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Gilson 215 User's Manual

1. C7 SPUBWIWWOD DOISD GSIOC Commands Appendix Smm Ta b c d C 8 Reads the XYZ travel range Returns axis min max where axis X Y orZ min Lowest position in tenths of millimeters max Highest position in tenths of millimeters The first time the command is sent it returns the X range the second time it returns the Y range the third time it returns the Z range Reads front panel LED display Returns cccccccc which are the eight characters currently shown on the display If a character is non printable the hexadecimal ASCII code for the character is returned Reads the command in the synchronization buffer There may be up to eight commands pending Returns if buffer is empty Sends a synchronized buffered command mm that will be executed when the instrument is quiescent Sending a command can overwrite unexecuted existing commands If you send this command without indicating a parameter mm the buffer is cleared Sets the Z height from the locator plate up in tenths of millimeters for each probe where ais the Z height for probe A b is the Z height for probe B c is the Z height for probe C d is the Z height for probe D If a Z height is not specified that probe will not move For example T500 300 sets the probe A Z height to 500 and probe C Z height to 300 Reads the last set Z height using the buffered T command from the locator plate up in tenths of mi
2. EN 61326 EN 1000 3 2 1995 EN 61000 3 3 1995 FCC Part 15 91 4 x 61 0 x 60 8 cm 36 0 x 24 0 x 24 0 in Maximum height Z arm height is adjustable to accommodate vessel heights between 1 and 150 mm Frequency 50 to 60 Hz Voltage 100 120 V or 220 240V mains voltage fluctuations not to exceed 10 of the nominal voltage Current rating 25A for 100 120 or L4A for 220 240V Accuracy 0 5 mm in X Y dimensions 1 mm in dimension Repeatability 0 25 mm in X 2 dimension Probe rinsing occurs through a dedicated rinse station for rinsing the inside and outside of the probe selectable rinse volume and flow rate XYZ with stationary rack design Software control Computer control via RS 232 or GSIOC and Gilson 735 Sampler Software Syringe pump External integral high precision four piston syringe pump 444 Quad Dilutor Vertical punch strength 3 06 kg 6 74 Ibs per probe Installation 2 This section takes you through the steps for setting up your Quad Z 215 Liquid Handler cabinet 849 Multiple Y arm rack s locator Injection Module plate 2 1 Locator Plate Installation Installation 2 2 Locator Plate Installation The locator plate serves two functions e Positions the racks and accessories that fit onto the bed of the liquid handler e Contains liquid spills such as those caused by overflowing vessels The locator plate and its four mounting screws are shipped i
3. Probe Installation There are different probes available for use on the Quad Z 215 Liquid Handler Depending upon your application you have purchased the appropriate probes and probe guide inserts When installing the probes or custom sized probe guide inserts 1 3 or 1 5 mm refer to the following procedures and diagrams Installing the Probe Guide Inserts Your Quad Z is delivered with 1 5 mm probe guide inserts installed If you ordered custom sized probe guide inserts for more precise XY accuracy refer to the installation instruction bdow To install the probe guide inserts turn the insert dockwise into the probe guide Installing the Probes Insert the probes into the top of the isolation probe holders and pull them through the holders and the probe guide inserts until the tip of the probe is in the probe guides Installation Plumbing Connections Transfer Tubing Installation il Connect one end of each piece of transfer tubing to the 444 QuadDilutor Refer to the 444 QuadDilutor User s Guide for more information Locate the level sensing cables LLD cables supplied with the Z arm Pass the other end of each transfer tubing through the open end of an LLD cable casing nearest the right angle connector The tubing should exit the back of the LLD cable casing approximately 6 5 cm from the end Connect each tubing to the top of the isolation probe holder using a 1 4 28 nut and ferrule supplied with the
4. 215 Liquid Handler 215QA DvX XX Operation 3 Pump Type Because the Quad Z 215 Liquid Handler uses an external dilutor Blank Front is selected and all options are inactive Syringe Options All options on this tab are inactive for the Quad Z 215 Liquid Handler because it has an external dilutor 3 7 Jajpuey pinbiq y Bunn uo Configuring the Liquid Handler Operation 3 8 Rinse Site The instrument stores the location of a rinse site in memory This allows the instrument to move to this location before homing the syringe and prevents the spilling of waste liquid or rinse diluent You must modify the rinse site coordinates if you have a Quad Z 215 Liquid Handler Refer to the following table to select the correct X Y and Z coordinates depending on the kind of probe rinse that will be performed Coordinates for the rinse site are provided for the X Y and Z axis in millimeters The coordinates identify the rinse site for the left most probe installed on the Quad Z 215 Liquid Handler X Y Z Shallow pocket rinse 82 9 16 5 100 0 Deep pocket rinse 82 9 3 8 47 5 Flow through rinse 82 9 3 8 47 5 Operation 3 Liquid Detector The Liquid Detector tab allows you to adjust the default sensitivity for liquid detection Note Gilson control software such as 735 Sampler Software will override these settings Raising the percentage suppresses false liquid detection while lowering the percentage inc
5. Replacing Tubing Replacing a Probe Replacing a Level Sensing Cable Replacing a Fuse Checking Position Alignment Transporting the Liquid Handler 5 Troubleshooting Error Messages M echani cal Electrical Liquid Level Detector Repair and Return Policies Appendix A Replacement Parts and Accessories Control Software Probe Guide Inserts Transfer Tubing and Waste Bottle Rinse Station Rack Accessories Cables and O Accessories Miscellaneous Appendix B Racks Peltier Racks Appendix C GSIOC Commands GSIOC Commands GSIOC Command List Appendix D Pipetting Techniques Minimizing Cross Contamination Minimizing Dilution Effect Declaration of Conformity Application of Council Directives 89 336 EEC 73 23 EEC Standards to which Conformity is Declared EN 61326 EN 61000 3 3 EN 61000 3 2 EN 61010 1 Manufacturer s Name Gilson Inc Manufacturer s Address 3000 W Beltline Highway Middleton WI 53562 EC Office Address Gilson S A S 19 Avenue des Entrepreneurs B P 145 F 95400 Villiers le Bel France Type of Equipment Laboratory Equipment Moto Ke eas Ae sate eye ier eer E E cae ere Quad Z 215 Liquid Handler Beginning with Serial Number 250A 1K 001 Month and Year of Manufacture January 2001 the undersigned hereby declare that the equipment specified above conforms to the above Directives and Standards pdt Place Middleton WI USA Michael Jacquart Issue Date January 2001 Senior Vice Presid
6. point The default XY coordinates are shown next to the model number Operation model 819 X coordinate 544 0 mm Y coordinate 3 8 mm If the model 819 is not installed next to the right support use the following coordinates X coordinate 351 1 mm Y coordinate 1 3 mm model 841 X coordinate 475 3 mm Y coordinate 3 8 mm model 889 X coordinate 336 1 mm Y coordinate 3 8 mm model 849 X coordinate 336 1 mm Y coordinate 3 8 mm other 3 11 Ja puey pinb7 y Buun uos Configuring the Liquid Handler Operation 3 12 Z Height Use the Z Heights tab to identify the size of the installed Z arm and the height at which the Z arm is clamped The tower height options are inactive for the Quad Z 215 Liquid Handler because there is only one tower height available The Z arm can be damped at an adjustable height over the locator plate You can set this height so that the liquid handler is able to properly find heights that you specify Type the damp height in millimeters A damp height of 0 mm means the Z arm is flat on the locator plate Operation 3 Home Phase F HS etep Liity Use the Home Phase tab to display the current X and Y phase of the instrument Clicking Start causes the liquid handler to perform the phase procedure This procedure consists of the liquid handler homing itself 10 times The liquid handler finds out where home is located by feeling for the back and left walls
7. the horizontal arm Always lift the instrument from its base 13 BHuryoedun Unpacking Introduction Standard Equipment Once the liquid handler and the accessories containers have been unpacked you should have the following Quad Z 215 Liquid Handler 444 QuadDilutor with accessories Locator plate with one drain base includes four mounting screws Rinse drain package which includes e 2 liter waste bottle e Cap with quick connect fitting e Rinse station with fittings e 5 feet of Tygon waste tubing with quick connect fitting Z arm and control cable with retaining clip and level sensing cables Accessory package which includes Fuse drawers fuses and power cords 10 pin terminal block connector 8 pin terminal block connector 9 64 ball driver for removal of armlock Eight tubing retaining clips Cable support rod with bracket and two Phillips head attachment screws e Tubing support rod 215 Utility Programs CD ROM 444 Utility Programs CD ROM Quad Z 215 Liquid Handler User s Guide 444 QuadDilutor User s Guide Accessories Based upon your configuration you ll also receive additional accessories such as the probes transfer tubing racks etc If necessary refer to Appendix A for part numbers Introduction Customer Service Gilson Inc and its worldwide network of authorized representatives provide customers with the following assistance sales technical applications and instrument re
8. GSIOC cable to link an additional Gilson GSIOC module to the liquid handler and control both devices via a program executed on the computer pin tomalo 4 i Connector connect LI yy a A jH to quid handier Connect the female connector located individually at one end of the cable into the GSIOC port of the liquid handler Tighten the retaining screws Refer to diagram below Connect the other female connector located on the same end as the male connector to the Gilson module Tighten the retaining screws If you re connecting another Gilson module use the male connector to join another GSIOC cable and make the necessary connection to the next Gilson module Renae Liquid Handia mI Gisan devicn peg esot cable cc 2 20 Installation 2 Unit ID and Baud Rate Mode Selection Use the SW1 selector to choose a different unit ID and the SW2 to choose a different baud rate mode If necessary refer to the diagram on page 2 16 for the location of these selectors Unit ID The unit ID identifies the liquid handler to Gilson software packages that can issue GSIOC commands to the liquid handler At the factory Gilson set the unit ID to 22 There is no need to change this number unless it is the same as that assigned to another Gilson device that s also connected along the GSIOC To change the unit ID 1 Gently insert a small flat blade screwdriver into the SW1 selec
9. b Z motor on probe B c Z motor on probe C d Z motor on probe D w pitch motor For each status you ll see U for unpowered P for powered R for running or E for error Start to move probes to the Z height positions Set using the buffered T command see page C 8 Start to move probes to the Z height positions set using the buffered T command see page C 8 with liquid level sensing on Appendix Oabcd B C Reads the liquid detector output Returns a b c d where a leftmost probe probe A b probe B c probe C d probe D For each status you ll see A for air or L for liquid Sets each probe speed value in um s where a speed of probe A b speed of probe B c speed of probe C d speed of probe D If the value is not specified the probe speed value will remain the same The default speed is 125000 um s Reads each probe speed value in um s Returns a b c d where a speed of probe A b speed of probe B c speed of probe C d speed of probe D Reads X and Y encoder positions in integral units of 0 1 mm Reads probe range values in the X direction for each probe a d in sequence where a minimum X value maximum X value b minimum X value maximum X value c minimum X value maximum X value d minimum X value maximum X value The range is displayed in integral units of 0 1 mm The values are based on the current probe pitch Changing the pitch will alter the ranges
10. of the unit The liquid handler expects to find these walls in the same place each time If it does not you will get an error If this error was caused by an obstruction just clear the obstruction and try again If the problem does not clear or if a change is made to the mechanics you will probably need to repeat this option to find the true home location Once the process completes the spreadsheet displays the values generated from each phase procedure 3 13 Ja puey pinbi7 y Buun uos Configuring the Liquid Handler Operation 3 Safety Contact The instrument has provisions for connecting safety devices that your application may require as long as they present a contact closure or TTL type interface The Safety Contact tab allows you to specify which input contact is connected to the safety device and what is the active state of that device The function of the safety contact is equivalent to pressing the Stop button on the instrument s front panel Bearing Life The Bearing Life tab displays the XYZ travel in kilometers 3 14 Operation 3 Sound and Display Use the options in the Sound and Display tab to adjust the brightness of the display sound level and tone Clicking Beep tests the sound level and tone that are currently selected For the L E D brightness you can select a range of 0 through 7 where 0 is the dimmest and 7 is the brightest Default setting is
11. tubing Firmly tighten this fitting using the supplied headless nut extender part number 49041032 since it holds the probe in place 2 7 SUOI D9UUOD Huiquinid Plumbing Connections Installation __ 2 Installing the Level Sensing Cables To install the level sensing cable 1 Plug the right angle connector on one end of the cable into socke one on the back of the Z arm 2 Snap the cable into the LLD cable guide refer to the picture above 3 Loosen the small hexagonal nut on the probe holder for probe one Attach the split tongue connector to the nut Retighten the nut until snug approximately one turn after finger tightening 4 Repeat for probes 2 through 4 2 8 Installation 2 Rinse Station and Drain Waste Tubing Installation You ll clean the probe using the rinse station To eliminate carryover of liquids the rinsing procedure pumps an excess volume of diluent or probe washing solution through the probe and out into the rinse station The small diameter of the rinse station inserts allow the outside of the probe to be washed along with the inside The rinse station s design accommodates three kinds of rinses e Shallow pocket rinse Used for level sensing applications where the probe is only immersed in a few top hole use the wi top hole in the millimeters of the sample dres iin for storage of the H unused barbed e Deep pocket rinse Used fing or plac for non level sensing applica
12. 00 120V accepts one fuse The fuse drawer for 220 240V accepts two fuses 4 Insert the fuse drawer into its receptacle in the liquid handler See rear panel diagram on page 2 16 Fuse installation for 100 120 voltage Fuse installation for 220 240 voltage 2 23 SUOI OBUUOD e Electrical Connections Installation 2 24 Power Cord Connection Locate the appropriate power cord for your line voltage Discard the other power cord Use the power cord to connect the liquid handler to an AC power source Operation 3 The Quad Z 215 Liquid Handler is controlled as follows e Via a method running on a personal computer You create the method using 735 Sampler Software ordered separately part number 2106773532 If you have not already done so install the 735 Sampler Software Refer to the documentation supplied with the software 3 1 Front Panel Operation ower indicator light 3 2 Start Stop T LED display Front Panel The front pane of the liquid handler contains a Start button Stop button LED display and power indicator light Start Button The Start button can be used to home the XY Z arm when the liquid handler is first powered up or when the motors for the XYZ arm have been relaxed When pressed the yellow LED lights Stop Button The Stop button is a large touch sensitive pad that can be used to terminate a program and stop the liquid handler from respond
13. 26 27 28 5 2 Description NV RAM checksum is invalid X scale factor is invalid Y scale factor is invalid X motor position error Y motor position error Z motor position error X target less than minimum X X target more than maximum X Y target less than minimum Y Y target more than maximum Y Z target less than minimum Z Solution e Send the buffered 9 GSIOC command to reset the NV RAM and initialize to the default value Run 215SETUP EXE or the 215 Setup Utility program e Replace the main board Contact the Gilson Customer Service Department Contact the Gilson Customer Service Department Turn power off then on to the liquid handler Turn power off then on to the liquid handler Turn power off then on to the liquid handler Send the immediate Q command using the GSIOC Utility Program to read the travel range Correct the error in the program controlling the liquid handler Send the immediate Q command using the GSIOC Utility Program to read the travel range Correct the error in the program controlling the liquid handler Send the immediate Q command using the GSIOC Utility Program to read the travel range Correct the error in the program controlling the liquid handler Send the immediate Q command using the GSIOC Utility Program to read the travel range Correct the error in the program controlling the liquid handler Send the immediate Q command using the GSIOC Utility Program to read the travel
14. 5 Emergency Contact The emergency stop option provides for sending a signal to a peripheral device such as a Gilson 818 AutoMix whenever the liquid handler s Stop button is pressed or safety input is activated Note Once an emergency output has been activated reset the contact to its non emergency state using the 215 Contact Test Utility 3 15 Jajpuey pinbiq y Bunn uo Testing the Liquid Handler s Contacts Operation 3 16 Testing the Liquid Handler s Contacts Following is a description of how to use the 215 Contact Test Utility to toggle output contacts to determine if the correct contact connections have been made to peripheral devices to be controlled by the Quad Z 215 Liquid Handler The program also identifies the state of input contacts and lets you test the Start and Stop buttons on the liquid handler Before using this software you need to connect the peripheral device s inputs to the appropriate output pair on the liquid handler If necessary refer to Section 2 Installation for information on making contact connections Maintenance 4 To obtain optimum performance and maximum life from the Quad Z 215 Liquid Handler it is important to keep the instrument wd l maintained This section contains some general guidelines that will help you to maintain your liquid handler 4 1 Helpful Hints Maintenance 4 2 Helpful Hints In order to keep your liquid handler at peak performance G
15. 9550 and 832 Temperature Regulator part number 2759502 B 3 syory Racks Appendix B 4 Code 32 rack Thermostated rack for 60 vessels Material aluminum Vessels and maximum capacity 13 x 65 mm tubes 6 mL 13 x 100 mm tubes 9 mL Part number 2704432 Required accessories adapter plate part number 2504621 and thermostating cuvette part number 2704429 or thermostating cuvette part number 2759550 and 832 Temperature Regulator part number 2759502 Code 33 rack Thermostated rack for 14 scintillation vials Material aluminum Vessels and maximum capacity 28 x 60 mm 20 mL Part number 2704433 Required accessories adapter plate part number 2504621 and thermostating cuvette part number 2704429 or thermostating cuvette part number 2759550 and 832 Temperature Regulator part number 2759502 Code 200 rack For 96 vessels Material aluminum Vessels and maximum capacity 13 x 100 mm 9 mL Part number 2504600 Appendix B Code 201 rack microplate tube with stopper Eda Eppendorf re vial with For two microplates two Eppendorf vials and asap top two 13 x 100 mm tubes TS Material aluminum 4 Vessels and maximum capacity 96 well microplates Eppendorf vials 15 mL 13 x 100 mm tubes 9 mL Part number 2504601 Code 201H rack For two microplates two Eppendorf vials and two 13 x 100 mm tubes with microplate covers Material aluminum Vessels and maximum
16. Dimensions 269 x 1 3 x 0 8 mm ID Requires probe guide insert part number 25064471 for precise XY accuracy Micro septum piercing probe constricted 45 bevel tip capacitive level sensing stainless steel Dimensions 269 x 1 5 x 1 1 mm ID tip dimensions 10 x 0 7 x 0 4 mm ID Requires probe guide insert part number 25064472 for precise XY accuracy Non septum piercing probe constricted beveled tip capacitive level sensing stainless steel Dimensions 269 x 1 5 x 0 8 mm ID tip dimensions 15 x 0 9 x 0 45 mm ID Requires probe guide insert part number 25004472 for precise XY accuracy A 1 Replacement Parts and Accessories Appendix 2507255 Control Software 2106773532 Probe Guide Inserts 25064473 25064472 25064471 25064475 A Non septum piercing probe beveled tip capacitive level sensing stainless steel Dimensions 269 x 1 5 x 0 4 mm ID Requires probe guide insert part number 25064472 for precise XY accuracy 735 Sampler Software running directly from PC requires PC with Microsoft Windows NT and serial cable Probe guide insert for 1 8 mm outer diameter probes Probe guide insert for 1 5 mm outer diameter probes Probe guide insert for 1 3 mm outer diameter probes Probe guide insert for 2 7 mm outer diameter probes Transfer Tubing and Waste Bottle 250531734 250531744 250531754 49041034 49041050 49041035 49041022 A 2 11 mL 1 6 mm 1 16 OD transfer tub
17. Order Peltier Controller part number 2515331 separately wut Ny syory Racks Appendix B 12 Code 852 Peltier rack For two Becton Dickinson Falcon 96 well shallow well assay plates U bottom style Material aluminum Part number 2514852 Note Order Programmable Peltier Controller part number 2515850 separately Code 853 Peltier rack For 96 12x32 mm 2 ml flat bottom vials Material aluminum Part number 2514853 Note Order Programmable Peltier Controller part number 2515850 separately Code 854 Peltier rack For two Ritter or Beckman 96 wdll deep well microplates Material aluminum Part number 2514854 Note Order Programmable Peltier Controller part number 2515850 separately GSIOC Commands C The Gilson Serial Input Output Channel GSIOC is an asynchronous serial communications interface that enhances the power of your Gilson equipment The GSIOC incorporates an EIA RS 485 interface and allows up to 32 slave devices to be controlled from a single master in a multi drop configuration Each slave device is identified by a unique number which must be known to the device and to the computer The default ID code of the Quad Z 215 is 22 To control the Quad Z 215 Liquid Handler via the GSIOC interface you will need the following e apersonal computer with Microsoft Windows 98 or Windows NT and any Gilson control software or 706 Device Driver Software installed e an unused
18. Quad Z 215 Liquid Handler User s Guide LT2545 2003 Gilson Inc All rights reserved April 2003 Table of Contents 1 2 Declaration of Conformity Introduction Installation Safety Precautions Description Unpacking Standard Equipment Accessories Customer Service Technical Specifications Locator Plate Installation Armlock Removal Z Arm Installation Installing the Z Arm Cable Support Rod Probe Installation Installing the Probe Guide Inserts Installing the Probes Plumbing Connections Transfer Tubing Installation Installing the Level Sensing Cables Rinse Station and Drain Waste Tubing Installation 2 9 Rack Setup Code 200 Series and Code 500 Series Racks 2 11 Code 200 Series and Code 20 Series or Code 30 Series Racks Only Code 20 Series or Code 30 Series Racks Final Z Arm Adjustment Electrical Connections Rear Panel Input Output Ports RS 232 Port GSIOC Port Unit ID and Baud Rate Mode Selection Power Cord Connection 3 Operation Front Panel Start Button Stop Button LED Display Power Indicator Light Running Programs Configuring the Liquid Handler Pump Type Syringe Options Rinse Site Liquid Detector Adjust XY Home Phase Safety Contact Bearing Life Sound and Display Emergency Contact Testing the Liquid Handler s Contacts 4 Maintenance Helpful Hints Cleaning the Liquid Handler Cleaning the Fluid Path Lubricating Horizontal Pitch and Vertical Rods Replacing Parts
19. RS 232 communication port From the controller you e specify the Quad Z 215 as the device you want to control e issue commands that set operating parameters control operation or request information from that instrument C1 GSIOC Commands Appendix Command C2 Type l GSIOC Commands There are two kinds of commands that you can send over the GSIOC e Buffered commands send instructions to the liquid handler These commands are executed one at a time e Immediate commands request status information from the Quad Z 215 These commands are executed immediately temporarily interrupting other commands in progress GSIOC Command List In the command list on the following pages the GSIOC command must be entered in the proper upper or lower case format If a buffered command requires additional information you ll see italidzed text next to the command The description of the command identifies what you need to enter in place of the italicized parameter Also note that if a parameter is optional it appears within brackets Immediate B Buffered Description Identifies the selected slave device Returns character string 21I5QADVx yz where x y and z represent software version Resets the Quad Z 215 Returns and resets the instrument to its power up state which dears any error state relaxes all axes opens contact closure inputs Appendix aal xxxx C
20. Reads non volatile memory NV RAM at current address Returns aaa xxxx where aaa Value between 0 and 19 for word type data or 100 to 119 for floating type data xxxx Data at the address Current address is incremented Sets the value at NV RAM address where aa Value between 0 and 19 for word type data or 100 to 119 for floating type data xxxx Optional Data at the address Sets test mode depending on value assigned to n 1 XYZ test 9 Reset NV RAM and initialize to defaults Reads contact input event FIFO Returns Xtttttt where X State of the four contact inputs 1 for closed O for open See table below Time since the last buffered 9 command 3 D VOZZr A LTA MMIVAWLPE x PROrFOrROPRPOrFROrROFrFOROD gt D RPFrRrFOOFRrFOOFRFOOFRFrFOOW FPREPRPPOODCOORPRPHPRFPOOCOOOND PRPRPREPRPRPRPRrFPRrRFODOOCOOCOCO Whenever the status of a contact input changes the state of all contacts and the time since the last buffered 9 command in the event FIFO is encountered C3 spuewwo9 DOISD GSIOC Commands Appendix 9 B A Bf d B CV ss SS B e e B C4 C Clears the contact event FIFO and resets the contact input event timer r Reads home phases for X and Y motors Returns xxx yyy where Xxx X motor phase yyy Y motor phase Causes 215 to beep f Frequency in Hz default is 2400 d Duration of beep in tenths of seconds default is 1 Sets user definable characte
21. apacity 16 x 100 mm 12 mL Part number 2504607 Code 208 rack For 70 vessels Material aluminum Vessels and maximum capacity 18 x 150 mm 25 mL Part number 2504608 Code 209 rack For 96 vessels Material aluminum Vessels and maximum capacity 12 x 32 mm Part number 2504609 B 7 syory Racks Appendix B 8 Code 210 rack For 75 vessels Material aluminum Vessels and maximum capacity 16 x 75 mm 11 mL Part number 2504610 Code 211 rack For 9 Boston round screw cap bottles Material aluminum Vessels and maximum capacity 48 x 113 mm 125 mL Part number 2504611 Code 212 rack For 96 vessels Material aluminum Vessels and maximum capacity 48 13 x 100 mm 9 mL 48 13 x 75 mm 7 mL Part number 2504612 Note Place the shorter tubes into the notched tube locations Code 213 rack For 74 vessels Material aluminum Vessels and maximum capacity 37 16 x 100 mm 32 mL 37 16 x 75 mm 25 mL Part number 2504613 Note Place the shorter tubes into the notched tube locations Appendix Code 214 rack For 96 vessels Material aluminum Vessels and maximum capacity 48 10 25 x 47 mm 2 5 mL 48 10 25 x 64mm 44 mL Part number 2504614 N ote Place the shorter tubes into the notched tube locations Code 216 rack For 60 Waters WISP vials Material aluminum Vessels and maximum capacity 60 15 x 45 mm 4 mL Part number 2504616 Code 217 rack For 96 t
22. be used when an optional 849 Multiple Injection Module is installed A 3 S JJOSS 99Y pUe Sed jJuoWweoR doy Replacement Parts and Accessories Appendix Cables and I O Accessories 25061401 36083121 36083122 36083123 638308512 638310512 709910206 36078143 6730504007 Miscellaneous 2509211 4311403 A 4 Level sensing cable Serial cable IBM PS 2 type 25 to 25 pin Serial cable IBM AT type 9 pin female to 25 pin male Serial cable adapter 9 pin female to 25 pin male Terminal block connector 8 pin Terminal block connector 10 pin 2 conductor interconnect wire 6 for making contact connections Shielded GSIOC cable 30 5 0A T 5 0 Slo Blo fuse Armlock with hex screws 9 64 ball driver hex wrench for armlock Racks B The Quad Z 215 Liquid Handler can be configured with a variety of rack types and sizes The following pages describe the racks that can be purchased for use on the liquid handler Refer to Section 2 for rack installation procedures B 1 Racks Appendix B 2 Code 20 rack For 108 vessels Material polypropylene Vessels and maximum capacity 10 x 100 mm tubes 4 5 mL Part number 150425 Required accessories adapter plate part number 2504621 Code 21 rack For 60 vessels Material polypropylene Vessels and maximum capacity 13 x 100 mm tubes 9 mL Part number 150422 Required accessories adapter plate part number 2504621 Code 23 rack For 44 vessels Ma
23. ble work area 58 5 x 325 x 17 5 cm 23 0 x 12 8 x 6 9 in Wx D x H Arm speed Contact control 45 7 cm sec gt 18 in sec in X dimension 240 6 cm sec gt 16 in sec in Y dimension Four inputs contact closure TTL or open collector four relay outputs and one switched 24V DC 1A output Environmental conditions Indoor use Altitude up to 2000 m Temperature range 3 40 C Air pressure 75 105 kPa Pollution degree 1 or 2 in accordance with IEC 66 Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Front panel START soft key and emergency STOP soft key Horizontal motion strength A 5 kg 11 1 lbs Y 7 kg 15 6 Ibs Locator plate capacity Up to five Code 200 series racks in the standard locator plate Up to five combined Code 200 series and Code 20 30 series racks in the standard locator plate one adapter plate part number 2504621 is required for each Code 207 30 series rack Up to seven Code 20 30 series racks in the locator tray for Code 20 30 series racks part number 2504627 One Code 505 or 505H rack plus one Code 200 series rack suoleodyioads jed1uyoa Technical Specifications Introduction Manufacturing standards Physical space requirement Power requirements Probe positioning performance Safety certification UL3101 1 CSA C22 2 No 1010 1 92 EN 61010 1 EMC certification
24. capacity 96 well microplates Eppendorf vials 15 mL 13 x 100 mm tubes 9 mL Part number 2504601H Code 202 rack For 96 vessels SSeS SSESESOS Material aluminum IBSOSS0 9S OSS OSS Vessels and maximum capacity 10 25 x 47 mm 2 5 mL NSSSLSSLS OLS SS OS Part number 2504602 oS Code 203 rack For 96 vessels Material aluminum Vessels and maximum capacity 10 25 x 64 mm 4 mL Part number 2504603 B 5 J o Q Appendix B A Code 204 rack KE DNN DOOS For 27 scintillation tubes YSR OOO Material aluminum AY OROKO Vessels and maximum capacity 28 x 57 mm 20 mL DOSAO O AAA Part number 2504604 f has microplate tube with stopper Code 205 rack Eppendort Pe For two deep well microplates two Eppendorf vials and two 13 x 100 mm tubes i RY Material aluminum Ae Vessels and maximum capacity 96 deep well microplates o gt Eppendorf vials 1 5 mL 5 AA 13 x 100 mm tubes 9 mL hice Part number 2504605 Code 205H rack For two deep well microplates two Eppendorf vials and two 13 x 100 mm tubes with microplate covers Material aluminum Vessels and maximum capacity 96 deep well microplates Eppendorf vials 15 mL 13 x 100 mm tubes 9 mL Part number 2504605H B 6 Appendix Code 206 rack For 96 vessels Material aluminum Vessels and maximum capacity 13 x 75 mm 7 mL Part number 2504606 Code 207 rack For 75 vessels Material aluminum Vessels and maximum c
25. ciently if you have the following information e the serial number and model number of the equipment involved The serial number is visible on the back of the control panel of the liquid handler e the installation procedure you used e list of concise symptoms e list of operating procedures and conditions you were using when the problem arose e list of other devices connected to the liquid handler and a description of those connections e list of other electrical connections in the room Warranty repair Units covered under warranty will be repaired and returned to you at no charge If you have any questions about applicability please contact Gilson or your authorized representative Non warranty repair For out of warranty repairs contact your local Gilson representative or the Gilson Customer Service Department A Customer Service representative will discuss service options with you and can assist in making arrangements to return the equipment if necessary Troubleshooting Rebuilt exchange For some units rebuilt exchange components are available Contact Gilson for details Return procedure In the United States contact the Gilson Customer Service Department to obtain authorization before returning any Gilson equipment To return a piece of equipment Carefully pack the unit to prevent damage in transit Check with Gilson regarding proper method of shipment No responsibility is assumed by Gilson for damage cau
26. eed to vary your cleaning methods accordingly Use the following cleaning protocols as references and make any changes to them as required for the samples and reagents being pumped for your application Proteins and peptides Follow this procedure if the fluid path is in contact with proteins and peptides 1 Prime the fluid path using a weak detergent solution 2 Pause the priming sequence 3 After 30 minutes resume priming the fluid path using distilled or deionized water to pump the remaining detergent from the tubing into a waste container Prime the fluid path a minimum of 10 cycles with distilled or deionized water 4 When you re satisfied that the entire fluid path has been flushed with water end the priming sequence Maintenance 4 Acidic and basic compounds Follow this procedure if the fluid path is in contact with acidic and basic compounds 1 Prime the fluid path using a 0 1N NaOH solution Pause the priming sequence After 10 minutes resume priming the fluid path using distilled or deionized water Prime until the fluid path has been flushed with water Pause the priming sequence Prime the fluid path using a 0 1N NaOH solution Continue to prime until the fluid path has been flushed with 0 IN NaOH Pause the priming sequence After 10 minutes resume priming the fluid path using distilled or deionized water Prime until the fluid path has been flushed with water When you re satisfied that the en
27. ent Corporate Technology Development Introduction 1 Safety Precautions For safe and correct use of this instrument it is recommended that both operating and service personne follow the instructions contained in this guide when installing cleaning and maintaining this instrument Because the probes installed on the Z arm may contain a dangerous substance use the safety shield included with the instrument and do not interfere in the work area of the instrument until the liquid handler has completed its procedures If dangerous liquids are used adequate protection such as proper ventilation safety glasses etc should be used Always switch the power to off when making adjustments to the liquid handler The potential exists for bodily harm if you interfere with the work area of the instrument while it is running 1 1 Description Introduction Description The Gilson Quad Z 215 Liquid Handler is an XYZ robot that can automate any number of manual liquid handling procedures The Quad Z has four independently operated probes with variable horizontal spacing from 9 to 18 mm allowing access to virtually any tube vial or microplate configuration The optional 849 Multiple Injection Module can be configured with four sample loops of varying capacities to provide complete flexibility for open access laboratories The advanced liquid level detection for each independently operated probe minimizes carryover ensuring accura
28. er to the appropriate instructions bdow depending on whether you re replacing a probe with one of the same type or one of a different type Installing same type of probe To install a replacement probe of the same type that s currently installed 1 Remove the transfer tubing s 1 4 28 fitting connected to the top of the isolation probe holder 2 Grasp the current probe and push it up through the top of the isolation probe holder 3 Insert the probe into the top of the isolation probe holder and pull it through the holder and the probe guide insert until the tip of the probe is in the probe guide 4 Replace and tighten the 1 4 28 fitting 49 sued Buloejdey Replacing Parts Maintenance 4 10 Installing different type of probe To install a replacement probe of a different type than is currently installed you may want to obtain a new probe guide insert for precise XY probe accuracy 1 4 5 Remove the transfer tubing s 1 4 28 fitting connected to the top of the isolation probe holder Grasp the current probe and push it up through the top of the isolation probe holder Remove the current probe guide insert by turning it counterclockwise Then place the new probe guide insert into probe guide and secure it by turning it clockwise probe probe guide inseri Insert the new probe into the top of the isolation probe holder and pull it through the holder and the new probe guide in
29. et one on the back of the Z arm Pass the cable through the LLD cable guide Attach the split tongue connector to the small hexagonal nut on the probe holder Tighten the nut until snug approximately one turn after finger tightening Maintenance Replacing a Fuse A blown fuse may indicate the existence of another problem in the instrument If the replacement fuses blow don t try others Contact your local representative or Gilson See Before calling us on page 5 8 To change a fuse follow these steps 1 Disconnect the power cord from the power outlet and from the rear pane receptacle Locate the fuse drawer on the rear panel See page 2 16 if necessary Insert a small screwdriver into the notch next to the fuse drawer Twist the screwdriver to open and renove the fuse drawer The fuse drawer contains one 5 0A T Slo Blo fuse 5 x 20 mm size for a 100 120 voltage selection It contains two 5 0A fuses for a 220 240 voltage selection Remove the old fuse s and insert the new fuse s Insert the fuse drawer into its receptacle in the liquid handler Fuse drawer for 100 120 voltage selection Fuse drawer for 220 240 voltage selection 4 13 sued Buloejdey Checking Position Alignment Maintenance 4 14 Checking Position Alignment The 215 Setup Utility Adjust XY tab described in Section 3 allows you to test whether the liquid handler is properly aligned and t
30. id handler 215 Contact Test Utility Enables you to test contact connections Operation 3 Configuring the Liquid Handler The liquid handler comes from the factory with its configuration set by Gilson Configuration information is stored in the non volatile memory of the liquid handler Prior to using the liquid handler for the first time it is important to review and adjust the default configuration to make sure it is correct for your application The following pages describe how to use 215 Setup Utility to configure the liquid handler This information is also available in the on line help supplied with the program When you execute the 215 Setup Utility from the computer the following tabs appear about pump type syringe options rinse site liquid detector adjust XY Z height home phase safety contact bearing life sound amp display emergency contact Following is a description of each of the tabs 3 5 Ja puey pinbi7 y Buun uos Configuring the Liquid Handler Operation 3 6 The About tab allows you to indicate the unit ID of the instrument being configured so the 215 Setup Utility can communicate with that instrument This tab also lists software version and copyright information for the 215 Setup Utility Unless the Non Volatile RAM has been cleared information about the type of 215 you are using will appear automatically You will see the following indicating that you are using a Quad Z
31. ilson recommends that you do the following Change or clean the tubing regularly to maintain maximum performance Flush the probe housings and rinse stations daily with distilled or deionized water On a weekly basis flush with a 10 solution of bleach or weak detergent Check periodically to ensure that all fittings are tight Wipe up all spills immediately Cold fluids may cause leakage warm fluids to room temperature before running then through the system Lubricate the rods on the Z arm at least once every six months see page 4 7 Maintenance Cleaning Cleaning the Liquid Handler The liquid handler should be cleaned occasionally using a dry dean cloth Or if necessary use a cloth dipped in soapy water If liquid is accidentally spilled on the liquid handler wipe the instrument using a dry clean cloth Cleaning the Fluid Path Depending on your use of the liquid handler it may be necessary to flush the entire fluid path It s important to clean the fluid path if you won t be using the liquid handler for a while or if you re using a solution with a high salt concentration for a probe wash or as a diluent Prime the system using distilled or deionized water Check the beaker during the priming sequence to ensure it always has liquid in it 43 Hulueajy Cleaning Maintenance 4 4 Cleaning methods Depending on the samples or reagents that come into contact with the fluid path you may n
32. ing assembly for four probe 100 mL 1 0 mL syringes 5 5 mL 3 0 mm 1 8 OD transfer tubing assembly for four probe 5 0 mL syringes 10 5 mL 3 0 mm 1 8 OD transfer tubing assembly for four probe 5 10 and 25 mL syringes Upchurch P 250 ferrule for 1 6 mm 1 16 OD tubing anti twist Upchurch P 350 ferrule for 3 0 mm 1 8 OD tubing anti twist Upchurch P 287 bushing for 1 6 mm OD tubing headless anti twist Upchurch P 387 bushing for 3 0 mm OD tubing headless anti twist Appendix 23077310 470343706 23077332 Rinse Station 25045525 25245512 23077333 Rack Accessories A Waste bottle 2 liter with lid and quick connect fitting Tygon tubing 5 16 ID x 7 16 OD for connection between rinse station and waste bottle per foot Quick connect fitting to connect Tygon tubing to waste bottle Multiple Probe Quad Z 215 rinse station connects to rinse station base Rinse station base attaches directly to 215 locator plate Y connector to connect two rinse stations to one waste bottle For part numbers for available racks refer to Appendix B To create your own Code 200 style rack order the blank rack kit part number 254461 and rivet gun part number 4391002 2504621 2504627 Adapter plate for installing Code 20 or 30 series rack on locator plate Locator tray for installing up to seven Code 20 or 30 series racks on the locator plate Note This locator tray cannot
33. ing to any more commands coming from the running program This button also relaxes the motors for the XY Z arm so that you can easily lift the probe and move the arm When pressed the yellow LED light is turned off In a situation where an emergency stop is required pressing the Stop button immediately stops the liquid handler The Stop button is designed to be sensitive enough that if you just brush it with your hand it activates LED Display The amp character LED display shows the current status of the liquid handler and any error codes as they are encountered Your program can also contain instructions for showing 8 amp character messages on the display when the program is run Refer to Section 5 Troubleshooting for a list of current error codes and required actions Power Indicator Light This indicator becomes lit when you turn on power to the liquid handler using the power switch located on the rear panel Refer to the rear panel diagram on page 2 16 if necessary Operation 3 Start Up To start the liquid handler 1 2 Make sure the liquid handler is connected to a power source Turn on the liquid handler using the power switch located on the rear panel Refer to rear pana diagram on page 2 16 if necessary The power indicator light on the front panel illuminates When power is turned on the liquid handler beeps and displays the current version of its installed firmware This message appears for about
34. it is interfaced Outputs are contact closures 5 5 Electrical Troubleshooting 5 6 Unit not operational e Make sure power is turned on e Check AC power cord connections e Try different AC outlet e Check fuses replace if necessary e Check all liquid handler connections and make sure that the unit is plugged in Unit blows fuses e Contact the Gilson Customer Service Department Troubleshooting 5 Liquid Level Detector Liquid level detector not detecting liquid level e Ensure that the level sensing cables are plugged in e Check sensitivity setting in the 215 Setup Utility see page 3 9 and lower the percentage e Check if liquid is detectable Liquid level detection works only if there is dectrical conductivity in your liquid Liquid level detecting will not work with most non polar liquids For intermediate polarity liquids and polar liquids check the sensitivity setting in the 215 Setup Utility e Call Gilson if this is caused by faulty circuitry Liquid level detector falsely detecting liquid level e Ensure that probes are installed correctly e Check sensitivity setting in the 215 Setup Utility see page 3 9 and raise the percentage e Call Gilson if this is caused by faulty circuitry 5 7 10 99 9q 19497 pinbiq Repair and Return Policies Troubleshooting 5 8 Repair and Return Policies Before calling us Gilson Customer Service personnel will be able to serve you more effi
35. llimeters for each probe Returns ais the Z height for probe A b is the Z height for probe B cis the Z height for probe C d is the Z height for probe D Appendix vcs S WCc cC wn C Sends a GSIOC2 command directly to the Quad Z where c the type of command s ss the command string You may send up to four GSIOC2 commands before the first command completes The response is placed into a response buffer one string deep for later reading Reads the response string buffer Returns if buffer is empty Writes character string cc cc to the LED display Characters outside the printing range are shown in hexadecimal ASCII code on the display User definable characters are available with codes of 80 to 8F Sets the width between the probes in integral units of 0 1 mm where n is a value from 90 to 180 Reads the probe width in integral units of 0 1 mm Indicates X motor status Returns one of the following U for unpowered P for powered R for running or E for error Reads probe X positions in integral units of 0 1 mm Returns aaaaa bbbbb ccccc ddddd where aaaaa is the X position of probe A bbbbb is the X position of probe B ccccc is the X position of probe C ddddd is the X position of probe D Indicates Y motor status Returns one of the following U for unpowered P for powered R for running or E for error Reads the Y position Indicates Z motor status Returns abcd where ais the status of pr
36. m Installation Follow these steps to install the Z arm 1 Using a Phillips screwdriver loosen the mounting screw on the Z arm mounting bracket located on the Y arm Turn counterclockwise to loosen mounting screw mounting bracket 2 Partially pull out the bracket Do not remove completely 3 Place the Z arm into the mounting bracket You will need to insert one side of the Z arm into place at a time 4 Tighten the screw on the mounting bracket until the Z arm is secure You ll adjust the Z arm to its proper height after rack and rinse station installation This adjustment is described on page 2 14 2 4 Installation Installing the Z Arm Cable Support Rod 1 Using the two Phillips screws attach the cable support rod bracket in the holes located in the rear of the X arm extrusion cable support rod whip style 2 Extend the arm to the extreme X and Y direction to ensure that the cable will have enough slack Plug the Z arm control cable into the back topside of the control cabinet The control cable should be tucked into the groove located in the top of the X arm extrusion The retaining clip that is already on the control cable should be snapped onto the top of the cable support rod Refer to diagram below Clip cable to support rod Tuck cable into extrusion 25 uo ejje su WY Z Probe Installation Installation 2 6 probe probe guide insert probe guide
37. n a separate box with the liquid handler s accessories To install the locator plate onto the instrument bed 1 Make sure the locator plate s rinsing station base is at the rear of the instrument The locator plate will only install in this orientation 2 Align the four corner holes of the locator plate with the four holes on the instrument bed and lower the plate onto the bed 3 Using a Phillips screwdriver secure the locator plate using the four mounting screws pins to secure rinse station base s holes to secure locator plate Installation Armlock Removal The armlock on the liquid handler secures the Y arm during shipment You must remove the armlock prior to installing the Z arm and operating the instrument If the armlock is not removed the liquid handler cannot move in the X direction This results in an error state during operation If you need to move the liquid handler always reinstall the armlock This safeguards against mechanical damage To remove the armlock 1 Remove the cardboard label in front of the armlock 2 Using the 9 64 ball driver loosen the tensioning screw that immobilizes the Y arm 3 Using the 9 64 ball driver remove the two remaining screws that hold the armlock in place 4 Remove the armlock and store it and the ball driver for future use A s securin Y arm tensioning ssi screw 23 JEAOWIOY YOO WY Z Arm Installation Installation 2 Z Ar
38. nnected 0 if disconnected p Status of keypad and auxiliary power 1 if connected 0 if disconnected Sets output contacts and 24V external power cccc Output contacts 1 2 3 and 4 1 to connect 0 to disconnect X for no change p Optional Keypad and auxiliary power 1 to connect 0 to disconnect X for no change Pulses an output contact C Number of the output contact 1 4 t Duration of the pulse in tenths of seconds default is 1 which is 100 ms C5 SPUBWIWWOD DOISD GSIOC Commands Appendix KSxXxXxX C6 Sets liquid level sensing sensitivity where s selected probe a b c or d xxx Value from 0 to 255 0 is the most sensitive 255 is the least sensitive If s is not specified the default is abcd Reads liquid level sensing sensitivity Returns aaa bbb ccc ddd where aaa sensitivity of probeA bbb sensitivity of probe B ccc sensitivity of probe C ddd sensitivity of probe D Possible sensitivity range is from 0 to 255 for each probe O is the most sensitive 255 is the least sensitive Reads X Y Z motor status and dilutor status Returns xyzp For each status you ll see U for unpowered P for powered R for running or E for error Note Because the Quad Z uses an external dilutor the dilutor status is always unpowered U Read X Y Z motor status and width of the probes Returns xyabcdw where x X motor y Y motor a Z motor on probe A
39. o make minor adjustments to the X axis and Y axis offsets if needed You may need to use the options under this tab if the probe is not accessing the injection port of the injection module installed or the vessels in the installed racks This tab is described below The X offset and Y offset text boxes display the current offsets stored in the instrument s memory To determine if the probe s need s to be adjusted in the X or Y direction select the model of the injection module that is on your Quad Z probably an 849 or select other for a user defined test point The default XY coordinates are shown next to the model number below model 819 X coordinate 544 0 mm Y coordinate 3 8 mm If the model 819 is not installed next to the right support use the following coordinates X coordinate 351 1 mm Y coordinate 1 3 mm model 841 X coordinate 475 3 mm Y coordinate 3 8 mm model 889 X coordinate 336 1 mm Y coordinate 3 8 mm model 849 X coordinate 336 1 mm Y coordinate 3 8 mm other Maintenance Transporting the Liquid Handler When moving the liquid handler to another location or when sending it back to the factory do not use the Y arm as a handle Reinstall the armlock see Section 2 and always lift the liquid handler from the base 4 15 Ja puepy pinbiq y Hulodsuely Troubleshooting Error Messages Troubleshooting Error Messages Error 15 16 17 20 21 22 24 25
40. obe A b is the status of probe B c is the status of probe C d is the status of probe D Returns one of the following U for unpowered P for powered R for running or E for error C 9 SPUBWIWWOD DOISD GSIOC Commands Appendix Xax y Xbx y Xcx y Xdx y Yyyyy Zsp zsp C 10 Reads probe Z positions in integral units of 0 1 mm Returns aaaa bbbb cccc dddd where aaaa is the Z position of probe A bbbb is the Z position of probe B cccc is the Z position of probe C dddd is the Z position of probe D Moves probe A to target x and y coordinates Moves probe B to target x and y coordinates Moves probe C to target x and y coordinates Moves probe D to target x and y coordinates Sets new Y position yyyy is the new position in integral units of 0 1 mm Indicates Z motor status Returns one of the following U for unpowered P for powered R for running or E for error Sets new Z position where s the selected probe s p the new position in integral units of 0 1 mm If s is not specified the default is abcd Sets new Z position where s the selected probe s p the new position in integral units of 0 1 mm unless the liquid detector stops it first If s is not specified the default is abcd Pipetting Techniques D By following these techniques you ll be able to maximize accuracy and precision and minimize carryover with your Quad Z 215 Liquid Handler Accuracy and precision of pipetting are de
41. ocation of the RS 232 port refer to the diagram on page 2 16 Be sure your computer is turned off before making any connections To connect your computer to the liquid handler you ll need an RS 232 cable Obtain a cable with D connectors that are appropriate for the liquid handler and your computer The liquid handler requires a 25 pin male D connector Refer to the back panel of your computer or its documentation to determine which type of D connector it requires RS 232 cables are available from Gilson and your local computer store Connecting an RS 232 cable Attach the male end of the RS 232 cable to the RS 232 port located on back pand of the Quad Z 215 Liquid Handler Tighten the retaining screws Attach the female end of the RS 232 cable to the computer s RS 232 serial communications port Do not mistake it for the female 25 pin parallel printer port Again tighten the retaining screws 2 19 SUOI OBUUOD e91 99 9 Electrical Connections Installation 2 GSIOC Port Gilson systems feature a two way communication interface between the computer and most Gilson modules Communication occurs along the Gilson Serial Input Output Channel GSIOC The liquid handler can convert the RS 232 signal levels used by computers to the RS 422 485 signal levels required by the GSIOC and vice versa See page 2 19 for information on making the RS 232 connection between the liquid handler and computer GSIOC cable Use the
42. of the rack align with the pins on the locator plate 2 11 dnjas yoey Rack Setup Installation 2 Code 200 Series and Code 20 Series or Code 30 Series Racks To use a combination of Code 200 series and Code 20 series or Code 30 series racks on the locator plate 1 Install each Code 200 series rack as described on page 2 11 2 For each Code 20 series or Code 30 series rack place an adapter plate onto the locator plate Fit the plate so that the slots on the adapter plate align with the pins on the locator plate 3 Place the Code 20 series or Code 30 series rack onto the adapter plate 20 or 30 series rack locator plate 2 12 Installation 2 Only Code 20 Series or Code 30 Series Racks If all your racks are Code 20 or Code 30 series racks and you do not have the optional 849 Multiple Injection Module installed follow the steps bdow 1 Install the locator tray part number 2504627 ordered separately onto the locator plate of the liquid handler e For Code 20 series racks the handles face the front e For Code 30 series racks the hose fittings should face the back 2 Position each rack onto the locator tray You can install up to seven racks using this tray locator tray locator plate 2 13 dnjas yoey Final Z Arm Adjustment Installation 2 14 Z height adjustment tool Final Z Arm Adjustment Follow these steps to adjust the Z arm to the proper height 1 Turn off powe
43. one second before the LED display returns to a blank state In order to determine what PROM version is installed in your liquid handler you may need to turn the unit off then on again and watch the display for the version number to appear After the liquid handler powers up press the Start button This initiates the homing sequence that allows the liquid handler to determine its mechanical reference positions The sequence takes approximately one minute to complete While the homing sequence progresses the LED display shows H oming When the sequence completes it blanks Note If the program being executed by the liquid handler doesn t include commands for homing the instrument perform step 3 before starting the program The utility programs supplied with the liquid handler home the instrument if necessary 3 3 dN Hes Running Programs Operation 3 4 Running Programs The liquid handler is controlled by programs executed from a personal computer Executable EXE programs can be run from a computer The computer is connected to the liquid handler via an RS 232 cable Refer to Section 2 for correct installation of the RS 232 cable between the liquid handler and the computer The following utility programs may be used with the Quad Z 215 Liquid Handler and are supplied on the 215 Utility Programs CD ROM supplied with your liquid handler 215 Setup Utility Specifies configuration parameters for the liqu
44. ons Installation 2 16 Electrical Connections Rear Panel Input Output I O ports Gilson Serial Input Output Channel GSIOC port Keypad port not used on the Quad Z RS 232 port Fuse drawer Power switch Power receptacle Unit ID selector Baud rate mode selector OONADUBWNE o o o gt eh Bebe eae caf i E fy f MA SB A Installation 2 Input Output Ports You can use the input and output contacts found on the rear panel of the liquid handler to control peripheral devices Refer to the diagram on page 2 16 for the location of the input output ports Contact inputs The input terminal block of the liquid handler has aight contacts All of the inputs are paired and each pair includes a GROUND reference The contact input pairs are labeled A B C and D A contact is connected if it has a short across the input or is held low by aTTL output or other device Never connect voltages higher than 5V DC to an input When using TTL signals be sure to match GROUND connections Contact outputs The output terminal block has 10 contacts Pins 1 and 2 supply a 24V DC output Do not use this output unless the receiving device can accept 24V power Pins 3 through 10 are paired isolated relay contact closures and are labeled 1 2 3 and 4 Items you ll need To make connections you ll need the following e 2conductor cable 22 30 gauge for each wire e wire insula
45. pair If you need assistance please contact your Gilson representative or if you are in the United States call the Gilson Customer Service Department at 800 445 7661 or 608 836 1551 You can also contact the Customer Service Department via its e mail address service gilson com Specific contact information can be found on the Gilson web site at www gilson com To help us serve you quickly and efficiently please refer to the Before calling us section on page 5 8 1 5 BdIMIOS JOWOISND Technical Specifications Introduction Technical Specifications Please be aware of the following before operating the liquid handler Warning Changes or modifications to the liquid handler not expressly approved by Gilson could void the factory authorized warranty The liquid handler has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC commercial environment The liquid handler generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of the liquid handler in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at the user s own expense Shielded cables must be used with the liquid handler to ensure compliance with the Class A FCC limits Introduction ec Swen Accessi
46. pendent on the specifications of the pump you re using and the geometry of the probe tip D 1 Pipetting Techniques Appendix D 2 D Minimizing Cross Contamination Cross contamination of samples is caused by sample carryover on the probe tip or on its inner walls Several methods are available to minimize cross contamination e Useof the liquid level sensing capability will reduce carryover by limiting immersion below the liquid surface e Cleansing the probe tip with a flowing rinse removes contaminants from the external surfaces of the probe e Dispensing liquid through the probe into the rinsing station will eliminate internal contamination An alternate internal external cleansing using a non flowing rinse is also possible Internal rinsing may not be required at all if large diluent volumes are dispensed immediately after dispensing of the sample a common practice in dilution procedures Appendix D Minimizing Dilution Effect An undesirable dilution sometimes occurs when an aspirated sample replaces rinse or diluent material Some of the diluent fluid invariably clings to the tubing walls causing the unwanted dilution of the sample The part of the sample that enters the tubing first is affected most because it is the closest to the diluting liquid The corrective action you take depends on the stringency of your requirements for sample purity and precision e You can aspirate an air gap into the
47. probe tip to separate different fluids passing through the tubing to reduce mixing e You can aspirate an amount in excess of the required sample volume dispensing the excess sample which is most subject to being diluted to waste In extreme cases you can combine the methods listed above and perform the following routine to eliminate mixing of different liquids Move probe to sample tube Aspirate air gap Lower probe to sample level Aspirate small amount of sample this is the excess sample volume Lift probe Aspirate air gap Lower probe to sample level Aspirate sample amount AUNE ONOU D 3 sonbiuyse Buijjedid Pipetting Techniques Appendix D 4 D The following example describes an instance when the above 8 amp step procedure is required An operator needs to dispense 50 uL of reagent into each of 20 test tubes The tube to tube consistency of reagent volume is critical However in order to save time the operator wishes to make a single 1 mL aspiration from the reagent reservoir and then make multiple dispenses If the 8 step procedure was not used the last aliquot of the 20 dispenses would be more diluted than the others If the procedure was used the requirements of the routine and the operator s desire to save time could both be met Recommended volumes in this example are 15 uL air gaps and 100 uL excess volume in Step 4
48. r vV Address 0 to 7 of user defined character ss sSs Characters in hexadecimal ASCII code for rows 1 through 7 See example below For example the following command sets the pattern shown below to user defined character address 0 cO 1F 111B151B111 Row Pattern Hex Code 2 x o 11 4 X k 15 6 11 Reads the current error number Returns nnn which identifies the error number see page 5 2 for listing of errors If no error has occurred returns 0 Clears error number Note Whenever possible it is recommended that the immediate command be used to clear errors Appendix Exyz Fn Jcccc p jcttt Sets X Y and Z motor status x Ofor disable X motor y Ofor disable Y motor Z 0 for disable or 1 for enable Z motor For example the following command disables the motors E000 Relaxes the probes where n is the probe selection a b c or d Moves XYZ to home position homes all four Z drives and sets the X pitch to 18 mm Reads status of input contacts and front panel Start and Stop buttons Returns cccckk where cccc Status of input contacts A B C and D 1 if closed shorted 0 if open kk Status of front panel Start and Stop buttons 1 if button has been pressed 0 if it hasn t been pressed Value of kk is cleared after reading Reads status of output contacts Returns ccccp where cccc Status of output contacts 1 2 3 and 4 1 if co
49. r to the liquid handler 2 Locate the appropriate Z haght adjustment tool in the accessory package Two Z height adjustment tools are supplied in the package Part number Description 25051094 125 mm Z height adjustment tool Use this tool to adjust the Z arm on the Quad Z 215 Liquid Handler for liquid handling and injection 25051095 175 mm Z height adjustment tool Use this tool to adjust the Z arm on the Quad Z 215 Liquid Handler for liquid handling 3 Place the Z height adjustment tool on one of its ends near the center of the locator plate if necessary remove any racks or accessories before doing this 2 height adjusiment tool Installation 2 4 Loosen the mounting screw on the Z arm mounting bracket and slightly raise the Z arm mounting screw mounting bracket 5 Manually move the liquid handler s arm so the Z arm is centered over the Z height adjustment tool 6 While holding Z height adjustment tool flat against the locator plate use the other hand to lower the Z arm until it lightly rests on the adjustment tool 7 Tighten the mounting screw on the Z arm mounting bracket so the Z arm is secure 8 While holding the adjustment tool in place slide the Z arm off the tool Ensure that the bottom of the Z arm lightly rubs against the adjustment tool as it moves Repeat steps 4 through 7 until this is true 9 Store the Z height adjustment tool 2 15 juawysnipy wuy Z jeul4 Electrical Connecti
50. range Correct the error in the program controlling the liquid handler Troubleshooting 29 30 31 32 33 34 35 36 39 41 42 43 44 55 56 57 58 59 Z target more than maximum Z X encoder inactive Y encoder inactive Z position sensor inactive Safety contact activated X home phase is invalid Y home phase is invalid X and Y home phases are invalid Stop button has been pressed GSIOC communication error Time out Undefined GSIOC command GSIOC command sequence incorrect Cannot send commands Unit busy Probe A motor position error Probe B motor position error Probe C motor position error Probe D motor position error Pitch motor position error Send the immediate Q command using the GSIOC Utility Program to read the travel range Correct the error in the program controlling the liquid handler Contact the Gilson Customer Service Department Contact the Gilson Customer Service Department Contact the Gilson Customer Service Department Release contact then restart Run 215SETUP EXE or the 215 Setup Utility program to correct the problem Run 215SETUP EXE or the 215 Setup Utility program to correct the problem Run 215SETUP EXE or the 215 Setup Utility program to correct the problem Turn power off then on to the liquid handler Contact the Gilson Customer Service Department Contact the Gilson Customer Service Department Contact the Gilson C
51. reases sensitivity for situations where liquid is harder to detect By lowering the probe so it touches the liquid for one of your samples you can manually test the current sensitivity of the liquid detection In this manner you can observe the amount of change you might expect with each sample The factory default setting is 10 for each probe 3 9 Ja puey pinbiq ay Bunn uos Configuring the Liquid Handler Operation 3 10 Adjust XY THe Home Pe OO a ning Lie bardais Dempey iba Pump Tage Senge Opto Riese Se DdD A Mimik Cites Meas Muciges Kate ie ead TA aia Tahana a os Te a Nem iy p anaa a Esec Cea e The Adjust XY tab allows you to test whether the instrument is properly adjusted and to make minor adjustments to the X and Y axis offsets if needed You may need to use the options under this tab if the probe is not accessing the injection port of the injection module installed or the vessels in the installed racks The X offset and Y offset text boxes display the current offsets stored in the instrument s memory To determine if the probe s need s to be adjusted in the X or Y direction sdect the mode of the injection module that is on your Quad Z 215 or select other for a user defined test point The default XY coordinates are shown next to the model number Select the mode of the injection module that is on your 215 or select other for a user defined test
52. rn OFF power to the Quad Z 215 Dispense a small bead of grease on the applicator Apply the grease to the visible and accessible areas on the vertical rods and horizontal pitch rods on the Z arm refer to the pictures on the next page Repeatedly and slowly move the probe holders up and down to distribute the grease along the vertical rods Using a 2 5 mm hex wrench adjust the pitch to 9 or 18 mm whichever is opposite of the current setting Refer to the diagram at right Insert and turn a 2 5 mm hex wrench to manually adjust the pitch 4 7 Bunesnqn Lubricating Maintenance 4 8 4 6 Dispense another small bead of grease on the applicator 7 Apply the grease to the visible and accessible areas on the vertical rods and horizontal pitch rods on the Z arm see below 8 Repeatedly and slowly move the probe holders up and down to distribute the grease along the vertical rods C horizontal pitch rods vertical rod vertical rod 18 mm spacing 9 mm spacing Maintenance 4 Replacing Parts Replacing Tubing It is important to keep all tubing clean and free of crimps Tubing that has become dirty blocked or crimped can result in poor accuracy and precision or loss of air gap Replace both the transfer tubing and inlet tubing as needed See Appendix A for part numbers for replacement tubing For tubing installation procedures see Section 2 Replacing a Probe Ref
53. sed by improperly packaged instruments Indicate the authorization on the carton and on the packing slip Always insure for the replacement value of the unit Include a description of symptoms your name address phone number and purchase order to cover repair costs return and shipping charges if your institution requires it Ship to Gilson Inc Attention Customer Service indicate the authorization here 3000 W Beltline Highway Middleton WI 53562 Outside the United States contact your Gilson representative for return procedures 59 S 19l Od U1N y4 pue ed y Replacement Parts and Accessories A Probes 2507214 2507215 2507254 25073645 2507253 2507216 Non seotum piercing probe constricted tip capacitive level sensing stainless steel Dimensions 269 x 1 8 x 14mm ID tip dimensions 1 5 x 1 2 x 0 8 mm ID Requires probe guide insert part number 25064473 for precise XY accuracy Non septum piercing probe constricted tip capacitive level sensing stainless steel Dimensions 269 x 1 3 x 0 8 mm ID constricted tip 1 5 x 0 9 x 0 45 mm ID Requires probe guide insert part number 25064471 for precise XY accuracy Non septum piercing probe flat tip capacitive level sensing stainless steel Dimensions 269 x 1 8 x 1 4 mm ID Requires probe guide insert part number 25064473 for precise XY accuracy Non septun piercing probe beveled tip capacitive level sensing stainless steel
54. sert until the tip of the probe is in the probe guide Replace and tighten the 1 4 28 fitting Maintenance _ 4 Replacing a Level Sensing Cable Removing the level sensing cable 1 Detach the transfer tubing from the top of the isolation probe holder by using the supplied headless nut extender part number 49041032 to loosen and remove the 1 4 28 nut and ferrule 2 Unplug the right angle connector for the liquid level aa sensing cable LLD cable from the socket on the back of the Z arm right angle connector 3 Detach the LLD cable from the LLD cable guide wina 4 Loosen the small hexagonal nut on the probe holder for probe one Detach the split tongue connector from the nut 5 Remove the transfer tubing from the LLD cable casing 4 11 sued Buloejdey Replacing Parts Maintenance 4 12 Installing the new level sensing cable 1 Locate the new level sensing cable supplied with the Z arm Pass the transfer tubing through the open end of an LLD cable casing nearest the right angle connector The tubing should exit the back of the LLD cable casing approximately 6 5 cm from the end Connect the transfer tubing to the top of the isolation probe holder using a 1 4 28 nut and ferrule supplied with the tubing Firmly tighten this fitting using the supplied headless nut extender part number 49041032 since it holds the probe in place Plug the right angle connector on one end of the cable into sock
55. sly installed rinse station base located at the rear of the instrument bed To install the rinse station onto the base follow these steps 1 Align the triangle on the bottom of the rinse station with the base 2 With the rinse station s fittings facing you insert the rinse station into the base 3 Press down and turn the rinse station clockwise The rinse station is secure when you feel the rinse station snap into place When installed correctly the fittings point toward the control cabinet 4 Ifyou will be doing shallow or deep pocket rinses connect waste tubing to the barbed fitting installed on the rinse station If you will be doing flow through rinses remove the plug installed on the rinse station and replace it with a barbed fitting Connect tubing between the barbed fitting and the external liquid source Installation 2 Rack Setup The Quad Z 215 Liquid Handler is equipped to locate Code 20 30 200 and 500 series racks See Appendix B for a list of racks available for the liquid handler Depending on the racks you re using refer to the appropriate procedures on the following pages Code 200 Series and Code 500 Series Racks If all your racks are Code 200 series or Code 500 series racks place them directly onto the locator plate 1 Orient the rack so that the code number for example 200 is facing forward 2 Fit the rack on the locator plate so that the slots and holes on the underside
56. te and reproducible results The external 444 QuadDilutor provides for the accurate and precise handling of liquids Introduction 1 Unpacking The Quad Z 215 Liquid Handler is delivered with all major components already assembled except for auxiliary parts such as the Z arm probe racks tubing etc Keep the original container and packing assembly in case the liquid handler must be returned to the factory The Quad Z 215 Liquid Handler and its components are shipped in two containers e One container holds the auxiliary items such as locator plate tubing probes rinse station Z arm and any other accessories you may have ordered with your system e The other container holds the Quad Z 215 Liquid Handler To remove the liquid handler from its container 1 Cut the metal strapping 2 Lift the outer box off and away from the liquid handler 3 Lift the inner box off and away from the liquid handler 4 Lift the unit off its base platform and place it on a lab bench or cart Gilson recommends that two people lift the liquid handler off the base of the packing container To lift the liquid handler a Using the two cutouts for hand holds place a hand at the base of the packing container b Grip the liquid handler under the base plate c Lift the unit up and out of the foam packing material The side containing the electronics cabinet is the heavier side Do not attempt to lift the instrument from the Y arm
57. terial polypropylene Vessels and maximum capacity 17 x 55 mm vials 6 8 mL 17 x 65 mm vials 8 mL Part number 150426 Required accessories adapter plate part number 2504621 Code 24 rack For 14 scintillation vials Material polypropylene Vessels and maximum capacity 28 x 60 mm 20 mL Part number 150427 Required accessories adapter plate part number 2504621 Appendix Code 28 rack For 108 vessels Material polypropylene Vessels and maximum capacity 10 x 65 mm tubes 3 mL 10 x 75 mm tubes 3 5 mL Part number 150420 Required accessories adapter plate part number 2504621 Code 29 rack For 60 vessels Material polypropylene Vessels and maximum capacity 12 x 75 mm tubes 5 mL 13 x 75 mm tubes 6 mL Part number 150429 Required accessories adapter plate part number 2504621 Code 30 rack For 60 vessels Material aluminum Vessels and maximum capacity 12 x 32 mm vials 2 mL Part number 2704430 Required accessories adapter plate part number 2504621 and thermostating cuvette part number 2704429 or thermostating cuvette part number 2759550 and 832 Temperature Regulator part number 2759502 Code 31 rack Thermostated rack for 108 vessels Material aluminum Vessels and maximum capacity 7 x 40 mm vials 0 7 mL Part number 2704431 Required accessories adapter plate part number 2504621 and thermostating cuvette part number 2704429 or thermostating cuvette part number 275
58. tion stripper e small blade screwdriver You can purchase a 6 foot piece of suitable cable part number 709910206 or a package of five cables with identification markers part number 36078155 from Gilson 2 17 SUOI OBUUOD e91 99 9 Electrical Connections Installation 2 18 Dormin bik COTE Paa ja e aj cable io peripheral Speen Making connections To prepare and make connections with the 2 conductor cable 1 Cut the cable into pieces of appropriate length 2 Strip about 0 25 cm of insulation from each end of the cable 3 Remove the terminal block connector from the liquid handler Insert each wire into the appropriate slot on the terminal block connector Note When making connections be sure to maintain the correct orientation of the connector relative to the port Push the wire all the way in then tighten its corresponding pin screw 4 Reconnect the terminal block connector to the liquid handler The wires will be facing left and the pin screws will be facing you as you look at the rear of the instrument Push the connector in as far as it will go It is designed to fit snugly into its receptacle 5 Connect the opposite ends of the wires to the other device s Be sure to match ground connections 6 Label each cable to identify the purpose of the connection Installation 2 RS 232 Port The RS 232 port is used to transfer information between the liquid handler and a computer For the l
59. tions This type of insert allows for a deeper insertion of the probe into for tegoa bain the rinse well resulting in a to waste bottle greater area of the outside plug for flow through of the probe to be rinsed rinse station replace plug with a barbed fitting y nd nect tubi e Flow through rinse Used between fie Ming and in applications where a the extemal liquid source rigorous wash of the probe s exterior is required ports for A second source of liquid is pumped to the rinse station to perform this type of rinse Mi orate It may be necessary to vary the types and P ooVeses ports for volumes of probe wash solutions to most 4 EIK J ee efficiently eliminate carryover of particular compounds Generally the smaller the volume of probe wash solution used the faster your automated liquid handling protocol 2 9 SUOI D9UUOZD Huiquinid Plumbing Connections Installation J gt a a 2 10 pins to secure ninse staton bases 9 Af Jf XA ff kd kd locator plate sl holes to secure Installing the rinse station The base of one rinse station is shipped already secured to the locator plate and is located at the rear of the locator plate The locator plate can hold an optional second rinse station or you can move the rinse station base to the alternate location Before installing the rinse station make sure the locator plate has been properly installed with the previou
60. tire fluid path has been flushed with water end the priming sequence 45 BHuiues D Cleaning Maintenance 46 Biological fluids Follow this procedure if the fluid path is in contact with biological fluids such as blood products 1 Make a solution of 10 bleach by adding one part of commercial bleach to nine parts of water Prime the fluid path using the bleach solution until the entire fluid path has come into contact with bleach Pause the priming sequence After 30 minutes resume priming the fluid path using distilled or deionized water to pump the remaining bleach solution from the tubing into a waste container Prime the fluid path a minimum of 10 cydes with distilled or deionized water When you re satisfied that the entire fluid path has been flushed with water end the priming sequence Maintenance Lubricating Always switch the power to off when making adjustments to such as lubricating the liquid handler The potential exists for bodily harm if you interfere with the work area of the instrument while it is running Horizontal Pitch and Vertical Rods Refer to the instructions and diagrams on the next few pages for information on lubricating the horizontal pitch and vertical rods on the Quad Z 215 What you need Mobile 007 grease part number 25494101 included in the 215 Alignment Kit part number 254941 Applicator a cotton swab for example Procedures 1 2 Tu
61. tor on the rear pane and turn it 2 Align the white dot with one of the indicated numbers The unit ID is 20 plus the selected number 2 21 SUOI OBUUOD e Electrical Connections Installation 2 22 Baud rate mode As a default the baud rate mode is set to 6 indicating that the liquid handler is set for a baud rate of 19200 and is a master device You ll need to change the selection to O zero if the liquid handler is connected via the GSIOC toa Gilson system and is being controlled by the 506C System Interface from Gilson control software A setting of 0 indicates the liquid handler is a slave device and the baud rate is being clocked externally Note that other selections are available if the liquid handler is being controlled by non Gilson applications Refer to the table shown on the liquid handler s rear panel see page 2 16 To change the baud rate mode 1 Gently insert a small flat blade screwdriver into the SW2 selector on the rear panel and turn it 2 Align the white dot with one of the indicated numbers Installation Fuses You received the liquid handler without any fuses installed To install the fuses 1 Locate the accessory package containing the fuse drawer appropriate for your line voltage Discard the other fuse drawer 2 Locate the accessory package containing the 5 0A T Slo Blo fuse 5 x 20 mm size fuses 3 Install the fuse s into the fuse drawer The fuse drawer for 1
62. ubes Material aluminum Vessels and maximum capacity 96 10 x 75 mm culture tubes 3 5 mL Part number 2504617 Code 218 rack For two microplates and eight 10 x 75 culture tubes Material aluminum Vessels and maximum capacity two 96 well microplates and eight 10 x 75 mm culture tubes 3 5 mL Part number 2504618 Note Instead of microplate s you can install Beckman modular reservoirs B 9 syory Racks B 10 Code 222 rack For 27 conical bottom tubes Material aluminum Vessels and maximum capacity 27 50 mL Part number 2504622 Code 228 rack For four reagent bottles Material aluminum Vessels and maximum capacity 4 500 or 700 mL Part number 2504628 Code 505 rack For 10 standard or deep well microplates Material aluminum Part number 2504651 Code 505H rack For 10 standard or deep well microplates with hold down covers Material aluminum Part number 2504651H Appendix Peltier Racks Code 242 Peltier rack For 2 shallow flat bottom 96 well microplates Material aluminum Vessels and maximum capacity 2 shallow flat bottom 96 well microplates Part number 25146331 Note Order Peltier Controller part number 2515331 and junction box part number 2505332 separately Code 542 Peltier rack For 10 shallow flat bottom 96 well microplates Material aluminum Vessels and maximum capacity 10 shallow flat bottom 96 well microplates Part number 2514542 Note
63. ustomer Service Department Contact the Gilson Customer Service Department Turn power off then on to the liquid handler Turn power off then on to the liquid handler Turn power off then on to the liquid handler Turn power off then on to the liquid handler Turn power off then on to the liquid handler 5 3 sobessay 10119 Mechanical Troubleshooting 5 4 Mechanical Probe s no longer finding tube center Probe s may be bent Straighten or replace the probe Incorrect tray file defined Review and if necessary change the tray file Liquid handler may be misaligned Perform the position alignment procedures described on page 414 Troubleshooting 5 Electrical Input functions not operating jeouj09 3 Make sure connections into terminal block connector are secure Make sure terminal block connector is secure in input output port Check connections for proper pin assignments Be sure pins from external devices are assigned correctly Check polarity of input Inputs should bea contact closure If not it must be TTL level logic O activates Confirm that source supplying input to liquid handler is working Output functions not operating Make sure connections into terminal block connector are secure Make sure terminal block connector is secure in the input output port Check connections for proper pin assignments Output from liquid handler should be compatible with device to which

Gilson 215 User's Manual

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