operation (continued)
Contents
1. Before using the incubator read the entire manual to understand how to install operate and maintain the incubator in a safe manner Keep this manual available for use by all incubator operators Ensure that all operators are given appropriate training before the incubator begins service GENERAL SAFETY CONSIDERATIONS Note Failure to follow the guidelines and instructions in this manual may create a protection impairment by disabling or interfering with the unit safety features This can result in injury or death Your unit and its recommended accessories are designed and tested to meet strict safety requirements It is designed to connect to a power source using the specific power cord type shipped with the unit For continued safe operation of your incubator always follow basic safety precautions including e Always plug the incubator power cord into a protective earth grounded electrical receptacle outlet that conforms to national and local electrical codes If the incubator is not grounded properly parts such as knobs and controls can conduct electricity and cause serious injury e Avoid damaging the power cord Do not bend it excessively step on it or place heavy objects on it A damaged cord can be a shock or fire hazard Never use a power cord if it is damaged e Always position the unit so that the user can quickly unplug it in the event of an emergency e Do not attempt to move the unit while in operation or before the unit2. 0 1 C and 0 1 CO The devices should be regularly calibrated preferably by a third party For best temperature results use a digital device with a wired connected temperature sensing probe that can be placed in the incubation chamber through the unit access port For example a wire thermocouple probe For best CO accuracy use a Calibrated digital gas analyzer with sample tubing that can be connected to the incubator external CO sample port Reference readings that avoid chamber door openings during verification and calibration eliminate subsequent waits for the chamber temperature and gas levels to re stabilize before proceeding This also allows temperature and gas verifications or calibrations to be performed simultaneously oelect probes suitable for the application temperature you will be calibrating or verifying the incubator displays at Alcohol thermometers do not have sufficient accuracy for conducting accurate temperature verifications and calibrations Do not use a mercury thermometer Never place a mercury thermometer in the incubation chamber 10 Page LAB RECEIVING CONTINUED Page left blank Da IA LAB INSTALLATION AMBIENT CONDITIONS The SCO6AD is intended for use indoors at room temperatures between 15 C and 30 C 59 F and 86 F at no greater than an ambient 80 Relative Humidity at 25 C 77 F Allow a minimum of 4 inches 10cm between the incubator and walls or partitions
3. DISPLAY Note Performing a temperature display calibration requires a temperature reference device Please see the Reference Sensor Devices entry on page 10 for device requirements Temperature calibrations are performed to match the incubator temperature display to the actual air temperature inside the incubation chamber The actual air temperature is supplied by a calibrated reference sensor device Calibrations compensate for drifts in the unit microprocessor controller as well as those caused by the natural material evolution of the sensor probe in the humidified and heated chamber space Calibrate as often as required by your laboratory or production protocol or regulatory compliance schedule Humidity Humidity affects the incubation chamber temperature uniformity Calibrate with the chamber humidified CO2 A CO calibration may be conducted simultaneously with a temperature calibration as long as the chamber door is not opened during either procedure Probe Reference device sensing probes may be introduced through the access port Carefully seat the port stopper over any probe wires or use non stick non marking tape to seal any gaps if there is no room for the stopper Use non stick non marking tape to secure the wires and probe head Place the sensor probe of the temperature reference device inside as close as possible to the geometric center of the chamber A thermocouple sensor probe sleeve may be taped to the shelv
4. If the relative humidity in the incubation chamber is already near the dew point barometric fluctuations may push it across the dew point threshold Note Thin air at higher altitudes holds less humidity than the denser air found at or near sea level If condensation is forming in the incubation chamber wipe down and dry the chamber surfaces then check the following e ls the access port stopper properly seated in the port on the outside of the unit The SCO6AD depends on a controlled atmospheric leak rate through its copper filtered access port to help prevent condensation Stopping up the port by placing the stopper in the port inside the chamber interferes with this leak rate e Make sure samples and items are as evenly spaced as possible for good air circulation There should be at least 1 inch 2 5cm between samples and the chamber walls and door e Are frequent or lengthy chamber door openings causing significant temperature disruptions If so reduce the number of openings Utilize the glass viewing door when conducting visual inspections of samples e Are the viewing and chamber doors closing and latching properly Are the door gaskets leaking Check each gasket for damage wear or signs of brittleness or dryness Replace gaskets if needed e Are there are too many open or breathable containers of evaporating sample media in the chamber If so reduce the number of sample containers e Is the incubator exposed to an exter
5. Steel 1 Tubing Ki Shelf Slides Shelf Standards E 5121028 5170646 9710500 A silicon rubber stopper should come installed in the 1 5 inch 3 8 cm copper filtered access port on the right side of the unit Verify the presence of the stopper 7750565 a Page LAB RECEIVING CONTINUED ORIENTATION PHOTO Side Access Port Back of Unit ei ets Fuse in Power Inlet CO In Port Fuse Control Panel Incubation Chamber Holder Chamber Door Gasket Side of Unit Alarm Dry Contact CO Sample Port USB Style Serial Port Lo Exterior Door Shelf Standard Mounting Rail Viewing Door Latch Viewing Door Gasket Figure 1 SCO6AD Humidification Pan with Copper Token LAB 9 Page RECEIVING CONTINUED RECORDING DATA PLATE INFORMATION MEE Cad i AB com Locate the data plate on the right side of the unit above the copper filtered access port The data plate contains the incubator model number and serial number Enter this information below for future reference Se 1 www SHELL 1 800 322 4898 Date Plate Information Figure 2 Data Plate Model Number Serial Number REFERENCE SENSOR DEVICES Reference sensor devices or a combined device must be purchased separately for performing display accuracy verifications or calibrations for temperature and CO concentration Reference devices must be accurate to at least
6. and 2 inches 5cm of clearance above the top of the incubator for unobstructed airflow Operating the unit outside these conditions may adversely affect the incubator temperature performance including its effective operating range stability and uniformity LOCATION When selecting a location to install the incubator consider all environmental conditions that can affect the effective temperature range uniformity and stability of the unit For example e Ovens autoclaves and any device that produces significant radiant heat e Heating and cooling ducts or other sources of fast moving air currents e High traffic areas e Direct sunlight POWER SOURCE REQUIREMENTS When selecting a location for the unit verify that each of the following requirements are satisfied Wall power sources must match the voltage and ampere requirements listed on the unit data plate This unit is intended for 110 120 VAC 50 60 Hz applications at the following amperage 12 Amps e The wall power source must conform to all national and local electrical codes e Wall power sources must be protective earth grounded Use a separate circuit to prevent loss of product due to overloading or circuit failure e Supplied voltage must not vary more than 10 from the data plate rating Damage to the unit may result if supplied voltage varies more than 10 W The unit comes provided with a 125 volt 15Amp 9ft 5 in 2 86m NEMA 5 15P power cord e The unit must
7. be positioned so that all end users can quickly unplug the power cord in the event of an emergency e Each SCOGAD is provided with a 12 5 Amp 250V Type H 5x20mm fuse located in the power cord inlet on the back of the incubator 12 Page LAB INSTALLATION CONTINUED LIFTING AND HANDLING The unit should only be lifted by its bottom surfaces using proper heavy lifting machinery such as a forklift or pallet jack Handles and knobs are inadequate for lifting or stabilization The unit should be completely restrained from tipping during lifting Transporting the unit while lifted is not recommended and may be hazardous Remove all moving parts such as shelves and trays and secure the door in the closed position prior to lifting the unit Do not attempt to move the unit while in operation or before the unit has cooled LEVELING The unit must be level and stable for safe operation Each incubator ships with four leveling feet Insert one leveling foot into each of the four holes in the bottom corners of the unit Adjust the foot at each corner until the unit stands level and solid without rocking To raise a foot turn it in a counterclockwise direction to lower a foot turn it in a clockwise direction ACCESS PORT STOPPER Each SCO6AD is provided with an access port located on the right side of the unit The incubator is shipped with 1 silicon access port stopper installed in the o copper filtered port on the
8. failed controller board can be repaired or replaced LAB 21 Page OPERATION CONTINUED PREPARING THE INCUBATOR FOR USE Note The preparation procedure requires approximately 20 hours to complete This includes at least 8 hours for the incubator to achieve and stabilize at its temperature humidity and CO2 levels as well as 8 hours to run an auto decontamination cycle setting up the incubator for use in a new workspace environment requires an 8 hour period for the unit to come up to and stabilize at temperature and humidity levels prior to loading the incubation chamber with samples During this period the incubator must be powered continually the humidification pan filled and both the chamber and viewing doors closed COz may be supplied to the unit 2 hours prior to the completion of this stabilization period Allowing time for stabilization helps protect samples It is also necessary for the optional temperature and CO display accuracy verification procedures as well as any calibrations performed Perform the following steps and procedures to prepare the SCO6AD for use each time it is installed in a new workspace environment 1 Optional A clean and disinfected thermocouple probe for performing the optional temperature display accuracy verification may be inserted through the access port now This saves time by allowing the unit chamber temperature to stabilize undisturbed prior to the verification procedure a Seethe T
9. lzgg EE wie 12 AMDI COV OVS A M 12 Koc o 12 Power SOUICO FCO UICTICING NR eevee T E oise that erie a 12 LUPARI o M E 13 OV CTO Y 13 ACCESS FO iin 6 6 0 E ieri 13 WiStall INGUDAION IN LOCANON iii piaci 13 Deionized and Distilled Water iii 13 Installation Cleaning and Disinfection ss 14 Shelving IMStala m RAEE 14 Connect to the COS Supply rca 15 CONTROL PANEL OVERVIEW iii 18 OPERATION ava 20 TOVO OPa O ili ALA ita 20 Preparing nei CUDA OF Tor US ribassi 22 PANG VARIO NE AC iaia lata 23 Set the Temperature Set POI TTT 24 Muting the Audible Temperature Aam ee e eee eee eee 25 Won uesBeonqeg X tr meter 25 Sele DB O EH 26 Muting the Audible CO Alarm e 27 No Gas Supply Alarni NQS ERREUR 27 Temperature Accuracy Verification sise 28 COSACCUFACY erea 30 Launching the Decontamination Cycle inserer 32 Aborting the Decontamination Auto Cycle esse eee e e ee 35 Set the Over Temperature LimMit iii 36 Eaz eE A EL E DETTE t P 37 ACC SO CODE E E E E E EE ES 37 DAN ODO CAD ADIN rn ORA AREE IR E A T E A A T 37 Condensation and the Dew P
10. shelving Set Temperature C 3 The cool down period ends when the temperature reaches 50 C a The door will unlock accompanied by a click b The COzdisplay will show the letters PAS when the abort has concluded 4 Return the SCO6AD to normal operations i DECON CYCLE a Press and hold the Operation Selector Switch Saa b The CO display will return to showing the gas concentration NORMAL OPERATION ka level in the chamber OPERATION SELECTOR SWITCH The amber Decon Cycle indicator will deactivate The green Set CO2 Normal Operation light will illuminate ee Mute M End of procedure LAB 35 Page OPERATION CONTINUED Note Test the OTL system at least once per year for functionality SET THE OVER TEMPERATURE LIMIT The incubator must be operating at your incubation application temperature and must be stable for at least 1 hour prior to setting the OTL Perform the following steps to set up the Over Temperature Limit system for use Set OTL Example 1 Turn the Set Over Temperature Limit control dial clockwise to the maximum position if it is not already set to maximum Turn the Over Temperature Limit control dial counterclockwise until the red Over Temperature Limit Activated light illuminates Slowly turn the dial clockwise until the Over Temperature Limit Activated light turns off Stop turning the control a The Over Temperature Limit is now set approximately 1 C above the curre
11. the OTL system once the incubation chamber has stabilized at your application temperature set point and after you have performed any display verifications or calibrations End of Procedure 24 Page LAB OPERATION CONTINUED MUTING THE AUDIBLE TEMPERATURE ALARM An audible and visual high or low deviation alarm will activate if the incubation chamber temperature deviates by 1 C or more above or below the temperature set point The low deviation audible alarm has a delay of 15 minutes This prevents the low alarm from sounding whenever the doors are opened causing a short drop in temperature 1 To mute an active high or low deviation alarm press and hold either the Up or Down arrow on the Temperature Control panel until the amber Mute LED illuminates and the audible alarm shuts off 2 The audible alarm component will remain muted for the duration of the current Figure 9 High temperature deviation The visual alarm indicator will remain illuminated Alarm Muted 3 Any new deviation of 1 C or greater will reactivate the audible alarm AUTOMATIC DOOR CUTOFF Whenever the incubation chamber outer door is opened the incubator stops the flow of CO into the chamber depowers the heater element and ceases operation of the internal blower fan This limits the amount of CO released into the workspace around the incubator It also prevents the heater from attempting to counteract the continual inflow of cooler ai
12. the temperature readings of the chamber and the reference device still fall outside your protocol after three calibration attempts contact your distributor or Technical Support for assistance End of procedure 46 Page LAB USER MAINTENANCE CONTINUED CALIBRATE THE CO DISPLAY Note Performing a CO display calibration requires a gas reference device Please see the Reference Sensor Devices entry on page 10 for the device requirements CO calibrations are performed to match the incubator CO display to the actual gas concentration in the incubation chamber The actual concentration is supplied by a calibrated reference sensor device Calibrations compensate for drifts in the unit microprocessor controller as well as those caused by the natural material evolution of the IR CO sensor when continually exposed to a heated and humidified atmosphere with elevated CO concentrations Calibrate as often as required by your laboratory or production protocol or regulatory compliance schedule CO Supply The incubator must be powered the CO set point set and the chamber supplied with CO for at least two hours prior to the calibration Temperature Temperature drives gas diffusion in the chamber CO calibrations must be performed with the chamber fully heated and stable at your operational temperature set point A CO display calibration may be performed during a temperature calibration as long as the chamber door is
13. value Wait 1 Hour a Failure to wait until the unit is fully stabilized will result in an inaccurate reading and calibration 7 Compare the reference device reading with the incubator CO display again a Ifthe reference device and the CO display readings are the same or the difference now falls within the range of your protocol the incubator is now calibrated for CO b See next step if the difference still falls outside your protocol range Procedure continued on next page LAB Reference Device Set CO2 CO INJECTING Set C02 CO INJECTING Adjusting to Set Point Sef CO2 CO INJECTING Sef CO2 CO INJECTING 49 Page USER MAINTENANCE CONTINUED CO Calibration Continued Reference Device J Repeat steps 3 7 up to two more times if a difference that still falls outside your protocol range Sef C02 CO INJECTING a Three calibration attempts may be required to successfully calibrate units that are more than 2 out of calibration If the CO readings of the display and the reference device still fall outside your protocol after three calibration attempts contact your distributor or Technical Support for assistance End of procedure 50 Page LAB USER MAINTENANCE CONTINUED Page left blank 5b1 Paae LAB ag UNIT SPECIFICATIONS The SCO6AD High Heat Auto Decontamination Incubator is a 100 120 voltage unit Please refer to
14. your incubator s data plate for individual electrical specifications Technical data specified applies to units with standard equipment at an ambient temperature of 25 C and a voltage fluctuation of 10 The temperatures specified are determined in accordance to factory standard following DIN 12880 respecting the recommended wall clearances of 10 of the height width and depth of the inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times WEIGHT Shipping Net Weight 302 Ibs 137 kg 238 Ibs 108 kg DIMENSIONS By inches Exterior W x D x H Interior W x D x H 28 5 x 30 8 x 39 5 inches 20 2 x 20 x 25 5 inches By centimeters Exterior W x D x H Interior W x D x H 72 4 x 78 2 x 100 4 cm 51 4 x 50 8 x 64 7 cm Access Port Diameter 1 5 inches 3 8 cm CAPACITY Cubic Feet Liters 52 Page LAB UNIT SPECIFICATIONS CONTINUED CO2 Range Accuracy Recovery Time 0 20 PUERUM TEMPERATURE Range Uniformity Stability Ambient 5 C to 50 C 0 25 C at 37 C 0 1 C 37 C POWER Model AC Voltage Amperage Frequency PRESSURE CONVERSION TABLE Conversion table for pressure units Se De ue ee ED ml 9 86923 X 10 3 3 0 2953 X 10 9 86923 2 8959 X 10 9 67841 X 107 2 953 X 10 9 86923 X 1071 2 036 6 80457 X 10 3 9370 X 1072 1 31579 X
15. 10 1 46960 X 10 7 60000 X 102 2 9921 X 10 bi 1 mmHg 1 Torr 1 33322 X 10 1 1 33322 X 1 1inHg 3 3864 h 1 atm 1 01325 X 10 1 01325 X 10 1 03323 1 01325 Figure 20 Pressure Conversion Table LAB 53 Page PARTS LIST COMPONENTS Description Parts Number Description Parts Number Access Port Stopper Humidification Pan 7750565 Brass Tubing Fitting Gas Humidification Pan Copper Tubing Kit Component Antimicrobial Token Power Cord 125 volt 9ft 5 in Door Gasket Inner 2 86m NEMA 5 15P Door Gasket Exterior Sold by the foot Requires 9 feet Shelf Slide Shelf Standard Fuse 12 5 Amp 1250V Type H 5x20mm aa Stainless Steel Shelf SE S 3300520 5121777 HEPA Filter for CO2 Gas i Tubing Black 1 8 OD Line Tubing Kit Component Gas Tubing Kit Component 2800525 8500516 Tubing Clear 5 16 OD T ae TEP ng SIC Gas Tubing Kit Component 8500512 Feet Leveling 54 Page LAB PARTS CONTINUED ORDERING PARTS AND CONSUMABLES If you have the Part Number for an item you may order it directly from Sheldon Manufacturing by calling 1 800 322 4897 extension 3 If you are not certain that you have the correct Part Number or if you need that specific item please contact Sheldon Technical Support for help at 1 800 322 4897 extension 4 or 503 640 3000 Please have the model number and serial number of the incubator ready as
16. A corrected chamber display value pa v b The incubator will now begin heating or passively cooling in order Adjusting to Set Point Walt5 Seconds 10 reach the set point with the corrected display value Set Temperature C HEATING ACTIVATED 6 Allow the incubator to operate undisturbed for at least one 1 hour to stabilize after it has achieved the set point with the corrected bd A 9 Low display value Wait 1 Hour a Failure to wait until the incubation chamber is fully stabilized will result in an inaccurate reading Heference Device 7 Compare the reference device reading with the chamber temperature 4 or display again LL If the reference device and the chamber temperature display reer HEATING ACTIVATED readings are the same or the difference falls within the range of your protocol the incubator is now calibrated for temperature figh a V Low a See the next step if the readings fail to match or fall outside of your protocol range Continued on next page LAB 45 Page USER MAINTENANCE CONTINUED Temperature Calibration Continued Reference Device 374 LI Set Temperature C 8 If a difference still falls outside the acceptable range of your protocol repeat steps 3 7 up to two more times HEATING ACTIVATED a Three calibration attempts may be required to successfully calibrate High a units that are more than 2 C out of calibration Mute Low If
17. A module is 250 Ohms At higher loop resistances the current value will be erroneously low for parameter values near the top of the scale Jack Outputs Parameter Parameter Value at 4mA Parameter Value at 20mA CO2 0 CO2 20 CO2 Figure 16 Data Ports 38 P Q O Je LAB OPERATION CONTINUED CONDENSATION AND THE DEW POINT Relative humidity inside the incubator chamber should never be allowed to exceed 95 Exceeding this thresholds will likely result in condensation possible leaks around the incubator and may cause corrosion damage if allowed to continue for any significant length of time Condensation appears wherever the humidity level in the incubation chamber reaches the dew point The dew point is the level of humidity at which the air cannot hold more water vapor The warmer the air the more water vapor it can hold As the level of humidity rises in an incubation chamber condensation will first appear on surfaces that are cooler than the air temperature Near the dew point condensation will form on any item or exposed surface that is even slightly cooler than the air When the dew point is reached condensation forms on nearly all exposed surfaces Managing condensation primarily depends on either lowering the humidity level or increasing the air temperature in the incubator chamber Note Rising or falling air pressure from weather will adjust the dew point up and down in small increments
18. AINTENANCE CONTINUED HEPA FILTER AND GAS LINES Li Sheldon Manufacturing recommends replacing the external in line gas HEPA filter once per year or more often when the filter is noticeably discolored The filter is directional and must be installed facing in the correct direction The word IN is stamped on the rim of the filter assembly on the side that faces toward the 2800525 gas source N Gas lines should be replaced when cracking brittleness permanent kinking or other signs of damage are present Please see the Parts List on 54 STORAGE OF THE INCUBATOR Perform the following steps if the incubator will be out of use for more than 24 hours to prevent microbiological contamination such as fungus or mold 1 Depower the incubator 2 Disinfect and clean prior to drying if required by your laboratory protocol or if the chamber has been exposed to pathogenic microorganism 3 Use a soft cloth to dry the chamber surfaces DOOR AND ELECTRICAL COMPONENTS Inspect the door latch trim catch and gasket for signs of deterioration every six months Failure to maintain the integrity of the door components shortens the lifespan of the incubator Electrical components do not require maintenance If the SCO6AD fails to operate as specified please contact your Shel Lab dealer or distributor or Shel Lab Technical Support for assistance 42 Page LAB USER MAINTENANCE CONTINUED CALIBRATE THE TEMPERATURE
19. ECEIVING YOUR INCUBATOR When an incubator leaves the factory safe delivery becomes the responsibility of the carrier Damage sustained during transit is not covered by the manufacturing defect warranty When you receive your SCO6ADs inspect it for concealed loss or damage to the interior and exterior If you find damage follow the carrier s procedure for claiming damage or loss INSPECTING THE SHIPMENT Before leaving the factory SCO6ADs are packaged in high quality shipping materials to provide protection from transportation related damage Carefully inspect the shipping carton for damage Report any damage to the carrier service that delivered the incubator If the carton is not damaged open the carton and remove the contents Carefully check all packaging before discarding Save the shipping carton until you are certain that the unit and its accessories function properly Inspect the incubator for damage The orientation photo on the following page can serve as a useful reference The unit should come with an Installation and Operation Manual and a Certificate of Compliance Verify that the correct number of shelves shelf slides leveling feet and other components have been included see the table below for quantities Included Accessories 1 Anti Microbial 4 Leveling Feet 1 Power Cord 1 Stainless Steel 3 Stainless Steel Copper Token Humidity Pan Shelves 5800529 2700506 1800510 999 00015 5121777 6 Stainless Steel 4 Stainless
20. HIGH HEAT DECONTAMINATION CO INCUBATOR SHEL Ci 110 120 Voltage Installation and Operation Manual SCO6AD Previously Designated 3552 SCO6AD HIGH HEAT DECONAMINATION CO INCUBATOR Installation and Operation Manual Part Number Manual 4861712 Revision October 16 2015 This unit requires a NEMA 5 15R wall power receptacle to plug into These units are TUV CUE listed as air jacketed CO Incubators for professional industrial or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa and no flammable volatile or combustible materials are being heated These units have been tested to the following requirements CAN CSA C22 2 No 61010 1 2012 CAN CSA C22 2 No 61010 2 010 2004 Reaffirmed 2014 07 UL 61010 1 2012 05 UL 61010A 2 010 2002 03 EN 61010 1 2010 EN 61010 2 010 2014 Supplemented by UL 61010 2 010 2015 2 Page LAB Page left blank D IA LAB TABLE OF CONTENTS INTRODUCTION a 6 General Safety Considerations inserer 6 Engineering Improvements sss eee eee eee iii 7 cceli cccqe kMMMM 7 RECEIVING YOUR INCUBATOR xxssssssssssesaazaazzzzazezzazio e seeeeenienaneazazzz zizzania arae assa assa 8 WSOC CTIA Eeee eases TETTE 8 Que ee T 9 Recording Data Plate Information inner 10 Reference Sensor Re 10
21. Hours Total f Optional Verify CO Display Accuracy page 30 g Run a decontamination cycle page 32 h Set the Over Temperature Limit page 36 i Load the Chamber page 37 End of Preparation Procedure HUMIDIFYING THE INCUBATOR Fill the humidification pan in the incubation chamber Make sure the pan has been placed on the chamber floor The floor is heated and will help drive evaporation to raise the humidity level to 90 95 relative humidity This helps slow the drying of samples in open breathable containers e The humidification pan must be filled in order for the incubator to achieve its stated temperature uniformity specification e Always place and secure the copper token in the pan to slow the growth of microbiological populations in humidification water supply e Regularly clean and disinfect or decontaminate the pan e Refill as needed and change the water in the pan at least once per week e Use of chemical disinfectants added to the pan may alter the surface tension of the water This may significantly reduce the rate of evaporation and impact the humidity level of the incubator chamber e Never use deionized water to humidify the incubator 23 Page LAB OPERATION CONTINUED SET THE TEMPERATURE SET POINT Perform the steps below to change the set point to the operational temperature you will be using during your incubation application The incubator comes from the factory with a set point of 37 C Note Th
22. NTINUED CO ACCURACY VERIFICATION Note Performing a CO display accuracy verification requires a gas reference device Please see the Reference Sensor Devices entry on page 10 for the device requirements Optional The CO display was calibrated at the factory at a 5 concentration A display accuracy verification may be performed when preparing the incubator for use if required by your laboratory or production protocol The verification procedure compares the chamber CO level as measured by the incubator with the actual level as provided by a calibrated reference device If a difference between the actual and displayed CO concentrations is discovered perform a CO display calibration Please see the Calibrate CO Display procedure on page 44 in the User Maintenance section Temperature A CO display verification may be performed simultaneously with the temperature display verification as long as the chamber door is not opened during either procedure The incubation chamber should be heated to and running at your application temperature as temperature drives gas diffusion in the chamber Humidity The incubator should be allowed to come up to humidity in the chamber prior to performing a CO verification Relative humidity affects CO distribution due to its influence on the chamber atmosphere temperature Probes Connect a CO reference device sample tube to the sample port located on the left side Pe of the incubat
23. Sheldon Manufacturing cannot guarantee that the incubator was not exposed to contaminants during shipping Remove all wrappings and coverings from shelving prior to cleaning and installation Please see the Cleaning and Disinfecting entry on page 40 in the User Maintenance section for information on how to clean and disinfect without damaging the incubator or its components A decontamination cycle will be run as part of the Incubator setup in the Operation section SHELVING INSTALLATION Install the shelving and humidification pan in the in the incubation chamber Always install the copper token in the pan Copper is known to have antimicrobial properties which retards the growth of microorganisms in the pan 1 Install the shelf standard rails a Align the keyhole slot of the standard with the mounting peg on the side of the chamber wall b Mount the shelf standard 2 Install the shelf slides a Insert the shelf slide into the shelf standard using a rocking motion b The shelf slide will sit level when correctly installed 3 Install the shelves a Slide into position 4 Install the humidification pan a Place the copper token in the humidification pan Figure 4 b Secure the token using the clip on the bottom Shelving of the pan Installation c Place the pan on the chamber floor LAB INSTALLATION CONTINUED CONNECT TO THE CO SUPPLY Note Always use medical grade CO Use of non medica
24. Tech Support will need this information to match your unit with its correct part 55 Page LAB ACCESORIES Shel Lab offers the following accessories for the SCO6AD Caster Platform A rolling platform for the SCO6AD Allows for greater ease of transportation and cleaning of floor space Part Number 9000574 CO Cylinder Regulator Part Number 7150509 Compressed Gas Cylinder Switcher Automatic Allows two gas cylinders to be connected to one gas port and automatically switches from the first to the second cylinder when the first is empty Part Number 2002 B Copper Shelf Assembly Three copper shelves Includes six copper shelf slides Copper is known to have antimicrobial properties Part Number 9750582 complete assembly described above PN 5820504 Individual Shelf PN 5820505 Individual Slide Stacking Stand A stacking stand for two SCO6AD incubators Part Number 9000575 56 Page Sheldon Manufacturing Inc P O Box 627 Cornelius Oregon 97113 EMAIL tech Shellab com INTERNET http www Shellab com PHONE 1 800 322 4897 503 640 3000 FAX 503 640 1366
25. all source b Connect the clear tubing to the CO to Chamber port on the back of the Figure 6 Gas incubator Tubing Kit 5 Do not initiate a flow of CO to the incubator at this time End of procedure e Figure 7 CO2 to Chamber LAB 15 Page GRAPHIC SYMBOLS CONTINUED The incubator is provided with multiple graphic symbols on its exterior and internal surfaces These symbols identify hazards and the functions of the adjustable components as well as important notes in the user manual Definition Symbol Indicates that you should consult your user manual for further instructions Indique que l op rateur doit consulter le manuel d utilisation pour y trouver les instructions compl mentaires Indicates Caution hot surface Indique Avertissement symbole de surface chaude Indicates Temperature Rep re temp rature Indicates the Over Temperature Limit system Indique le syst me de d passement de temperature Indicates AC Power Rep re le courant alternatif Indicates I ON and O OFF rep re de la position MARCHE de l interrupteur d alimentation O rep re de la position ARR T de l interrupteur d alimentation Indicates protective earth ground Rep re terre lectrique Indicates UP and DOWN respectively Touches de d placements respectifs vers le HAUT et le BA Indicates Potential Shock Hazard Signale danger lectrique gt o sede Indicates the unit should be recycled Not disposed of in land f
26. alt8 Hous performing the verification The incubator must operate humidified at its verification temperature set point Figure 14 Probe End 2 for at least 1 hour with no fluctuations of 0 1 C or greater in order to be d pem dindaa considered stabilized Failure to wait for stabilization will result in an inaccurate verification If the chamber door is opened during the verification the chamber must be allowed to re stabilize before continuing 28 Page LAB OPERATION CONTINUED Verifying the Temperature Display Accuracy Reference Device 1 Once the incubation chamber has stabilized compare the reference n temperature device and chamber temperature display readings d Li ar a Ifthe readings are the same or if a difference between the two 2 falls within the acceptable range of your protocol the Set Temperature C HEATING ACTIVATED display is accurately showing the chamber air temperature tEATING P High The Temperature Verification procedure is now 7 complete oee step 2 if a difference falls outside the acceptable range of your protocol Heference Device TET 38 1 Set Temperature C HEATING ACTIVATED S 2 If there is an unacceptable difference a display temperature calibration must be performed to match the display to the reference device a Please see page 43 in the User Maintenance section Mute Low End of procedure LAB 29 Page OPERATION CO
27. an be used to adjust the temperature set point or place the display in its temperature calibration mode and then enter a display value correction Red LED alarm indicators marked High and Low illuminate whenever the temperature deviates by 1 C or greater from the current set point The yellow LED marked Mute illuminates whenever an audible deviation alarm is being muted See the Muting the Audible Temperature Alarm entry on page 25 of the Operations section for more information O The green indicator labeled Heating Activated illuminates whenever the temperature control system is heating the incubation chamber 18 Page LAB CONTROL PANEL OVERVIEW CONTINUED CO2 Control and Display Panel Labeled Set CO 96 the display on this panel shows the concentration of CO in the incubation chamber as a percentage of the chamber atmosphere with a range of 0 to 20 During the automatic decontamination cycle this display shows messages announcing which cycle stage the unit is running in as well as a timer countdown while in the two hour heat soak stage The panel can also show the CO set point as well as display adjustments while in gas calibration mode When starting the incubator the display shows LO until the CO sensor registers a concentration in the chamber greater than 0 When initially injecting CO2 into the chamber a few minutes may be required to build up a sufficient concentration to register The UP DOWN a
28. ation cycle that does not require the removal of the CO2 sensor or other electronic components The incubator features a glass viewing door that allows visual inspection of samples without compromising the chamber CO or humidity environment Heating When powered the incubator heats to and maintains the incubation chamber air temperature at the currently programed temperature set point An internal microprocessor stores the temperature set point The microprocessor board is wired to a solid state temperature probe located on the chamber interior right wall When the processor detects that the chamber temperature has dropped below the temperature set point it pulses power to heating elements located in an insulating air jacket space surrounding the chamber on four sides and beneath the chamber floor A fan circulates air within the jacket distributing heat and providing a temperature uniformity superior to direct radiant heating The processor employs proportional integral derivative analytical feedback loop functions when measuring and controlling the chamber air temperature PID controlled heating pulse intensities and lengths are proportional to the difference between the measured chamber temperature and the current set point The frequency of pulses are derived from the rate of change in that difference The integral function slows the rate of pulses when the temperature nears the set point to avoid overshooting The exterior chamber door
29. contamination cycle It does not need to be removed at any point during normal operations including decontamination The incubator automatically ceases injecting CO for the duration of the cycle The cycle will not trip the Over Temperature Limit system LAB OPERATION CONTINUED Decontamination Cycle Continued 7 Take the unit out of normal operations a 8 Launch the a Press and hold the Operation Selector Switch on the Decontamination Cycle display until the amber Decon Cycle indicator begins flashing The green Normal Operation indicator will extinguish decontamination cycle Press and hold the Decontamination Cycle Switch until the amber Decon Cycle and red Decon Activated indicators flash alternatingly The doors will lock with an audible click 9 Ramp up stage a b 10 Soak stage a The Decon Cycle and Decon Activated light will continue to flash alternatingly while the incubator ramps up to 180 C The CO display will show the letters dEC to indicate that the decontamination cycle is running Under normal conditions the ramp up will take 1 5 hours 90 minutes However the presence of additional mass such as water in the humidification pan may extend the ramp up period as more material must be brought to temperature When the incubator chamber air temperature reaches 180 C the Decon Cycle and Decon Activated lights will remain on continuously The CO display
30. e visual example below depicts adjusting the incubator set point from 35 C to a 37 C application temperature Set Temperature Set Point 1 Turn the OTL control clockwise to the maximum if not already set to max a This prevents the over temperature limit system from interfering with setting the set point Set Temperature C 2 Press and release either of the temperature arrows to activate nop tien the temperature set point adjustment mode A a The temperature display will briefly flash the letters SP to indicate the Set Point is about to be displayed b The temperature display will then show the flashing Set Temperature C HEATING ACTIVATED adjustable temperature set point DE Mute Low Note The display will automatically exit the adjustment mode after 5 seconds of inactivity with the last shown set point value saved Set Temperature C HEATING ACTIVATED High 3 Use the Up or the Down arrow keys to adjust the set point to your Mute application temperature T Adjusted Set Point 4 Wait 5 seconds after entering the set point Sef Temperature C a The display will stop flashing the set point is now saved in the mn controller Mute b The chamber will now automatically heat or passively cool to Hi 9 match your set point Heating to Adjusted Set Point Wait 5 Seconds The display will revert to showing the current chamber air temperature See the Set the OTL procedure on page 36 for how to set
31. emperature Display Accuracy Verification procedure on page 28 for the correct introduction and placement of the thermocouple probe 2 Verify that the port stopper is in place in the access port on the outside of the unit 3 Verify that the workspace power supply and the incubator power requirements listed on the unit data plate have been matched a See the Power Source Requirement entry on page 12 4 Attach the power cord that came with the incubator to the power inlet receptacle on the back of the unit 5 Plug the power cord into the workspace supply outlet 6 Place the Power switch in the on position Procedure continued on next page 22 Page LAB OPERATION CONTINUED Preparation Procedure Continued 7 Perform the following procedures in the Operation section to finish preparing the incubator a Humidifying the Incubator page 23 b Set the Temperature Set Point page 24 Allow the incubator to heat undisturbed for 6 hours before continuing C Open the CO supply control or gas regulator so it supplies a flow of 15 20 psi as per the supply description on page 15 Wait 6 Hours d Set the CO Set Point page 26 After setting the set point wait for an additional 2 hours for a CO2 i concentration to establish and stabilize in the chamber and for the Malt 2 Hours unit to finish stabilizing thermally undisturbed with no door openings e Optional Verify Temperature Display Accuracy page 28 E 8
32. ence between the two readings that falls outside the acceptable range of your protocol see the next step Reference Device 2 Adisplay calibration adjustment must be entered to match the incubator CO display to the reference device Ta CO INJECTING 3 Place the display in its CO calibration mode Set CO2 Press and hold both the UP and DOWN Set CO arrow COs INJECTING buttons simultaneously for approximately 5 seconds Hed ns Mute Release the buttons when the display shows the letters CO The display will begin flashing the current CO display value Note If an arrow key is not pressed for 5 seconds the display will cease flashing and store the last displayed value as the new current chamber CO value Procedure continued on next page 48 Page LAB USER MAINTENANCE CONTINUED Calibrate the CO Display Continued 4 Use the Up or Down arrows to adjust the current CO display value until it matches the reference device CO reading 5 After matching the display to the reference device wait 5 seconds a The display will cease flashing and store the corrected display value b The incubator will begin injecting CO or allow the current gas Wait5Seconds Concentration to decay in order to achieve the set point with the corrected display value 6 Allow the incubator to operate undisturbed for at least 1 hour to stabilize after it has achieved the CO set point with the corrected display
33. ence device Set CO2 a Please see page 44 in the User Maintenance section CO INJECTING End of procedure LAB 31 Page OPERATION CONTINUED Note The incubator was run through a decontamination cycle at the factory However the manufacturer cannot guarantee the unit was not been exposed to contaminants during shipping LAUNCHING THE DECONTAMINATION CYCLE Carryout the following steps to launch the auto decontamination cycle iP 2 Note Note 32 Page Remove all samples and equipment Leave shelving inside the chamber if allowed by your laboratory protocol Empty the humidification pan of all water a Failure to empty the pan will extend the ramp up heating and cool down stages of the decontamination cycle This may adversely impact the cycle efficacy Remove the copper token from the humidification pan a Leaving the token in the pan during a decontamination cycle will result in oxidation of the token and possible discoloration or staining of the humidification pan b Manually disinfect the token using disinfectants and cleaning methods appropriate to your laboratory protocol Place the humidification pan back on the chamber floor Close both the internal and external incubator chamber doors a The decontamination cycle will not initiate while the doors are open Procedure continued on next page The infrared CO sensor is protected from exposure to high temperatures during the de
34. has cooled e Do not stack SCO6ADs without use of a stacking rack e Use only approved accessories Do not modify system components Any alterations or modifications to your incubator can be dangerous and void your warranty e Follow all local ordinances in your area regarding the use of this incubator If you have any questions about local requirements please contact the appropriate agencies 6 Page LAB INTRODUCTION CONTINUED ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products As a result engineering changes and improvements are made from time to time Therefore some changes modifications and improvements may not be covered in this manual If your unit s operating characteristics or appearance differs from those described in this manual please contact your Shel Lab dealer or distributor for assistance CONTACTING ASSISTANCE If you are unable to resolve a technical issue with the incubator please contact Sheldon Technical Support Phone hours for Sheldon Technical Support are 6am 4 30pm Pacific Coast Time west coast of the United States UTC 8 Please have the following information ready when calling or emailing Technical Support the model number and the serial number see page 10 EMAIL tech shellab com PHONE 1 800 322 4897 extension 4 or 503 640 3000 FAX 503 640 1366 Sheldon Manufacturing INC P O Box 627 Cornelius OR 97113 USA 7 Page LAB R
35. he Calibrate Temperature Display procedure on page 43 in the User Maintenance section Humidity Perform the verification with the chamber fully humidified The humidity level of the chamber impacts its temperature uniformity 8 hours are required for the unit to achieve and stabilize at its operational relative humidity level of 90 95 from a dry state CO A CO display verification may be performed concurrently with the temperature verification Probes Reference device sensing probes may be introduced through the access port Carefully seat the port stopper over any probe wires or use non stick non marking tape to seal any gaps if there is no room for the stopper Use non stick non marking tape to secure the wires and probe head and to seal any gaps Place the sensor probe head of the temperature reference device as close as possible to the geometric center of the incubation chamber A thermocouple Figure 13 Introducing a sensor probe sleeve may be taped to the shelving as long as the exposed sensor probe through the copper end is 2 inches bcm away from the shelf see Figure 14 An ACCESS pont exposed sensor probe in direct contact with the shelving may experience heat sinking which can result in an inaccurate temperature reading Temperature Stability After introducing and placing the temperature probe allow the incubator to operate undisturbed and humidified for 8 hours for example overnight prior to W
36. ill Indique l appareil doit tre recycl Ne pas jeter dans une d charge Indicates CO Gas Indique gaz CO2 16 Page LAB GRAPHIC SYMBOLS CONTINUED This page left blank 17 Page LAB CONTROL PANEL OVERVIEW Set Over Temp Control f f Set Temperature C Set CO2 HEATING ACTIVATED CO INJECTING C High High A gt DECON CYCLE DECONTAMINATION CYCLE SWITCH Mute Mute DECON ACTIVATED Over y Low D NORMAL OPERATION ka Alarm C OPERATION SELECTOR SWITCH 180 C High Heat Decontamination CO SERIES Sheodon Manufacutring Inc Figure 8 Control Panel Power Switch p The round black main power switch controls all power to the incubator and its systems lis the on position and O the off Over Temperature Limit This graduated dial sets the heating cut off point for the OTL temperature limit system The OTL system prevents unchecked heating of the chamber in the event of a failure of the main digital controller For more details please see the Over Temperature Limit System description in the Theory of Operations page 21 The red Over Temp Activated light illuminates when the Over Temperature Limit system cuts off heating by rerouting power away from the heating elements Temperature Control and Display Labeled Set Temperature C this display shows the current air temperature in the incubation chamber accurate to within 0 1 C The arrow buttons c
37. ing as long as the exposed copper end is 2 inches 5cm away from the shelf see Figure 18 An exposed sensor probe in direct contact with the shelving may experience heat sinking which can result in an inaccurate temperature reading Stability Prior to a calibration the chamber must operate undisturbed and humidified at its application temperature set point for 8 hours in order to stabilize A common practice is to introduce and place the temperature sensor probe in the chamber allow the unit to operate and stabilize overnight and then conduct the calibration the next day Failure to humidify the chamber will result in an inaccurate calibration and display reading The chamber is considered stabilized when it has operated for 1 hour with no fluctuations 0 1 C or greater Failure to wait for stabilization will result in an inaccurate calibration and incubator temperature display reading LAB Figure 17 Introducir 3 a sensor probe through the access port Figure 18 Probe End 2 inches bcm From Shelf Surface Wait 1 Hour 43 Page USER MAINTENANCE CONTINUED Temperature Calibration 1 Once the chamber temperature has stabilized compare the reference device and temperature display readings a Ifthe readings are the same or if a difference between the two 2 falls within the acceptable range of your protocol the display is accurately showing the chamber air temperature The Temperature Calibration pr
38. is self heating to bolster the thermal uniformity and stability of the chamber and to minimize condensation on the glass viewing door Leaving the exterior door open for long durations may adversely impact the temperature performance of the incubation chamber It may also create condensation on the viewing door Door openings should be restricted to the minimum necessary to view or access samples in the chamber The incubator relies on natural heat radiation for cooling With the doors closed the lowest chamber temperature that can be sustained is the ambient room temperature plus the waste heat generated by internal electrical and mechanical operations the room temperature 5 C CO Atmosphere The same microprocessor board that controls the chamber temperature also manages the gas concentration of CO in the chamber atmosphere by operating an internal injection solenoid valve connected to the gas input ports The incubator monitors the chamber CO concentration with an infrared sensor in a recirculation box above the incubation chamber The sensor operates on the principle that a specific frequency set of infrared light is absorbed by CO2 The more CO present in chamber the more of that band of infrared is absorbed The sensor is only sensitive to CO so measurement accuracy is consistent regardless of the presence of other gasses in the incubator 20 Page LAB OPERATION CONTINUED The processor employs proportional integra
39. l derivative analytic feedback loop functions when measuring and controlling the CO2 concentration When the PID are active injection lengths are proportional to the difference between the measured concentration and the set point The frequency of injections is derived from the rate of change in the difference Integrator feedback slows the rate of injection as the concentration approaches the set point which helps prevent overshoots When the chamber concentration is stable CO injections take place in small bursts to correct for deviations less than 0 1 The incubator is not provided with a means to actively remove CO from the chamber atmosphere Humidification Passive humidification is provided by filling the humidification pan included with the unit The pan is then placed on the heated chamber floor Evaporation driven in part by heating raises the relative humidity percentage RH of the chamber A copper token included with the pan helps to significantly slow the growth of microbiological populations in the humidification water supply The incubator must be operated humidified in order to achieve its stated temperature uniformity specification Decontamination Cycle The SCO6AD comes with a user initiated eight hour high heat cycle designed to kill microbiological organisms This automated cycle consists of three stages A 1 5 hour ramp up from room or incubation temperature to 180 C a 2 hour soak at 180 C and a 4 5 hour cool down P
40. l grade CO risks introducing contaminants into the chamber may damage the incubator and will void the manufacturing defect warranty CO2 mab Regulator C02 In Port Figure 5 CO2 Supply Source Connections HEPA Filter The incubator may be connected to either a building CO supply source or a gas supply cylinder tank During normal operations the incubator uses only small quantities of CO to maintain the chamber gas concentration Precise regulation of the gas input flow is vital for the incubator performance If connecting to a supply cylinder always use a two stage CO pressure regulator Be aware that some single stage regulators have 2 gauges Make certain your regulator is a two stage regulator 1 Attach the CO regulator to a medical grade CO cylinder if using a cylinder supply 2 Setthe wall source control or cylinder regulator to 15 20 Pounds per Square Inch psi Do not exceed 25 psi PSI Megapascals Kilopascals Bar 15 20 psi 0 103 0 137 Mpa 103 42 137 89 Kpa 1 03 1 378 bar Please see the Pressure Units Conversion table on page 53Error Bookmark not defined in the Unit Specifications section for the formulas for converting psi into other units of pressure measurement 3 Remove the dust cover from the COzto Chamber port on the back of the incubator 4 Connect the gas tubing to the incubator and regulator or wall source 971 050b a Connect the black tubing to the regulator or w
41. mation on the disinfectants compatible with your cultivation or culturing applications If possible remove all interior accessories shelving and other non attached items from the chamber when disinfecting Disinfect all surfaces in the chamber making sure thoroughly disinfect the corners Exercise care to avoid damaging the temperature sensor probes Gas concentrations from evaporating disinfecting agents can inhibit growth or metabolic symptoms in microbiological sample populations Make sure that chlorines amphyls quaternary ammonias or any other overtly volatile disinfecting agents have been rinsed or otherwise removed from the chamber surfaces prior to placing samples in the chamber MINIMIZING CONTAMINATION EXPOSURE The following are suggestions for minimizing exposure of the incubator chamber to potential contaminants LAB Maintain a high air quality in the laboratory workspaces around the incubator Avoid placing the incubator near sources of air movement such as doors air vents or high traffic routes in the workspace Minimize the number of times the incubator chamber doors are opened during normal operations Placing the unit on a rolling caster platform will facilitate cleaning of the floor around the unit If possible move floor seated units out of the space prior to cleaning the floor there See the Accessories section on page 56 to order a castor platform from Sheldon Manufacturing 41 Page USER M
42. nal flow of cold air such as an air conditioning vent or a door to a cooler hallway or adjacent room Block or divert the air or move the incubator e Does the ambient humidity in the room exceed the stated operating range of 80 relative humidity If so lower the room humidity LAB 39 Page USER MAINTENANCE Warning Prior to any maintenance or cleaning of this unit disconnect the power cord from the power supply Avertissement Avant d effectuer toute maintenance ou entretien de cet appareil d brancher le cordon secteur de la source d alimentation CLEANING AND DISINFECTING If a hazardous material or substance has spilled in the incubator immediately initiate your site s Hazardous Material Spill Containment protocol Contact your local Site Safety Officer and follow instructions per the site policy and procedures The incubator chamber should be cleaned and disinfected prior to first use Periodic cleaning and disinfection are required to prevent microbiological contamination Do not use spray on cleaners or disinfectants These can leak through openings and coat electrical components Do not use cleaners or disinfectants that contain solvents capable of harming paint coatings or stainless steel surfaces Do not use chlorine based bleaches or abrasives these will damage the chamber liner Consult with the manufacturer or their agent if you have any doubts about the compatibility of decontamination or cleaning agen
43. not opened during either procedure Humidity Because humidity impacts CO concentration through its influence on temperature stability and uniformity the CO display should be calibrated with the chamber humidified Stability Figure 19 CO Prior to a calibration the chamber must operate at its CO2 set point for at least 1 hour with Sample Port no fluctuations of 0 1 or greater in order to be considered stabilized Failure to wait for stabilization will result in an inaccurate calibration and incubator display reading Probes Connect a digital CO analyzer sample tube to the sample port located on the left side of the incubator adjacent to the control panel For best results allow the unit to operate undisturbed for 8 hours supplied to achieve temperature and RH stability for example overnight A continual CO supply stream may be introduced a minimum of 2 hours with the incubator otherwise undisturbed prior to performing the calibration 47 Page LAB USER MAINTENANCE CONTINUED Calibrate the CO Display 1 Once the incubation chamber has stabilized compare the gas reference Reference Device device and chamber CO display readings a Ifthe readings are the same or if a difference between the two 2 falls within the acceptable range of your protocol the E display is accurately showing the chamber CO concentration dia The CO calibration procedure is now complete If there is a differ
44. nt chamber temperature Optional You may turn the dial slightly to the left to bracket in closer to the set point temperature This sets the OT Limit nearer to the current chamber temperature Leave the OTL dial set just above the activation point If the OTL sporadically activates after setting it you may turn the dial very slightly to the right clockwise If the OTL continues activating check for ambient sources of heat or cold that may be impacting the unit temperature stability Check if any powered accessories in the chamber are generating heat If you can find no sources of external or internal temperature fluctuations contact Tech Support or your distributor for assistance End of Procedure 36 Page LAB OPERATION CONTINUED LOAD THE INCUBATOR Place items on the shelves inside the incubation chamber as evenly spaced as possible Good spacing allows for maximum air circulation and a high degree of temperature uniformity Leave 1 inch 2 5cm between sample containers and the chamber walls This ts the final step in the Preparing the Incubator procedure ACCESSORY COMPATIBILITY Make sure that any accessory equipment used inside the incubation chamber can safely and effectively operate within your selected range of temperature humidity and CO levels DATA OUTPUT CAPABILITIES The incubator generates data outputs describing temperature and CO as a digital log line once per minute These outputs a
45. ocedure is now complete If a difference falls outside of your protocol range advance to step 2 2 Adisplay calibration adjustment must be entered to match the display to the reference device See next step 3 Place the temperature display in its calibration mode Press and hold both the UP and DOWN temperature arrow buttons simultaneously for approximately 5 seconds Release the buttons when the temperature display shows the letters CO The display will begin flashing the current temperature display value Note If an arrow key is not pressed for five seconds the display will cease flashing and store the last displayed value as the new current chamber temperature value Continued on next page 44 Page Reference Device 3 LU L Set Temperature C HEATING ACTIVATED i A Mute Low Reference Device 38 1 Ma r Set Temperature C HEATING ACTIVATED High Mute Low Set Temperature C HEATING ACTIVATED High Mute Low LAB USER MAINTENANCE CONTINUED Temperature Calibration Continued Reference Device we 36 1 Set Temperature C 4 Use the Up or Down arrows to adjust the current display temperature value until it matches the reference device HEATING ACTIVATED temperature reading n A Low Set Temperature C 5 After correcting for the difference wait 5 seconds HEATING ACTIVATED a The temperature display will cease flashing and store the ud
46. oint a The display will stop flashing and the set point is now saved in wel the controller processor High The chamber will now automatically inject CO2 or allow the e 9 ad current level to decay in order to achieve your set point CO Injecting to achieve l the new set point The display will revert to showing the current chamber il concentration Note The CO display and injections can be set to off when in the set point adjustment mode Hold the down arrow after the blinking set point appears until the display reads OFF The incubator will cease injecting CO2 End of procedure 26 Page LAB OPERATION CONTINUED MUTING THE AUDIBLE CO ALARM Visual high or low deviation indicator alarms will illuminate if the chamber CO level deviates 1 above or below the CO set point An audible alarm sounds immediately for a high deviation The low deviation audible alarm will sound after the visual low indicator alarm has been continually illuminated for 15 minutes This delay prevents the alarm from sounding whenever a door opening creates a short lived drop in gas concentration 1 To mute an alarm press and hold the CO Up or the Down arrow button until the amber Mute LED illuminates 2 he alarm will stay muted for the duration of the current temperature deviation 3 Another deviation of 196 will reactivate the audible alarm Deviation 15 20 CO Start Minutes Minutes Set Point 4 1 o
47. oint eee eee 39 USER MAINTENANCE ccc 40 CANIN and aere ie MR 40 MINIMIZING Contamination EXPOSUIC sese e e e e eee ee 41 PIE AUT AIO GAS INOS MEER uuu 42 Tele Tee re T 42 Door ang Electrical CompOFHBlls iussesuoco inse un sess scethesnnenseeesnendsoancecaiesoeendbaiacesneesentedsnancecasnageendhespseesoeeere 42 Calibrate the Temperature display iii 43 Calibrate the CO Display iii 47 UNIT SPECIFICATIONS e 52 4 Page Weight inner POW TS e n A lic ne Pressure Conversion Table cccccccecececcccccctanacececececsanseeceaecactanaesaeaecacsanausueaenacsanausuncecacsanausueuerscnanacs PARTS CIST bla CORRO I I Ordering Parts and Consumables iii ACCESORIES luca 9 LAB Page INTRODUCTION Thank you for purchasing a Sheldon Manufacturing SCO6AD High Heat Decontamination CO2 Incubator We know that in today s competitive marketplace customers have many choices when it comes to constant temperature equipment We appreciate you choosing ours Our continued reputation as a leading laboratory product manufacturer rests with you We stand behind our products and will be here for you if you need us These incubators are intended for laboratory industrial and educational microbiological applications These incubators are not intended for use in hazardous or household locations
48. or adjacent to the control panel Gas Stability Figure 15 CO Allow the unit to operate undisturbed to run for at least 8 hours for heat and humidity Sample Port stability for example overnight prior to performing the verifications A CO flow to the chamber may be started 2 hours prior to the start of the verification Prior to a verification the chamber must operate at its CO set point for at least 1 hour with no fluctuations of 0 1 or greater in order to be considered stabilized Failure to wait for stabilization will result in an inaccurate verification If the chamber door is opened during the verification the chamber must be allowed to re stabilize before continuing Wait 1 Hour Continued on next page 30 Page LAB OPERATION CONTINUED Verifying the CO Display Accuracy Reference Device 1 Once the chamber has stabilized with no fluctuations of 0 1 or greater compare the gas reference device and chamber CO display readings a Ifthe readings are the same or if a difference between the two 2 falls within the acceptable range of your protocol the Set CO2 display is accurately showing the chamber CO CO INJECTING concentration The CO Verification procedure is now complete oee step 2 if a difference falls outside the acceptable range of your protocol Reference Device 047 0 2 If there is an unacceptable difference a CO calibration must be LE performed to match the display to the refer
49. or remains on continuously The CO display will return to showing the CO concentration in the chamber End of procedure 34 Page Set CO2 CO INJECTING DECONTAMINATION CYCLE SWITCH DECON CYCLE DECON ACTIVATED NORMAL OPERATION 9 OPERATION SELECTOR SWITCH Set Temperature C HEATING ACTIVATED High Mute Set CO2 CO INJECTING DECONTAMINATION CYCLE SWITCH DECON CYCLE P DECON ACTIVATED NORMAL OPERATION 9 OPERATION SELECTOR SWITCH Sef C02 CO INJECTING LAB OPERATION CONTINUED ABORTING THE DECONTAMINATION AUTO CYCLE Press and hold both the Decontamination Cycle Switch and the Normal Operation Switch for approximately 4 seconds The CO display will show the letters Ab to indicate that SCO6AD has successfully aborted the cycle DECONTAMINATION CYCLE SWITCH DECON CYCLE ps The Decon Activated indicator light will extinguish though the DECON ACTIVATED Decon Cycle light will remain illuminated aonne onana ka OPERATION SELECTOR SWITCH 2 The CO display will then show the letters Cdn a This indicates that the incubator is cooling down b The amber Decontamination Cycle and green Normal Operation d nes indicators will flash alternatingly throughout the cool down stage i m DECON ACTIVATED Note The door will remain locked during the cool down stage This is a safety L ka feature to safeguard against injuries caused by exposure to hot chamber surfaces and
50. outside of the incubation chamber When the port is not being used to introduce probes into the chamber the stopper should be installed outside the chamber to obtain the best temperature uniformity and prevent condensation from forming inside the port Never tape over the copper filter around the port The incubator depends on a lt enemies controlled flow of air through the filter to achieve its temperature and CO set Figure 3 Access points while maintaining its targeted relative humidity range Port and Stopper INSTALL INCUBATOR IN LOCATION Install the unit in a workspace location that meets the criteria discussed in the previous entries of the Installation section 1750565 DEIONIZED AND DISTILLED WATER Do not use deionized water to clean the incubator Use of deionized water may corrode metal surfaces and voids the warranty Sheldon Manufacturing recommends the use of distilled water in the resistance range of 50K Ohm cm to 1M Ohm cm or a conductivity range of 20 0 uS cm to 1 0 uS cm for cleaning applications LAB 13 Page INSTALLATION CONTINUED INSTALLATION CLEANING AND DISINFECTION If required by your laboratory protocol clean and manually disinfect the incubator chamber and shelving components Cleaning and disinfecting during installation reduces the risk of contamination The chamber and shelving were cleaned at the factory and a decontamination cycle was run to verify the cycle functionality However
51. r which would cause a significant heat spike once the door is closed Normal CO injections heating and fan operation all resume automatically when the outer door is closed 25 Page LAB OPERATION CONTINUED SET THE CO SET POINT The incubator comes from the factory set to Off Set the CO set point to that of your application The gas supply must continually deliver 15 20 psi while establishing and maintaining a CO2 enriched chamber atmosphere A CO flow to the chamber must be started a minimum of 2 hours prior to the start of a display verification or calibration or prior to loading samples in the chamber The CO display will read LO until enough CO has built up for the sensor to register a concentration greater than 096 Set C02 CO INJECTING 1 Press either the Up or Down arrow button on the CO panel a The display will flash the letters SP for set point b A flashing adjustable CO set point will appear in the display Set CO2 CO INJECTING High Mute Low Note The display will automatically exit the adjustment mode after 5 seconds of inactivity with the last shown set point value saved Initial Set Point Set CO2 2 Use the Up or the Down arrow keys to adjust the set point to i Sr your application CO concentration A 9 Low Note This example represents adjusting the CO set point from off to 9 New Set Point 3 Wait 5 seconds after entering the set p
52. r lower Low Visual Alarm Audible No Gas Indicator Alarm Alarm ngs Illuminates Activates Activates Figure 10 Low Gas Alarm Muted Figure 11 Gas Alarm Timeline No GAS SUPPLY ALARM NGS If the Low Gas deviation indicator is active for longer than 20 minutes a second alarm Set CO2 will activate The letters ngS will appear in the CO display to indicate No Gas Supply EM The alarm will remain active even if the incubator is turned off and turned back on The NGS Alarm will remain on until CO2 is restored to the chamber It may take 2 or more minutes of CO inflow to establish a concentration percentage 96 high enough to deactivate the alarm Figure 12 No Gas Supply 2 Page LAB OPERATION CONTINUED TEMPERATURE ACCURACY VERIFICATION Note Performing a temperature accuracy verification requires a temperature reference device Please see the Reference Sensor Devices entry on page 10 for the device requirements Optional The incubator was calibrated at the factory to 37 C A verification of the display accuracy may be carried out when preparing the incubator for use or when required by your laboratory or production protocol The verification procedure compares the incubator displayed temperature with the actual chamber air temperature as provided by a calibrated reference device If a difference between the actual and displayed temperatures is discovered perform a temperature calibration Please see t
53. re transmitted through a USB style RS232 serial port located on the left side of the incubator A software driver and data logging package for the port can be downloaded from the Shel Lab website To download the software visit the product page of any Shel Lab SCO incubator and click on the large USB bar icon titled USB Software for CO Incubators located approximately halfway down the page http shellab com product sco6ad high heat decontamination co2 incubator 5 9 cu ft USB Style Serial Port Output Output Channel Parameter Example logline output C1 37 0 C3 5 0 A dry contact alarm port communicates any alarm activation instances Continued on next page 3 Page LAB OPERATION CONTINUED Optional Outputs SCO6AD units may be ordered from the factory equipped with a 4 20 milliamp analog output board as a special quote SQ unit The analog 4 20 milliamp outputs can be connected to a building management system BMS or other data monitoring and capture system through the use of two jack ports on the left side of the incubator These ports accept standard audio jacks available from most electronics retailers 1 4 inch 2 pole audio connectors also known as phono jacks Jacks are not included with the incubator Data Monitoring Systems Max Resistance For building management and other data monitoring or logging systems the maximum resistance of the current loop driven by either output from the 4 20m
54. rior to launching a decontamination cycle the humidification pan should be drained and its copper antimicrobial token removed to prevent discoloration of the pan Physical and Data Access A copper filtered access port on the right side of the unit allows sensor and monitoring probes such as thermocouples and humidity meter solid state probes to be inserted and left in the chamber without compromising the CO atmosphere An atmosphere sample port for independently verifying the CO concentration in the chamber is provided on a panel on the left side of the incubator This panel also holds a dry contact port that activates whenever a temperature or CO alarm is triggered A USB style serial port on the same panel outputs temperature and CO levels as a digital logline once per minute Please see the Data Outputs entry on page 37 for more details The Over Temperature Limit System OTL When set the OTL system prevents runaway heating in the event of a main control failure by depowering the heating element whenever the temperature in the incubator chamber exceeds the OTL setting Typically the OTL is set 1 C above the temperature display s set point Because of its nature as a cutoff system and its lack of PID analytics the OTL cannot deliver the same degree of temperature stability and measurement precision as the digital display and controls The OTL oystem should only be used as a means of heating regulation for the incubation chamber until a
55. rrow pad can be used to adjust the CO concentration set point and to mute audible gas deviation alarms The control can also place the CO display in its calibration mode and be used to enter a calibration adjustment Red LED alarm indicators marked HIGH and LOW illuminate whenever CO deviations of 1 or greater from the set point are detected The yellow LED marked MUTE illuminates whenever an audible deviation alarm component is being muted See the Muting the Audible CO Alarm entry on page 27 of the Operations section for more information Marked CO INJECTING this green LED indicator illuminates when the incubator is injecting CO2 Q into the incubation chamber Decontamination Cycle Controls and unlock the door prior to and after the cycle Unlocking door after completing or aborting the cycle restores the system to its normal operations mode The panel is also provided with LED lights that indicate what stage of the cycle or cycle abort the incubator is in This panel is used to initiate or abort the automated decontamination cycle Its controls also lock O 19 Page LAB OPERATION THEORY OF OPERATION The SCO6AD is engineered to provide a constant temperature humidified COz enriched incubation environment The incubator can obtain a stable uniform 0 25 C temperature in its chamber ranging from the ambient room temperature 5 C up to 50 C The unit is also provided with an automated high heat decontamin
56. ts with the parts of the equipment or with material contained in it Warning Never clean the unit with alcohol or flammable cleaners Avertissement Ne jamais nettoyer l appareil l alcool ou avec des nettoyants inflammables Cleaning 1 Remove all non attached incubation chamber components and accessories shelves racks and any additional items if present 2 Clean the chamber interior with a mild soap and water solution including all corners 3 Take special care when cleaning chamber sensor probes located at the rear of the chamber on the back wall 4 Clean all removable accessories 5 Clean and disinfect any attached sample tubing and replace if discoloring is present 6 Rinse the chamber surfaces and shelving with distilled water and wipe dry with a soft cloth Do not use deionized water 40 Page LAB USER MAINTENANCE CONTINUED Disinfecting Disinfect the incubation chamber on a regular basis Perform the following steps to manually disinfect the incubator 1 Turn the unit off Open all doors and carry out your laboratory clinical or production space disinfection protocol Disinfect the incubation chamber using commercially available disinfectants that are non corrosive non abrasive and suitable for use on stainless steel surfaces If disinfecting external surfaces use disinfectants that will not damage painted metal or plastic Contact your local Site Safety Officer for detailed infor
57. will show a countdown starting at 120 minutes The 2 hour heat soak period is required to kill most microbiological organisms Procedure continued on next page LAB DECONTAMINATION CYCLE SWITCH DECON CYCLE DECON ACTIVATED NORMAL OPERATION e OPERATION SELECTOR SWITCH DECONTAMINATION CYCLE SWITCH DECON CYCLE DECON ACTIVATED NORMAL OPERATION ka OPERATION SELECTOR SWITCH DECONTAMINATION CYCLE SWITCH DECON CYCLE DECON ACTIVATED NORMAL OPERATION 9 OPERATION SELECTOR SWITCH Set CO2 CO INJECTING mA q Mute Low DECONTAMINATION CYCLE SWITCH DECON CYCLE ps DECON ACTIVATED NORMAL OPERATION 9 OPERATION SELECTOR SWITCH Set CO2 CO INJECTING 33 Page OPERATION CONTINUED Decontamination Cycle Continued 11 Cooldown stage a When the CO display reaches 0 the display will show the letters CAN This indicates that the cycle has entered the cool down stage The amber Decon Cycle and green Normal Operation indicators will flash alternatingly throughout the cool down 12 The cool down stage ends when the chamber air temperature reaches 50 C a The COzdisplay will show the letters PAS when the cycle has concluded b The door will unlock accompanied by click 13 Restore the incubator to normal operations mode a Press and hold the Operation Selector switch until the amber Decon Cycle light extinguishes and the green Normal Operation indicat
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