VistaVision Inverted Microscope Manual
Contents
1. Turn on the toggle switch Q on the right of the body frame so that the transmitted halogen bulb is illuminated Turn the brightness control knob to adjust the brightness of the bulb and make the brightness of the field of view suitable for visual inspection As shown in Fig 5 Do not keep the brightness control knob at the brightest position for a prolonged period of time otherwise the life of the bulb may be shortened When this unit is not in use turn the brightness adjusting knob to the low position to maintain the electric functions of this unit 2 Adjustment of the condenser height Before this unit is released from the factory the condenser already bears a calibrated height mark 1 Turn the condenser height control knob to move the condenser into working position until the two lines on the condenser coincide which is optimum position for most applications as shown in Fig 6 Operating Manual of Inverted Microscope 3 Adjustment of phase contrast device Set the phase contrast puller to the central light hole as shown in Fig 7 Fig 7 4 Reset of diopter adjustment ring Turn the diopter adjustment ring on the left eyepiece tube so that the 0 diopter position is aligned with the side scale remark white line as shown in Fig 8 5 Adjustment of interpupillary distance Parallax can be eliminated by adjusting the interpupillary distance so that the distance of the eyepiece tube is ide2. Inverted Microscope Model 2026 630 Operating Manual istaVision Operating Manual of Inverted Microscope Congratulations Thank You for your purchase of a VWR VistaVision Microscope This unit is a precision optical instrument Our product has been design to provide the highest level of safety However improper operation or negligence in following the instructions in this manual may cause personal injuries and property losses In order to ensure your safety to maximize the life of this unit and to maintain it properly please read through this manual carefully before operating this unit Operating Manual of Inverted Microscope Safety Precautions amp Reminder 1 Be sure to turn off the power switch and remove the power cord plug before installing this unit replacing the bulb fuse or plugging and unplugging the power supply unit This is to prevent electric shock or getting fingers burnt 2 Check unit voltage is in compliance with your local power supply voltage otherwise contact your supplier Improper input voltage may render unit not workable or a short circuit and damage the unit 3 Use specified bulb fuse and power cord which otherwise may damage the unit Any extended power cord if used must be grounded PE 4 Do not expose unit to high temperatures and dampness Suitable operating environment is between 5 C to 35 C and relative humidity of 20 80 at 25 C Do not subject unit to direct sunlight
3. turn off the power supply of this unit allow the bulb to cool down sufficiently put on the dust shield store this unit at a dry ventilated and clean place free from any acid alkali or steam otherwise mold may develop on the lens 6 Periodic inspection This unit should be inspected and maintained periodically to maintain its performance Do not wipe this unit with any organic solvent e g alcohol ethyl ether or its dilute solution otherwise the surface paint of this unit may come off It is suggested that a layer of non corrosive lubricant is applied on the moving parts of this unit before the dust shield is put on and place the eyepiece and the objectives in a container with desiccant VIII Troubleshooting The power switch is not turned on Turn on the power switch The halogen lamp is damaged Replace the halogen lamp ne vem a en The fuse is damaged Replace the fuse view using halogen lamp ve C OME LOT Di TAE ea michal Check and have a professional repair it in bad contact oe N ApmOnRIEdiE Use a conforming halogen lamp nonconforming Optical system and imaging Te E oe nn 0 Turn the nosepiece to the fixed position the fixed position There is a black shadow on the The filament image deviates from the Ba al ohene bull edge of the field of view or center of the collector aaa a Zn unevenly illuminated making it impossible to observe the whole There is dirt or oil on the surface of the etna ones EU lene fi
4. and hold it stably The phase contrast objective does not match the ring plate of the phase contrast condenser lens The center of the ring plate of the Align the phase contrast objective with the mark on the turntable of the phase contrast condenser lens Center the phase contrast condenser lens so that the ring plate coincides with the phase plate The effect of phase contrast observation is bad phase contrast condenser lens deviates from that of the phase plate of the phase contrast objective The specimen used is not suitable for Replace it with one suitable for phase contrast observation phase contrast observation Mechanical system Tne tocunng imee anem u Adjust the coarse adjusting hand wheel down automatically a en Lena one The fine focusing mechanism fails Check and have a professional repair it uring observation The stage loosens or is inclined Check and have a professional repair it BR The lock screws loosen making When the condenser lifting positioning difticule Recalibrate the lock screws device swings in or out the fixed position cannot be located The locking mechanism is seriously precisely or is insecure worn and has insufficient clamping Check and have a professional repair it force 16
5. 5 Do not touch the halogen lamp box when using the unit as bulbs inside generate excess heat which can burn your hand Do not handle bulb with bare hand within 10 minutes after switching off power supply as bulb is still hot especially when changing a defective bulb 6 To prevent fire do not place any fibrous product paper flammable or explosive material e g gasoline petroleum ether alcohol near the halogen lamp box 7 When using coarse fine focus knobs or height adjustment knob do not continue to turn them beyond the limits or the internal mechanism can be damaged Operating Manual of Inverted Microscope Table of Contents L Characteristies and applications asus 1 LD Struet ralteatures uses 1 Hi Installation of this Unit nee Be arstosieealemannnere teenie eres 2 IN LECHMICALSPECINICALIONS araisa einai nun auumn oem unnmmmuanmmatenaemets ee 4 Vo OPTa ons Neuen 5 VI Replacement of bulb and Tuse u an 13 NAV IVE AICO ANCE as ur 14 VUES TrouDleshoolnp 2 0 00 Reese insel 15 Operating Manual of Inverted Microscope I Characteristics and applications The inverted microscope adopts an inverted structure that focuses by lifting or lowering the objectives and is provided with long working distance plan objectives and Wide field plan eyepieces The long working distance condenser system can be placed into or out of the light path and is suitable for tissue observation in a high culture dish The pha
6. Fig 17 2 If observing tissues in a big culture dish the stage moving scale D on the fixed stage must be removed During removal keep the condenser system out of the light path remove the stage bracket and lower the objective turn the coarse focusing control knob so that the objective lifting mechanism is lowered Use a screwdriver to loosen the 3 mounting screws at the bottom of the stage moving device remove the stage moving device and lay it flatly on the worktable upside down Do not lay it laterally or overhead otherwise it may drop or be deformed affecting the precision of this device See Fig 18 for the operating process Operating Manual of Inverted Microscope The stage moving device is the key part for carrying the specimen and the culture dish Do not lay it laterally or overhead after removal otherwise it is likely to drop affecting its precision It should be laid flatly on the worktable upside down 3 Diopter adjustment Diopter can be adjusted by the diopter adjusting ring on the left eyepiece tube which can correct any diopter difference between both eyes of different users A Turn the 40X objective into the light path and observe the specimen image in the right eyepiece the eyepiece in the eyepiece tube without the diopter adjusting ring with the right eye alone and focus until a clear image is seen B Observe the specimen image in the left eyepiece with the left eye If the image is
7. IV Technical specifications Total magnification 100X 400X standard Mechanical tube Main parameters 160mm ength Conjugate distance ea 195mm of objective field of view en WE 10X Eyepiece Wide field plan number 20mm Parfocal distance Eyepiece l l interface eyepiece field of view 10mm WF 16X optional 23 2mm number 11mm Hinged binocular tube with an observation angle of 30 and a pupil distance of 53 75mm f Thickness of Numerical Magnification cover glass Remarks aperture gt O Phase contrast a 25X 0 40 4 78 1 2 Remark PHP2 objective Ultra long working distance condenser Working distance 55mm with phase contrast device long working distance condenser l l Condenser l Working distance 35mm with phase contrast device optional Long working distance condenser Working distance 70mm optional Mechanical Stage moving range 79mm longitudinal X 130mm transverse detachable stage Working distance Stage moving device Holder 1 86mm W X129 5mm L optional with a circular culture dish 87 5mm Stage bracket Holder 2 34mm W X77 5mm L optional with a circular culture dish 068 5mm Holder 3 57mm W X82mm L Transmitted Shc Light source licht 6V 30W halogen lamp adjustable in brightness ighting Operating Manual of Inverted Microscope V Operation Inverted Microscope Operation 1 Turning on the power switch and brightness adjustment 5 Fig 5
8. aged fuse and replace a new one Replace the fuse holder into the power input socket of the main unit as shown in Fig 24 3 Reconnect the power cord and check if the fuse tube is in good contact Fig 24 VII Maintenance 1 The power switch of the main unit is the power control When finish using this unit press the switch to OQ to cut off the power supply unless the electric components in this unit is still operating When this unit is not to be used for a long time remove the power plug from the supply socket and keep all cables properly 2 This unit should be kept clean Remove any oil on the lens and clean the body with clean gauze or silk fabric or absorbent cotton dipped with a little alcohol Put on the dust shield until this unit 1s completely cool and dry 3 Cleaning the lens Blow off or wipe off any dust on the lens with a blower ball or a soft brush heavy dirt and fingerprints can be removed with lens tissue or soft cloth dipped with a little mixture of alcohol and ethyl ether gently the mix ratio is alcohol 20 30 and ethyl ether 70 80 Reminder It is easier to clean the lens by wiping minder re them from inside out as shown in the figure Wrong Right 4 Cleaning the surface of this unit Wipe it with clean soft cloth heavy dirt may be wiped off with a neutral detergent 14 Operating Manual of Inverted Microscope 5 Keeping When this unit is not to be used for a long time
9. b and the fuse turn off the power switch and unplug the power cord otherwise fire personal injury or damage to this unit may occur due to electric short circuit Halogen Lamp Replacement 1 Turn off the power switch and unplug the power cord as shown in Fig 23 a 23 b 2 Wait at least 10 minutes until the bulb and its surroundings have cool down This is to prevent hand getting burnt by heat 3 Unscrew the screw loosen the screw and pull out the lamp holder from the halogen lamp housing Use a cloth to remove the defective bulb as it may still be very hot after use Replace the halogen bulb and place back the lamp holder into position The bulb feet should be inserted as deeply as possible otherwise the life of the bulb may be shortened due to bad contact or the bulb may not be illuminated As shown in Fig 23 d 4 Check and adjust the center of the bulb according to the above mentioned centering method for the alignment of illuminator in the inverted microscope operation Ore Fig 23 13 Operating Manual of Inverted Microscope Replacement Of The Main Unit Fuse The fuse of the main unit is a circuit system used for halogen lamp and is integrated into the power input socket 8 of the main unit 1 Turn off the power switch Q and unplug the power cord as shown in Fig 24 2 Remove the fuse holder with a flat screwdriver D or any other tool as shown in the figure remove the dam
10. bulb must be removed as shown in Fig 13 This cannot be done until the bulb is cool Wrap the bulb with a cotton fabric 8 and adjust the bulb vertically gently in the direction indicated by the arrow in Fig 13 This can center the bulb front and rear Do not adjust the bulb too hard as this will add to the difficulty of precise adjustment The bulb should not be removed for adjustment until it is cool Do not pull the bulb too hard otherwise the feet may burst 7 Placement of specimen slice or culture dish 1 To observe a slide specimen Q the slide can be placed directly on the stage bracket To observe tissues in a culture dish determine the stage bracket to be used based on the external dimensions of the culture dish refer to the technical specification table for the allowable culture dish dimensions as shown in Fig 14 Operating Manual of Inverted Microscope 2 Adjust the stage moving device control knob and so that the observed area is right above the objective for easy observation and adjustment as shown in Fig 15 Fig 15 8 Coarse and fine focusing control knob operation 1 Focus with the 10X objective Turn the nosepiece to move the 10X objective into the light path when it turns in place the objective will snap automatically as shown in Fig 16 2 Turn the coarse focusing control knob to lift the objective to the highest point Then observe through the eyepiece Tu
11. dge of the dark rings and bright rings is unclear in the field of view the adjusting ring of the centering eyepiece can be adjusted to make the edge clear 4 In the phase contrast observation mode the center of the dark ring should coincide with that of the bright ring If their centers do not coincide as shown in Fig 20 c it is necessary to center the phase contrast This can be done by adjusting the centering screws 8 of the puller phase contrast The adjusted the field of view is as shown in Fig 20 d 5 After the phase contrast device is centered the centering eyepiece can be removed and the working eyepiece inserted again In case of changeover to a phase contrast objective of a different magnification the phase contrast device must be centered again otherwise the effect of observation phase contrast would be affected Fig 20 _ 2 Operating Manual of Inverted Microscope Operation of Trinocular Device This unit performs visual inspection and photographic observation through push pull changeover 1 Loosen the fastening screws 2 of the photographic output tube and remove the dust cap of the photographic output tube as shown on Fig 21 2 Mount the photographic device on the output tube and then tighten the fastening screws 3 Pull out the photographic visual changeover pusher 8 the image will show in the monitor or display screen VI Replacement of bulb and fuse Warning To replace the bul
12. eld of view objective eyepiece or condenser P P The field of view diaphragm is not Open thadieid Se view hm open completely Oil or dust is found in the field of There is oil or dust on the eyepiece Wine th view lens per een 15 Operating Manual of Inverted Microscope There s oil or any foreign object on the edge of the field of view Wipe the diaphragm edge diaphragm The objective is damaged Repair the objective by a professional There is oil or dust on the surface of the lens of the objective or eyepiece Wipe the objective or the eyepiece Adjust the aperture of the aperture The aperture of the aperture diaphragm diaphragm based on the objective is too small magnification or numerical aperture used ODIEN Covidtes momma NEN Turn the nosepiece to the fixed position Offset focal plan or low resolution The specimen cover glass is too thick Load a cover glass as necessary for the or thin objective The upper and lower sides of the Place the upper and lower sides of the specimen are inverted specimen positively The oil has not bee wiped off timely and remains for a prolonged period of time Wipe the front lens of the objective The focal plane of the image is The lighting bulb is seriously inclined Reposition the lighting bulb inclined brighter on one side and darker on the other Lay the specimen flatly on the object stage The specimen is not laid flatly
13. nclear it can be made clear by adjusting the condenser height control knob C If the light spot deviates from the center of the field of view as shown in Fig 10 a the condenser center center of aperture diaphragm must be adjusted The center of the light spot can coincide with that of the field of view by adjusting two the centering screws 8 of the condenser holder as shown in Fig 10 b D Open the field diaphragm so that the observed specimen image fills the visual field as shown in Fig 10 c Fig 10 2 Inspection of alignment of illuminator A Prepare a piece of white paper about 40mmX50mm 8 unscrew the filter holder on the phase contrast condenser and place the white paper on the top of the phase contrast condenser as shown in Operating Manual of Inverted Microscope B At this point a bright light spot will be shown on the white paper with a filament image inside If the filament image is unclear it can be made clear by adjusting the collector lens adjusting handle C If the filament image deviates from the center of the bright light spot as shown in Fig 12 a the bulb must be centered To do this loosen the screws 7 and move the lamp holder 6 gently adjust the left right centering of the bulb as indicated by the arrow in the figure Fig 12 D If it is necessary to center the bulb front and rear as indicated by the arrow in the Fig 12 the whole lamp holder 40 and the
14. ntical with your interpupillary distance and enable you to observe more comfortably and clearly When you observe through two eyepieces if the field of view consists of two overlapping circles as shown Fig 8 a you can alter the exit pupil center distance of the eyepiece tubes by turning the left or right frame body until the field of view becomes a fully overlapped circle as shown in Fig 8 b em i i Fig 8 6 Inspection of transmitted lighting path The optical system of this unit has been calibrated before factory release But the center of the lighting bulb or condenser system may deviate from the center of the optical system due to any possible violent vibration or inclination during transport After the above installation and adjustment the light path of the optical system should be checked 1 Inspection of center of field diaphragm A Put the test slide as an example in the selected Stage bracket Turn the 10X plan objective 8 into the light path Turn the stage moving device control knob and and to move the slide right above the 10X objective Adjust the focus by the coarse and fine focus knobs and D to make the image of slide in the field of view clear As shown Fig 9 Operating Manual of Inverted Microscope Fig 9 B open the aperture diaphragm and close the small field diaphragm Q when a light spot as shown in Fig 11 a can be seen in the field of view If the edge of the light spot is u
15. rn the coarse focusing control knob slowly to lower the objective When a specimen image appears in the field of view stop turning the coarse focusing control knob as shown in Fig 16 3 Turn the fine focusing control knob for fine focusing to make the specimen image clear as shown in Fig 16 4 Lock the objective lifting limit hand wheel as indicated by the arrow in the figure as shown in Fig 16 When you re going to use a high power objective first use the 10X objective Reminder to focus and set the limit hand wheel When mounting a high power l objective raise the objective to the limit height with a coarse focusing control knob directly and then focus finely with the fine focusing control knob Operating Manual of Inverted Microscope 9 Adjustment assembly and removal of stage moving device 1 The longitudinal Y and transverse X movement of the stage moving device is realized by the longitudinal adjusting knob and the transverse adjusting knob 8 that are coaxial as shown in Fig 17 The stage moving device is driven by gear and rack In case of longitudinal or transverse movement to the fixed position maximum do not continue to turn the adjusting knob as this is likely to damage the movement adjusting mechanism When the stage moving device protrudes from the fixed stage the rack will extend outward Be careful not to let the rack hit your hand or any other part of your body
16. se contrast device can be attached to the light path to perform transmitted phase contrast microscopic observation This unit is suitable for the microscopic observation of cells tissues and clear liquid tissues and the dynamic microscopic observation of tissues cultured in a culture dish and is applicable to scientific research institutions colleges and universities medical and health inspection and quarantine agencies agricultural animal husbandry and dairy industries II Structural features 9 K q Er Fig 1 1 Eyepiece 2 Trinocular head 3 Phase contrast condenser 4 Halogen lamp box 5 Body frame 6 Mechanical stage 7 Objective 8 Nosepiece 9 Coarse fine focusing knob 10 Eyepiece tube Operating Manual of Inverted Microscope III Installation Canton Before installing be sure every components is clean no score any parts or glass surface Operating Manual of Inverted Microscope There are 2 styrofoam boxes within the carton box a The first box contains 6 objectives 3 pcs plan objectives 10X 25X amp 40X with single colored ring and 3 pcs plan phase contrast 10X 25X amp 40X with double colored rings and marked PHP2 1 trinocular 2 eyepieces 1 centering telescope 3 filters 1 spare fuse andl spare lamp b The Second box contains 1 body frame 1 phase contrast condenser 3 different size stage brackets holders Installation steps The number in the following installation s
17. teps is shown in the installing diagram such as 1 is Body in Fig 2 1 Unpack this unit take out the body frame 1 and place it on a stable work bench and remove the supporting package and the dust cap bag 2 Take out the phase contrast condenser 4 Loosen the screw on the condenser holder 3 and then install the condenser into the holder tighten the screw again As shown in Fig 3 3 Take out the stage bracket 2 when the stage bracket is used to carry a specimen or observe tissue in the culture dish put the desired specification stage bracket flatly in the stage moving device rack As shown in Fig 3 Q Fig 3 4 Remove the dust cap of eyepiece tube insert the eyepiece 5 into the eyepiece tube and turn the eyepieces so that it fits the eyepiece tube well 5 There are 6 objectives Those with single colored ring are plan objectives and with double colored rings are phase contrast objectives which are also marked PHP2 Install them onto the nose piece User can only mount 5 objectives onto the nose piece at any one time Install them clockwise in the ascending order of magnification As shown in Fig 4 Fig 4 6 Connect the power cord to the power supply socket of the body 7 Check if the above installation is secure and safe 8 Inspect and gather the accessories and tools enclosed in the package and keep them in a safe place to avoid misplacement Operating Manual of Inverted Microscope
18. unclear it is necessary to adjust the diopter adjusting ring so that the left eye can also see a clear image The diopter adjusting range of this unit is N 5 diopters as shown in Fig 19 Fig 19 Phase Contrast Microscopic Observation 1 Adjustment of phase contrast device Turn the 10X phase contrast objective into the light path and turn the phase contrast turntable or phase contrast puller to the 10 scale Turn condenser height control knob so that the phase contrast condenser rises to the position of scale In the phase contrast observation mode the phase contrast objective must Reminder correspond to the phase contrast ring plate in the phase contrast condenser eminder j RR u 1 e the magnification of the objective corresponds to the scale of the puller or insert plate otherwise the imaging effect of phase contrast observation would be affected 2 Positioning of specimen Lay the phase contrast specimen flatly on the stage bracket see Fig 14 or directly on the fixed stage remove stage moving device Focus to make the specimen image clear 41 Operating Manual of Inverted Microscope 3 Centering of phase contrast device 1 Remove one eyepiece from the eyepiece tube and insert the centering eyepiece into the eyepiece tube as shown in Fig 20 a 2 A dark ring and a bright ring can be observed in the field of view of the centering eyepiece as shown in Fig 20 c 3 If the e
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