Installing the RFIC-ER Startup Kit for Dionex ICS
Contents
1. Arrow on labels must point upward J N A Figure 6 Installing the ER3a and ER3b Columns in the RFIC ER Compartment Doc 065295 03 10 12 11 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 10 Remove the acorn nut fitting and ferrule from the green tubing connected to the ER3a column inlet The tubing is labeled SRS Regen Out Insert this end of the tubing into the waste drain hole in the RFIC ER compartment see Figure 7 and push the tubing until it emerges from the drain tubing in the component panel see Figure 8 If a column heater is installed you will need to remove it temporarily to avoid blockage of the green tubing NOTE Save the fitting and ferrule You may discard the acorn nut Waste Drain Hole 11 12 Figure 7 Routing the ER3a Column Inlet Tubing Reinstall the fitting and ferrule onto the tubing connected to the inlet of the ER3a column Connect the end of the green tubing labeled SRS Regen Out to the suppressor Regen Out port and route the tubing behind the column holder for neatness see Figure 8 If you removed the column heater in Step 10 reinstall it now 12 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems SRS Regen Out Line Route Regen Out line behind column holder Route Regen Out line behind column holder Drain Tubing from RFIC ER Compartment Figure 8 Connecting the SRS Rege2. 2 2 2 irri nt oa ed bee ner eet 50 13 1 Pump Stops Unexpectedly 0 0 0 0 0000 50 13 2 Unstable Retention Times 0 20 0 000000 51 Doc 065295 03 10 12 Installing the RFIC ER Startup Kit for the Dionex ICS 1100 ICS 1600 1 Introduction Reagent Free Ion Chromatography with Eluent Regeneration RFIC ER is a low cost alternative to RFIC Eluent Generation RFIC EG systems Rather than generating eluent electrolytically from water using an eluent generator with an electrolyte solution RFIC ER uses the recycled eluent mode of operation of the electrolytic suppressor to regenerate the starting eluent The starting eluent for the RFIC ER system is prepared manually as usual After suppression and regeneration the eluent passes through a series of purification columns before being returned to the eluent bottle With the closed loop RFIC ER system the original 4 liters of eluent can be used for up to four weeks of continuous operation The Thermo Scientific Dionex ICS 1100 and ICS 1600 Ion Chromatography Systems are RFIC ER ready when shipped from the factory To complete the installation order the appropriate kit for your application e RFIC ER Anion Startup Kit Dionex ICS 1100 ICS 1600 P N 069570 e RFIC ER Cation Startup Kit Dionex ICS 1100 ICS 1600 P N 069569 Each Startup Kit includes one Installation Kit and one Consumables Kit Together these kits contain all the consumab
3. Setup 0 cece unaarne 24 7 1 RFIC ER System Requirements 000005 24 7 2 Updating Chromeleon 0 0 00 eee ee eee 24 7 3 Configuring the ERC 10 Properties 2 222020200 24 1 4 Configuring the Dionex ICS 1100 ICS 1600 Properties 27 Doc 065295 03 10 12 i Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 10 11 12 13 Confirming the RFIC ER Wellness Settings 28 8 1 Chromeleon 7 Wellness Settings 02005 28 8 2 Chromeleon 6 8 Wellness Settings 22222222000 30 Conditioning the Suppressor 0 0 cee eee eee eee ee 31 RFIC ER System Operating Guidelines 0 00 32 RFIC ER System Maintenance 0 0 cece eee eee eee 34 11 1 Replacing the Eluent 0 00 eee eee eee 34 11 2 Replacing the ERI Column 0000 37 11 3 Replacing the ER2 Column 222202 eeeeeeenen 39 Adding an Anion Concentrator for Eluent Regeneration AC ER 43 12 1 Installing the AC ER Concentrator Column 44 12 2 Configuring the AC ER Concentrator Column Properties 44 12 3 Confirming the RFIC ER Wellness Settings 45 12 4 Configuring the Dionex AS AP Autosampler Optional 46 12 5 Configuring the Dionex AS Autosampler Optional 49 12 6 Operating Guidelines for Concentrator Mode 49 Troubleshooting 4
4. box AS AP Autosampler General Sharing Segments Pump Link Options Relays Inputs Error Levels Injection Mode Butters fm um Autosampler Yalvels Syringe Size 100 in Top Valve Iniect Loop Size fi 0 368 4000 u Bottom Valve Fraction V Temperature Control pot 10 port po 10 port Limits Lower Limit 4 4 0 60 000 C Upper Limit 60 4 0 60 000 C M Automatically Wash after Abort ui 46 Doc 065295 03 10 12 Adding an Anion Concentrator for Eluent Regeneration AC ER 3 Under Injection Mode select Push NOTE The option selected here determines the injection modes available in the Instrument Method Wizard or Program Wizard in the Chromeleon client 4 Click OK Exit the Instrument Configuration program or Server Configuration program When asked whether to save the configuration changes click Save To select the injection mode and syringe speed 1 In Chromeleon 7 Launch the Instrument Method Wizard Click Next gt to go to the Injection Mode page amp Instrument Method Wizard Sampler AS AP Sampler Injection Mode Injection Mode Inject Mode PushFul Actual Recommended 1 N A Ina Loop Overfil 5 000 500 f1 000 10 000 Accept recommended values Da de A ee N E E ae F tice aan In Chromeleon 6 8 Launch the Program Wizard Click Next gt to go to the first Sampler Options page 2 Se
5. controller Controller_2 26 Doc 065295 03 10 12 Chromeleon Setup 7 4 Configuring the Dionex ICS 1100 ICS 1600 Properties 1 In the Instrument Configuration program Chromeleon 7 or Server Configuration program Chromeleon 6 8 right click the Dionex ICS 1100 or Dionex ICS 1600 to open the system Properties dialog box 2 Click the Options tab in the Properties dialog box In this example the system is a Dionex ICS 1600 and the chromatography data system is Chromeleon 7 Dionex ICS 1600 IC System Inject Valve Error Levels Simulation Data TTL Inputs Trend General Options Signals Head Type amp Limits Solvents State Devices MV Degas C Always Off Always On C Cycle On 0 120 sec Off 0 5940 sec Monitor F Column Heater l Valve 2 RFIC ER Link to ERC Controller ERC 10 Concentrator Under Degas verify that Monitor is selected 4 Under Link to ERC Controller select the controller installed in the system 5 Click OK Doc 065295 03 10 12 27 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 8 6 Exit the Instrument Configuration program or Server Configuration program When asked whether to save the configuration changes click Save NOTE Go on to Section 8 to check the RFIC ER Wellness panel settings Confirming the RFIC ER Wellness Settings 8 1 Chromeleon 7 Wellness Settings 1 To start the Chromeleon 7 client click Start gt All
6. do not enter the eluent bottle Analyte ions in the sample can increase the background conductivity of the eluent while counterions can affect the recovery of certain analytes The ER2 traps all analyte ions from the eluent stream allowing the eluent to be regenerated for reuse The anion ER2 contains high capacity microporous anion exchange resin in the bicarbonate form The cation ER2 contains high capacity microporous cation exchange resin in the hydronium form e The ER Controller ERC 10 is a gas monitor built into the Dionex ICS 1100 or Dionex ICS 1600 The ERC 10 tracks the volume of gas exiting the two ER3 Dionex IonPac Catalyzer Columns During the suppression regeneration process water is electrolyzed to hydrogen and oxygen gases In order to maintain the volume of the eluent these gases need to be converted back to water The ER3a column provides a delay that allows the most electroactive by products to decay the ER3b column has a platinum catalyst to complete the breakdown of any remaining electrolytic by products and to reform water from the oxygen and hydrogen gases If the volume of gas exiting the ER3 columns exceeds the predefined safety limit the ERC 10 shuts down the pump e The 4 liter eluent bottle has a dual line cap The outlet line is directed from near the top of the eluent bottle while the inlet line is directed to the bottom of the eluent bottle Thus eluent is pumped from the top of the bottle and returne
7. impaired Operation does not constitute a hazard Messages d avertissement en francais Signale une situation de danger immediat qui si elle n est pas vit e entra nera des blessures graves a mortelles gt DANGER Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entra ner des blessures graves mortelles gt AVERTISSEMENT Doc 065295 03 10 12 Safety Information gt MISE EN GARDE gt ACHTUNG gt WARNUNG gt VORSICHT Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entra ner des blessures mineures a mod r es galement utilis pour signaler une situation ou une pratique qui pourrait gravement endommager l instrument mais qui n entrainera pas de blessures Warnhinweise in Deutsch Bedeutet unmittelbare Gefahr MiBachtung kann zum Tod oder schwerwiegenden Verletzungen f hren Bedeutet eine m gliche Gef hrdung Mi achtung kann zum Tod oder schwerwiegenden Verletzungen f hren Bedeutet eine m gliche Gef hrdung Mi achtung kann zu kleineren oder mittelschweren Verletzungen f hren Wird auch verwendet wenn eine Situation zu schweren Sch den am Ger t f hren kann jedoch keine Verletzungsgefahr besteht Informational messages also appear throughout this manual These are labeled NOTE and are in bold type NOTE NOTES call attention to certain information They alert users to an unexpected resul
8. screen press the Menu button to display the MENU of SCREENS 2 Press 5 to go to the MODULE SETUP MENU 3 Press 5 again to go to the SYSTEM PARAMETERS screen see Figure 21 SYSTEM PARAMETERS WAIT ENABLED METHOD SYRINGE SPEED 4 CUT SEGMENT VOLUME 10 uL SAMPLE MODE CONCENTRATE INJECT PORT VOLUME 43 uL FLUSH SYRINGE SPEED 4 Help Prompt Figure 21 System Parameters Screen 4 Move the cursor to the METHOD SYRINGE SPEED field and select the speed for the type of sample syringe installed For guidelines on selecting the correct syringe speed refer to Section 3 8 of the AS Autosampler Operator s Manual The AS manual is provided on the Thermo Scientific Reference Library DVD P N 053891 5 Move the cursor to the SAMPLE MODE field and select CONCENTRATE 6 To return to the MAIN STATUS screen a Press Menu twice b Press Enter or 1 12 6 Operating Guidelines for Concentrator Mode When operating an RFIC ER system in concentrator mode Chromeleon uses the Concentrator Volume selected in the Instrument Configuration program or Server Configuration program see Section 12 2 to increment the eluent counter and ER2 counter Use the sequence table to set the volume of sample to be loaded onto the AC ER concentrator column Doc 065295 03 10 12 49 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation For complete operating instructions for the AC ER refer to the JonPac UTAC 2 and AC ER Product Manual The manu
9. the eluent level falls below the minimum fill mark the outlet tubing may not reach the fluid level and the pump may lose prime 13 Restart the pump and suppressor The system is now ready for routine operation Replacing the ER2 Column When to replace the ER2 column The RFIC ER Wellness feature in Chromeleon monitors the use of the ER2 column An Audit Trail message will inform you when it is time to replace the column Thermo Scientific recommends replacing the ER2 column when it expires However it is possible to regenerate the column For instructions on replacing the ER2 column see page 41 For instructions on regenerating the ER2 column see page 42 Never operate an ER2 column beyond its expiration this may result in a buildup of sample co ions in the eluent leading to increased background conductivity and reduced peak areas Doc 065295 03 10 12 39 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation You can check the life expectancy of the ER2 column at any time by reviewing the status information on the RFIC ER Wellness panel The example screen shot here is from Chromeleon 7 For details about these parameters see the following table Volume Injected Volume Remaining Chromeleon 7 Remaining Inj Volume Chromeleon 6 8 Injections Made Injections Remaining Chromeleon 7 Remaining Injections Chromeleon 6 8 Expiration Date Chromeleon 7 Reset ER2 ER2 Expiration Informati
10. 1 column has expired and must be replaced The percentage of the ER1 column that is remaining When the Capacity Remaining is 0 the ER1 column has expired and must be replaced The final day of operation for the ER1 column Clicking Reset ER1 resets the ER1 column counters resetting the parameters above to 0 Click Reset ER1 only after replacing the ER1 column Doc 065295 03 10 12 37 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation How to replace the ER1 column 1 2 Check that the pump and suppressor are turned off Remove the old ER1 column from the ER1 ER2 column bracket clip in the RFIC ER compartment Remove the tubing from the inlet and outlet of the old ER1 column Discard the column Remove the column plugs from the new ER1 column In the RFIC ER compartment connect the tubing labeled ER1 IN to the inlet of the new ER1 column Connect the tubing labeled ER1 OUT to the outlet of the new ER1 column Mount the new ER1 column with the inlet end at the top onto the ER3a column using the ER1 ER2 bracket clip Install the ER1 column to the right of the ER3a and ER3b column assembly see Figure 12 Disconnect the tubing labeled InjVIv P from port P 2 on the injection valve Direct the end of this tubing to a small waste container see Figure 13 Set the pump flow rate to 2 0 mL min and begin pumping the operating eluent through the ER1 column for 20 minutes Make sure the suppressor does n
11. 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems 7 Gather together all of the loose tubing in the RFIC ER compartment press the tubing into the clip on the left wall and close the clip see Figure 17 Route tubing through the tubing clip Figure 17 Routing the Tubing Through the Tubing Clip 6 7 Directing the ERC 10 Eluent Line to Waste In Section 6 1 Step 8 you routed the 1 8 in OD PTFE tube labeled To Eluent Bottle In from the ERC 10 to the tubing chase in the upper left corner of the Dionex ICS 1100 or Dionex ICS 1600 rear panel see Figure 2 Now direct the free end of the PTFE tube to a small waste container Doc 065295 03 10 12 21 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 6 8 Testing for Leaks 1 Turn on the pump and suppressor at the appropriate flow rate and current see the following table NOTE The CS16 Cation Separator has a 5 mm ID All other columns listed here have a 4 mm ID Separator Column Recommended Maximum Suppressor Flow Rate Current AS4A SC Anion 2 0 mL min 27 mA Separator AS9 HC Anion 1 0 mL min 45 mA Separator AS12A Anion 1 5 mL min 25 mA Separator AS14 Anion Separator 1 2 mL min 25 mA AS14A Anion 1 0 mL min 43 mA Separator AS22 Anion Separator 1 2 mL min 31 mA AS23 Anion Separator 1 0 mL min 25 mA CS12A Cation 1 0 mL min 59 mA Separator CS16 Cation Separator 1 0 mL min 88 mA 2 Begin operating the system for 3 hours i e wi
12. IC ER Wellness panel will correct themselves when the first injection is made e When injecting samples manually or with a Thermo Scientific Dionex AS DV Autosampler be sure the injection volume specified in the Chromeleon sequence matches the sample loop size IMPORTANT Entering the wrong injection volume in the sequence will impair the accuracy of the eluent and trap column counters on the RFIC ER Wellness panel this will impair proper operation of the RFIC ER system e Itis not necessary to re equilibrate the system unless you replace the eluent or one of the trap columns Because the RFIC ER system remains on continuously the system is always equilibrated and ready for operation Doc 065295 03 10 12 33 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 11 RFIC ER System Maintenance 11 1 Replacing the Eluent When to replace the eluent The RFIC ER Wellness feature in Eluent Expiration Information Chromeleon monitors eluent use and Operation Time 0 the total volume of sample injected Remaining Time 672 into the system When the limit for either of these parameters is reached Volume Injected 0 an Audit Trail message will inform you that it is time to replace the eluent Remaining Inj Volume 20000 Injections Made 0 You can check the life expectancy of Inectone Ronanig 8 the eluent at any time by reviewing the status information on the RFIC ER Wellness panel The example screen shot here is from Chrome
13. IC ER compartment 8 On the RFIC ER Wellness panel under ER2 Expiration Information Chromeleon 7 or ER2 Chromeleon 6 8 click Reset ER2 12 Adding an Anion Concentrator for Eluent Regeneration AC ER The Anion Concentrator for Eluent Regeneration AC ER column P N 072778 is a general purpose extremely low pressure anion exchange concentrator with a low void volume approximately 70 uL and a capacity of 12 0 weq column The AC ER is housed in a 4 x 16 mm column body to reduce the delay volume and maximize the number of injections that can be made between eluent exchanges With an AC ER concentrator column installed in an RFIC ER system each injection adds only 75 uL of sample matrix 70 uL plus 5 uL for connecting tubing into the eluent Thus 266 injections can be performed before the eluent needs to be replaced regardless of the amount of sample loaded onto the AC ER Also by using a matrix elimination step with the AC ER column in place Doc 065295 03 10 12 43 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation solvents can be directly injected onto the AC ER and then flushed off before injection into the RFIC ER system The AC ER column brings a host of new applications within range of RFIC ER systems including analysis of bromate to 5 ppb in drinking water and analysis of common anions in ethanol Do not inject ethanol or other solvents directly into an RFIC ER system Before injection load solvents onto th
14. Installing the RFIC ER Startup Kit for Dionex ICS 1100 and ICS 1600 lon Chromatography Systems Document No 065295 Revision 03 October 2012 2012 Thermo Fisher Scientific Inc All rights reserved Chromeleon IonPac and SRS are registered trademarks of Thermo Fisher Scientific Inc in the United States PEEK is a trademark of Victrex PLC All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in n
15. Programs gt Chromeleon 7 gt Chromeleon 7 The Chromeleon Console including the ePanel Set for the Dionex ICS 1100 or Dionex ICS 1600 appears On the Dionex ICS 1100 or Dionex ICS 1600 ePanel under ERC 10 Controller click More Details to view the RFIC ER Wellness panel see Figure 19 Check the following RFIC ER Wellness panel settings a Verify that the Chemistry Type matches the column chemistry you plan to run b Under ER1 Expiration Information ER2 Expiration Information and Eluent Expiration Information verify that the counters are set to zero If a counter is set to a value other than zero Click the corresponding Reset button c Check that the current status displayed in the upper left corner is Connected d Under ERC 10 Controller verify that the Mode is either Operation or Power up e Click the Reboot ERC 10 button If a new or freshly regenerated SRS 300 Suppressor is installed in the RFIC ER system go on to Section 9 to condition the suppressor 28 Doc 065295 03 10 12 Confirming the RFIC ER Wellness Settings If the installed suppressor is not new or freshly regenerated this completes the RFIC ER installation procedure Before starting operation review the operating guidelines in Section 10 Gamer sconsa Connected Chemistry Type Anion_Other Suppressor Information Status Off Type None Current m 0 Condition Suppressor ER1 Expiration Information Capacity C
16. S 1600 IC System Dostmann IC5 2000 IC System HP Polymer Labotaiodes ICS 2100 IC System Rheodyne 2 IC5 900 IC System Shodex Valco Varian In the Chromeleon 6 8 Server Configuration program Right click the timebase that contains the RFIC ER system and select Add Device The Add device to timebase dialog box appears In the Manufacturers list box select Dionex IC Integrated Systems Doc 065295 03 10 12 25 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 5 Inthe Modules Chromeleon 7 or Devices Chromeleon 6 8 list box select ERC 10 Controller 6 Click OK to add the ERC 10 to the instrument or timebase The ERC 10 Properties dialog box appears Dionex ERC 10 Controller General Error Levels Device Name Controller Mode Live Module Serial No 081 20490 v Moduleware version Download C Simulated Use Live mode to control a physical module connected to the server Virtual mode can be used to demonstrate the software support for the module and allows one to create methods and panels without having a physical module attached to the server 7 On the General tab make sure that Live mode is selected 8 If more than one ERC 10 is connected to the server select the correct ERC 10 from the Module Serial No list The serial number is on the ERC 10 label 9 Click OK NOTE Iftwo ERC 10 controllers are to be placed in the same instrument or timebase rename the second
17. Volume Injected 0 Remaining Inj volume 130000 ERC 10 Controller Connected W Condition Suppressor Mode OBERE Gas volume 0 00 mi Reboot ERC 10 Reset Gas Volume Figure 20 Example Chromeleon 6 8 RFIC ER Wellness Panel Remaining Injections Reset EC Injections Made 0 Remaining Injections 0 Reset ER2 4 Check the following Wellness panel settings a Verify that the Chemistry Type matches the column chemistry you plan to run b Under ER1 column ER2 column and Eluent verify that the counters are set to zero If a counter is set to a value other than zero click the corresponding Reset button c Under ERC 10 Controller verify that the Connected check box is selected and that the Mode is either Operation or Power up 30 Doc 065295 03 10 12 Conditioning the Suppressor d Click the Reboot ERC 10 button If a new or freshly regenerated SRS 300 Suppressor is installed in the RFIC ER system go on to Section 9 to condition the suppressor If the installed suppressor is not new or freshly regenerated this completes the RFIC ER installation procedure Before starting operation review the operating guidelines in Section 10 9 Conditioning the Suppressor A new or freshly regenerated SRS 300 Suppressor must be conditioned for 16 hours before it is ready for operation in the recycled eluent mode During this conditioning procedure the SRS 300 screens and membranes are equilibrated into the state requi
18. al is provided on the Thermo Scientific Reference Library DVD P N 053891 13 Troubleshooting 13 1 Pump Stops Unexpectedly The ERC 10 electronics monitor the volume of hydrogen gas in the eluent stream If the safety limit is exceeded the ERC 10 shuts down the pump and Chromeleon displays the following message in the Audit Trail Pump stopped because the total gas volume has exceeded the limit To return to normal operation 1 Ifthe RFIC ER Wellness panel is not already displayed display it now For instructions see Section 8 1 Chromeleon 7 or Section 8 2 Chromeleon 6 8 2 Check the controls under ERC 10 Controller The example screen shot here is from Chromeleon 7 If the Total Gas Volume ERC 10 Controller Chromeleon 7 or Gas Volume Mode Operation Chromeleon 6 8 reading is 0 60 mL or higher or if the Mode is Gas Volume Error it Total Gas Volume 0 60 indicates that the volume of gas Gas in Flow 100 exceeds the allowable limit Reset Gas Yolume Click the Reset Gas Volume button to reset the Total Gas Volume or Gas Volume and return the Mode to Operation 3 Restart the pump and suppressor and monitor the gas volume reading If it begins to climb toward 0 60 mL click Reboot ERC 10 This disables the gas monitor for 3 hours 50 Doc 065295 03 10 12 Troubleshooting 4 After 3 hours check the RFIC ER Wellness panel again If the Total Gas Volume or Gas Volume reading is sti
19. as installed during initial installation of the Dionex ICS 1100 or Dionex ICS 1600 go directly to Step 3 now to add the ERC 10 to an instrument Chromeleon 7 or a timebase Chromeleon 6 8 1 The ERC 10 is connected to a USB connector inside the Dionex ICS 1100 or Dionex ICS 1600 When the IC system is connected to the PC Microsoft Windows will immediately detect the ERC 10 and launch the Found New Hardware Wizard Follow the instructions in 24 Doc 065295 03 10 12 Chromeleon Setup the wizard to install the ERC 10 driver During installation select the following options e If asked whether Windows can connect to Windows update to search for software select No not this time e Accept the default option Install the software automatically and click Next gt It is not necessary to insert a hardware installation CD ROM When the ERC 10 driver has been installed click Finish In the Chromeleon 7 Instrument Configuration program Right click the instrument that contains the RFIC ER system and select Add Module The Add module to instrument dialog box see below appears 3 Add module to instrument Instrument System_1 Manufacturers Dionex IC ICS 3000 Systems IC ICS 5000 Systems IC Modules HPLC UltiMate 3000 HPLC Summit Systems co z HPLC Autopurification Systems IC5 1000 IC System HPLC Gynkotek Systems 2 ICS 1100 IC System HPLC Modules Generic ICS 1500 IC System C2 1 l t Agilent IC
20. bing Clip Figure 4 RFIC ER Compartment Tubing Clip assembly anion assembly P N 068202 cation assembly P N 067803 in the RFIC ER Installation Kit see Figure 5 Tubing is connected to the ER3a column inlet and from the ER3a column outlet to the ER3b column inlet Do not remove this tubing or loosen the fittings nm Figure 5 ER3a ER3b Column Assembly 10 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems 7 Remove the plug from the ER3b column outlet Connect the short 1 8 in OD PTFE polytetrafluoroethylene tube from the left side of the ERC 10 in the RFIC ER compartment to the ER3b column outlet 8 Remove the acorn nut fitting and ferrule from the long 1 8 in OD PTFE tube connected to the right side of the ERC 10 The tubing is labeled To Eluent Bottle In Feed this tube through the chase in the upper right corner of the RFIC ER compartment until it emerges from the tubing chase in the upper left corner of the rear panel NOTE Save the fitting and ferrule you will use them in Section 6 7 You may discard the acorn nut 9 Note the arrow on the ER3a and ER3b column labels Install the columns with the arrows pointing upward on the mounting clips located on the ERC 10 Install the ER3a column on the right side of the compartment and the ER3b column on the left side see Figure 6 NOTE The arrow on the column label indicates the direction of flow
21. d in Section 6 1 Step 8 on the end of the long 1 8 in OD PTFE tube labeled To Eluent Bottle In Connect this end of the tube to the fitting and coupler on the Eluent In line to the eluent bottle Add eluent up to the maximum fill mark on the eluent bottle see Figure 9 Doc 065295 03 10 12 23 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 7 Chromeleon Setup 7 1 7 2 7 3 RFIC ER System Requirements The RFIC ER system consumables have limited life spans and require periodic replacement or regeneration The Chromeleon RFIC ER Wellness feature tracks the usage of the consumables and notifies you when they approach the end of their useful life spans When Chromeleon is disconnected from the Dionex ICS 1100 or Dionex ICS 1600 the IC system firmware monitors the consumables usage and stores the tracking information in its permanent memory In order to operate in the RFIC ER mode you need an ERC 10 standard on all Dionex ICS 1100 and Dionex ICS 1600 instruments as well as the appropriate software version e Chromeleon 7 DUOb or later e Chromeleon 6 8 SR6a or later Chromeleon automatically activates the RFIC ER features in the firmware when it detects the presence of an ERC 10 Updating Chromeleon Check the Chromeleon release installed on the PC If necessary see Section 7 1 run Chromeleon Setup to install the software update Configuring the ERC 10 Properties NOTE If the ERC 10 driver w
22. d to the bottom Two gas vents in the eluent bottle cap ensure that any gases produced by the suppressor are safely vented to the atmosphere The eluent bottle and cap provided in the RFIC ER Installation Kit are designed specifically for use with RFIC ER systems Do not operate the system with any other eluent bottle or cap CAUTION La bouteille d luant et son bouchon fournis dans le kit d installation du systeme RFIC ER sont concus sp cifiquement pour les systemes RFIC ER N utilisez aucun autre type de bouteille ou de bouchon avec MiSEENGARDE le systeme RFIC ER 6 Doc 065295 03 10 12 Unpacking and Inspection Die Eluentenflasche und der Deckel aus dem RFIC ER Installationskit sind speziell f r die Verwendung mit einem RFIC ER System bestimmt Verwenden Sie mit diesem System keine anderen VORSICHT Eluentenflaschen oder Deckel 4 Unpacking and Inspection Unpack the shipping containers and verify that all items listed on the packing list are on hand The RFIC ER Anion Startup Kit Dionex ICS 1100 ICS 1600 P N 069570 consists of e RFIC ER Anion Installation Kit Dionex ICS 1100 ICS 1600 P N 069568 e RFIC ER Anion Consumables Kit P N 067791 The RFIC ER Cation Startup Kit Dionex ICS 1100 ICS 1600 P N 069569 consists of e RFIC ER Cation Installation Kit Dionex ICS 1100 ICS 1600 P N 069567 e RFIC ER Cation Consumables Kit P N 067792 Each Installation Kit contains all non consumable items required f
23. dium bicarbonate solution through the ER2 column Use either the Trap Column Suppressor Cleanup Kit P N 059659 or an accessory pump at 5 0 mL min Direct the column effluent to a waste container Pump 40 mL of deionized water through the ER2 column Use one of the following the Trap Column Suppressor Cleanup Kit P N 059659 an accessory pump at 5 0 mL min or a syringe Direct the column effluent to a waste container Go on to Step 6 42 Doc 065295 03 10 12 Adding an Anion Concentrator for Eluent Regeneration AC ER 5 Ifthe ER2 column is a cation column follow these steps a Prepare fresh 0 4 M methanesulfonic acid solution Use either reagent grade methanesulfonic acid or undiluted Dionex CS12A Eluent Concentrate 0 4 Molar P N 057562 b Pump 200 mL of 0 4 M methanesulfonic acid solution through the ER2 column Use the Trap Column Suppressor Cleanup Kit P N 059659 or an accessory pump at 5 0 mL min Direct the column effluent to a waste container c Pump 40 mL of deionized water through the ER2 column Use one of the following the Trap Column Suppressor Cleanup Kit P N 059659 an accessory pump at 5 0 mL min or a syringe Direct the column effluent to a waste container d Goon to Step 6 6 Reconnect the tubing labeled ER2 IN to the inlet of the ER2 column Reconnect the tubing labeled ER2 OUT to the outlet of the ER2 column 7 Mount the ER2 column on the ER1 ER2 column bracket clip in the RF
24. e AC ER column and perform a matrix elimination step to remove the solvent matrix 12 1 Installing the AC ER Concentrator Column 1 2 Turn off the pump and suppressor Remove the old injection loop or concentrator column from the system injection valve Cut two short about 10 cm or 4 in lengths of 0 25 mm 0 010 in ID black tubing Tubing not provided Connect a length of tubing to both the inlet and outlet ports of the AC ER column Connect the inlet of the AC ER column to port 4 of the injection valve Check that the Thermo Scientific Dionex AS AP Autosampler or AS Autosampler is connected to port 3 of the injection valve Connect the outlet of the AC ER column to port 1 of the injection valve Check that the waste line is connected to port 2 of the injection valve 12 2 Configuring the AC ER Concentrator Column Properties 1 Check that data acquisition on all instruments Chromeleon 7 or timebases Chromeleon 6 8 is turned off In the Instrument Configuration program Chromeleon 7 or Server Configuration program Chromeleon 6 8 right click the Dionex ICS 1100 or Dionex ICS 1600 to open the system Properties dialog box 44 Doc 065295 03 10 12 Adding an Anion Concentrator for Eluent Regeneration AC ER 3 Click the Options tab in the Properties dialog box In this example the system is a Dionex ICS 1600 and the chromatography data system is Chromeleon 7 Dionex ICS 1600 IC System Inj
25. ect Valve ErorLevels Simulation Data TTLinputs Trend General Options Signals Head Type amp Limits Solvents State Devices V Degas C Always Off C Always On C Cycle On 0 120 sec Off 0 5940 sec Monitor M Column Heater T Valve 2 RFIC ER Link to ERC Controller ERC 10 Volume 75 10 1000 pL 4 Select the Concentrator check box In the Volume field enter 75 This is the void volume of the AC ER column 70 uL plus 5 uL for connecting tubing 6 Click OK 7 Exit the Instrument Configuration program or Server Configuration program When asked whether to save the configuration changes click Save 12 3 Confirming the RFIC ER Wellness Settings 1 Onthe RFIC ER Wellness panel Chromeleon 7 see Figure 19 Chromeleon 6 8 see Figure 20 verify that the Connected check box Doc 065295 03 10 12 45 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation is selected The check box is either in the upper left corner Chromeleon 7 or under ERC 10 Controller Chromeleon 6 8 Under ERC 10 Controller verify that the Mode is either Power up or Operation 12 4 Configuring the Dionex AS AP Autosampler Optional To select the configuration injection mode 1 In the Instrument Configuration program Chromeleon 7 or Server Configuration program Chromeleon 6 8 right click the AS AP to open the autosampler Properties dialog box 2 Click the Options tab in the Properties dialog
26. ed before the eluent expires Clicking Reset EC resets the eluent counter resetting the Operation Time and Remaining Inj Volume to zero Click Reset EC only after replacing the eluent How to replace the eluent 1 Check that the pump and suppressor are turned off 2 Disconnect the Eluent Bottle Out tubing from the pump 3 Disconnect the tubing from the ERC 10 labeled To Eluent Bottle In from the Eluent Bottle In line at the connection near the eluent bottle 4 Remove the cap from the RFIC ER eluent bottle and dispose of the expired eluent IMPORTANT Always neutralize methanesulfonic acid eluents before disposal 5 Prepare 4 liters of the eluent required for the application Always use ASTM Type I 18 megohm cm filtered deionized water when preparing eluent 6 Add eluent up to the maximum fill mark on the bottle see Figure 9 IMPORTANT For optimal RFIC ER system operation do not reuse expired eluent 7 On the RFIC ER Wellness panel under Eluent Expiration Information Chromeleon 7 or Eluent Chromeleon 6 8 click the Reset EC button This resets the Operation Time to zero and resets the Remaining Time to 672 hours Doc 065295 03 10 12 35 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 10 Reconnect the pump inlet line to the Eluent Bottle Out tubing Direct the ERC 10 outlet tubing to a waste container Prime the pump If you need instructions refer to Chapter 5 of the operator s ma
27. et m ssen die vom elektrolytischen Supressor erzeugten Wasserstoff und Sauerstoffgase ber diese ffnungen in die Atmosph re gelangen k nnen 4 Push the Eluent Bottle Out tubing down so that the tubing label is against the cap and then connect the tubing to the pump inlet line 5 Prime the pump 6 Make sure the suppressor has been hydrated as instructed in the SRS 300 QuickStart Suppressor manuals are provided on the Thermo Scientific Reference Library DVD P N 053891 6 3 Plumbing the ER1 Column for Conditioning 1 Disconnect the tubing labeled TO INJ VALVE IN P from port P 2 on the injection valve Locate the tubing coupler assembly labeled PUMP OUT in the lower left tubing chase of the component panel Connect the tubing just removed from the injection valve to the tubing coupler assembly see Figure 11 Connect TO INJ VALVE IN P tubing to tubing assembly using PUMP OUT coupler Figure 11 Connecting the ER1 Column for Conditioning Doc 065295 03 10 12 15 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 3 Locate the ER1 column in the RFIC ER Consumables Kit 4 Inthe RFIC ER compartment remove the coupler connecting the two black tubes ER1 IN and ER1 OUT Connect the tubing labeled ER1 IN to the ER1 column inlet Connect the tubing labeled ER1 OUT to the ER1 column outlet 5 Mount the ER1 column with the inlet end at the top onto the ER3a column using one of the
28. he liquid flow path through an RFIC ER system Injection Valve PD gt serene ER2 Eluent ER Bottle Controller 1 57 mm 0 062 in OD Tubing 3 18 mm 0 125 in OD Tubing Figure 1 RFIC ER System Fluid Schematic The key to RFIC ER is operating the suppressor in the recycled eluent mode During the suppression operation the suppressor exchanges the counterion of the eluent and analyte for hydronium in anion systems or hydroxide in cation systems during the regeneration operation the counterion is returned to the eluent and the excess hydronium or hydroxide is neutralized Thus the ion balance is maintained throughout the entire suppression regeneration process e The ER1 is a Thermo Scientific Dionex IonPac Eluent Purification Column anion column AEP ER1 cation column CEP ER1 The ER1 is a mixed bed column that includes matrix trap material The ER1 traps any contaminant species that may be liberated from the separator column or suppressor or introduced by the sample thus ensuring the purity of the regenerated eluent The ER1 has a secondary purpose of trapping the matrix counterions introduced by the sample Doc 065295 03 10 12 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation e The ER2 is a Dionex IonPac Analyte Trap Column anion column AAT ER2 cation column CAT ER2 When a sample is injected into the system it is important that the ions introduced in the sample
29. le and non consumable items required to operate an RFIC ER system nonstop for 6 to 12 months NOTE The SRS 300 Suppressor is the only suppressor compatible with RFIC ER NOTE For information about installing an AC ER Anion Concentrator Column in the RFIC ER system and operating in concentrator mode see Section 12 Doc 065295 03 10 12 1 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 2 Safety Information The ER Controller ERC 10 is an integral element of the RFIC ER system The ERC 10 electronics monitor the volume of electrolysis gases such as hydrogen and oxygen in the eluent stream If the predefined safety limit is exceeded the ERC 10 shuts down the pump to prevent the buildup of gases in the eluent bottle 2 1 Safety Messages This manual contains warnings and precautionary statements that when followed properly can prevent personal injury and or damage to the ERC 10 Safety messages appear in boldtype and are accompanied by icons as shown below Indicates an imminently hazardous situation which if not avoided will result in death or serious injury gt DANGER Indicates a potentially hazardous situation which if not avoided may result in death or serious injury gt WARNING Indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury gt CAUTION Indicates that the function or process of the instrument may be IIMPORTANT
30. lect the Inject Mode option 3 Click Next gt to go to the General Settings page Chromeleon 7 or the second Sampler Options page Chromeleon 6 8 Doc 065295 03 10 12 47 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation amp amp Instrument Method Wizard Sampler AS AP Sampler General Settings General Settings Buffer Wash Wash Volume Wash Speed Draw Speed Draw Delay Dispense Speed Dispense Delay Waste Speed Wash Dispense Speed Sample Height pga tattle aga ee ne ee Actual Recommended 11 000 9 200 750 _ fo 10 0 150 5 0 20 7 0 20 5 0 20 20 gt 20 hoo lt r 20 0 20 0 2 000 G 2 000 Accept recommended values 1 000 10 000 0 0 5000 0 pl 0 1 33 0 ws 0 1 33 0 pl s 0 0 300 0 s 0 1 33 0 ws 0 0 200 0 s 0 1 33 0 ws 0 1 33 0 pl s 0 000 30 000 mm The page lists speed recommendations that are based on the selected injection mode syringe size and loop size In most cases you can click Accept recommended values to accept the default settings To change the speed for an option enter a new value in the corresponding Actual box For a description of each option click the Help button on the page 48 Doc 065295 03 10 12 Adding an Anion Concentrator for Eluent Regeneration AC ER 12 5 Configuring the Dionex AS Autosampler Optional 1 From the MAIN STATUS
31. leon 7 For details about these parameters see the following table Operation Time The number of hours the eluent has been in use Remaining Time The number of hours until the eluent expires After 672 hours 28 days the eluent will expire and must be replaced Note This time limitation is independent of pump operation the eluent will expire after 672 hours 28 days even if the system is not operated during this time Volume Injected The volume of sample injected since the last eluent change When the Volume Injected is 20 000 uL the eluent has expired and must be replaced Note Repeated sample injections cause eluent dilution Operating the system with diluted eluent will cause excessive retention time drift for details see Section 10 Remaining Inj The volume of sample that can be injected before the Volume eluent expires Resetting the eluent counter resets the Remaining Inj Volume to zero also When you start the next injection the Remaining Inj Volume will be updated to the correct value 34 Doc 065295 03 10 12 RFIC ER System Maintenance Injections Made Injections Remaining Chromeleon 7 Remaining Injections Chromeleon 6 8 Reset EC To ensure the accuracy of the Remaining Inj Volume be sure to enter the correct injection volume in the Inj Vol column of the sequence table The number of sample injections since the last eluent change The number of samples that can be inject
32. ll increasing and the Mode is Gas Volume Error shut down the system For troubleshooting assistance contact Technical Support for Dionex products In the U S call 1 800 346 6390 Outside the U S call the nearest Thermo Fisher Scientific office 13 2 Unstable Retention Times Unstable retention times may indicate that the vacuum degas assembly is not operating in Monitor mode To select the correct degas mode 1 In the Instrument Configuration program or Server Configuration program right click the Dionex ICS 1100 or Dionex ICS 1600 to open the system Properties dialog box Click the Options tab in the Properties dialog box In this example the system is a Dionex ICS 1600 and the chromatography data system is Chromeleon 7 Dionex ICS 1600 IC System Inject Valve Error Levels Simulation Data TTL Inputs Trend General Options Signals Head Type amp Limits Solvents State Devices M Degas C Always Off C Always On Cycle On 0 120 sec Off 0 5940 sec IV Column Heater me all A er art ade eee ee oe 3 Under Degas select Monitor 4 Click OK Doc 065295 03 10 12 51 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 5 Exit the Instrument Configuration program or Server Configuration program When asked whether to save the configuration changes click Save 52 Doc 065295 03 10 12
33. n Out Tubing Dionex ICS 1100 Shown Doc 065295 03 10 12 13 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 6 2 CAUTION Installing the Eluent Bottle 1 Prepare 4 liters of the eluent required for the application Always use ASTM Type I 18 megohm cm filtered deionized water when preparing eluent 2 Note the minimum and maximum fill marks on the eluent bottle see Figure 9 Add fresh eluent up to the maximum fill mark Figure 9 Eluent Bottle Fill Marks If the eluent level falls below the minimum fill mark the outlet tubing may not reach the fluid level and the pump may lose prime 3 Screw the cap onto the eluent bottle Check that the two gas vents are seated in the cap and are free of obstructions see Figure 10 Figure 10 Gas Vents in Eluent Bottle Cap Do not block the gas vents in the eluent bottle cap If the ERC 10 malfunctions the gas vents must be able to discharge any hydrogen and oxygen gases produced by the electrolytic suppressor to the atmosphere N obturez pas les vents du bouchon de la bouteille d eluant En cas de d faillance de PERC 10 les vents doivent pouvoir vacuer vers l atmosph re l hydrog ne et l oxygene produits par le suppresseur lectrolytique 14 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems VORSICHT Blockieren Sie keinesfalls die ffnungen im Deckel der Eluentenflasche Wenn der ERC 10 nicht korrekt arbeit
34. ntenance How to replace the ER2 column 1 2 Check that the pump and suppressor are turned off Remove the old ER2 column from the ER1 ER2 column bracket clip in the RFIC ER compartment Remove the tubing from the inlet and outlet of the old ER2 column Discard the column Remove the column plugs from the new ER2 column In the RFIC ER compartment connect the tubing labeled ER2 IN to the inlet of the new ER2 column Connect the tubing labeled ER2 OUT to the outlet of the new ER2 column Mount the new ER2 column with the inlet end at the top onto the ER3b column using the ER1 ER2 bracket clip Install the ER2 column to the left of the ER3a and ER3b column assembly see Figure 16 Select the standard flow rate and current settings for the application Turn on the pump and suppressor and begin pumping the operating eluent through the ER2 column for 30 minutes If the pump stops running and Chromeleon displays the Pump stopped because the total gas volume has exceeded the limit message in the Audit Trail follow these steps a On the RFIC ER Wellness panel under ERC 10 Controller click Reset Gas Volume Chromeleon 7 or Gas Volume Reset Chromeleon 6 8 This resets the gas volume to 0 00 mL b Restart the pump and suppressor and monitor the Total Gas Volume Chromeleon 7 or Gas Volume Chromeleon 6 8 reading If the value begins to climb toward 0 60 mL click Reboot ERC 10 This disables the gas monitor f
35. nual for the Dionex ICS 1100 or Dionex ICS 1600 After priming the pump check that the eluent level is above the minimum fill mark on the eluent bottle see Figure 9 If necessary add more eluent to the bottle If the eluent level falls below the minimum fill mark the outlet tubing IMPORTANT _ may not reach the fluid level and the pump may lose prime 11 Select the standard flow rate and current settings for the application Operate the system for 30 minutes to flush the expired eluent from the system If the pump stops running and Chromeleon displays the Pump stopped because the total gas volume has exceeded the limit message in the Audit Trail follow the appropriate steps below a On the RFIC ER Wellness panel under ERC 10 Controller click Reset Gas Volume Chromeleon 7 or Gas Volume Reset Chromeleon 6 8 This resets the gas volume to 0 00 mL b Restart the pump and suppressor and monitor the Total Gas Volume Chromeleon 7 or Gas Volume Chromeleon 6 8 reading If the value begins to climb toward 0 60 mL click Reboot ERC 10 This disables the gas monitor for 3 hours c If after 3 hours the gas volume reading is still increasing shut down the system For troubleshooting assistance contact Technical Support for Dionex products In the U S call 1 800 346 6390 Outside the U S call the nearest Thermo Fisher Scientific office IMPORTANT Do not operate the system with the gas monitor deactivated for an e
36. o way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Revision history Revision 01 released March 2009 initial release of kit Revision 02 released August 2011 Thermo Fisher Scientific transition Revision 03 released October 2012 new part numbers assigned For Research Use Only Not for use in diagnostic procedures Contents 1 IntrodUctiON sii esc wed Java 4 00 el aeleelen vbevdewdas 1 2 Safety Information 0 0 cece eee eens 2 2 1 Safety Messages 0 0 cece eee eee ee 2 2 2 BRC TO Labels sies 4 ta 2A Ob ee len 3 2 3 ERC 10 Specifications 2 22 2202 eee eee eee 4 3 Functional Description 00 0 eee eee nennen 5 4 Unpacking and Inspection 0 0 eee eee eee 7 5 Procedure Overview cece eee iiss tiasaan enep eee 8 6 Initial Setup for Dionex ICS 1100 ICS 1600 Systems 8 6 1 Installing the ER3a and ER3b Columns 8 6 2 Installing the Eluent Bottle 0 000004 14 6 3 Plumbing the ER1 Column for Conditioning 15 6 4 Conditioning the ERI Column 00 0 17 6 5 Completing the ER1 Column Connections 18 6 6 Installing the ER2 Column 0 00000005 19 6 7 Directing the ERC 10 Eluent Line to Waste 21 6 8 Testing for Leaks 0 eee cece 22 7 Chromeleon
37. on 00 Volume Injected 0 Volume Remaining 130000 Injections Made 0 Injections Remaining 0 Expiration Date 6 27 2012 The total sample volume injected since the ER2 column was installed When the Volume Injected is 120 000 uL the ER2 column has expired The volume of sample that can be injected before the sample volume limit 120 000 uL is reached When the Volume Remaining or Remaining Inj Volume is 0 uL the ER2 column has expired Resetting the ER2 counter resets the Volume Remaining or Remaining Inj Volume to zero also When you start the next injection the Volume Remaining or Remaining Inj Volume will be updated to the correct value To ensure the accuracy of the Volume Remaining or Remaining Inj Volume be sure to enter the correct injection volume in the Inj Vol column of the sequence table The total number of sample injections since the ER2 column was installed The number of sample injections that can be performed before the sample volume limit 120 000 uL is reached The Injections Remaining or Remaining Injections value is based on the volume of the last injected sample if no sample has been injected yet the reading is zero The final day of operation for the ER2 column Clicking Reset ER2 resets the ER2 column counters resetting Volume Injected and Injections Made to zero Click Reset ER2 only after replacing or regenerating the ER2 column 40 Doc 065295 03 10 12 RFIC ER System Mai
38. onsumed 0 00 Capacity Remaining 100 00 Expiration Date 6 27 2012 Reset ER1 ER2 Expiration Information Volume Injected 0 Volume Remaining Injections Made 0 Injections Remaining 0 Expiration Date 6 27 2012 Reset ER2 OAR ERC 10 Controller Mode Operation Reboot ERC 10 Total Gas Volume Gas in Flow Reset Gas Yolume Eluent Expiration Information Operation Time 0 Remaining Time 672 Volume Injected 0 Remaining Inj Yolume 20000 Injections Made 0 Injections Remaining 0 Figure 19 Example Chromeleon 7 RFIC ER Wellness Panel Doc 065295 03 10 12 29 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 8 2 Chromeleon 6 8 Wellness Settings 1 To start the Chromeleon 6 8 client click Start gt All Programs gt Chromeleon gt Chromeleon The main window and Browser appear 2 Select View gt Default Panel Tabset to display the panel tabset for the Dionex ICS 1100 or Dionex ICS 1600 3 Click the RFIC ER Wellness tab on the panel tabset to view the RFIC ER Wellness panel see Figure 20 ICS 1600 2 RFIC ER Wellness Sequence Control Status Chemistry Type anion_Other 7 ER1 column Eluent Remaining Operation Time koun Remaining Time Capacity Consumed 0 00 Remaining Time 672 hours Capacity Remaining 100 00 Yolume Injected 0 Remaining Inj Volume Reset ER1 Remaining Inj volume 20000 ER2 column Injections Made 0 f Remaining Inj Volume
39. or 3 hours c If after 3 hours the gas volume reading is still increasing shut down the system For troubleshooting assistance contact Technical Support for Dionex products In the U S call 1 800 346 6390 Outside the U S call the nearest Dionex Thermo Fisher Scientific office Doc 065295 03 10 12 41 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation IMPORTANT When the gas monitor is deactivated do not operate the system for an extended period of time 8 9 10 After rinsing the ER2 column for 30 minutes turn off the pump Under ER2 Column on the RFIC ER Wellness panel click Reset ER2 Add eluent up to the maximum fill mark on the eluent bottle see Figure 9 If the eluent level falls below the minimum fill mark the outlet tubing imPorTANT P may not reach the fluid level and the pump may lose prime 11 Restart the pump and suppressor The system is now ready for routine operation How to regenerate the ER2 column 1 2 Check that the pump and suppressor are turned off Remove the ER2 column from the ER1 ER2 column bracket clip in the RFIC ER compartment Disconnect the tubing from the inlet and outlet of the ER2 column If the ER2 column is an anion column follow these steps a Prepare fresh 0 5 M sodium bicarbonate solution Use either reagent grade sodium bicarbonate or undiluted Dionex Sodium Bicarbonate Concentrate 0 5 Molar P N 037163 Pump 200 mL of 0 5 M so
40. or an RFIC ER system including the ER1 and ER2 mounting clips ER3a and ER3b columns and eluent bottle assembly Each Consumables Kit contains four ER1 columns and one ER2 column Substitution of non Dionex Thermo Fisher Scientific parts may impair IMPORTANT R b system performance and void the product warranty For details refer to the warranty statement in the Dionex Terms and Conditions Doc 065295 03 10 12 7 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 5 Procedure Overview These are the main steps in the RFIC ER installation procedure Install the ER3a and ER3b columns Install the eluent bottle Plumb the ER1 column for conditioning Condition the ER1 column Complete the ER1 column tubing connections Install the ER2 column Test the system for leaks Complete the tubing connections Update the Thermo Scientific Dionex Chromeleon 7 or Chromeleon 6 8 Chromatography Data System software if necessary Configure device properties in the Instrument Configuration program Chromeleon 7 or Server Configuration program Chromeleon 6 8 For a new or freshly regenerated SRS 300 only Condition the SRS 300 for 16 hours 6 Initial Setup for Dionex ICS 1100 ICS 1600 Systems To ensure that the eluent returned to the eluent bottle is identical in composition to the starting eluent several RFIC ER system components must be equilibrated before initial operation This section describes the temporary connections requi
41. ot activate during this time If the pump stops running and Chromeleon displays the Pump stopped because the total gas volume has exceeded the limit message in the Audit Trail follow the appropriate steps below a On the RFIC ER Wellness panel under ERC 10 Controller click Reset Gas Volume Chromeleon 7 or Gas Volume Reset Chromeleon 6 8 This resets the gas volume to 0 00 mL b Restart the pump and suppressor and monitor the Total Gas Volume Chromeleon 7 or Gas Volume Chromeleon 6 8 reading If the value begins to climb toward 0 60 mL click Reboot ERC 10 This disables the gas monitor for 3 hours c If after 3 hours the gas volume reading is still increasing shut down the system For troubleshooting assistance contact Technical Support for Dionex products In the U S call 1 800 38 Doc 065295 03 10 12 RFIC ER System Maintenance 346 6390 Outside the U S call the nearest Thermo Fisher Scientific office When the gas monitor is deactivated do not operate the system for an extended period of time 9 After rinsing the ER1 column for 20 minutes turn off the pump 10 Remove the tubing labeled InjVIv P from the waste container Connect the tubing to port P 2 on the injection valve 11 On the RFIC ER Wellness panel under ER1 Expiration Information Chromeleon 7 or ER1 Chromeleon 6 8 click Reset ER1 12 Add eluent up to the maximum fill mark on the eluent bottle see Figure 9 If
42. red for equilibration 6 1 Installing the ER3a and ER3b Columns 1 Verify that the Dionex ICS 1100 or Dionex ICS 1600 is set up as directed in the operator s manual The manuals are included on the Thermo Scientific Reference Library DVD P N 053891 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems 2 Remove the ERC 10 from the instrument Chromeleon 7 or timebase Chromeleon 6 8 that contains the Dionex ICS 1100 or Dionex ICS 1600 Do not remove the IC system from the instrument or timebase During the initial setup the pump in the Dionex ICS 1100 or Dionex ICS 1600 is used to condition the ER1 column and test the system for leaks In order to use this pump the ERC 10 must not be present in the instrument or timebase 3 Remove the coiled tubing and four straight pieces of tubing from the clip on the Dionex ICS 1100 or Dionex ICS 1600 rear panel see Figure 2 Lift up and remove the panel covering the RFIC ER compartment on the right side of the Dionex ICS 1100 or Dionex ICS 1600 see Figure 3 Tubing Chase Release tubing from clip Figure 2 RFIC ER Tubing and Tubing Clip Figure 3 RFIC ER Compartment Panel Doc 065295 03 10 12 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 5 Release the tubing from the clip on the left wall of the RFIC ER compartment see Figure 4 6 Locate the ER3a and ER3b column Tu
43. red for compatibility with RFIC ER operation 1 Turn on the pump and suppressor at the flow rate and current settings recommended for the installed separator column see Section 6 8 If the RFIC ER Wellness panel is not already displayed display it now For instructions see Section 8 1 Chromeleon 7 or Section 8 2 Chromeleon 6 8 To start the conditioning procedure click Condition Suppressor under Suppressor Information Chromeleon 7 or ERC 10 Controller Chromeleon 6 8 on the RFIC ER Wellness panel While conditioning is underway periodically check all tubing connections for leaks tighten any connections that leak To monitor the status of the conditioning procedure check the Remaining Time under Eluent Expiration Information Chromeleon 7 or Eluent Chromeleon 6 8 If the pump unexpectedly stops running and Chromeleon displays the Pump stopped due to RFIC message in the Audit Trail follow the appropriate steps to resume the conditioning procedure a Click Reset Gas Volume under ERC 10 Controller to enable the pump again b Click Reboot ERC 10 to prevent the ERC 10 from deactivating the pump for 3 hours Doc 065295 03 10 12 31 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 5 When the conditioning procedure finishes the system automatically shuts down and Chromeleon displays the following message in the Audit Trail The eluent has expired and needs to be replaced now The pump has been shu
44. s post reagent delivery and the CRD 300 e RFIC ER is not compatible with any eluents containing organic solvents The eluents in an RFIC ER system must be 100 aqueous e The capacities of the various trap columns in the RFIC ER system are limited therefore RFIC ER is compatible only with low contaminant water samples such as drinking water surface water and ground water 32 Doc 065295 03 10 12 RFIC ER System Operating Guidelines e During an injection on an RFIC ER system the equivalent volume of eluent is exchanged with the sample injected Because sample ions are trapped by the columns the eluent is diluted The RFIC ER system is designed to handle a pre defined volume of injected samples before the eluent needs replacement Injections of samples in excessive volumes whether due to a large number of small injections or a smaller number of injections with large sample loops will dilute the eluent and cause unacceptable retention time drift Chromeleon monitors the sample volume injected and displays a warning to replace the eluent when the limit is approached e When running sequences the RFIC ER Wellness feature uses the current injection volume to monitor the volume of sample that is injected into the system and to calculate the number of injections that remain Before the first injection Chromeleon may be unable to calculate the number of remaining injections however the affected eluent and trap column counters on the RF
45. t down to protect the guard and analytical columns 6 Dispose of the used eluent in the eluent bottle Prepare 4 liters of fresh eluent and add eluent up to the maximum fill mark on the bottle see Figure 9 7 Disconnect the ERC 10 outlet line from the eluent bottle and direct it to a waste container Operate the system for 30 minutes to flush out the expired eluent 8 After 30 minutes turn off the pump Reconnect the Eluent Bottle In tubing to the ERC 10 outlet 9 Add eluent up to the maximum fill mark on the eluent bottle see Figure 9 IMPORTANT If the eluent level falls below the minimum fill mark the outlet tubing may not reach the fluid level and the pump may lose prime The RFIC ER system is now ready for up to four weeks of nonstop operation Before starting operation review the operating guidelines in Section 10 10 RFIC ER System Operating Guidelines For complete operating instructions refer to the operator s manual for the Dionex ICS 1100 or Dionex ICS 1600 The guidelines here are intended to supplement not to replace the information in the operator s manuals e RFIC ER is compatible with carbonate bicarbonate eluents for anion separations and methanesulfonic acid eluents for cation separations only e RFIC ER can only regenerate the original eluent therefore RFIC ER is compatible with isocratic separations only and cannot be used with detection systems that alter the eluent composition such a
46. t of an action suggest how to optimize instrument performance etc ERC 10 Labels The TUV GS and cTUVus Mark safety labels and the CE Mark label on the ERC 10 indicate that the ERC 10 is in compliance with the following standards and directives EMC Susceptibility and Immunity e EN 61326 1997 A1 1998 A2 2001 e Low Voltage Equipment Directive 73 23 EEC e EMC Directive 89 336 EEC Doc 065295 03 10 12 3 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation Safety e EN 61010 1 2001 e CAN CSA C22 2 No 61010 1 2004 e UL 61010 1 2004 These symbols appear on the ERC 10 or on ERC 10 labels a Alternating current D Protective conductor terminal Power supply is on Indicates a potential hazard Refer to the user manual for an explanation of the hazard and how to proceed 2 3 ERC 10 Specifications Main Power 5 volts DC Typical input power 25 mW Typical line draw 5 mA Operating 4 to 40 C 40 to 104 F Temperature Operating 5 to 95 relative humidity noncondensing Humidity Dimensions Height 18 7 cm 7 375 in Width 5 7 cm 2 25 in Depth 3 4 cm 1 375 in Weight 0 38 kg 0 83 1b 4 Doc 065295 03 10 12 Functional Description Functional Description This section presents a brief overview of key RFIC ER components For more information about the trap columns refer to the column manual provided on the Thermo Scientific Reference Library DVD P N 053891 Figure 1 illustrates t
47. th the system not operating in the recycled mode of operation During this time periodically check all tubing connections for leaks Tighten any connections that leak After 1 5 hours check the eluent bottle If the eluent level is below the minimum fill mark see Figure 9 turn off the system refill the eluent bottle and restart the system If the eluent level falls below the minimum fill mark the outlet tubing IMPORTANT i may not reach the fluid level and the pump may lose prime 22 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems After 3 hours of total operation time shut down the system Remove the 1 8 in OD PTFE tube labeled To Eluent Bottle In from the waste container Allow the fluid in the tubing to drain into the waste container Disconnect the green tubing labeled SRS REGEN OUT from the suppressor SRS Regen Out port and allow the fluid to drain into a waste container Connect the end of the green tubing you just disconnected to a 1 4 28 union P N 039056 a luer adapter P N 024305 and a 10 cc syringe P N 079803 see Figure 18 Pull all remaining fluid about 16 cc from the ERC 10 until only air is exiting the controller 10 Figure 18 Drawing Fluid from the ERC 10 Remove the union luer adapter and syringe from the tubing labeled SRS REGEN OUT Reconnect the green tubing to the suppressor SRS Regen Out port Install the fitting and ferrule remove
48. tion Valve 18 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems 6 6 Installing the ER2 Column 1 Disconnect the black PEEK tubing that connects the Cell Out port of the detector cell to the suppressor Regen In port 2 Note the green tubing labeled Cell Out extending from the lower left tubing chase on the component panel Connect this tubing to the Cell Out port of the detector cell see Figure 15 Connect green Cell Out tubing to Cell Out port Figure 15 Connecting the ER2 Column to the Detector Cell 3 Inthe RFIC ER compartment remove the coupler connecting the two pieces of green tubing ER2 IN and ER2 OUT 4 Connect the tubing labeled ER2 IN to the inlet of the ER2 column 5 Connect the tubing labeled ER2 OUT to the outlet of the ER2 column Connect the other end of this tubing which is labeled SRS REGEN IN and extends from the lower left tubing chase to the suppressor Regen In port Doc 065295 03 10 12 19 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 6 Mount the ER2 column with the inlet end at the top onto the ER3b column using the remaining ER1 ER2 bracket clip P N 069671 from the RFIC ER Installation Kit Install the ER2 column to the left of the ER3a and ER3b column assembly see Figure 16 ER3b ER3a ER2 Column ER1 ER2 Bracket Clip Figure 16 Mounting the ER2 Column in the RFIC ER Compartment 20 Doc 065295 03
49. two ER1 ER2 bracket clips P N 069671 provided in the RFIC ER Installation Kit Install the ER1 column to the right of the ER3a and ER3b column assembly see Figure 12 ER1 Column Figure 12 Mounting the ER1 Column in the RFIC ER Compartment 16 Doc 065295 03 10 12 Initial Setup for Dionex ICS 1100 ICS 1600 Systems 6 Note the tubing labeled InjVIv P extending from the lower tubing chase on the component panel Direct the end of this tubing to a small waste container see Figure 13 Direct InjVlv P tubing from tubing chase to waste ONY Figure 13 Completing Connections for ER1 Column Conditioning 6 4 Conditioning the ER1 Column 1 Verify that the suppressor is off To avoid damaging the suppressor always turn off the suppressor before conditioning the ER1 column Although the pump flow is on during conditioning no flow will reach the suppressor 2 Set the pump flow rate to 2 0 mL min and turn on the pump 3 Rinse the ERI column with eluent for 30 minutes Make sure the suppressor is not activated during this time 4 After 30 minutes turn off the pump Doc 065295 03 10 12 17 Dionex ICS 1100 ICS 1600 RFIC ER Kit Installation 6 5 Completing the ER1 Column Connections Remove the tubing labeled InjVIv P from the waste container Connect the tubing to port P 2 on the injection valve see Figure 14 Figure 14 Connecting the ER1 Column to the Injec
50. xtended period of time 12 13 After 30 minutes of flushing eluent from the system turn off the pump Remove the ERC 10 outlet tubing from the waste container Connect the outlet of the ERC 10 to the Eluent Bottle In tubing 36 Doc 065295 03 10 12 RFIC ER System Maintenance 14 Add eluent up to the maximum fill mark on the eluent bottle see Figure 9 15 Restart the pump and suppressor The system is now ready for routine operation 11 2 Replacing the ER1 Column When to replace the ER1 column The RFIC ER Wellness feature in Chromeleon monitors the use of the ER1 column An Audit Trail message will inform you when it is time to replace the column The ER1 column cannot be regenerated Never operate an ER1 column beyond its expiration this may result in IMPORTANT A irreversible loss of capacity to the guard and analytical columns You can check the life expectancy of ER1 Expiration Information the ER1 column at any time by reviewing the status information on a the RFIC ER Wellness panel The Capacity Remaining 2 Ia example screen shot here is from Expiration Date 6 27 2012 Chromeleon 7 For details about these parameters see the following table Capacity Consumed Capacity Remaining Expiration Date Chromeleon 7 Reset ER1 Capacity Consumed 0 00 The percentage of the ER1 column that has been consumed When the Capacity Consumed is 100 the ER
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