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Nucleic Acid Isolation System QuickGene

1. Fuji Photo Film Co Ltd RRA Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd
2. Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd 2005 QuickGene 810 User s Guide Rev 1 3 ii QuickGene 810 QuickGene 810 ET gt Er s EE hh AN WARNING WARNING A BIOHAZARD BIOHAZARD Ac ON CAUTION AUTION CAU
3. Parts to clean Cleaning methods Pressure Head Wipe with a Kimwipe or papertissue soaked with ethanol QuickGene 810 outside Remove the dust lightly with a dry soft cloth QuickGene 810 inside Wipe with a Kimwipe or paper tissue soaked with 0 5 sodium hypochlorite solution Remove the dust lightly with a dry soft cloth QuickGene 810 User s Guide rev 1 3 A Maintenance 4 4 Cleaning the Parts The following table describes how to clean the parts Parts Cleaning methods Holder Carriage Cartridge Holder Clean the parts using one of the following methods 1 Clean with neutral detergent and then wash out with distilled water Tube Holder eu E Buffer Stand Next dip into 3 hydrogen peroxide solution at room temperature for 10 minutes Adapter Lastly wash the solution out with DEPC water and dry the parts 2 Soak in a 0 1N sodium hydroxide 0 1 EDTA solution overnight and then wash the solution out with DEPC water and dry the Holder Stand parts 3 Clean the parts with neutral detergent then wash out with 0 5 sodium hypochlorite solution and dry the parts Discharge Tra After using the QuickGene 810 dispose of the liquid collected in the 9 y Discharge Tray and wash the tray with water Pump Tube Wipe with a
4. A Fuji Photo Films Co Ltd QuickGene 810 Fuji Photo Films Co Ltd QuickGene 810 User s Guide Rev 1 3 Xi AS Qui 810 WARNING QuickGene 810 QuickGene 810 s i gt a i F Nez FM N cAUTION QuickGene 810 QuickGene 810 QuickGene 810 QuickGene 810
5. No Name Function Displays messages 1 LCD For the displayed messages refer to 2 4 LCD Display Information page 17 and 6 3 Error Messages page 46 Lamp LED The green light is on when the power is on 2 POWER The light is off in me following cases When the power is off When an abnormality is detected in the power unit 3 READY The green light is on when the QuickGene 810 is ready for operation The light is off during extraction process or setup The green light is on when the START button is pressed The green light blinks during binding process or when extraction process is 4 BINDING completed The orange light blinks when an error is detected The light is off in other cases The green light is on during binding process starting from when the START button is pressed The green light blinks during washing process or when extraction process is 5 WASHING en j The orange light blinks when an error is detected The light is off in other cases The green light is on during washing process starting from when the START button is pressed 6 ELUTION nn blinks during collection process or when the extraction process is The orange light blinks when an error is detected The light is off in other cases Button Note The buttons may only be used when the cover is closed Starts a program 7 START The operation cannot be started while the front cover is open or if an error has occurred I
6. QuickGene 810 QuickGene 810 QuickGene 810 QuickGene 810 A caution QuickGene 810 QuickGene 810 QuickGene 810 QuickGene 810 Kimwipe QuickGene 810
7. QuickGene 810 20 QuickGene 810 AC100 10 AC240 10 V 50Hz 60Hz 15 C 30 C 30 80 SESE QuickGene 810 21 FR ARM QuickGene 810 QuickGene 810 Fuji Photo Film Co Ltd QuickGene 810 QuickGene
8. QuickGene 810 xii J QuickGene 810 QuickGene 810 User s Guide Rev 1 3 Informations concernant la securite Ce chapitre d crit les pr cautions de s curit Pour votre s curit et celle des autres veuillez suivre les indications et recommandations fournies dans les pages suivantes et qui concernent l utilisation du QuickGene 810 A propos des instruments Saas AN Si les recommandations suivantes ne sont pas prises en compte un WARNING incendie ou une d charge lectrique peuvent se produire Utilisez un c ble d alimentation conforme aux r glementations de votre pays ou contactez votre revendeur local Connectez une prise lectrique mise la terre Ne touchez pas la fiche si vous avez les mains mouill es Nvutilisez pas le QuickGene 810 avec un voltage diff rent de celui qui est sp cifi sur l appareil Ne branchez pas trop appareils sur la prise lectrique Nvutilisez pas QuickGene 810 si la fiche est endommag e ou si la prise n est pas solidement fix e Siles broches de la prise ou de la fiche sont poussi reuses nettoyez les avec un chiffon sec Lorsque vous retirez la fiche de la prise faites le en tenant fermement la prise Ne tirez pas le
9. ey O Secure the pump cover in place with the lt original 2 screws Important eCheck the Pump Cover is fixed well to the Rear Then tighten the screws until the spring washer is squeezed Spring washer QuickGene 810 User s Guide Rev 1 3 11 1 Installation 6 Connect the tube on the device side to the Pump Tube as the figure Connect the upper tube to the Wash Buffer Pump and connect the lower tube to the Elution Buffer Pump by turning as shown in the figure YY N NOS EN To Elution Buffer Pump Mm Setup the Buffer Stand The following explains how to install the Buffer Stand 1 Attach the Buffer Stand to the QuickGene 810 Insert the tabs of the Buffer Stand in the slits on the QuickGene 810 Buffer Stand 2 Set empty conical tubes into the Buffer Stand Discharge Tray Set two conical tubes for elution buffer and wash buffer When using standard conical tubes attach the adapter to the Buffer Stand Conical Tube A 3 Set the Discharge Tray to the Buffer Stand Adapter When setting standard conical tubes Buffer Stand Note When the carrier is on the left and it is difficult to attach the Buffer Stand close the front cover and press the CR MOVE button Then the carrier moves to the right end 12 QuickGene 810 User s Guide Rev 1 3 1 Installation m Setup the Tube Cap The following explains
10. 5 How to Set Parameters 38 The following table shows the meaning of each parameter Display LCD message Parameter name Meaning sequence Sets the peak pressure during pressure application Judgment criteria is the pressure before pressure application the BIND PEAK 50 Peak pressure on the pressure set in this item Pressure application may be 1 BIND PEAK 160 sample stopped before reaching the peak pressure if air pressure drops suddenly indicating that all liquid has been discharged Can be specified between 50 160 kpa in increments of 1kpa WASH COUNT 0 Frequency of wash Sets the frequency of wash buffer discharges WASH COUNT 5 buffer discharges Can be specified between 0 5 times Sets the peak pressure during pressure application Judgment criteria is the pressure before pressure application the WASH PEAK 50 Wash buffer peak pressure set in this item Pressure application may be 3 WASH PEAK 160 pressure stopped before reaching the peak pressure if air pressure drops suddenly indicating that all liquid has been discharged Can be specified between 50 160 kpa in increments of 1kpa 4 WASH VOL1 250 1st Wash Buffer Sets the first wash buffer discharge rate WASH VOL1 750 Discharge Rate Can be specified between 250 750yl in increments of 501 7 Sets the second wash buffer discharge rate 5 WASH VOL2 250 ond WBDR A g A WASH VOL
11. 3 Turn off the power Press the O side of the switch 4 Open the front cover Hold the handle and open the front cover until the upper limit is reached The front cover will not stay A CAUTION on the way to the upper limit If you release the front cover while opening it it may cause injury or device damage 5 Take off both the tube on the device side and the Tube Cap from the Pump Tube Take off both tube caps purple and blue by turning the connection points as shown in the figure 6 Take off the Buffer Stand from the QuickGene 810 7 Detach the pump cover Loosen and remove the screws to detach the pump cover 34 QuickGene 810 User s Guide rev 1 3 A Maintenance 8 Take off the Pump Tube from the pump and attach a new Pump Tube to the rear of the Pump Cover Pump Cover Rear Pump Cover Front Slide the connectors at the ends of the Pump Tube into the grooves of the Pump Cover Check that the notches on both connectors are facing the front of the Pump Cover Slide the Pump Tube into place inside the Pump Cover by rotating the rotor Adjust the rotor to its original vertical position Use the Pump Tube as labeled ELUTION Pump Tube or WASH Pump Tube for each pump Bump Tube Spare pump tube is available for either pump Connector Groove Important Slide the Pump Tube Notches face front Check the both end of the Pump
12. 750 HI 750 500 500 750 750 750 750 37 WAS2 VOL3 250 750 HI 750 500 500 750 750 750 750 QuickGene 810 User s Guide Rev 1 3 41 5 How to Set Parameters Mode name The value displays the default a ee ee BLOOD PLUS PLUS 38 WAS2 VOL4 250 750 HI 750 500 500 750 750 750 750 39 WAS2 VOL5 250 750 HI 750 500 500 750 750 750 750 40 ELUT VOL 50 400 HI 200 100 100 200 100 100 50 41 ELUT COUNT 0 5 time s 1 1 1 1 1 1 1 42 ELUT SPEED 1 12 7 7 7 7 7 7 7 43 ELUT PEAK 50 160 kpa 100 100 100 100 100 100 100 44 ELUTUPTIME 1 50 sec 4 4 4 4 4 4 4 45 ELUT RETRY 50 160 kpa 140 140 140 140 140 140 140 46 ELUTLOWER 3 100 kpa 65 65 65 65 65 65 65 47 ELUT DOWN TM 1 99 sec 15 15 15 15 15 15 15 48 ELUTRDNT 1 100 sec 50 50 50 50 50 50 50 49 ELUT FALL 5 60 kpa 50 50 50 50 50 50 50 50 ELUT DIP TM 0 510 kpa 0 30 30 90 30 30 0 51 W DSVOL S Bus ul 3000 3000 3000 3000 3000 3000 3000 52 EDSVOL S pee ul 3000 3000 3000 3000 3000 3000 3000 53 BUZZER pang ON ON ON ON ON ON ON 54 DETECT PRES 1 50 kpa 15 15 15 15 15 15 15 55 DOWN PRES 10 50 kpa 50 50 50 50 50 50 50 m EXPERT mode EXPERT mode is used to set more detailed parameters than in the normal setup mode The following explains how to shift to the EXPERT mode 1 Select the extraction mode whose parameter is to be changed Press
13. Press the DISCHARGE button on the operation panel 3 3 Preparation of Lysate Samples Prepare lysate samples Make lysate samples using the specified kit for each sample For details refer to the kit s handbook 3 4 Extraction Operations This section explains each operation before the start of a program and after the completion of a program m Operations before the start Important Perform the extraction operation according to the following procedures QuickGene 810 User s Guide Rev 1 3 21 3 Operation 22 1 Open the front cover 2 Take the Holder Carriage out of the QuickGene 810 Grip the handles lift the Holder Carriage up and then take it out Note When the carrier is in the center and it is difficult to remove the Holder N Carriage close the front cover and Grip handles and press the CR MOVE button lift to take out 3 Take the Tube Holder out of the Holder Carriage 4 Set collection tubes and waste tubes in the Tube Holder Important Put the tubes into the Tube Holder from the left without space 5 Set the Tube Holder into the Holder Carriage Align the tab and notch Align the tab and notch QuickGene 810 User s Guide Rev 1 3 3 Operation 6 Set cartridges into the Cartridge Holder Important Insert the cartridges into the cartridge holder to the bottom of the holes e Set cartridges in the order
14. 1kpa WAS2 VOL1 250 1st WBDR in the 2nd Sets the first wash buffer discharge rate in the second round 13 WAS2 VOL1 750 round Can be specified between 250 750ul in increments of 501 Sets the second wash buffer discharge rate in the second 14 WAS2 VOL2 250 2nd WBDR in the 2nd round WAS2 VOL2 750 round l Can be specified between 250 750ul in increments of 501 WAS2 VOL3 250 3rd WBDR in the 2nd Sets the third wash buffer discharge rate in the second round 15 WAS2 VOL3 750 round Can be specified between 250 750ul in increments of 501 WAS2 VOL4 250 4th WBDR in the 2nd Sets the fourth wash buffer discharge rate in the second round 16 WAS2 VOL4 750 round Can be specified between 250 750hl in increments of 501 WAS2 VOL5 250 5th WBDR in the 2nd Sets the fifth wash buffer discharge rate in the second round 17 m WAS2 VOLS 750 round Can be specified between 250 750hl in increments of 501 QuickGene 810 User s Guide Rev 1 3 5 How to Set Parameters Displey LCD message Parameter name Meaning sequence 18 ELUTVOL 50 elution buffer Sets the elution buffer discharge rate ELUT VOL 400 discharge rate Can be specified between 50 400I in increments of 501 Sets the peak pressure during pressure application Judgment criteria is the pressure before pressure application the ELUT PEAK 50 elution buffer peak pressure set in this item Pressure application may be 19 ELUT
15. Kimwipe or paper tissue soaked with ethanol Tube Cap Wipe with a Kimwipe or paper tissue soaked with ethanol 4 5 How to Replace the Pump Tube a This section explains how to replace the Pump Tube If the Pump Tube is continuously used without this maintenance the Pump Tube may break and the buffer may leak It is recommended that the Pump Tube be replaced before the required replacement date Note Ifthe QuickGene 810 will not be used for 1 year or more the Pump Tubes must be removed from the device Pump Tube for elution buffer The Pump Tube need to replace until about 50 000 extraction operations Pump Tube for wash buffer Replacing the Pump Tube is necessary approximately 3 years after the beginning of QuickGene 810 use or before performing 20 000 extraction operations Replace the Pump Tube with the spare one included in the package If all the tubes have been used purchase them separately as needed When purchasing them contact the Customer Center QuickGene 810 User s Guide Rev 1 3 33 A Maintenance m How to Replace the Pump Tube Perform the following procedures to replace the Pump Tube When replacing the Pump Tube wear gloves A CAUTION 1 Turn the power on Press the I side of the switch 2 Press the DISCHARGE button All the liquid in the tubes will be discharged In addition pressing the DISCHARGE button makes the pump rotate to the position for attaching the pump cover
16. PEAK 160 pressure stopped before reaching the peak pressure if air pressure drops suddenly indicating that all liquid has been discharged Can be specified between 50 160 kpa in increments of 1kpa i Sets the elution buffer immersion management time ELUT DIP TM 0 suton buffer 20 immersion Can be specified between 0 510 sec in increments of 2 ELUT DIP TM 510 management timer seconds 5 2 How to Set Change Parameters Settings of each parameter can be changed from the setup mode m How to change Parameters The following explains how to change settings of each parameter Example of messages dispalyed in operation panel SETUP START DNA WHOLE BLOOD il The present mode is displayed BIND PEAK 120 is the value saved in the memory QuickGene 810 User s Guide Rev 1 3 1 Select the extraction mode Press the MODE button several times until the target extraction mode appears 2 Press the A W buttons simultaneously When the LCD displays as shown in the figure the setup mode starts 3 Press the MODE button several times until the target parameter appears To return to the previous parameter press the DISCHARGE button 4 Change the parameter settings using the A V buttons 4 switch raises the setting value V switch lowers the setting value Holding one of the above switches down can change the setting values consecutively When c
17. Repair Service Period of Product 00 Rn 000 49 A 5 C stomer SAG Maan ne 49 A 6 Notes for Transfer QuickGene 810 50 QuickGene 810 User s Guide rev 1 3 INDEX 1 Installation This chapter explains how to install the QuickGene 810 2 Basic Functions This chapter explains the basic functions of the QuickGene 810 3 Operation This chapter explains the extraction procedures A Maintenance This chapter describes how to maintain the QuickGene 810 5 How to Set Parameters This chapter explains how to set parameters 6 Troubleshooting This chapter describes the procedures that should be taken when you suspect device failures and the messages shown on the LCD Appendix A This appendix describes after sales services and points to note for transfer QuickGene 810 User s Guide Rev 1 3 XXi xxii QuickGene 810 User s Guide Rev 1 3 1 Installation 1 Installation This chapter explains how to install the QuickGene 810 1 1 Installation and Use Conditions EN This section explains the installation conditions and operating environment of the QuickGene 810 m Installation conditions AN WARNING e Do not install the QuickGene 810 to a location where it can be splashed with water It may cause a device failure fire or electric shock e When relocating the QuickGene 81
18. Stromversorgung Betriebsumgebung etc nicht entsprechen Fuji Photo Film Co Ltd bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die auf Naturkatastrophen wie Feuer Erdbeben berschwemmung oder Blitzschlag zur ckzuf hren sind e Kein Teil dieser Anleitung darf ohne Erlaubnis der Fuji Photo Film Co Ltd reproduziert werden m Varenzeichen Firmen und Produktnamen sind Warenzeichen oder eingetragene Warenzeichen der jeweiligen Inhaber Alle Rechte vorbehalten Copyright 2005 Fuji Photo Film Co Ltd QuickGene 810 User s Guide Rev 1 3 vii viii Warnhinweise Diese Anleitung enth lt wichtige Informationen zur sicheren Verwendung des QuickGene 810 Lesen Sie diese Informationen sorgf ltig durch bevor Sie das QuickGene 810 in Betrieb nehmen Die Bedeutung der verschiedenen Sicherheitshinweise wird im Folgenden erl utert WARNING weist auf gef hrliche Betriebsbedingungen hin die zu JN WARNING ernsthaften Verletzungen oder Verletzungen mit Todesfolge f hren k nnen BIOHAZARD kennzeichnet die Gef hrdung durch biologische Agenzien oder Verh ltnisse die infekti s oder gesundheitssch dlich sein k nnen BIOHAZARD CAUTION weist auf gef hrliche Betriebsbedingungen hin die zu N CAUTION leichten bis mittleren Verletzungen f hren k nnen CAUTION kann dar ber hinaus das Risiko einer Sachbesch digung kennzeichnen Important Important kennzeichne
19. Tube are connector into groove placed the grooves Note Use the specified Pump Tube After this the procedure is the same as in How to install the Pump Tube For details refer to Il Setup the Pump Tube page 10 QuickGene 810 User s Guide Rev 1 3 35 4 Maintenance 36 QuickGene 810 User s Guide Rev 1 3 5 How to Set Parameters 5 How to Set Parameters This chapter explains how to set parameters Set Parameters means to change the values such as buffer volume number of discharges etc for each extraction mode The name of each extraction mode can be selected from 35 names The parameters set in the QuickGene 810 are stored in 7 separate memory areas Therefore even if an extraction mode name is changed the parameter values will not be changed 3 2 How to Set Change Parameters page 39 5 4 Changing Extraction Mode Names page 43 Important The setup is used to change the parameters according to new protocol of the kit etc Please check the parameters for each kit s Handbook In the case of wrong value for parameters it will cause buffer spill or sample extraction failure It is the user s responsibility to change the parameters In EXPERT mode there are some crucial parameters Therefore you must not change any parameters in EXPERT mode without our guidance 5 1 Parameters Settings of each parameter can be changed from the setup mode Some parameters are ch
20. be solved contact your local Customer Support If the QuickGene 810 has failed contact your local Customer Support A 4 Repair Service Period of Product The guaranteed repair service period of this product is seven years from the end of sales Thereafter repair services may not be provided if repair parts become out of stock Please note this A 5 Customer Support http lifescience fujifilm com Fuji Photo Film Co Ltd LIFE SCIENCE PRODUCTS DIVISION 26 30 Nishiazabu 2 Chome Minato ku TOKYO 106 8620 JAPAN Tel 81 3 3406 2201 Fax 81 3 3406 2158 E mail sginfo fujifilm co jp Subsidiaries lt United States Canada Mexico gt Fujifilm Medical System U S A Inc 419 West Avenue Stamford CT 06902 U S A Tel 1 203 324 2000 ext 6112 1 800 431 1850 ext 6112 in the U S Fax 1 203 351 4713 E mail SSG fujimed com URL http lifescience fujifilm com lt Europe excl UK and Ireland gt Fuji Photo Film Europe GmbH Heesenstr 31 40549 Dusseldorf Germany Tel 49 211 5089 174 Fax 49 211 5089 139 E mail lifescience fujifilmeurope de URL http www fujifilm de lt UK Ireland gt Fuji Photo Film U K Ltd Unit 12 St Martins way St Martins Business centre Bedford MK42 OLF U K Tel 44 1234 245291 Fax 44 1234 245293 E mail lifesciences fuji co uk URL http lifescience fujifilm com lt China gt Fuji Photo Film China Investment Co Ltd 31st floor Hong Kong New World Tower No 3
21. buffers wash buffer elution buffer are set here The buffers set are sent to the discharge unit through the pump unit Buffer setting unit Sends the buffers wash buffer elution buffer to the pressure unit according to Pump unit the extraction program 2 Puts pressurized air and buffers wash buffer elution buffer into the cartridge Carrier unit pean k and performs the extraction process binding washing elution p By moving the Tube Holder the waste liquid and extracted liquid are discharged Sample unit into the waste tube and collection tube respectively QuickGene 810 User s Guide Rev 1 3 15 2 Basic Functions 2 2 Working Process of QuickGene 810 The following describes the working flow of the QuickGene 8 10 1 The extraction program is started by using the operation unit 2 The wash buffer and elution buffer are sent from the buffer setting unit to the pump unit 3 The wash buffer and elution buffer are sent from the pump unit to the carrier unit 4 The pressurized air wash buffer and elution buffer are sent from the carrier unit into the cartridge and the extraction process is performed 5 The extracted sample is eluted to the collection tube set in the sample unit The waste is discharged to the waste tube 2 3 Safety Mechanism This section explains the safety mechanisms of the QuickGene 810 The QuickGene 810 provides the following safety mechanisms
22. cable Pour tout entretien d branchez la fiche de la prise murale Ne touchez pas la fiche lorsque le tonnerre gronde e Ne versez aucun liquide sur le QuickGene 810 Ne placez aucun objet contenant du liquide sur l appareil Vous risquez en le faisant de provoquer une panne un incendie ou une d charge lectrique e En cas de surchauffe de l appareil ou s il d gage de la fum e ou une odeur bizarre d branchez imm diatement le c ble d alimentation N caution e N essayez en aucun cas de remodeler ou modifier le QuickGene 810 sans l autorisation du fabricant Cela pourrait entra ner un incendie ou une d charge lectrique e Ne placez ou ne faites tomber aucun objet sur le QuickGene 810 De m me vitez de lui faire subir des chocs ou des coups cela pourrait entra ner des d faillances ou un mauvais fonctionnement de l appareil e Si un quelconque liquide s introduit dans l appareil pongez le avec une serviette en papier sinon le QuickGene 810 pourrait tre endommag e Le capot avant ne tient pas tant qu il n a pas atteint sa limite sup rieure d ouverture Si vous l chez le capot avant en l ouvrant il risque de blesser quelqu un ou d endommager l appareil Lorsque vous remplacez le tuyau de pompe veillez porter des gants e Au cas o de l thanol se renversait d branchez imm diatement le c ble d alimentation Des r parations du QuickGene 810 ne doivent tre
23. how to install the Tube Cap 1 Set the Tube Cap on the conical tube e Set the purple cap on the conical tube for wash buffer right Set the blue cap on the conical tube for elution buffer left Le 2 Connect the Tube Cap and the Pump Tube Check the combination of the Pump Tube and Tube Cap label and then turn the connector to connect them as shown in the figure Connect the Pump Tube for the Wash Buffer Pump upper purple and the purple Tube Cap Connect the Pump Tube for the Elution Buffer Pump lower blue and the blue Tube Cap Tube Cap Purple To Wash Buffer Pump Tube Cap Blue To Elution Buffer Pump Mm Setup the Holder Carriage The following explains how to install the Holder Carriage 1 Attach the Holder Stand to the QuickGene 810 Insert the tabs of the Holder Stand in the slits on the QuickGene 810 Note After attaching the Holder Stand check that there is no gap between the bottom of Holder Stand and the QuickGene 810 2 Set Tube Holder and Cartridge Holder in the Holder Carriage Align the tab and notch QuickGene 810 User s Guide Rev 1 3 13 1 Installation 3 Attach the Holder Carriage which was prepared in 2 to the QuickGene 810 Hold the handles of the Holder Carriage and attach the Holder Carriage onto the Holder Stand of the QuickGene 810 After setting it check the following Check that the Cartri
24. opening it it may cause injury or device damage 5 Attach the Pump Tube to the pump Attach the Pump Tube to both the Wash Buffer Pump and the Elution Buffer Pump Important When attaching the Pump Tube wear gloves Do not touch the Drive Head in the pump without gloves Drive Head QuickGene 810 User s Guide rev 1 3 1 Installation a Detach the pump cover Le Loosen and remove the screws to detach the pump cover Z SS lt CA b Attach the Pump Tube to the rear of the Pump Cover Slide the connectors at the ends of the Pump Tube into the grooves of the Pump Cover Check that the notches on both connectors are facing the front of the Pump Cover Slide the Pump Tube into place inside the Pump Cover by rotating the rotor Adjust the rotor to its original vertical position p Pump Tube Use the Pump Tube as labeled ELUTION Connector Pump Tube or WASH Pump Tube for each pump Groove Spare pump tube is available for either pump Slide the Pump Tube Notches face front connector into groove Important Check the both end of the Pump Tube are placed the grooves c Attach the pump cover Pumpicover Rear Turn the rotor vertical position then re attach Drive Head Drive Socket EY the pump cover to the retainer making sure that the drive head and drive socket are Nap a correctly mated
25. perform the following tasks Remove all attached parts from the QuickGene 810 Secure the tubes inside the QuickGene 810 with tape 1 3 Remove the Packaging Materials page 4 Secure the carrier with the 2 clamps so that it does not move Secure the front cover with tape so that it will not open 1 3 Remove the Packaging Materials page 4 After completing the above tasks pack the QuickGene 810 in its original shipping box or some other equivalent box QuickGene 810 User s Guide Rev 1 3 Nucleic Acid Isolation System QuickGene 810 User s Guide P3X1 E370 04EN 00 Date of issuance December 2005 Fuji Photo Film Co Ltd Printed in Japan The contents of this manual may be updated without notice Fuji Photo Film Co Ltd assumes no liability for damages to third party copyrights or other rights arising from the use of any information in this manual Contents of this manual are not to be reproduced without permission from Fuji Photo Film Co Ltd Manuals with missing or wrongly collated pages will be replaced free of charge Bj FUJIFILM FUJI PHOTO FILM CO LTD 7 3 E lt g S nuh 2 al Im al Wit P3X1 E370 04EN
26. to the paused position Operations restart beginning with the cartridge that was in operation e If the operation is paused while applying pressure pressure application is performed again starting with the applicable cartridge During washing During elution e All operations stop except for wash buffer pump and elution buffer pump e During the wash buffer or elution buffer discharge process the operations stop after discharge for one cartridge If stopped while applying pressure The pressure head moves to the paused position and only pressure application restarts e If wash buffer or elution buffer was being discharged operations restart with the next cartridge If stopped when pressure application was completed or with no pressure application e The pressure head moves to the next cartridge of the cartridge being processed then pressure application and wash buffer discharge processes restart 28 QuickGene 810 User s Guide rev 1 3 3 Operation 3 6 Operations to Stop Extraction Process To stop the extraction operation perform the following procedures 1 Press the STOP button or open the front cover The LCD displays a message as follows Ifthe STOP button is pressed START SW gt RESTART If the front cover is opened COVER OPEN 2 In the case when the front cover is opened close the front cover 3 When the LCD displays START SW RESTART press the RESET bu
27. 0 disconnect the plug from the outlet first If the power cable is damaged this may cause a device failure fire injury or electric shock A CAUTION e Do not install the QuickGene 810 in an unstable place such as a i slanted surface or a place subject to vibrations It may cause injury or device failure Do not install the QuickGene 810 in direct sunlight or close to a heating device It may shorten the life of QuickGene 810 or cause a trouble Two or more people are necessary to carry the QuickGene 810 Otherwise you may hurt your back or hands because of the weight of QuickGene 810 more than 20kg For correct and safe use of QuickGene 810 install it in a location that meets the following conditions The space shown in the following figure must be allocated Top 30cm Left 5cm Right and Rear 15cm 7 A location where power can be provided Use an AC100 10 to AC240V 10 50Hz or 60Hz outlet A location where the temperature is 15 to 30 C and humidity is 30 to 80 RH non condensing A location that will support the weight of QuickGene 810 21kg that is flat and stable with no vibration QuickGene 810 User s Guide Rev 1 3 1 1 Installation A location away from direct sunlight Block the sunlight by curtains or blinds as necessary e A location which is well ventilated and not dusty A location where the temperature does not go up and down suddenly W
28. 00 Huai Hai Zhong Road Shanghai P R China Tel 86 21 3302 4655ext 363 Fax 86 21 6384 3322 E mail wgxiang fujifilm com cn URL http www fujifilm com cn QuickGene 810 User s Guide Rev 1 3 49 Distributors lt Australia New Zealand gt Berthold AUSTRALIA PTY Ltd 40 Clements Avenue BUNDOORA Victoria 3083 Australia Tel 61 3 9467 6277 1 300 300 865 in Australia Fax 61 3 9467 7493 E mail rafael berthold com au URL http berthold com au lt Korea gt Shinki Hi Tec GUNWHA Bldg 7 1 Yangjae 1 dong Secho gu Seoul 137 886 Korea Tel 82 2 572 1600 Fax 82 2 572 0058 E mail info skhitec co kr URL http www skhitec co kr lt Taiwan gt HUNG CHONG CORP No 38 Sec 6 Min Chuan E Road Taipei Taiwan Tel 886 2 2791 1188 Fax 886 2 2794 2248 E mail fuhsing mail hungchong com tw URL http www FUJIFILM COM TW A 6 Notes for Transfer QuickGene 810 50 When transferring the QuickGene 810 note the following points When carrying the QuickGene 810 disconnect the plug from the outlet AN WARNING first Watch your step when you relocate the QuickGene 810 If the power cable is damaged this may cause a device failure fire injury or electric shock Two or more people are necessary to carry the QuickGene 810 A CAUTION Otherwise you may hurt your back or hands because of the weight of QuickGene 810 more than 20kg When transferring the QuickGene 810 be sure to
29. 14 ISOLATE G 32 DNA YEAST 15 ISOLATE H 33 DNA CULTURE CELL 16 RNA BLOOD 34 DNA HAIR NAIL 17 RNA BLOOD PLUS 35 DNA BUFFYCOAT 18 PLASMID B m How to change extraction mode names The following explains how to change extraction mode names 1 Select the extraction mode Press the MODE button several times until the target extraction mode appears on the LCD 2 Press the A W buttons simultaneously Setup mode starts 3 Press the MODE or DISCHARGE button several times until the following message is displayed on the LCD RENAME MODE Y gt A 4 Press the 4 button RENAME mode starts 5 Press the A or W button several times until the extraction mode name to be applied is displayed on the LCD QuickGene 810 User s Guide Rev 1 3 43 5 How to Set Parameters 6 Press the START button when the mode name to be applied is displayed on the LCD Display on operation panel After the name before being changed is displayed on the LCD a message to ask whether to save the DNA WHOLE BLOOD new name is displayed Extraction mode name before change RENAME Y N gt V Ask whether to save the new name or not 7 Press the A button and save the new name Display on operation panel When the LCD displays as shown in the figure on the left the changed name is saved and the mode NAME WRITING returns to the USER mode Save the new name Important When not saving the new na
30. 2 750 Can be specified between 250 750hl in increments of 501 Sets the third wash buffer discharge rate 6 WASH VOL3 250 3rd WBDR 9 a WASH VOL3 750 Can be specified between 250 750 in increments of 501 7 WASH VOL4 250 4th WBDR Sets the fourth wash buffer discharge rate WASH VOL4 750 Can be specified between 250 750hl in increments of 501 8 WASH VOL5 250 th WEDR Sets the fifth wash buffer discharge rate WASH VOLS 750 Can be specified between 250 750hl in increments of 501 Sets the wash buffer immersion management time 5 WASH DIP TM 0 no ae WASH DIP TM 510 Can be specified between 0 510 sec in increments of 2 management timer seconds Sets the time to wait for shifting to wash buffer discharges in WAS2 WAIT T 0 SEE the second round 10 Waiting time a oo WAS2 WAIT T 255 Can be specified between 0 255 min in increments of 1 minute Sets the frequency of wash buffer discharges in the second m aszcounr oo ona enoney WAS2 COUNT 5 g the 2nd round Can be specified between 0 5 times Sets the peak pressure during pressure application Judgment criteria is the pressure before pressure application the WAS2 PEAK 50 Wash buffer peak pressure set in this item Pressure application may be stopped 12 WAS2 PEAK 160 pressure in the 2nd before reaching the peak pressure if air pressure drops round suddenly indicating that all liquid has been discharged Can be specified between 50 160 kpa in increments of
31. 60 kpa 160 160 160 160 160 160 160 6 BIND LOWER 3 100 kpa 75 75 75 75 75 75 75 7 BIND DOWN TM 1 99 sec 25 25 25 25 25 25 25 8 BINDRDNT 1 100 sec 50 50 50 50 50 50 50 9 BIND FALL 5 60 kpa 60 60 60 60 60 60 60 10 WASH COUNT 0 5 time s 3 3 1 3 3 1 11 WASH SPEED 1 12 7 7 7 7 7 7 12 WASH PEAK 50 160 kpa 110 110 110 110 110 110 110 13 WASH UP TIME 1 50 sec 6 6 6 6 6 6 6 14 WASH RETRY 50 160 kpa 140 140 140 140 140 140 140 15 WASH LOWER 3 100 kpa 70 70 70 70 70 70 70 16 Le DOWN 1 99 sec 15 15 15 15 15 15 15 17 WASH R DN T 1 100 sec 50 50 50 50 50 50 50 18 WASH FALL 5 60 kpa 60 60 60 60 60 60 60 19 WASH VOL1 250 750 HI 750 500 500 750 750 750 750 20 WASH VOL2 250 750 HI 750 500 500 750 750 750 750 21 WASH VOL3 250 750 HI 750 500 500 750 750 750 750 22 WASH VOL4 250 750 HI 750 500 500 750 750 750 750 23 WASH VOL5 250 750 HI 750 500 500 750 750 750 750 24 WASH DIP TM 0 510 sec 0 150 150 0 150 150 25 WAS2 WAIT T 0 255 min 0 0 5 0 0 5 26 WAS2 COUNT 0 5 time s 0 0 2 0 0 2 0 27 WAS2 SPEED 1 12 7 7 7 7 7 7 7 28 WAS2 PEAK 50 160 kpa 110 110 110 110 110 110 110 29 WAS2 UP TIME 1 50 sec 6 6 6 6 6 6 6 30 WAS2 RETRY 50 160 kpa 140 140 140 140 140 140 140 31 WAS2 LOWER 3 100 kpa 70 70 70 70 70 70 70 32 es DOWN 1 99 sec 15 15 15 15 15 15 15 33 WAS2 R DN T 1 100 sec 50 50 50 50 50 50 50 34 WAS2 FALL 5 60 kpa 60 60 60 60 60 60 60 35 WAS2 VOL1 250 750 HI 750 500 500 750 750 750 750 36 WAS2 VOL2 250
32. 908090809080809 0809090908 0 090909090909090909090909090909090909090 0909090909090909090902090909090909090 090909090909090909090909090909090909090 0900090909090009000909090900090009000 0909092090 2090 9090900090 02090 909090009000 0909090909090909090909090909090909090 090909090909090909090909090909090909090 0309090909090903090909090909090909090 090909090909090909090909090909090909090 0909090909090909090902090909090909090 030909090909090909090909090909090909090 0900090909090009000909090900090009000 0909092090 2090 9090902090 02090 909090009000 0909090909090909090909090909090909090 090909090909090909090909090909090909090 0909090909090909030909030909090909090 0909090909090 709020900090 909090009000 0900090000000 9000909020000090009000 0909090909090 309090909090909090909090 0309090909090 33090909090909090909090 0909090909090 309090909090909090909090 0909090902090 39090902090909090902090 0909090909090 309090909090909090909090 0909090909090 33090909090909090909090 0309090909090 709020900090 909090009000 0909090909090 309090909090909090909090 909000000000000 00000000000000000000000 Ventilation Holes Breaker Checkpoint Inlet QuickGene 810 User s Guide Rev 1 3 1 Installation Internal view me 7 f RN i N Carrier i Il Wash Buffer C 2222 Pump in
33. Front cover Earth leakage breaker LCD Lamp LED and buzzer m Front cover When the front cover is open operations such as extraction processes cannot be performed Also if the front cover is opened during the extraction process it will be temporarily interrupted However when the buffer pump is working the pump will continue to work until the step is completed Operation can be restarted from this point in the process by pressing the start button Note During the extraction process do not open the front cover unless it is necessary m Earth leakage breaker When there is an electric leak or current surge caused by failures of the QuickGene 8 10 the earth leakage breaker will cut off the power supply If the earth leakage breaker trips contact your local representative m LCD Lamp LED and buzzer The LCD Lamp LED and buzzer display the operating status of the QuickGene 8 10 16 QuickGene 810 User s Guide rev 1 3 2 Basic Functions 2 4 LCD Display Information The following describes the messages displayed in the LCD during normal operations For the messages displayed in case of errors refer to 6 3 Error Messages page 46 Message Contents FIRMWARE FVxxLxx Shows the Software version This appears when you turn on the power WAIT AMOMENT Initializing the QuickGene 810 or disposing of the waste DNA WHOLE BLOOD mode is set to operate on the QuickGene 810 DNA WHOLE BLOOD The operatio
34. Life Science w FUJIFILM User s Guide Nucleic Acid Isolation System QuickGene 810 Rev 1 3 P3X1 E370 04EN Introduction Thank you for using the Nucleic Acid Isolation System QuickGene 810 hereafter called QuickGene 810 Easy operation of this device enables extraction of DNA and RNA This guid contains important information regarding the safe use of the QuickGene 810 Read this manual carefully before using Keep this manual after reading for reference whenever necessary About Infectious Materials When handling or disposing of infectious materials follow the laboratory guideline or the law regarding infectious wastes to perform the incineration fusion sterilization and or disinfection When you use a third party to dispose it outsource it to a licensed operator for industrial waste subject to special control and give them the management table Universal biological manifest for industrial waste subject to special control at the same time hazard symbol m Exclusion clauses e The contents of this manual may be updated without notice e Fuji Photo Film Co Ltd assumes no liability for damages to third party copyrights or other rights arising from the use of any information in this manual Fuji Photo Film Co Ltd assumes no liability for device failures or damages due to installation relocation remodeling maintenance and repairs perfo
35. Occasionally a temporary conductivity that is caused by condensation must be expected This location is a typical office home environment Temporary condensation occurs only when the product is out of service Pollution Degree Pollution Degree 2 Note Rated for indoor use only QuickGene 810 User s Guide rev 1 3 1 Installation 1 2 Checking the QuickGene 810 and Accessories Check that the following are included in the package Contact your local representative if you notice any missing part s QuickGene 810 QuickGene 810 User s Guide O Holder Carriage O Cartridge Holder O Tube Holder O Discharge Tray O Tube Rack O Pump Tube O Pump Tube O Pump Tube for wash buffer for elution buffer spare tube O Tube Cap Large Blue O Tube Cap Small Blue O Tube Cap Large Purple O Collection Tube CT O Waste Tube WT O Cartridge CA i y For the options refer to A 2 Options gt page 48 QuickGene 810 User s Guide Rev 1 3 3 1 Installation 1 3 Remove the Packaging Materials After unpacking the QuickGene 810 from the box do as follows a 1 Remove the tape that holds the front cover Tape 2 Open the front cover and remove the following items Clamps for fixing the carrier both side of the Carrier Remove the tape that holds the tubes Important Keep the shipping box and 2 clamps They will be necessary for tran
36. Sensor F Elution Buffer 11 I Pump A Al Pressure Head Wash Buffer Nozzle h A Elution Buffer nz Nozzle O c LT Holder Stand Buffer stand Name Function Set the conical tubes to contain elution buffer and wash buffer and the Buffer Stand Discharge Tray Set the Tube Holder and Cartridge Holder Holder Stand Set collection tubes and waste tubes in the Tube Holder Set cartridges in the Cartridge Holder Wash Buffer Pump Sends the wash buffer in the conical tube to the Carrier Elution Buffer Pump Sends the elution buffer in the conical tube to the Carrier Carrier Moves automatically during extraction operation Pressure Head Presses out the air and uses the pressure to flush out solutions Wash Buffer Nozzle Provides the wash buffer into Cartridges Elution Buffer Nozzle Provides the elution buffer into Cartridges Sensor Checks that the carrier is placed in the correct position Do not touch the sensors QuickGene 810 User s Guide rev 1 3 m Operation Panel 1 LCD STOP RESET 1 Installation BINDING WASHING ELUTION POWER Se gt amp START CRMOVE MODE READY C3
37. TION Important Important Note Note QuickGene 810 User s Guide Rev 1 3 QuickGene 810 User s Guide Rev 1 3 Introduction Merci d avoir choisi le Syst me d isolation de l acide nucl ique QuickGene 810 appel ci apr s QuickGene 810 Le fonctionnement simple de cet appareil permet l extraction d ADN et d ARN Ce manuel contient des informations importantes concernant l utilisation en toute s curit du QuickGene 810 Lisez attentivement ce manuel avant toute utilisation Apr s lecture conservez ce manuel pour pouvoir vous y r f rer ult rieurement si n cessaire propos des produits infectieux Lorsque vous manipulez ou mettez au rebut des produits infectieux veillez respecter les directives du laboratoire ou la loi les concernant pour en effectuer l incin ration la fusion la st rilisation et ou la d sinfection Lorsque qu un tiers est charg de le faire veillez le confier une entreprise habilit e traiter des d chets industriels sujets contr le sp cifique et fournissez Universal biological leur en m me temps le tableau de gestion le manifeste des d chets industriels hazard symbol sujets contr le sp cifique Clauses d exclusion e Le contenu de ce m
38. anged from the normal setup mode USER mode and others are changed from the EXPERT mode The following table shows the display sequence setting range default value etc for each parameter in USER mode Mode name The value displays the default ence eau ee ee D BLOOD PLUS PLUS 1 BIND PEAK 50 160 kpa 120 120 120 120 120 120 120 2 WASH COUNT 0 5 time s 3 3 1 3 3 1 2 3 WASH PEAK 50 160 kpa 110 110 110 110 110 110 110 4 WASH VOL1 250 750 HI 750 500 500 750 750 750 750 5 WASH VOL2 250 750 HI 750 500 500 750 750 750 750 6 WASH VOL3 250 750 HI 750 500 500 750 750 750 750 7 WASH VOL4 250 750 HI 750 500 500 750 750 750 750 8 WASH VOL5 250 750 HI 750 500 500 750 750 750 750 9 WASH DIP TM 0 510 sec 0 150 150 0 150 150 0 10 WAS2 WAIT T 0 255 min 0 0 5 0 0 5 0 11 WAS2 COUNT 0 5 time s 0 0 2 0 0 2 0 12 WAS2 PEAK 50 160 kpa 110 110 110 110 110 110 110 13 WAS2 VOL1 250 750 HI 750 500 500 750 750 750 750 14 WAS2 VOL2 250 750 HI 750 500 500 750 750 750 750 15 WAS2 VOL3 250 750 HI 750 500 500 750 750 750 750 16 WAS2 VOL4 250 750 HI 750 500 500 750 750 750 750 17 WAS2 VOL5 250 750 HI 750 500 500 750 750 750 750 18 ELUT VOL 50 400 HI 200 100 100 200 100 100 50 19 ELUT PEAK 50 160 kpa 100 100 100 100 100 100 100 20 ELUT DIP TM 0 510 time s 0 30 30 90 30 30 0 QuickGene 810 User s Guide Rev 1 3 37
39. anuel peut tre mis jour sans pr avis Fuji Photo Film Co Ltd ne peut tre tenu pour responsable de dommages aux copyrights de tiers ou d autres droits manant de l utilisation d une information quelconque contenue dans ce manuel Fuji Photo Film Co Ltd ne peut tre tenu pour responsable de d faillances ou de dommages de l appareil caus s par l installation la relocalisation la modification la maintenance et les r parations effectu s par des tiers autres que Fuji Photo Film Co Ltd ou des agences agr es par Fuji Photo Film Co Ltd e Fuji Photo Film Co Ltd ne peut tre tenu pour responsable des d faillances ou des dommages de ses produits dus des produits tiers autres que ceux fournis par by Fuji Photo Film Co Ltd e Fuji Photo Film Co Ltd ne peut tre tenu pour responsable des d faillances ou des dommages de l appareil dus la modification la maintenance les r parations etc utilisant des pi ces de rechanges qui ne sont pas des pi ces d origine sp cifi es par Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd ne peut tre tenu pour responsable des d faillances ou des dommages de l appareil dus la non observation des proc dures de fonctionnement et des pr cautions d crites dans ce manuel e Fuji Photo Film Co Ltd ne peut tre tenu pour responsable des d faillances ou des dommages de l appareil en cas o il est utilis dans un environnement qui ne r pond pas aux co
40. arming a cold room suddenly or moving the QuickGene 810 from a room with low temperature to a warm room may cause condensation inside the device condensing it may damage the QuickGene 810 system A location far from water taps water heaters humidifiers air conditioners and heaters Avoid areas of high temperature and high humidity or low temperature and low humidity A location far from objects which generate strong magnetic fields motors transformers TV audio speakers magnets etc Bringing the QuickGene 810 close to any type of magnetic field may cause a malfunction m Use conditions The following shows the operating environment of the QuickGene 810 Items Specifications During operation 15 30 Temperature C During downtime 0 55 o During operation 30 80 Humidity RH During downtime 10 80 Max wet bulb During operation 29 non condensing temperature C During downtime 29 non condensing Temperature gradient C Hr 12 or less non condensing Humidity gradient RH day 30 or less non condensing Altitude operating m 2 000 Installation Overvoltage Category Definition CAT II Local level mains wall sockets Equipment at this level includes appliances portable tools and similar products Equipment is usually cord connected Pollution Degree Definition Pollution Degree2 Normally only dry nonconductive pollution occurs
41. aterials 4 1 4 NERVEN 0 en a ee ee 5 1 5 MUD PAS sentent etienne toe E trente 9 1 6 Checking QuickGene 810 Operation 14 2 Basic Functions 2 1 Componente ee onu or on 15 2 2 Working Process of QuickGene 810 pp 16 2 3 Safety Mechanism ne 16 2 4 LCD Display Information een 17 2 5 Main Speciisalans na een 18 3 Operation 3 1 Turn the Power ON and OFF pp 19 3 2 Preparations nee e tee 20 3 3 Preparation of Lysate Samples 21 3 4 Extraction Operations ann una 21 3 5 Operations to Restart Program from Pause ppp 28 3 6 Operations to Stop Extraction Process 29 3 7 Quick OUId6 0 ee el 29 4 Maintenance 4 1 Before Using the QuickGene 810 31 4 2 When the QuickGene 810 is left for a Week or More 31 4 3 Cleaning the QuickGene 810 pp 32 4 4 Cleaning the PAINS a a wed 33 4 5 How to Replace the Pump Tube 33 5 How to Set Parameters 5 1 Parameters 0 a e aae naia ie 37 5 2 How to Set Change Parameters 39 5 3 Parameters in EXPERT Mode en 41 5 4 Changing Extraction Mode Names 43 QuickGene 810 User s Guide Rev 1 3 xix XX 6 Troubleshooting 6 1 Extraction Falle a AE 45 6 2 Typical Failure sen Gi eana oraaa aAa 45 6 3 Error Messages 0 oa ceciaiee deeecte 46 6 4 When the Buffer is Contaminated pp 46 Appendix A A 1 Usable Power Supply CordS nn nneeenne 47 A 2 OplloNS 48 A 3 PROOUL SENIORS a ee rics Seen 49 A 4
42. ausschlie lich mit der am Ger t angegebenen Netzspannung berlasten Sie die Steckdose nicht durch Anschlie en von zu vielen Ger ten Verwenden Sie das QuickGene 810 nicht mit einem besch digtem Netzstecker oder einer lockeren Steckdose Wenn die Kontakte des Netzsteckers oder die Steckdose staubig sind wischen Sie sie mit einem trockenen Tuch ab Wenn Sie den Netzstecker aus der Steckdose ziehen fassen Sie stets den Netzstecker direkt an Ziehen Sie nicht am Netzkabel Ziehen Sie vor der Durchf hrung von Wartungsarbeiten den Netzstecker ab Fassen Sie den Netzstecker bei Gewitter nicht an e Versch tten Sie keine Fl ssigkeiten ber dem QuickGene 810 Stellen Sie keine Fl ssigkeitsbeh lter auf dem QuickGene 810 ab Dadurch k nnte es zu einem Ausfall des Ger tes zu einem Brand oder Stromschlag kommen e Wenn sich das Ger t berhitzt oder wenn Rauch oder ungew hnliche Ger che auftreten ziehen Sie sofort das Netzkabel ab QuickGene 810 User s Guide Rev 1 3 XV XVi A CAUTION Versuchen Sie nicht das QuickGene 810 ohne die Erlaubnis des Herstellers zu ver ndern Dadurch k nnte es zu einem Brand oder Stromschlag kommen Legen Sie keine Gegenst nde auf dem QuickGene 810 ab und lassen Sie keine Gegenst nde darauf fallen Setzen Sie es au erdem keinen St en oder hnlichen Ersch tterungen aus da es dadurch zu einem Ausfall oder zu einer Fehlfunktion des QuickGene 810 kommen k nnte Wenn im Ger t irgendwelc
43. de the upper part of Cartridge Holder as shown in the figure Tilt the cartridge holder a little to make it easier to drop down the cartridges Dispose of the cartridges into a waste container 7 Check the Holder Stand and Discharge Tray Discharge Tray If waste remains on them dispose of it Note When the carrier is on the left and it is difficult to remove the Discharge 9 Tray close the front cover and press ol 6 the CR MOVE button 8 Set the Cartridge Holder and Tube Holder Stand Holder in the Holder Carriage and then restore them to the QuickGene 810 WSetup the Holder Carriage page 13 9 Close the front cover The operation has been completed Turn off the power of QuickGene 810 Dispose of the remaining buffer Clean the dirty parts Refer to 4 Maintenance page 31 for maintenance details Important After QuickGene 810 operation if it will not be used for one week or more put 7ml of ethanol in both conical tubes then execute the discharge operation 4 times After the discharge operation check the tube is no ethanol remaining gt 4 Maintenance page 31 QuickGene 810 User s Guide Rev 1 3 27 3 Operation 3 5 Operations to Restart Program from Pause When the front cover is opened during PROCESSING Then the program is halted and the LCD displays COVER OPEN If this occurs while the wash buffer pump or elution buffer pum
44. dge Holder is firmly in place Check that the Tube Holder rests firmly on the metal shelf and two pins are placed in the holes Set in the notch Check that the two pins are aligned the holes Note When the carrier is in the center and it is difficult to attach the Holder Carriage close the front cover and press the CR MOVE button Then the carrier moves to the end 4 Close the front cover 1 6 Checking QuickGene 810 Operation This section explains how to check the operation of QuickGene 810 After all the parts are installed check the operation of QuickGene 8 10 To check the operation use sterile distilled water instead of buffers and dissolved samples and perform the same operation as the normal extraction operation For the extraction operation refer to 3 Operation page 19 14 QuickGene 810 User s Guide rev 1 3 2 Basic Functions 2 Basic Functions This chapter explains the basic functions of the QuickGene 810 2 1 Components The following describes the main components of the QuickGene 8 10 gt TN Operation unit a SP eae 7 gt Z ump unit Il KS a Carrier unit gt Kl au Sample unit Buffer setting unit Component Function Operate the buttons to select an extraction mode and start stop an extraction Operation unit process The LCD and LEDs display the device status The
45. e event the device overheats starts to smoke or smells strange immediately unplug the power cable N CAUTION e Never attempt to remodel the QuickGene 810 without the manufacturer s permission Doing so may cause fire or electric shock Do not place or drop objects on the QuickGene 810 Also refrain from bumping or knocking it as doing so may cause a failure or malfunction of the QuickGene 810 e If any liquid materials are left inside the device wipe with a Kimwipe or paper tissue Otherwise the QuickGene 810 may be damaged The front cover will not stay on the way to the upper limit If you release the front cover while opening it it may cause injury or device damage e When replacing the Pump Tube be sure to wear gloves Inthe event of an ethanol spill immediately unplug the power cable e Repairs to the QuickGene 810 should only be performed by such agencies as are specifically authorized by Fuji Photo Films Co Ltd e Only original Fuji Photo Films Co Ltd replacement parts should be used e If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired QuickGene 810 User s Guide Rev 1 3 ix About Installation Conditions Do not install the QuickGene 810 to a location where it can be AN WARNING splashed with water Doing so may cause a device failure fire or electric shock When relocating the QuickGene 810 be sur
46. e to disconnect the plug from the outlet first Watch your step when you relocate the QuickGene 810 If the power cable is damaged this may cause a device failure fire injury or electric shock Do not install the QuickGene 810 in an unstable place such as a A CAUTION Be 5 slanted surface or a place subject to vibrations Doing so may cause injury or device failure e Do not install the QuickGene 810 in direct sunlight or close to a heating device Doing so may shorten the life of QuickGene 810 or cause a failure Two or more people are necessary to relocate the QuickGene 810 Otherwise you may hurt your back or hands because of the weight of QuickGene 810 more than 20kg For correct and safe use of QuickGene 810 install it in a location that meets the following conditions A location where power can be provided Use an AC100 10 to AC240V 10 50Hz or 60Hz outlet A location where the temperature is 15 to 30 C and humidity is 30 to 80 RH non condensing A location that will support the weight of QuickGene 810 21kg that is flat and stable with no vibration A location away from direct sunlight Block the sunlight by curtains or blinds as necessary A location which is well ventilated and not dusty A location where the temperature does not go up and down suddenly Warming a cold room suddenly or moving the QuickGene 810 from a room with low temperature to a warm room may cause condensation inside the device c
47. effectu es que par des agences sp cifiquement autoris es par Fuji Photo Films Co Ltd Et seules doivent tre utilis es les pi ces d tach es originales provenant de Fuji Photo Films Co Ltd e Si ce mat riel est utilis de mani re non sp cifi e par le fabricant la protection fournie par le mat riel peut s en trouver diminu e QuickGene 810 User s Guide Rev 13 xiii A propos des conditions d installation ze xiv N installez le QuickGene 810 dans un emplacement o il pourrait tre AN WARNING eclabousse par de l eau Cela pourrait entra ner une d faillance de l appareil un incendie ou une d charge lectrique e Si vous devez d placer le QuickGene 810 vers un autre emplacement veillez d brancher l appareil en premier Faites attention en d pla ant le QuickGene 810 si vous marchez sur le c ble d alimentation et qu il est endommag cela peut entra ner une d faillance de l appareil un incendie ou une d charge lectrique A CAUTION e N installez pas le QuickGene 810 sur un emplacement instable comme par exemple une surface inclin e ou sujette vibrations Cela pourrait entra ner des blessures ou un dysfonctionnement e Ne placez pas le QuickGene 810 sous l exposition directe du soleil ou proximit d un appareil de chauffage Cela pourrait raccourcir la dur e de vie du QuickGene 810 ou provoquer un dysfonctionnement e Pour d placer le QuickGene 810 deu
48. em Brand oder Stromschlag kommen A CAUTION e Stellen Sie das QuickGene 810 nicht auf einem unsicheren Ort auf z B auf einer absch ssigen Oberfl che oder an einem Ort der Vibrationen ausgesetzt ist Dadurch kann es zu Verletzungen oder einem Ausfall des Ger ts kommen e Stellen Sie das QuickGene 810 nicht an einem Ort auf der direktem Sonnenlicht ausgesetzt ist oder in der N he eines Heizger tes liegt Dadurch k nnte die Lebensdauer des QuickGene 810 verk rzt oder ein Ausfall verursacht werden e Eine Ver nderung des Aufstellungsorts des QuickGene 810 muss von mindestens zwei Personen durchgef hrt werden Ansonsten kann es aufgrund des Ger tegewichts ber 20 kg zu Verletzungen des R ckens oder der H nde kommen F r eine problemlose und sichere Verwendung stellen Sie das QuickGene 810 an einem Ort auf der die folgenden Bedingungen erf llt Stromversorgung Verwenden Sie eine Steckdose mit einer Netzspannung von 100 10 bis 240 V 10 AC 50 Hz oder 60 Hz Temperatur 15 bis 30 C und relative Luftfeuchtigkeit zwischen 30 und 80 nicht kondensierend Eine ebene stabile Oberfl che die das Gewicht des QuickGene 810 21 kg tr gt und die keinen Vibrationen ausgesetzt ist Kein direktes Sonnenlicht Falls erforderlich sch tzen Sie das Ger t durch Vorh nge oder Jalousien vor direkter Sonneneinstrahlung Gut bel ftete und staubfreie Umgebung Keine abrupten Temperaturschwankungen Wenn ein kalter Raum
49. em blade UL CSA Approved 3 Conductors Single Rated 10 A 250 Vac phase 2 current carrying UL CSA Approved conductors amp ground SP UL CSA Approved a Europe 100 240 IEC60320 C 22 CENELEC 0C 3X1 0 Rated 10 A 250 Vac lt 2 gt Vac Rated 10A 250Vac square mm lt 1 gt lt 1 gt lt 1 gt lt HAR gt Australia 100 240 IEC60320 C 22 Cable AS OD 3 X1 0 Rated 10 A 250 Vac Vac Rated 10A 250Vac square mm e g Dr Sr x Le L U K 100 240 IEC60320 C 22 BS OC 3 X1 00 square mm Rated 10 A 250 Vac lt 2 gt Vac Rated 10A 250Vac Others Standards compliant with ground conductor and rated 10A or better lt 1 gt Note 1 Be sure that the detachable proper Supply cord has the approval of the appropriate safety agencies of the country where the equipment will be used Note 2 Cable length of above Power Supply cord shall be shorter than 4 5 m QuickGene 810 User s Guide Rev 1 3 47 A 2 Options l Name Holder Carriage Name Cartridge Holder Name Tube Holder Name Adapter Name Discharge Tray S Name Pump Tube Name Tube Cap Large Blue Name Tube Cap Large Purple Name Tube Cap Small Blue Name Tube Rack 4pcs Name QG810 Carriage Set 48 QuickGene 810 User s Guide Rev 1 3 A 3 About Services Before you ask for device repairs refer to 6 Troubleshooting page 45 to check the problems If the problems cannot
50. ene 810 User s Guide Rev 1 3 1 Installation 1 5 Setup Parts This section explains how to install the parts included in the package Install the following parts Power Cable Pump Tube Buffer Stand Tube Cap Holder Stand An empty conical tube is needed to set a Tube Cap Prepare two conical tubes in order to set Tube Caps for elution buffer and wash buffer respectively The recommended conical tubes are as follows Size Type Item Sandara Large conical tube for wash buffer BD Falcon 50ml conical tube Small conical tube for elution buffer BD Falcon 15ml conical tube Large conical tube for wash buffer BD Falcon 175ml conical tube Large Small conical tube for elution buffer BD Falcon 50ml conical tube Mm Setup the power cable The following explains how to install the power cable AN WARNING Ignoring the following notations may lead to fire or electric shock Use a power cable that meets your country s standard or contact your local distributor Si les recommandations suivantes ne sont pas prises en compte un incendie ou une d charge lectrique peuvent se produire Utilisez un c ble d alimentation conforme aux r glementations de votre pays ou contactez votre revendeur local Bei Missachtung der folgenden Warnhinweise besteht Brand und Strom
51. hanging other items repeat steps 3 and 4 39 5 How to Set Parameters 5 Press the START button then save the changed parameters The saved parameters are written into non volatile memory The data will not be lost even if the power is turned off Display on operation panel When the LCD displays as shown in the figure at the left the changed settings are saved and the setup SETUP WRITING mode ends Save the settings Important To cancel the saving parameters press the STOP or RESET button SETUP COMPLETED The setup mode ends without saving the changed parameters DNA WHOLE BLOOD Exit setup mode and display current extraction mode j 40 QuickGene 810 User s Guide Rev 1 3 5 How to Set Parameters 5 3 Parameters in EXPERT Mode In EXPERT mode in addition to the USER mode parameters values of special parameters can also be changed Important You must not change any parameters without our guidance The following table shows the display sequence setting range default value etc for each parameter in EXPERT mode Mode name The value displays the default ee BLOOD PLUS PLUS 1 BIND COUNT 1 5 1 1 1 1 1 1 1 2 BIND SPEED 1 12 10 10 10 10 10 10 10 3 BIND PEAK 50 160 kpa 120 120 120 120 120 120 120 4 BIND UP TIME 1 50 sec 6 6 6 6 6 6 6 5 BIND RETRY 50 1
52. he Fl ssigkeiten zur ckbleiben wischen Sie es mit einem fusselfreien Papiertuch aus Andernfalls kann es zu einer Besch digung des QuickGene 810 kommen Die vordere Abdeckung rastet nicht vor Erreichen des oberen Anschlags ein Wenn Sie die vordere Abdeckung loslassen w hrend Sie sie ffnen kann es zu Verletzungen oder zu einer Besch digung des Ger ts kommen Tragen Sie beim Auswechseln des Pumpenrohrs stets Handschuhe Falls Ethanol versch ttet werden sollte ziehen Sie sofort das Netzkabel ab Reparaturarbeiten am QuickGene 810 sollten nur von Firmen ausgef hrt werden die von Fuji Photo Films Co Ltd ausdr cklich daf r autorisiert sind Dabei sollten nur Original Ersatzteil von Fuji Photo Films Co Ltd verwendet werden Wenn das Ger t anders als auf die vom Hersteller vorgesehene Weise verwendet wird k nnen die Sicherheitseigenschaften des Ger ts beeintr chtigt werden QuickGene 810 User s Guide Rev 1 3 Informationen zur Installation SS AN WARNING e Stellen Sie das QuickGene 810 nicht an einem Ort auf an dem es Spritzwasser ausgesetzt ist Dadurch k nnte es zu einem Ausfall des Ger ts zu einem Brand oder Stromschlag kommen e Wenn Sie den Aufstellungsort des QuickGene 810 ndern stellen Sie sicher dass vorher der Netzstecker gezogen ist Achten Sie bei einer Standortver nderung des QuickGene 810 darauf wohin Sie treten Wenn das Netzkabel besch digt wird kann es zu einem Ausfall des Ger ts zu ein
53. ic environment this product may cause radio interference in which case the user may be required to take adequate measures This Class A digital apparatus complies whith Canadian ICES 003 Cet appareil mum rique de la class A est comforme la nomal NMB 003 du Canada 18 QuickGene 810 User s Guide Rev 1 3 3 Operation 3 Operation This chapter explains how to turn the power on and off and explains the extraction process i Wear gloves and a mask during any operation with a Biohazard risk BIOHAZARD Do not place any objects containing liquid such as a tray on the AN CAUTION QuickGene 810 If the liquid spills it may damage the operation panel Important During the operation put on appropriate gloves and a mask depending on the operation In the operation from preparing samples to extraction completion be careful not to contaminate the samples with sweat saliva etc 3 3 1 Turn the Power ON and OFF This section explains how to turn the power of QuickGene 810 on and off m Turning on the power 1 Check the power cable connection and the earth leakage breaker e Check whether the power cable is connected to the QuickGene 8 10 and the outlet If not connect it e Check that the earth leakage breaker has not tripped When the breaker is OK you can see I If the breaker has tripped you will see O If you see O contact your local Breaker checkpoint Outlet representa
54. keine Haftung f r die Verletzung von Copyright oder anderen Rechten Dritter die aus der Verwendung jeglicher Informationen dieser Anleitung entstehen k nnen e Fuji Photo Film Co Ltd bernimmt keine Haftung f r Ger te Ausf lle oder Besch digungen die durch Installation Standortver nderung Ver nderungen Wartung oder Instandsetzung entstehen sofern diese nicht durch Fuji Photo Film Co Ltd oder von Fuji Photo Film Co Ltd genannte Auftragnehmer durchgef hrt wurden e Fuji Photo Film Co Ltd bernimmt keine Haftung f r Ausf lle und Besch digungen unserer Produkte die durch Produkte von Drittherstellern verursacht wurden sofern diese Produkte nicht von Fuji Photo Film Co Ltd geliefert wurden Fuji Photo Film Co Ltd bernimmt keine Haftung f r Gerate Ausfalle und Besch digungen die durch Ver nderungen Wartung Instandsetzung usw unter Verwendung von Ersatzteilen verursacht wurden die nicht von Fuji Photo Film Co Ltd angegebene Originalteile sind e Fuji Photo Film Co Ltd bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die auf die Nichtbeachtung der in dieser Anleitung beschriebenen Vorsichtsma regeln und Betriebsverfahren zur ckzuf hren sind Fuji Photo Film Co Ltd bernimmt keine Haftung f r Ger te Ausf lle und Besch digungen die auf den Betrieb des Ger ts unter Bedingungen zur ckzuf hren ist die den in dieser Anleitung beschriebenen Betriebsanforderungen
55. local representative Turn off the power at once and remove the power cable from the outlet Then contact your local representative QuickGene 810 User s Guide Rev 1 3 45 6 Troubleshooting Phenomenon Cause Appropriate action Wash buffer or elution buffer does not flow through the nozzle Isthe Pump Tube completely attached to the joint joint Connect the Pump Tube completely to the Is there buffer in the conical tube When the conical tube is empty put the buffer into the tube Has the tube connecting the device to the pump become unfastened from the device Contact your local representative 6 3 Error Messages When an error message appears on the LCD perform the appropriate action written in the table and contact your local representative if necessary The error messages displayed on the LCD are as follows Message Contents Appropriate action MTERCR Carrier motor or air pump has failed Contact your local MTER HD The nozzle head motor has failed representative The message CR MT ER TB Table motor or lift motor has failed HD TB and WS may appear atthe same MTER WS Wash buffer pump or elution buffer pump is not time depending on failure normal type POWER ALARM The power voltage status is not normal EEPROM ER U19 EEPROM ER U18 EEPROM ER ALL EEPROM ER CSUM The program in the QuickGene 810 i
56. me press the button TED Ir RENAME COMPLE The LCD display changes to the RENAME MODE Y gt A without saving the new name e To cancel the new name press the RENAME MODE Y gt A STOP or RESET button Exit RENAME mode and change The setup mode ends without saving the to USER mode new name and the current extraction mode will be displayed QuickGene 810 User s Guide rev 1 3 6 Troubleshooting 6 Troubleshooting This chapter describes the procedures that should be taken when you suspect device failures and error messages shown on the LCD 6 1 Extraction Failure If samples cannot be extracted successfully check the parameter settings If an inappropriate parameter value has been set it will cause a buffer spill or sample extraction error Be careful when changing the values When changing parameters follow the instructions of the kit s Handbook For details of parameter setting refer to Chapter 5 How to Set Parameters gt page 37 Ifthe problems cannot be solved contact your local representative 6 2 Typical Failure Check the following items before contacting your local representative Phenomenon Cause Appropriate action The QuickGene 810 does not start after turning on the power Is the power plug connected to the outlet Connect the power plug to the outlet completely When you opened the package the circuit breaker had already been tripped There may be a current
57. n refer to ll Discharge page 20 2 Repeat the discharge operation 4 times check the tube is no ethanol remaining 3 Dispose of the waste in the Discharge Tray 4 Turn off the power m Tasks required when the QuickGene 810 is used again after one week or more When using the QuickGene 810 again perform the following procedures 1 Put 7ml of sterile distilled water in both conical tubes for wash buffer and elution buffer and perform the discharge operation Perform the discharge operation 4 times For the discharge operation refer to ll Discharge page 20 2 Dispose of the waste in the Discharge Tray 3 Perform the extraction operation as usual QuickGene 810 User s Guide Rev 1 3 31 4 Maintenance 4 3 Cleaning the QuickGene 810 32 wARNING This section explains how to clean the QuickGene 810 Before starting the maintenance work turn off the power of QuickGene 810 and disconnect the power plug from the outlet Otherwise it may cause an electric shock Pressure Head Note When cleaning the QuickGene 810 wear gloves The following describes the parts to clean and cleaning methods Carrier Rail Note Do not clean the carrier rail QuickGene 810 e outside QuickGene 810 inside
58. n emplacement loign d objets qui d gagent un champ magn tique fort moteurs transformateurs TV haut parleurs aimants etc Si le QuickGene 810 est proximit d un type quelconque de champ magn tique il risque dy avoir un dysfonctionnement Limitations d utilisation du produit Fuji Photo Film Co Ltd n assume aucune responsabilit de r sultats quel que soit l usage voulu Il est de la responsabilit de l utilisateur de valider la performance des kits QuickGene et du QuickGene 810 dans tous les cas d utilisation Le QuickGene 810 est un appareil uniquement destin la recherche et l tude Lors de l utilisation de QuickGene 8 10 lisez d abord ce manuel enti rement et respectez les r glements de gestion de risque de votre lieu de travail laboratoire QuickGene 810 User s Guide Rev 1 3 Sicherheitsinformationen In diesem Kapitel sind die Sicherheitsma regeln beschrieben Um Verletzungen zu vermeiden befolgen Sie bitte die Bedienungsrichtlinien f r QuickGene 810 auf den folgenden Seiten Informationen zum Ger t Bei Missachtung der folgenden Warnhinweise besteht Brand und AN WARNING Stromschlaggefahr Verwenden Sie ein Netzkabel das den geltenden Bestimmungen lhres Landes entspricht oder wenden Sie sich an Ihren rtlichen H ndler Schlie en Sie das Netzkabel an eine geerdete Steckdose an Fassen Sie den Netzstecker nicht mit nassen H nden an Betreiben Sie das QuickGene 810
59. n has not started yet A total of seven modes can be memorized PROCESSING Working the extraction program The extraction operation is completed The extraction result will be COMPLETED displayed after showing this message for one second Temporarily stops This message is displayed in the following cases e While waiting for the additional process START SW gt RESTART When opening the front cover during the extraction process to interrupt it and then closing the cover When pressing the STOP button during the extraction process This shows the extraction result indicates one of the following marks and meanings 172732425267778 v check Successfully extracted hyphen Extraction failure Cartridge is clogged _ underscore No cartridge or No sample QuickGene 810 User s Guide Rev 1 3 17 2 Basic Functions 2 5 Main Specifications The following describes the main specifications of the QuickGene 810 m Main specifications Items Specifications Model name QuickGene 810 Dimensions 448mm W x 332mm D x 398mm H Weight 21kg Rated input voltage AC100V 10 to AC240V 10 Power frequency 50 60 Hz Current consumption 0 7A to 0 4A Phase Single with 3P grounding pole Range of temperature and Temperature 15 30 C relative humidity Humidity 30 80 RH non condensing Noises 70 dB A max when measured at the position of 0 25 me
60. n results 1v2v3v4A 5_6_7_8_ The numerics are cartridge numbers Number 1 to 3 Extracted successfully Number 4 Extraction failed Number 5 to 8 There were no cartridges 12 Press the START button The QuickGene 810 automatically starts the extraction program LCD displays PROCESSING during extracting Extraction progress can be checked by each lamp LED Note Do not open the front cover during the extraction process while PROCESSING is displayed in the LCD If the front cover is opened the extraction process will be halted To continue the process refer to 3 5 Operations to Restart Program from Pause page 28 To quit the process refer to 3 6 Operations to Stop Extraction Process page 29 e In some extraction modes operations are required during processing For details refer to TBD 13 Two beeps with all the three lamps BINDING WASHING ELUTION blinking indicate the process completion The LCD displays the extraction results Display Meaning v check Successfully extracted Extraction failure Cartridge is hyphen clogged _ underscore No cartridge or No sample QuickGene 810 User s Guide Rev 1 3 3 Operation m Optional Operation Procedure WASH2 program When you chose optional operation mode such as xxx PLUS mode a program added WASH 2 process see 5 How to Set Parameters page 37 QuickGene 810 pauses after firs
61. n the setup mode changed parameters are saved and quit setup mode 8 STOP Halts the program of QuickGene 810 In the setup mode changed parameters are not saved and quit setup mode Can be used in the following cases to abort the program When pressing the STOP button during the extraction process 9 RESET When the front cover is opened during extraction process and the process is paused In the setup mode changed parameters are not saved and quit setup mode QuickGene 810 User s Guide Rev 1 3 7 1 Installation No Name Function Discharges the old wash buffer and elution buffer remaining in the tubes 10 DISCHARGE Discharges the remaining air from the tubes and fills the tubes with new wash buffer and elution buffer Switches the extraction modes of QuickGene 810 The button toggles the operation mode DNA WHOLE BLOOD RNA CELL RNA CELL PLUS DNA TISSUE RNA TISSUE RNA TISSUE PLUS PLASMID Extraction mode names can be changed 11 MODE Moves the carrier from side to side Each time the switch is pressed the carrier 12 CR MOVE moves from side to side Can be used while the front cover is closed Can only be used at setup mode 13 A Increments the setting value of the item displayed in the LCD at setup mode Pressing Y and A simultaneously starts the setup Decrements the setting value of the item displayed in the LCD at setup mode Can only be used at setup mode 14 Y 8 QuickG
62. nditions de fonctionnement alimentation environnement de l installation etc d crites dans ce manuel Fuji Photo Film Co Ltd ne peut tre tenu responsable des d faillances ou des dommages de l appareil dus des catastrophes naturelles telles qu un incendie un tremblement de terre des inondations ou des clairs e Aucune partie de ce manuel ne peut tre reproduite sans l autorisation de Fuji Photo Film Co Ltd Marques Les noms de compagnies et de produits sont des marques ou des marques d pos es de leurs propri taires respectifs Tous droits r serv s Copyright Fuji Photo Film Co Ltd 2005 vi Conventions Ce manuel contient des informations importantes concernant l utilisation en toute s curit du QuickGene 810 Lisez attentivement ces informations avant d utiliser et de faire fonctionner le QuickGene 810 Les significations des symboles de s curit utilis s sont les suivantes WARNING signale des conditions de dangerosit qui si elles n taient AN WARNING pas vit es pourraient entra ner la mort ou des blessures graves BIOHAZARD indique l exposition un agent biologique ou une situation qui peut tre infectieuse ou nocive BIOHAZARD CAUTION signale un danger possible qui s il n tait pas vit pourrait A CAUTION entra ner des blessures corporelles mineures ou l g res CAUTION pourrait aussi indiquer un risque de dommage mat riel Impor
63. of the QuickGene 810 Read this information carefully before using the QuickGene 810 and start operation The meanings of safety precaution marks are as follows WARNING indicates a dangerous condition that may lead to death or AN WARNING serious injury BIOHAZARD indicates exposure to a biological agent or condition that may be infectious or harmful BIOHAZARD CAUTION indicates the possibility of danger that may lead to minor or A CAUTION moderate personal injury CAUTION may also indicate an accident of property damage Important Important shows the important notes for usage as well as prohibited actions Note Note indicates the notes procedures that should be obeyed and supplementary information for use QuickGene 810 User s Guide Rev 1 3 Dil 5 QuickGene 810 QuickGene 810 DNA RNA QuickGene 810
64. of the number on the Tube Holder Cartridge a a 7 Check the location of the tubes and cartridges Cartridge Holder Check that cartridges collection tubes and waste tubes are inserted in correct position as shown in the OO 80000 Am 1 2 3 4 5 6 7 8 If they are not in order the ES N 0 N N N buffer and or lysate may 1 2 3 4 5 6 7 8 spill on the Holder Stand O0OC O0OO causing a biohazard risk OK OK OK NG Tube Holder BIOHAZARD 8 Put Iysate samples into each cartridge Wispense Lysate samples Use a pipette to transfer the lysate samples into the cartridges In this operation do not to touch the cartridges and the Cartridge Holder Note If any lysate is left on the edge of the cartridge wipe it with a Kimwipe or paper tissue 9 Set the Holder Carriage into the QuickGene 810 Set in the notch TZ After setting it check the following NS e Check that the Cartridge Holder is firmly in place Check thatthe Tube Holder rests firmly on the El Check that the two metal shelf and two pins are placed in the pins are aligned holes the holes Zero Setup the Holder Carriage gt page 13 10 Close the front cover 11 Select an extraction mode Press the MODE button several times until the target extraction mode appears QuickGene 810 User s Guide Rev 1 3 23 3 Operation 24 Display of extraction results v and __are extractio
65. ondensing and may have a negative influence on the sample result A location far from water taps water heaters humidifiers air conditioners and heaters Avoid areas of high temperature and high humidity or low temperature and low humidity A location far from objects which generate strong magnetic fields motors transformers TV audio speakers magnets etc Bringing the QuickGene 810 close to any type of magnetic field may cause a malfunction Product Use Limitations Fuji Photo Film Co Ltd assumes no liability for results regardless of the intended use It is the user s responsibility to validate the performance of QuickGene kits and QuickGene 810 for any case of use The QuickGene 810 is a device for research and study only When you use the QuickGene 810 read this manual thoroughly and follow the safety management regulations of your facilities laboratory QuickGene 810 User s Guide Rev 1 3 QuickGene 810 u JN WARNING
66. p is running operation ofthe device will not be paused until the process for one cartridge is completed After that the extraction process can be resumed from the operation panel To resume the operation after opening the front cover during extraction operation perform the following procedures To quit the program refer to 3 6 Operations to Stop Extraction Process page 29 Note When the front cover is opened do not remove the cartridges or tubes in the QuickGene 810 Also do not move the pressure head Extraction process restarts from the position where the process was paused Operations such as discharge may be performed even without cartridges or tubes which may result in liquid spill 1 Close the front cover 2 Press the START button when START SW RESTART is displayed on the LCD QuickGene 810 will restart from the position where the program is paused Restarting operations vary depending on the timing when the front cover is opened when the operation is paused The following shows the timing when the front cover is opened and the restart operations Process status Operations when opening the front cover Operations at restart During initialization All operations stop The pressure head returns to the original position then the operations restart from the paused position During binding All operations stop e The pressure head returns to the original position then moves
67. rmed by parties other than Fuji Photo Film Co Ltd or agencies specified by Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd assumes no liability for failures or damages of our products due to third party products other than products delivered by Fuji Photo Film Co Ltd Fuji Photo Film Co Ltd assumes no liability for device failures or damages due to remodeling maintenance repairs etc using repair parts other than genuine parts specified by Fuji Photo Film Co Ltd e Fuji Photo Film Co Ltd assumes no liability for device failures or damages due to failure to observe the precautions and operation procedures described in this manual Fuji Photo Film Co Ltd assumes no liability for device failures or damages due to using the device in an environment that does not meet the operational requirements power installation environment etc described in this manual e Fuji Photo Film Co Ltd assumes no liability for device failures or damages due to natural disasters such as fire earthquakes floods or lightning No part of the contents of this manual may be reproduced without permission from Fuji Photo Film Co Ltd m Trademarks Company names and product names are trademarks or registered trademarks of their respective owners All Rights Reserved Copyright Fuji Photo Film Co Ltd 2005 QuickGene 810 User s Guide Rev 1 3 Notations This manual contains important information regarding the safe use
68. s not normal LIFT POS ERR Lift position is not normal WD PUMP ERR Wash buffer pump position is not normal CD PUMP ERR elution buffer pump position is not normal PRESSURE ERR Pressure sensor is not normal Contact your local representative COVER OPEN The front cover is open Close the front cover after the necessary operation STOP SW PUSHED The STOP switch is pressed and the QuickGene 810 operation is stopped Press the RESET switch 6 4 When the Buffer is Contaminated When the buffer wash buffer or elution buffer is contaminated wash the tube well following the procedure 1 Put 7ml of ethanol in the conical tube and perform the discharge operation 4 times For the discharge operation refer to gt Ill Discharge page 20 2 Put 7ml of sterile distilled water in each conical tube and repeat the discharge operation 4 times QuickGene 810 User s Guide rev 1 3 Appendix A A 1 Usable Power Supply Cords Model Input Connector Cord Attachment plug cap North 100 120 IEC60320 C 22 Type SJT No 18 AWG NEMA 5 15P America Vac Rated 10A 125Vac Min parallel blade lt 1 gt UL CSA Approved 3 Conductors Single Rated 10A 125Vac lt 2 gt phase 2 current carrying UL CSA Approved conductors amp ground SP UL CSA Approved Er 200 240 IEC60320 C 22 Type SJT No 18 AWG NEMA 6 15P Vac Rated 10A 250Vac Min tand
69. schlaggefahr Verwenden Sie ein Netzkabel das den geltenden Bestimmungen lhres Landes entspricht oder wenden Sie sich an Ihren rtlichen H ndler Important Refer to A 1 Usable Power Supply Cords page 47 for details about the power cables that can be used in each country Outlet 1 Connect one of the plugs of the power cable to the inlet of QuickGene 810 Power cable LJ QuickGene 810 Rear 2 Connect the other plug of power cable to the outlet Connect to a grounded AN WARNING outlet to reach the power source QuickGene 810 User s Guide Rev 13 9 1 Installation 10 E Setup the Pump Tube The following explains how to setup the Pump Tube Note When setting the Pump Tube wear gloves Power switch on 1 Turn the power on Press the 1 side of the switch When the QuickGene 810 has been initialized the LCD displays DNA WHOLE BLOOD this name can be changed It takes about a minute 2 Press the DISCHARGE button Press the DISCHARGE button This operation makes the pump rotate to the position for ease to setup pump tubes 3 Turn off the power Press the O side of the switch 4 Open the front cover Hold the handle and open the front cover until the upper limit is reached The front cover will not stay ZN CAUTION on the way to the upper limit If you release the front cover while
70. schnell aufgeheizt wird oder das QuickGene 810 aus einem kalten in einen warmen Raum transportiert wird kann im Ger t Kondensation auftreten die das Probenergebnis beeintr chtigen kann Gen gend Abstand von Wasserh hnen Warmwasserbereitern Luftbefeuchtern Klimaanlagen und Heizger ten Vermeiden Sie Bereiche mit hoher Temperatur und hoher Luftfeuchtigkeit oder niedriger Temperatur und niedriger Luftfeuchtigkeit Gen gend Abstand von Gegenst nden die starke Magnetfelder erzeugen Elektromotoren Transformatoren Fernsehger te Lautsprecher Magneten etc Wenn das QuickGene 810 in die N he irgendwelcher Magnetfelder gebracht wird f hrt dies zu einer Fehlfunktion Einschr nkungen der Produktverwendung Fuji Photo Film Co Ltd bernimmt keine Haftung f r die Ergebnisse unabh ngig vom Verwendungszweck Es liegt in der Verantwortung des Anwenders die Eignung der QuickGene Kits und des QuickGene 810 f r die jeweils vorgesehene Anwendung zu berpr fen Das QuickGene 810 ist nur f r den Einsatz in Forschung und Lehre vorgesehen Wenn Sie das QuickGene 810 einsetzen lesen Sie diese Anleitung sorgf ltig durch und befolgen Sie die Sicherheitsbestimmungen Ihrer Einrichtung Labor QuickGene 810 User s Guide Rev 1 3 xvii xviii QuickGene 810 User s Guide Rev 1 3 Contents 1 Installation 1 1 Installation and Use Conditions 1 1 2 Checking the QuickGene 810 and Accessories 3 1 3 Remove the Packaging M
71. sporting QuickGene 810 The front cover will not stay on the way to the upper limit If you release A CAUTION the front cover while opening it it will drop suddenly and may cause injury or device damage 4 QuickGene 810 User s Guide Rev 13 1 Installation 1 4 Overview he QuickGene 810 Int feach part 10n O he name and funct ins t 1 la 15 section exp Th m Front and side view Operation Panel Front Cover Handle Power Swtich m Rear view 090909090909090909090909090909090909090 0900090909090009000909090900090009000 0909020902090 9090900090 020909090 90009000 0900090900090009000900000000090009000 090909090909090909090909090909090909090 0309090909090903090909090909090909090 090909090909090909090909090909090909090 09090909090909090902909090909090909080 090909090909090909090909090909090909090 0900090909090009000909090900090009000 09090909020909090 9000900090 909090009000 0909090909090909090209090909090909090 090909090909090909090909090909090909090 0309090909090903090909090909090909090 090909090909090909090909030909090909090 0909090909090909090909090909090909090 090909090909090909090909090909090909090 0909090909090909090909090909090909090 030909090909090309090909090903090909090 0909090909090909090909090909090909090 090909090909090909090909090909090909090 108 080909080
72. surge or electric leak caused by failure of the QuickGene 810 Contact your local representative The extraction has stopped in the middle of the operation Did you open the front cover during the operation elt sometimes stops for time adjustment during extraction work During extraction work While the operation panel displays PROCESSING do not open the front cover If you opened the front cover during the operation and it stopped the extraction work refer to 3 5 Operations to Restart Program from Pause page 28 The extraction work has stopped because the wash buffer and elution buffer will not flow through the nozzle Did you perform the discharge operation Do not forget to press the DISCHARGE button after putting the wash buffer and elution buffer into the conical tubes The preprocessed sample that has been dispensed in the cartridge does not steadily drop into the collection tube and the waste tube Is the cartridge inserted correctly into the Cartridge Holder Insert the cartridges completely into the Cartridge Holder Set the collection tube and the waste tube corresponding to the cartridge inserting point The position of collection Tube Holder table may be located in wrong place Contact your local representative The QuickGene 810 emits an abnormal noise The QuickGene 810 emits an abnormal smell A failure may be suspected Contact your
73. t WASH and LCD displays START SW gt RESTART Then the following steps are continued the program 1 Open the front cover Open the front cover after LCD displays START SW gt RESTART with 8 beeps The front cover will not stay A CAUTION on the way to the upper limit If you release the front cover while opening it it may cause injury or device damage 2 Take the Holder Carriage out of the QuickGene 810 Grip the handles lift the Holder Carriage up and then take it out N Grip handles and lift to take out 3 Perform the additional operations For example dispense a reagent into the cartridges 4 Set the Holder Carriage into the QuickGene 810 After setting it check the following e Check that the Cartridge Holder is firmly in place e Check that the Tube Holder rests firmly on the metal shelf and two pins are placed in the holes Setup the Holder Carriage page 13 SS Holder Carriage m T Im yy 5 Close the front cover 6 Press the START button QuickGene 810 re starts the program from the paused point There may be no noise for a while depending on the extraction mode or the settings but the LCD should show a PROCESSING QuickGene 810 User s Guide Rev 1 3 25 3 Operation m Operations after the completion 1 Open the front cover The front cover will not stay Z CAUTION on the way to the
74. t wichtige Bedienungshinweise sowie zu unterlassende Vorgehensweisen Note Note kennzeichnet Hinweise Vorgehensweisen die befolgt werden sollten und zus tzliche Informationen zur Bedienung QuickGene 810 User s Guide Rev 1 3 Safety Information This chapter describes safety precautions For your safety and that of others follow the guidelines provided in the following pages concerning the use ofthe QuickGene 810 About Instrument Br AN eee the following notations may lead to fire or electric shock WARNING Use a power cable that meets your country s standard or contact your local distributor Connect to a grounded outlet to reach the power source Do not touch the plug with wet hands Do not use the QuickGene 810 with voltage other than the voltage specified on the device Do not overload the power outlet with too many devices Donotuse the QuickGene 810 with a damaged power plug or a loose socket When dust is on the prongs of the power plug or the plug socket clean with a dry cloth When you disconnect the plug from the outlet be sure to hold the power plug itself Do not pull the power cable For maintenance disconnect the power plug from the outlet Do not touch the power plug when you hear the crash of thunder Do not pour any liquid on the QuickGene 810 Do not place any objects containing liquid on the QuickGene 810 Doing so may cause a device failure fire or electric shock Inth
75. tant Important signale des remarques importantes concernant l utilisation et les actions interdites Note Note les remarques concernent les proc dures respecter elles fournissent aussi des informations compl mentaires QuickGene 810 User s Guide Rev 1 3 Einf hrung Wir danken Ihnen f r den Kauf des Nukleins ureisolierungssystems QuickGene 810 im Folgenden als QuickGene 810 bezeichnet Mit diesem Ger t kann durch einfache Bedienung eine DNA und RNA Extraktion durchgef hrt werden Diese Anleitung enth lt wichtige Informationen zur sicheren Verwendung von QuickGene 810 Lesen Sie die Anleitung vor der Verwendung sorgf ltig durch Bewahren Sie diese Anleitung nach dem Lesen zum sp teren Nachschlagen auf Umgang mit infekti sen Stoffen Befolgen Sie bei der Handhabung oder Entsorgung von infekti sen Abf llen die Laborrichtlinien sowie die geltenden Gesetze und Vorschriften und f hren Sie die Verbrennung Einschmelzung Sterilisation und oder Desinfektion entsprechend durch Wenn Sie die Entsorgung durch Dritte ausf hren lassen beauftragen Sie zugelassene Entsorgungsfirmen f r industriellen Sonderm ll und stellen Sie ihnen Universal biological die relevanten Informationen zur Sonderm ll Abfallbehandlung zur Verf gung hazard symbol m Ausschlussklauseln Der Inhalt dieser Anleitung kann jederzeit ohne Vorank ndigung ge ndert werden e Fuji Photo Film Co Ltd bernimmt
76. ters from the equipment surface and 1 2 meters from the floor Ger uschentwicklung Max 70 dB A bei Messung in einer Entfernung von 0 25 m vom Ger t und einer H he von 1 2 m ber dem Boden Operation panel display LCD 16 characters x 1 line Samples Max 8 samples can be processed simultaneously Sit Indoor use only m Environmental conditions EMC FCC Rule Part15b ClassA EN61326 ClassA EN61000 3 2 EN61000 3 3 Safety UL61010A 1 CSA C22 2 No 1010 EN61010 1 IEC61010 1 CE LV directive 73 23 EEC EMC directive 89 336 EEC FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the users will be required to correct the interference at their own expense Warning This is a Class A product of Electromagnetic Interference EMI standard In a domest
77. the MODE button several times until the target extraction mode appears 2 Press the A W buttons simultaneously Setup mode starts 3 Hold down the MODE or DISCHARGE button until the following message is displayed on the LCD EXPERT MODE Y gt A 4 Press the A button Change the mode to the EXPERT mode Settings of parameters in EXPERT mode can be changed with the same procedures as in the normal setup mode For details refer to Il How to change Parameters page 39 42 QuickGene 810 User s Guide rev 1 3 5 How to Set Parameters 5 4 Changing Extraction Mode Names Extraction mode names displayed on the LCD can be changed Renaming is performed in RENAME mode Important In RENAME mode only extraction mode names are changed without changing parameter values of the corresponding modes The following names can be used for extraction mode name change Fos Extraction mode name Rte Extraction mode name 1 DNA WHOLE BLOOD Default 19 DNA PLANT 2 RNA CELL Default 20 RNA PLANT 3 RNA CELL PLUS Default 21 DNA VIRUS 4 DNA TISSUE Default 22 RNA VIRUS 5 RNA TISSUE Default 23 SWAB 6 RNA TISSUE PLUS Default 24 STOOL 7 PLASMID Default 25 PCR PURIFY 8 ISOLATE A 26 GEL EXTRACTION 9 ISOLATE B 27 REACTION CLEANUP 10 ISOLATE C 28 DYE CLEANUP 11 ISOLATE D 29 RNA BACTERIA 12 ISOLATE E 30 DNA BACTERIA 13 ISOLATE F 31 BACTERIA PLUS
78. tive Connect to a grounded Powercable QuickGene 810 Rear AN WARNING outlet to reach the power source 2 Press the I side of the switch Just after the power is turned on the LCD displays the software version for a few seconds then changes the display to WAIT A MOMENT LCD displays extraction mode name when the initialization has been completed It takes about a minute for the initialize eo Power switch eo m Turning off the power 1 Press the O side of the switch QuickGene 810 User s Guide Rev 1 3 19 3 Operation 3 2 Preparation This section explains the preparation of the QuickGene 810 for an extraction process The following procedures must be performed as preparation ofthe QuickGene 810 m Preparation items Before a test run or extraction operation perform the following e Check all the parts are installed 1 5 Setup Parts page 9 Prepare the following Gloves Mask Conical Tubes 1 set Collection Tube CT provided with the kit Waste Tube WT provided with the kit Cartridge CA provided with the kit Buffer wash buffer elution buffer provided with the kit For a test run use sterile distilled water Lysate Samples For a test run use sterile distilled water For the preparation of samples refer to each kit s handbook Conical tubes are used for elution buffer and wash buffer Refer to 1 5 Set
79. tton not the START button to stop the process The mid process extraction results will be displayed on the LCD 4 Perform the operation at extraction completion Operations after the completion page 26 ER 3 7 Quick Guide When performing an extraction operation check and follow the following Execute discharge first Please execute discharge first This operation discharges the old wash buffer elution buffer and air remaining in the tubes and refills new wash buffer and elution buffer After the discharge operation dispose of the liquid collected in the Discharge Tray MDischarge page 20 Follow the setting order and set them correctly When setting the Tube Holder and Cartridge Holder into the Holder Carriage follow the steps below to set them correctly gt Operations before the start page 21 Failure to do so may cause the sample to spill DSet tubes in the Tube Holder then set 2 Set cartridges the Tube Holder into the Holder Carriage Cartridges Dispense lysate Dispense Iysate samples pins are aligned the holes QuickGene 810 User s Guide Rev 1 3 29 3 Operation Set cartridges collection tubes and waste tubes in the correct positions Cartridge Holder Insert the cartridges and tubes starting from the left in If they are not in order a spill of buffer and or 1 2 3 4 5 6 7 8 r t dissolved sample may occur This results in an e
80. up Parts gt page 9 m Discharge 1 Check the QuickGene 810 has been cleaned For clean up procedure refer to 4 3 Cleaning the QuickGene 810 page 32 and 4 4 Cleaning the Parts page 33 2 Turn on the power of QuickGene 810 3 1 Turn the Power ON and OFF page 19 3 Open the front cover Take the handle and open the front cover as shown in the figure The front cover will not stay A CAUTION on the way to the upper limit If you release the front cover while opening it it may cause injury or device damage 4 Check that the Discharge Tray is set on the Buffer Stand If it is not set set it Setup the Tube Cap gt page 13 20 QuickGene 810 User s Guide Rev 1 3 3 Operation 5 Put buffers wash buffer and elution buffer into conical tubes Elution buffer Wash buffer Large conical tube wash buffer Small conical tube elution buffer a Important Buffers can be used for a day in the conical tube inside the QuickGene 810 Small conical tube Large conical tube 6 Set the conical tubes which were prepared in 4 into the Buffer Stand and put Tube Caps on the conical tubes Note Do not touch the inner tube of Tube Cap In the case of contamination please refer to 6 4 When the Buffer is Contaminated page 46 Tube Cap Blug Small conical tube en ct Large conical tube u 0 Ce J 7 Close the front cover 8
81. upper limit If you release the front cover while opening it it may cause injury or device damage 2 Take the Tube Holder out of the QuickGene 810 Note Take the handle of the Tube Holder After taking off the Holder Carriage from the QuickGene 810 the Tube Holder cannot be taken out of the the Holder Carriage Therefore take only the Tube Holder out of the QuickGene 810 elf you took off the Holder Carriage from the QuickGene 810 take off the Cartridge Holder first and then take out the Tube Holder 3 Take the collection tubes out Put caps on the collection tubes Then take the collection tubes out Note Check the caps are put on the tubes completely 4 Take the waste tubes out Dispose of the waste tubes according to appropriate laws and rules Dispose of the infectious wastes according to your biological safety manuals BIOHAZARD Waste Container 26 QuickGene 810 User s Guide Rev 1 3 3 Operation 5 Take the Holder Carriage out of the QuickGene 810 Grip the handles lift the Holder Carriage up and then take it out Note When the carrier is in the center and it is difficult to remove the Holder Carriage close the front cover and press the CR MOVE button Grip handles and lift to take out 6 Remove the Cartridge Holder from the Holder Carriage and dispose of the cartridges To drop down the cartridges sli
82. x personnes au moins sont n cessaires Vous risquez autrement de vous faire mal au dos ou aux mains du fait du poids de l appareil plus de 20 kg Pour une utilisation correcte et s re du QuickGene 810 installez le dans un emplacement qui r pond aux conditions suivantes Un emplacement proximit d une alimentation lectrique Utilisez une prise CA100 10 CA240V 10 50Hz ou 60Hz Un emplacement o la temp rature est situ e entre 15 30 C et ou humidit relative est comprise entre 30 80 RH sans condensation Un emplacement qui peut soutenir le poids du QuickGene 810 21 kg qui est plane stable et sans vibration Un emplacement loign des rayons directs du soleil Si n cessaire bloquez le soleil avec des rideaux ou des stores Un emplacement correctement a r et sans poussi re Un emplacement o la temp rature ne varie pas brutalement Si vous r chauffez brusquement une pi ce fra che ou si vous d placez le QuickGene 810 d une pi ce o la temp rature est basse une autre o il fait plus chaud de la condensation risque de se produire l int rieur de l appareil et peut avoir une influence adverse sur le r sultat de l chantillon Un emplacement loign de robinets d eau de bouilloires d humidificateurs d appareils d air conditionn et de chauffage Evitez les emplacements temp rature lev e et forte humidit ou basse temp rature et faible humidit U
83. xtraction failure and buffer and or dissolved sample 29000000 00000 OK OK OK NG Tube Holder 30 QuickGene 810 User s Guide Rev 1 3 4 Maintenance 4 Maintenance This chapter describes how to maintain the QuickGene 810 When cleaning the QuickGene 810 wear gloves BIOHAZARD 4 1 Before Using the QuickGene 810 Before using the QuickGene 810 perform the following tasks Keep clean inside the instrument and parts Supply Replace buffers wash buffer and elution buffer The buffers must be used within one day from when they are set in the QuickGene 810 Execute the discharge operation when replacing the buffers in the tubes in QuickGene 810 4 2 When the QuickGene 810 is left for a Week or More Even after completing the operation and turning off the power there are buffers wash buffer elution buffer remaining in the QuickGene 810 When the QuickGene 810 will not be used for a week or more the remaining buffers may become crystallized and cause a clog or damage to the tubes Discharge the remaining buffers from the QuickGene 810 and clean the inside with ethanol m Tasks required when the device is not used for a week or more Ifthe QuickGene 810 will not be used for a week or more after the operation perform the following procedures 1 Put 7ml of ethanol in both conical tubes for wash buffer and elution buffer and perform the discharge operation For the discharge operatio

Nucleic Acid Isolation System QuickGene

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