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Spectrum 100 Series User's Guide

1. eee 30 Safety Interlock uusssss 27 Sample Compartment 26 Switching on eeeeeeeeenee 54 Top Panel Controls 25 UNPACKING nnne enn 31 T Temperature 13 V Validation certificate printing sss 102 instrument validation 101 principles of USE 100 reasons for failure s 104 re printing a certificate 106 when to Recalibrate 107 W WEEE Directive 115 When to Recalibrate 107 Windows in Main Cover seen 92 Installing Replacing 91 Seal zh deteecese e ier tee depu 91 Windows Start Menu 54 118 Spectrum 100 Series User s Guide
2. eenenenne 81 Advanced Maintenance 1 neee essere eere nnn 83 Opening the Main Cover seessesseseeseennee nennen 84 Replacing the Sources seien er ierat rete Er Ese padre ce Exp RR ns 87 Replacing the Beamsplitter eeeeeeeeeeeeee nnnm 89 Installing Replacing Windows eee 91 Installing Filters in the Filterwheel eeseseeeeeemm e 93 Installing an AVI Cell in the Filterwheel Assembly 96 Replacing the Laser and Power Supply eeeeeeeenn emen 97 Instrument Performance Validation eere 99 Performance Validation Kits eeeeeeeeeeeeeen nennen 100 Principles of Use esee eere dan a eur ere ode eec ret 100 Validating Instrument Calibration esee 101 Collecting the Reference Spectra sseseseeeeene 101 Printing the Certificate of Performance Validation 102 Possible Reasons for Validation Failure seseeeesussss 104 Printing the Certificate 1 5 ceret deerit ierant eter e tagen 104 Re printing the Information on a Certificate sssssssssss 106 When Should I Recalibrate My Instrument eeeessese 107 Appendices 1 rannin nananana nennen nne nnnm anaa aas anora aai Na
3. 44 Spectrum 100 Series User s Guide The Spectrum Software works through a series of instrument performance tests Spectrum Instrument Install Wizard Test Instrument Performance Instrument Wizard Testing the performance of your instrument Performance Startup Passed o Test Performance gt Testing Performance Laser Amplitude Passed Inteferogram Passed ASTM Reference Spectra 2 Finished Wavelength Calibration In progress Background Pending SNR Pending Polystyrene Pending To view the log click View Log View Log e Start up Reviews the power up diagnostics to make sure no problems were found when the Spectrum instrument was switched on e Laser Amplitude Checks the amplitude of the laser e interferogram Checks the amplitude of the interferogram e Wavelength Calibration Performs a wavelength calibration by checking and correcting against the position of a reference peak in a polystyrene spectrum which is collected either from the internal polystyrene filter or if not fitted then by asking you to place the polystyrene test card supplied with the instrument in the sample slide e Background Collects a background spectrum and checks the beamsplitter cut off and the energy levels e SNR Collects spectra and performs a signal to noise ratio test checked against a set limit e Polystyrene If an internal polystyrene filter is not available you will be asked to insert the test card
4. 5 Insert the plastic funnel into the funnel guide and slowly fill the detector dewar with liquid nitrogen NOTE Make sure that the detector dewar is filled to the top with liquid nitrogen before selecting the detector Prevent radiation from reaching the detector by obstructing the beam path in the sample area during filling 6 Stand back and wait two minutes This nitrogen also vaporizes as the dewar continues to cool The two minute wait allows the bubbling to settle down and the pressure of the vaporizing nitrogen to dissipate 7 Continue to pour liquid nitrogen into the funnel adding a little more each time the funnel empties The funnel takes longer to empty as the dewar fills This happens after two or three more funnels of liquid nitrogen Because the dewar has now cooled the liquid nitrogen does not vaporize but instead fills the dewar 8 Remove the funnel and wait two minutes The liquid nitrogen settles down and bubbling slows 9 When the nitrogen stops bubbling refit the plug When the detector dewar has been filled it will remain at its operating temperature for approximately eight hours NOTE Cooled detector dewars require pumping down after approximately 12 months operation When the boil off rate of liquid nitrogen becomes excessive that is the liquid nitrogen level is low after 3 4 hours operation consult the nearest PerkinElmer Service Department or Agent Advanced Maintenance
5. WARNING A length of clear plastic tubing 4 mm internal diameter will be required to transfer the gas from the gas bottle to the instrument purge connectors Routine Maintenance 77 1 The tubing is connected to the instrument by a universal pipe fitting which is secured to the tubing with a nut Two sets of fittings are supplied with the instrument Assemble the fittings to the tubing as illustrated in Figure 23 Beveled end of connector Figure 23 Purge fittings 2 Tighten the nut ensuring it is not over tightened 3 Push the connector on the end of the tubing into the purge connector of the compartment to be purged Figure 24 Sample Purge 7a Instrument Purge Figure 24 Connector fitted to purge port 4 Connect the free end of the tubing to the gas regulator NOTE Ensure the regulator is set to a pressure not greater than 6 9 kPa 1 Ibf in 5 Open the gas flow valve on the regulator until a flow rate of 28 L minute 1 ft minute is achieved The instrument compartment is purged in 10 minutes The sample compartment is purged in 2 minutes 6 Once purging is complete turn off the gas supply 78 Spectrum 100 Series User s Guide 7 Disconnect the fitting from the instrument by pushing the locking clip located on the side of the purging port Figure 25 Figure 25 Disconnect the locking clip Routine Maintenance 79 Changing the External Fuse Very occasionally a fuse
6. 84 Spectrum 100 Series User s Guide Opening the Main Cover To perform most maintenance tasks you have to open the main cover of the spectrometer Switch off the mains power supply to the spectrometer wait 60 second s and disconnect the power cable before you open the cover of the spectrometer This makes sure that you are safe from electrical shock and laser radiation WARNING Do not open the main cover if the humidity is CAUTION gt 75 relative humidity for an instrument fitted with KBr optics gt 45 relative humidity for an instrument fitted with CsI optics The humidity sensitive optics will be damaged if subjected to a relative humidity in excess of the specified levels The main cover is hinged at the back of the instrument and opens from the front Figure 29 identifies the locking bolts which are on the front of the instrument Figure 29 Main cover locking bolts 1 Switch off the power supply to the spectrometer 2 Wait for 60 seconds 3 Disconnect the power cable 4 Remove the sample area cover if fitted open the cover press on the cover retaining clip and pull the cover vertically to remove it Figure 30 Advanced Maintenance 85 Sample Cover Release Clip Figure 30 Removing the sample area cover 5 Remove the fitted sampling accessory by pulling the blue release handle towards you and sliding the accessory towards you Figure 31 Sampling Accessory Release Handle
7. Scan and Instrument Setup oJ C d Multiple Sample Scan Instrument Beam Accessory Range Start 4000 00 450 00 om 1 Options Scan type Sample Scan minimized Units XT Duration Scan number 1 Scan time minutes E Spectrum Help amp Hide Back Forward Print Contents Index Search Favorites 2 About this Help 2 Help on Help IQ Welcome to Spectrum Displaying Spectra Working with Spectra Q Controlling the Instrument VL Collecting Spectra 3 sample tab The Scan tab enables you to specify the scan conditions for your spectrum or inifrferogram The scan range you use depends on the area of the spectrum you are intergpted in gt To set the scan range simply type the Start and End limits The Start is the higher wavenumber 2 Accessory tab 2 Display tab co cessories Setting Up the Instrument Adjustments Toolbox Instrument Validation 2 Routine Access Users 2 Standard Access Users Working with Files and Windows Customizing Spectrum The way the instrument is set up may change the scan range that your instrument can collect This will depend on the scan speed you have chosen the detector fitted to the instrument the beamsplitter fitted to the instrument any accessory you are using Options Using the Spectrometer 61 Atmospheric CO H O Suppression Atmo
8. 106 Spectrum 100 Series User s Guide 3 Sign the certificate Keep the spectra trmpic sp and ipvpic sp as a record of the original data for future reference NOTE The values on the certificate above are for illustration only Do not use these values for validation of your instrument Re printing the Information on a Certificate Opening a ipv file 1 Open Windows Explorer 2 Choose the ipv file you want to open 3 Display the File menu and choose Open with The Open with dialog is displayed 4 Select Other 5 Select ipvc ic exe from the ipvalid directory 6 Ensure that the Always use this program to open this file box is selected 7 Click OK The IPV Certificate printing dialog is displayed with the data from the ipv file NOTE You are now able to open the ipv file by double clicking on the file name in Windows Explorer Printing the certificate 1 Double click on the ipv file you want to print The IPV Certificate Printing dialog containing the data you saved is displayed 2 Click Print The certificate is printed NOTE The data saved in a ipv file cannot be changed Instrument Performance Validation 107 When Should I Recalibrate My Instrument When using a Fourier Transform spectrometer the error in the peak positions will be proportional to the wavenumber The calibration can be adjusted by changing the effective wavenumber of the HeNe laser If the peak position
9. instrument 45V Unpacking and Installation 37 Connecting the Spectrometer to the electrical supply The power cable for the electrical supply plugs into the rear of the instrument It has a molded socket at one end If it is necessary to fit a plug on the power cable use the wire color code below PlgPin Wire Color 100 120 V Wire Color 220 240 V Ground Earth Green or Green Yellow Green Yellow To ensure safe and satisfactory operation of the instrument it is essential that the green or green yellow ground earth wire of the power cord is connected to a ground that complies with the regulations of the local electricity supply authority or equivalent body ground circuit continuity is essential for safe operation of the equipment WARNING The Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers can operate on electricity supplies of 50 or 60 Hz and in the 100 to 230 V range without any adjustment 1 Fit the molded socket of the power cable into the electrical supply inlet Figure 11 of the instrument Electricity Supply Inlet Figure 11 Electrical Supply Inlet 2 Switch on the power at the supply 3 Switch on the instrument at the switch above the supply inlet l is on and O is off After a short diagnostic check described in Appendix 2 Instrument Self Checks on page 113 your instrument will be ready to communicate with the PC 38 Spectrum 100 Series User s Guide Instal
10. 20 are pink Figure 8 The Desiccant Indicator When desiccant packs should be changed If all three sectors of the Desiccant Indicator are pink then you must change the desiccant CAUTION The instrument optics may be fogged Do not switch the instrument either ON or OFF until all sectors are blue NOTE We recommend that you change the desiccant in the spectrometer before using it for the first time Refer to Changing the Desiccant on page 71 34 Spectrum 100 Series User s Guide Connecting up the Spectrometer Connecting to the PC NOTE To control your instrument from a PC use the crossover cable supplied To control your instrument over your network use a standard Ethernet cable not supplied 1 Plug one end of the cable into the connector port labeled p on the back of the instrument Figure 9 PC or network connection port Figure 9 PC connection port 2 Plug the other end of the cable into the network connection on your PC if you are using the crossover cable or network hub if you are using a standard Ethernet cable Do not attempt to connect a monitor to the EXT R and MICROSCOPE ports or you will cause serious damage to the CAUTION instrument when it is switched on Unpacking and Installation 35 Other connectors The communications ports for peripheral devices are illustrated in Figure 10 Figure 10 Communications ports Icons which identify the function
11. Laser from the board connector which is located under the rear of the laser assembly Be careful the nearby source housing may be hot WARNING 4 Unhook the spring and remove the two screws on the retaining bracket as shown in Figure 49 5 Remove the laser and power supply noting the way the cabling is routed 98 Spectrum 100 Series User s Guide 6 Install the new laser and power supply Ensure the laser is pushed fully home to the stop at the front of the housing Laser bracket retaining screw iG LL KJ D Orientation mark Baffle retaining screws L E S servare Dnm Figure 49 Laser and power supply installation Laser bracket retaining screw P 7 Laser spring retaining pin 7 The laser must be installed so that the small orientation mark a single engraved line is facing directly upwards This should be the same orientation as the end of the triangular warning label 8 Refit the laser retaining bracket and secure with the two securing screws 9 Secure the spring on the retaining pin 10 Reconnect the PSU Laser cable to the board connector 11 Secure the laser power supply with the two securing screws Make sure that the laser cabling is secured so that it does not get trapped in the cover 12 Close and secure the main cover steps 10 to 15 on page 86 13 Plug in and switch on the instrument Instrument Performance
12. Validation 100 Spectrum 100 Series User s Guide Performance Validation Kits The Spectrum 100N is supplied with Performance Validation kit L1180479 which contains a 35 um polystyrene reference sample and an NG11 Schott Glass reference sample For the Spectrum 100 Performance Validation kit L1365335 is available This kit contains a 1 2 mm polystyrene reference sample The Performance Validation procedure is similar to that described here but the kit includes IPV Kit User s Guide 09934028 Principles of Use The Food and Drug Administration FDA the United Kingdom Accreditation Service UKAS and the Good Laboratory Practice GLP regulations all require that calibration of instrument accuracy can be traced to a certified reference material CRM Instrument Performance Validation IPV enables you to routinely generate a record of wavenumber calibration that is traceable to a National Physical Laboratory NPL CRM Using the NIR IPV certificate printing utility you produce a certificate comparing the result for a polystyrene Traceable Reference Material TRM and your internal polystyrene Automatic Precision Validator APV against a certified polystyrene standard serial number JR95 measured by the NPL Teddington TW1 OLW UK The NPL certificate of measurement provides wavenumber values for two peaks 5669 3 cm and 4571 6 cm for resolutions of 4 cm or better The polystyrene TRM supplied with your instrument has been cal
13. cell means that it also provides correction for any sampling configuration NOTE AVI correction can only be performed if an AVI Calibration has been set up for the current sampling configuration 3091 3088 3084 4 3080 3076 3072 Figure 15 Spectra of methane at 4 cm resolution as measured top and with AVI bottom 64 Spectrum 100 Series User s Guide Look Ahead Look Ahead can be set in the Advanced section on the Instrument tab of the Scan and Instrument Setup dialog What is Look Ahead Look Ahead is a novel system where the spectrometer scans continuously and uses the properties of the measured spectrum to identify changes corresponding to sample removal sample insertion or sample change automatically This information is used to identify the scans that are being collected and to accumulate sample scans This can decrease the overall scanning time required for those samples that require scan accumulation What does Look Ahead do If Look Ahead has been enabled the spectrometer scans continuously When you request a sample scan the scan collected is compared to the scans that have already been collected If identical scans are found the number of scans requested is decreased by the number of scans found so decreasing the overall scanning time Using the Spectrometer 65 Quality Checks Quality Checks can be set in the Advanced section on the Instrument tab of the Scan and Instrument Setup
14. d Notch and key 4 Secure the window to the main cover by fitting the screws and washers and tightening the screws using the hexagonal wrench Figure 39 5 Close and secure the Main Cover steps 10 to 15 on page 86 6 Plug in and switch on the instrument Advanced Maintenance 93 Installing Filters tn the Filterwheel The following procedure describes how to install a filter in the filterwheel The same procedure should be followed when replacing a filter Due to the restricted cabling the filterwheel assembly is removed from its mounting and placed on the sample compartment baseplate It therefore may be necessary to remove the sample slide Switch off the mains power supply to the spectrometer wait 60 seconds and disconnect the power cable before you open the cover of the spectrometer This makes sure that you are safe from electrical Shock and laser radiation WARNING 1 Open the main cover of the instrument as detailed on page 84 and identify the Filterwheel Figure 40 Figure 40 Filterwheel location 2 You need the standard sample holder baseplate installed for this procedure but remove the sample holder itself by unscrewing the knurled screw Figure 41 Figure 41 Knurled screw 924 Spectrum 100 Series User s Guide 3 Remove the filterwheel assembly from its mounting by undoing and removing the two securing screws Figure 42 Figure 42 Filterwheel assembly sec
15. dialog What are Quality Checks Quality checking is a tool for less experienced IR users that identifies possible problems in the spectrum being collected and suggests ways of improving the measurement What do Quality Checks do Choosing Quality Checks displays a dialog that enables you to select the quality checks you want to perform NOTE For more information about each individual test double click on the test in the dialog When you collect your spectrum the selected tests are performed as the data is collected and the signal lights at the bottom of the Display tab ooe Show the current status There are two levels of alert from the Quality Checks Caution IR Expert has identified a problem that you may want to investigate in order to improve the quality of the spectra you are collecting Warning 0 IR Expert has identified a serious problem that you should attempt to cure before collecting further spectra If a problem is identified a dialog will be displayed that not only alerts you to the problem but also explains what the problem means how it may of occurred and gives some suggestions on how to stop the problem occurring again Quality Checks and the Multiple Sample tab If Quality Checks have been set the selected tests are performed as each spectrum is collected and a signal light green 9 amber or red 9 is placed in the Multiple Sample tabs QC column to indicate its status gt T
16. is displayed this may require login details PerkinElmer Login E Enter your user name and password User name Supervisor Password akak Change Password Enter your User name and Password if necessary then click OK 3 Select the Instrument you want to use or select Work Offline if you just want to work with data that has been collected previously without attaching to an instrument Spectrum Login Please select an instrument Instrument Work Offline x Cancel Group Supervisors Help 4 If you are using Spectrum not Spectrum ES choose whether to Activate IR Assistant IR Assistant is a simplified wizard driven interface for Spectrum spectrometers IR Assistant is included in the Spectrum application sub group and is an item on the Spectrum Help menu On its start page you can elect to Always show Assistant when Spectrum starts 5 Click OK Spectrum starts Using the Spectrometer 55 Controlling the Instrument You start scans and make adjustments to the instrument using commands in the Spectrum software NOTE The level of control you have and the complexity of the dialogs you see is determined by your user level as set on the Instrument tab of the Options dialog available from the Setup menu The information that follows shows the dialogs available to the Advanced User 1 To collect data from your spectrometer select Scan on t
17. of each of the communications ports are printed on the hinge molding directly above the communications port 36 Spectrum 100 Series User s Guide Icon Description Connector Voltages Maximum Type Currents Keyboard Connects a keyboard or Mini DIN barcode reader to the spectrometer 6 way Serial port Connects a PC to the 9 way 12V instrument using an RS232 interface D type cable This connector is only used for service diagnostics 12V Universal serial bus USB This USB lt 5V connector is not used 5V 10 Base T Ethernet connector This is Ethernet lt the standard interface between the PC and the instrument or between a Local Area Network LAN and the instrument If connecting directly from the PC to the instrument use the Category 5 UTP Cross Over cable lt 100 mA lt 100 mA lt 100 mA lt 100 mA supplied If you are connecting to your network use a standard Ethernet cable DO NOT USE THE CROSS OVER CABLE TO CONNECT TO YOUR NETWORK Attachment Unit Interface Can be 5V used to connect the instrument to a LAN 12V lt 100 mA 50 mA Right external detector module 12 V 0 65 A Connects to an external detector module Usually the detector is placed 12V in an accessory placed on the right of the instrument 0 65 A T Ui lt aN gt 0 65A 0 65 A 4A Microscope external detector module 12V Connects to a PerkinElmer infrared microscope placed on the left of the 12 V
18. removed and the external beam is selected The laser radiation is not accessible at the port when an external beam accessory is attached When an external beam accessory is removed from the instrument the internal beam path must be selected or the external beam port cover must be replaced Do not stare into any laser beam Staring into a laser beam intrabeam viewing can cause permanent damage to your eyes WARNING Radiation Emitted by the Spectrum 100N FT NIR The Spectrum 100N FT NIR quartz halogen bulb which produces the near infra red beam emits ultraviolet visible and infrared radiation The majority of this radiation is in the infrared region Do not stare into the beam produced by this bulb Measurements of the infrared radiation emitted from the sample area show that exposure limits recommended by the American Conference of Governmental Industrial Hygienists ACGIH and International Commission on Non Ionizing Radiation Protection ICNIRP will not be exceeded during normal operation Warnings and Safety Information 19 Labels When this label is attached to an instrument it means Caution risk of danger Refer to the manual to find out the nature the potential hazard and any actions that have to be taken Spectrum 100 FT IR and Spectrum 100N FT NIR laser safety labels Safety labels are fixed to the Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers in the locations shown in Figure 1 and Figure 2
19. under the following conditions Ambient temperature 15 C to 35 C Ambient relative humidity 75 maximum non condensing Unpacking and Installation 31 Unpacking the Spectrometer The spectrometer is a heavy precision instrument so two people are required for safe handling The instrument weighs approximately 34 kg unpacked 40 kg packed and has a lifting recess on either side Consult the local WARNING codes of practice issued by safety advisors before attempting to lift it Take care not to injure yourself or others or to drop the spectrometer Take great care when installing your Spectrum 100 FT IR or Spectrum 100N FT NIR spectrometer and follow the procedures described in this manual If you require assistance contact your local CAUTION PerkinElmer Service Engineer Opening the shipping container Your spectrometer is packed inside the box in a silver bag that protects it from condensation 1 First remove the manuals software box leads and so on from the box and check that all the following parts are present 100 100N Software and manuals kit L1050001 Spectrum 100 Series Getting Started Guide L1050002 Spectrum 100 100N Manuals CD L1050005 Spectrum Multimedia CD L1242701 NIR Source L1240055 Cuvette Holder Assembly L1240056 Cell Holder Assembly and disposable cells 5 If any items are missing or damaged contact your local PerkinElmer office 32 Spectrum 100 Series User s G
20. you use different spectra than those suggested above Instrument Performance Validation 103 7 Make sure that the Validation Details are correct and add any missing information NOTE The serial number of your instrument is usually printed on a silver label in the lower sample compartment area The serial number of the TRM is on the TRM sample holder 8 Click OK The information that will be printed on your Certificate of Performance Validation is displayed Perkintsmer Certiicate af Performance Validation Instrument Model Number Spectrum 100N Instrument Serial Number 50177 NPL Certified Traceable Reference Reference Material Reference Material Material TRM used in Routine Serial No JR95 Serial No PA00047 Validation Nominal nm Measured nm Measured nm 1763 9 1763 9 0 4 1763 9 2187 4 2187 4 0 4 2187 4 Nominal 7 cm 1 Measured cm 1 Measured cm 1 5669 3 5669 3 1 5 5669 3 4571 6 4571 7 1 0 4571 7 Date 18 Jan 2005 User NIR ADMIN Signed Back Save Draft Print NOTE There are two peaks with details given in both nm and cm 9 Make sure that all the details are correct Peaks found outside the tolerance of the nominal value are displayed in red If a peak is not found within 10 cm of the nominal value No Peak is displayed in red see Possible Reasons for Validation Failure on page 104 10 To save the information on the certif
21. 111 2 Remove the current sampling accessory by pulling the blue release handle under the baseplate of the accessory towards you and then sliding the accessory towards you Figure 51 Release handle Figure 51 Removing the sampling accessory 3 Install the new accessory Figure 52 by sliding it onto the ledge and pushing it into the sample compartment until it engages with the connector Figure 52 Installing the new sampling accessory 112 Spectrum 100 Series User s Guide 4 The Scan and Instrument Setup dialog automatically recognizes which accessory is installed Scan and Instrument Setup OJA 0 d amp E Scan Instrument Beam Accessory Details Name ample043 Description Comments Preview Scan Figure 53 UATR Accessory icons Spectrum 100 Series Users Guide 113 Appendix 2 Instrument Self Checks NOTE It can take the Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers up to 2 hours to equilibrate when switched on after being switched off overnight To save time we suggest that you leave the spectrometer switched on at all times e When you switch on the instrument the Instrument Display provides this sequence of messages gt Initializing Initializing Checking Program Integrity Checking Instrument Configuration Checking Source Checking Laser Checking Scan Checking Calibration Initialization Complete e When initialization is complete the last messag
22. 64 What is Look Ahead eeesssseeseeeeeee nenne nemen nennen nnn 64 What does Look Ahead dO ccccccceseeuseeecsecueeeeceeeueeueneeaueeueeuenevaueeess 64 4 Spectrum 100 Series User s Guide Qilallity GNECKS secs T ads hese cae ti SagsacaS ea caveg shade TT 65 What are Quality Checks icceseeeeeesseeeeesseseeeesaaaeeeesaaaeeeesaaaaeeseaas 65 What do Quality Checks do eeeeeenneeenm me 65 Quality Checks and the Multiple Sample tab 65 Routine Maintenance enses niei eene nnn nnn 67 Cleaning the Spectrometer risinn enirn e peer Eie ea ve Er dad 68 Cleaning the Display 1 2 5 1 erii tuos ete eco ro erii eoe eec Es rein tata 68 Moving the Spectrometer sss nemen 69 Gondensation i i eio ene a oe Dep utet Qa Pd euo ker ae Te a TE bna Ens d 69 The Desiccant Indicator in detail eeeeeeeeeennnnm nne 70 Changing the Desiccant ion eee ren ER rore tere ere e ridere 71 Resetting the desiccant change interVal ceccsesseseeeeeeeeeeeeeaneeees 72 Renewing the disposable desiccant eese 73 Installing rechargeable desiccant seeeeseese 75 Purging the Spectrometer ssesesseseseeeene nemen 76 Changing the External Fuse eseesseeseseeeeeeenennen nennen 79 Cooling the MCT Detector Spectrum 100
23. Certificate of Performance Validation Hardware and software requirements To print a certificate you will need a PC running Microsoft Windows connected to a printer with the correct print drivers installed The PC does not have to be the one connected to your instrument gt Put the disk labelled WR IPV Certificate Printing in drive a 1 From the Start menu choose Run 2 To install the program type a ipv followed by the drive you want to install the program on for example a ipv c then click OK The IPV certificate printing utility is installed in a directory named ipvalid on your selected hard drive 3 Copy the file ipvc_ic ini from the pva id directory to your Windows or WINNT directory 4 Torun the program open Explorer and double click on ipvc ic exe in the pva id directory The IPV Certificate Printing dialog is displayed IPV Certificate Printing for NIR Version 3 02 Lx IRM Spectrum Sf OK A TRMPIC SP Browse IPY Spectrum A NMPVPIC SP Validation Details Instrument Model Spectrum 100N Instrument Serial No 50177 TRM Serial No PA00047 User 1D Date 18 Jan 2005 X Cancel Copyright c 2000 PerkinElmer Inc 5 Putthe disk on which you stored the reference spectra trmpic sp and ipvpic sp in drive a 6 If necessary enter the name of the TRM Spectrum as trmpic sp and the APV Spectrum as ipvpic sp Browse enables you to search for pathnames and files if
24. Figure 31 Removing the sampling accessory 6 Undo the two locking bolts Figure 32 on the front of the instrument using the hexagonal wrench supplied insert the wrench so that the free end is pointing horizontally away from the instrument and rotate the wrench 180 in the direction indicated by the arrows in the figure Figure 32 Undoing the locking bolts 86 Spectrum 100 Series User s Guide 7 Lift the main cover from the front 8 Ensure the cover is fully open and retained by the stay 9 Perform the necessary maintenance inside the instrument Figure 33 illustrates the primary component parts of a Spectrum 100 Series spectrometer Power Power Supply Unit Supply Unit Laser power Laser power Beamsplitter Beamsplitter behind baffe mm behind baffle ET Figure 33 Spectrum 100 and 100N component parts 10 Close the cover 11 Lock the two locking bolts by inserting the wrench into each bolt in turn and rotating it opposite to the direction indicated by the arrows in Figure 32 12 If required reinstall the sampling accessory by sliding it on the ledge and pushing it into the sample compartment until it engages with the connector 13 If required refit the sample cover by inserting the hinge into the grooves and pushing the cover downwards until the clip engages 14 Reconnect the power cable and switch the power back on 15 If required change the desiccant see page 70 and or purge the instrume
25. MC standard EN 61326 1 EMC standard for electrical equipment for measurement control and laboratory use FCC rules and regulations These products are classified as digital devices used exclusively as industrial commercial or medical test equipment They are exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c 22 Spectrum 100 Series User s Guide Use of Flammable Solvents and Samples WARNING WARNING WARNING The instrument contains a hot source and contact with flammable vapors may cause an explosion When working with flammable solvents or samples particularly during unattended operation with flow cells it is recommended that the instrument optics area should be continuously purged with ary air or nitrogen to maintain a positive pressure and prevent flammable vapor entering the instrument If flammable solvents or samples are spilled on the instrument and there is any possibility that they have entered the interior by coming into contact with cover gaskets for example then the instrument must be switched off immediately and disconnected from the power supply The optics area should then be thoroughly purged with dry air or nitrogen or the main cover should be opened to thoroughly ventilate the optics area before proceeding Flammable solvents or samples should not be stored on or near the instrument Handling of such materials during preparation should b
26. MOLECULAR SPECTROSCOPY SPECTRUM 100 SERIES User s Guide Il PerkinElmer precisely 2 Spectrum 100 Series User s Guide Release History Part Number Publication Date L1050021 October 2005 Any comments about the documentation for this product should be addressed to User Assistance PerkinElmer Ltd Chalfont Road Seer Green Beaconsfield Bucks HP9 2FX United Kingdom Or emailed to info perkinelmer com Notices The information contained in this document is subject to change without notice Except as specifically set forth in its terms and conditions of sale PerkinElmer makes no warranty of any kind with regard to this document including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright 2005 PerkinElmer Inc Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are protected by law PerkinElmer is a registered trademark of PerkinElmer In
27. Pericolo Con il termine WARNING PERICOLO vengono segnalate situazioni che potrebbero provocare incidenti alle persone Troverete informazioni su tali circostanze in un riquadro come questo Warning Waarschuwing Betekent dat wanneer de genoemde aanwijzing niet in acht wordt genomen dit kan leiden tot verwondingen van de gebruiker Warning Aviso Significa que a n o observ ncia da instru o referida poder causar um ferimento ao usu rio Introduction 9 Warning Signs on the Instrument Caution hot surface Caution risk of electric shock Caution laser radiation hazard Caution risk of danger Refer to accompanying documents in all cases where this symbol is used to find out the nature of the potential hazard and any actions which have to be taken 10 Spectrum 100 Series User s Guide Warnings and Safety Information 12 Spectrum 100 Series Users Guide Safety Summary The Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers have been designed to comply with a wide variety of international standards governing the safety of laboratory equipment In routine use the instruments pose virtually no risk to you If you take some simple common sense precautions you can make sure that you maintain the continued safe operation of your instrument DO make sure that the instrument is properly connected to the electrical supply in particular make sure that the ground earth is securely connect
28. REMOVING a aniisi anes 111 Setup Menu 55 Shipping Container OPENIN aaea ee Ea oE 31 Software Instalati iieii inori 38 Software Control BASICS sive eaa iranere nadra anaa aaae 54 Spectrum 100 Series Users Guide 117 Source high operating temperature 87 Location eeeeeen mH 87 Replacing eren 87 114 Terminal Screws eeeeese 88 Spectrum Commands seeeeeeeeee 55 Spectrum 100 and 100N ACCESSOMES ME 28 Capacitors eee 14 Cleaning eco irrada 68 Collecting data from 55 Communications Ports 27 35 Component Parts sesess 86 Connecting the PC uussss 34 Connecting to Electrical Supply 37 Display eren 25 Electrical Supply Inlet 37 GO BUttOri 1 212 25 Guided Tour of sssssesss 24 IEC Pollution classification 15 Instrument Display and Go button 59 Laser Safety Regulations 16 Lifting Points tee 69 MoOVIrig c2 ceo re ec echas 69 Multimedia Introduction 6 Optical System esses 27 PC Connection Port sssss 34 Power Switch ceeeseseeeeeeee 27 P rge FittIngs ecce 77 Purge Ports oii ran darer asiana a anane 77 Purong oaeiio isod a aeeiiaii 76 Requirements
29. Rules and Regulations 21 Filter Holder Installing scifi 2o t eee 95 Filterwheel Filter int prn med iine 94 Filter Holder cesses 94 Foam Spacer ient tren 94 Installing AVI Cell 96 Installing Filters 93 96 LOCAUON rr EE 93 Retaining Screws eese 94 Fuse Changing rre 79 Drawer asasinen aisanana an 79 H HTML help 6 Humidity 13 I Installing an accessory 48 Installing software 38 Installing the instrument 39 Instrument Controls Descriptio Meseia iria 25 59 Display 1e iere e ains 25 GO BUTON surani Tiie 25 Instrument Validation 101 IR Assistant 54 L Labels Front of Instrument 20 Laser Safety eene 19 Sample Area een 20 Warning Signs eeeeeeeeeeennnnn 9 Laser orientation mark eeses 98 Regulations enne 16 Replacing ee 97 114 retaining bracket ssss 98 retaining pin eee 98 securing screws eeeemm 98 Laser and Power Supply cabling eren triti 97 Installation eeeeeeeeeesss 98 Laser Baffle retaining screws seeeeenee 89 Laser Power Supply Locatiori iimi ene anra 97 Replacing eeeenen 97 114 Locking Bolts Undoihg erint ttr 85 M Main Cover Locking Bolt
30. The Spectrum 100 FT IR sample compartment windows are composed of KBr or CsI which although coated can be damaged by high levels of humidity If you spill a liquid in the sample compartment wipe it up quickly When working with water based samples open to the air either purge the sample compartment or leave the cover open For the Spectrum 100 FT IR a relative humidity higher than 75 or 45 f your Spectrum100 FT IR is fitted with CsI optics can damage the windows of the sample compartment If you expect the humidity to exceed 7596 or 4596 if your instrument is fitted with CsI optics continually purge or desiccate the sample compartment The Spectrum 100N FT NIR sample compartment windows are composed of CaF2 which is relatively resistant to humidity An Overview of the Spectrometers 27 Power switch and communications ports The power switch AC power cable connector and communications ports are on the rear of the instrument The power switch is marked I O on off NOTE It can take the Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers up to two hours to equilibrate when switched on after being switched off overnight To save time we suggest that you leave the spectrometer switched on at all times Optical system Usually the main cover of the instrument is closed but to perform most maintenance tasks the cover has to be open When you do this a safety interlock automatically switches off the p
31. They should be cooled in a dry atmosphere For optimum results re activate the packs immediately before re use 76 Spectrum 100 Series User s Guide Purging the Spectrometer Under most circumstances you do not need to purge the optical system However after performing any maintenance tasks that involved opening the main cover you may purge the optical system to remove water vapor and CO that entered while the system was open Purge the sample compartment if you need to make sure that no residual water vapor or CO peaks are visible in the spectrum There are two separate sets of connectors for purge gas lines one for the sample compartment and one for the optical system You can purge with either dry air or nitrogen Both remove water vapor however nitrogen is preferable because it also removes atmospheric carbon dioxide A typical cylinder of dry nitrogen or dry air stores 220 ft at 2200 Ibf in 6 26 m at 1 4 x 104 kPa Make sure that the gas is free of oil water or dirt particles larger than 25 uum 0 001 inch Do not use a flammable gas to purge the instrument The spectrometer contains a hot source and a fire or explosion will result Only use clean dry oil free nitrogen or air to purge the instrument WARNING Never connect the purge tubing directly to a gas cylinder or other high pressure supply always use a pressure regulator and set the pressure to a maximum of 1 Ibf ir 6 9 kPa before you start the flow
32. accessory click Another Accessory You are returned to step 4 9 When any necessary accessory tests and calibrations tests are complete click View Log A new window allows you to see the results in more detail If you want to view the results of the accessories tests later the log is stored at C Program Files PerkinElmer ServiceIR lt Instrument Serial Number gt PerformanceTests xxx PerformanceTests Log where xxx is an incremented number 52 Spectrum 100 Series User s Guide 10 Click Next The Finish page is displayed Spectrum Instrument Install Wizard Finish Instrument Wizard Select Instrument Install Accessories Successfull To view the log click View Log View Log Click Finish to close the Instrument Wizard 11 Click Finish to close the Instrument Install Wizard Using the Spectrometer 54 Spectrum 100 Series User s Guide Basics of Software Control Switching on the Spectrometer NOTE It can take the Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers up to two hours to equilibrate when switched on after being switched off overnight To save time we suggest that you leave the spectrometer switched on at all times 1 Switch on the power to the instrument using the switch above the power cable 2 From the Start menu select Programs the PerkinElmer Applications group the Spectrum sub group and then the Spectrum application After the Spectrum start up splash screen a dialog
33. ates that the product is covered by the Waste Electrical and Electronic Equipment WEEE Directive and is not to be disposed of as unsorted municipal waste Any products marked with this symbol must be collected separately and in accordance with the regulatory guidelines in your area or The objectives of this program are to preserve protect and improve the quality of the environment protect human health and utilize natural resources prudently and rationally Specific treatment of WEEE is indispensable in order to avoid the dispersion of pollutants into the recycled material or waste stream Such treatment is the most effective means of protecting the customer s environment Requirements for waste collection reuse recycling and recovery programs are set by the regulatory authority at your location Contact your local responsible person such as your laboratory manager or authorized representative for information regarding applicable disposal regulations Contact PerkinElmer at the web site listed below for information specific to PerkinElmer products Web address http las perkinelmer com OneSource Environmental directives htm Customer Care USA 1 800 762 4000 inside the USA 1 203 925 4602 outside the USA Customer Care EU 0800 40 858 Brussels 0800 90 66 42 Monza Products from other manufacturers may also form a part of your PerkinElmer system These other manufacturers are directly responsible for the collection and
34. c Spectrum Spectrum 100 and Spectrum 100N are trademarks of PerkinElmer Inc Spectrum 100 Series Users Guide 3 Contents Son LER 3 Introduction 1eeeeeieee rere enn enn nne nennen ann nnne n anu auum ananas 5 About This Mari l eiie tete cose cad cies tin til repe evi eva d ena eere dae 6 Conventions Used in this Manual eese nennen 7 Notes Cautions and Warnings eeeeseeeeeeenennmennne 7 Warning Signs on the Instrument eeseeseeeenem enne 9 Warnings and Safety Information eeeeenenen nnns 11 Safety S mtmary 5i eei ecce d estie eorr en reet T eee LA POOR Te TEES ERU E E EUR gd 12 General Safety srs onise tete eI ecd ee IURE YE EEV ER DEXTRO ERE NRE 13 Location and ventilation eeeseeeeeee eene 14 Electrical Safety iicet te eter rear rer rece eerie esu ve Ue 14 Laser Safety Regulations seeeesssseseseseesenenmenennnnen nennen 16 Explanation of the laser radiation hazard and its classification 17 Easer Apertufes 12 exerit deir eR het tc na eoe Leap oe DR MER REN EN ARS NEA EYE 18 Radiation Emitted by the Spectrum 100N FT NIR sesee 18 E Jc EE 19 Spectrum 100 FT IR and Spectrum 100N FT NIR laser safety labels 19 Product identification label eeeeeeeeeeee nennen 20 EME ComplianCe ts 2 rei Eee dre erede e um Ee x ee mea d
35. connect the two wires to the terminals and secure by tightening the two terminal screws Top of connector block Terminal screw Terminal screw Source Source housing Figure 35 Terminal screws 6 Close and secure the Main Cover steps 10 to 15 on page 86 7 Plug in and switch on the instrument 8 Allow the source temperature to stabilize for approximately 2 hours before using the instrument Advanced Maintenance 89 Replacing the Beamsplitter Switch off the mains power supply to the spectrometer wait 60 seconds and disconnect the power cable before you open the cover of the spectrometer This makes sure that you are safe from electrical Shock and laser radiation WARNING 1 Open the main cover of the instrument as detailed on page 84 and identify the Beamsplitter which is behind the laser baffle Figure 36 Beamsplitter Beamsplitter behind baffle behind baffle Figure 36 Beamsplitter location 2 Unscrew the laser baffle retaining screws and remove the laser baffle as shown in Figure 37 Beamsplitter retaining screw black Laser baffle retaining screw silver Laser baffle retaining screw silver Laser baffle Figure 37 Laser Baffle 90 Spectrum 100 Series User s Guide Remove the beamsplitter retaining screw Noting its installed position pull out the beamsplitter Install the new beamsplitter and secure with the retaining screw Refit the lase
36. containing data required to perform shipping checks on the accessory Use the browser to find the accessory files and press Copy Accessory Data If you cannot locate the files press Cancel Lookin sss s elel 9 AcyIdent txt Browse for file AcyIdent txt Cancel Copy Accessory Data Unpacking and Installation 51 7 Browse to the configuration file select it then click Copy Accessory Data The software performs a series of appropriate accessory performance tests Spectrum Instrument Install Wizard j Install Accessories Instrument Wizard i e Select Instrument Testing your accessory MM e ACC Accessory Detect Universal ATR DiamondiZnSe 1 o In ssories gt Installing Accessories Accessory Disk Passed Contamination Passed Finished Throughput Passed Noise test not required Mechanical test not required Wavelength Calibration test not required Completed To install another accessory click Another Accessory To view the log click View Log View Log If you do not wish to install anymore accessories click Next Back Finish You may be prompted to remove the accessory briefly to clear the beam path whilst a background spectrum is collected then replace it 8 Once installed an accessory is recognized by the Spectrum software automatically whenever it is fitted to the instrument You may decide to install and test all the accessories shipped To install another
37. control the instrument and manipulate the spectra that you collect An Overview of the Spectrometers 25 Top Panel Controls Figure 5 Top Panel Controls The Display on the lower right of the top of your instrument has two purposes 1 To display messages generated by the instrument s firmware such as those that monitor initialization and diagnostics when the instrument is switched on 2 To display prompts and other messages generated by the Spectrum software The instrument s Go button is used in concert with the displayed prompts and other messages generated by the Spectrum software It allows you to Start and Halt data collection while you are away from your computer 26 Spectrum 100 Series User s Guide Sample compartment The sample compartment Figure 6 is located at the front of the instrument You open the sample compartment by lifting the cover using the recess at the front Figure 6 Sample compartment e The infrared beam enters the compartment through an aperture on the left After passing through the sample it enters the detector area through an aperture on the right side of the sample compartment e Standard accessories are mounted on a baseplate that plugs into the connector on the rear wall of the sample compartment There are other accessories that are mounted in the sample slide or on the sample shuttle e The standard baseplate has a sample holder located by a knurled screw CAUTION CAUTION
38. ctrum is collected and a signal light green 9 amber _ or red is placed in the QC column to indicate its status To examine Quality Check results in more detail select the appropriate scan then click the button Working with the Instrument Display and Go button Figure 12 Instrument Display and Go button The Display on the lower right of the top of your instrument is used to display prompts and other messages generated by the Spectrum software The messages and prompts provided by the Scan Progress dialog and the actions of the Scan Halt button are mirrored by similar messages and prompts on the Instrument Display and the actions of the instrument s Go button When used in conjunction with the Multiple Sample table in the Spectrum software you can complete a series of sample scans using the Display and Go button 60 Spectrum 100 Series Users Guide Using the Spectrum on screen help system Use the Spectrum HTML Help system to find further information about using Spectrum software to control set up and adjust your instrument To open the Help file select Contents and Index from the Help menu This menu also includes links to on screen tutorials Learning Spectrum a simplified interface IR Assistant and information about the software About To open the Help file at the most relevant topic press the F1 key or click the Help button sometimes the button in the window where you are working For example
39. dag 21 EC dif ectiVe 2s iiie eo ei tei Ene E ERE EFE KNEE DR EEEER eX YR NE RE 21 FCC rules and regulations eseeeeeeeennmmm een 21 Use of Flammable Solvents and Samples eene 22 An Overview of the Spectrometers e eeeeneneennre 23 A Guided Tour of the Spectrum 100 Series cccccccsseceeseeeessseesseeeeseeees 24 Top PatielContfols rn eese eere o onde thea eaa aasa aiiai 25 Sample compartment eeeeeseeee eene nennen nennen nnn nnn nnn nnn nn 26 Power switch and communications ports eeeneenne 27 Optical system ace eL en tibet 27 Accessories for the Spectrum instrument eeeseeeeeennnenennnnne 28 Unpacking and Installation e ener 29 Requiremeritszx 5e et eei i ie fa cua o acide ore Te te rye e dr demas 30 Electrical requirements esseeeeeeeeeeee eene 30 ERVIFOTITIIeDLU eie ein rnc rtr eara cra eode eer eed Ere eara 30 Unpacking the Spectrometer eeeeeeeeenen mmm 31 Opening the shipping container eeeeseeeeennn mm 31 The Desiccant Indicator eeeeeeeeee sienne nnne nennen nennen nnn 33 Connecting up the Spectrometer seseeeeeen nene nnns 34 Connecting tothe PC siscitiier dees eee anni de esi eer Pao pue co Er eie RR de 34 Other Conriectors recor eea aun iare era evene en
40. e can be hazardous depending upon the power of the beam the length of time that the eye is exposed to the beam and the optical efficiency of the exposed eye Direct viewing of a laser beam along its axis is termed ntrabeam viewing Protection of the eye during accidental momentary intrabeam viewing of a Class II Class 2 laser beam is normally given by the eye s aversion response including the blink reflex which limits exposure of the eye to less than 0 25 seconds CDRH regulations state that Class IIa levels of laser radiation are not considered to be hazardous if directly viewed for any period of time less than or equal to 1000 seconds Class I Class 1 levels of laser radiation are not considered to be hazardous The CDRH BS EN 60825 1 and IEC 60825 1 laser radiation classification limits are described below CDRH Class limits Classification For 633 nm Laser Radiation Class II gt 3 9 uW to 1000 uW Class IIa gt 0 39 uW to 3 9 pW Class I lt 0 39 uW BS EN 60825 1 and IEC 60825 1 Class limits Classification For 633 nm Laser Radiation Class 2 gt 6 8 uW to 1000 uW Class 1 lt 6 8 uW 18 Spectrum 100 Series Users Guide Laser Apertures The accessible Class I Class 1 laser radiation emerges from the aperture in the left hand side of the sample compartment the radiation crosses the sample compartment and enters the detector aperture Class I Class 1 laser radiation exits the external beam port when the port cover is
41. e performed in a safe area away from the instrument such as a fume cabinet An Overview of the Spectrometers 24 Spectrum 100 Series User s Guide A Guided Tour of the Spectrum 100 Series Figure 4 The Spectrum 100 Series spectrometers The PerkinElmer Spectrum 100 Series spectrometers Figure 4 are bench top instruments that provides all the following in one self contained unit A large purgeable sample compartment The instruments can operate in ratio single beam or interferogram mode For the Spectrum 100 FT IR an optical system that gives you data collection over a total range of 7800 to 370 cm 220 cm with CsI beamsplitter with a best resolution of 0 5 cm For the Spectrum 100 FT IR a mid infrared detector either DTGS deuterated triglycine sulphate or LiTaO lithium tantalate as standard and the option of using MCT Mercury Cadmium Telluride or PAS a photoacoustic detector For the Spectrum 100N FT NIR an optical system that gives you data collection over a total range of 14000 to 2100 cm with a best resolution of 0 5 cm For the Spectrum 100N FT NIR a near infrared detector NIR DTGS deuterated triglycine sulphate as standard An electronics system based on the Motorola DSP56303 Digital Signal Processor and the Motorola 68340 Integrated Processor The instrument is connected to a PC either point to point or over a network which utilizes Spectrum software This software enables you to
42. e fades leaving the instrument identity message PerkinElmer Spectrum 100 Series When connected to a PC the Instrument Display adds Ready e When collecting data the Instrument Display provides prompts generated by the PerkinElmer application software 114 Spectrum 100 Series User s Guide Initialization Errors If your instrument generates one of the following error messages switch off the instrument then switch the instrument on again Consult your PerkinElmer service representative if the problem persists Error message Action Program Integrity Failed The instrument s firmware may be corrupt so must be reinstalled Instrument Configuration Failed Suggests that one or more internal devices is failing If the problem persists note any associated error messages and consult your PerkinElmer service representative Scan Failed If the problem persists note any associated error messages and consult your PerkinElmer service representative Laser Failed Refer to Replacing the Laser and Power Supply on Replace Laser page 97 Source Failed Replace Source Refer to Replacing the Source on page 87 Calibration Failed Too little energy may be reaching the detector Make sure the sample compartment is clear Spectrum 100 Series Users Guide 115 Appendix 3 WEEE Instructions for PerkinE mer Products A label with a crossed out wheeled bin symbol and a rectangular bar indic
43. e instrument optics may be fogged Do not switch the instrument either ON or OFF until all sectors are blue Sector Action Required All sectors blue None The humidity levels in the instrument are below approximately 10 Relative Humidity Sector 10 pink Change the desiccant If the instrument has been switched OFF for an extended period do not switch ON until you have changed the desiccant and all sectors are blue Sector 10 amp 15 You should have changed the desiccant already pink Do not switch the instrument ON or OFF until you have changed the desiccant and all sectors are blue Routine Maintenance 71 Changing the Desiccant NOTE We recommend that you change the desiccant in the spectrometer before using it for the first time Expect to change the desiccant in the spectrometer every six months Old used desiccant releases moisture CAUTION In regions experiencing high humidity levels we recommend you change the desiccant more often At intervals set in the Maintenance section of the instrument control software the following message will be displayed to warn you that the desiccant in your instrument needs changing Figure 18 Scan and Instrument Setup f The desiccant in your instrument may be or is due for replacement If your instrument has a desiccant indicator check the desiccant condition If your instrument does not have an indicator change your desiccant Figure 18 Des
44. e term instrument refers to either the Spectrum 100 FT IR or Spectrum 100N FT NIR spectrometer and any sampling accessory fitted Notes Cautions and Warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates additional significant information that is provided with some procedures We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment Details about these circumstances are in a box like this one Co Caution Achtung Bedeutet daB die genannte Anleitung genau befolgt werden muB um einen Ger teschaden zu vermeiden amp Caution Bem rk Dette betyder at den n vnte vejledning skal overholdes n je for at undg en beskadigelse af apparatet e Caution Advertencia Utilizamos el t rmino CAUTION ADVERTENCIA para advertir sobre situaciones que pueden provocar aver as graves en este equipo o en otros En recuadros ste se proporciona informacion sobre este tipo de circunstancias CF Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves d t riorations de l instrument ou d autre mat riel Les d tails sur ces circonstances figurent dans un encadr semblable celui ci CD Caution Attenzione Con il termine CAUTION ATTENZIONE vengono segnalate si
45. ect spectra The detector is mounted in a dewar that can be filled with liquid nitrogen The detector is not enabled until it has been sufficiently cooled WARNING WARNING WARNING The extremely low temperature of liquid nitrogen can burn skin and eyes Avoid exposure by wearing protective gloves and safety goggles whenever you work with it As liquid nitrogen cools the detector it evaporates so rapidly that the resulting pressures can send a funnel or detector cap suddenly and forcefully shooting upward from the top of the dewar Be sure to wait the specified time when filling the funnel and before replacing the plug This enables the bubbling nitrogen to settle down and the pressure to dissipate In addition to wearing safety goggles at all times stand back from the instrument after each time you fill the funnel 1 Remove the MCT coolant cover Figure 28 MCT coolant cover Figure 28 Location of MCT Coolant Cover 2 Remove the plug from the dewar 3 Ensure that the funnel guide which helps prevent overspill entering the instrument body rather than the dewar is in place 82 Spectrum 100 Series User s Guide 4 Make sure the supplied plastic funnel is dry Stand where you can see the inside of the funnel as you pour the nitrogen in Pour slowly so that neither the funnel nor the dewar CAUTION overflows Take care not to overfill the funnel and splash liquid nitrogen onto the instrument covers
46. ed DO disconnect the electrical power cable before opening the main cover of the instrument DO keep the instrument dry Avoid spilling liquid into the instrument Clean all external spills immediately If anything that is spilled enters the main body of the instrument switch off the power and contact a PerkinElmer Service Engineer DO NOT stare into the internal laser beam under the instrument cover The instrument contains a low power visible red laser momentary exposure to the beam is not dangerous but deliberate direct viewing of the beam along its axis could damage your eye DO NOT use a flammable gas to purge the instrument The instrument contains a hot source and a fire or explosion will result Only use clean dry oil free nitrogen or air to purge the instrument DO read the more detailed information on warnings and safety in the following pages to ensure the safe operation of the instrument Warnings and Safety Information 13 General Safety The Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers have been designed and tested in accordance with PerkinElmer specifications and in accordance with the safety requirements of the International Electrotechnical Commission IEC The instruments conform to IEC publication 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use as it applies to IEC Class 1 earthed appliances and therefore meets the requirements of EC lo
47. eference spectra ASTMO Reference Spectra d Passed Collection rence Spectra Completed gt Collecting ASTM Reference Spectra Finished To view the log click View Log View Log lt Back Finish 14 When the ASTMO Reference Spectra collection is complete click View Log A new window allows you to see the results in more detail If you want to view the results later the log is stored at C Program Files PerkinElmer ServiceIR lt Instrument Serial Number gt PerformanceTests xxx PerformanceTests log where xxx is an incremented number 15 Click Next The Finish page is displayed Spectrum Instrument Install Wizard Instrument Wizard Unpacking and Installation 47 Test Configuration Test Performance Collect ASTM Reference Spectra Select Instrument eference Spectra ished To view the log click View Log Successfull Successfull Successfull View Log Click Finish to close the Instrument Wizard This page of Instrument install wizard offers a summary of the Configuration and Performance Tests You can also click View Log to see the results of the configuration and performance tests in more detail 16 Click Finish to complete the installation and close the Instrument Wizard 48 Spectrum 100 Series User s Guide Using the Instrument Install Wizard to install an Accessory The Instrument Install Wizard tests your accessory against for example any shipping damage a
48. efore you open the cover of the spectrometer This makes sure that you are safe from electrical shock and laser radiation WARNING 1 Remove the filterwheel assembly as described in Installing Filters in the Filterwheel on page 93 2 Place the cell in the next available filter position orientated as shown in Figure 46 and Figure 47 and place the spacer on top of it Filter holder Figure 46 AVI Cell orientation Figure 47 AVI Cell installation 3 Secure the cell and refit the filterwheel Refer to page 95 steps 8 to 11 4 Update your Spectrum configuration so that the software is aware of the new filter Refer to Filterwheel in the on screen help system Advanced Maintenance 97 Replacing the Laser and Power Supply Switch off the main power supply to the spectrometer wait 60 seconds and disconnect the power cable before you open the cover of the spectrometer This makes sure that you are safe from electrical shock and laser radiation WARNING Do not attempt to separate the Laser from the power supply The Laser retains an electrical charge even when the main power is switched off WARNING 1 Open the Main Cover of the instrument as detailed on page 84 and identify the Laser and Power Supply Figure 48 Power supply Power supply Figure 48 Laser and power supply 2 Slacken the two screws securing the laser power supply 3 Disconnect the thin black and red cables marked
49. er rhe eer eB rara Eta 35 Connecting the Spectrometer to the electrical supply 37 Installing the Spectrum Software s sssssessssrsrrnrnsurernnnnnnnnenennnnnnnnnenennnan 38 Minimum PC requirements eeeseeeeeeenenn enne 38 Installing Spectrum software eeseeseseeseseenemeeennne 38 The Instrument Install Wizard eesseeeeeeeeeee nennen 39 Using the Instrument Install Wizard to install an Accessory 48 Using the Spectrometer 1 eeeeeeeseee ener 53 Basics of Software Control essen enne nnne nnn 54 Switching on the Spectrometer sssssssssssrrrrnrrsesrurnnnnrrnenennnnnnnnnenne 54 Controlling the INStrUMeNt c eeeeeee cece eect terete eeeeeeaaaaeeeeeeeeeeeeaee 55 Working with the Instrument Display and Go button 59 Using the Spectrum on screen help system eeneeee 60 Atmospheric CO H O Suppression eeeeeeeeen enne 61 What is atmospheric suppression eeeeeeeeennnnnm 61 What does atmospheric suppression do eeeseeeeeees 61 AVI COITOCEIOD 5 ccd oso ee eee epus sh ea ER e EE e o EORR EESYRETX EISE TA 63 What is AVI correction sseeeseseeeee nennen nennen nnne 63 What does AVI correction do eesseeeeeneeenn mnm 63 took Ahead ioter Hn vanes erp der Dee dace
50. es very low friction point bearings and a frictionless electromagnetic drive to ensure long life 28 Spectrum 100 Series User s Guide Accessories for the Spectrum instrument There is a range of specialized sampling accessories available for the Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers including Accessory Spectrum 100 FT IR Spectrum 100N FT NIR Sample Shuttle Yes Yes HATR Yes n a Universal ATR Yes n a Diffuse Reflectance Yes n a NIRA n a Yes NIR Tablet Autosampler n a Yes NIR Fiber Optic Probe n a Yes Liquid Sipper Yes Yes These accessories are easily installed see Changing the Sampling Accessory on page 110 and will be automatically recognized and controlled by the software Further information about the use of these accessories can be found on the Spectrum 100 100N Manuals CD and on the Spectrum Multimedia CD Figure 7 Spectrum 100 with Universal ATR accessory fitted Unpacking and Installation 30 Spectrum 100 Series User s Guide Requirements NOTE Read the warnings and safety information at the start of this manual before you install the instrument They contain important information Electrical requirements The Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers can operate on electricity supplies of 50 or 60 Hz and in the 100 to 230 V range without any adjustment The nominal power consumption of the instrument is 120 VA The line supply must be
51. f the HeNe laser radiation with a maximum power level of less than 0 39 uW Class I Class 1 may be accessed in the sample compartment or a sampling accessory The laser is automatically shut down when the main cover of the instrument is raised The instrument complies with the following laser safety regulations 1 21 CFR Chapter 1 Subchapter J Radiological Health Part 1040 10 administered by the Center for Devices and Radiological Health U S Department of Health and Human Services 2 British Standard BS EN 60825 1 1994 Radiation safety of laser products equipment classification requirements and user s guide BS EN 60825 1 implements CENELEC European Normalization document EN 60825 1 CENELEC is the European Committee for Electrotechnical Standardization 3 IEC Publication 60825 1 1993 and Amendment 1 Safety of laser products Part 1 Equipment classification requirements and user s guide IEC is the International Electrotechnical Commission The IEC have renumbered their standards IEC Publication 60825 1 was originally IEC Publication 825 1 Do not attempt to override or modify the interlock system WARNING Warnings and Safety Information 17 Explanation of the laser radiation hazard and its classification Indirect observation of the laser beam radiation in the optical path is not hazardous Directly viewing the laser beam along its axis allowing the laser beam radiation to pass into the ey
52. fore starting the calibration you make sure that the instrument is optimally aligned Refer to the A igning the Instrument topic in the Spectrum on screen help NOTE The NIR IPV Certificate Printing Utility validates the basic instrument and cannot be used with an accessory For information on how to validate accessories and how to perform T and noise tests see the Va idation section of the Spectrum on screen help Collecting the Reference Spectra NOTE For information on collecting spectra see the Spectrum on screen help 1 Set up the instrument with the settings shown below Refer to Controlling the Instrument on page 55 2 Collect a background spectrum of the empty sample compartment using 16 scans 3 Put the TRM in the sample slide in the sample compartment 4 Collect a sample spectrum of the TRM using 16 scans 5 Save the spectrum on a disk as trmpic sp You may use another name for the spectrum 6 Replace the TRM in its plastic box and store it in a safe place 7 Move the internal polystyrene APV filter into the beam path 8 Select Instrument from the Setup menu in Spectrum 9 Select the Beam tab and then click on the Filterwheel 10 Select polystyrene and click OK 11 Collect a ratio spectrum of the polystyrene using 16 scans 12 Save the spectrum as ipvpic sp on the same disk You may use another name for the spectrum 102 Spectrum 100 Series User s Guide Printing the
53. gure 16 Two people are needed to lift it because its basic weight is approximately 34 kg Figure 16 Spectrum 100 Series lifting points Condensation Be aware that condensation caused by moving your spectrometer from a cooler environment to a warmer one can damage the windows of the sample compartment To prevent this damage from occurring Make sure that the windows are protected by placing fresh bags of desiccant in the sample compartment e Leave the spectrometer to reach the temperature of the surroundings before removing the desiccant 70 Spectrum 100 Series User s Guide The Desiccant Indicator in detail The optical system of the spectrometer is purged at the factory This protects the KBr Spectrum 100 or CaF Spectrum 100N beamsplitters and the sample compartment windows from being damaged by humidity Replaceable packs of desiccant maintain the purge The top panel of the instrument includes a Desiccant Indicator whose sectors change sequentially from blue to pink as the desiccant becomes exhausted Change the desiccant packs in the instrument when the sector marked 10 becomes pink but while the sectors marked 15 and 20 are still blue These numbers correspond to the approximate Relative Humidity in the optical compartment Figure 17 The Desiccant Indicator When desiccant packs should be changed If all three sectors of the Desiccant Indicator are pink then you must change the desiccant CAUTION Th
54. he Instrument menu where you can e Enter the details to be saved with the spectra on the Multiple Sample tab NOTE Click the adi icon to toggle between the Multiple Sample tab and the Sample tab interface which may be familiar from earlier versions of the Spectrum software The Multiple Sample table is shown below with rows for four spectra For the Name fields Data Collection has been set up to prefix each sample name with the word Sample and to Auto increment 001 002 003 and so on To add a Description for each row select the appropriate cell To append a comment to one or more rows click the 3 icon 7 Scan and Instrument Setup File Setup Help Q 28 0 d X Multiple Sample Scan Instrument Beam Accessory Number of Samples 4 IV Preview Scan Name Description T Sample 001 Description 1 2 Sample 002 Description 2 3 Sample 003 ipti 4 Sample 004 EREEERIR IS LER NOTE When you fit certain accessories such as the NIR Tablet Autosampler the format of the Multiple Sample table is amended For further details refer to the Spectrum HTML Help system 56 Spectrum 100 Series User s Guide e Select the Range Scan type Units and Scan number number of scans on the Scan tab 7 Scan and Instrument Setup File Setup Help SGYIHOGRE Multiple Sample Scan Instrument Beam Accessory Start 4000 00 cm End 450 00 cm r Options Sca
55. he orientation is not correct write Top on another sheet of paper use a different orientation and repeat step 2 Continue doing this until you have found the correct orientation Printing the certificate 1 Put the certificate in the paper tray in the correct orientation 2 Click Print The certificate is printed see the example on the next page wall Instrument Performance Validation 105 pP i PerkinElmer precisely Certificate of Performance Validation LL fi Instrument Model Number Instrument Serial Number NPL Certified Reference Material Serial No JR95 Nominal nm 1763 9 2187 4 Nominal cm 1 5669 3 4571 6 Date User Identification Signed NPL National Physics Laboratory Teddington England APV Automatic Precision Validator Printed in the UK Copyright 1999 PerkinEImer Inc PerkinElmer is a registered trademark of PerkinElmer Inc Traceable Reference Material TRM Serial No PA00047 Measured nm 1763 9 0 4 2187 4 0 4 Measured cm 1 5669 3 1 5 4571 7 1 0 18 Jan 2005 NIR ADMIN Spectrum 100N 50177 Reference Material Used in Routine Validation Measured nm 1763 9 2187 4 Measured cm 1 5669 3 4571 7 i All analytical instruments and systems manufactured by PerkinElmer Ltd are certified under the quality requirements of ISO9001 Certificate number FM11397 L118 8353 i
56. ibrated against this CRM and a Certificate of Calibration printed at the factory is included with it This certificate provides a record of calibration and traceability for the TRM to the NPL CRM The polystyrene in the APV is calibrated by the procedure described in the following pages A certificate of Performance Validation will be printed which details the calibration of the polystyrene in the APV against the TRM The certification procedure also validates the internal APV used by Spectrum 100N for Ready for Work checks For more information see the Spectrum on screen help which can be accessed by selecting Contents and Index from the Help menu in Spectrum Limitations of traceable wavenumber calibration Certified values for the peak positions used have quoted uncertainties of 1 5 cm for the peak at 5669 3 cm and 1 0 cm for the peak at 4571 6 cm We suggest that the uncertainty associated with routine measurement of the two recommended peaks in the spectrum of a traceable polystyrene sample is realistically set at these limits This level of precision is satisfactory for general analytical applications involving solid and liquid samples Instrument Performance Validation 101 Validating Instrument Calibration In order to validate instrument calibration tests must be performed under the same measurement conditions as those used to calibrate the Traceable Reference Material TRM at the factory We also recommend that be
57. icate click Save The Specify IPV Result File dialog is displayed 11 Choose the Drive Folder and File Name 12 Click OK The file is saved as an ipv file The Specify IPV Result File dialog closes and the certificate information is redisplayed To print the certificate see page 104 104 Spectrum 100 Series User s Guide Possible Reasons for Validation Failure If the peaks were not found or lie outside the tolerance the failure may be because e the wrong spectra were used e the wrong reference material was used to collect one of the spectra e theinstrument was not aligned correctly e the noise level is too high e the beam was obstructed during validation for example by an accessory in the beampath This effect is most often seen at high wavenumbers e theinstrument is not set up correctly The instrument setup is not correct The incorrect J stop aperture was used most often seen at high wavenumbers The resolution used for collecting the two spectra was not the same If you need more help contact your nearest PerkinElmer agent or office Printing the Certificate Testing for paper orientation Before you print the certificate you must find the correct orientation for the certificate 1 Write Top on a sheet of plain paper and put it into the paper tray of your printer with the writing visible and towards you this will depend on your printer type 2 Click Draft The test sheet is printed 3 If t
58. iccant Replacement Warning Dialog When the message appears you should check the Desiccant Indicator The message will continue to appear until you tell the software that the desiccant has been changed NOTE The following operations can only be performed by a user who has Advanced level instrument access as set on the Instrument tab of the Options dialog 72 Spectrum 100 Series User s Guide Resetting the desiccant change interval The message displayed to warn you that the desiccant in your instrument needs changing Figure 18 is displayed until the Desiccant options are reset using the Maintenance tool in the Adjustment Toolbox 1 Display the Setup menu and choose Instrument The Scan and Instrument Setup dialog is displayed Scan and Instrument Setup Q 258 C X Sample Scan Instrument Beam Accessory Details Name Description Comments M Preview Scan 2 Click The Adjustments Toolbox is displayed Adjustments Toolbox Maintenance Change the service interval or desiccant on your instrument Wal Set the wavelength calibration for your instrument Align Align your instrument Centre Burst Find the centreburst position for your instrument AVI Calib Perform and AVI calibration for your instrument Instrument Display Change the brightness and contrast settings on your instrument display Routine Maintenance 73 3 Click Maintenance The Maintenance dialog
59. inearly to 50 relative humidity at 40 C Mains supply fluctuations not exceeding 10 of the nominal voltage 14 Spectrum 100 Series Users Guide Location and ventilation To allow for adequate cooling the instrument should not be sited near to room heating equipment for example central heating radiators During operation there should be a minimum gap of e 15 cm 6 inches between any surface and the cooling louvers at the rear of the instrument e 7cm 3 inches between the instrument and adjacent equipment e 45cm 18 inches between any surface and the top surface of the closed sample area lid to allow for the lid to be opened fully Make sure that the switch at the electrical supply inlet on the rear of the instrument is not obstructed WARNING Electrical safety Connect the instrument to a power supply line that includes a switch or other means of disconnection from the electricity supply e Only plug the instrument into an electricity supply socket that is provided with a protective earth connection e When fuses need replacing use only those with the required current rating and of the specified type Do not use makeshift fuses and do not short circuit fuse holders e When the instrument is connected to its electricity supply terminals may be live and the removal of covers other than those which can be removed by hand is likely to expose live parts Capacitors inside the instrument may s
60. ion is complete and displays the Instrument Type recognized by the Spectrum software 8 When this page is Completed check that the information displayed is as expected Likely causes of any discrepancies include out of date firmware or configuration data Ask your PerkinElmer Service representative for advice Unpacking and Installation 43 9 Remove the configuration CD and click Next The wizard now offers the opportunity to Test the performance of your instrument Spectrum Instrument Install Wizard Test the performance of your instrument Instrument Wizard sct Instrument This wizard helps you install a new instrument Performance A series of tests may be run on your instrument These tests will confirm gt that the instrument is operating within the parameters specified by Test Performance PerkinElmer A report is generated summarizing the results of the ng Performance performance tests M Reference Spectra Finished To continue with the tests click Next To skip the tests click Skip Skip 10 If you want to continue with the performance tests click Next Otherwise click Skip e If your instrument is an FT IR spectrometer and you elect to skip performance tests the wizard continues with the collection of ASTM Reference Spectra e If your instrument is an FT NIR spectrometer and you elect to skip performance tests the Finish page is displayed where you can view the Log for this installation
61. is displayed Maintenance Desiccant Last changed 03 03 2005 11 37 37 Change interval E days Cancel Changed Service Last serviced 03 03 2005 11 37 44 Service interval 360 days Serviced 4 The date of the last desiccant change is reported in the Desiccant panel Select the Changed checkbox and if required reset the Change interval The message displayed to warn you that the desiccant in your instrument needs changing will reappear after the specified number of days 5 If appropriate update the Service panel 6 Click OK Renewing the disposable desiccant Old used desiccant releases moisture and can cause catastrophic failure of KBr optics CAUTION Do not use damaged packs of desiccant Make sure that the packs you use have not been left in contact with the air You can purchase disposable desiccant kits from PerkinElmer Part Number NO17 1159 A kit contains two packs of desiccant and three kits are required 1 Inspect the humidity indicator card in the plastic bags in which the spare desiccant packs are packed If the card indicates humidity in the bag discard the desiccant pack 2 Remove the sample area cover if fitted by opening the cover pressing the clip and pulling the cover vertically to remove 74 Spectrum 100 Series User s Guide Desiccant Indicator Sample cover release clip Figure 19 Removing the sample area cover 3 Undo the two captive screw
62. ling the Spectrum Software Before setting up the instrument set up your PC and install the Spectrum software Minimum PC requirements If you are supplying your own computer make sure that it meets the minimum requirements for hardware and software set out in the PC Requirements section of the Spectrum Administrator s Guide L1050020 Installing Spectrum software Details of the installation program are given in the Spectrum Administrators Guide L1050020 Unpacking and Installation 39 The Instrument Install Wizard NOTE Install your instrument with the standard sample slide fitted rather than any additional accessory such as a Universal ATR for the Spectrum 100 FT IR or the NIR Tablet Autosampler for the Spectrum 100N FT NIR Accessories are shipped in separate boxes and fitted later Make sure the sample area is clear and there is nothing in the sample slide 1 To install your instrument Log In to the Spectrum software as an administrator and elect to work Offline 2 From the Administration menu select Instrument and Accessory Configuration then select Add Instrument from its sub menu The Instrument Install Wizard starts with advice on how to connect your instrument to your PC or network Spectrum Instrument Install Wizard Welcome to the Instrument Wizard Instrument Wizard Select Instrument This wizard helps you install a new instrument gt New Instrument Your instrument must be swi
63. may fail with age and will need to be changed NOTE If fuses fail repeatedly there is an electrical fault disconnect the power supply and contact your PerkinElmer Service Department There is one spare fuse in the fuse drawer If you need more order 2A 250V Time Lag fuses 0497 0839 from PerkinElmer You must only replace the fuse with one of this type and rating Do not use makeshift fuses and do not short circuit fuse holders Before you begin this procedure switch off the power to the spectrometer wait for 60 seconds and disconnect the power cable This will protect you from electrical shock WARNING The external fuse drawer is located at the rear of the instrument between the power switch and the power socket Figure 26 Fuse Drawer Figure 26 Fuse drawer closed 1 Lever out the fuse drawer so that it swings down over the power socket The fuse is in the right hand slot 2 Remove the old fuse and discard it 80 Spectrum 100 Series User s Guide 3 Fit the new fuse into the slot Figure 27 Spare Fuse Figure 27 Fuse fitted drawer open 4 Close the drawer The new fuse is now installed 5 Reconnect the power cable and switch on the instrument Routine Maintenance 81 Cooling the MCT Detector Spectrum 100 Optionally the Spectrum 100 may be fitted with an MCT mercury cadmium telluride detector When it is fitted in the instrument it must be cooled to 77 K before you coll
64. n type Sample v Scan minimized Units XT r Duration Scan number 4 C Scan time 1 minutes e Select the instrument settings required on the Instrument tab The Advanced options of CO2 H20 suppression AVI correction Look Ahead and Quality Checks are discussed between pages 61 and 65 Scan and Instrument Setup Q 38 0 d s Multiple Sample Scan Instrument Beam Accessory r Settings Resolution Advanced Datainteva fil V auto co2H20 Apodization Strong AM Phase correction Magnitude gt Look Ahead Scan speed 020 z Quality Checks Using the Spectrometer 57 Set up items on the beampath on the Beam tab The Beam tab utilizes an interactive graphic Hover over its icons to identify elements in the instrument beam path Where appropriate right click for further detail about or an options dialog for the element Scan and Instrument Setup Q 18 0 d d Multiple Sample Scan Instrument Set up accessory specific conditions or call up specific pre set configurations on the Accessory tab Scan and Instrument Setup Multiple Sample Scan Instrument Beam Accessory r Configuration User defined Details Sample Slide Sampling Specular Reflectance MIR KBr Disc 3M Card 58 Spectrum 100 Series User s Guide 2 When you are satisfied with the entries on the Scan and Instrument Setup dial
65. nd makes sure that any data necessary for its operation is present on your system NOTE If you want to test an Accessory after it has been installed there is no need to re run these accessory installation tests Validate your instrument by running System Suitability checks instead 1 To install an instrument accessory Login to the Spectrum software as an Administrator 2 From the Administration menu select Instrument and Accessory Configuration then select Add Accessories from its sub menu The Instrument Install Wizard starts Spectrum Instrument Install Wizard 4 E Welcome to the Instrument and Accessory Wizard Instrument Wizard This wizard helps you to configure and test a new accessory First you must select the instrument you wish to use for your accessory installation Select Instrument gt Existing Instrument Select Instrument Spectrum 100N v Unpacking and Installation 49 3 Select the instrument on which the accessory will be installed then click Next The Install Accessories page is displayed Spectrum Instrument Install Wizard Install Accessories Instrument Wizard Some accessories are shipped with an Accessory Disk containing data generated during the manufacture of the accessory This data must be transferred to the system in order for the accessory to function correctly In addition some accessories require some one off performance tests to ensure
66. nt Instrument Configuration Disk A configuration file is required For your instrument in order For it to Function correctly Your instrument was shipped with this configuration data stored on a floppy disk or on a CD Please insert this disk and locate the configuration File using the browser below Lookin ARETE Browse for file C90001 cfg Cancel If a suitable configuration file has been has been installed on your PC on a previous occasion this dialog is amended to enable you either to Use Existing Configuration or to Overwrite Configuration 42 Spectrum 100 Series User s Guide 7 Browse to the configuration file then click Copy Configuration The Test Configuration page is displayed Spectrum Instrument Install Wizard 4 Ej Test Configuration Instrument Wizard Select Instrument Testing the connection and configuration of your instrument Instrument Details Connection Connected gt A Testing Configuration Serial Number c74131 Performance Firmware Version 00 02 6443 ASTM Reference Spectra Finished Instrument Configuration Configuration Complete Instrument Type Spectrum 100N Completed lt Back Cancel This test automatically checks whether your PC and instrument can communicate with each other Assuming this Connection is working the page then displays the Serial Number and Firmware Version reported by your instrument confirms that the installation of the Instrument Configurat
67. nt see page 76 If the cover is left open for more than 1 hour the desiccant should be replaced Advanced Maintenance 87 Replacing the Source WARNING WARNING Switch off the mains power supply to the spectrometer wait 60 seconds and disconnect the power cable before you open the cover of the spectrometer This makes sure that you are safe from electrical Shock and laser radiation Due to its high operating temperature allow sufficient time for the source to cool before attempting to remove it 1 Open the main cover of the instrument as detailed on page 84 and identify the Source Figure 34 Figure 34 Source location CAUTION Caution hot surface The source housing and source itself will be hot if recently switched on Allow to cool before performing the following procedure The source element is brittle and can be broken if not handled properly Be careful when removing and installing the source Do not touch the glass bulb as this will degrade the performance of the bulb and shorten its life 68 Spectrum 100 Series User s Guide 2 Slacken the two terminal screws Figure 35 securing the wires leading to the source Disconnect the wires from the connectors 3 By grasping the round metal source body NOT the wires remove the source from its housing by pulling noting its installed position 4 Install the new source in its housing by pushing in until it clicks into place 5 Re
68. o examine Quality Check results in more detail select the appropriate scan then click the button 66 Spectrum 100 Series Users Guide Routine Maintenance 68 Spectrum 100 Series User s Guide Cleaning the Spectrometer Clean the outside of the instrument using a damp cloth If necessary a mild detergent may be used Before you clean the entire instrument always perform a patch test on an inconspicuous area Avoid spilling liquid into the instrument Clean all external spills immediately If anything that is spilled enters the main body of the spectrometer switch off the power and contact a PerkinElmer Service Engineer Do not touch or attempt to clean any optical surface in the instrument because this will impair its performance and may easily CAUTION damage the component Cleaning the Display Clean grease and dirt off the Display using a soft damp cloth and a mild detergent Routine Maintenance 69 Moving the Spectrometer The spectrometer is a heavy precision instrument so two people are required for safe handling Consult the local codes of practice issued by safety advisors before attempting to lift it WARNING Take care not to injure yourself or others or to drop the spectrometer Before moving the spectrometer switch off the power supply wait 60 seconds and disconnect the power cable WARNING The spectrometer can be lifted using the shaped handholds on its sides as shown in Fi
69. og tabs click Start The Scan Progress dialog opens Scanning sample press Halt to stop scan Sample ID Sample 001 PIE s 3L bd ef us inr Ls Alc be amp ee 4000 3500 3000 2500 2000 1500 1000 500 Name Description Sample 001 sp Description 1 LOD 2of4scans G6 Quality Passed J If for example the instrument resolution setting has been increased a valid background spectrum may not be available The Scan Progress dialog prompts you to ensure the beampath is clear so that a background spectrum can be collected Click Scan to begin The Scan button changes to Halt while the data is being collected 3 Ifa valid background spectrum is available or after a background spectrum has been collected the Scan Progress dialog prompts you prepare your sample 4 Click Scan to begin collecting your sample spectrum The Scan button changes to Halt while the data is being collected As each spectrum is collected it is checked off on the Multiple Sample table Scan and Instrument Setup File Setup Help QJEUS5 Multiple Sample Scan Instrument Beam Accessory Number of Samples 4 IV Preview Scan Name Description Sample 001 Description 1 Sample 002 Description 2 Sample 003 Description 3 Sample 004 Description 4 Using the Spectrometer 59 5 If Quality Checks have been set the selected tests are performed as each spe
70. on the following pages z BENE m FUSE 2AT LR 64912C This product conforms to ail applicable standards of 21 CFR Chapter 1 Subchapter J Part 1040 10 Center for Devices and Radiological Health GLASS LASER PRODUCT U S department of Health and Human Services at the date of manufacture BS EN 60825 1 1994 IEC 60825 1 1993 Figure 1 Labels rear of instrument NOTE The label with a crossed out wheeled bin symbol and a rectangular bar indicates that the product is covered by the Waste Electrical and Electronic Equipment WEEE Directive Refer to Appendix 3 WEEE Instructions for PerkinElmer Products 20 Spectrum 100 Series User s Guide Serial Number and CAUTION LASER RADIATION WHEN Date of Manufacture OPEN AND INTERLOCKS DEFEATED DO NOT STARE INTO BEAM NOTE There is a second copy of the CAUTION label inside the cover Figure 2 Labels sample area Product identification label I I PerkinElmer PerkinElmer precisely precisely Spectrum 100 Spectrum 100N FT IR Spectrometer FT NIR Spectrometer Figure 3 Product label front of instrument Warnings and Safety Information 21 EMC Compliance EC directive The Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers have been designed and tested to meet the requirements of the EC Directive 89 336 EEC The instruments comply with the E
71. ower However the instrument should be disconnected from the mains before opening the main cover for maintenance Under the cover is the optical system of the instrument Consistent reliable performance is achieved by having few moving or adjustable parts and by extensive insulation of the optical system from the effects of humidity and vibration Stability of the optical system The entire optical system is purged and sealed at the factory A supply of desiccant placed within the system removes any water vapor and carbon dioxide that may enter A humidity sensor is fitted which warns you when the desiccant needs changing In the Spectrum 100 FT IR KBr or CsI windows separate the sample compartment from the purged optical system In the Spectrum 100N FT NIR these windows are CaF You can purge the sample compartment with dry air or nitrogen Either one removes water vapor however nitrogen is preferable because it also removes atmospheric carbon dioxide Do not use a flammable gas to purge the Spectrum 100 FT IR or Spectrum 100N FT NIR spectrometers The instrument contains a hot source and a fire or explosion will result Only use clean dry oil free nitrogen or air to purge the instrument WARNING The optics are kinematically mounted to ensure accurate positioning and to make them rugged The interferometer is enclosed and mounted on anti vibration mounts to guard against air and bench borne disruptions The interferometer us
72. p dialog NOTE AVI correction uses a methane gas cell installed in the filterwheel AVI correction is only available on the Instrument tab if this cell has been fitted What is AVI correction The objectives of Absolute Virtual Instrument AVI correction are e Consistent performance over time and between instruments e Traceability for all measurements Although FT IR and FT NIR spectrometers use a reference laser the wavenumber calibration and lineshape are affected by differences in beam divergence and uniformity This is true for all FT IR and FT NIR spectrometers Differences can occur between instruments when using different sampling accessories and when components are changed AVI allows calibration and lineshape to be maintained The Absolute Virtual Instrument is an instrument with theoretical performance such that the result of measuring a known sample on such an instrument can be predicted So if we measure with a real instrument and calculate the software transform to match the theoretical result we can apply this transform to future measurements The Absolute Instrument is defined by wavenumber calibration instrument lineshape and ordinate accuracy What does AVI correction do When AVI is switched on the software measures the current instrument profile relative to an absolute standard the methane cell in the filterwheel and an ideal lineshape function and applies a correction The use of an on board methane
73. processing of their own waste products under the terms of the WEEE Directive Please contact these manufacturers directly before discarding any of their products Consult the PerkinElmer web site above for manufacturer s names and web addresses 116 Spectrum 100 Series User s Guide Index A About This Manual 6 Accessory Changing esi iiini raii 50 110 Release Handle ee 111 Advanced User 55 AVI Cell Installation eene 96 Orientation ccceccssecsessecseeeeeesseeseeeees 96 B Beamsplitter LOCatiOD ici ed cerro Leer sn t Ee crea a en ei ve 89 Replacing 2 eerte n nr 89 C Certified Reference Material 100 Cleaning Display etie cit ei deren 68 External dy e rer ees de eere eoe 68 Collecting Reference Spectra 101 Condensation 69 Conventions Notes Cautions and Warnings 7 E A E ES 7 Cooling 14 D Desiccant Changing in iia E 33 71 Disposable nnns 73 75 TrdiCator 3 Er 33 70 Rechargeable sseessss 75 Removed eicesceeee eiie rore ren 74 Replacement Warning 71 Stowe ain iare cie coe decine or d 75 Desiccant Cover Captive Screws 74 Desiccant Indicator 33 70 Display Cleaning eee iiic renis 68 E EMC Compliance sssini inridning 21 EC directive 5 tede 21 FCC r les eie co una curer ania a 21 Standard EN 61326 1 21 F FCC
74. r baffle and secure with retaining screws Close and secure the Main Cover steps 10 to 15 on page 86 Plug in and switch on the instrument Advanced Maintenance 91 Installing Replacing Windows The following procedure describes how to install a window in one of the external beam ports The details apply equally to windows in the sample compartment Switch off the mains power supply to the spectrometer wait 60 seconds and disconnect the power cable before you open the cover of the spectrometer This makes sure that you are safe from electrical Shock and laser radiation WARNING 1 Open the main cover of the instrument as detailed on page 84 e Using the hexagonal wrench supplied with the new window kit slacken and remove the screws securing the beam port cover or current window to the main cover e Remove the beam port cover or current window and seal Retain for further use Be careful not to get moisture on the optical surface of the window Do not touch or breathe on the optical surface of the window CAUTION 2 Ensure the seal is fitted to the window and is correctly seated Figure 38 Figure 38 Seal fitted to window 92 Spectrum 100 Series User s Guide 3 Fit the window to the main cover from the outside in Ensure the seal is fully seated and the key on the window lines up with the notch in the main cover Notch and key Figure 39 Window installed in Main Cover y
75. ra asin 109 Appendix 1 Changing the Sampling ACCESSOFY cceceesssseeeesasseeeeeaaaees 110 Appendix 2 Instrument Self Checks eene n 113 Appendix 3 WEEE Instructions for PerkinElmer Products 115 Introduction 6 Spectrum 100 Series User s Guide About This Manual This manual contains the following sections Introduction Warnings and Safety An Overview of the Spectrum 100 Series Spectrometers Unpacking and Installation Using the Spectrum 100 Series Spectrometers Routine Maintenance Advanced Maintenance Instrument Performance Validation Appendices For further information on collecting viewing and processing spectra using Spectrum software see the on screen HTML help file which you can access from the Help menu or by clicking Help on a dialog There is a multimedia introduction to the instruments which you can access directly off the Spectrum Multimedia CD This CD also includes multimedia tutorials for each of the main sampling accessories Introduction 7 Conventions Used in this Manual Normal text is used to provide information and instructions Bold text refers to text that is displayed on the screen UPPERCASE text for example ENTER or ALT refers to keys on the PC keyboard is used to show that you have to press two keys at the same time for example ALT F All eight digit numbers are PerkinElmer part numbers unless stated otherwise Th
76. s ssseeee 84 Opening eeina 84 Mains supply Fluctuations eeseeeeeeeeeee 13 MCT Detector Spectrum 100 COONING f ES 81 Multimedia CD 6 N NPL Standard 100 O Optical System Stability iiiaae err tere 27 P Purging connectors enr nnn nen ca 76 gas regulator pressure 77 purge gas lines ssseeeee 76 R Requirements Electrical TA 30 Environment ssseeeeeeeeeee 30 Minimum PC eeeeeeeeeeeene 38 S Safety Electtical ooo iere entere n 14 General ctio 13 Lase r e aaa S a E 16 Laser ApertUreS esssssrrssesrrrrrrreeeerrrene 18 Laser Radiation Hazard 17 Location and Ventilation 14 SUMMA sii es esee paper n esa turre rne 12 Sample Area Cover Release Clip enne 110 Removing seeee sadua 110 Sample Slide Knurled Screw eene 93 Sampling Accessories Diffuse Reflectance 28 FIATR eiie ethernet exa ene n 28 Liquid Sipper eene 28 NIR Fiber Optic Probe 28 NIR Tablet Autosampler 28 yc RR 28 Sample shuttle ssssss 28 Universal ATR eene 28 Sampling Accessory Changing issi sesiis 50 110 Installing New sees 111
77. s in a spectrum collected from your TRM are not within 0 3 cm of those given in the column labelled TRM on the Certificate of Performance Validation you should recalibrate your instrument as described in Wavenumber Calibration in the Spectrum on screen help NOTE The reference laser controls the wavenumber scale of the instrument and the scale is calibrated by measuring the effective wavenumber of the laser This differs from the absolute value because the infrared radiation in the interferometer is not all exactly parallel to the laser beam A different calibration is required for the Near Infrared Reflectance Accessory NIRA and remote accessories Any calibration error will be proportional to the wavenumber value You must check the calibration of your instrument if a new source detector or power supply has been fitted to your instrument and recalibrate if necessary For recalibration purposes we recommend that you use the peak at nominally 5669 3 cm in the column labeled Traceable Reference Material on your Certificate of Performance Validation 108 Spectrum 100 Series User s Guide Appendices 110 Spectrum 100 Series User s Guide Appendix 1 Changing the Sampling Accessory 1 Remove the sample area cover if fitted by opening the cover pressing the clip and pulling the cover vertically to remove Figure 50 Sample Cover Release Clip Figure 50 Removing the sample area cover Appendices
78. s securing the desiccant cover Figure 20 Figure 20 Captive screws securing desiccant cover 4 Open the cover and remove all the exhausted desiccant packs Figure 21 noting how they are installed Desiccant Compartment Figure 21 Desiccant removed Routine Maintenance 75 5 Place the first three packs of desiccant upright in the recess on the right of the desiccant holder then one at a time lay the three remaining packs flat in the upper part of the holder Figure 22 Figure 22 Stowing the desiccant packs Ensure that when the packs have been installed they do not rise above the level of the black rubber purge seal 6 Close the cover and tighten the screws 7 Refit the sample area cover Installing rechargeable desiccant There is also a rechargeable desiccant kit available L1250261 To install rechargeable desiccant packs follow the Renewing the disposable desiccant procedure starting on page 73 The instrument requires two rechargeable desiccant packs the packs are sized to fit into the left 70 mm x 78 mm x 33 mm or right 102 mm x 78 mm x 84mm of the desiccant container The standard kit contains two pairs of rechargeable desiccant packs This is so you can immediately replace the desiccant in the instrument with re activated packs The desiccant packs can be re activated by baking them in an oven at 250 C for approximately 8 hours
79. shipped with the instrument so that a spectrum can be collected and the position of five standard peaks checked 11 When the tests are complete click View Log A new window allows you to see the results of the instrument performance tests in more detail Unpacking and Installation 45 12 Click Next If your instrument is an FT IR Spectrometer the wizard now offers the opportunity to Collect ASTM Reference Spectra which are used when ASTM validation tests are performed For more information about ASTM level 0 tests see the HTML Help for the Spectrum software Spectrum Instrument Install Wizard 4 E Collect ASTM Reference Spectra Instrument Wizard Instrument In order to use the ASTMO tests within the Instrument Validation software you must collect a series of reference spectra ASTM Reference Spectra gt Collect ASTM Reference Spectra Collecting ASTM Reference Spectra Finished To continue with the collection of the spectra click Next To skip the collection press Skip Skip lt Back Finish e Ifyou do not want to collect ASTM Reference Spectra click Skip to move to the Finish page e If your instrument is an FT NIR spectrometer the Finish page is displayed 46 Spectrum 100 Series User s Guide 13 To collect ASTM Reference Spectra click Next Spectrum Instrument Install Wizard Collect ASTM Reference Spectra Instrument Wizard Select Instrument Collecting ASTMO r
80. spheric suppression can be set in the Advanced section on the Instrument tab of the Scan and Instrument Setup dialog What is atmospheric suppression This is an atmospheric correction routine This routine is more powerful than simple subtraction overcoming the following issues e Non linearity due to resolution e The measured spectrum is temperature dependent Lineshape and calibration are affected by J stop and sample or accessory What does atmospheric suppression do When CO2 H20 is switched on the software uses a single reference spectrum derived from high resolution data and our understanding of the instrument to model the lineshape then finds the current real instrument parameters by least squares fitting to the measured spectrum iiu s cda rr N B j V xt Difference IVA Ne wh MW Lah af Rr wy igh resolution reference 1740 1700 1660 1620 1580 1540 1500 0 Figure 13 Correction of a mid infrared spectrum at 4 cm resolution 62 Spectrum 100 Series User s Guide 46 6 45 EGY 40 no atmospheric suppression 34 0 8407 5 8000 7500 7000 6500 6000 5500 5000 4649 1 cm 1 46 6 45 EGY 40 with atmospheric suppression 34 0 6407 5 8000 7500 7000 6500 6000 5500 5000 4649 1 cm 1 Figure 14 Atmospheric suppression Using the Spectrometer 63 AVI Correction AVI correction can be set in the Advanced section on the Instrument tab of the Scan and Instrument Setu
81. tched on and connected to the PC The instrument may be connected directly to the PC using a suitable cross rence Spectra over cable or to the network using a suitable network cable If connected via the network the IP address and Subnet mask of the instrument must be set to values appropriate for your network The TCPAP settings of the PC must be set appropriately See the Administrators Guide for more details To continue click Next Cancel 40 Spectrum 100 Series User s Guide 3 Click Next The Instrument Details page is displayed Spectrum Instrument Install Wizard Instrument Details Instrument Wizard Select Instrument Enter the details for your instrument Configuration Instrument Name gt Instrument Details oT Configuration IP Address 3TM Reference Spectra Finished J J Use Factory Default 4 Enter an Instrument Name which will be used by the Spectrum software to identify your instrument 5 If the instrument is connected to your network enter a suitable IP Address which can be provided by your network administrator If the instrument is connected directly to your PC using the supplied cross over cable click Use Factory Default Unpacking and Installation 41 6 Click Next The Instrument Configuration Disk page is displayed which prompts for the lt name gt cfg of the configuration file required by your instrument This file is shipped on a CD with your instrume
82. that specifications are maintained after shipping act Instrument Install Accessories ling A To continue with the accessory installations click Next To skip the accessory installations click Skip Skip Finish Some accessories such as the Universal ATR but not all are shipped with a disk containing for example reference spectra generated during manufacture If required you will need this Accessory Disk at step 6 50 Spectrum 100 Series User s Guide 4 Click Next The Insert Accessory dialog is displayed Insert Accessory Please ensure that the accessory you wish to test is installed correctly in the instrument Current accessory Universal ATR Diamond ZnSe 1 Continue 5 Fit the accessory to be installed in the instrument as described in its User s Guide which is provided on the Spectrum 100 100N Manuals CD L1050002 Typically you remove the sample slide and sample cover from the instrument then slide in the accessory module making sure that it is properly latched and if appropriate that its top plate is in place See Appendix 1 Changing the Sampling Accessory on page 110 6 Click Continue If required the Copy Accessory Files page is displayed which prompts for the lt serial number gt cfg of the configuration file required for your accessory This file is shipped on a disk with your accessory Copy Accessory Files Some accessories are shipped with a configuration disk
83. till be charged even if the instrument has been disconnected from all voltage sources e The instrument must be disconnected from all voltage sources before it is opened for any adjustment replacement maintenance or repair Any interruption of the protective earth conductor inside or outside the instrument or disconnection of the protective earth terminal can make the instrument dangerous WARNING Warnings and Safety Information 15 The instrument has e An IEC Pollution Degree 2 classification usually only non conductive atmospheric pollution of the equipment occurs occasionally however a temporary conductivity caused by condensation must be expected e An IEC Insulation class I rating for external circuits only connect equipment that meets the requirements of IEC 61010 1 IEC 60950 or equivalent standards The instrument is designed to be safe under transient overvoltages typically present on the MAINS supply NOTE The normal level of transient overvoltages is impulse withstand Overvoltage category II of IEC 60364 4 443 16 Spectrum 100 Series Users Guide Laser Safety Regulations The Spectrum 100 FT IR and Spectrum 100N FT NIR spectrometers are CDRH Class I BS EN 60825 1 IEC 60825 1 Class 1 laser products The optical module contains a Class II 2 Helium Neon HeNe laser which emits visible continuous wave radiation at a wavelength of 633 nm and has a maximum output power of 1 mW Some o
84. tuazioni che potrebbero arrecare gravi danni allo strumento o ad altra apparecchiatura Troverete informazioni su tali circostanze in un riquadro come questo e Caution Opgelet Betekent dat de genoemde handleiding nauwkeurig moet worden opgevolgd om beschadiging van het instrument te voorkomen o Caution Atenc o Significa que a instru o referida tem de ser respeitada para evitar a danificac o do aparelho 8 Spectrum 100 Series Users Guide A WARNING We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons Details about these circumstances are in a box like this one Cp CED M e o Warning Warnung Bedeutet daB es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann Warning Advarsel Betyder at brugeren kan blive kveestet hvis anvisningen ikke overholdes Warning Peligro Utilizamos el t rmino WARNING PELIGRO para informarle sobre situaciones que pueden provocar da os personales a usted o a otras personas En los recuadros como ste se proporciona informaci n sobre este tipo de circunstancias Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels l utilisateur ou d autres personnes Les d tails sur ces circonstances sont donn es dans un encadr semblable celui ci Warning
85. uide 2 Carefully remove the instrument from the shipping container but not from the bag in which it was shipped The spectrometer must be allowed to reach the temperature of its surroundings before it is removed from the bag This means leaving it CAUTION overnight if it has been moved from a cold area and at least 4 hours after removal from the shipping container 3 When the spectrometer has been allowed to warm to the temperature of its surroundings remove it from the bag and place it on the bench where it is to be used Ensure that you can reach the rear of the spectrometer to enable you to connect the cables NOTE Any accessories will be shipped in separate boxes Unpacking and Installation 33 The Desiccant Indicator The optical system of the spectrometer is purged at the factory This protects the beamsplitter and the sample compartment windows from being damaged by humidity Replaceable packs of desiccant keep the optics dry and free of CO The top panel of the instrument includes a Desiccant Indicator whose sectors change sequentially from blue to pink as the desiccant packs are exhausted Change the desiccant in the instrument when the sector marked 10 becomes pink but while the sectors marked 15 and 20 are still blue Refer to Changing the Desiccant on page 71 Refer to 7he Desiccant Indicator in detail on page 70 for more information about the appropriate action to take if the sectors marked 15 and
86. uring screws 4 Place the assembly on the sample accessory baseplate Figure 43 Next available position Figure 43 Filterwheel assembly on accessory baseplate Identify the next available filter position remove the foam and filter holder place the filter in the hole and place the foam spacer on top of it Figure 44 illustrates the assembly sequence The next available filter position is the next empty position working clockwise as shown in Figure 43 Make sure there is at least one open beam position if possible QUS NN S5 Figure 44 Filter foam spacer and filter holder 5 Advanced Maintenance 95 7 Secure the filter to the filterwheel by clipping the holder into place Figure 45 Align the notches of the holder with the indents in the filterwheel then twist the holder clockwise to lock it Filter holder Figure 45 Installing the filter holder 8 Refit the filterwheel to its mounting and secure it in place with the two screws 9 Refit the sample slide 10 Close the main cover see page 86 steps 10 to 15 11 Plug in and switch on the instrument 12 Update your Spectrum configuration so that the software is aware of the new filter Refer to Filterwheel in the on screen help system 96 Spectrum 100 Series User s Guide Installing an AVI Cell in the Filterwheel Assembly Switch off the mains power supply to the spectrometer wait 60 seconds and disconnect the power cable b
87. w voltage Directive 73 23 EEC amended by 93 68 EEC If the instrument is used in a manner not specified by the manufacturer the protection provided by the instrument may be impaired Only use the instrument indoors and under the following conditions Temperature 15 C to 35 C Relative Humidity 75 maximum non condensing If possible avoid any adjustment maintenance and repair of the opened operating instrument If any adjustment maintenance and repair of the opened operating instrument is necessary this must only be done by a skilled person who is aware of the hazard involved Whenever it is likely that the instrument is unsafe make it inoperative The instrument may be unsafe if it e shows visible damage e fails to perform the intended measurement e hasbeen subjected to prolonged storage in unfavorable conditions e has been subjected to severe transport stresses Use of controls or adjustments or performance of procedures other than those specified herein may result in hazardous radiation exposure WARNING If the equipment is used in a manner not specified herein the protection provided by the equipment may be impaired The instrument has been designed to be safe under the following environmental conditions Indoor use e Altitude up to 2000 m above mean sea level e Ambient temperatures of 5 C to 40 C e A maximum ambient relative humidity of 80 for temperatures up to 31 C decreasing l
88. within 10 of the nominal voltage If possible do not connect the instrument to circuits that have heavy duty equipment connected such as large motors If possible do not use photocopiers discharge lamps radio transmitters and other equipment with large or frequent transient loads on the same supply circuit The primary fuse 2AT 250 V is in the drawer on the rear of the instrument next to the mains inlet the spare fuse is in the same drawer The primary fuse is connected in the live line Environment To obtain the best performance from your instrument e Place the instrument in an environment that is relatively dust free e Make sure that the bench top is free from vibration or mechanical shocks e Do not place the instrument or the PC near to room heating equipment such as central heating radiators e Do not position the instrument in direct sunlight as this may cause overheating e Leave at least 15 cm 6 inches from any surface and the cooling louvers at the rear of the instrument e Leave at least 7 cm 3 inches from any vertical obstacle to the sides of the instrument to permit an adequate flow of cooling air e Make sure that there are no overhanging shelves and no water pipes or faucets that could leak onto the instrument e The area near the PC must be free of strong magnetic fields direct sunlight and heating or cooling units or ducts The instrument has been designed for indoor use and operates correctly

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