phm-104 computer-controlled variable speed microliter syringe pump
Contents
1. i Pump Operate Rate ul sec Syringe ul Sum Check Sent Sum Check Rec Exit Select the pump node number displayed on the pump the dose in microliters the rate in microliters per second and the syringe size in microliters Then select Set Pump to send this data to the pump The pump s status will be displayed in the Sum Check Sent and Sum Check Rec display windows If a pump is not responding verify that power is on and all network connections have been made If the pump is responding the Pump Operate button is activated Click Pump Operate to run the pump with the entered settings The pump may be run repeatedly with these settings by clicking Pump Operate again Click Exit to close the window MED PC Controlled Operation Please refer to the MED PC IV User s Manual for a complete guide to installing the MED PC IV software building a valid Hardware configuration with the Hardware Configuration utility and opening and compiling a MSN procedure in the Trans IV utility The MSN procedures utilized by MED PC IV are found by the application in a folder on the PC s hard drive The location by default is C MED PC IV MPC Commands The PUMPSET command sets up all pump parameters for the specific pump and retains them until they are changed or power is turned off The PUMPOP command selects the pump node and turns the pump on It is not necessary to issue a PUMPSET command each time the pump is turned on Allow2. med ASSOCIATES ING PHM 104 OPERATING INSTRUCTIONS Syringe Loading A great deal of care should be used when loading syringes to ensure that proper plunger position and alignment are obtained Proceed as follows e Flush the syringe with distilled water or other suitable solution to provide lubrication in order to avoid damage to the plunger seal during the alignment procedure It is not necessary to keep the syringe filled at all times e Cycle the pump from Flush to Operate so that the is in the preset start position farthest away from the valve e With the plunger fully inserted into the syringe carefully screw the syringe into the valve luer Tighten securely using caution not to strip the threads e Pull the plunger back to the actuator and attach it with the thumbscrew Do not tighten securely as further fine positioning is required e Cycle the actuator by switching the Flush Operate switch to Flush Observe the plungers motion into the syringe barrel The tip of the plunger should travel to about 1 mm of the end of the barrel to ensure proper pumping ability If it does not it should be adjusted by loosening the small set screw on the plunger head with the Allen Key provided Adjust the plunger to the desired position and retighten the set screw Move in small increments to avoid having the plunger hit the end of the barrel Cycle again to verify proper position e Finally monitor the movement of the plunger rod for pr
3. PHM 104 COMPUTER CONTROLLED VARI ABLE SPEED MICROLITER SYRINGE PUMP USERS MANUAL DOC 144 Rev 1 0 Copyright 2007 All Rights Reserved MED Associates Inc P O Box 319 St Albans Vermont 05478 www med associates com MED ASSOCIATES INC PHM 104 SYRINGE PUMP MED ASSOCIATES INC PHM 104 SYRINGE PUMP INTRODUCTION The PHM 104 Computer Controlled Variable Speed Syringe Pump is designed to be used in the MED PC environment It delivers doses ranging from 20 to 80 microliters at rates from 20 to 100 microliters second using a Hamilton 100 microliter syringe When a Hamilton 500 microliter syringe is used the ranges become 25 to 400 microliters at 100 to 500 microliters second INSTALLATION INSTRUCTIONS DIG 729 USB Installation Connect the included A D power adapter to the port on the back of the DIG 729 USB Then use the included USB cable to connect the DIG 729 USB to any available USB port on the computer Follow the steps on the computer to install the necessary drivers This will only be necessary the first time the DIG 729 USB is connected to the computer USB port Figure 1 DIG 729 USB Front DIG 729 USB USB STATUS PWR el med associates inc Figure 2 DIG 729 USB Back INTERFACE _ 6VDC os MED ASSOCIATES INC PHM 104 SYRINGE PUMP Electrical Connections The PHM 104 node numbers are factory set and should be clearly labeled on each pump Connect the female DB 15 port on Pump 1
4. at least one clock tick between the PUMPSET command and the PUMPOP command i e do not place both commands in the same State PUMPSET Syntax PUMPSET P P3 P3 P4 Where P Pump Node value 1 to 8 P2 Dose 20 to 80 uL 100 uL syringe 25 to 400 uL 500 uL syringe P3 Rate 20 to 100 uL sec 100 uL syringe 100 to 500 uL sec 500 uL syringe P4 Syringe Size 100 uL or 500 ul PUMPOP Syntax PUMPOP P1 Where P Pump Node value 1 to 8 Comments P through P may be numbers constants special identifiers or variables Note variables must be expressed using square brackets P 1 not the normal parenthesis used in MEDSTATE NOTATION Examples The following examples are functionally equivalent Timed injection reward is used as a reinforcer for an FR 10 schedule 50 microliters are injected at a rate of 100 microliters per second using a 100 microliter syringe Example A s s l sl 0 01 PUMPSET BOX 50 100 100 gt S2 2 10 R1 PUMPOP BOX gt 2S2 Example B LIST P 0 50 100 100 S S l S1 0 017 Set P 0 PUMPSET S2 10 R1 PUMPOP BOX P 0 P 1 P 2 P 3 gt 82 P 0 gt S2 MED PC J ournal Errors If a syringe pump parameter is improperly specified or a pump is not responding MED PC will flag the error in the journal necessary corrective action Error 1 Pump is not responding 2 Dose must be 20 80 uL or Dose
5. controller Figure 3 to the INTERFACE port of the DIG 729 USB Figure 2 using the included SG 219C cable Figure 3 Back of the PHM 104 Controller FUSE HOLDER TO SYRINGE PUMP TO WALL OUTLET FEMALE DB 15 MALE DB 15 Control additional pumps using SG 219C cables to connect the male DB 15 port on the Pump 1 controller to the female DB 15 port on the Pump 2 controller then the male DB 15 port on the Pump 2 controller to the female DB 15 port on the Pump 3 controller etc The pumps can be daisy chained together in any order however the last unit in the series must have the highest node number Refer to Figure 4 Figure 4 Daisy Chain Diagram FIRST PUMP SECOND PUMP LAST PUMP CONTROLLER DIG 729 USB CONTROLLER CONTROLLER HIGHEST NODE NUMBER Next connect each PHM 104 pump controller to a syringe pump using the 9 pin connectors Refer to Figure 3 The controllers can now be plugged in to an appropriate AC outlet and quickly tested by turning the power on Figure 5 Set the FLUSH OPERATE switch to Flush The plunger actuator should cycle back and forth until the switch is returned to the Operate position The actuator will stop in a preset position At this point pressing the Test button will cause the unit to deliver a 50 microliter dose at 100 microliters per second Figure 5 Front of the PHM 104 Controller Computer Controlled High Speed Micro Liter Syringe Pump Flush ON Operate OFF Power
6. must be 25 400 uL 3 Rate must be 20 100 uL sec or Rate must be 100 500 uL sec 4 Syringe must be 100 or 500 uL Pump halted due to errors Cause The pump specified by PUMPSET did not return the proper checksum within the time required Dose is not set within the range of the syringe specified in the PUMPSET command Rate is not set within the range of the syringe specified in the PUMPSET command Syringe size is not set to 100 or 500 in the PUMPSET command PUMPOP command not allowed due to errors ina previous PUMPSET command The following list describes the errors and the Corrective Action Verify that a pump with the specified node number exists in the system Verify that the pump power is on Check all network cabling Test the unit using MED Test Call MED Associates for further assistance if necessary Verify that the proper syringe size and dose are used Refer to the PUMPSET command description in this manual Verify that the proper syringe size and rate are used Refer to the PUMSET command description in this manual Change to correct size Check the journal for previous errors and refer to the listings above for explanations
7. oper alignment while traveling back and forth If the rod does not move parallel with the barrel loosen the thumbscrew slightly to allow the plunger head to move in the appropriate direction to allow parallel movement Priming the Pump Connect the fluid supply line to the luer that enters the valve perpendicular to the syringe barrel next to the valve power supply wire Connect the output line to the luer opposite the syringe barrel Switch the Flush Operate switch to Flush and cycle the plunger until fluid exits the output line Check for leaks at the luers and bubbles in the lines It may be necessary to remove the plunger from the syringe and force fluid from the supply through the back of the syringe to allow bubbles to escape from the barrel Once the lines are filled and bubble free the test program can be used to verify proper doses and rates MED ASSOCIATES INC PHM 104 SYRINGE PUMP Using MED Test The PHM 104 can be tested using the MED Test software Open the MED Test application and select PHM 104 from the Misc Modules menu as shown in Figure 6 The screen shown in Figure 7 will appear Figure 6 MED Test 2 MED Test Exit Standard Cards SuperPort SmartCtrl MAE Module Window Help Interface Card Ctrl F11 ANL 926 Ctrl F12 ENV 413 Shift F1 ENV 520 Shift F2 PHM 100VSS Shift F3 PHM 104 Shift F4 PHM 106 Shift F5 PHM 15 Shift F6 Figure 7 PHM 104 Test Screen PHM 104 Node Set Pump Dose ul
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