Applied Biosystems 2720 Thermal Cycler
Contents
1. Go to Select User Name Stop or End of Run Stop pressed Confirm Stop Sample 50 1 Confirm Stop Press STOP to abort Press Resume to continue F1 F2 F3 Go to Run Time Stop pressed again End of Run 11 30 AM End of Run VAn Ne Edas Method General PCR Run completed at 11 30 05 AM 02 01 00 Length of run is 01 34 25 F1 F2 F3 F4 F5 History File History of method exp002 User adrian Reaction volume 50 pL Run started at 02 30 45 PM 02 01 00 Run aborted at 02 50 42 PM 02 01 00 Length of run 00 19 57 No exceptions Fl F2 F3 F4 F5 F4 FS At end of run Has method been stored Yes Go to Main Menu No v Method Not Stored Method Not Stored Press Store to save the method Press Exit to delete the method ore Go to Store Method on Instrument Notice to Purchaser Please refer to the Applied Biosystems 2720 Thermal Cycler User s Manual for limited license or disclaimer statements IMPORTANT Before operating this instrument be sure to read the safety information in the Safety and EMC Compliance Information section of the Applied Biosystems 2720 Thermal Cycler User Guide The user guide is provided in PDF on the CD that is shipped with the instrument If you are unable to access the CD you can view the user guide from the Applied Biosystems Web site http www appliedbiosystems com 2720 From this Web site you can also request fax or e mail deli2. adrian 02 10 00 exp002 adrian 02 02 00 Create Edit wv 1 Hild 3 Tmp 25 Cycles 2 Holds 94 0 94 0 72 0 You can highlight parameters and type in new values here The More key is present only when you highlight a temperature or time The Modify key is present only when you highlight a PCR temperature or time F1 F2 F3 The Insert and Delete keys are present only when you highlight any temperature Store Method on Instrument or time Store Method on Instrument Modify User april 1 Hldi 3 Tmp 25 Cycles 2 Holds Method exp002 n 94 0 72 0 Free Mem 122 methods 946 segments Accept User ethod Cancel F1 F2 F3 F4 F5 Go to Create Edit Go to Select User Name Deletes highlighted segment Method Name Y abcdefghi v Insert jklmnopgrj 2 Pre PCR 3 Tmp 25 Cycles 2 Holds Method Name xp00l stuvwxyz 94 0 94 0 i 17t t Use ENTER key to select a character N F1 F2 F3 F4 FS y Select Modification at Select Modification AutoX Increments or decrements time or temperature at the completion of each cycle 72 0 72 0 Inserts a hold or cycle to the left of the highlighted time or temperature Define Programmed Pause Start first pause at cycle of 25 Pause every 25 cycles for 00 30 Cancel 2 Holds Inserts a pause Then you can edit the pause or go to Create Edit User Main Menu 08 00 AM 09 25 00 Applied Biosystem
3. Applied Biosystems 2720 Thermal Cycler Quick Reference Card Run IMPORTANT Before operating this instrument be sure to read safety information as instructed on the back of this card Main Menu 08 00 AM 09 25 00 25 090 Applied Biosystems Thermal Cycler Version User adrian Run Create Edit Util F1 F2 F3 F4 F5 _ Stored Methods Methods User Size LastUsed applol adrian di 03 04 00 exp000 adrian 10 02 22 00 exp001 adrian 12 02 10 00 exp002 adrian 13 02 02 00 Go to Select User Name v Select Method Options Select Method Options Enter reaction Reaction Volume 50 pL volume here View Method 2 Hild 3 Tmp 25 Cycles 2 Holds 94 0 94 0 Enter a value from 5 to 100 pu Start 72 01 72 0 0 30 5 00 F4 F5 FL F2 F3 n j EA Method exp 001 Run Time Sample 56 7 Cycle 1 of 25 94 0 94 0 72 0 72 0 0 30 5 00 Sorting Criteria Sort Methods By Method name Date last used Date stored Method size F1 F2 F3 F4 F5 Method Information Sample 56 7 Cycle 1 of 25 02 32 PM Information 552 C i User lisa Method General PCR 72 0 72 0 Run started at 01 32 30 PM 03 01 00 Run will end at 06 35 30 PM 03 01 00 Reaction vol 50 pL Create Edit Main Menu 08 00 AM 09 25 00 Applied Biosystems Thermal Cycler Version Stored Methods User adrian Methods i LastUsed applol i 03 04 00 exp000 02 22 00 pL B2 F3 F4 F5 exp001
4. s Thermal Cycler Version User adrian Select User Name Select User Name ab dave jenife murray adrian doug mamood nayana anna faye mike ngui T F2 F3 F4 F5 Go to Main Menu It s gone unless you have a method User Name abcdefghi Highlight each letter jklmnoparj then press Enter User Name ane S Do you have a PIN number to spell the name Use ENTER key to select a character cel No Security Code 4 ee Security Check Protection Unlocked Security Check Press PIN to create a Then you set protection to Locked to prevent methods To perform this action you must enter from being overwritten or deleted your PIN number Cr cancel Your PIN F1 F2 F3 F4 F5 Accept Cancel Go to Select User Name New PIN Number Create a PIN Number Your PIN number protects the access to your user name and protection level Go to User Name Enter a PIN number New PIN XKXK F1 F2 F3 F4 ES PIN Confirmation w Protection Status V Username hank PIN number XXXX Protection Unlocked Confirm PIN Number Your PIN number protects the access to your user name and protection level Enter a PIN number again PIN XXXX Press PIN to create a Then you set protection to Locked to prevent methods from being overwritten or deleted F1 F2 F3 F4 F5 Press Accept to confirm your PIN Cancel Toggles Locked Unlocked
5. very In the United States and Canada you may call 1 800 762 4001 In other countries contact your local sales office P N 4359451 Rev B
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