GENE LAB SL6040A User's Manual BIOER TENOLOGY CO.,LTD
Contents
1. Chair Centrifuge Transfer Window Sample Facture Area PCR Detector Elum amp Reagent Preparation Area Q Fridge Air Flow Air Flow Air Flow Buffer Area I Buffer Area Buffer Area e ee ere LL Operation Procedure Picture 1 Arrangement of Gene Lab 1 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL There are clothes racks and trimness boxes used to change clothes and shoes in the three buffer areas Each Lab and buffer area has illuminating lamps UV lamps and ventilators in the top In Reagent Preparation Room there are experimental desk labets fridge mobile UV lamps transfer window that connects with Sample Preparation Room and chairs On experimental desk there are experimental equipments including centrifuge shaker pipettes centrifugal tubes waste lye urn a waste bin and so on In PCR Amplification Room there are an experimental desk labets a transfer window that connects with Sample Preparation Room and a chair On the experimental desk there are experimental equipments including Real time PCR Detection System computer printer waste lye urn waste bin and so on In suitable place of each area there are power outlets telephone outlets network outlets and air2. Remedy Some certain Illuminating 1 lamp does not work when press the switches Starter is broken Change starter Luminous tube is broken Change luminous tube Ventilator in PCR Total power is not connected Check the power and connect it Amplification does not work PL4 air switch is not connected Check the switch and connect it Motor of the ventilator is broken Change the motor Water faucets signal wires are Firm the signal unstuck wires There is no water after Chanae new turning on the water faucet Battery is run out S i Enhance Hydraulic pressure is not enough hydraulic pressure Change the UV 4 UV lamp in transfer window UV lamp s tube is broken lamp s tube does not work Power outlet is unstuck Firm the outlet 12 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL III Area II Area Socket Socket x ACinput a AC100 120V AC220 240V a 50 60Hz CR Breaker main 5 i Breaker PL1 PL2 a z sanz 63A 40A 50A 30A i Hl Hl DI e L2 o o L3 N mee N2 pes e N3 PE PE1 I Area Buffer Lamp co e D l LL Lamp Breaker Grounding PL3 Switch for Lamp G UI 16A 10A de e K1 1 oo L4 1 o2 I II Lamp L41 s N4 K1 2 s CAT lig eo i 3 Lamp L43 2 Ie 7 switch L44 Full Fa K3 1 i lge g2 SA apr een 45 Switch for UV Lamp K2 I lo 92 JA D UV lamp L48 K2 Bre
3. Turn off the ventilator K3 1 and keeping K3 2 in Power Run state thus the ventilators in Buffer area Buffer II area and PCR Amplification Room start to exhaust air to outside o NOTE Do remember to turn off K3 1 before experimentation PREPARATION OF REAGENTS Staffs should change work clothes in Buffer Area while wear gloves and cap in Reagent Preparation Room according to Work Rules of Reagent Preparation Room And then prepare reagents refer to The standard operation of Reagent Preparation Room put the prepared reagents into transfer window close the window turn on the UV lamps in the window working for 30 minutes in general refer to Standard Operation and Maintenance of Transfer Window take down the experiment notes in details and clean the experiment desk according to The Processes to Cleaning Lab Afterwards please change work clothes in Buffer Area I and close the door when leave o NOTE Please do not take out the work clothes and castoffs PREPARATION OF SAMPLES Please change work clothes in Buffer Area II while wear gloves and cap in Sample Preparation Room following Work Rules of Sample Preparation Room And then turn off the UV lamp in the transfer window take out the prepared reagents receive clinical samples finally prepare samples in biology safety labet refer to Processes of Sample preparation in Sample Preparation Room put the prepared samples into another transfer window tr
4. book and pen for recording SUIT OPERATIONAL PRINCIPLE OF GENE LAB 1 Operational principle of Reagent Preparation Room Reagent Preparation Room is a place for storing and preparing reagents what s more it is the cleanest place in the whole Gene Lab If reagents are polluted the PCR experiment produces will be incorrect surely Hence air should flow from Reagent Preparation Room to Buffer Area l to make sure reagents pure in process of storing preparing and transferring Besides should set a UV antisepsis system in Reagent Preparation Room and Buffer Area for antisepticising environment before start working After reagents prepared please divide flow of people and flow of materials transfer reagents between Reagent Preparation Room and Sample Preparation Room by the transfer window thus to protect reagents from pollution Please set a UV antisepsis system in transfer window and operate transfer window according to standard operation programs BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL 2 Operational principle of Sample Preparation Room Sample Preparation Room is a main experiment area in Gene Lab It includes procedures as below receiving samples sorting DNA RNA and storing in low temperature putting the templates reverse transcription into amplification tubes putting the prepared templates DNA RNA into main reaction mixture and so on As the samples are prepared in the biology saf
5. of Clinical Gene Lab in January 2002 The Methods made general prescripts of Gene Lab such as setting technique checking and accepting training surveillance and management etc It also requires all of the technicians who were engaged in clinical PCR should be trained and licensed before starting to work The Clinical Gene Lab is the place where the whole PCR experiment is operated Whether the settings of Lab are reasonable or not will affect the results of experiment so the Lab should be set according to Temporary Management Methods of Clinical Gene Lab which issued by Ministry of Health P R China Gene Lab is a Clinical Gene Lab whose specifications magnitude arrangement position setting job specification operation procedure and management system are definite and argued again and again by several experts It should also accord with Temporary Management Methods of Clinical Gene Lab This Lab adopts Automatic Real time PCR Detection System having three workarounds namely Reagent Preparation Room Sample Preparation Room and PCR Amplification Room These three workarounds are arranged ideally in this Lab and there is a buffer area in the front of each workaround as showed in Picture 1 3 3 J J T m T ispla Realfine PCR Detector M our d Chair we SSE Transfer Window Printer Constant Waste Lye Urn Biology Safety Cabinet temperature Shaker Bath Fridge Centrifuge
6. the UV antisepsis lamps K2 1 K2 2 K2 3 of area area II and area Ill and keep the lamps working for more than half an hour Refer to Standard Operation of UV Antisepsis 111 762 IL Area I Area Clean pu camp p Ventilator a Power Run K2 1 K2 2 k2 3 PL4 Illuminating Lamp Area TI Area I Area K1 1 K1 2 K1 3 PCR Amplification Room Sample Preparation Room IIT Area II Area cC ReagentP reparation Room 1 Area Flow BufferArea III BufferArea II BufferArea III Operation Procedure Picture 3 Panel of switches 9 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Q NOTE People should leave the Lab immediately once UV lamps are turned on to avoid body damage ZNWARRING During UV lamps working staffs are forbidden to enter Lab AIR EXHAUST Turn off the UV lamps K2 1 K2 2 K2 3 and mobile UV lamps and then turn the running K3 2 under Power Run and ventilator K3 1 under Clean state keeping all ventilators in each experiment areas and buffer areas exhausting air to outside for ten minutes before starting to operate o NOTE Please change work clothes before enter in each experiment area PREPARATION OF WORK
7. 3 CHARACTERS sss 3 SPECIFICATIONS 4 REQUIREMENT OF INSTALLATION 4 CHAPTER 3 OPERATIONAL PRINCIPLE sss 5 PRINCIPLES OF GENE LAB S SETTING 5 SETTING OF AIRFLOW S 0 0 rere ere erre rre rr enr 5 COLLOCATION OF EQUIPMENTS IN EACH WORKAROUND FOR 6 REFERENCE ONLY 6 OPERATIONAL PRINCIPLE OF GENE LAB Leer ree LLL LLL I 7 CHATER A OPERATION GUIDE eee erre rre err rre rre rrr n 9 APPENDIX 1 TROUBLESHOOTING AND REPAIR 12 APPENDIX 2 ELEMENTS DIAGRAM ce nre rre rne rrr nre 13 APPENDIX 3 WIRING DIAGRAM 1 e nne erre nre nr nr MTM 14 APPENDIX 4 WIRING DIAGRAM 2 ee nne rre rre rrr nr eM 15 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL CHAPTER 1 INTRODUCTION The technique of PCR is advanced in whole life sciences field However if we do not understand PCR technique comprehensively or have no relative quality measures it will lead operator to do test subjectively and stochastically thus it is possible to get false positive or false negative results As per characters of PCR technique and the requirements of clinical diagnosis Ministry of Health P R China issued the Temporary Management Methods
8. And in suitable place of each area there are telephone outlets network outlets and air condition pipeline outlets SPECIFICATIONS This Lab should comply with the specifications as below ITEMS SPECIFICATIONS REMARK Temperature difference between lt 3 C Lab and circumstance Total power lt 5kW Illumination power lt 600W Drive power lt 4400W Minimum illumination 23001x Noises lt 60dB REQUIREMENT OF INSTALLATION Please install the Lab under the following condition ITEMS SPECIFICATIONS REMARK Ambient temperature 18 277C Can fix air condition Ambient humidity 30 70 Can fix humidifier and desiccator Supply voltage AC100 120V AC220 240V 50 60Hz Inlet hydraulic 220kPa Minimum installation room m 6 0X4 5X3 0 LX W XH Flatness of ground lt 5mm 2m BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL CHAPTER 3 OPERATIONAL PRINCIPLE principLEs of Gene Lab s setting 1 Gene Lab could be divided into three workarounds 1 Reagent Preparation Room 2 Sample Preparation Room 3 PCR Amplification Room As mentioned in Chapter 2 Gene Lab has a special aisle in the front and the buffer areas of each experiment area are designed in one side of the aisle These three experiments line in turn as Reagent Preparation Room Sample Preparation Room and PCR Amplification Room respectively 2 Should set a clear
9. Before bring it into effect the modification should be proved for its rationality and authorized by branch director 5 WORKING PROCEDURE 5 1 Cleaning and disinfection tools suitable for each experimental area are all special but cannot be misused 5 2 Finishing experiment please use 4 84 disinfectant liquid or 10 sodium hypochlorite disinfectant liquid to clean the experimental tables and use ultraviolet lamp to do disinfection 5 3 In case samples or reagents overflow please use 1096 sodium hypochlorite to clean scour with the clear water and make relative records 5 4 Please hand the work clothes to the laundry room for cleaning and disinfection every week Pay close attention that work clothes in different areas cannot be washed in the same day 5 5 Please clean pipettors with 1096 sodium hypochlorite after daily use and carry on the 2 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL high pressured disinfection every two weeks 5 6 Before the daily work is over please turn on ultraviolet lamp to do ultraviolet disinfection for 60 minutes in the lab 5 7 Please clean the instruments and equipments every day o NOTE This procedure is for reference only BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL CONTENTS CHAPTER 1 INTRODUCTION eee nre ree rne nr rne rne nnne 1 CHAPTER 2 CHARACTERS AND SPECIFICATIONS LL
10. GENE LAB SL6040A User s Manual BIOER TENOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Note Bioer Co reserves the right to modify this manual at any time without notice Patent materials are included in this manual All rights reserved No part of this manual shall be photocopied reproduced or translated into other languages without written permission from Bioer Co Thank you for choosing our products Please read user s manual carefully before you use the lab at the first time Patent number ZL 2004 2 0109896 9 File number BYQ60140000000ESM File version JUNE 2010 6 4 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Important note 1 CONVENTION Note Please pay close attention to the important information in this column Failure to follow the advice in this column will possibly result in damage to or the malfunctioning of the equipments ZNWarring This symbol reminds user should be cautious when performing this operation procedure Failure to follow the requirements in this column could possibly result in personal injury 2 SAFETY During the operation maintenance or repair of Gene Lab the following safety measures should be taken Otherwise the safeguards provided by this lab are likely to be damaged the rated safety level to be reduced and the rated operation conditions to be affected Bioer Co shall not be in any way responsible for the consequences resulting from operator s not obser
11. LL N J I 23 LINE3 Lu z LINE5 0 I 0 I 0 I I 0 I H Qu fis oco 5 g P 2 Bu D 54 FRONT PANEL ER 9 2 x 5 a 0 EH E 58 E Note BACK side View P ga EE ER SC bo p 4 4 0 O LINE4 SN 1 38 GH O 4 5 SD EH 1 PE H w U Q K1 1 K1 2 LAMP Switch I AREA LAMP Switch III AREA LAMP Switch II AREA t LINE3 L41 14 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Appendix 4 WIRING DIAGRAM 2 Socket J1 J27 8 a 8 E a 28 H nm g a panel dy I dy x x o e a a E A P A mz 32 ag aa 9 S S 1 i B Air flow Air flow Air flow e path normal Path normal 2e ve Path normal X Crust H ground D lt T e a L gt a 2 L ly M mC Electrics Box Tr Sech N t LO CO 3 3 88 88 HUB1 Internet L III BUFFER L II BUFFER L I BUFFER EM mn E PcrLab ElectricsParts ubiety view Telphone wiring 15 BIOER TECHNOLOGY CO LTD
12. ND FOR REFERENCE ONLY 1 Reagent Preparation Room NO EQUIPMENTS UNIT QUANTITY 1 Fridge PCS 1 2 Centrifuge PCS 1 3 Blending machine PCS 1 4 Minim pipette SUIT 1 5 Mobile UV lamp PCS 1 6 Special work clothes SUIT 2 7 Indoor GE and PCS 4 8 Fridge thermometer PCS 2 9 Centrifugal tube CASE 1 10 Waste lye urn PCS 1 m Seier SUIT 12 Waste bin PCS 1 13 Special aes a pen for SUIT 2 Sample Preparation Room NO EQUIPMENTS UNIT QUANTITY 1 High speed centrifuge PCS 1 2 Blending machine PCS 1 3 Cooling amp Heating Block PCS 1 4 Biology safety labet PCS 1 5 Fridge PCS 1 6 Minim pipette SUIT 1 7 Fridge thermometer PCS 1 8 Mobile UV lamp PCS 1 9 Indoor rud and PCS 4 10 Centrifugal tube CASE 1 11 Waste lye urn PCS 1 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL 12 Consumables such as SUIT 2 gloves 13 Waste bin PCS 1 14 Special book and pen for SUIT 2 recording 15 Special working clothes SUIT 2 3 PCR Amplification Room NO EQUIPMENTS UNIT QUANTITY 1 Real time PCR Detection PCS 4 System 2 Minim pipette SUIT 1 3 Mobile UV lamp PCS 1 4 Indoor thermometer and PCS 4 hygroscope 5 Special working clothes SUIT 2 6 Computer PCS 1 7 Printer PCS 1 8 Waste lye urn PCS 1 9 Waste bin PCS 1 10 Consumables such as SUIT 2 gloves Special
13. aker tga g2 H H v tame L47 PLA K2 II ige g ili D UV lamp L46 16A 10A o o L5 Ee a N5 Appendix 2 Elements Diagram ti 0 Dei I Area 2 Socket ri 3 o ER E u oO e S11 Lan Internet 0 51 4 Socket II Area Buffer III Area Buffer FAN FAN Lamp Lamp N N N Wi a ur u u Ls s s S 5 Es 5 Ww Ww Ww Ww Ww ect um DE zo 2 TEE 2p 2 shan I Area Buffer II Area Buffer III Area Buffer UV Lamp UV Lamp UV Lamp Socket for Tel amp Lan II AREA SOCKET I AREA SOCKET S12 S13 ke E PE N I BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Appendix 3 WIRING DIAGRAM 1 TO Electrics Box UV LAMP Switch From Client s K2 I K2 II K2 III AC input 2 Ai Fan run Switch A 4 l LINE3 Full FAN switch ul L45 E z Oo TO Electrics Box E j E E e E E POWER INPUT JUNCTION BOX i e 5 Full Fan ON On Top or back side wall x x o I II III BUFFER L I II AREA Oo T N co ml ml om S 5 9 5 Half Fan OFF 9 Z Z il z 1 m INII BUFFER a a 4 Saas 5 amp g g a a 5 5 5 5 5 B 5 8 LINE3 1 x tjt 0 8 TTT TT Los S e OQOOOOOOOOO0QO0OQ0 LINEA HT 0 Ww E zd Z D 5 e
14. ansfer them to PCR Amplification Room close the window turn on the UV lamp in the window working for 30 minuets in general refer to Standard Operation and Maintenance of Transfer Window take down the experiment notes in 10 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL details and clean the experiment desk according to Cleaning Process of Gene Lab Afterwards please change work clothes in Buffer Area II and close the door when leave o NOTE Please do not take out the work clothes and castoffs ZNWARRING After enter in Sample Preparation Room remember do not turn back to Reagent Preparation Room PCR AMPLIFICATION AND ANALYSIS Please change work clothes in Buffer Area Ill while wear gloves and cap in Sample Preparation Room according to Work Rules of Sample Preparation Room Turn off the UV lamp in transfer window take out the ready made samples to do PCR amplification and produces analysis Afterwards take down the experiment notes in details and clean the experiment desk according to Cleaning Processes of PCR Lab please change work clothes in Buffer Area IIl and close the door when leave o NOTE Please do not take out the work clothes and castoffs ZNWARRING After enter in Sample Preparation Room remember do not turn back to Reagent Preparation Room 11 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Appendix 1 Troubleshooting and Repair No Trouble Trouble Analysis
15. condition pipeline outlets BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL CHAPTER 2 CHARACTERS AND SPECIFICATIONS CHARACTERS Gene Lab has several obvious characters as following 1 Ideal arrangement configuration Adopting Automatic Real time PCR Detection System in the Gene Lab we set three workarounds namely Reagent Preparation Room Sample Preparation Room PCR Amplification Room and the corresponding Buffer Area Buffer Area Il and Buffer Area Ill Let s arrange experiment areas and buffer areas as showed in picture 1 so that experimenters could change clothes and shoes in buffer areas before they enter into experiment areas while reagents and samples could be passed directly through transfer window from Sample Preparation Room to PCR Amplification Room 2 Standard requirement of air pressure s setting In order to avoid reagents and samples being polluted by aerosol and staff and circumstances being polluted by amplification produces during the whole PCR experiment there is certain differential air pressure set in every experiment area of Gene Lab Make sure safety of persons circumstances and samples on establishment basis 3 Precise measures of protection 1 There is a biology safety labet in Sample Preparation Room which is used for sample preparation The ventilation system in the labet can protect people and samples from being polluted by aerosol thus to ensure safety of people and samples In biol
16. ety labet please control the airflow inside of the biology safety labet to protect operators and samples The setting of airflow in this area is the same as that of Reagent Preparation Room and the air should flow from Sample Preparation Room to Buffer Area II There are also UV antisepsis systems in Sample Preparation Room and Buffer Area II After the samples are prepared they will be transferred to PCR Amplification Room through the transfer window from Sample Preparation Room 3 Operational principle of PCR Amplification Room PCR Amplification Room is the area where PCR amplification and amplification produces analysis are carried on It is the area with most serious pollution in the whole Gene Lab Hence please guarantee air flow from Buffer Area to PCR Amplification Room and install UV antisepsis systems in PCR Amplification Room and Buffer Il Area thus to avoid pollution to people and environment BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL CHATER 4 OPERATION GUIDE Please enter every area of the Gene lab following the single direction Reagent Preparation Room Sample Preparation Room PCR Amplification Room The process of the whole operation is listed as below ANTISEPSIS 1 Turn on the illuminating lamps K1 1 K1 2 and K1 3 that are shown in picture 3 2 Move the mobile UV antisepsis lamps to target place such as experiment desk then turn on and then close each door 3 Turn on
17. ogy safety labet the cleanliness reaches 100 grades 2 On the top of each experiment area buffer area biology safety labet and transfer window there is a UV lamp for antisepsis 3 In Reagent Preparation Room and Sample Preparation Room there are mobile UV lamps for local antisepsis of experiment desk 4 Compendious and clean appearance SL 6040A Gene Lab is made up of colorized steel plate aluminum alloy shaped section glass and so on and it is supported by aluminum alloy round column Its inner angles are decorated by inside radius aluminum alloy all of these allow the Lab to own firm configuration classic lines perfect appearance and excellent leakproofness 5 Modular design All of the parts are debugged in the manufactory and assembled in locale conveniently For this Lab the capacity is huge the production is efficient and the quality is credible so that the cost can be controlled efficiently 6 Standardization design BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Set standard dimensions of Lab to save room thus to satisfy different customers requirements 7 Patent products This item had gained patent in P R China whose number is ZL 2004 2 0109896 9 8 Centralized control All of switches are fixed in a faceplate for centralized control 9 Humanization design Some suitable place of experiment desk transfer window flume and hydrant in each of experiment area is designed friendly
18. sign each for all workarounds so as to avoid mix using equipments and things among different workarounds 3 When enter into each workaround please follow a single direction as Reagent Preparation Room Sample Preparation Room PCR Amplification Room 4 Should use different work clothes in different workarounds and should not bring them out when leave SETTING OF AIRFLOW S DIRECTION In order to avoid the amplification produces being polluted by environment and to meet the requirements of setting Clinic Gene Lab the airflow direction should follow the way as below Reagent Preparation Room to Buffer Area Sample Preparation Room to Buffer Area II Buffer Area Ill to PCR Amplification Room refer to Picture 2 Therefore please keep Buffer Area I Buffer Area II and PCR Amplification Room under negative air pressure during operation While keep other areas air pressure as 0 or You can turn on the ventilators in Buffer Area Buffer Area II and PCR Amplification Room to exhaust air There are controllable air gaps on the outside walls windows and doors of experiment areas so as to let fresh air enter indoor PCR Amplification Room Sample Preparation Room Reagent Preparation Room iN X OW E N A Special Aisle gt Operation Procedure Picture 2 Ideal arrangement of Gene Lab 5 BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL COLLOCATION OF EQUIPMENTS IN EACH WORKAROU
19. ving the following requirements a Grounding A C power s grounding should be reliable to safeguard against an electric shock The 4mm hard wires are used in the lab s general electric source and the general wire should be educed from the power supplier of the building to ceramic tie in in the lab b A C Power Considerations Always make sure that the main voltage conforms to the required voltage and the main rated power no less than the required power 5000W before the power is connected 3 MAINTENANCE Please refer to the Annex for the maintenance of the lab ZNWarring During the cleanout of operation desk power socket fans and lamps power should be cut off Its forbidden to drop abluent into operation desk and the holes of power socket It s forbidden to wash operation desk and wall with caustic abluent BIOER TECHNOLOGY CO LTD SL6040A GENE LAB USER S MANUAL Appendix Cleaning Procedure for Gene Lab 1 PURPOSE In order to guarantee neatness of lab s surroundings and prevent contamination 2 APPLICATION SCOPE Be suitable for cleaning and disinfection work to the lab s working surroundings experimental tables work clothes pipettors etc 3 RESPONSIBILITY All staff in the lab should know very well and observe this procedure the clearing work can be carried out by assigned personnel 4 MODIFICATION ON THE PROCEDURE Any staff who use this procedure can bring forward the modification of the procedure
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