Leica Epi colour instructions March 2014
Contents
1. 24 03 14 OPERATING INSTRUCTIONS LEICA pms5000 epi fl microscope You must not operate this equipment without prior training from a BALM facility staff member To arrange training and for help please contact Facility Manager Dr Ann Wheeler ext 2406 a p wheeler qmul ac uk Microscopy Technologist Isma Ali ext 2407 i ali qmul ac uk Standard Operating Procedure Colour How to turn the equipment on 1 Switch on the Leica controller box 2 Switch on the computer and log in 3 Make sure the lever on the RHS of the microscope is in How to turn the equipment off 1 Switch off the computer 2 Switch off the Leica controller box Rules of use This microscope should be treated with respect and care at all times This Microscope can be used by Masters Research or PhD students Postdocs and members of staff independently after training MBBS and BSc students must be supervised by a member of research staff at all times The microscope lenses must be cleaned after every usage If you have any problems at all with the microscope no matter how trivial they may seem please see a BALM facility staff memberimmediately REMEMBER You have 5GB of disk space on this microscope Check before you start if you have room for your experiment If not delete your old data 24 03 14 1 Open LAS software 2 Place slide on microscope stage and choose objective lens 3 Make sure the rod on the RHS of the microscope is push2. ed in This sends the light to the colour camera 4 Check rod on LHS is pushed IN to direct the light to the eyepiece ee Acquire 5 In Acquire select the Mic1 tab and BF Contrast Methods TL BF v TL Axis r IL Axis Combi Illumination B H 23 6 Open the shutter and find your sample 7 Pull OUT rod on LHS to direct light to the camera NB The software takes a while to load Objectives available 4x 10x 20x 40x air 40x and 63x oil Move by hand not automated NB please LOWER THE STAGE before changing between objectives to avoid crashing lens onto slide This section tells you which objective you are using These dials control aperture field diaphragm and light intensity check they are not zero 24 03 14 8 In the Camera tab choose the DFC490 camera 9 Inthe A tab select the Bright field configuration 10 Adjust the Exposure and Gain LAS Core File Options Help Gain Noa Saturation SSS SS Gamma _ DFC 350 FX R2 003922005 DFC 490 235792407 This button can be used to open and close the shutter Rhod BP 136 4 10 T Rhod LP 3 59ms 32 BF BF 418ms 1 0 CY5S ARi 1864 1 0 DAPI BP 356 9 22 DAPI LP 186 4 10 FITC GFP 3508 Gamma should be set at 0 6 for colour images Configuration Bright field a Manual acquire E Auto create overlay B Always create new overlay folder Default Sequence Mame Delete I
3. mage Empty Sequence 24 03 14 11 In the Camera tab use Auto white balance on a blank area of your slide 11 In the Browse menu select a folder to save your data by clicking the Set capture location button 16 In the Acquire tab click Acquire Image If needed there is an auto exposure tool here Data should be saved on the D drive Folder selected is indicated by the red DATAPARTI D dot gt mi Drivers gt mi ImageJ gt mil Leica stuff please leave gt rse ym Users gt mi Alis imaging amp Amanda gt mia 100416 gt mi 100514 gt ma 100611 gt 100714 24 03 14 In the Browse menu you can rename your images N Type a new name in in Image name box and click anywhere outside the box 15 Go back to the Acquire menu to take the next picture When you have finished transfer all your data to the Z network drive PLEASE TIDY UP g Microscope Nosepiece Objective Magnification 3264x2448 Interlaced Large HQ Camera Exposure Camera Image Type Camera Capture Format CreationDate 24 August 2012105703 Path ImageSize 3264 x 2448 RealSize 1398 86 x 1049 14 pm File Size Kb Clean lenses throw away used tissue lens tissue dispose of old slides in the yellow sharps bin
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