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Wash8to12 Microplate Washer User's manual

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1. to abort during washing procedure 20 Wash8to12 User s Manual 4 2 System settings By double clicking the CANCEL key the System Setting mode is entered and the display is as follows Adjusting Contrast Changing Wash Head Changing Plate Adjusting Wash Head Adjusting Top Aspr Adjusting Pump By pressing the SELECT key you can change between the setting items By pressing the START key you enter the according submenu of the selected item 4 2 1 Adjusting Contrast By pressing the or the key the contrast of the display is changed according to the user s needs Press the START key to save the changing or the CANCEL key to ignore them 4 2 2 Changing Wash Head Changing Wash Head 21 Wash to12 User s Manual 22 Wash8to12 User s Manual By pressing the or the key you can change between 8 or 12 pin wash head Press the START key to save the changing or the CANCEL key to ignore them 4 2 3 Changing Plate Changing Plate Flat Bottom By pressing the or the key you can select either wells with flat bottom V bottom or Round U bottom Press the START key to save the changing or the CANCEL key to ignore them 4 2 4 Adjusting Wash Head Due to the fact that the dimensions of the microplates on the market are not uniform the Wash8to12 provides the function of dispensing head position adjustment Users can adjust the position of the dispensing head
2. 1 Wash8to12 User s Manual 11 Wash8to12 User s Manual 2 4 System Description 2 4 1 Hardware Components The hardware components of the Wash8to12 Microplate Washer are shown in figure 1 KeyPad Display Panel Dispense TubeAspiration Tube ash Head Handle f TOP Area Wash Head e ET ll Figure 1 The Wash8to12 system Wash Head The wash head contains a set 1x8 or 1x12 of dual wash pins Each set of wash pins includes a dispense pin and an aspirate pin They are closely spaced so that the fluid can be aspirated from a well while fluid is dispensed into the same well The wash head moves back and forth to wash each column or row depending on the wash head used of the plate by lowering the wash pins into the corresponding wells Display Panel The instrument communicates with the user via the display All system information and wash parameters entered by the user are displayed Keypad The keypad is used by the operator to select commands and to enter the wash parameters RS 232 Interface The instrument includes an RS 232 interface so that an external computer can be connected for technical service purposes in the future The RS 232 interfaced may also be used for updating new software versions 12 Wash8to12 User s Manual Wash Bottle The wash bottle contains the wash fluid for dispensing filling and purging with a dispense pump inside Waste Bottle The waste bottle with level sensor inside
3. collects the fluid aspirated from the wells Attention Cautions There is infectious risk of to touch the waste bottle when the instrument is washing Microplate User must be equipped with glove to touch waste bottle at the time of disposing of waste liquid Pay attention to guard against the bottle waste liquid leakage Connections Connectors at the rear of the RT2600C system are shown in Figure 2 Connections for the power cord the dispense pump power connector the waste bottle level sensor the waste bottle vacuum line and the external computer are shown at the rear of the panel Power Cord Dispense Pump Waste Bottle Power switch Connector Power Connector Tube Connector Baap Vacuum RS232 Waste Bottle Wash Bottle Tube Exhaust Interface Level sensor Figure 2 Rear View of the Wash8to12 system 13 Wash8to12 User s Manual 3 INSTALLATION 3 1 Unpacking Carefully unpack the instrument and remove it from the plastic bag Report any visible damage to your shipper or freight carrier at once Place Wash8to12 on a flat working surface Place the wash bottle and waste bottle behind Wash8to12 Remove the power cable and other items from the packing carton Please retain the packing material for future use 3 2 Installing the Wash Bottle Connect the white head with the pump Figure 3 Pump Let the pump power cable and the dispense tube go through the hole on the
4. an 14 3 3 Installing the Waste Bottle 0 ee ccesecseesseeseeseceeeeeeeseesecseeeseeaecseceeeaesaeceseeeeaeeaeeeeeeaees 15 3 4 Installing the dispensing head e ee eeeeeeceseeseceeeeeeeseesececeeseesecseceeeeseesececeeeeaeeeeeeeenees 15 3 5 Turnon tne systein anne area small Bae nea 15 4 OPERATION een 16 4 1 F nction description oerien sua cdubes ERE E E E EE A a OTE EE 16 4 1 1 Keyboatd nsn tesneni rae aet cheeses cnet EE A EN e e T ANEI CESE ee 16 4 1 2 Dispense onran a R E R E R RN ein lebens eE E a E RE 17 4 1 3 Aspiration e PnP En Oe ee eee 18 4 1 4 Startthe pr gramMer a nennen ei eE E Ee nae RAe oraaa ARAE Hae raae a ENEE 19 4 2 System Setting Ssss ccana a a a a A E 21 4 2 1 Adj sting Contra Ster ERER E E E RR R RR ARR 21 4 2 2 Ghanging Wash Head 2r sesseieeessfsesnesene frau ania teh aia 21 4 2 3 Changing Plate ee t aeea aerei anea e ee e aea e ee a a ER 23 4 2 4 Adjusting Wash Head u 0 eisen a a e 23 4 2 5 Adjust the horizontal of the wash head sssessessenensenenneenensenennnnnnennensennnnnnnnnennennenen 24 4 2 6 Adjusting Top Aspiration 25 2 28a ate esc neun nn ie pre u ein evans 24 4 2 7 Adjusting Pump 5 83 222 Era E AE E ine 25 5 MAINTENANCE AND CLEANING 2 asus aa 26 5 1 Daily maintenance of Worktable 22020eesensenseneenensensnnennennensnnennnnensenennensnsennnen 26 5 2 Daily maintenance of dispensing head 2nens
5. color code Waste bottle treatment The sensors in the waste bottle can sense the liquid height If the waste liquid reaches a certain height the software of the Wash8to12 detects the change of the sensor signal and stops any operation that involves liquid suction At this time please dispose the waste liquid timely which can be done without disconnecting the electrical source Hold the top of the waste bottle with one hand and lift it slightly Hold the body of the waste bottle with the other hand and turn clockwise until the top is detached from the bottle body Pour away the waste liquid Put the tube endings and the sensor back into the waste bottle and close it by turning the bottle counterclockwise Maintenance of the wash bottle Fill up the wash bottle with distilled water to wash the blank plate once a week and clean up the whole pore path and the diving pump Note The water level in the wash bottle should be higher than the diving pump inside to avoid the aspiration of air 27 Wash8to12 User s Manual 6 TROUBLESHOOTING List of malfunctions Problem When switching on the instrument no display appears and the instrument does not start up The instrument does not suck up the liquid and the vacuum pump does not work The instrument does not dispense liquid The liquid is out of control and dispensed all the time What to do Check the electric supply socket and fuse Check
6. Qualification To install maintain and repair the instrument a service technician has to be trained on the instrument by the manufacturer or their representative A service technician is also expected to be familiar with the normal operation of the instrument as described in the User s manual and the special operations as described in the service manual Electrical To use washer safely pay attention to the following items To prevent the risk of electrical shock and or damage to the instrument Operator should not open the cover of the instrument Only authorized personnel for example service technicians may open the instrument to perform maintenance or repair Touching the main board when the power is on may cause severe injury or death Any problem please ask for helps from your supplier Mechanical There is no risk presented by the mechanical parts of the instrument when the instrument is closed If the covers of the instruments are removed mechanical parts could cause personal injury or the instrument may be damaged if the following advice is not being considered DO NOT wear loose garments or jewellery that could catch in mechanisms DO NOT put your fingers hands into the pathway or any part while the instrument is in operation DO NOT attempt mechanical repair unless the instrument is not in operation or OFF Waste bottle There is infectious risk of to touch the waste bottle when the instrument is washing Microplate User m
7. Wash8to12 User s Manual Wash8to12 Microplate Washer User s manual Le gt BGT BioGenTechnologies BGT BioGenTechnologies GmbH Von Langen Weg 10 D 48565 Steinfurt Tel 02551 4090 Fax 02551 1298 Web www biogentechnologies de E Mail info biogentechnologies de Rev 3 4e Wash8to12 User s Manual CONTENTS SYMBOLS DESCRIPTION is ennon aa HR 3 SAFETY PRECAUTIONS AND POTENTIAL HAZARDS nenessenneseensennennnn 4 ADDITIONAL PRECAUTIONS ua eu 5 1 GENERAL INFORMATION sushi 6 1 1 How to use this manual rail 6 1 2 Gopyrighti rn sau R SD A eli deb bateceeebe beaten 7 1 3 Property Of ciecciiccdvcetls dvesdl ea eh dhisstdeadeastiaadiaetls died disth diet distaditnadin 8 1 4 VORAN ya tice Wilson a hele bea alles voout die Seststeduen sh adatastaved wacaustacertabedeareuctstostiestentben 9 2 USEAND FUNGTION nina na aain 10 2 1 Name and intended use ueeceeeseesssesseessnensnensnnnsnnnsnnnsnnnnnnnnnnnsnnnsnnnsnnnsnnnssnnsnnnsnensnensnensnenn 10 2 2 Product Feat resi 2 5 20248 a a AR ek ie HR 10 2 3 SPECINICALONS 3 rare EERTE ETIE A OT ink 11 2 4 System DesciiptioN eaen e a a ME e e e a e esehdns 12 2 4 1 Hardware Components ccceccscsssessesessesseseseeseeseeseeeesecseeseeseeaeeseaesecneesecseeseaeeeeesecseeaeeaeeaeeeeeenees 12 3 INSTALLATION ss eines 14 3 1 Unpackinig sr i E E Ce ls ul ee As ae 14 3 2 Installing the Washi Bottle enter eeneneernenne
8. e the power cord out of its bag Connect one end to the RT 2100C and the other to a main power socket Press the power switch on the rear side of the instrument After a series of self tests the software version interface will display Attention Cautions Safety is most important Please note the safety precautions and potential hazards mentioned above chapter Before operate the instrument You should also consider the common safety regulations of your laboratory Check the instrument s connection Make sure that the power cord is connected to the power supply and to the instrument Handle the waste correctly Make sure that the waste tubing on the instrument is connected to the waste bottle Avoiding contaminating yourself your environment and the instrument with biohazardous material 15 4 OPERATION 4 1 Function description 4 1 1 Keyboard The keyboard is mainly used for the input of all instructions and to convert the display signals Figure 5 shows the keyboard layout PRIME DISP Figure 5 Wash8to12 Keyboard Prime By pressing the PRIME key the filling process is initiated Rinse By pressing the RINSE key the rinse procedure is initiated Wash8to12 User s Manual Function of each button PRIME Start Prime procedure RINSE Start Rinse procedure DISP Start Dispensing procedure ASPR Start Aspiration procedure SELECT Change edit
9. ensnnnennensenennnnnnsenenennsennnen 26 5 3 Waste bottle trsatment 22 2222 Rs Ruin bi 27 5 4 Maintenance of the Wash bottle ssssssesnessessnsnennennensnnnnnnennnsnnnnennenenennn 27 6 TROUBLESHOOTING Eau 28 T PICTURES OF SOME SPARE PARTS usesessessensnsnensnssnsonsnnennnnnnnn 29 Wash8to12 User s Manual SYMBOLS DESCRIPTION Symbols in the manual NOTE Notes contain additional information or tips when using the instrument ATTENTION Cautions oo Cautions should be followed carefully to ensure your instrument work correctly and to avoid unnecessary personal injury Symbols on the instrument This means that the labeled item could lead to personal injury and or damage to the analyzer The symbol is labeled beside the power outlet and some external interface SN The symbols for SERIAL NUMBER the serial number shall be after or below the symbol adjacent to it IVD The symbol means the product is in vitro diagnostic medical device Wash8to12 User s Manual SAFETY PRECAUTIONS AND POTENTIAL HAZARDS General Before you start installing and working with the washer you should read the safety precautions and regulations shown in this chapter Operator Qualification Please note that the operation with Microplate Washer should be carried out only by the doctor or clinical inspectors who have undergone necessary training provided by the sales agent Service Technician
10. item rt h START Start wash procedure Go to next operation Cancel current operation CANCEL Double click to enter system setting Change parameter Change parameter 16 Wash8to12 User s Manual 4 1 2 Dispense By pressing the DISP key the dispensing procedure is initiated The display panel is like follows Fluid Volume 350uL By pressing the or the key you can change the dispense volume by a 50 uL step After the fluid volume is defined press the START key The display shows the following information Strip Setting Pressing the or the key defines the number of rows e g 1 to 12 to be dispensed By choosing Strip Select and pressing the START key you can define by pressing the or the key which of the rows of the plate should be dispensed Strip Setting By pressing the SELECT key the desired row is marked 17 Wash8to12 User s Manual 4 1 3 Aspiration By pressing the ASPR key the aspiration procedure is initiated The display panel is as follows Aspiration Single By pressing the or the key you can choose between single or double aspiration Press START and the following display appear Strip Setting Pressing the or the key defines the number of rows e g 1 to 12 to be aspirated By choosing Strip Select and pressing the START key you can define by pressing the o
11. nual Note This equipment is not intended for family usage This equipment must be operated by skilled trained clinical personnel A Warning A It is important for the hospital or organization that employs this equipment to carry out a reasonable maintenance schedule Neglect of this may result in machine breakdown or injury of human health Upon request BGT may provide with compensation necessary circuit diagrams calibration illustration list and other information to help qualified technician to maintain and repair some parts which BGT may define as user serviceable Wash8to12 User s Manual 1 4 Warranty Workmanship amp Materials BGT guarantees new equipment other than accessories to be free from defects in workmanship and materials for a period of 13 months from date of shipment under normal use and service BGT obligation under this warranty is limited to repairing at BGT option any part which upon BGT examination proves defective THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE Exemptions BGT obligation or liability under this warranty does not include any transportation or other charges or liability for direct indirect or consequential damages or delay resulting from the improper use or application of the product or the substitution upon it of parts or accessories not approved by BGT or repai
12. ponents of the Wash8to12 whose condition has an influence on the accuracy of the amount of dispensation as well as the amount of remnant liquid Therefore the maintenance of the dispensing head is of high significance The maintenance procedure is as follows Switch off the electric source of the machine Remove the tubes by twisting counterclockwise Put the dispensing head from the supporting arm Remove the two metal screws on the front side of the dispensing head Use the small sized needle to clear out each dispensing needle short thin needle and use the large sized needle to clear out each liquor suction needle long thick needle Take the dispensing head with the screw removed end downward Knock it against a paper towel or gauze so as to remove the blocking from the tubing Put the O ring around the root of the metal screws and tighten the two screws up Note the O rings must be put around the screws so as to guarantee hermeticity Fill up a large container with distilled water and immerse the dispensing head completely in the water Plug a large size syringe into the plastic hole on the dispensing head and push the syringe back and forth for several times Both plastic holes will be treated this way Mount the dispending head back onto the supporting arm and tighten up the plastic tubes clockwise Note assure that the tubes are connected according to their 26 5 3 5 4 Wash8to12 User s Manual
13. publication BGT intends to enforce its rights to this work under copyright laws as a published work Those having access to this work may not copy use or disclose the information in this work unless expressly authorized by BGT to do so All information contained in this publication is believed to be correct BGT shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material This publication may refer to information and protected by copyrights or patents and does not convey any license under the patent rights of BGT nor the rights of others BGT does not assume any liability arising out of any infringements of patents or other rights of third parties Content of this manual is subject to changes without prior notice Wash8to12 User s Manual 1 3 Property of BGT BioGenTechnologies GmbHGT Von Langen Weg 10 D 48565 Steinfurt Tel 02551 4090 Fax 02551 1298 Web www Biogentechnologies de E Mail info biogentechnologies de Responsibility on the manufacturer party BGT is responsible for safety reliability and performance of this equipment only in the condition that e All installation expansion change modification and repair of this equipment are conducted by BGT qualified personnel e Applied electrical appliance is in compliance with relevant National Standards The instrument is operated under strict observance of this ma
14. r the key which of the rows of the plate should be aspirated Strip Setting By pressing the SELECT key the desired row is marked 18 Wash8to12 User s Manual 4 1 4 Start the programmer The programmer is started by double clicking the START key The display shows as follows Wash Mode Plate Wash Times 1 Aspiration Single Fluid Volume 350uL Soak Time 00h00m00s By pressing the SELECT key you can choose the parameter that needs to be changed according to your proposed washing procedure Wash Mode Plate whole plate e Strip row by row Wash Times From 1 to 12 times Aspiration e Single or double Fluid Volume Tobe changed in steps of 50 uL by pressing the or the key Range 50 uL 3000 uL Note Select the fluid volume according to the volume of your Microplate wells to avoid excessive consumption of liquid Soak Time e By using the SELECT and the or the keys the desired soak time is set 19 Wash8to12 User s Manual Press START Key and the following display appears Strip Setting Press START to start wash procedure Press to enter the strip designating window press to select the designating strip then press select to confirm of exit When the cuvette following is empty it mean that the instrument will not dispense this strip Press START once again to start wash procedure Your can double press CANCEL
15. red by anyone other than a BGT authorized representative This warranty shall not extend to any instrument which has been subjected to misuse negligence or accident any instrument from which BGT original serial number tag or product identification markings have been altered or removed or any product of any other manufacturer Safety Reliability and Performance BGT is not responsible for the effects on safety reliability and performance of the Wash8to12 Microplate washer if Assembly operations extensions re adjusts modifications or repairs are carried out by persons other than those authorized by BGT Personnel unauthorized by BGT repairs or modify the instrument Wash8to12 User s Manual 2 USE AND FUNCTION 2 1 2 2 Name and intended use The Wash8to12 Microplate Washer is a microprocessor controlled Microplate washing system that performs wash protocols defined by the user The Wash8to12 is designed to wash all of the wells in one column or row of a 96 well plate at once The washing protocol can be programmed so that either all of the columns or rows are washed in the same manner or different wash cycles can be applied to specific columns or rows on the plate Product Features English operating system with large display for easy operation Suitable for flat bottom U and V shaped micro plates or micro strips including 8x12 12x8 8x6 or 12x4 micro plates Large storage capacity up to 50 different wa
16. shing programs can be edited and stored Automatic check of vacuum pressure to avoid jamming of the tubings e Automatic positioning which enables to rinse different plates Manual fine tuning ensures accurate positioning Liquor surface induction system with a liquor level alarm function and double needle design to reduce cross contamination e Easy programming The number of rinse the amount of liquor and the soaking time can be set according to user s needs Plate washing line washing and skipping rinse are at your option Rinse pressure and the amount of liquor are adjustable to reduce air bubbles and to ensure thorough rinse Automatic successive rinses and 8 path or 12 path rinse heads are interchangeable 10 2 3 Specifications Operation Wash head Display Net weight Storage Bottle Capacity Liquor Contact Medium Dimensions W x D x H Power Supply Power Consumption Fuse Environmental requirements Operating Range Transport and storage environments Computer Interface Interface Baud rate Parity Stop bit 8 channel or 12 channel 5 LCD 8 5 KG 2000 ml both washing liquor and waste bottle glass nylon poly tetrafluorine ethylene polypropylene stainless steel Tygon tubing 390mm L x330mm W x177mm H AC220V 10 50 60Hz 110V on request 80 VA SA 250V 05x20 10 C 30 C relative humidity lt 70 20 C 50 C relative humidity lt 93 Serial RS 232 port 19200 Odd
17. the power supply to the control board for 5V 12V and 24V input Check the power supply for 24V output Check the dispensing head for clogs and the electromagnetic valve for malfunction Check the electromagnetic valve for malfunctions If the problems cannot be solved please contact your dealer or distributor for further assistance List of spare parts CODE 12310570 12320070 22000030 12310101 12310100 12310080 1231054 1 12310530 12311170 NOTE 1231054 1 12310530 12311170 21000060A DESCRIPTION RT 2600 cover LCD display Main board Non CE Wash head 12 pins Wash head 8 pins Bracket Wash bottle Waste bottle Wash cap CODE DESCRIPTION 23000090 Waste cap assembly 12320090 Dispense pump assembly 12310180 Cleaning Needle 12320040 Motor 23000110 Solid valve 12310590 Vacuum pump 21000060A Power switch assembly 23000040 Power supply board 11900140 Fuse 3 15A 250V without wash cover 23000090 including warning assembly without waste cover 12310180 Including 2 long short needles without wash tube 23000040 Power board PCBA with case Including power switch and fuse 28 Wash8to12 User s Manual 7 PICTURES OF SOME SPARE PARTS Here are some pictures of the spare parts of Wash8to12 in common use 8 pins Wash head Wash solution tube Wash head tubes Waste bottle tubes Dispense pump 29
18. those specified Verify the front covers closed while the instrument in operation Avoid touching the mechanism such as the 2 axis motion platform inside the instrument while the instrument is operating This may cause serious personal injury or damage the instrument Wash8to12 User s Manual 1 GENERAL INFORMATION 1 1 How to use this manual This User s Manual contains descriptions and instructions for the operation and general maintenance of the Wash8to12 Microplate Washer To ensure optimum performance of the instruments these procedures should be followed as stated This manual gives you all the information needed to Install the Wash8to12 Microplate Washer Understand the Wash8to12 Set the Wash8to12 system up to meet your exact needs Run wash protocols using the Wash8to12 system Wash8to12 User s Manual 1 2 Copyright BGT BioGenTechnologies GmbHGT Von Langen Weg 10 D 48565 Steinfurt Tel 02551 4090 Fax 02551 1298 Web www Biogentechnologies de E Mail info biogentechnologies de BGT BioGentechnologies GmbH Hereinafter called BGT owns all rights to this unpublished work and intends to maintain this work as confidential BGT may also seek to maintain this work as an unpublished copyright This publication is to be used solely for the purposes of reference operation maintenance or repair of BGT equipment No part of this can be disseminated for other purposes In the event of inadvertent or deliberate
19. ually the position of the liquid suction needle is adjusted to the position that is aligned with the top of the Microplate well Press the START key to save the changing or the CANCEL key to ignore them 24 Wash to12 User s Manual Please watch the position of the wash head while you are adjusting the Top Aspiration position Make sure that the wash head has been adjusted to a little above the cuvette as shown in Figure 7 Figure 7 Adjust Top Aspiration position 4 2 7 Adjusting Pump The fine tuning of the diving pump s voltage can slightly adjust the speed of washing liquid injection The value 0000 is recommended Adjusting Pump By pressing the or the key the adjustment value is increased or decreased in steps of 1 The adjustment range is between 0 and 10 Press the START key to save the changing or the CANCEL key to ignore them 25 Wash8to12 User s Manual 5 MAINTENANCE AND CLEANING 5 1 Daily maintenance of worktable 5 2 There is often remnant liquid on the worktable and excessive liquid may spill over the edge of the worktable and seep into the instrument Therefore it is necessary to wipe off remnant liquid on the worktable from time to time Use tweezers to nip a small piece of absorbent cotton to wipe the worktable Use caution that the head of the tweezers don t scratch the surface of the worktable Daily maintenance of dispensing head The dispensing head is one of the core com
20. ust be equipped with glove to touch waste bottle at time of disposing of waste liquid Pay attention to guard against the bottle waste liquid leakage Chemical The operator is responsible for taking all necessary precautions against hazards associated with the use of clinical laboratory chemicals Wipe up any reagent spillage on the instrument immediately Biohazardous Materials As sample container Microplate should be treated as the potentially biohasardous All materials Wash to12 User s Manual and mechanical components associated with the sampling and waste system should be handled according to your facility s biohazard procedure Use the personal protective equipment recommended by your facility when handling any of these components Detailed recommendations Waste solutions and solid wastes Avoid direct contact with waste solution and or solid waste Both should be handled as potentially biohazardous Dispose of waste solution and or solid waste according to the relevant governmental regulations ADDITIONAL PRECAUTIONS Flammables Avoid using dangerous flammable material around the instrument Accuracy Precision of the Measured Results For proper use of the instrument measure control samples and monitor the instrument during the operation Operation and Maintenance During operation and maintenance of the instrument proceed according to the instructors and do not touch any parts of the instrument other than
21. wash bottle cover Thereafter put the pump into the wash bottle Figure 4 Wash bottle with pump Fill the wash bottle with the wash solution Place the wash bottle behind the instrument Connect the wash tube to the wash bottle tube connector at the rear panel of the 14 Wash8to12 User s Manual instrument see figure 2 Connect the dispense pump power cable to corresponding connector at the rear panel of the instrument see figure 2 3 3 Installing the Waste Bottle Place the waste bottle behind the instrument Connect the waste tubes to the waste bottle tube connectors at the rear panel of the instrument see figure 2 Connect the level sensor cable to corresponding connector at the rear panel of the instrument see figure 2 3 4 Installing the dispensing head Twist one end of the dispensing tube white connector and the discharge tube transparent connector onto the tubular joints on the worktable according to their colors Twist the two tubes onto the same colored tubular joints of the dispensing head Put the dispensing head from above onto the supporting arm Make sure that both ends of the steel pin of the dispensing head are placed stably in the fillister of the supporting arm f necessary adjust the dispensing head parallel to the worktable with the two adjusting screws at the root of the supporting arm 3 5 Turn on the system Fill up the wash bottle with washing liquid Tak
22. within the range of 1mm in both vertical and horizontal direction so as to adapt the to microplates of different specifications In the horizontal direction the liquid suction needle the thick long needle is required to be put at the center of the Microplate well In vertical direction the liquid suction needle is required to fall completely onto the bottom of the Microplate well A clearance of 0 5 to 1mm is recommended between the steel pin of the dispensing head and the fillister of the supporting arm groove Adjusting Wash Head Horizontal 0 1mm Vertical 0 1mm 23 Wash8to12 User s Manual By pressing the SELECT key you can change between horizontal and vertical adjustment of the wash head By pressing the or the key the adjustment value is increased or decreased in steps of 0 1mm Press the START key to save the changing or the CANCEL key to ignore them 4 2 5 Adjust the horizontal of the wash head Adjust these keys to make the wash head horizontal Figure 6 Adjust the wash head 4 2 6 Adjusting Top Aspiration When the amount of dispensation is higher than the capacity of the Microplate wells the system will automatically initiate the top aspiration If the top aspiration doesn t function properly the position of top aspiration should be adjusted Adjusting Top Aspr By pressing the or the key the adjustment value is increased or decreased in steps of 0 1mm Us

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