iTrack Pipette Tracking System
Contents
1. Figure 17 Figure 18 Figure 19 Figure 20 Figure 21 Figure 22 Figure 23 Figure 24 Figure 25 Figure 26 Figure 27 Figure 28 Figure 29 Figure 30 Table of Figures PID CEES COTO Te EE 3 Illumination of pipetted location cc ccccceeeccceecccenscceseceesecceeesseeecseeeees 3 Log file records every Step EE 3 Mack COT ORCINUS oosit E TN 6 CONNECT AIC K eaer E E EEE 6 Bottom view with kickstand esseenesseenssssensssserssssresssserssseressseersssserssseeress 7 DEE AEE EE 7 Adapter Aand C Et e 8 Adapter A and Cholesizes cece cccccceeeccceeccceecccenscceeceeecseeucseeucseeseseuseseuenss 8 Place a 96 Well Plate on Adapter A 9 Place a 96 well ELISA or storage plate on adaptor BR 9 Place a 384 well plate directly in the Detection Region 9 Upper scanning layer for 96 well plates cc eccccesccceeesceeecseeesseeeeees 10 Lower scanning layer for 384 well olates eccceeecceeecceeecseeesseeeees 10 MER 10 Oben E Eege 11 Neie 12 Red light indicates the detection is inactive eee ecccceeeceeeeceeeesseeeeees 12 US BC ONAN C CUS OU caisetcor cacieeneeesevensaaiewuncvaiievosdsaivivssesaiovotorausnadesicuneaiuseeecsien 13 WISE EE 13 kleine 14 The number indicates how many times the well is pipetted 00 15 End the pipetting SOs Le ME 16 384 Well plate user INTER ACE E 16 LOC TC 17 Loe me NUS E 17 LOS TE ee a E 17 VE UE Te E 18 Sottware ele E E 18 ED SS OHS Eege 19 Z2. On the Home page click Log File to display the pipetting records Figure 25 Log File Click specific log files to show the options to open delete or rename the log files P Mnt internal_sd iTracker omn Ronee Bij rename e log_20141219 114934 txt P log_20141218 132221 txt e log_20141218 125112 txt P log_20141218 124111 txt Figure 26 Log file list Each log file includes the time and location of every pipetting step in text format Click icon to go back S a mnt internal_sd iTracker log_20141228 090845 txt 2014 12 28 09 08 45 Start a new pipetting session 2014 12 28 09 09 01 H1 G1 F1 E1 D1 C1 B1 Al 2014 12 28 09 09 04 H G F2 E2 D2 C B2 A 2014 12 28 09 09 13 Pause 2014 12 28 09 09 14 Resume 2014 12 28 09 09 22 H3 G3 F3 E3 D3 C3 BS AJ 2014 12 28 09 09 24 H4 G4 F4 E4 D4 C4 B4 Ad 2014 12 28 09 09 41 H4 G4 F4 E4 D4 C4 B4 Ad 2014 12 28 09 09 45 Undo 2014 12 28 09 09 53 H5 G5 F5 E5 D5 C5 B5 A5 2014 12 28 09 10 00 H6 G6 F6 E6 D6 C6 B6 AG 2014 12 28 09 10 11 Undo 2014 12 28 09 10 16 Redo 2014 12 28 09 10 30 H7 GT F7 ET D7 C7 BY AT 2014 12 28 09 10 38 End pipetting session Figure 27 Log file content 17 www maestrogen com Software Update For best performance please keep the software and firmware up to date Following the steps below to check and update Step 1 Click About button at either 96 well or 384 well interface
3. The system will connect to the Internet automatically if Wi Fi is set up gt Run N Home ak Preference Figure 28 Menu About Step 2 Click Upgrade if the Firmware or App is out of date after checking iTrack Firmware ver 1 1 0 20141205 2 release up to date Track App ver 1 1 0 20141210 out of date Upgrade Figure 29 Software update The URL of the user manual is also listed in the About screen 18 www maestrogen com Troubleshooting If the following problems are encountered please refer to the following suggestions for troubleshooting For further assistance please contact your local customer services 1 iTrack does not work after connecting the tablet via the USB cable a Re connect the USB cable b Re start the iTrack app c Reboot the Tablet d Check if the USB cable is damaged e Check if the USB port of tablet is damaged 2 Track shows the LED and sensor failure indicator x the sensor failure indicator 19 or the LED failure indicator Q co mt aD ch f La E A a e 0 00 0000 0i 0 00 0000 0L 0 00000 00 0 00 0000 0L 0 00 0000 0L LC LC 0000 0000 0 00000 00i P OOO OOO OOO OO Oe OOO OOO OOO OOO Figure 30 LED amp Sensor error a The microplate or tubes may be sitting too high blocking the sensing signal Please choose an appropriate adapter to make sure the top surface of the microplate or tub
4. www maestrogen com About iTrack iTrack a proprietary pipette tracking system is a tablet powered smart lab device that can detect guide and record manual pipetting It helps users avoid pipetting errors and keep a detailed record of each pipetting step for troubleshooting and quality control iTrack uses two layers of sensors to detect the locations of pipette tips The lower layer is for 384 well plates whereas the upper layer is for 96 well plates and Strip tubes Figure 1 Pipette detection zx eme e w Figure 2 Illumination of pipetted location mnt internal_sd iTracker log_20141228 090845 txt 2014 12 28 09 08 45 Start a new pipetting session 2014 12 28 09 09 01 H1 G1 F1 E1 D1 C1 B1 Al 2014 12 28 09 09 04 H2 G2 F2 E2 D2 C2 B2 A2 2014 12 28 09 09 13 Pause 2014 12 28 09 09 14 Resume 2014 12 28 09 09 22 H3 G3 F3 E3 D3 C3 B3 A3 2014 12 28 09 09 24 H4 G4 F4 EA D4 C4 B4 A4 2014 12 28 09 09 41 H4 G4 F4 EA D4 C4 B4 A4 2014 12 28 09 09 45 Undo 2014 12 28 09 09 53 H5 G5 F5 E5 D5 C5 B5 A5 2014 12 28 09 10 00 H6 G6 F6 E6 D6 C6 B6 A6 2014 12 28 09 10 11 Undo 2014 12 28 09 10 16 Redo 2014 12 28 09 10 30 H7 G7 F7 E7 D7 C7 B7 A7 2014 12 28 09 10 38 End pipetting session Figure 3 Log file records every step Patent pending 3 www maestrogen com Technical Support Maestrogen provides technical support for all of its products If you have any questi
5. 0000 0 00 0000 0L 0 00 0000 0L 0 00 0000 0R 0 00 0000 0L 0 00 0000 0L 0 00 0000 0 00 0000 0 00 0000 IQ nmo gt Figure 18 Red light indicates the detection is inactive 4 Help Press Help button will open the latest iTrack manual if it is already downloaded to the tablet A PDF Reader is needed to read the manual The free PDF Reader can be installed from Google Play store 12 www maestrogen com If the latest iTrack manual is not downloaded to the tablet pressing Help will start the downloading process 5 USB connection Je The indicator Ka shows the tablet is connected to the base via USB 9 TO 71nmMwVvV lt OaATDW Dp 0 00 0000 0i 0 00 0000 0L 0 00 0000 0i LC LC F10000 0k LC L CC L CC EE e A Figure 19 USB Connected The indicator AM shows the tablet is not connected to the base Figure 20 USB Disconnected Operation Instruction iTrack can be operated with the tablet s battery or plug in power with included AC adapter The AC adapter supplies 5V DC Avoid direct sunlight fire infrared light and blinking lights nearby to prevent interference 13 www maestrogen com 1 Turn on the tablet Press and hold the power button for 3 5 seconds 2 Open the iTrack app Connect the base with the tablet through the attached USB cable and the micro USB OTG adapter Figure 4 amp 6 After connecting the tablet will start iTrack app automatically The iTrack ap
6. amp adapter Micro USB OTG adapter Ce USB connection activates the App Adaptor Figure 5 Connect iTrack 6 www maestrogen com Kickstand x Kickstand for tilted view Figure 6 Bottom view with kickstand Assembly Control Region E S Figure 7 iTack assembly 7 www maestrogen com Microplate Adapter Selection Plate Type Adapter 384 well full skirt plate No adapter is needed 96 well PCR plate amp Low profile tube strips adapter A 96 well ELISA or storage plate adapter B Full height high profile tube strips adapter C not included adaptor C CCCCCCCCLOAOA ee OO050S099980 SEI 000005900990 SEI 00600 00004598990 ee RRR EE 2 K L d 000000000000 F cocccccccoce 060660000000 0606958806 All the holes are the same size in the adaptor C OG6466666868 06000060606080 but it doesn t in the adaptor A i Ei Figure 9 Adapter A and C hole sizes 8 www maestrogen com 00000000000 FHFF o osos L 00000000000 Figure 10 Place a 96 Well Plate on Adapter A Log Fie WM Ext m Figure 11 Place a 96 well ELISA or storage plate on adaptor B L Loo Fie MENT Figure 12 Place a 384 well plate directly in the Detection Region The purpose of the adapters is to secure microplates and tubes to the correct location and adjust their heights so that they sit just below the detection layers It is import
7. ant to make sure the top surface of the plate or tube does not obstruct the scanning layer The upper scanning layer should be clear of any object other than the pipette during a 96 well detection session The lower scanning layer 9 www maestrogen com should be clear of any object other than the pipette during a 384 well detection session Upper scanning layer Figure 13 Upper scanning layer for 96 well plates Lower scanning layer Figure 14 Lower scanning layer for 384 well plates Buttons and Indicators esch GC CC 0000 000 0L Gleleielelelselek 00 0 0000 0L olol o ol Ce 0 00 0000 0i OOooeoeoce 0 00000 0 0L leese leese ek OO OO OO Od OO Od Od OO Figure 15 Indicators and Buttons 10 www maestrogen com 12 C ore QO QO Menu Refresh Work Status Help USB Connection 1 Menu Touch the Menu Button method b at upper right corner will bring up the menu The menu can also be brought up by swiping from left side of the screen to the right method a gt Run Method b Press the Menu wb Home ff Preference Abou Mitte Swipe from left to right ks NZ NK Figure 16 Open the Menu o Run Start a new pipetting detection session or resume a paused detection session Please make sure the scanning layer is free of any object including pipette when clicking the Run button o Pause Pause detection of the current session Pause shows u
8. de MOES TROGEN iTrack Pipette Tracking System Cat No TK 01 Instruction Manual VH N Version 1_14 www maestrogen com Table of Contents Table OF COMTCIIS occas snasssccaxessacecmasaiecansusasese auasseardusauecmasaiacad dusasesh auasaeaddusGuesnasaiacatausadeanons TREO ROUNO EE 2 ADOUCIR ICK e A E E A E E E AE 3 LANC Re ee GE 4 MET 4 Reg latory Tee EE 4 FOG UCL SCOUT CANONS EE 5 BS UY e A E E 5 Whats INCIU GOO ME 6 CORNE CO era E 6 Wie ele 7 ee TEE 7 Microplate Adapter Selection ccccceccccccccccsscccesceceseccesscceeecseeucsseseceeesseecseeeseeeeseees 8 Buttons and Bio lee 10 E TEE 11 De PRONO ee E desctodasaeensosscuercossacetodadeeceesasaeutt 12 3 Work SU ACU E 12 Ae e Cl EE 12 Dg USB COMM OC d Te E 13 Operation IMS EE 13 1 T ra onthe tableta enesesse S EENE 14 2 Open the VACA e EE 14 2 PLCE BS CFO E 14 A SEPE aa ET e EOE EA 14 S OPED INEMEN U eee ere 14 Be elt A R n DUWO EE 14 7 Select the EE 15 Si SUSE DID SUC E 15 9 End the pipetting S SSION cccccssscccescccecsccevscsecacsceecacecsenecsenavscsucacseeacas 15 10 Change the plate type o oo cccccesccceeecceeecceeecsceescseeceeeeseeeeeseeeseeeeseees 16 Rea TEE 17 SOR ARO hee E E 18 TO IES OO gl EE 19 1 www maestrogen com Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 Figure 13 Figure 14 Figure 15 Figure 16
9. ed multiple times it will show a different color and the number of times it is pipetted o It s preferred to perform the next pipetting step after the tablet shows the previous pipetting location o Make sure the pipette tip only moves up and down when pipetting 2 3 4 5 6 ff 8 9 10 11 12 La 919191919199 9j 010000000 NININININININI N 010000000 PIDI CIDI DISIE 0000000 OOOOOOO 0 00 0000 0 olololololololo 00000000 O 7 THO le fl nu le fl e DW gt Q Q Q Q Q Q Q Q OO Q Q Q Q O O Q O Figure 22 The number indicates how many times the well is pipetted 9 End the pipetting session Click End button to terminate the pipetting session A confirmation window will pop up to confirm A text log file will be saved automatically if the Auto Save Log file option is checked in the Preference The log file records every pipetting step pipetting location and time 15 www maestrogen com Click End Lea Undo La Home at Preference About Sy EH Do you want to end current pipetting session Warning the current pipetting session will be terminated Click Yes Double check message ZX Home Auto saving Ce Preference About Kg Aa Figure 23 End the pipetting session 10 Change the plate type Click Home to return to the Home page and select a different plate type Figure 24 384 Well plate user interface 16 www maestrogen com Log File
10. es is below the scanning layer b Re start the iTrack app before pressing Run to start the pipetting session please make sure the scanning layer is free of any object K l c Press to refresh the detection 3 High error rate of detection K a If encountered two or more errors consecutively press to refresh the 19 www maestrogen com detection Avoid moving the microplate or tubes during the session b Make sure the scanning layer is not blocked by any object when the Run button is pressed Keep the scanning layer free of any object other than the pipette tips during the session c Try the different sensitivity setting d Check if the well adaptor is at the right position e Re plug or change a new Micro USB OTG adaptor f Clear iTrack app cache in the tablet setting 20 www maestrogen com
11. is instrument for purposes other than those for which it is intended or by modifications of the instrument not performed by Maestrogen or an authorized agent 4 www maestrogen com Product Specifications Items Specifications Compatible Pipette Single 8 amp 12 channel fixed space Compatible Tip 0 1 1 000 uL 96 Well Microplate height lt 23 5 mm 384 Well Microplate height lt 11 mm 8 tube 12 tube strips 255mm x 230mm x 54mm 10 x 9 x 2 Setup Compatible Microplate Tube PCR ELISA storage Carefully unpack the iTrack instrument ES Place the instrument on a stable horizontal surface without vibrating devices such as centrifuges or ice makers around Turn on the tablet Press and hold the power button for 3 5 seconds Set up clock network connection and others as needed from the tablet Connect the tablet to the base using the Micro USB OTG adapter Figure 5 Avoid direct sunlight fire infrared light and blinking lights nearby to prevent a Ss interference 7 The instrument uses infrared LED to detect pipetting locations The slits that house sensors layer should be kept clean and clear 8 The screen is protected by a film It can be operated with or without the film 5 www maestrogen com What s included Base Detection unit AC adapter Tablet PC Control unit Micro USB OTG adapter Figure 4 iTrack components Connection AC cord
12. ons about the product use or operation please contact Maestrogen 8275 South Eastern Avenue 200 Las Vegas NV 89123 USA E Mail info maestrogen com Warranty iTrack is warranted against defects in materials and workmanship for 1 year after the purchase If any defects occur in the instrument or accessories during this warranty period Maestrogen will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded 1 Damages caused by improper operation misuse accident or disaster 2 Repair modification or part substitution performed by anyone other than Maestrogen or its authorized agent 3 Use of fittings or spare parts supplied by anyone other than Maestrogen 4 Corrosion caused by solvent or samples For any inquiry or request for repair service contact your local Maestrogen office Inform Maestrogen of the model and serial number of your instrument Regulatory Notice IMPORTANT The iTrack instrument is designed and certified to meet safety standards and EMC regulations Certified products are safe to use when operated in accordance with the instruction manual This instrument should not be modified or altered in any way Alteration of this instrument will 1 Void the manufacturer s warranty 2 Void the safety and EMC certification 3 Create a potential safety hazard Maestrogen is not responsible for any injury or damage caused by the use of th
13. p at the Run button location when the session is active o End End the current detection session Undo Erase the signal light and number generated from last pipetting step as if it didn t happen However the log file still records the last step as well as the Undo action o Redo Restore the signal erased by Undo Home Return to the Home Page It will end the current session if it has not been terminated o Preference e Choose the type of pipette being used Single 8 or 12 channel e Adjust the detection sensitivity if needed The default is optimal for most users and usually do not need to be adjusted Lower sensitivity improves reliability of the detection e The Auto Save Log file is checked by default Each pipetting step will be saved in a log file automatically If this feature is not desirable uncheck the box 1 www maestrogen com Preference ig Detection Mode 8 channel Pipet Auto Save Logfile OK Cancel Figure 17 Preference o About Check software amp firmware update see Software Update 2 Refresh Detection may stop if the microplate is moved during pipetting If iTrack fails to NW detect pipetting after repeated attempts press the Refresh button to resume the detection 3 Work Status o Red The detection is inactive No pipetting will be detected o Green The detection is active 2 ch NO 0 00 0000 0i 0 00 0000 000 000001 0 00
14. p can also be started manually from the tablet To save energy the Wi Fi will be turned off automatically by the iTrack app 3 Place the microplate Place the microplate or tube strips in the detection area with the appropriate adapter see Microplate Adapter Selection if needed Make sure the top surface of the plate tube does not block the detection layer 4 Select plate type Press 96 Well Plate or 384 Well Plate from the Home page Choose 96 Well Plate when using strip tubes Log Fic Ext Figure 21 Home page 5 Open the menu Use one of the two methods see Figure 15 a Swipe from left side of the screen to the right b Click the menu button at upper right corner of the screen 6 Click Run button The Run button can be used to start a new session and resume or paused a current session The Run button cannot be clicked if the tablet is not 14 www maestrogen com connected to the base via USB 7 Select the pipette type Preference screen will pop up Select the pipette type and press OK to start the pipetting session Figure 16 8 Start pipetting o After each pipetting step after the pipette tip leaves the well the detected pipetting location will be shown in light on the tablet screen both underneath the well in the detection area and in the control area The last pipetting location is shown in continuous flash light o If the same well is pipett
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