Product Manual - Taylor Wharton
Contents
1. LS6000 System Components for the LS6000 AT 5140 1196 AUTO TEND Controller RO8K 9C04 24 VAC Wall Transformer RO6K 8C20 Remote Alarm 6999 9021 24 VAC Solenoid Valve 5140 1193 Sensor Assembly Shelf Rack RO36 9C34 RS30 9C50 RO5K 9C50 RS60 9C50 Necktube Core R036 9C24 RS30 9C16 _ROSK 9CI6 RS60 9C16 Ro33 9c11 na na N _ Ordering Information Order all replacement parts and accessories from your distributor Please include the part and model number of your refrigerator the part and model number quantity and description of each part requested For more information or name of your local distributor contact Taylor Wharton at the address listed below Taylor Wharton 4075 Hamilton Blvd Theodore AL 36582 Phone 251 443 8680 Fax 251 443 2250 7950 8348 ay Taylor Wharton 800 TW TANKS 898 2657 Email cryotanks taylorwharton com www taylorwharton com TW 348 AUTO FILL OPERATION WARNING In order to prevent the relief device on nitrogen refrigerator s from opening when the system is in operation the liquid nitrogen supply system must be protected by a pressure relief device that will open when the pressure at the inlet to the refrigerator s is approximately 22 sig 1 5 bar 152 kPa Never install the supply system pressure relief device into a liquid service line Filling the Refrigerator Initial Fill The LS6000 AT uses the AutoTend controller that comes preset from the factory t2. DESCRIPTION OF FRONT PANEL Key Lock This turns the control On Off Turning the Key to the 3 o clock position provides power to the control while rotating the key 12 o clock position turns the control off Start Fill This button opens the solenoid valve and allows LN to flow into the freezer Stop Fill amp Mute This button closes the solenoid valve and stops the flow of LN into the freezer This button also silences the audible alarm Filling LED Lights green to indicate that the solenoid valve is open LN Level LED Lights red to indicate that the liquid level is ab ve the high level sensor Lights green to indicate that the liquid level is between the low level sensor and the high level sensor Lights yellow to indicate that the liquid level is below the low level sensor Please note The LED will not light if the high level sensor is submerged in LN while the low level sensor is located in gas The only time that this can occur is if the sensors are installed backwards Sensor Fault LED Lights red to indicate that a sensor fault has occurred A sensor fault can be either an open circuit or a short circuit in the sensor assembly Low LN Supply LED Lights red to indicate that the LN supply i i low This is triggered when the liquid level does not reach the high level sensor within 1 hour of opening the solenoid valve O O Remote Alarm 24VAC Power Electrical Supply Connections For LS6000 Changing Li
3. front panel see Figure 5 to the on position The audible alarm may sound during setup silence the alarm by pressing the button labeled MUTE WARNING If the fill fails to stop for any reason quickly close the liquid supply valve to prevent overfilling until the cause of the problem can be determined The unit is now under automatic fill control Liquid will be added by the controller as long as the liquid supply and electrical power are maintained Operating Parameters When materials are immersed in liquid nitrogen they will assume the temperature of the liquid 320 F 196 C When material is stored in the vapor phase of the liquid the liquid nitrogen is still a very cold refrigerant but the unit s interior temperature increases somewhat as product is stored higher above the liquid This temperature differential is not significant in many biological storage applications and is affected by the amount of product stored in the unit the type and size of inventory control system and the liquid level in the unit The liquid level in the unit is determined by the position of the sensor probes in the tube located next to the fill tube These probes are set at installation to maintain a specific liquid level See Figure 4 The cycle repeats when the liquid level drops to the low level sensor over time Sensor probes may be moved to define new high and low levels and these levels may be set independently to vary the liqui
4. NG OR REMOVING RACKS To prevent unnecessary loss of liquid nitrogen and accumulation of ice the necktube core the stopper should remain in the container when the stored material is not being ac cessed When accessing stored material the necktube should be removed as briefly as possible When removing material from the racks withdraw the rack just far enough to remove contents Completely withdrawing the rack will unnecessarily expose the stored material to warm room temperature conditions WARNING Some boxes have liquid drain openings some do not If racks are completely removed from the container liquid nitrogen may remain in the either rack and boxes or simply drain from the bottom When removing racks stop briefly at the necktube to allow liquid to drain completely then handle the rack carefully to prevent personal injury Avoid direct rack contact with bare skin The use of proper personal protective gear is strongly urged cryogenic gloves face shield and gown to protect against splashing When room temperature is added slowly lower the rack into the refrigerator to reduce the boiling of refrigerant and the surge of cold nitrogen gas When inserting the rack tilt the bottom of the rack in the direction of the index ring notch The numbers and colors on the rack handles are a convenient aid to inventory control SECURING CONTENTS The contents of all models may be secured with a seal or lo
5. Operating Instructions SAFETY Before using any cryogenic refrigerator read the Handle with Care booklet provided with the unit It details safety precautions that must be understood before using the equipment If a replacement booklet is needed order publica tion TW 10 Handle with Care from your sup plier Following are a few of the safety precautions described in the Handle with Care booklet Please be sure to read the entire booklet Store and use these containers only in well ventilated areas In a confined area nitrogen gas from these units may cause suffocation by displacing air needed for breathing Install a suitable oxygen monitor Do not touch liquid or cold metal surfaces with your bare skin The liquid nitrogen refrigerant is extremely cold 196 C 320 F Exposure to skin or eyes to liquid cold gas or frosted parts could result in a severe frostbite like injury Because of the extremely low temperature a face shield and gloves must be worn when transferring liquid nitrogen and material into or out of these containers Use only the necktube covers supplied with this unit or a listed replacement part A tight fitting plug or stopper will cause a pressure increase in the container that may damage the container and or cause personal injury OPERATION Filling Adding liquid nitrogen to a warm container may cause splashing and will gener ate a significant volume of nitrogen gas as cold liquid contacts warm re
6. be taken to ensure that the liquid level remains below the bottom of the lid Operating the refrigerator with high liquid levels characteristic of liquid phase storage may result in turbulence during fill cycles Caution must be exercised if the unit lid is opened during a fill and appropri ated safety equipment should always be worn Sensor Positioning for the AutoTend Controller The longer sensor probe orange yellow wires contains the Low Level sensor in a pod The shorter probe red black wires contains the High Level sensor The factory sensor positions will maintain a liquid level between 2 0 in to 4 0 in The dimensions used for the factory sensor installation are shown in Figure 2 Stop Fill A Start Fill 2 0 inches 25 inches _ R Figure 2 Sensor Positioning for the AutoTend LIQUID LEVEL ooo O EVAPORATION PERIOD FILL PERIOD Figure 4 Normal Fill Evaporation Cycle Chart CONTROLLER OPERATION Introduction The AUTO TEND Control System is designed to provide simple reliable liquid level control in your LN freezer It operates on 24 Volts AC and uses a two sensor system to open and close a solenoid valve The liquid level the sensor condition the valve condition and the LN supply condition are indicated by lights on the front panel Installation The AUTO TEND Control System is designed to mount onto your Taylor Wharton Cryogenic refrigerator The components plug into the ba
7. ck of the control panel as follows The Solenoid Valve has a 2 pin connector The sensor assembly has a 4 pin connector These plug into the mates on the back of the control panel The sensor assembly should be installed with the yellow and orange wires at the High Level and the black and red wires at the Low Level These are labeled for easy refer ence The Auto Tend controller should not require additional attention to maintain liquid level if an adequate supply of liquid nitrogen is maintained If your protocol calls for you to top off the cryostorage system at the end of a work day or work week press the Start button The unit will fill to the upper allowable liquid level and stop automatically You may choose to manually stop the fill by pressing the STOP button at anytime during the fill Normal Fill Cycle When the unit is filled and the controller is operating the low level sensor is immersed in liquid nitrogen see Figure 4 It s resistance value is interpreted by the controller as in liquid At the same time the high level sensor is above the liquid pool sending the controller an in gas signal In this condition the control panel will read Normal As liquid nitrogen evaporates the liquid level in the refrigerator drops slowly until the low level sensor is above the liquid and sends a different signal to the controller The controller interprets this condition as low liquid and opens the fill soleno
8. ck through tabs on the edge of the lid opposite the hinge ROUTINE CARE AND MAINTENANCE If ice accumulates inside the necktube a general cleaning of the refrigerator should be scheduled as soon as the stored material can Laboratory Systems V aa L V V ay be conveniently transferred to another refrigerator To clean the unit first remove stored material and then pour out the liquid disposing of it out of doors where the cold liquid will not damage driveways and other surfaces Warm the container by purging it with air even after the container has warmed to room temperature to evaporate any collected moisture When the container is ice free and dry rinse the inner vessel with household bleach Wash the inner vessel with a 40 to 1 ratio of water to laundry detergent solution Rinse and dry inside and out thoroughly before placing the container back into service Do not use sharp instruments to chip ice permanent damage to the container could result DO NOT attempt to fasten any service to the con tainer Welding brazing or piercing of the container in any manner will cause permanent damage Refrigeration depends on the presence of liquid nitrogen in the refrigerator Be sure to maintain correct refrigerant levels to prevent loss of stored material Check liquid levels regularly If high evaporation rates are apparent under normal operating conditions the refrigerator may be losing its vacuum Sweating and the formatio
9. d level differential between fills For adjusting the temperature probes see Changing Liquid Level section in this manual Vapor Phase Storage Vapor phase storage is normally utilized when stored product is unable to withstand liquid nitrogen temperatures or when the storage medium vials ampules etc is not designed for liquid phase storage In a typical vapor phase storage system the liquid level sensors are positioned to maintain the liquid level at or below the top of the spider This positioning allows stored product to be kept at cryogenic temperatures without being exposed to liquid nitrogen reducing the possibility of leakage or cross contamination Care must be taken in the positioning of the level of refrigerant in the event of power outages which may disable the controller for an extended period of time Consid eration must also be given to liquid nitrogen availability and delivery schedules Liquid Phase Storage Liquid phase storage is normally utilized when liquid nitrogen temperatures are required to maintain stored product viability and the storage mediums are adequate for storage in liquid nitrogen In a typical liquid phase storage system the liquid level sensors are positioned to maintain the liquid level at or below the top level of the inventory control system During opera tion the upper levels of the inventory control system will at times become exposed as the liquid level fluctuates Care must
10. e will stay open while the user presses the Start Fill Button but will close when the user releases the button Please Note The maximum time that the valve will stay open when the liquid level is above the High Level sensor is one minute The user can open the valve again by simply releasing and then pressing the Start Fill button again Alarm Conditions An alarm condition occurs when a sensor problem develops or the supply tank runs low on LN When an alarm condition does occur the appropriate light on the front panel flashes and an audible alarm is activated Testing the front panel lights To test all the lights on the control except the Filling LED press the Stop Fill amp Mute button and hold for 8 seconds Testing the Remote Alarm To test the remote alarm press the Stop Fill amp Mute button and hold for 13 seconds 5 additional seconds after testing the lights or unplug the power supply from the wall ED Sensor Assembly 24VAC Solenoid Valve Remote Alarm Jack The remote alarm relay has a set of dry contacts capable of carrying 5 amperes current at 30 volts D C The relay is normal during any alarm condition The remote alarm is triggered 30 minutes after an error condition occurs The remote alarm will be reset when the error condition is cor rected Pins 1 and 2 are closed in normal operating condition while pins 2 and 3 are open in a remote alarm condition See Figure 8
11. es can be con nected or disconnected in seconds Installing the Sensor Probes For procedures for installing the sensor probes refer to the section titled Changing Liquid Level in this section of the manual Removing the Sensor Probes Disconnect the sensor probe lead connection from the back of the controller Remove the fill tube Carefully remove the sensor tube plug from the sensor tube and remove the sensor leads from the plug NOTE Ice or frost in the sensor tube may restrict the movement of the sensor probes in the tube Do not pull excessively on the sensor wiring while attempting to remove sensors It may be necessary to remove the sensor tube from the container and allot it to thaw before the sensors can be removed Taylor Wharton 4075 Hamilton Blvd Theodore AL 36582 Phone 251 443 8680 Fax 251 443 2250 7950 8348 ay Taylor Wharton To install the new sensor probes refer to the procedure Changing Liquid Level Removing the Solenoid Valve Turn off valve from LN Supply and disconnect transfer hose Disconnect only the solenoid valve lead connection from the controller board To remove the solenoid valve loosen the compression fitting that connects the fill tube Unscrew the two 2 mounting screws that hold the solenoid valve to the solenoid bracket Then remove the solenoid valve and its associated plumbing Disconnect the plumbing from the inlet and outlet side of the solenoid valve Install
12. frigerator surfaces Add liquid slowly to minimize these effects Be sure there is adequate ventilation Keep your head clear of the heavy volume of vapor that may be produced It is extremely cold and could cause personal injury WARNING DO NOT OVERFILL Over filling may result in personal injury due to liquid spillage DETERMINING LIQUID LEVEL Liquid level must be checked at regular inter vals refrigeration depends on the pressure of liquid nitrogen The liquid level in the container can be determined with a dipstick Insert the dipstick straight into the container so that it rests on the rack positioning fixture on the bottom of the unit After 5 to 10 seconds withdraw the dipstick and wave it back and forth in the air A frosted section will form representing the depth of the liquid in the container WARNING Never use hollow rods or tubes as dipsticks When a warm tube is inserted into liquid nitrogen liquid will spout from the top of the tube and may cause personal injury The liquid level chart shows volume of liquid nitrogen vs depth for LS Series refrigerators These values are approximate and are based on a standard condition with no stored material in the container With store material the liquid volume will be slightly less than the value of the chart Model Liquid Level Equivalents LS750 1 0 in 2 6 liters 1cm 1 0 liters LS3000 L 4800 LS6000 1 0 in 6 6 liters 1 cm 2 6 liters INSERTI
13. id valve admitting more refrigerant The unit fills slowly the control panel will read LOW when the liquid level is above the low level sensor It will continue to display the green filling light until the high level sensor is immersed in liquid Once the level of the liquid reaches the point of the high level sensor the Solenoid Valve will close Figure 4 illustrates this cycle in graph form where liquid level is plotted against time and display graphics are shown as they appear at key points in the cycle Controller Features Level LN Controllers The controller is designed to maintain the LN level in the unit within a user defined range The LN level will be maintained between the low level sensor and the high level sensor When the liquid level reaches the low level sensor LN will be added to the refrigerator until it reaches the high level sensor Basic Operation 1 Automatic Fill The control will open the solenoid valve automatically when the liquid level falls below the Low Level Sensor It will continue filling until the High Level Sensor is covered by liquid Manual Fill The Start Fill button can be pressed at any time and the solenoid valve will open If the liquid level is between High Level Sensor and the Low Level Sensor the solenoid will stay open until the Stop Fill button is pressed or until the liquid level covers the High Level Sensor If the liquid level is above the High Level Sensor the solenoid valv
14. ing the Solenoid Valve To install a new solenoid valve attach the connecting plumb ing to the inlet and outlet connections of the valve using Teflon tape NOTE Arrow on Solenoid Valve indicates flow direction Position the solenoid valve onto the solenoid valve bracket and tighten the two 2 mounting screws Attach the com pression fitting to the fill tube Reinstall transfer hose open supply vavle and check for leaks Sensor Sensor Tube Plug Sensor Figure 9 AutoTend Sensor Installation 800 TW TANKS 898 2657 Email cryotanks taylorwharton com www taylorwharton com TW 348
15. le should be removed regularly and inspected for ice and frost build up NOTE Ice or frost in the sensor tube may restrict the movement of sensor probes in the tube Do not pull excessively on the sensor wiring while attempting to change sensor position It may be necessary to remove the fill tube and tube from the container and allow it to thaw before the sensors can be repositioned Ice and frost buildup in the fill tube may block the flow of liquid nitrogen into the refrigerator during fill This blockage can result in the liquid level dropping to dangerously low levels and may result in the Low Alarm sensor being activated In addition a fill line blockage may cause the low LN Supply Alarm to be activated If the fill line becomes blocked it must be removed from the refrigerator allowed to thaw to room temperature and purged with dry nitrogen or oil free dry air to remove all traces of moisture before being reinstalled Cleaning your K Series CryoStorage System The vessel must be cleaned and sterilized regardless of the type of stored product prior to return to Taylor Wharton for repair of maintenance Auto Tend Controller Maintenance WARNING Unplug the transformer from the wall before proceeding with any repairs The Auto Tend has been designed for easy setup and maintenance All connectors on the controller are uniquely identified snap on plugs The sensor assembly solenoid valve power remote alarm and data lin
16. n of frost on the outer casing are indications that the vacuum integrity of the refrigerator is not normal All necessary steps should be taken to protect the refrigerator s contents If these condi tions persist contact your supplier or Taylor Wharton s Technical Response Department at 1 800 TW TANKS 898 2657 or email us at cryotanks taylorwharton com for information on how to conduct a normal evaporation rate NER test in the field TRANSPORTATION The LS Series is designed for stationary laboratory use They are not designed for transport service Although these refrigerators are rugged they can be damaged if abused or otherwise mishandled When moving a refrigera tor in the laboratory setting take every precaution to prevent sliding tipping bumping or dropping the unit Use only the roller base designed for the refrigerators All refrigerators must be kept upright WARNING Ventilation MUST BE assured to prevent the displace ment of air and the related suffocation hazard RETURNS Manufacturing defects are covered under the containers limited warranty Evidence of mishandling such as dents on the outer vessel or misalignment of the inner vessel are not manufacturing defects If you would like to return goods to REPLACEMENT PARTS Taylor Wharton for any reason you must first obtain a Material Return Authorization MRA number for tracking purposes Please have a description of your symptoms and the refrigerator s
17. o operate The liquid nitrogen supply pressure at the inlet to the unit should be in the range of 10 psig 0 7 bar 69 kPa to 20 psig 1 4 bar 138 kPa for optimum performance Higher operating pressures will increase transfer losses and create excessive turbulence of the liquid in the unit which can generate false signals to the liquid level controller causing the unit to underrfill In liquid phase storage applications excessive turbulence can cause splashing which could result in personal injury and or damage to the unit If the liquid nitrogen supply pressure at the inlet to the unit rises above the opening pressure of the relief valve on the unit liquid nitrogen will be discharged into surrounding area which can cause rapid and very dangerous depletion of oxygen in the atmosphere Once this pressure relief device has opened and cooled to liquid nitrogen tempera ture it will not reset until it has warmed to near ambient temperature THIS COULD PERMIT THE ENTIRE CON TENTS OF THE LIQUID NITROGEN SUPPLY SYSTEM TO BE DISCHARGED INTO THE IMMEDIATE AREA OF THE REFRIGERATOR S WARNING Maintain adequate ventilation to prevent asphyxiation hazard See Safety Precautions Power Supply Connection Connect the 24 Volt AC power supply to the rear of the cryostorage system then plug the power supply into a 110 120 VAC outlet See Figure 5 for the Electrical Supply Connections Turn on the AutoTend by turning the key on the
18. ote Alarm Connection on Taylor Wharton Freezers Back panelson most Taylor Wharton freezers are equipped with a 3 point electrical socket The socket connects to a control board mounted SPDT single pole double throw relay rated at 10 amps 125 VAC f BF NC 2 No 3 A Switchcraft plug 05GM3M connects to the above socket It is available with leads as Taylor Wharton part R06K 8C20 Approximately 9 of wire extend from the plug The gray wire connects to Pin 1 orange wire to Pin 2 and the purple wire to Pin 3 To connect an AC load such as an alarm light or buzzer connect as shown below AC Line 2 orange AC Line gray For automatic dialers and other alarm systems that are alarmed on either a contact make or break connect as shown below orange orange 2 purple sray Alarm on break Alarm on make MAINTENANCE LS Series CryoStorage Maintenance Defrosting your K Series CryoStorage System All liquid nitrogen storage systems are subject to ice and frost buildup over time Regular preventive maintenance programs should be instituted to remove ice and frost from the sensor and fill tubes and from the refrigerator lid Ice and frost build up in the sensor tube may result in false readings being relayed to the controller from the sensors Ice can form a thermal barrier around a level sensor rendering it insensitive to the temperature differences between vapor and liquid Sensors and thermocoup
19. pass through The sensor tube plug holds the sensors at the position necessary to maintain a specific liquid levels Two different sensor heights are specified by their position within the sensor tube The low and high sensor pods are separately positioned to set the liquid levels at which the controller will start or terminate each fill cycle Insert the sensor leads into the perforated sensor tube to the desired height Mark the sensor leads at the top of the sensor tube Pull the leads out just enough to install the sensor tube plug around the marks on the sensor leads Insert sensor plug securely into the mouth of the tube Perform this operation carefully so the sensor leads are not damaged NOTE The high level sensors must be at least 1 75 in 5 1 cm above the low level sensor pod After repositioning sensors check to be sure the sensor tube is secured to the fill tube and the sensor wires are dressed and clear of rack operation and turn the controller on The controller should fill the refrigerator to the new liquid level After sensors are repositioned the controller should maintain the liquid pool at the new operating level Remote Alarm Connection Relay connections are provided on an external for user installation of a remote alarm circuit see Figure 8 Wiring external power supply and alarm devices must be supplied by the user During an alarm condition contacts 1 amp 2 are closed and contacts 2 amp 3 are open Rem
20. quid Level The liquid level in the unit is determined by the position of the sensor probes in the sensor tube next to the fill tube These probes have been set at installation to maintain a specific liquid level The controller operates a fill cycle that adds liquid at low level fills to a predetermined high level then stops the fill The cycle repeats when liquid drops to the low level over time Sensor probe positions may be changed to define new high and low liquid levels and these levels may be set indepen dently to vary the liquid level differential between fills If a higher liquid level is desired withdraw the sensor tube for a low level the sensors must be moved further into down the sensor tube CAUTION Ice or frost in the sensor tube may restrict movement of sensor probes in the tube Do not pull excessively on sensor wiring while attempting to change sensor position It may be necessary to remove the sensor from the con tainer and allow it to thaw before the sensor can be repositioned Increasing the distance between low and high sensor probes allows greater liquid level fluctuation less frequent filling and reduced fill loses decreasing the distance has the opposite effect To set the liquid level to a different point or to change the level differential the sensors must be repositioned Their position within the sensor tube is held in place by the sensor tube plug which is split to allow the sensor leads to
21. serial number ready Contact your supplier or call Taylor Wharton s Technical Response Department at 1 800 TW TANKS 898 2657 or email us at cryotanks taylorwharton com ACCESSORIES The following accessories are available for LS Series refrigerators e Roller Base with ball bearing swivel casters to provide convenience and portability within a working area where frequent container movement is necessary or desirable P N RO33 8C00 LS750 P N RO5K 8C00 LS3000 LS4800 amp LS6000 e Low Liquid Level Alarm for passive monitoring of refrigerant level This AC powered alarm is available for units that may be used for long term storage where an unusually low liquid could go unnoticed P N RO36 8C390 LS750 P N RS30 8C40 LS3000 P N ROSK 8C26 LS4800 P N RS60 8C26 LS6000 Note Liquid level should be checked regularly Low level alarm not available for LS6000 AT e Liquid Level Measuring Rod that is graduated in both inches and centimeters This accessory is inserted into the cryogenic refrigerant in order to determine the actual liquid level P N RO33 8C11 for all units e Inventory Control Boxes made of cardboard or plastic to hold 2ml vials Can be used to store samples P N N 378247 Cardboard 25 CEM DOK susene LS750 P N N 374180 Plastic 29 CEM DOX cunnan LS750 P N N 374187 92 Plastic 100 CONDOX srasz LS3000 LS4800 amp
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