EX2400 - Bionova
Contents
1. 2 1 2 To keep the instrument working placidly it is suggested to use the 220V 22V AC power source voltage stabilizer in where the voltage fluctuation fiercely 2 1 3 Keep the operating temperature at 10 40 C and the relative humidity under 85 2 2 Installation and usage Install the instrument and connect the power behind it 3 Instrument structure 1 Magnetic bar 2 Magnetic Cap 3 96 well plate 4 96 well plate transport shelf 4 Instrument operation 4 1 Function description of the instrument panel Oo EX2400 7288 El zh jE KX EX2400 Automated Nucleic Acid Extraction Shanghai ZJ Bio Tech Co Ltd Function key Start Stop Start Start to run the selected program Stop Press one time to pause the program push twice to stop the program Direction key Forward or backward circulation choose replaced program LCD Displaying the selected program name program running Real time action name and the run time 4 2 Operation procedure 4 2 1 Instrument start Connection power Make sure the power is off when connect the power cord with power socket Then turn it on Instrument self check when the instrument is turned on it will start a automatic self check before it is ready to use 4 2 2 Operation procedure 4 2 2 1 Sample pretreatment Different kinds of sample should be pretreated according to the user manual in the RNA isolation kits 4 2 2 2 1 Program the system according to the2. buffer 600ul sample 200ul magnetic beads 200ul B Washing Buffer A 600ul C Washing Buffer W 600ul D Washing Buffer W 600ul E Elution Buffer 100ul 2 Turn on the instrument Pull out the 96 well plate transport shelf after the magnetic bars and the caps move to the right place 3 Place the 96 well plate carefully on the shelf well A should on the left and put back the shelf 4 Insert the magnetic cap into the groove then push inside until hear click 5 Close the front cover to avoid the environmental contamination 6 Choose RNA Virus program according to the manual press Start to run the instrument 7 After the program finishing take out the 96 well plate and cap collect the eluted nucleic acid solution immediately then shift into the tube for the following experiment Otherwise the results should be stored at 4 C briefly or 20 C for a long time Distributore SP Via di Grotte Portella 6 8 00044 Frascati RM amp 7 Tel 0672632108 Fax 06 72676527
3. e Close down the plastic bag lay aside for at least 24 hours Take the instrument out and wash again with mild cleanser Note cleanser 10 formaldehyde or 75 ethanol or 4 glutaraldehyde 5 5 Guideline of the fault resolution When the instrument is turned on the system will start to self check When the check is ok the magnetic bars and caps should stay in the standby place and the LCD screen shows the current program If the check failed the instrument will auto alarm sound like didi Solution for the possible fault Fault Possible cause Solution The lead rail can The magnetic cap is not raised Press the Stop button and restart not move the machine The magnetic The place of the cap is not Check whether there is any block to cap place in the wrong place consistent with the signal of the inside sensor affect the up and down movement of the magnetic cap Check whether the cap is totally inserted into the groove The magnetic cap move overtime The cap does not get the sensor in the supposed time Check whether there is any block The lead rail in wrong place The lead rail is not consistent with the inside sensor signal Check whether there is any block The lead move overtime rail The lead rail does not get the sensor in the supposed time Check whether there is any block The magnetic bar in the wrong place The magnetic bar place is not consistent wi
4. performance parameter and specification Model EX 2400 Sample volume 20 200ul Sample guantity 24units time Sample handling time 20 40min Magnetic bead collection efficiency gt 99 96 well plate 2 Magnetic Bar 24 Cap single use 2 strips 12 holes strip Keypad Monitor Start Stop direction keys LCD Boundary dimension L W H 45 38 43cm Net weight 20KG Operation condition Room temperature 1 3 Operating principle Using magnetic bead isolation technique EX2400 system can extract the whole nucleic acid from the sample by the collect release divert of the magnetic bead See the main step in the process of nucleic acid extraction 1 Adsorption Add magnetic bead into sample lyses solution vibrate and blend adequately the released nucleic acid is adsorbed in the specific encrusting substance of the surface of the magnetic bead 2 Washing Collect and transfer the magnetic bead in the first procedure into washing buffer wash repeatedly to eliminate the impurity 3 Elution Transfer the magnetic bead elution buffer after vibrating and blending adequately target nucleic acid will drop from the surface of the magnetic bead and dissolve into the elution buffer 2 Instrument installation 2 1 Installation conditions 2 1 1 Place the instrument in the dry dustless vibration less place Avoid the moisture and direct sunshine Assure the instrument is placed on the stable worktable
5. Liferiver Automatic Nucleic Acid Extraction system EX2400 o OL Ox J ema info carlibiotec it QR Www carlibiotec it Contents Brief introduction of the system 1 1 Main application 1 2 Main performance parameter and specification 1 3 Operating principle Instrument installation 2 1 Installation conditions 2 2 Installation and usage Instrument structure Instrument operation 4 1 Function description of the instrument panel 4 2 Operation procedure 4 3 Application example Instrument maintenance fault resolution and considerations 5 1 Periodical preventative maintenance 5 2 How to clean well plate transport shelf 5 3 How to clean magnetic bar 5 4 Sterilization Procedure 5 5 Guideline of the fault resolution 5 6 Considerations Attachment A FAQ solution Attachment B The extraction sample 1 Brief introduction of the system 1 1 Main application EX2400 nucleic acid automatic extraction system adapts advanced magnetic bead isolation technigue Combined with magnetic bead isolation kit it can extract high purity nucleic acid from varieties of sample such as whole blood serum plasma feces milk and cells The instrument structure is well designed and easy to operate It is time saving labor economizing providing high efficiency and excellent consistency results EX2400 is specially applied for nucleic acid extraction in genome research molecular biology research and clinical genetic testing 1 2 Main
6. be replaced A1 The magnetic bars of EX2400 system is made by stable material The magnetic field will not weaken If something unexpected made it necessary to replace the bar we can also replace it For example if the bar is mechanically destroyed we can offer the replacing service by maintenance technicians O2 How strong is the magnetic field Will it affect those sensitive machines A2 The magnetic field is mainly around the tip of the magnetic bars It is limited and will not affect the circumambient machines O3 What if I forgot to put on the cap and cause the magnetic beads absorb on the surface of the bars A3 You can use the soft cloth or soft paper dipped with mild detergent soap or ethanol to wipe the magnetic bars Q4 What should I do if the magnetic beads are left in the sample wells A4 The following steps will not be affected if only a few beads remain in the wells If the sample is too sticky for the magnetic bars to absorb totally the beads you can first dilute the sample and check whether the sample can be homogenate or dissolved Attachment B the extraction sample Using Liferiver Automatic Nucleic Acid Extraction System EX2400 with RNA Isolation Kit to extract the Foot and Mouth Disease Virus FMDV RNA from the PBS dissolved stool sample 1 Add the reagents into the 96 well plate according to the RNA Isolation Kit manual Compared with the following table Well Reagents and volume A binding
7. eparation and purification kit to separate and purify the RNA Cell and tissue cultured in vitro and total RNA can all be used as raw materials Purified RNA can be used in downstream experiment for example RT RCR Operation steps see 4 2 2 Choose the RNA Virus program and then press Start to start the program Attention Specific operation step see the kit instruction 4 3 2 Whole blood genome DNA purification Whole blood genome DNA separation kits combination with EX2400 nucleic acid automatic extraction system can separate and purify the Whole genome DNA automatically Purified genome DNA can be used in a variety of molecular biology experiment including PCR and Restriction End nuclease Reaction Operation steps see 4 2 2 Choose the g DNA Blood program and then press Start to start the program Attention Specific operation step see the kit instruction 4 3 3 Virus DNA purification EX2400 nucleic acid automatic extraction system combination with the virus DNA purification kit can separate and purify the total DNA The sample can derive from the cultured and tissue Obtained high guality DNA can be applied in PCR reaction etc Attention See the detailed procedure in the kit manual 5 Instrument maintenance fault resolution and considerations 5 1 Periodical preventative maintenance Keep the instrument from dust and liquid pollution When finished the operation operator should turn on the inside UV light
8. th the signal of the inside magnetic bar sensor Check whether there is any block The magnetic bar move overtime The magnetic bar dose not get to the inside sensor in the supposed time Check whether there is any block kata noise The micro well plate is not fixed Refix the plate Make sure the plate is totally inserted into the groove The fixed The fixed parameters of the Ask the technician for help parameters instrument lost disappear 5 6 Notes Before using this instrument the operator should read carefully the manual and do exactly according to the procedure When shift the 96 well plate to the transfer shelf please be carefully and avoid the liquid spill out to pollute e Make sure the 96 well plate in the right direction A H on the plate should be on the outside of the shelf A on the left and H on the right Put the 96 well plate tight with the shelf without being crooked Otherwise the efficiency of the magnetic beads transfer will be limited When put on the magnetic cap put the cap inside until hear click to assure the cap is totally inserted into the groove Every time before putting on or taking off the 96 well plate the operator should be sure that the magnetic cap and bars are up raised and separated totally Attachment A FAO solution Q1 Will the magnetism of the bar die down If yes how long will the magnetism last Can the magnetic bar
9. to disinfect e Clean the instrument regularly to maintain a good exterior Use the mild cleanser like 75 ethanol to wash the screen keyboard and plastic cover Don t use the corrosive cleanser to destroy the glossiness of the paint e When the surface of the instrument is contaminated by bacteria we suggest to use dry cloth to wipe first and UV disinfect later If the contamination is bio hazardous materials operator should listerize it with temper sterilizing solution like Pasteurized liquid e No high pressure sterilization should be taken to either part of the instrument 5 2 How to clean well plate transport shelf e Try to keep the transfer shelf clean without being polluted by the dust or dirt At least once a week to clean the shelf using soft cloth dipped with cleanser or ethanol 5 3 How to clean magnetic bars Using soft cloth dipped with cleanser or ethanol to clean the bars 5 4 Sterilization Procedure If the sample is bio hazardous materials the following sterilization procedure or other related method is highly promoted e Prepare the sterilizing solution e Take out the 96 well plate and magnetic cap sterilized and abandoned Clean up the plate transfer shelf Wipe and listerize the surface of the instrument using 75 ethanol e Pack the instrument into a big plastic bag with the lid open e Put the soft cloth dipped with sterilizing solution in the bag Make sure the cloth dose not contact the instrument
10. user manual in the RNA isolation kits Place the pending sample washing buffer and elution buffer in the 96 well plate according to the designed order 2 Start the instrument raising the magnetic bar and cap pull out the 96 well plate transport shelf 3 Place the 96 well plate on the transport shelf carefully to the proper place well A should on the left 4 Insert the cap into the groove push slightly and Click sound shows the cap inserted completely 5 Close the front cover to avoid the environmental contamination 6 Choose proper program according to the manual press Start to run the instrument 7 After the program finishing take out the 96 well plate and cap collect the eluted nucleic acid solution immediately then shift into the tube for the following experiment Otherwise the results should be stored at 4 C briefly or 20 C for a long time 4 2 3 Power off Press the I O switch in the lower right corner of the instrument back close the nucleic acid automatic extraction instrument Dispose the 96 well plate and cap as biologic dangerous reject in time Use the mull or towel which dipped de ionized water detergent or suds to clean the transport shelf and the instrument surface if there has epidemical materials in the transport shelf sterilize with 75 alcohol or other disinfectant 4 3 Application example 4 3 1 RNA purification Use the EX2400 nucleic acid automatic extraction system and RNA s
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