ÄKTApurifier - GE Healthcare Life Sciences
Contents
1. 5 Installation of AKTApurifier 5 2 Installing the computer and the cables e Unpack and install the computer and printer according to the manufacturer s instructions Place them to the left of the system Do not switch them on CAUTION The mains power to AKTApurifier must be switched OFF before the UniNet 1 cabling is installed e Complete the UniNet 1 data communication chain by connecting a UniNet 1 cable between the computer and Pump P 900 CAUTION The UniNet connection to the computer MUST be made to the board with four LEDs 37 e If using a fraction collector connect the UniNet 1 cable 35 between Pump P 900 and the fraction collector and UniNet 1 cable 36 between the fraction collector and the computer e All other UniNet 1 cables are connected at delivery Fraction collector optional WARNING AKTApurifier must be connected to a grounded mains socket tt lli jill wilt I ein Fraction 0 i collector intl optional e Connect a mains cable 39 supplied between AKTApurifier and a properly grounded mains socket Do not switch on e If using a fraction collector connect a mains cable supplied 38 between the fraction collector and a mains socket at the rear of AKTApurifier e Complete the two first sections o2. finished The print out consists of a chromatogram and an evaluation of the test result e Ifthe gradient test result is OK the print out says Gradient linearity accepted e Ifthe step response test result is OK the print out says Step response accepted e Ifthe UV response test result is OK the print out says UV response accepted If any of the evaluated values falls outside the specified range go to section 6 5 Correcting faulty evaluation results AKTApurifier 14 Installation Guide 18 1140 44 Edition AA Installation test 6 Manual evaluation If your chromatography system differs from the standard configuration e g if optional components have been installed the automatic evaluation will not give a reliable result A manual evaluation is required 1 AKTApurifier Minimise the run data window to access the Main menu Click on in the results window and then double click on the Installation Test01 icon to open the result file Maximise the chromatogram window by clicking on DI in the upper right corner Click in the Curves window with the right mouse button and select Properties Click on the Curves tab and select the following curves to be displayed e Installation Test01 1 UV1 _265nm 01 SMTH e Installation Test01 1_UV2_254nm 02 SMTH e Installation Test01 1_UV3_280nm 03 SMTH e Installation Test01 1_Conc Press OK Double click on 4 in the upper left corner of the chromatogram w
3. AA i About this installation guide 1 1 About this installation guide AKTApurifier Installation Guide 18 1140 44 Edition AA AKTA purifier is assembled and fully tested before shipping For safe transportation however some components have been secured and thus need to be detached before the system can be tested and used Cables capillaries accessories etc are enclosed in paper boxes or in Box 900 located at the top of the instrument pile This guide describes how to install AKTApurifier 10 and ARTApurifier 100 The description is common for both systems unless otherwise is clearly stated in the contents The differences are few and indicated with divergent type face The guide is divided into two parts one describing the installation and one describing how to run the installation test After the installation procedure has been performed your AKTApurifier is ready for purification work For full details of specifications methods maintenance etc refer to the respective User Manuals and Instructions 2 Safety 2 Safety The system is designed for indoor use only Do not usein a dusty atmosphere or close to spraying water Refer to Technical Specifications in the System M anual for detailed environmental pre requisites WARNING The individual instruments must not be opened by the user T hey contain high voltage circuits that can give a lethal electric shock WARNING Monitor UPC 900 uses h
4. AKTA design AKT Apurifier Installation Guide Amersham e Biosciences Important user information A M eaning Consult the instruction manual to avoid personal injury or damage to the product or other equipment VVARNINGI The Warning sign is used to call attention to the necessity to follow an instruction in detail to avoid personal injury Be sure not to proceed until the instructions are clearly understood and all stated conditions are met CAUTION T he Caution sign is used to call attention to instructions or conditions that shall be followed to avoid damage to the product or other equipment Be sure not to proceed until the instructions are clearly understood and all stated conditions are met N ote TheN ote sign is used to indicate information important for trouble free or optimal use of the product Should you have any comments on this instruction we will be pleased to receive them at Amersham Biosciences SE 751 84 Uppsala Sweden Trademarks T A UNICORN and Superloop are trademarks of Amersham Biosciences or its subsidiaries Windows is a trademark of Microsoft Corp Terms and Conditions of Sale All goods and services are subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions of sale is available on request Addresses Amersham Biosciences SE 751 84 Uppsala Sweden Amersham
5. Biosciences UK Limited Amersham Place Little Chalfont Bucks HP7 9NA England Amersham Biosciences 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Postfach 5480 D 79021 Freiburg Germany Amersham Biosciences 2000 All rights reserved eC CONtents Contents 1 About this installation guide sssssssssessssrsresersrrrssr 1 2 Safety a ial keen a eee 2 3 Pre requisiteS urriela aan 3 4 Installation overview erre e ereeeeeizineenee 4 5 Installation of AKTApurifier ie 5 5 1 Unpacking sica nona ione gia sa ivi 5 5 2 Installing the computer and the cables 8 6 Installation test nr 6 1 Preparation of AKTApurifier 6 2 Priming and purging Pump P 900 n 10 6 3 Running the installation test method 12 6 4 Evaluating the installation test results 14 6 5 Correcting faulty evaluation results 17 MO SUPOCOR EE deine iaia latita 19 7 1 Gradient test result i 19 7 2 Step response test result ii 19 73 UN response test testilt virata 19 8 Installation record rrrrrererieeeeeezezinioneoo 20 9 Registration form eeseeeeesesssessesccoecssssssseseoscosesesesssses 21 AKTApurifier Installation Guide 18 1140 44 Edition
6. KTApurifier prepared Installation Test method run Installation Test results evaluated Test Record completed Registration Form completed Test Record and copy of Registration form stored in System Logbook Registration form posted to Service Administration Installation Guide stored in User Manual box for future use 20 AKTApurifier Installation Guide 18 1140 44 Edition AA Registration form 9 Xx 9 Registration form IMPORTANT WARRANTY REGISTRATION INFORMATION Please ensure that this form is completed and returned to Service Administration to register the users equipment under warranty N m rs ie nates Meeps eects ee o IS OD ela Institute company i aoe ae ASEE AEN aaraa ip ri Address anali Ra a ena ai i Deparunent lotanionis pia Gea ini Res ia cotta ve Dret ea isla Post COG 5555 055 ae a aa au nas acre da Phone Numberi ie Fax Nim befann a E e ei ER End USerS is ta ia ss oi ns E mallisiguaniania Golan Date of Installation ii QUOTE NO Sears het A a ass Customer Order No ii INvolceNozmiisti stria aan Support Agreement purchased with the instrument Y N TE YES sive detail sisi es tec mena AE a ls Installer name i Signature of Installer i Installation Accepted n Daten ses ia naar Note Fill in serial numbers over leaf AKTApurifier i Installation Guide 18 1140 44 Edition AA 21 9
7. LIST BEFORE METHOD START For column Any E 7 Click on Run 12 AKTApurifier Installation Guide 18 1140 44 Edition AA Installation test 6 8 When the Method window is shown click on Next and then Next again 9 Click on Start to start the installation test method Note Irrelevant information may be de selected by clicking in the Curves window with the right mouse button and then selecting Properties 10 Click on the Curves tab Y Axis Curve Style and Colour Flow Scheme Logbook Run Data Groups Run Data Colour Curves Axis r Display curves Select All Clear All Cancel Help 11 Select the following curves to be displayed e UV1_254nm e UV2_265nm e UV3_280nm Conc De select all other highlighted curves AKTApurifier Installation Guide 18 1140 44 Edition AA 13 6 Installation test 12 The curves may now be monitored on the screen as the test progresses The installation test method run time is approximately 30 minutes Installation Test01 1 Installation Test01 1_UV1_265nm Installation Test01 1_UV2_254nm Installation Test01 1_UV3_280nm El Gradient result ple Step response result 13 When the test is finished the printer automatically prints out the chromatogram and the test result 6 4 Evaluating the installation test results Automatic evaluation The system automatically prints out the test result when the test is
8. Registration form Components QTY Part Number Description Serial Number System rack Mixer M 925 Monitor UV 900 Pump P 900 pH C 900 INV 907 PV 908 SV 903 A SV 903 B Computer Computer display 22 AKTApurifier Installation Guide 18 1140 44 Edition AA
9. cm is required e AKTApurifier requires 100 120 220 240 VAC 50 60 Hz electrical supply with safety grounding e Cutting pliers are recommended for cutting plastic straps e A waste flask e The installation test requires the following solutions 1000 ml of distilled water for priming and purging the pump 500 ml of 0 4 acetone in distilled water 100 ml of 20 ethanol in distilled water AKTApurifier Installation Guide 18 1140 44 Edition AA 3 4 Installation overview 4 Installation overview Unpack KTApurifier en ad ee a ei tt 5 Detach secured items and install items enclosed 6 Unpack and install the computer iii 8 Connect mains power cabling iii 8 Connect UniNet 1 data communication chain cabling 8 Complete the first two sections of the installation record 20 Prepare AKTApurifier for the installation test 9 Run the installation test Method iii 12 Evaluate the gradient i 16 Evaluate the step respOnse scccsscesscessecesecesecesseceseeceeeeaeeeeneeens 16 Evaluate the UV response iii eine 16 Complete the test record iii 19 Complete the registration form iii 21 Complete the final section of the installation record 20 Store photocopies of all records and forms in the System Logbook Store the Installation Guide in
10. f the Installation record on page 20 e The installation phase of AKTApurifier is now completed To Fraction To mains socket collector AKTApurifier 8 Installation Guide 18 1140 44 Edition AA Installation test 6 6 Installation test The installation test checks the function of the solvent delivery and the UV monitoring system of AKTApurifier The installation test can also be used at any time to check the condition of the system e g after a prolonged stop Correct gradient formation is tested by producing a linear gradient and a series of concentration steps of acetone Correct UV monitoring is tested by monitoring the acetone concentration at 265 254 and 280 nm and calculating the absorbance ratios 265 254 nm and 265 280 nm 6 1 Preparation of AKTApurifier Start up of the AKTApurifier separation unit 1 Switch on the separation unit using the mains switch located to the left on the base platform Start up of the computer and UNICORN software 1 Switch on the computer the display and the printer according to the instructions in the manufacturer manuals 2 Log into Windows NT by first pressing Ctrl Alt Del and then clicking on OK gt 3 When the Windows NT desktop appears start UNICORN by i double clicking on the UNICORN icon 4 Select user default and enter default as password Click on OK AKTApurifier Installation Guide 18 1140 44 Edition AA 9 6 In
11. igh intensity ultra violet light Do not disconnect the optical unit while the lamp is ON WARNING KTA purifier must be connected to a grounded mains socket WARNING There must always be a sample loop or Superloop connected to ports 2 and 6 of the injection valve This prevents liquid spraying out of the ports when switching the valve which is especially dangerous if hazardous chemicals are being used WARNING Two people are required to lift the system WARNING Never block the ports on the outlet valve with stop plugs since this will create over pressure and may result in injury WARNING Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining and servicing the system WARNING If the system is turned around or the fraction collector removed the external capillaries and other tubings may become entangled in nearby objects and be pulled from their connections causing leakage WARNING Never place waste containers on the top of the system If they become full and overflow liquid may penetrate the system causing a short circuit AKT Apurifier 2 Installation Guide 18 1140 44 Edition AA Pre requisites 3 3 Pre requisites WARNING AKTApurifier must be connected to a grounded mains socket e Two people are recommended to lift AKTApurifier onto the working bench e To install AKTApurifier a working area of about 200 x 80
12. indow and read the absorbance for the steps corresponding to Installation Test01 1_ UV1_265nm 01 SMTH Move the vertical bar to the constant section of each plateau by dragging it Enter the absorbance values in mAU in column 2 in the Step response table of the Test record see page 19 leaving out the decimals Read the absorbance for the plateaus corresponding to 0 and 100 B for the curves click on the curve name to change the curve reading Installation Test01 1_UV1_265nm 01 SMTH Installation Test01 1_UV2_254nm 02 SMTH e Installation Test01 1_UV3_280nm 03 SMTH and enter the values in column 2 in the UV response table of the Test record see page 19 Click on Print under File to print out the chromatogram Installation Guide 18 1140 44 Edition AA 15 6 Installation test 16 Evaluating the gradient Place a ruler along the gradient part of curve Installation Test01 1_UV1_265nm 01 SMTH in the printed report The curve should be linear between 10 B and 90 B and void of discontinuities Evaluating the step response Calculate the relative adsorption plateau heights for curve Installation Test01 1_UV1_265nm 01 SMTH as follows e Subtract the base line value 0 B from each of the values in column 2 in the Step response table of the Test record see page 19 and enter the results in column 3 e Divide each value in column 3 by the base line corrected value corresponding to 100 B multiply by 100 and enter the resul
13. pe holding the capillary loops attached to INV 907 waste capillaries W1 and W2 and PV 908 waste capillary W3 Place the waste tubings W1 W2 and W3 in waste bottles and place the bottles in front of AKTApurifier or wherever convenient Unpack the inlet tubing fixed in a plastic bag taped to the buffer tray on top of Box 900 Make sure all items are securely fitted and that no capillary connection has worked loose or been tangled Attach the column holder enclosed in Box 900 above the UV cell Select a slot to suite the height of the column to be used AKTApurifier Installation Guide 18 1140 44 Edition AA AKTApurifier Installation Guide 18 1140 44 Edition AA Installation of AKTApurifier 5 Should the cover over the UV cell compartment come loose it is refitted in the following way e The cover is a simple push fit onto the cell holder Two small lugs on the cover locate in holes at the front and rear of the cell holder e The cover is then lowered over the cell holder The pH flow cell and pH electrode is not mounted from factory in AKTApurifier If you want to include these items follow the installation procedures described in the System Manual and the Monitor pH C 900 User Manual They are both included in the AKTApurifier manual box Note The installation of a fraction collector optional is described in the AKTAdesign Optional Configurations User Manual Refer to the section that describes your model
14. s 3 to 5 for the other pump heads AKTApurifier Installation Guide 18 1140 44 Edition AA Installation test 6 Testing pressure stability Perform a pressure test to establish that all air has disappeared from the pump heads Perform as follows 1 Connect a column bypass capillary between the injection valve port 1 and the top of the UV optical unit AKTApurifier 10 Run 0 2 ml min at 0 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 02 MPa AKTApurifier 100 Run 10 ml min at 0 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 2 MPa AKTApurifier 10 Run 0 2 ml min at 100 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 02 MPa AKTApurifier 100 Run 10 ml min at 100 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 2 MPa Proceed to step 5 if the pressure is stable If not consult the Pump P 900 User Manual for trouble shooting instructions Click on END Transfer the inlet tubing BI into a flask containing 500 ml of 0 4 acetone in distilled water Installation Test Method Guide Buffer A Distilled water Buffer B 0 4 acetone in distilled water Test flow rate 5 ml min in AKTApurifier 10 Test run time Approximately 30 minutes 10 ml min in AKTApurifier 100 AKTApurifier Ins
15. stallation test 5 Click on the System Control button in the task bar System Control button FE Start lt S UNICORN Main Menu lt 4yMethod Editor untitled 1 System Control 1 Bjorn y lt q Evaluation 6 2 Priming and purging Pump P 900 Immerse the rinsing tubing in a flask 1 containing 20 ethanol in distilled water Connect a syringe to the rinsing tubing that is connected to the underside 2 of the left pump head on pump A Slowly draw rinsing solution to the syringe When rinsing solution starts to enter the syringe continue to draw a few millilitres Loosen the syringe and immerse the tubing in the rinsing solution 1 Purge the Pump P 900 as follows 1 10 Immerse the inlet tubing of all pump modules with filters distilled water flask Note Never place the reservoir flask below the level of the pump inlet Connect a male Luer syringe of about 30 ml to the open end of the purge tubing Connect a male Luer connector at the other end of the purge tubing to the left purge valve at pump module A Turn the purge valve counter clockwise half a turn to open it and slowly draw eluent into the syringe When fluid starts to enter the syringe continue to draw a few millilitres before closing the purge valve Check that there is no visible air left in the inlet tubing Purge valve Outlet tubing Repeat step
16. stallation test This page is intentionally blank 18 AKTApurifier Installation Guide 18 1140 44 Edition AA Test record 7 7 Test record 7 1 Gradient test result Gradient linear from YB tO B 10 90 7 2 Step response test result Step response table 1 2 3 4 5 Programmed Value Baseline Normalised Allowed Conc B read corrected value interval value 100 95 94 96 70 69 71 30 29 31 5 4 6 0 7 3 UV response test result UV response table 1 2 3 5 Wavelength Value read Baseline Absorbance Allowed nm corrected ratio interval 100 B 0 B value 254 265 254 1 11 1 26 265 265 280 1 26 1 53 280 AKTApurifier Installation Guide 18 1140 44 Edition AA 19 8 Installation record 8 Installation record Check Sign Remarks 1 e e Unpacking Contents according to packing lists All packing material removed No visible damage Installation Injection valve waste tubings port 4 and 5 marked W1 and W2 to waste reservoir Outlet valve waste tubing port 1 marked W3 extended to waste reservoir Column holder installed pH electrode holder installed optional Computer and printer installed UniNet 1 cabling installed Mains power cabling installed Installation test Solutions prepared Tubings to piston seal rinsing system in 20 ethanol A
17. stem can be safely packed using the original packing material AKTApurifier Installation Guide 18 1140 44 Edition AA 5 5 Installation of AKTApurifier Turn AKTApurifier on its swivel platform to access the fluid component side of the system Detach the following items by cutting straps and removing red tape Also remove the desiccant bags Note Use cutting pliers Be careful not to cut any capillaries by accident Make sure not to lose any of the capillary marking tags e INV 907 located at the top right side Detach the valve from the attachment bracket to remove the protective foam and reposition the valve to the top slot in the attachment bracket e PV 908 located below INV 907 Cut also Cut here and remove foam Cut here the straps holding the On line filter and the Flow restrictor Detach the valve from the attachment bracket to remove the protective foam and reposition the valve to a slot one position up from its transportation position slot in the attachment bracket e M 925 located below PV 908 Detach the mixer from the attachment bracket to remove the protective foam and reposition the mixer to the same slot in the attachment bracket Remove plastic bag from inlet tubing e SV 903A and SV 903B located between the pump heads on P 900 UV cell cover located above the UV flow cell Remove the red tapes Capillary loops Remove the red ta
18. tallation Guide 18 1140 44 Edition AA 11 6 Installation test 6 3 Running the installation test method 1 Start UNICORN as described in the Making your first run booklet section 2 2 UNICORN Overview The Toolbar guide is displayed after start up Click on Close Select File Printer setup from the main menu bar Select the appropriate printer from the list and select Landscape Then click on OK to acknowledge the printer chosen Print Setup 21 x Printer Name 4 BIOTECHSRND1 TECH3 Properties Status Ready Type Tektronix Phaser 340 Where SPHARMACIA BIOTECHYAND1 TECH350C PS Q Comment Paper r Orientation Size AA hd A Portrait Source Paper gt Landscape Network Cancel Return to the Main menu screen re Click on the Instant Run button Select Installation Test in the templates list and make sure that Any is selected for both Technique and For column For system Purifier Ri r Template selection Technique Method notes Any TEMPLATE Installation Test version 2 00 Template Installation Test to check the function of the solvent delivery and the UV monitoring Chromatofocusing CIP system of ART Apurifier Follow the instructions in the Installation Guide to perform the run and to evaluate the result Manual Injection FP BufferPrep Manual Injection FP Isocratic Manual Injection FP Segmented Manual Injection FP Step CHECK
19. the User Manual box AKTApurifier Installation Guide 18 1140 44 Edition AA Installation of AKTApurifier 5 5 Installation of AKTApurifier Begin by creating a clean and dry working area of 200 x 80 cm that allows easy access Then follow the step by step instructions below and fill in the installation record as you go along see page 20 Note Some components are packed in Box 900 located at the top of the system Note Some packing lists are included in the paper boxes 5 1 Unpacking e After having removed the cardboard hood check the contents against the attached packing list Check also all included boxes Store all the enclosed paper boxes and plastic bags in a convenient nearby place e Release and remove the red strap 1 holding the system to the pallet e Lift AKTApurifier onto the work area using the four strap handles 2 Two people are required to lift the system Dont raise to upright position yet Lay the system on the same side as on the pallet e Release the two red straps with the strap handles Allow the straps to remain taped to the plastic cover e Pull back the plastic cover 3 to uncover the swivel platform 4 and raise the system to an upright position e Remove the plastic cover with the red straps from the system e Save all the original packing material If for any reason the equipment has to be repacked for transportation or otherwise it is important that the sy
20. ts in column 4 The values of column 4 should all fall within the intervals given in column 5 Evaluating the UV response Calculate the UV response ratios in the following way e Subtract the base line values 0 B corresponding to each UV curve from the values in column 2 of the UV response table of the Test record see page 19 and enter the results in column 3 e Calculate the absorbance ratios 265 nm 254 nm and 265 nm 280 nm using the values of column 3 and enter the results in column 4 The ratios obtained should all fall within the intervals given in column 5 AKTApurifier Installation Guide 18 1140 44 Edition AA Installation test 6 6 5 Correcting faulty evaluation results Should any of the evaluated values fall outside the specified range proceed as follows e Ifthe system differs from the standard configuration evaluate the result manually If the faulty evaluation result remains continue below Faulty gradient e The gradient is linear but the interval is too small the mixer chamber is too large or the mixer is faulty e Disturbances may arise from air in the pump pump valves or bad sealings in the pump Refer to the Pump P 900 User Manual Faulty step response e Tf all values are faulty air in the pump or a faulty pump e 5 and 95 faulty bad sealing in the pumps 5 faulty pump module B 95 faulty pump module A AKTApurifier Installation Guide 18 1140 44 Edition AA 17 6 In
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