LabSmith SVM340 Synchronized Video Microscope User Manual
Contents
1. Video Standard NTSC_M 4 2 VIDEO AND ILLUMINATION TIMING The master clock for the SVM340 is provided by the video signal timing The CCD camera outputs video in standard RS170 format NTSC in the color version which is an analog interlaced format compatible with standard analog video monitors or video recorders 28 Frame 33 ms full frame Fields Even Odd Even Odd ee AN SN Sf NULL mM LED drive Mice act Wee AG Meee LED on variable Timing sequence of the illumination in relation to the video signal The RS170 interlaced video signal is composed of two fields called even and odd fields each containing every second line of the image The interlaced format was defined in the early days of television to avoid flickering TV images The even field contains lines 0 2 4 524 and the odd field lines 1 2 325 The field frequency is 60 Hz with one even and one odd field adding up to a full video frame each 33 3 ms corresponding to 30 Hz frame frequency To ensure that all lines of the video signal are equally illuminated the LED s flash twice during an image once in every field The LED pulse starts in the frame blanking period and its width can be varied from 0 to near 100 of a field period 16 6 ms Due to the interlaced readout of the camera sensor images of fast moving objects which move a noticeable distance during the 16 6 ms between two con2. the outer mounting holes are used for the iBB g g Integrating Delrin stage shown assembled with LS600 CH left and iB right breadboards 19 3 USCOPE SOFTWARE The uScope software lets you set the functions of the SV340 and control the video acquisition and on line processing It also allows you to recall and process stored video files uScope runs on any PC with Microsoft Windows XP operating system or later Note The uScope application makes extensive use of the DirectX software which is provided by Microsoft Corp and installed independently of uScope If you have installed a local language version of Windows XP DirectX will install in the same language Consequently some of the dialog boxes shown below may appear in the language of your windows installation When the uScope software is started a dialog box will pop up that says Select a new serial port Select the COM port that the serial connector is connected to and click OK This dialog will keep popping up until you succeed in communicating with the SVM or you click Cancel then you work offline and uScope does not try to send commands to the SVM gt Select a new serial port AS Select the serial port for communicating with the SVM340 Serial port sq Open Windows R device manager ae Gem Communications settings dialog box Note Some
3. DOS ah WASEMA kin NG Ree d h i Black and white level adjust example a No adjustment b black 50 white 200 c black 100 white 150 d black 150 white 150 Flash Settings Do not strobe Illuminator will not change when snapshot is taken Strobe illuminator Illuminator settings will change when snapshot is taken Selecting LED Settings button opens the LED Pulse Durations dialog box where the snap shot illuminator settings can be selected After the snap shot is complete the illuminator will switch back to the original settings LED pulse duration dialog box 38 This function is useful to prevent photobleaching or heating of your sample With this option the illuminator is typically turned off or very low prior the snap shot Post strobe delay before recording Choose the number of frames to skip after the snap shot button is selected and before it takes the photo File naming This option allows you to set the default for the type of file compressed jpg or uncompressed bmp and the naming The files can be autonamed or you can choose to be prompted each time to choose the file name This window can also be access from File gt Snap Shot File Saving Type of file Compressed bitmap images jpg Do not auto name prompt for name Auto name files File path and name root C Users Eric Cummings4Documents4u5 cop Append the date YY MM DD Append the time HH MM 55 V A
4. LEDs each and two channels C and D have 4 LEDs each Available LED colors include Color Wavelength range Blue 464 476 nm Green 520 535 nm Red 615 636 nm Yellow 585 595 nm White Standard illuminator modules Name Channel A ChannelB Channel C Channel D LED B Blue Blue White Blue LED G Green Green White Green LED R Red Red White Red LED Y Yellow Yellow White Yellow LED W White White White White LED X Blue Green White Red LabSmith can provide customized illuminator modules with any combination of LED channels The illuminator module can be removed from the traverse from the top by pulling the module upwards Illumination module installed in SVM340 Note When reinserting the illuminator module take care that the connector pins are all correctly inserted in the receptacle without bending or damaging the pins Also take care not to apply excessive force Support the camera module from below with your hand when inserting the illumination module and press from below to ensure the traverse mechanism is magnetically seated 2 2 Optics module The optics module is attached to the traverse by magnetic holders and can be removed by tilting the SVM340 on its side and gently pulling the module down from below the SVM340 body until it comes free Important Turn off power to the SVM340 before removing or inserting an optics module 10 Optic
5. for the calculations Show Text turns on and off the text based velocity display Show Vector displays an arrow in the direction of the flow The size of the arrow will change with velocity You can also set the Scale to increase or decrease the arrow size Polygon and Macropixel intensity probes Intensity or polygon probes are used to track the color spectrum or fluorescence intensity inside a defined region The advantages of the polygon probe are that 1 you can make the probe an arbitrary shape to fit your region of interest and 2 for color images the probe can independently track the intensity of the red blue and green signals The macropixel probe is limited to a rectangular shape however it can be broken into multi pixel arrays to obtain spatially resolved intensity data The macropixel probe also allows time averaging of probe output data 49 6 2 1 Polygon intensity probes To create a probe 50 1 Click the Intensity Probe toolbar button io to highlight the button 2 With your mouse curser over the image right click on the mouse and select New Probe Click on the image to draw the outline of the probe When the shape is defined double click the mouse to close the polygon A probe such as the one below will appear NG Pay ae a ott yi bas WENG MANGA a lds Tatehisity ee 3 Repeat step 2 to create as many probes as desired Once created the probes can be moved by clicking and holding the mouse bu
6. functions are available with the SVM in offline mode Manipulation of the stage x y and z and illuminator settings can be accomplished via the front panel Images can be viewed and recorded 20 with the SVM operating offline however real time measurements PIV and Intensity probes are disabled The uScope main window shows the video display live or saved as selected in the Video menu The following controls are also available e probes e illumination e focus e x y traverse e presets e video recording File Edit View SVM Video Help acu Wuminator Focus Presets Gang x Ajj Bij kc Dj LE Ogam 7 8 Save O C F G 1 H J D Je Record from 099 4 ago now For durakon 1s 1000 Gals m 03090 O Unlimaed Advenced Ready 30 24 fps NUM uScope main window 21 X Y Traverse The position indicators to the right and below the video display indicate the position of the x y traverse and can be used to move the traverse The X Y traverse has a motion range of 50 mm by 75 mm The traverse can also be adjusted using the keyboard arrow keys Holding down the shift key while using the arrow keys slows the traverse to allow fine adjustment Focus The focus indicator slider bar is located at the top of the main window The slider bar can be used to adjust the focus while the and buttons to the left of the bar are used for fine adjustment The focus can also be adjusted using the Page Up and Pa
7. line uScope can open a stored video file for playback and further processing uScope will go into off line mode whenever it fails to locate a SVM340 on the selected serial port 3 3 Upgrading firmware The firmware is the software stored inside the SVM340 in non volatile memory and controls the internal functions of the instrument such as traverse movements front panel lamps and buttons and back panels inputs and 24 outputs The firmware is included in the uScope application package and can be loaded into the SVM340 from within the uScope software To upgrade firmware to the latest version 1 Download the newest version of uScope from www labsmith com and install on your computer following the installation procedure described in the introduction 2 Connect and turn on the SVM340 and start up the uScope application on the PC 3 In online mode choose SVM gt Update Firmware gt Update All SVM Video Help Magnification Set Serial Port Digital Outputs amp Inputs LED Settings Mation Limits Update Firmware Update All Versions Update Main Update Front Panel 4 Click OK when the update dialog box appears 5 Wait while the firmware is updated You can follow the progress in the status bar at the bottom of the uScope main window 6 When the progress indicator reaches 100 the upgrade is completed Important Do not turn off or disconnect the SVM340 or the PC while the upgrade is in progress This ma
8. store a traverse position press one of the storage buttons and hold it down a few seconds until the button light goes off This will store the current traverse position and the LED settings in the selected storage cell Note There are a total of 10 preset positions available on via the uScope software In LIGHT mode the buttons A D represent four LED channels labeled A D The 24 LEDs in the illuminator module are divided into four channels which can be controlled individually When a LED bank is selected the corresponding button lights up One or more LED banks can be selected simultaneously by pressing the one or more of the A D buttons Note When an EPI Optics Module is installed in the SVM the EPI illumination is controlled using front panel button D In this case illuminator channels B and D are both controlled by front panel button B In SITE mode the four buttons will move the traverse in the x and y directions Pressing a button will start the traverse motor at low speed and after about two seconds motor speed slowly ramp up to high speed Pressing the button briefly allows single stepping of the traverse Pressing the center button Stop will immediately stop any traverse movement which may be in progress When in LIGHT mode the up down keys will increase decrease the intensity of those LED banks which are selected by the A D buttons One or more of the LED banks can be controlled simultaneously Pressing the c
9. sure you have the latest drivers If you have purchased a RS232 USB adapter you will also need to install the drivers for this device Again the disk with drivers and instruction manual will be included with the product Your computer may need to be restarted after the video capture or RS232 adapter drivers have been installed 1 5 2 Installing uScope If your computer does not already have DirectX9 0 or greater installed you will need to install it before running uScope DirectX9 0 is free and available directly from Microsoft s website Most computers will already have this software installed To install uScope run setup exe and follow the on screen directions 2 SVM340 HARDWARE The following section describes how to set up your SVM and how to use it independent of the uScope software The SVM340 is shipped with the feet removed to protect the unit during shipping They must be installed on the unit before an optics module is inserted Note The SVM uses a sheet metal chassis to minimize weight size and cost The sheet metal chassis occasionally can have a slight warp that causes it to rock when the feet are attached The feet are designed to have some adjustability to accommodate for this unevenness This warp does not affect the function of the SVM due to the kinematic design of the focus stage 2 1 Illuminator module The illuminator module consists of four independent LED channels Two of the channels A and B have 8
10. then right click and select Properties The following dialog box will appear End to end distance 252 41577 Angle 30 degrees Display options Color Red Green Blue 255 255 0 Line thickness 2 Y Show end bars v Show distance Show angle Cancel Triggering Distance angle Indicator Properties dialog box 44 6 PROBES uScope has sophisticated real time probe capabilities that allow users to monitor image properties like color intensity variation and video properties like inferred motion e g particle image velocimetry in real time These real time measurements can be recorded to disk and can trigger real time actions uScope can support as many probes as your computer s processor can handle The two types of probes that can be made in uScope are the Velocity PIV probes and Intensity probes 6 1 Velocity PIV probes uScope software makes it easy to create probes to monitor flow characteristics Each probe can have its own properties probes are almost always square though it is possible to extend the probe in one direction to increase signal to noise ratio along that axis TIP When learning how to use uScope s PIV probes it is useful to start with a stable particle flow or a movie of a stable flow 6 1 1 Setup 1 Connect the SVM to the computer and turn on both 2 Launch uScope software 3 Prepare the microfluidic channel Fill it out with the buffer and int
11. x 75 mm opening external video capture device power RS232 and S video cables uScope software installation disk user s manual The following optional items may also be included if ordered additional optics module i e Color EPI additional illuminators 4X or 20X objective integrated breadboard sample stage e chip viewing sample stage two openings to fit most standard microfluidic chip sizes e internal video capture card e Schott glass filter with foam washer e Schott glass filter kit includes 8 filters and 2 foam washers If any parts are missing or damaged please contact your local dealer or LabSmith immediately 1 3 Basic functions The SVM340 combines an inverted fluorescence video microscope with a programmable synchronizer and software for on line image acquisition processing and storage You can use the instrument in several ways e Asa stand alone video microscope Connect the video output from the SVM340 to an analog video monitor or VCR through the BNC or the S video outputs on the rear panel You can now focus traverse and adjust illumination intensity by the controls on the front panel while observing the image on the monitor e Asa software controlled video microscope for automatic or manual acquisition of video sequences using the on line image processing and storage capabilities of the uScope application e Asan integrated part of a complex experiment synchronizing pulsed illumination image acqui
12. We Labsmith TOOLS FOR SCIENCE SVM340 Synchronized Video Microscope User s Guide uScope software 2009 2013 LabSmith This manual 2013 LabSmith No part of this document may be reproduced without the written consent of LabSmith Table of Contents TS INTRODUCHO Ni 3 1 1 Newin uScope Version 1 04 sseceeeeeeeeeeeeeeeeeees 3 1 2 Package contents Rumah 5 1 3 Basic functions anakan nma 6 1 4 Computer requirements ccccccccssesssseeeeeeeeeeeeeeeeeeeeeeee 6 1 5 Installing the software La GNG NANANA 8 2 SVM340 HARDWARE s sssnnssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 8 2 1 Illuminator module lt lt lt lt sssmmmmwmmznzunummwwaazzzzzzz 9 2 2 Optics MOANA usia 10 2 3 Microscope objective cccccccessseseeeeeeeeeeeesseeeeeeeeees 11 2 4 Fluorescence filter sseseeesssssssesesesessseseneneeees 12 2 9 Base Sand asana wawa wawa wawa wanawa wa sawasawa sawasawa sawasawa wawa wawaKaa 13 2 6 Front panel controls mmmmmmm mimi nini mimi nzi zzzi 13 2 7 Back panel connections seeeeeeeeeeeeeeeeeeeeeees 15 2 8 Microscope StaGe ssssseeeeeeeeeeeeeeeneeeeeceeeeeeeeeseneeeeeeeees 17 3 USCOPESOFTWARE 2 aaa 20 3 1 O Cg AA 24 3 2 Online and offline operation s eeeeeeeeeeeeeeeeeeees 24 3 3 Upgrading firmware 111 2mmmmmasssaaaaaaaaaaaaasasasaaasasaaaan 24 4 USCOPE VIDEO PLAYBACK AND PROCESSING 26 4 1 Video
13. adjust adjust typ high typ high typ high typ high Hue no effect no effect adjust no effect no effect adjust Saturation min min max min min max Sharpness min min adjust min min adjust Illuminator Settings White LEDs off off adjust adjust adjust adjust typically channel C Colored LEDs max off adjust adjust adjust adjust EPI LED channel n a max n a n a n a n a D EPI External light off off off off on on For fast moving flows we recommend using deinterlacing to eliminate lines across screen For steady images use time averaging to improve signal to noise 60 7 4 Dropped frames The SVM uScope software uses a buffer as it is recording to minimize dropped frames However if the available RAM isn t sufficient you may still experience dropped frames Improving available RAM e Set the RAM buffer should be gt 500 MB View gt Video Buffer Settings e Close anything running in the background that might be a resource hog i e Windows Indexer Antivirus scan fetching and scanning emails automated backup etc e If you are using uScope Probes close any that aren t necessary e Try running without interlacing and or compression e Color Format Running in true color Video gt Color Format gt 24 bit RGB uses 3X more RAM compared to the B amp W video options If true color is not necessary chose another option 61 SPECIFICATIONS Traverse Range Resolution Sample stage Dimensi
14. anced Settings for Time Lapse Note the settings selected in this window will only be applied to the video recordings the snap shot settings will not change The time between images in a time lapse video is dependent on the averaging time the wait time between recorded frames For example for a recording rate of 30 fps if you set the averaging to 15 frames and then set the wait time to 1 second then the apparent increase in speed for the video is 45X 15 frames averaged 30 frames skipped For live video recording advanced time lapse settings also allow you to control the illuminator strobe to synchronize with the time lapse rate 5 9 Distance indicator The Distance Indicator feature allows you to measure features on your image To create an indicator 1 Click the Distance Indicator toolbar button r3 button 2 With your mouse curser over the image right click on the mouse and select New Probe Click on the image to insert the indicator The ends of the indicator can then be moved click and drag to the desired locations to highlight the 43 Note The distance calculation is based on the magnification setting for the objective If the magnification setting is incorrect the distance will be incorrect If you are evaluating a saved image you will need to know what magnification was used and adjust the magnification at SVM gt Magnification gt Distance Indicator display data can be selected by selecting the probe
15. anges the number of pixels per sub probe The probe data can also be time averaged to reduce noise The following image shows two probes of different pixel size Edit View SVM Video Help ERB Hamna Geng eBam 8 D Example of macropixel probes Probe on left had array of 8 x 8 pixel size probe on right has 32 x 32 pixel size 52 6 3 Recording probe data Data can be recorded simultaneously from all probes To record data 1 Choose File gt Measurement File Naming to select how the recorded data will be saved Type of file Tab delineated text spreadsheet files dat v Do not auto name prompt for name Auto name files File path and name root C Users Eric Cummings Documents uS cop Append the date YY MM DD Append the time HH MM 55 V Append counter starting at 1 Example C Users Eric Cummings Documents uScope SVMO0001_1 dat Naming settings for new measurements dialog box If you choose Do not auto name uScope will prompt you for a file name and location for each new recording b Choose Auto name files to automatically name each recording Check Append the date Append the time and or Append counter to add these values to the new file names An example of how the name will appear is shown at the bottom of the dialog box a You can currently only save the probes in the tab delineated text spreadsheet file format Note 53 2 To begin recording
16. can change each probe s properties individually 6 1 3 Velocity Probe Properties 1 Double click on a probe to open the PIV Probe Properties dialog box You can also right click on the probe and choose Properties to open the dialog box 46 Subimage size Width Height 128 x x 128 pixels Averaging percent per frame 0 none Background mean rms 39 Correlations auto cross 95 Calculation options Time interval 12 frames 1 use consecutive images IV Subtract mean F Deconvolve autocorrelation Display options WI Show field Cross correlation V Show text V Show vector Scale 5 PIV Probe Properties dialog box 2 Select the Width and Height of the probe window the area over which statistics will be calculated Some guidelines for setting the probe window size are a Probes should typically be the same size in both x and y directions b Smaller probes require less processing power so use smaller window sizes to run more probes simultaneously c Increasing the probe size will improve the signal to noise ratio decreasing the size will increase spatial resolution d If the probe is located in a region of fast flow the probe size must be large enough that the correlation does not fall beyond the window The Cross Correlation field see below can be an aid in setting the size Note If you cannot achieve sufficient signal to noise ratio with a required probe siz
17. capture device is not listed in the Detected Video Capture Hardware Window ensure that the device drivers are correctly installed and the capture device is plugged into a USB port on the computer Make sure that either the BNC composite or S Video connector output of the SVM340 is connected to an input of the Hauppauge card and select the relevant input connector through the Input connector box Click Video gt Physical Input Connector and select the correct input and ouput if using an S Video cable select SVideo In typical If using the BNC connector select Video Composite 27 Properties AA an Crossbar Input Output 2 Video SVideo In EJ 0 Video Decoder Out v 1 Video Composite In 2 Video SVideo In 2 Video SVideo In Related Pin Related Pin 4 Audio Line In 1 Audio Decoder Out Link Related Streams Physical Input Connector selection box You do not have to worry about the audio options since they are not currently used for the uScope application At this point you should see live video on your screen To set the video frame size you can click Video gt Video options gt Video frame format Select 320x240 for low resolution images and 720x480 for full resolution images Do not change the color space whatever comes up normally RGB24 is correct Note The DirectX video controls dialog boxes are of general nature and allow settings incompatible with the SVM340 Do not change the
18. choose File gt Record or click the Start Stop a toolbar button If Autonaming is selected recording will begin immediately Otherwise recording will begin after you name the file and click OK 3 To end recording choose File gt Record or click the Start Stop button again Probe output file Data from all recorded probes will be output into a single file PIV probe data The PIV data will include four columns for each probe the X and Y locations of its centroid measured from the upper left of the window and the X and Y velocity at each point in time The X Y location columns will only have entries in the first row Note The probe data will only be recorded while the video is playing Snap shot images or paused video will not record probe data Polygon probe data The Polygon probe data output depends on the options selected in the Polygon Probe Properties window The first two columns are the horizontal and vertical pixel location of the centeroid referenced from the top left corner of the image The data columns are listed next and depend on the display options selected in the Polygon Probe Properties window Macropixel probe data The macropixel probe data contains four columns for each probe Macropixel sum from X Y defines the location of the macropixel probe X and Y are the horizontal and vertical locations respectively of the top left corner of the probe Span x y n defines number of pixels in the horizon
19. dule when a filter is installed 57 Very dim imaging select video settings mis Video gt Brightness Contrast Hue etc gt VideoProc image adjusted Amp and adjust the Brightness and Contrast settings incorrect e for fluorsecent imaging with B amp W optics excitation or module ensure the illuminator wavelength is filter the correct excitation for the fluorescent dye being used Also ensure that if a filter is installed under the objective its wavelength range is correct for the fluorescent emission e for fluorsencent imaging with the EPI optics module ensure the EPI optics module has the correct excitation emission for the fluorescent dye being used SVM can t targetabove The top focus range of the SVM is limited to focus on maximum prevent interference between the illuminator target focus range module and the stainless steel stage If the target can t be brought within the focus range one option is to use an objective spacer to increase the focus height by 1 25mm This solution works best when using an objective with a long working distance i e 4X and without the 4 channel illuminator i e with the EPI Optics Module no EPI incorrect Select View gt Camera Configuration and ensure illumination optics Epifluorescence Illumination Type is selected module NOTE ensure you have an EPI optics module configuration installed before you change the configuration EPI EPI illuminator adjustme
20. e you may need to adjust the illumination particle feed etc 47 48 If the probe is in an area of steady flow then increasing time averaging may also help 3 The Averaging percent per frame values help separate the useful flow information from the background data by weighing the previous frames of data versus the current frame The default values of 95 are acceptable for most flows Some guidelines for setting the percentages are a The Background mean percentage determines how much of the image field is considered background based on its steady presence over multiple frames Increasing this value increases the amount of information that is ignored such as stuck particles Check the Subtract Mean box see below to apply this calculation and remove the data Decreasing the Correlation value improves time resolution increasing the value improves noise control 4 The Calculations Options control how the flow parameters are calculated a Note Enter 1 in the Time interval box to calculate cross correlation for every sequential pair of frames Enter 2 to use every other frame 3 for every third frame etc This option is useful for examining very slow flows Subtract Mean subtracts the background non moving data from the flow calculations The amount of data that will be subtracted is based on the Background value see above If the probe is located in an area of very slow flow then subtracting the back
21. ene motion is continuous between frames When the movies are analyzed by various algorithms the effect of the deinterlace filter on the result will be algorithm dependent 5 8 Time lapse The Time lapse option is used to select the frame rate frequency to allow videos taken over a long period of time to be compressed to a small file and played back at increased speed The time lapse options are set by clicking on the Time lapse button Time lapse at the bottom left side of the main window The Time Lapse Settings dialog box will appear i Apply avanced settings Ri Strobe averaging etc Awa need cottons a Wait extra time between 1000 recorded frames Output format Custom playback i 1000 frame time AVI video JPGs sequentially numbered image files BMPs sequential uncompressed image files Output file naming Time Lapse Settings dialog box Clicking on the Advanced Settings button will bring up a window that is identical to the Snap Shot Settings window 42 Snap Shot Settings ii Flash Settings applied only to live video Black level adjust 9 Do not strobe None Strobe illuminator LED Settings 9 Auto set image min to black Poststrobe delay 495 me Manual 0 of 255 before recording Averaging White level adjust 9 Do not average EGE O Auto set image max to white Manual 255 of 255 Average 10 frames Averagefor 1000 ms Gene Adv
22. enter button Stop with switch off all selected LED banks Focus These buttons will move the focus motor up or down to focus the up down image The focus motor will start at slow speed and then ramp up to high speed Lock The lock indicator LED on the front panel is green when the indicator SVM340 is in position The Lock indicator LED turns red when the SVM340 is in motion e g while the traverse is moving to a preset 2 7 Back panel connections Video Output Digital Inputs and Outputs Ca Se p Power GOOOOOUE Video outputs SA OO hA InB Int nD Out 1 Out 2 Trigger Out mila aS hie SEE Ext Illuminator RS 232 Ma VEE DARK panel External Illuminator Communication Romer Plug the power cable from the back of the SVM340 into a 90 240 VAC power outlet Communication The female 9 pin D sub connector is for RS232 communications This link allows the SVM340 to receive programming and commands from an external controller e g a computer running the uScope application or LabView 15 Video Output Digital Inputs and Outputs 16 through the provided serial cable A USB RS232 converter can be used for computers without an RS232 port The video signal is typically output via the S video connector An analog video capture card included with the SVM340 is used to connect the S video to your computer It is generally recommended to use the S video for best image quality A BNC RS 170 output is also provided for use with monit
23. es pre trigger recording enabling you to store a video of what happened before the trigger instant 5 1 Video compression uScope can use video compressors that are installed on your computer but does not have any built in compression To add compression functionality you need to download and install a codec A compatible codec will also need to be installed on anything that plays the video All codecs are not equal Some compress well Some compress fast Avoid over compressing the data The compression occurs when the video is being captured so it cannot be undone if the results are not desirable If you plan to perform PIV on your data we recommend using a lossless compression The best quality video compression takes time It is often best to save videos with minimal or lossless compression and later post process them The uScope software contains links to video compression codecs we recommend Help gt Help install video compressors To save a video with compression select Video gt Use Video Compression and the following dialog box will pop up 32 List of Installed Compressors dialog box Select the desired compressor from the drop down box The options listed in the configure dialog box will depend on the installed compressor Questions regarding specific compressor settings should be directed to the compressor distributor 5 2 Recording speed A standard monochrome RS 170 video signal con
24. es several options for false color display for the 8 bit black and white images e Fluorescence Color Scheme e Spectrum Color Scheme e Interferogram Color Scheme e Food Coloring Color Scheme The reason for using false color display is that it is sometimes difficult to distinguish 256 different gray levels on a standard computer monitor Converting shades of gray into colors can significantly enhance visibility of small differences in gray scale value 30 Invert Color Scheme If checked the selected color scheme will be inverted i e Black and White Color Scheme will invert black and white pixels Like the false color schemes this option is used to enhance visibility Record in False Color Scheme If checked videos or snap shots will be recorded in the selected false color scheme If not checked the false color scheme will be used for viewing only and the video will be recorded in standard black and white 31 5 VIDEO RECORDING One of the main features of the uScope application is the ability to record long unbroken video sequences without compression These video sequences are stored in standard AVI format so that they can later be viewed by Windows Media Player or other video playback software off line processed by uScope or other video processing software compatible with the AVI standard To enable uncompressed video recording uScope makes use of a double buffering system described below The buffering scheme also enabl
25. evel adjust 9 Do not strobe O None Strobe illuminator LED Settings O Auto set image min to black Poststrabe delay 155 Manual O of 255 a m before recording ki Averaging White level adjust O None 9 Do not average 9 Auto set image max to white Manual 255 of 255 C Average 10 frames C Average for 1000 ms Image file naming Snap Shot Settings dialog box 36 Averaging Options include Do Not Average Snap shot will only include one frame Use this setting if you have motion and are trying to capture particle images Average x frames Number of consecutive frames averaged to form a single snap shot Average for x ms Number of ms averaged to form a single snap shot If you have a little to no motion increasing the number of frames or time will improve your signal to noise ratio for static images the signal to noise ratio will improve by approximately the square root of the number of frames averaged Black and White level adjust Use these values to change limits of the video spectrum In the full color spectrum 0 represents black and 255 represents white If this range is reduced everything below the set black level will appear black and everything above the set white level will appear white None No black or white level adjust Auto image minimum is set to black maximum set to white Manual set minimums black and maximum white to desired level 37 Cc Mie
26. ge Down keys on the keyboard Focus la a HS Illumination The LED illuminator channels strobe during each image frame The Illuminator control is used to adjust the strobe duration of each channel effectively altering the LED intensity For certain applications it may be useful to temporarily reduce the motion limits of the x y or focus The motion range can be reduced by selecting SVM gt Motion Limits from the main menu Modification of these limits may be useful if you have installed a device on the SVM i e a chipholder that may interfere with the stage at its preset limits The channels can be adjusted using the controls along the top of the main window or by pulling up the LED Pulse Durations window SVM gt LED Settings Illuminator Gang Al pj 5b ci D 22 uScope main window controls 6425 9197 2646 16000 Cancel LED Pulse Durations dialog box When an EPI optics module is installed the forth slider controls the EPI illumination and channel D is controlled with the Channel B slider button The maximum time duration that can be entered for the pulse duration is dependent on the camera frame rate and is equal to 1 f Checking the Gang box will cause all LED banks to be adjusted simultaneously when one slider is activated Note The Illuminator scroll bars do not update when the illumination is changed via the SVM front panel If the front panel will be used for illuminator co
27. ground could delete active particles Deconvolve Autocorrelation is an advanced option for high precision measurements This option deconvolves the cross correlation by the autocorrelation which can remove the effects of blur and particle size such that each particle is treated as a single point It is most useful when the signal to noise ratio is extremely high 5 The Show Field options control which data are displayed for each probe window a Cross correlation determines how far the particles move between frames or between every few frames based on the Frames Skipped option This field is a good diagnostic tool to help you optimize experiment parameters The red dot will move further from the center as the flow velocity increases The dot should be small and well defined to achieve the most reliable measurements If the flow is too fast the red dot will move outside of the window and uScope will not be able to measure the velocity In this case increase the window size which will improve the signal to noise ratio Autocorrelation is an indicator of resolution The mass at the center of the window will become sharper with smaller particles and better focus Mean shows the data that is being subtracted as part of the Background based upon the Background described above Showing the Mean can be helpful for highlighting stuck particles and other anomalies in the flow RMS is an indicator of the amount of useful signal available
28. led fixed filters that cannot be removed 12 2 5 Base stand Preset Lock indicator selectors LED Keypad Site light Toggle buttons Focus control SVM340 front panel 2 6 Front panel controls Many SVM340 features are accessible through the uScope software will go through the uScope application software You can also however access some important functions directly from the front panel The Power button and Power LED are located in the upper left corner of the front panel When the power is turned on the LED will flash green and red while the system runs its initial tests and turn green when the tests have passed Site These buttons toggle between SITE mode and LIGHT mode Light When the SITE button is lit the four storage buttons A D represent four different stored positions and the keypad controls the traverse movements The focus buttons moves the objective up and down for focusing When the LIGHT button is lit buttons A D represent the four 13 Keypad 14 LED channels and the keypad up and down buttons control the intensity of the LED banks selected by the A D buttons In SITE mode the buttons A D represent four stored settings of the traverse and focus positions and LED intensities To recall a stored position select SITE mode and press and release a storage button The traverse will move to the location and set the four LED intensities to the values of the stored settings To
29. nt replaces the Channel illuminator D adjustment slider button when an EPI module is turned off installed Channel D illumination control is ganged with channel B Color Imaging go to Video gt Color Format gt and ensure 24 bit camera settings mis RGB is selected images are adjusted in B amp W go to VideosProperties5 Video Proc Amp and maximize the Saturation setting Adjust 58 Brightness Contrast and Hue to optimize the image Error Message Failed to connect to LUT converter and or Cannot Give Graph to Builder incompatible video device selected or switching between video capture devices If an incompatible video capture source is selected such as VDP source or if you switch between capture sources you may get errors even when you go back and choose the correct source Shut down the uScope software turn off the SVM and then restart the SVM and software and select the correct source If the errors still persist go to Video gt Image Size and Format and select 720 x 480 for Output Size 59 7 3 Video and image quality Module B amp W EPI Color B amp W B amp W Color Color Format B amp Wor B amp Wor 24 bit RGB B amp W or B amp W or 24 bit RGB Fluor Fluor Fluor Fluor Video gt Brightness Contrast Hue etc Brightness adjust adjust adjust adjust adjust adjust typ low typ low typ low typ low Contrast adjust adjust adjust adjust
30. ntrol we recommend first setting all illuminator slider bars to the maximum setting to allow the full range of illuminator control More details on illuminator timing can be found in the Video and Illumination Timing Section below Presets The presets panel allow saving and restoring up to ten traverse x y and focus positions and illumination settings in storage cells A J Clicking a 23 button will load the stored preset and adjust traverse position and LED illumination to the stored values Checking the Save box first will store the current setting in the selected storage cell Sites A D can also be saved and accessed from the SVM front panel Presets occ AA a Ca Gd Ca a x To stop the traverse click the Stop Motion button x or the center button on the front panel keypad the SITE button must first be illuminated For descriptions of video recording and probe functions see sections 5 and 6 below 3 1 usc files The uScope software saves the instrument and video settings in a file with usc extension To open a uScope file choose File gt Open then locate the file on your hard drive You can also choose from recently opened files at the bottom of the File menu 3 2 Online and offline operation uScope can work in both online and off line mode When on line it communicates with a SVM340 controls its functions and accepts live video signals from a DirectX compliant video capture card When off
31. ons Opening Camera module RS 170 BW RS 170 C Objectives Illuminator modules Inputs Outputs Communication Physical Dimensions Weight Power 62 x 50mm y 75 mm focus 4mm x and y 10 um focus z 1 um X x Y 140mm x 200mm 55 x 80 mm Analog interlaced monochrome camera with 1 3 CCD 640 x 480 pixels 30 frames s Bayer pattern analog color camera with 1 3 CCD 640 x 480 pixels 30 frames s 10x plan 0 25 170 4x 20x LED B 3 blue 460 nm bandwidth 50 nm one white bank LED G 3 green 560 nm bandwidth 50 nm one white bank LED R 3 red 660 nm bandwidth 50 nm one white bank LED W 4 white banks LED X 1 red 1 green 1 blue 1 white bank 4 programmable digital inputs TTL level Composite analog video out S video out 3 programmable digital outputs TTL level 4 external illuminator trigger drivers TTL level Serial RS232 9 pin D sub connector W x L x H 208 x 267 x 85 mm 2 8 kg 90 240 VAC 47 63 Hz 100 VA
32. options set up sseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeneeees 26 4 2 VIDEO AND ILLUMINATION TIMING cccccceeeeees 28 4 3 Color format S t Up cccssceseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeneees 29 5 VIDEO RECORDING wicca 32 5 1 Video compression Naan 32 5 2 Recording Speed mama a aaa 33 5 3 Buffering see aaa KAKAA KUWAKA WA KAWA KUWA KUWAKA WA KUWA KUWa 33 54 Pre and post trigger recording mmmmmmmmmmzi 35 5 5 Recording a VIGCO cceeeeseeseeeeeeeeeeeeeseneeneeeeeeeeeeeeenenees 35 5 6 Snap shot SettinGS scsssseeeeeeeeeeeesseeeeeeeeeeeeeeeeeeeeeees 36 5 7 Deinteracing ie cases ete duicatete deca tata deca atte rena deca teenies eats 39 5 0 Time LAP SO ia 42 5 9 Distance INGICALON kama AK NA NAANAAGAAAA 43 B PROBES Sama Aa 45 6 1 Velocity PIV probes cccccceesssssseeeeeeeeeeeeesenseeeeeees 45 6 2 Polygon and Macropixel intensity probes 49 6 3 Recording probe data sssssesssssssessesssssseeees 53 6 4 SAVING probes www wina uawa wakikiuka wakakaa 55 7 TROUBLESHOOTING ois aakala 56 7A Getting help SE oinsianaiini isini aii ainiaan 56 7 2 Video imaging iia KUAKAA 57 7 3 Video and image quality ccccccssssesseeeeeeeeeeeeseeees 60 7 4 Dropped frames cccccceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeseeeeeeess 61 SPECIFICATIONS 2am aa AA 62 1 INTRODUCTION The SVM340 is an inverted fluorescence microscope with built in
33. ors without S video input The four digital inputs and three outputs on BNC connectors provide TTL level communication with external equipment The inputs can be used to control or strobe the four LED illuminator channels or to trigger more advanced behaviors The outputs are selectable and include video timing information motion status information and several advanced programmable flags The inputs and outputs are set from the menu SVM gt Digital Outputs amp Inputs Out2 9 Video field sync 9 Motion status O Video composite sync Sequence F3 Sequence F1 Camera trigger out O Sequence F2 Sequence F4 Out is high during odd fields Out2 is high when the SVM is and low during even fields moving and low otherwise transition on vertical sync Illuminator strobe gate source Channel A 9 Internal Ind Channel B 9 Internal InB Channel C O Internal Inc Channel D 9 Internal InD An illuminator channel strobed from an external input is lit while the inputis high Disconnected external inputs are high Cancel Digital Output and Input Window External Illuminator The inputs could be connected to digital experiment controllers like the LabSmith LC880 interlock switches sensors or other external devices and the outputs connected to other apparatus to facilitate real time control and automation This male nine pin D sub connector provides 5 V DC power and pulse signals to drive 4 external illumination sources with se
34. ppend counter starting at 10 Example C Users Eric Cummings Documents uS cope Files SVMO00010 jpg Naming settings for snap shots 5 7 Deinterlacing The camera built into the SVM340 runs in standard RS 170 monochrome or NTSC color video format at a fundamental frame rate of 30 Hz As discussed in section 4 1 the full video frame is composed of two interlaced fields at a field frequency of 60 Hz This means that every second line of a full image is recorded at a time 16 6 ms later than the other half of the lines As a 39 consequence fast moving objects will be recorded with a slight horizontal blur which is caused by the image segment in the even lines being shifted slightly from the image segment in the odd lines Image of a horizontally moving particle recorded with the SVM340 interlace camera Zooming in on such a fast moving particle reveals the jagged edges caused by the interlace camera format To reduce the effects of the interlacing uScope includes deinterlace filters which will reduce the visual appearance of the blurring caused by the interlacing by various algorithms The delinterlace method is selected in the Video gt Deinterlace Video options dialog box 40 Deinterlacing algorithm Do not deinterlace O Two frame Bob Blended clipping O Weave Field Bob If you experience horizontal combs in moving parts of your images your video file or source is interlaced Select a deinte
35. r bar between now and the maximum buffer size This sets the time duration recorded from the pre trigger buffer I IL 9 Unlimited Gag 0 sago s Emig v Advanced U U Then either set the For Duration time or leave it as Unlimited The duration time represent the total video duration pre trigger buffer post trigger record time If Unlimited video duration is used the video will record until the record button is pressed again The total duration of the video will be the buffer time plus the newly recorded time the time the video button is depressed f Recordfram 7ng pa naw For duration ig 1000s Note uScope software now uses buffering for the video recorded live so that this video is also saved without dropping frames 35 5 6 Snap shot settings A snap shot is a single image consisting of an average of one or more consecutive images Snap shots are taking by pressing the or C73 button both located in the lower left hand corner of the main window Takes a snap shot using the predetermined frame averaging and other options specified in the Snap Shot Settings see below C7 Takes a snap shot and frame averages until the button is pressed again All other options specified in the Snap Shot Settings other than Number of frames to average are maintained Snap Shot Settings The dialog box is opened from Views Snap Shot Settings Flash Settings applied only to live video Black l
36. ral visual imagery and may not be suitable for all types of imagery occurring in microfluidics device diagnostics e g the images of isolated small particles as recorded in Particle Image Velocimetry PIV experiments The ability to record uncompressed video is therefore an important feature of the hardware and software included with the SVM340 Uncompressed video streams naturally take up more bandwidth and use more computer processing power for display and storage so a powerful computer is recommended When used on a newer standard PC with moderately fast CPU and disk speed uncompressed video sequences can usually be stored on disk in real time If used on slower computers frames may be lost during recording Slower computers may also exhibit a perceptible delay between an imaged event and its appearance on the computer display Also a large hard disk is recommended for storage of video data A color video signal will typically generate 1 6 GB per minute and thus quickly consume hard disk space 1 5 Installing the software 1 5 1 Installing the video capture card and or drivers A video capture card or peripheral USB capture device is required to view and record the video signal on your computer If you purchased one of the devices through LabSmith a separate instruction manual with the installation instructions and driver disk will be included with the device It is always a good idea to check with the manufacturer s website to make
37. rlacer to mitigate this artifact If your video is not interlaced select Do not deinterlace 7 Odd field is first recommended An interlaced frame consists of alternating horizontal stripes of video taken at half the frame period apart Most cameras including those of the SYM present the earlier image in the odd lines field Live Video Deinterlace Video options dialog box The best deinterlace filter depends on the nature of the video image and should be chosen by experimentation A brief description of the three most common algorithms is given here Weave This method uses three fields in the calculation and works well on slow moving material but tends to fail on fast moving material Bob The basic bob algorithm uses the most recent field and fills in the lines between by interpolation This method detects weaving artifacts in the current image it uses bob to get rid of them This method has a tendency to bob rather too much and gives poor results on fine static images Two frame This method uses the current frame and the last two to determine whether to bob or weave a given pixel This gives better results on both stationary and moving images than the above two methods but uses more CPU 41 Although the deinterlace filters improve the visual appearance of the video they are not always effective for scientific imagery All filters are based on some form of interpolation between frames under the assumption that sc
38. roduce a sample of polystyrene fluorescent particles 4 Adjust focus illumination and flow characteristics Alternately you can also open an existing flow movie by choosing Video gt Process Saved Video Creating velocity probes 6 1 2 Creating a PIV probe To create a probe 1 Click the Velocity Probe toolbar button Ry to highlight the button 2 With your mouse curser over the image right click on the mouse and select New Probe Bring the mouse to the center point for the new 45 probe and left click the mouse to place the probe A probe such as the one below will appear New probe showing correlation field vector arrow and real time velocity as text 3 Repeat step 2 to create as many probes as desired Once created the probes can be moved by clicking and holding the mouse button over the probe and dragging it to the desired location 5 Click the and toolbar buttons to make the probes more or less transparent The software will continue to calculate velocity at these locations even if you make the probes completely invisible 6 To remove a probe right click on it and choose Delete When you first create a probe uScope will experimentally determine the fastest FFT algorithm to use This process may take up to 60 seconds to complete at which point the software will begin calculating velocity at the location When you create a probe it will take on the properties of the last probe you altered Once created you
39. s module Note When removing or inserting the optics module take care not to apply excessive force since this may damage the traverse mechanism and compromise traverse accuracy 2 3 Microscope objective The microscope objective is a standard DIN type objective with 160 mm conjugate image distance To replace the objective remove the camera module and unscrew the objective The SVM340 supports objectives with magnifications from 4x to 20x Objectives with higher magnification generally have insufficient stand off distance to clear the illuminator LED s and can only be used with an external illuminator module or other external light source WARNING Dust particles can enter the optics module when the objective is not installed Removal or changing of the objective should be done in a clean environment when possible We recommend 11 that a piece of tape be placed over the objective opening if the optics module will be stored without the objective installed Fluorescence filter installed in an optics module note filter washer is placed on top of filter 2 4 Fluorescence filter The fluorescence filter can be installed in the B amp W and Color camera modules The filter is installed by removing the objective and carefully placing the filter in the recessed opening A compliant washer provided with the filter is placed over the filter to keep it secure under the objective Note The EPI camera module is supplied with pre instal
40. secutive fields may appear jagged at the horizontal edges See section below for a discussion of the implications and tools to control the interlacing effect 4 3 Color format set up The SVM340 can be fitted with either a grayscale B amp W or color camera module The uScope software provides many options for viewing and recording images 29 File Edit View SVM Help acl Playback Process Saved Video Focus Presets o Al Capture Process Live Video E Save Record Pause Stop Recording Use video compression Color Format 24 bit RGB true color Video Capture Device Black and White Color Scheme Brightness Contrast Hue etc Fluorescence Color Scheme Image Size and Format Spectrum Color Scheme Deinterlace Video Interferogram Color Scheme Frame Rate Food coloring Color Scheme Physical Input Connector Invert Color Scheme Record in False Color Scheme uScope video imaging options 24 bit RGB true color Used for true color images The user should note that color video data takes up 8 bits each for the red blue and green colors and thus consumes three times as much disk space and processing time as the BAW video If color images are not needed the color optics module can be used in B amp W mode to save disk space Black and White Color Scheme 8 bit greyscale images provides 256 gray scale levels False Color Display The uScope software also provid
41. sition and external devices in response to up to four trigger input signals In each of these modes you can use microscope objectives with magnification from 4X to 20X and acquire and store the video output on standard analog video storage hardware With the uScope application you can directly store the video data on computer disk as avi files and perform advanced real time video processing 1 4 Computer requirements The uScope software is designed to stream digitized video sequences directly to disk so it is recommended that the computer fulfills the following minimum requirements e Windows XP Vista or Windows 7 or newer e USB2 0 port for the external video capture device or a PCI slot for the internal video capture card e RS232 port for communication or USB2 0 port and a RS232 USB adapter e minimum of GB of RAM 4 8 GB recommended e 80 GB hard disk with 12 ms access time 500 1000 GB recommended The SVM340 microscope includes a VGA resolution analog CCD camera which outputs a standard RS170 monochrome or NTSC color composite video signal The video signal is digitized by a DirectX9 0 compliant PCI video capture card or USB2 0 video capture peripheral capable of digitizing and storing uncompressed VGA resolution video on disk Many PC video input devices include on board image compression hardware converting the video stream into various compressed video formats Image compression standards like MPEG are designed for gene
42. tal x and vertical y direction of each sub probe and the selected frame averaging n 54 The probe data column headings a b are the position of the sub probe in the array horizontal vertical with the bottom left corner sub probe listed as position 0 O 6 4 Saving probes A set of probes can be saved to disk and recalled later 1 Arrange the probes and set their Properties pa Choose File gt Save Probes As to create a new probe file Probe files are saved in the uScope file formate usc 3 Select the name and location for the file and click Save To recall a stored set of probes choose File 5 Open 55 7 TROUBLESHOOTING 7 1 Getting help This guide is your main source for information on operating the SVM340 and the uScope software Check the LabSmith web site www labsmith com for user manual updates application notes and information to help you use the SVM340 If you are unable to find the help you need call the LabSmith technical support at 925 292 5161 or send an e mail to support labsmith com If you need support please write down the serial number of your SVM340 located on the bottom of the unit and the version of the software you are using To get the software version number click Help About uScope in the uScope main window 56 7 2 Video imaging Problem Possible Resolution Cause no video problem with A video capture device is required to image video signal video capture
43. tart recording until the event has been observed The duration of the buffered video sequence depends on the amount RAM set aside for the buffer The more RAM installed in the computer the more buffer space can be set aside without slowing down other tasks The initial buffer size is set to half the available RAM but you may want to adjust the buffer size get longer pre trigger video sequence duration Buffer size is specified in The View gt Video Buffering settings menu RAM buffer Percentage of available RAM Video Buffer Settings dialog box Note Choosing the 24 bit option for recording video Video gt Color Format gt 24 bit RGB true color requires 3 times the buffer RAM of all other video options 34 5 4 Pre and post trigger recording The figure below illustrates the effect of the buffer size on the pre and post trigger recording durations Trigger Stored video Time RAM Buffer Stored video 7 ime 4 DIN go Disk Buffer Pre trigger top and post trigger bottom recording For pre trigger recording you save the video sequence occurring before the trigger stored in the RAM buffer With post trigger recording you save the video sequence occurring immediately after the trigger and temporarily stored in the disk buffer The disk buffer is normally larger than the RAM buffer 5 5 Recording a video To record a video set the time in the Record from box to the desired number of seconds or drag the slide
44. ttings similar to the four bank led module Pin connections 1 5 V DC max 3 A 2 Chassis Ground 0 V 3 Chassis Ground 4 Chassis Ground 5 Chassis Ground 6 LED A drive TTL 7 LEDB drive TTL 8 LED C drive TTL 9 LED D drive TTL The light intensity of the SVM340 LEDs is controlled by pulse width modulation with a frequency synchronized to the video signal Full light intensity means an illumination duty cycle close to 100 LED drive outputs A D are negative logic i e TTL level is high when LEDs are off 2 8 Microscope stage The SVM340 is fitted with a replaceable microscope stage attached to the main body of the instrument by four magnetic locks To remove the stage simply pull the stage gently up until it releases The stage top is a polished stainless steel plate which can be machined to provide application specific mounts for the fluidic device electrodes fluid hoses or other fittings 17 7 SVM34O syocrosired 140 Microscopi D Removing the microscope stage LabSmith also offers an integrating sample stage A SVM Stage BB that can be used to couple the SVM340 microscope to one of LabSmith s breadboards iBB or LS600 CH iBB mounting holes LS600 CH mounting holes Integrating Delrin sample stage 18 The breadboard is mounted onto four screws installed on the Delrin stage The inner stage mounting holes are used for the LS600 CH
45. tton over the probe and dragging it to the desired location The shape of the probe can also be modified by dragging the polygon points to create the desired shape Intensity probe data displayed and recorded can be selected by selecting the probe left click then right click and select Properties The following dialog box will appear Display options Red Green Blue Average Spatial RMS Saturation Maximum F Minimum Cancel Triggering Polygon Probe Properties dialog box For images from the B amp W or EPI optics module only the Gray displays are applicable 6 2 2 Macropixel intensity probe properties Macropixel intensity probes are used when spatially resolved intensity data is needed The probe is divided into an array of sub probe sections based on the input pixel size To create a probe 1 Click on the macropixel button on the toolbar to highlight the button 2 Right click on the image and select insert new probe 3 Double click where you would like to place the probe The probe can be dragged on the screen to a new position or resized by dragging a comer 4 Right click on the probe and select Properties brings up the following window 51 Macropixel size Horizontal 8 x image Vertical 8 v image E Time average by time constant 10 v Overlay image Macropixel Probe Properties Changing the macropixel size ch
46. verage of one or more consecutive images Motion Limits Motion limits on the XY stage are useful if you have installed a device on the SVM i e a chipholder that may interfere with the stage at its preset limits Video compression Though uScope does not have built in compression it can now use video compressors that are installed on your computer To add compression functionality you need to download and install a codec A compatible codec will also need to be installed on anything that plays the video Deinterlacing Improvements have been made to the deinterlacing method and to the Deinterlace Settings dialog box Buffering Improvements have been made to the method by which video is buffered to RAM memory The newest version of uScope software uses video buffering for the video recorded live not from the pre trigger buffer so that this video is also saved without dropping frames Recording a video Improvements have been made to the interface for recording pre and post trigger video Distance indicator The Distance Indicator allows you to measure features on your image 1 2 Package contents Stage Plate Stage Illuminator Microscope Body Objective Schott Filter Camera Optics Module The basic SVM340 includes the following items Microscope body 4 standoffs feet B amp W optics module 4 channel illuminator typically Blue 10X objective Delrin sample stage standard stage plate 50 mm
47. verts into a digital data rate of 8 9 MBytes s which can easily be read into PC RAM memory in real time It is also within the capability of modern fast computers to write to hard disk in real time at this data rate provided the computer is not overloaded by simultaneously executing other disk or CPU intensive tasks Color NTSC video signals convert into a data rate of 26 4 MBytes s which can readily be written to RAM memory in real time but may be too high to write to hard disk in real time In that case some frames scattered throughout the video sequence are lost resulting in a stored video sequence with time intervals of 33 3 ms between most images but with 66 6 ms 99 9 ms or some other multiple of the base frame interval between some individual images Such lost frames are called dropped frames Video sequences with dropped frames are not suitable for accurate time history analysis since it is difficult to know afterwards exactly where frames are missing 5 3 Buffering The SVM s uScope software uses buffering to capture images without dropping frames uScope stores digitized video date in a cyclic RAM buffer simultaneous with the display Buffering also allows the user to selectively record events after they occur For instance to catch a particular particle or 33 droplet passing through the field of view the user can set uScope to start recording from a number of seconds before the record button is pressed and then wait to s
48. video camera fluorescence filter pulsed Light Emitting Diode LED illuminator motorized x y traverse and focusing actuator It can directly image fluorescent or non fluorescent samples on a standard video monitor or video recorder In addition the SVM340 includes an advanced programmable synchronization unit with four inputs and three outputs for synchronizing image acquisition to external events The basic functions can be controlled by the front panel controls or through the uScope application software included with the instrument 1 1 NewinuScope Version 1 04 Version 1 04 of uScope software includes a number of new features and improvements to existing features Macropixel intensity probes The macropixel probe is a rectangular intensity probe that can be subdivided into discrete sections for spatially resolved intensity measurements Expanded Time lapse options The Time lapse option lets you select the frame rate frequency to allow video taken over a long period of time to be compressed to a small file and played back at increased speed The latest version of uScope also allows you to apply snap shot settings i e flash settings averaging black and white level adjust to the time lapsed video essentially compiling a series of snap shots into a time lapsed video Some other relatively new features implemented in v1 026 include Snap shot settings The snap shot feature lets you create a single image consisting of an a
49. with an analog camera optics module A RS170 image does device BW A RS170 Color or A EPI Ensure the not change correct capture device has been selected go to when the Video gt Video Capture Device illumination e StarTech capture device SVID2USB2 is listed is changed as WDM2821 or your e Imaging Source capture device DFG USB2 hand is PRO is called DFG USB2pro waved over e Hauppauge internal video card is called the video Hauppauge WinTV Capture If your video capture device is not listed in the Detected Video Capture Hardware Window ensure that the device drivers are correctly installed and the capture device is plugged into a USB port on the computer s video cable not installed S Video cable must be installed in the back of the SVM and in the Video Adapter Check each end of the cable to ensure the 4 pins of the connector are intact S video input not selected optics module installed incorrectly go to Video gt Physical Input Connector and select S video for the input The optics module has two pins to guide it into place and is held to the SVM focus stage with magnets Ensure that the module is squarely in place on the focus stage and that magnets are engaged not focused on target or target not properly illuminated If the optics module is not focused on an object it sometimes appears that you are not getting a signal This is especially true with the EPI module or a standard mo
50. y result in loss of communication with the instrument that requires LabSmith assistance to resolve 25 4 uSCOPE VIDEO PLAYBACK AND PROCESSING When the uScope software is opened the main window below will appear To playback or process a saved video open the AVI file you want to view To capture or process a live video click cancel in the Open dialog box and then click Video gt Capture Process Live Video 4 1 Video options set up The uScope software will attempt to connect to the most recently used capture device If that device is not found the Detected Video Capture Hardware window will pop up Or select Video gt Video Capture Device to open this window 26 Detected Video Capture Hardware paa Integrated Webcam WDM 2820 Capture Paint Grey Camera VDP Source Ifyou do not see your video capture device listed here please ensure that your capture device is plugged in to your computer usually via a USB or PCI connection Also ensure that your video capture device has been installed correctly Close and re open this dialog to refresh the device list Ca Click on the appropriate video capture device Video capture devices sold by LabSmith include e StarTech capture device SVID2USB2 is listed as WDM2821 e Imaging Source capture device DFG USB2 PRO is listed as DFG USB2pro e Hauppauge internal video card is listed as Hauppauge WinTV Capture Note If your video
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