 17 6 Search 50 2 Confirm Click on Confirm to store the modified data Page 36 52 5 3 DELETING A CONTROL To delete a control from the list of available controls select the Exam and the control name as done in the previous paragraph once the pop up screen New Control Delete Control ren es a ae eee ee ce 2 0 7 8 12 4 17 6 Search Confirm Click on Delete Control and press Confirm to submit the operation Page 37 52 5 4 EXECUTION OF A QC STATISTICS To perform a useful statistical QC
25. for a monthly or annual review and select the control you want to make statistics and press Search LOTTO XYZ PATOLOGICO bs Nuovo Controllo SERUM PROTEINS sf Elimina Controllo Ricerca E egui oc Cancella Annulla Annulla It will appear a box with a list of all the years of work in which the reference control was used clicking the right mouse button on the year with a series of options appear e Run QC QC statistics starts and displays the result e Delete delete the selected item Year Month from the QC e Abort Closes the menu If the entry for the year you left click mouse a screen appears containing the list of months the year selected which was used as the reference standard Controllo LOTTO XYZ PATOLOGICO bd Nuovo Controllo Esame SERUM PROTEINS sd Elimina Controllo Ricerca FEBBRAIO20 MARZO2007 Cancella 4nnulla Annulla Page 38 52 Now right click mouse menu appears as described above from which to start statistics on the option with the click left button Coeff Variazione 15 01 07 Stampa Annulla Controllo Eseguito su3 punti The screen displays the average values for each reference sample relative to the day of the month in which it was performed for each portion of the examination in which the QC will appear Reference value Median Variance Diversion CV The total number of points on which it is performed to Statistical
26. he right box clicking on the little box on the left of the patient name by the left button of the mouse Press the right button to select all patient listed into the box When you have selected some patients press the Select Items button to add this selection to the sample grid Note you can select only a single exam list per operative cycle Pressing the button Delete All Selections all elements selected for that exam will be deselected pressing the button Delete Last Selection the last elements queued are deselected To obtain a printed work list press the button Print All Selections Page 41 52 A 3 SENDING DATA TO THE REMOTE HOST REMOTE HOST SIEROPROTEINE Items Received fi Items Selected fo Items Sended fo Select Items Delete Last Selection je eee ei ad Delete All Selections pea ea Print Last Selection Print All Selections Items Sended Items To Send Send Items To send the results of an exam to the remote host you must Press the button Start from the main screen an then select REMOTE HOST Press thebutton Items To Send Select items into the box Press the button Send Items It s also possible to send data from the local database to the remote host see next session Page 42 52 A 4 SEND DATABASE ITEMS TO REMOTE HOST Store Eee by Date Ee Name Backup Restore Delete Bemote Host ID EXAM DATE 123456783 SIEROPROTEINE 1Z 1 04 4B4123456 SIEROPRO
27. l organization Every function or group of functions is brought together on a single screen which can be selected from the main panel by simply clicking the corresponding button Geir seit Bfoewocierosis J yai versie hii Database Work Lists m Start s Corrections Settings 2O 2 ET EE 1 Archive Management Button 2 Patient List Button 3 Start Button 4 Graph Display Button 5 Configuration Button 6 Print Management Button 7 Quality Control Button Page 10 52 Before starting a work routine you must Log in into the software default user name ADMIN with password admin and choose the type of test and the number of supports the sample to be analysed is to be deposited on The position of the support corresponds to the position of the samples contained in the sample holding base To select a test and the samples to prepare and or measure carry out the following procedure on the Management Software hereinafter referred to as MS main screen e point the mouse at the Start button and left click ei EG stead Elasin Jyly garaje Lij A ER EEES i Work Lists Y Corrections Settings Print Page 11 52 the following screen appears Unselect All Serum proteins Haemoglobin Urinary proteins Saag agueaeme inne ft tm Lipo proteins ete oe Td Prepare Supports Yd Read Supports BB Read Supports Immediate the screen is split into two areas The
28. lues of reference tables are of float string type in format 0 0 so following the lt NumRef gt field there will be 2 NumRef values forming the percentage reference tables To retrieve the reference tables expressed in g dL use the parameters RefMinPT and RefMaxPT with the percentage Min Max reference tables lt NumVal gt Number of elements forming the table of the read concentration values such value takes also into account a fraction partitioning values Then there area NumVal values being the percentage concentration values All concentration values are of float string type format 0 0 These are followed by NumVal strings lt Labell gt lt Label NumVal gt representing the names of fractions and partitioning please consider that the partitioning names are always preceded by a character lt gt at In order to obtain the concentration values expressed in g dL use the TP value if available and the percentage concentration values lt NumMarks gt Number of elements being fraction marks followed by NumMarks values of full type representing the abscissa where the fraction marks are shown such values are associated with the scan data values lt NumPoints gt Number of points being the scan followed by NumPoints values of full type being the scan curve The report file name reflects the rules of the acceptance file it will be of Report txt type The host management program takes care of the removal of such file from the folde
29. n voltage and current and the levels of the reagents in the tanks Note it is advisable to wait until ELEPHOR 24S has carried out all the initialization operations before leaving the MS workstation unmanned if necessary since error messages may appear which must be corrected by the operator so that the processes may be restarted correctly One typical error which may occur is insufficient reagent in the storage tank In the case of a method with automatic reagent level handling this results in failure to reach the operating level and leads to the corresponding error message Once the ELEPHOR 24S has started up correctly the MS workstation may be left unmanned In order to correct an error the operator must fill the corresponding reagent storage tank click the Continue button on the error screen and click the Start button on the main screen again Note button named Remote Host will be explained into appendix A Page 13 52 This button opens a screen with information relating to the patients to be tested Up to 24 patients may be managed at a time 8 patients x 3 supports plus eight other patients which may be retrieved from the archive using search functions ins lrlinlincre Actttalllis Support 1 Support 2 Support 3 Name v cge m n a j L N The PATIENT ARCHIVE menu contains the data relating to the support selected from the archive The patient s data name surname age etc and total pro
30. new species 2 Select a species 3 Delete a species You can create a species by pressing the button SPECIES and choosing Create Species it will be created a new table of normal ranges and a new label where you can input the name of the created species Page 44 52 To delete a species press the button SPECIES and choose Delete Species it will appear the list of species available where you can select the item to delete Note the default species cannot be deleted but can only be modified in all its parts Page 45 52 B 2 MANAGING SPECIES Species management can be done for each sample by selecting the appropriate species item into the Work Lists window Where not stated default species parameters are used Samples recalled from the database not allow species to be changed Archivio Pazienti We Attiali Stampa lista r Supporto 1 Supporto 2 Supporto 3 Molina ive T oF T F pecs a 25 Jo F TEST3_6 frests oo eyes ELETTROFORESI BELLE SIER PROTEINE To change a species on a sample click on the drop down list button into the field SPECIES and select the species you want To change the species all eight samples in one pass click on the button SPECIES and make your selection Page 46 52 APPENDIX C TROUBLESHOOTING Problem Probable cause Solution 1 Analyzer not linked to the P C Host The analyzer is turned off The analyzer is on and the Busy indicator on the status win
31. pport in the archive by the patient s name the patient s exam or id code any combination is valid Type the patient s name into the appropriate field to start searching the result is highlighted in blue Left click the result to select the patient found along with the types of tests concerned and their storage date and press Confirm to display it The patient data relating to the graph and support selected may be displayed by choosing PATIENT ARCHIVE from the PATIENT LISTS menu Page 18 52 ARCHIVE MANAGEMENT BACKUP Store Bean By Date Bean by Name Bk Restore Delete emote Host C A Year amp Month It is advisable to save the archive data periodically on a floppy disk or backup unit for example IOMEGA ZIP Select BACKUP from the ARCHIVE MANAGEMENT menu to save all or part of the data archive In order to make a backup 1 Select the backup unit floppy disk drive A by default 2 Select the type of backup e Whole Database default e One particular year only e One particular month only Then input the year and or month to be saved Once done click the DONE button to save the data on the backup media selected for example floppy disk A In the case of floppy disks the backup procedure may require more than one disk it is advisable to number each disk used during the procedure The last disk must be labelled Restore Disk and may be used to restore the data saved if necessary Page 19 52
32. r Page 52 52
33. reserved for the header of the printed report it s possible to modify font size and text formatting Notes at Bottom of Page this space is used to print fixed text at the bottom of values Additional Line at the Bottom Of Page a single line can be typed in which is printed under the footnotes with the same layout as used when writing Print Report in Colour it is possible to choose either a colour or black and white trace printout This selection only refers to the trace printout Depositions are normally printed in red Mark Values out of Range this option prints any fraction values which are out of the normal range in bold Kit Management it s reserved for analyzers with smart card reader Page 29 52 e Remarc tsact nN Gc 6 i es Reagents Recycle Clean MENEGES Singe RE e ene It s not a real configuration but it offers a way to manage some operations on Reagents individually Fill Flush Recycle In this screen it is also possible to Recycle all Reagents Recycle button or to Recycle Reagents and Clean Sides Recycle Clean button VERY IMPORTANT NOTE YOU MUST BE SURE THAT ALL CONNECTORS OF SIDES ARE PLUGGED IN ANYWAY IT MAY RESULT IN DAMAGES TO THE CIRCUIT OF REAGENTS Page 30 52 The whole printing process may be set up in one place on this screen where the graphs to be included in the report may be selected UNKNOWN JHON NONAME MICHAEL NAMELESS JOLANDA M M
34. teins value may be edited The SHOW button displays the graphs which correspond to the selected support Any corrections or changes to the traces displayed may be made on this screen Page 14 52 Acttalllus Support 1 Eresi eae 3 Select CURRENT DATA from the PATIENT MANAGEMENT menu to input the patient data corresponding to the pherogram to be measured Once this function is chosen the set of selected supports appears and it is possible to input patient data for all the supports displayed Simply type the surname name sex sampling date the default is always the current data patient ID total proteins and department into the Patient Data table The Q C field is used to select a previously defined Control Page 15 52 3 4ARCHIVE MANAGEMENT This screen provides complete control over the report archive Patients may be searched for by name or by storage date reports which have not yet been stored or edited may be saved and data may be backed up on removable media or deleted ARCHIVE MANAGEMENT STORE Select STORE from the ARCHIVE MANAGEMENT menu to save the supports measured along with the corresponding patient data in the archive a Store Bean By Date Bean By Name Backup Restore Delete emote Host SELECT SUPPORTS TO STORE Support 1 SIEROPROTEINE Support 2 SIEROPROTEINE Support 3 SIEROPROTEINE Support 4 SIEROPROTEINE Support 5 SIEROPROTEINE Support 6 SIEROPROTEINE Support 7 SIERO
35. the same way Once cursor adjustment is complete click the button again and correct the graph baseline obtained by moving the end points of the trace onto the zero O D line CALIBRATION FRACTION The value of a single measured fraction may be calibrated applying a calibration factor When this button is pressed a grid of values will be displayed Set the single calibration factor by changing the value and pressing the Enter key to validate it After you changed the values click on Confirm to apply it Page 24 52 SET FRACTION NAME Click the grid containing the fraction names to display an input box where a new name for the fraction or sub fraction may be input INSERT NEW FRACTION Right clicking on the grid containing fraction names it will be displayed an input box where setting a new fraction name and a new optional range of values in pct The new added fraction will be placed where the right mouse button was pressed HIGHLIGHT FRACTION This function highlights certain fraction characteristics by dividing it into several areas maximum of 3 In this way precise information may be obtained regarding the concentrations of certain pathological components e g monoclonal peaks CANCEL CHANGES If you make irreversible mistakes while correcting a graph for example when correcting its baseline or deleting an area it is possible to restore the graph as measured Click this button to restore the graph to its original state
36. ult quality It is therefore unadvisable to change these settings unless you are fully aware of what you are doing Page 27 52 EARE aR a Ls 720 44 aq Ez a ad Application Line Filet a Breer AM nioln Peele Parameters modification related to tracks editing are shown on this screen e Application Line by decreasing this value more points at the end of the track will be displayed needs membrane re scanning e Filter Calibrator allows to change the filter parameter used by the instrument to compensate variation density of the acetate membrane default value is 23 Change of this parameter is password protected PW device and should be performed only by authorized technical service personnel It is higly reccomended not to modify this parameter unless to be able to prove its correctness by means of a proper serum calibrator e Calibration Factors allows to set a calibration factor for each fraction defined into the exam Page 28 52 his is a sample Te RIES ek Eon Ot PETS Aabi Ume Ete toe ro Or Eee it managemet _ Pri f md Print Logo O None Automatic yd Print Report in Colour Wil Mark Values out of Range Inactivity time joo gt Select the VARIOUS button from the CONFIGURATIONS menu to edit the printing and instrument connection parameters REPORT PRINTOUT CONFIGURATION PARAMETERS PAGE HEADER this is the space
37. y Remove the acetate supports from the strip racks Clear the depositor by a soft bristle brush 4 2 ROUTINE MAINTENANCE It is advisable to wash the following parts more carefully at regular intervals Migration chamber fill the chamber fill it with distilled water and leave it for an hour Clear the buffer side Remove the buffer tank and put a tank with a 10 hypochlorite solution From the Reagents Menu click on the buffer Fill button and after a couple of seconds press the Recycle button Immerse the serum plate in 10 hypochlorite solution for an hour and rinse with distilled water Page 33 52 Here must be defined all Samples used for control purpose It s possible define one or more samples for each exam indicating the average value attended for every fraction When a Control is defined it s possible to select it by the Q C dropdown menu into the List of Patient screen New Control SIEROPROTEINE Page 34 52 The first thing to do to properly use the QC management is to select the type of exam from the drop down Exam In this menu we offer exams currently configured in the software click the mouse on the exam for which you want to make a QC or where you want to add a new control By default the software comes with no parameters related standard samples as this is made by known sample for the laboratory or using special kits New Control f Con
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