Home

User Manual Picus electronic pipette

1. 600 1 00 6 00 120 2 50 3 00 12 channel 0 2 10 ul 10 0 90 0 09 5 1 50 0 075 1 4 00 0 04 5 120 ul 120 0 50 0 6 60 0 70 0 42 12 2 00 0 24 10 300 ul 300 0 50 1 5 150 0 70 1 05 30 2 00 0 6 50 1200 ul 1200 0 50 6 00 600 1 00 6 00 120 2 50 3 00 28 Random Impr ul 0 40 0 04 0 70 0 035 1 50 0 005 0 15 0 18 0 20 0 12 1 00 0 12 0 15 0 45 0 20 0 3 0 80 0 24 0 15 1 5 0 20 1 0 0 50 0 5 0 15 7 5 0 20 5 00 0 40 2 00 0 20 20 00 0 30 15 00 0 60 6 00 0 50 0 05 0 80 0 04 3 00 0 03 0 20 0 24 0 30 0 18 1 50 0 18 0 20 0 6 0 30 0 45 1 00 0 3 0 20 2 4 0 30 1 8 1 00 1 2 0 50 0 05 0 80 0 04 3 00 0 03 0 20 0 24 0 30 0 18 1 50 0 18 0 20 0 6 0 30 0 45 1 00 0 3 0 20 2 4 0 30 1 8 1 00 1 2 8 2 Single channel pipettes speed in seconds Speed 1 cO COI O oO B O IN Speed table Speed is measured in Pipetting mode with the maximum volume Speed can be adjusted in all pipetting modes individually for aspiration and dispensing Speed range is from 1 slow to 9 fast 10 ul 2 5 1 8 13 1 0 0 8 0 6 0 5 0 4 0 3 120 pl 6 0 4 2 2 9 EN 1 5 1 1 09 0 7 0 6 Multichannel pipettes speed in seconds Speed 1 cO OC JI O 01 amp O JN 10 ul 2 5 18 13 1 0 0 8 0 6 0 5 0 4 0 3 120 pl 6 1 4 4 22 2 4 1 8 1 4 1 1 0 9 0 7 300 ul 7 1 5 3 dui 2 7 19 1 4 0 9 0 8 300 ul 5 4 3 9 2 9 ZA 1 6 1 2 1 0 0 8 0 7 29 1000 ul 10 1 7 4
2. 5 4 3 8 2 8 1 9 2 0 8 0 6 1200 ul 6 1 4 4 3 3 2 5 1 9 1 4 11 0 9 0 7 5 ml 10 2 7 4 5 4 3 8 2 7 1 8 1 1 0 8 0 6 10 ml 10 2 7 4 5 4 3 8 2 9 2 2 152 1 3 0 9 9 Ordering detalls Cat No 735021 735041 735061 735081 735101 735111 735321 735341 735361 735391 735421 735441 735461 735491 All pipettes include universal AC adaptor EU UK US JPN AUS and CHN plugs Channels Volume Range ul 0 2 10 ul 5 120 yl 10 300 ul 6 50 1000 ul 100 5000 ul 500 10000 ul 0 2 10 ul 5 120 ul 10 300 ul 50 1200 ul 0 2 10 ul 5 120 pl 0 10 300 ul 50 1200 ul Safe Cone Filters Standard Plus 721008 721007 721006 721005 721005 721008 721007 721006 721008 721007 721006 721018 721017 721016 721015 721015 721018 721017 721016 721018 721017 721016 30 Optifit Standard Tips 10 200 350 350 1000 5000 10000 10 200 350 350 1200 10 200 350 350 1200 SafetySpace Filter Tips 10 120 300 1000 10 120 300 1200 10 120 300 1200 82 Sartorius CC 2 EC Declaration of Conformity I the undersigned hereby declare that the Product s Biohit Picus Electronic Pipette Conforms to the Essential Requirements listed in Annex I of Council Directive 98 79 EC IVD Directive the EMC requirements of Council Directive 2004 108 EEC EN 61326 and the safety requirements of Council Directive 2006 95 EEC EN 6101
3. Biohit property unless otherwise stated Patents granted or pending 32 Publication No SUL6001 e12021 e Order No 85032 542 12 e Ver 01 2012 490093EN Sartorius Biohit Liquid Handling Oy
4. Mixing ON Mixing OFF Mixing is currently turned on and pressing Mixing OFF will turn Mixing OFF When mixing is activated pipette will show the default volume for mixing 8096 of the dispensed volume Mixing volume can be adjusted by the user by turning the adjustment wheel and the settings can be confirmd by pressing the operating button mixing volume cannot exceed the pipette s maximum volume To change the mixing from manual to automated press the operating button Manual mixing hold the operating button pressed down Set the mixing times by turning the adjustment wheel and press the operating button to confirm the setting Automated mixing can be paused by pressing the operating button Press the operating button again to continue dispensing Press QUIT left soft key to stop mixing and empty the tip by pressing the operating button 4 Excess Adjustment Rev Pipetting Multi Dispensing Sequential Dispensing Excess Adjustment can be used to set the excess volume When activated the excess volume is set to default Turn the adjustment wheel to reset the excess volume and confirm the setting by pressing the operating button NOTE Default is the maximum excess volume and can only be decreased from default 5 Automated Dispensing Multi Dispensing Automated dispensing dispenses the liquid automatically without the need to press the operating button The delay for the dispensing can be set in time frame from 0 1 sec to 9 9
5. Shortwave ultraviolet 254 nm Power 2x20W twin tubes Distance from tube surface to table product surface 620 mm Tested UV dosage 33000 mJ cm 4 2 3 Disinfection Sartorius Biohit electronic pipettes can be cleaned and decontaminated using disinfection or decontamination liquids such as Biohit Proline Biocontrol decontamination fluid 70 ethanol 6090 isopropanol mild detergent or similar Always make sure of the chemical compatibility between the materials of the pipette and the disinfectant or decontamination liquid Single channel pipettes For complete decontamination of the parts tip ejector collar tipcone spring and piston can be placed into a dish containing Biohit Proline Biocontrol or similar Leave the parts to soak for 30 minutes Rinse the parts with distilled water and let dry before reassembling Multichannel pipettes Wipe the outer surface of the multichannel pipettes with Biohit Proline Biocontrol decontamination fluid 70 ethanol 6090 isopropanol and mild detergent or similar The lower part of the multichannel pipettes should be opened only by an authorised Sartorius service provider 22 4 3 Performance testing It is recommended that the performance of your Sartorius Biohit pipettes is checked regularly e g every 3 months and always after in house maintenance A regular testing routine should be established by the users for their pipettes having regard to the accuracy requirements of the applicat
6. bulk packaging and choose the purity level your application requires DNase RNase and endotoxin free and or pre sterilised n case you need to be aware of aerosol contamination you may choose between Safe Cone Filters which are attached to the tip cone and should changed daily or SafetySpace Filter Tips which are discarded after each pipetting Choose SafetySpace Filter Tips also when you want to avoid lost samples due to the sample reaching the filter The small extra space between the sample and the filter ensures that neither foaming nor viscous liquids nor any sample when you are using reverse pipetting or repetitive multiple dispensing mode of the electronic pipette will touch the filter Pre rinse the tip 3 5 times before pipetting especially important in forward pipetting mode After dispensing wipe the tip against the receiving vessel wall to retain the last droplet Change the tip after every pipetting zl CD UV E UN 1 3 Content of delivery package Picus is delivered ready for use in a box containing Picus electronic pipette Universal AC adaptor Biohit Optifit Tip rack 10x96 tips with Picus up to 1000 ul single channel models 1200 ul with multichannel models One tip with Picus 5 ml and 10 ml single channel models Safe Cone Filters and forceps with models 510 ul Autoclavable grease with single channel models OC certificate Quick Start Guide Tip insert card If any it
7. sec 6 Fast dispensing Titrate Fast volume dispensing dispenses the first volume rapidly After dispensing the fast volume dispensing is continued manually 3 3 10 Setup Setup is the menu for user settings for calibration adjustment sound and backlight user identification GLP info and software reset function 3 3 10 1 Calibration Adjustment Adjustment enables the user to adjust the pipette in 1 3 calibration points 1 point adjustment calibration in one point set the calibration volume 2 point adjustment calibration in 10 and 10096 of the nominal volume 3 point adjustment calibration in 10 50 and 100 of the nominal volume Adjustment is always done in P mode and the adjustment is valid in all modes when activated When the Adjustment is activated ADJ symbol is seen on display Select Menu Select Setup Select Adjustment Factory set the pipette to the default factory settings press the operating button or OR t is possible to store three 3 different adjustments in the adjustment memory ADJ1 ADJ2 ADJ3 Activate desired memory place by using the adjustment wheel and pressing the operating button he pipette asks to set the custom adjustment values Press the operating button or OK to activate the values Editing the adjustment values Use the adjustment wheel to select the desired memory place ADJ1 ADJ2 or ADJ3 and press EDIT Select the desired calibration 1 point 2 points or 3 p
8. setting by pressing the operating button Maximum excess volume is set as default and can only be decreased from default Automated dispensing dispenses the liquid automatically without the need to press the operating button Delay for the Dispensing can be set in timeframe 0 1 sec to 9 9 sec Tracker advises the user in microplate dispensing by displaying the location for the next dispensing NOTE Only one additional feature can be selected at the time 3 3 4 Manual pipetting Pipetting where the piston movement in aspiration and dispensing is controlled by manually turning the adjustment wheel Ideal in measuring reagent amounts and in applications in which the pipetting speed needs to be controlled manually Select manual in main menu Start pipetting by pressing the operating button or turning the adjustment wheel to aspirate the liquid Aspiration is controlled manually and is continued as long as the button is pressed or adjustment wheel is turned When the aspiration is done turn the adjustment wheel to change the direction of the dispensing When dispensing is done the pipette asks to empty the tip Press NO left soft key to start the aspiration again without blow out Pressing the operating button pipette will make blow out to empty the tip Editing the mode Edit the maximum aspiration volume Press EDIT in the middle of the display Parameter background colour is highlighted to in
9. settings Saves the selected programme to the memory location Alphabets symbols Active in memory settings and in setup settings for entering the letters numbers or symbols Right soft key ADV Active in main screen of each mode Activates the additional functions to be used in conjunction with main mode OK Confirms the selection CLEAR Active in memory settings to clear the entered name Hot key All Picus models include 10 memory places for the favourite and most used e pipetting settings e m e Storing the programme in the memory place V L 1 Programme the pipetting mode and press the hot key List of memory WI P peret Activating the programme from the memory place l 2 3 3 2 Pipetting modes places opens Choose the desired memory location by turning the adjustment wheel Press SAVE Activate the name editor by pressing the operation button Turn the adjustment wheel to change the characters and press the operating button to confirm the selection Middle soft key changes the characters numbers symbols and operating button or OK in right soft key confirms the setting Move to the next character by turning the adjustment wheel Proceed as described above until all characters are set Save the programme name by pressing SAVE CLEAR Erases the character BACK Cancels the overwriting OK Confirms the selection Press the hot key and choose the programme by turning the adjustment
10. sign in bottom right hand corner of the display indicates the charging level of the battery When the battery charging level is low the indicator sign blinks LOW and the pipette needs to be placed on the charger to be charged NOTE Before connecting the AC adaptor to the electrical outlet make sure the voltage settings correspond to the local electrical outlet Use of incorrect power supplies may damage the device Only use the power supplies recommended by the manufacturer 2 2 Power up 1 Press ON OFF button Pipette will turn on 2 Press tip ejection button as advised on the display The pipette is now ready to be programmed and operated 3 While used and or charged pipette is in active mode all the processor functions are activated and the display backlight is on 4 1 minute after last activity The pipette will turn to a power saving mode and the backlight is dimmed The pipette returns to active mode when any of its buttons are pressed or the adjustment wheel is turned 5 10 minutes after last activity The backlight is turned off but the display is still visible The pipette turns on if the operating button or ON OFF button is pressed but does not complete the specific function of these buttons 6 60 minutes after last activity The pipette turns OFF To power up the pipette again press operating button or ON OFF button The pipette will turn on also when charged If the pipette is turned off by pressing ON OFF b
11. wheel Press the operating button or OK to activate saved programme The pipette is now ready for pipetting The memory place indicator is displayed next to the mode name Picus electronic pipette has eight 8 pipetting modes and five 5 mode M10 indicates that 300 ul pipetting has been related additions saved on memory place 10 Main mode Additional functions to be used in conjunction with the main mode available in all Picus Tracker Mixing Counter Excess Volume Auto Dispensing models Adjustment Timed Pipetting e e e Reverse Pipetting e e e Manual Pipetting e e e Multi Dispensing Diluting Sequential Dispensing Multi Aspiration S SISSSSSS Titrate 3 3 Programming 3 3 1 Pipetting forward pipetting Pipetting p aspirates the selected volume of liquid and dispenses it with the blow out function Recommended for aqueous liquids liquids containing small amounts of detergent or proteins and solvents Select Pipetting in main menu The Pipetting mode is activated with the latest settings Start pipetting by pressing the operating button Editing the mode Press EDIT button middle soft key or turn the adjustment wheel to the end Parameter background colour is highlighted to indicate that the editing mode has been activated Turn the adjustment wheel to set the volume Confirm the setting by pressing the operating button or OK Press NEXT middle soft key to move the cursor
12. 0 IEC 62133 Product performance according ISO 86555 2 This declaration is supported by EC quality system approval statement Annex IV ISO 9001 ISO 13485 Certificate No 108129 2011 AQ FIN FINAS issued by DNV on 20 December 2011 ISO 17025 Certificate No K041 issued by FINAS on 16 December 2011 ISO 14001 Certificate No 108133 201 1 AE HEL FINAS issued by DNV on 20 December 2011 Date 05 01 2012 Jussi Heini CEO Sartorius Biohit Liquid Handling Oy Laippatie 1 00880 Helsinki Finland R H jr Sartorius Biohit Liquid Handling Oy Head Office Laippatie 1 00880 HELSINKI FINLAND Tel 4358 9 773 861 Fax 358 9 773 86 200 Business ID F12441885 8 Internet site www biohit com Contact us infoG biohit com Factory in Finland Tietokatu 4 87400 KAJAANI FINLAND Tel 358 9 773 861 Fax 358 8 633 1400 31 Contact details Sartorius Biohit Liquid Handling Oy Laippatie 1 FI 00880 Helsinki Finland Phone 358 9 755 951 info Ebiohit com www biohit com Headquarter Sartorius Corporate Administration GmbH Weender Landstrasse 94 108 37075 Goettingen Germany Phone 49 551 308 0 Fax 49 551 308 3289 www sartorius com The status of the information Specifications and illustrations in this manual is indicated by the date given below Sartorius Biohit reserves the right to make changes to the technol ogy features specifications and design of the equipment without notice All trademarks are Sartorius
13. Aspirates the total volume as well as an excess volume then repetitively dispenses equal volumes of liquid Recommended in long pipetting series and microplate dispensing Select Multi Disp in menu The Multi Disp mode is activated with the latest settings Start pipetting by pressing the operating button Editing the mode Press EDIT button the middle soft key or turn the adjustment wheel to the end Parameter background colour is highlighted to indicate that the editing mode is activated Turn the adjustment wheel to set the volume and aliquot Press NEXT middle soft key to move the cursor to the next parameter to be edited Confirm the setting by pressing the operating button or OK Press BACK to return to the previous screen without saving the changes HINT Hold the operating button down to reaspirate the liquid without emptying the tip Or press NO in left soft key to continue pipetting without emptying the tip Additional features Timed Automated Dispensing Excess adjustment and Tracker can be used in conjunction with Multi Dispensing Press ADV the right soft key to activate extra features Turn the adjustment wheel to move to the desired feature and confirm the selection by pressing the operating button or OK Excess adjustment can be used to set the excess volume When activated the excess volume is set to default Turn the adjustment wheel to reset the excess volume and confirm the
14. Convert the recorded masses m to volumes v by multiplying the mass with the correction factor Z Z values in the table below V m Z 11 Calculate the mean volume V delivered V Vi 10 12 For conformity evaluation calculate the systematic error es of the measurement in ul e V V V selected test volume or in 9o e 100 V V V 13 For conformity evaluation calculate the the random error of the measurement as standard deviation s M n n number of measurement 10 n 1 or as coefficient of variation CV 100s V 14 Compare the systematic error inaccuracy and random error imprecision to the performance specification values of your own laboratory 23 Z values ul mg Temp C Air Pressure kPa 95 100 101 3 20 0 1 0028 1 0028 1 0029 20 5 1 0029 1 0029 1 0030 21 0 1 0030 1 0031 1 0031 21 5 1 0031 1 0032 1 0032 22 0 1 0032 1 0033 1 0033 22 5 1 0033 1 0034 1 0034 23 0 1 0034 1 0035 1 0035 235 1 0036 1 0036 1 0036 NOTE Systematic error is the difference between the dispenced volume and the selected test volume Random error is the scatter of the dispensed volumes around the mean of the dispensed volume ISO 8655 1 NOTE Sartorius Biohit specifications are achieved in strictly controlled conditions ISO 8655 1 Users should establish acceptable maximum permissible errors based on the field of use and the accuracy requirements placed on the pipette ISO 8655 1 4 4 Repl
15. OTHER THAN THE MANUFACTURER S ORIGINAL ONES In the event of warranty claims or if the pipette fails to function according to Specifications please contact your local Sartorius representative Sartorius Biohit Quality Assurance Procedure guarantees that the Sartorius Biohit pipette you have purchased is tested before delivery and is ready for use Each Sartorius Biohit electronic pipette is CE IVD marked fulfiling the requirements of the Essential Requirements listed in Annex of EC Directive 98 79 EC IVD Directive and to the protection requirements of Council Directive 2004 108 EC relating to Electromagnetic Compatibility EN61326 and Safety IEC 61010 1 ed 3 0 6 Disposal In compliance with European Directive WEEE 2002 96EC on waste and reduction of hazardous substances in electrical and electronic equipments this device must not be recycled as unsorted municipal waste Instead this device must be collected separately in accordance the local recycling regulations Batteries should be disposed of in accordance with local legal regulations Do not dispose of batteries with domestic waste 25 7 Troubleshooting 7 1 Hardware reset If the pipette is unresponsive it is possible to force a turning off and reset reset the hardware This function will not affect the saved settings e g adjust ment memory To turnoff an unresponsive pipette press simultaneously ON OFF button and soft key for few seconds Pipette will turn of
16. Sartorius service provider Please contact your nearest EK Sartorius service provider or distributor 4 2 Sterilising he Sartorius Biohit electronic pipettes can be sterilised by autoclaving UV or by using disinfection or decontamination liquids such as Biohit Proline Biocontrol decontamination fluid 70 ethanol 6090 isopropanol mild detergent or similar Always make sure of the chemical compatibility between the materials of the pipette and the disinfectant or decontamination liquid Always follow the autoclaving instructions below 4 2 1 Autoclaving Lower parts of Sartorius Biohit electronic pipettes are autoclavable excluding multichannel 1200 ul Please see the autoclaving symbol printed on the lower part of the multichannel pipettes to make sure the lower part is autoclavable Autoclaving instructions 1 Remove the Safe Cone Filter if attached 2 Unscrew the lower part by turning the connecting collar and the dispensing head counterclockwise until it comes off 3 Sterilise the part at 121 C 1 bar overpressure for 20 minutes 4 Let the parts cool and dry before reassembling NOTE Multichannel pipette s lower parts can be autoclaved as one unit or separately as individual parts 4 2 2 UV sterilising Sartorius Biohit electronic pipettes are manufactured using UV resistant materials The UV resistance of the products has been tested under continuous 21 UV light under the following test conditions Wave length
17. VAM TU LE LO I ae is i a i i i S i i E arnb 18 OSCAR RE E LINE NANE E a saaa Ei ii i iii i i 19 A1 CLEANING ANID MAINTENANCE cia instantani iii i Rn e 19 211 SIMON Eet Cl DIDEL S sai saaa i a i i i i O tems nm 19 A12 M ukichannet pipe UCS asa asi os i A i a a i i S a ERU ADU 21 CD EK E L LE 21 ANA NEU Ore S VA O ones menester system 21 A22 UV EL eoe a ai i is eee D a DEDE 25 EE 25 4 35 PERFORMANCE eebe 23 AA REPLACING IHE RAEE RM eR C 24 4 5 REPLACEMENT PAR S ostenten M 24 COS E E iii A T O i i a T i a ET 24 DN RN E ii iii i i ii i i i E RRR 2b G DS POSA M 9 JE d ROUBLE FOOTING son 26 a RDA E RESE Darei n ii i E E li i mate 26 7 2 TROUBLESHOOTING LL t E 26 Es JEAN AL E 27 Gals PERFORMANCE ON CIE SLO LS gai sia sonets mn SUI DA daa e IUUD PUn DIUI Ce DE 28 iie EE De BLE RC tt 29 9 ORDERING DETAILS os aa ai i i i a i i i I SS 20 10 DECLARA HON OF CONFORMI L is ania eu 31 1 Introduction Biohit Picus electronic pipette introduces the latest innovations in hand held air displacement liquid handling devices Together with laboratory workers ergonomists and dedicated R amp D Sartorius Biohit has been able to create tools for safe and comfortable yet versatile pipetting Picus is available in single channel and multichannel models 8 and 12 channels To
18. acing the battery It is recommended that the Picus battery is replaced only by authorised Sartorius service provider Please contact your nearest Sartorius representative 4 5 Replacement parts To replace broken or worn parts Sartorius Biohit offers a full range of spare parts Always contact the manufacturer or manufacturer s authorised representative for original spare parts for your Sartorius Biohit pipette 4 6 Storage When not in use it is recommended that the Biohit Picus electronic pipettes are stored in the charging unit During long storage periods several months it is recommended that the charging unit is disconnected from the power outlet and the pipette is turned off by pressing the power button on the top of the pipette 24 105 1 0029 1 0030 1 0031 1 0032 1 0033 1 0034 1 0036 1 0037 5 Warranty Sartorius Biohit products are meant to be used as described in this user manual Biohit Picus electronic pipettes are covered by a warranty for two years against workmanship and defects in materials excluding battery ANY WARRANTY WILL HOWEVER BE DEEMED AS VOID IF THE FAULT IS FOUND TO HAVE BEEN CAUSED BY MISTREATMENT MISUSE UNAUTHORIZED MAINTENANCE OR SERVICE OR NEGLIGENCE OF REGULAR MAINTENANCE AND SERVICE ACCIDENTAL DAMAGE INCORRECT STORAGE OR USE OF THE PRODUCTS FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS OUTSIDE THEIR SPECIFICATIONS CONTRARY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH TIPS
19. button The aspirated volume is shown on the display Editing the mode Press EDIT the middle soft key or turn the adjustment wheel to the end Parameter background colour is highlighted to indicate that the editing mode is activated Turn the adjustment wheel to set the numbers Confirm the setting by pressing the operating button or OK Press NEXT the middle soft key to move the cursor to the next parameter to be edited Press BACK to return to the previous screen without saving the changes Confirm the setting by pressing the operating button or OK Additional features Fast volume dispensing dispenses the first volume rapidly After dispensing the fast volume dispensing is continued manually 3 3 9 Additional functions used in conjunction with the main mode ADV in right soft key 1 Tracker Pipetting Rev Pipetting Multi Dispensing Tracker is a helpful feature for microplate dispensing and a unique feature only available in Picus Activate the Tracker in main modes ADV menu right soft key by using the adjustment wheel to scroll on the Tracker and press the operating button to activate the Tracker Tracker ON Tracker is currently OFF and pressing Tracker ON will turn Tracker ON Tracker OFF 2 Tracker is currently turned on and pressing Tracker OFF will turn Tracker OFF Single channel pipettes Select the microplate 96 or 384 wellplate and if the pipetting is done in rows or columns press the operating b
20. cter background turns white to indicate that it is being edited Available characters are alphabet in capital and lowercase letters numbers and symbols Use middle soft key to change the characters To select the alphabet number symbol turn the adjustment wheel Press the operating button or OK to save the choice Press the operating button to move the cursor to the next character and continue until the ID is completed Press SAVE the setting is saved Press BACK the setting is not changed NOTE Clearing the current ID is not necessary it can be overwritten 3 3 10 5 GLP Info GLP Good Laboratory Practice information indicates the date of the previous service calibration and when the next service calibration is due Select menu Select Setup Select GLP Info Turn the adjustment wheel to set the date of previous service and or calibration and press the operating button to move the cursor forward When the settings for the dates are set press the operating button or OK to save the settings Press BACK the settings are not changed NOTE Picus will not remind the user of the next service and or calibration 3 3 10 6 Reset Resetting the pipette will reset all the settings user has done to the factory settings including programming in the memory places Select menu Select Setup Select reset Pipette asks to set all the pipette settings to factory defaults Press the operating button or YES and tip ej
21. dicate that the editing mode is activated urn the adjustment wheel to set the volume Confirm the setting by pressing the operating button or OK Press NEXT the middle soft key to move the cursor to the next parameter to be edited Press BACK to return to the previous screen without saving the changes Confirm the setting by pressing the operation button or OK 3 3 5 Diluting Two different liquids separated by an air gap are aspirated and then dispensed simultaneously with automatic blow out Select diluting in main menu Start pipetting by pressing the operating button or by turning the adjustment wheel to aspirate the liquid 15 aspiration dilutor 2 aspiration air gap 3 aspiration sample Dispense by emptying the tip into the receiving vessel Editing the mode Press EDIT in the middle of the display Parameter background colour is highlighted to indicate that the editing mode has been activated urn the adjustment wheel to set the volume Confirm the setting by pressing the operating button or OK Press NEXT the middle soft key to move the cursor to the next parameter to be edited Press BACK to return to the previous screen without saving the changes Confirm the setting by pressing the operation button or OK Additional features Mixing can be used in conjunction with Diluting Press ADV the right soft key to activate advanced functions Mixing mixes the liquids manually or automatical
22. e AC adaptor for charging stand for 4 pipettes Input voltage Output voltage Picus electronic pipette Operating temperature Air humidity Weight single channel 10 300 ul Weight single channel 50 1000 ul Weight 8 channel 10 300 ul Length single channel 10 300 ul Length single channel 50 1000 ul Length 8 channel 10 300 ul Pipetting force for single and multichannel models Tip ejecting force for single and multichannel models Li Polymer battery with protection circuit 3 7 V 350 mAh Approx 1 hour For Indoor and Office Equipment Use Only 100 240V 50 60Hz 180mA 5V 1A LPS According to local requirements 7 5 VDC 300 mA According to local requirements 9 VDC 1200 mA 15 C to 40 C Max 80 100 g 110g 160 g 21 0 cm 21 6 cm 21 6 cm 1 3 N 3 1 N 21 8 1 Performance specifications 1 channel Increment Test volume Systematic Inacc ul ul ul 0 2 10 ul 0 02 10 0 90 0 09 5 1 00 0 05 1 2 50 0 025 5 120 ul 0 10 120 0 40 0 48 60 0 60 0 36 12 2 00 0 24 10 300 ul 0 20 300 0 40 1 2 150 0 60 0 9 30 1 50 0 45 50 1000 ul 1 00 1000 0 40 4 00 500 0 60 3 00 100 1 50 1 5 0 1 5 ml 5 00 5000 0 50 25 00 2500 0 80 20 00 500 1 00 5 00 0 5 10 ml 10 00 10000 0 60 60 00 5000 1 20 60 00 1000 3 00 30 00 8 channel 0 2 10 ul 10 0 90 0 09 5 1 50 0 075 1 4 00 0 04 5 120 ul 120 0 50 0 6 60 0 70 0 42 12 2 00 0 24 10 300 ul 300 0 50 1 5 150 0 70 1 05 30 2 00 0 6 50 1200 ul 1200 0 50 6 00
23. ection the pipette will reset the settings to the factory default Press BACK the settings are not changed 3 3 10 7 Information Software version and battery charging level are shown in information menu Information menu cannot be edited by the user 18 4 Care and maintenance Any precision instrument such as a pipette comprises a great variety of mechanical and electronic parts that are subject to wear and tear By taking care of your pipette and carrying out regular maintenance and calibration you can ensure the functionality and performance of the pipette To avoid contamination of the pipette and the sample and to extend pipette s lifetime use Biohit Safe Cone Filters available for pipettes 510 ul or Biohit SafetySpace Filter Tips Sartorius provides service maintenance and calibration services through its global network of service centres please contact your nearest service center for assistance Please note that warranty will be voided in the event that the pipette is disassembled or assembled by an unauthorised person 4 1 Cleaning and maintenance Sartorius Biohit electronic pipettes can be cleaned and decontaminated by using disinfection or decontamination liquids such as Biohit Proline Biocontrol decontamination fluid 70 ethanol 60 isopropanol mild detergent or similar Always make sure of the chemical compatibility between the materials of the pipette and the disinfectant or decontamination liquid Turn of
24. eel to set the volume Confirm the setting by pressing the operating button or OK Press NEXT middle soft key to move the cursor to the next parameter to be edited Press BACK to return to the previous screen without saving the changes Confirm the setting by pressing the operating button or OK Press MENU Choose mode Press EDIT Change settings and press OK or NEXT HINT Hold the START button down to reaspirate the liquid without emptying the tip Or press NO left soft key to continue pipetting without emptying the tip Additional features Counter Excess adjustment and Tracker can be used in conjunction with Reverse Pipetting Press ADV the right soft key to activate advanced functions Turn the adjustment wheel to move to the desired function and confirm the selection by pressing the operating button or OK Counter counts the number of dispensings Counting can be selected to start in any wanted number Excess adjustment can be used to set the excess volume When activated the excess volume is set to default Turn the adjustment wheel to reset the excess volume and confirm the setting by pressing the operating button Default is the maximum excess volume and can only be decreased from default Tracker advises the user in microplate dispensing by displaying the location for the next dispensing NOTE Only one additional feature can be selected at a time 3 3 3 Multi Dispensing
25. em in this delivery package is missing or damaged please contact your local Sartorius representative 2 Getting started Please read this manual before using Picus electronic pipette 1 Picus is delivered ready for use battery placed on 2 Place the replaceable Safe Cone Filter s into the tip cone s before use to avoid contaminations Charge the pipette before first use for one 1 hour Press the ON OFF button on top of the pipette to turn the power on Press tip ejector as advised on display Pipette is now ready to be programmed and operated BD gi ee 2 1 Charging It is recommended that you charge the pipette before first use for one 1 hour Place the pipette onto the charging stand or connect to the AC adaptor Make sure that the charging stand is connected to the power outlet and that the charging contacts of the pipette are properly attached to the charging tracks of the charging stand Charging with AC adaptor Connect Picus to the USB cable and plug in the power supply Charging in charging stand or carousel Make sure the charging stand is connected to the power outlet and the charging contacts of the pipette are properly attached to the charging tracks of the charging stand Charging options for Sartorius Biohit electronic pipettes AC adaptor universal included in the package Biohit charging stand for one pipette cat no 730981 Biohit charging carousel for 4 pipettes cat no 730991 Battery
26. f and can then be turned on again by pressing ON OFF button NOTE Make sure the pipette is not placed on the charging unit or connected to the USB cable while being reset 7 2 Troubleshooting guide Problem Possible Cause Solution Leakage Incomaptible tip Use original Sartorius Biohit tips Tip is loose Attach the tip firmly Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service Inaccuracy Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service Pipette does not turn on Pipette is in OFF mode Turn the pipette ON by pressing ON OFF button Empty battery Connect the pipette to the charger Piston is jammed Inner lower parts are loose Open the lower part and tighten the parts Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service Pipette does not aspirate Safe Cone Filter is dirty Replace the Safe Cone Filter liquid properly Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service 26 8 Technical data Rechargeable battery Type Capacity Charging time AC adaptors charging stands AC adaptor for Picus pipette Input voltage Output voltage AC adaptor for charging stand for 1 pipette Input voltage Output voltag
27. f the pipette prior to cleaning It is recommended always to use gloves when cleaning the pipette Change the Safe Cone Filter regularly with the forceps provided in the pipette package Cleaning is recommended always after possible contamination or dispensing corrosive or other aggressive chemicals Never let liquids enter the inner parts of the pipette Never use the pipette without tip attached to the tip cone Tools needed lint free cloth cleaning solution Biohit Proline Biocontrol 4 1 1 Single channel pipettes All models Outer surface Clean any visible dirt with mild detergent or similar and lint free cloth and wipe dry Tip ejector collar tip cone and piston Turn the pipette OFF Remove the Safe Cone Filter if attached Models 10 ul and 120 ul Unscrew the tip ejector collar counter clockwise and remove it Unscrew the tip cone locking ring counter clockwise and carefully remove it Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with cleaning solution or similar and lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not overtighten Put a thin layer of autoclavable grease cat no 731141 on the piston Carefully place the tip cone and attach it by screwing the tip cone locking ring clockwise Place the Safe Cone Filter with forceps Safe Cone Filter in
28. gether with Optiload tip loading mechanism and electronic tip ejection tip ejection is safe and comfortable To improve safety by avoiding contaminations in pipetting work all Picus models 10 yl feature replaceable Safe Cone Filters In Picus electronic pipette ergonomics is brought to a completely new level with extremely lightweight small size and comfortable operation 1 1 Intended use Picus electronic pipette is intended to be used in liquid handling applications for dispensing liquids in a wide volume range from 0 2 ul to 10 ml It is recommended that the Biohit Optifit Tips and SafetySpace Filter Tips are used to ensure the best compatibility and performance This liquid handling device is designed and manufactured to be used with IVD products as an IVD accessory and as stand alone laboratory equipment reddot design award winner 2012 fenniaprize 12 good design grows global HONORARY MENTION 1 2 Product overview Before using Biohit Picus electronic pipette for the first time please review this operating manual carefully This manual can be downloaded from Sartorius Biohit website www biohit com liquid handling manuals or ordered from your local Sartorius representative 1 2 1 Single and multichannel pipettes 2gouiopnsecesmo _ pe pe De USB charging socket ON OFF button Charging contacts Operating button Adjustment wheel Electronic tip ejector Hot key for memory places Soft ke
29. ion frequency of use number of operators using the pipette nature of the liquid dispensed and the acceptable maximum permissible errors established by the user ISO 8655 1 Performance tests should take place in a draught free room at 15 30 C constant to 0 5 C and relative humidity above 50 The pipette tips and the test water should have stood in the test room for long enough at least 2 hours to reach equilibrium with the room conditions Use distilled or de ionised water ISO 3696 grade 3 Use an analytical balance with a readability according ISO 8655 6 Weighting 1 Adjust the desired test volume V 2 Carefully fit the tip onto the tip cone 3 Fil the tip with test water and expel to waste five times to reach humidity equillibrium in the dead air volume 4 Replace the tip Pre wet the tip by filling it once with the test water and expell to waste 5 Aspirate the test water immersing the tip only 2 3 mm below the surface of the water Keep the pipette vertical 6 Withdraw the pipette vertically and touch the tip against the side wall of the test water container 7 Pipette the water into the weighting vessel touching the tip against the inside wall of the vessel just above the liquid surface at the angle of 30 to 45 Withdraw the pipette by drawing one tip 8 10 mm along the inner wall of the weighing vessel 8 Read the weight in mgs m 9 Repeat the tet cycle until 10 measurements have been recorded 10
30. is sartorius BJ Picus electronic pipette User Manual able of contents UR Ne RUMP E 3 UNE SITE AE c moment a i i 3 EM ue p UNUS T a a R S 4 DNS OE OU CCD REL PIP SL LOS vies aset a ii a a aaa 4 TAA DEDI 5 TD Beg KC TUC LO LE Ei i i i i ii i p a a i ia B 1 2 4 Biohit Optifit Tips and SafetySpace Filter TIpS aannananavnann gt nzr r vnnn nnnnnnnnnnnnnnnnnnannnnannnnannnnnaanunanannaanananann 5 1 3 CONTENT OF DELIVERY PACK AE asi issen niviriiasus ban ki iii bens sants imants 6 VG SENI EE 57 D Lana iii asis nt esmen i i O i i n 7 y Me EC 7 PM EI dU abi i r i i E T a ri i na 7 E CN UE E anem t a E i ny tit it 8 3 1 PRINCIPLE OF bus lo i a i i a a nana 8 SZ e Kl a ii i ii a i a a UU 9 J PROGRAMMING EE 10 EPD EE iai a a eentaents 10 GR I e WEE 10 HE DS DES MO asi ia i e i i i i E i i a a i MN UUE 11 s NO M i LIU E 12 ER EE Le DE 12 3 3 5 Sequential DISPENSING T 13 ANIOL e en UNUM NU NEED I IET fU NU 13 J EN CIA LE oie a a tree D aa OIN DU EU UND DIDI dicent 14 99 9 AAOOEIOITSE EE 15 Cu P 16 SENI ON BESTE CEI mI 16 e ut En E 17 CO LD SCI e a a a a UM I a 17 Sm gle S O nt 18 SE E DLP EO i i i aj i i i i a i e 18 535 OGC SE M a ai ii i i p Da 18 S NO
31. ly Ihe mixing volume can be adjusted according to the pipette s maximum volume 3 3 6 Sequential Dispensing Dispenses repetitively different volumes in any desired order Useful mode for example dilution series and in making the calibration curves Select Seq Disp in menu Seq Disp mode is activated with the latest settings Aspirate the liquid into the tip and dispense in set aliquots After last dispensing double click operating button to empty the tip or press NO to continue dispensing without emptying the tip Editing the mode Press EDIT the middle soft key or turn the adjustment wheel to the end Parameter background colour is highlighted to indicate that the editing mode has been activated Turn the adjustment wheel to set the numbers Confirm the setting by pressing the operating button or OK Press NEXT the middle soft key to move the cursor to the next parameter to be edited Press BACK to return to the previous screen without saving the changes Confirm the setting by pressing the operating button or OK Additional features Excess adjustment can be used in conjunction with Sequential dispensing Press ADV the right soft key to activate extra features Excess adjustment can be used to set the excess volume When activated the excess volume is set to default Turn the adjustment wheel to reset the excess volume and confirm the setting by pressing the operating button Maximum excess vol
32. models gt 10 ul urn the pipette ON Models 300 ul and 1000 ul Unscrew the tip ejector collar counter clockwise and remove it Unscrew the tip cone locking ring counter clockwise and carefully remove it Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with cleaning solution or similar and lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not overtighten Put a thin layer of autoclavable grease cat no 731141 around the seal Carefully place the tip cone on the piston attach it by screwing the tip cone locking ring clockwise Attach the tip ejector collar by screwing it clockwise Place the Safe Cone Filter with forceps Turn the pipette ON Model 5 ml Unscrew the tip ejector collar counter clockwise and remove it Unscrew the tip cone counter clockwise and remove it Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with mild detergent or similar and lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not over tighten Put a thin layer of autoclavable grease cat no 731141 on the seal and the interior of the tip cone avoid excess grease Carefully place the tip cone on the piston and screw clockwise Attach the
33. oints First display shows the target volume press OK Display shows the actual volume Enter the volume by turning adjustment wheel and press operating button or OK Pipette will ask One 1 volume in 1 point adjustment Two 2 volumes in 2 point adjustment Three 3 volumes in 3 point adjustment 3 3 10 2 Sound The sounds can be adjusted for the adjustment wheel and messages errors and notifications It is also possible to turn all the sounds OFF Select menu Select Setup Select sound Use the adjustment wheel to set the sounds ON OFF Press the operating button or OK to save the settings Press BACK the settings are not changed 3 3 10 3 Backlight Display colours equal to the colour coding of the display cover and operating button colours Colours are selected to indicate the tip compatibility Colour coding is active during charging while pipetting the colour coding is off and the display is white to maximise the contrast Select menu Select Setup Select backlight Use the adjustment wheel to set the backlight If the display colour is changed the display is always set to the selected colour and the colour will not change to optimal contrast while pipetting 3 3 10 4 User ID This liquid handling device may be personalised by the user Personalising on software Select menu Select User ID Press CLEAR to clear the current ID or character Press the operating button first chara
34. tip ejector collar by screwing it clockwise Place the Safe Cone Filter with forceps Turn the pipette ON Model 10 ml Unscrew the tip ejector collar counter clockwise and remove it 20 Hold from tip cone with your finger and open bayonet locking by turning bayonet locking ring clockwise until it stops remove tip cone Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with cleaning solution or similar and lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not overtighten Put a thin layer of autoclavable grease cat no 731141 on the seal and the interior of the tip cone avoid excess grease Carefully place the tip cone on the piston hold the tip cone and turn bayonet locking ring counterclockwise until it stops to lock the tip cone Make sure that the tip cone is properly tightened do not overtighten Attach the tip ejector collar by screwing it clockwise Place the Safe Cone Filter with forceps urn the pipette ON NOTE It is always necessary to check the performance of the pipette after cleaning or maintenance 4 1 2 Multichannel pipettes Outer surface Clean any visible dirt with cleaning solution or similar and lint free cloth and wipe dry Lower part Opening of the lower part of the multichannel pipettes should always be 121 C done by an authorised
35. to the next parameter to be edited Press BACK to return to the previous screen without saving the changes Confirm the setting by pressing the operating button or OK Additional functions Counter Mixing and Tracker can be used in conjunction with the Pipetting mode Press ADV in the right soft key to activate advanced functions urn the adjustment wheel to move the cursor to the desired function and confirm the selection by pressing the operating button or OK Counter counts the number of dispensings Counting can be selected to start in any desired number Mixing mixes the liquids manually or automatically the mixing volume can be adjusted according to the pipette s maximum volume Tracker advises user in microplate dispensing by displaying the location for the next dispensing NOTE Only one additional feature can be selected at a time 3 3 2 Reverse Pipetting Aspirates the selected volume as well as an excess volume Recommended for biological foaming and viscous liquids In dispensing mode the excess is left inside the tip and is then discarded Select Reverse mode in main menu The Reverse mode is activated with the latest settings Start pipetting by pressing the operating button Editing the mode Press EDIT button the middle soft key or turn the adjustment wheel to the end Parameter background colour is highlighted to indicate that edit mode has been activated Turn the adjustment wh
36. ume is set as default and can be only decreased from default 3 3 7 Multi Aspirating Select the volume and the number of aspirations desired Repeat the aspiration until the series is completed then discard the full aspirated volume in a single step Multi Aspirating is a useful mode for example for sample pooling and microplate washing Select Multi Aspirating in menu Multi Asp mode is activated with the latest settings Start pipetting by pressing operating button Pipette aspirates the volumes set by the user in any desired order to the tip Dispense the liquid from the tip to the receiving vessel Editing the mode Press EDIT the middle soft key or turn the adjustment wheel to the end Parameter background colour is highlighted to indicate that the editing mode is activated Turn adjustment wheel to set the numbers Confirm the setting by pressing operating button or OK NEXT in the middle soft key moves the cursor to the next parameter to be edited BACK returns to the previous screen without saving the changes Confirm the setting by pressing the operating button or OK NOTE No additional functions are available for Multi Aspirating 3 3 8 Titrate Aspirates the selected volume automatically Dispensing is manually controlled by the user Select Titrate in menu The Titrate mode is activated with the latest settings Start pipetting by turning the adjustment wheel or by pressing the operating
37. utton it can be turned on by pressing the same button 3 Operation 3 1 Principle of operation Picus is an electronic pipette operated by the air displacement principle The unique DC motor electronic brake and piston control system inside the pipette complete with electronic tip ejection guarantees excellent accuracy precision and safety The unique adjustment wheel enables fast and comfortable operating and its location makes Picus yet several times more ergonomic and versatile than amechanical pipette due to its fully electronic function Pipetting functions are controlled with operating button adjustment wheel and soft keys Operating button Used to confirm the settings optional to soft key ok and moves the piston in aspiration and dispensing Adjustment wheel for fast volume setting Used for scrolling the menu and number settings Moves the piston in manual and titrating modes for aspiration and dispensing Unlocks the edit menu turn the adjustment wheel to the end to unlock the pipette for editing in active mode Electronic tip ejector Ejects the tip s with a light touch Soft keys MENU Displays the mode selection BACK Returns to the previous screen without saving the changes QUIT Quits the pipetting task Middle soft key EDIT Activates the changes to adjusted settings NEXT Active in edit mode Moves the cursor to the next parameter to be edited SAVE Active in memory
38. utton or OK to activate the Tracker First dispensing is shown on display Rows A1 A2 A3 B1 B2 B3 C1 C2 C3 Columns 1A 1B 1C 2A 2B 2C 3A 3B 3C The first dispensing location can be selected as desired 8 channel pipettes Select the microplate 96 or 384 wellplate Pipetting is possible in columns Press operating button or OK to activate the Tracker 96 well plate Columns 1 2 3 384 well plate Columns 1 Pipetting A1 C1 G1 2 Pipetting B1 D1 F1 3 Pipetting A2 C2 G2 4 Pipetting B2 D2 F2 The first dispensing column can be selected as desired 12 channel pipettes Select the microplate 96 or 384 well plate Pipetting is possible in rows Press operating button or OK to activate the Tracker 96 well plate Rows A B C 384 well plate Rows 1 Pipetting A1 A3 A5 2 Pipetting A2 A4 A6 3 Pipetting B1 B3 B5 4 Pipetting B2 B4 B6 The first dispensing row can be selected as desired 2 Counter Pipetting Rev Pipetting Cycle counter counts the pipetting cycles up to 99 It is possible to start counting from any desired number up to 99 Counter ON Counter is currently OFF and pressing Counter ON will turn Counter ON Counter OFF 2 Counter is currently turned on and pressing Counter OFF will turn Counter OFF 3 Mixing Pipetting Diluting Mixing ON Mixing is currently OFF and pressing Mixing ON will turn
39. ys for programming Dot matrix display Volume range and colour coding Dispensing head tip ejector collar and tip cone autoclavable excl 8 12 ch 1200 ul Optiload spring loaded tip cones in multichannel pipettes Safe Cone Filters excl 10 ul pipettes Pipette tip NI Ms 11 12 13 14 1 2 2 Display The multicolour backlit dot matrix display is clear and the structure is informative Soft key functions Current mode Pipetting volume and aliquots in multidispensing modes Advanced ADV mode if selected Speeds Battery charging indicator Arrows indicating aspiration and dispensing ND MIge ue ie 1 2 3 Soft key functions 1 Left soft key MENU BACK QUIT Middle soft key EDIT NEXT SAVE Alphabets symbols Right soft key ADV OK CLEAR Ge eo 1 2 4 Biohit Optifit Tips and SafetySpace Filter Tips It is recommended that Optifit Tips or SafetySpace Filter Tips are used with Sartorius Biohit pipettes This ensures the best possible compatibility and thus best possible accuracy and precision as the tip is designed for the pipette in question Additional pipetting instructions to achieve good results are as follows The liquid and the pipette tip combination should be approximately at the same temperature Choose the right tip volume for your pipette the colour of the tip tray should match the colour coding on the pipette Use Optifit Tips in rack refill or

User Manual Picus electronic pipette

Contents

Download Pdf Manuals

Related Search

Related Contents