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Agilent 1260 Infinity Bio-inert Manual Injector
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1. o ooo ooo ao v Q o Figure 4 Installing the System 16 Bio inert Manual Injector Installing the ManualInjector 2 Installing the Manual Injector _ TI APG remote connector Position sensor cable Figure 5 Installing the Start Cable 6 Connect the capillaries to the manual injector see Flow Connections on page 18 Bio inert Manual Injector 17 2 Installing the Manual Injector Flow Connections WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Connect the pump outlet capillary to port 5 2 Connect the column compartment inlet capillary to port 4 3 Connect the sample loop between ports 3 and 6 Needle port Waste to column from pump Sample loop Figure 6 LOAD Position 18 Bio inert Manual Injector Installing the ManualInjector 2 Leak Drainage Leak Dra
2. Agilent 1260 Infinity Bio inert Manual Injector User Manual Notices O Agilent Technologies Inc 2011 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 65628 90000 Rev Edition 05 12 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agil
3. Bio inert Manual Injector 1535 4045 5068 0082 0100 1851 5068 0060 1535 4857 5067 4158 Description Bearing Ring Oty 1 replacement Rotor seal PEEK Stator face ceramic Bio inert stator head Stator screws 10 Pk Bio inert 6 port sample injection valve Injection Valve Assembly 41 5 Sample Loops PEEK 42 Parts and Materials for Maintenance p n 0101 0101 0101 0101 0101 0101 0101 0101 0101 0101 1241 1240 1239 1238 1242 1227 1236 1235 1234 1230 Description Sample loop 5 uL Sample loop 10 uL Sample loop 20 uL Sample loop 50 uL Sample loop 100 pL Sample loop 200 uL Sample loop 500 uL Sample loop 1 mL Sample loop 2 mL Sample loop 5 mL Bio inert Manual Injector Syringes p n 5190 1506 5190 1513 5190 1527 5190 1534 5190 1457 Bio inert Manual Injector Description Syringe 50 PTFE FN luer lock Syringe 100 pL PTFE FN luer lock Syringe 500 pL PTFE FN luer lock Syringe 2 5 mL PTFE FN luer lock Syringe 10 mL PTFE FN luer lock Parts and Materials for Maintenance 5 43 5 Parts and Materials for Maintenance Accessories and Consumables 44 p n 5190 0924 5062 8541 5188 6593 9301 1377 5188 2758 9301 1379 G5667 60501 Description PEEK luer lock needle AY PEEK Fittings 10 PK Snap vial 2 mL fixed insert clear 100 pk Screw Cap Vial clear 6 mL 100 PK PTFE silicone septa 16mm pre silt 100 pk Screw caps for 6 mL vials 100
4. Injector Using the Manual Injector 3 PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability and is therefore the material of choice for biochemical instrumentation It is stable in the specified pH range and inert to many common solvents There is still a number of known incompatibilities to chemicals like chloroform methylene chloride THF DMSO strong acids nitric acid gt 10 sulphuric acid gt 10 sulfonic acids trichloroacetic acid halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc When used above room temperature it is sensitive to bases and various organic solvents which can cause PEEK to swell As normal PEEK capillaries are very sensitive to high pressure especially under such conditions Agilent uses stainless steel cladded PEEK capillaries in order to keep the flow path free of steel and to ensure pressure stability to at least 600 bar In case of doubt please consult common literature about the chemical compatibility of PEEK Titanium Titanium is highly resistant to oxidizing acids e g nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures which is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Reducing acids e g hydrochloric sulfuric and phosphoric acid can slightly corrode titanium which inc
5. PK PEEK SST cap 0 17 mm ID 500 mm long Bio inert Manual Injector Bio inert Manual Injector ptem Agilent Technologies on Internet Additional information 46 Agilent Technologies 45 6 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the internet at http www chem agilent com 46 Bio inert Manual Injector Index Index A Agilent on internet 46 B bio inert 31 materials 8 buffers 33 C cleaning the manual injector 34 D damaged packaging 12 delivery checklist 13 E exchanging the rotor seal 35 exchanging the stator face 37 F flow connections 18 flushing the manual injector 33 information on internet 46 INJECT 25 installation 14 installing the manual injector 14 internet 46 46 Bio inert Manual Injector L leak channel 19 worldwide web 46 leak drainage 19 LOAD 24 M materials bio inert 8 needle port cleaner 33 P precision 24 rotor seal 35 S salt solutions 33 sample volume 24 solvent information 26 solvents buffers 33 rinsing 33 salt solutions 33 stator face 37 U unpacking damaged packaging 12 delivery checklist 12 47 www agilent com In This Book This manual covers the Agilent 1260 Infinity Bio inert Manual Injector G5628 This manual contains user information about the Agilent 1260 Infinity Bio inert Manual Injector The manual describes the
6. Switch the valve to the INJECT position 2 Use the pump to flush the sample loop and seal grooves Bio inert Manual Injector 33 4 Maintenance Cleaning the Manual Injector The manual injector base should be kept clean Cleaning should be done with soft cloth slightly dampened with water or a solution of water and a mild detergent 34 Bio inert Manual Injector Maintenance 4 Exchanging the Injection Valve Seal When Poor injection volume reproducibility Leaking injection valve Tools required Description Hexagonal key 9 64 inch Parts required p n Description 5068 0082 Rotor seal PEEK 1 Loosen the three stator screws and remove the stator 2 Remove the stator head and stator face head Bio inert Manual Injector 35 4 Maintenance Exchanging the Injection Valve Seal 3 Remove the stator ring and rotor seal 4 Install the new rotor seal HINT Beware of correct orientation The rotor seal grooves must be visible 5 Install the stator ring stator head and stator face onto the 6 Secure stator head in place with the stator screws valve Tighten each screw alternately 4 turn until the stator head is secure 36 Bio inert Manual Injector Maintenance 4 Exchanging the injection valve stator face When Tools required When visibly scratched or when the valve performance shows indication of leakage or wear Description Hexagonal key 9 64 inch Parts require
7. following Introduction installing the manual injector using the manual injector maintenance of the manual injector parts and materials and additional information O Agilent Technologies 2011 2012 Printed in Germany 05 12 G5628 90000 Rev B RES Agilent Technologies
8. inert 60 mm V o 4 0 pl p n G5615 60017 for Agilent 1290 Infinity Diode Array Detectors DAD G4212A B Bio inert flow cell 8 pL 20 bar pH 1 12 includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Bio inert heat exchanger G5616 60050 for 1290 Infinity Thermostatted Column Compartment G1316C Bio inert Valve heads Bio inert Connection capillaries PEEK zirconium oxide sapphire PTFE PEEK fused silica PEEK fused silica PTFE PEEK steel cladded G4235A G5631A G5639A PEEK ceramic 1505 based Upstream of sample introduction Titanium Downstream of sample introduction Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar For ensuring optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see Solvent information for parts of the 1260 Infinity Bio inert LC system on page 26 Bio inert Manual Injector 1 Introduction Bio inert Materials 10 Bio inert Manual Injector Bio inert Manual Injector 2 Installing the Manual Injector Unpacking the Manual Injector 12 Damaged Packaging 12 Delivery Checklist 13 In
9. toxic or hazardous solvents are used Needle port Waste to column Sample loop 24 LOAD Position In the LOAD position see Figure 8 on page 24 the pump is connected directly to the column ports 4 and 5 connected and the needle port is connected to the sample loop At least 2 to 3 sample loop volumes more if better precision is required of sample should be injected through the needle port to provide good precision The sample fills the loop and excess sample is expelled through the waste tube connected to port 2 from pump Figure 8 LOAD Position Bio inert Manual Injector Using the Manual Injector 3 INJECT Position In the INJECT position see Figure 9 on page 25 the pump is connected to the sample loop ports 5 and 6 connected of the sample is washed out of the loop onto the column The needle port is connected to the waste tube port 2 Needle port Waste to column from pump Sample loop Figure 9 INJECT Position Bio inert Manual Injector 25 3 Using the Manual Injector Solvent Information 26 Solvent Information Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see pump manuals Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents which may corrode parts in the flow path Consid
10. are no documented incompatibilities for HPLC applications PTFE PTFE polytetrafluorethen is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Sapphire Ruby and Al 03 based ceramics Sapphire Ruby and ceramics based on Al903 are inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Data above were collected from external resources and are meant as a reference Agilent cannot guarantee for the completeness and correctness of such information Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually increases at higher temperatures In case of doubt additional resources should be consulted Bio inert Manual Injector Bio inert Manual Injector 4 Maintenance Cautions and Warnings 30 Overview of Maintenance 32 Flushing the Manual Injector 33 Cleaning the Manual Injector 34 Exchanging the Injection Valve Seal 35 Exchanging the injection valve stator face 37 Instructions on simple routine repair procedures E Agilent Technologies 29 4 Maintenance Cautions and Warnings WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and rea
11. ction 6 o Bio inert Materials 8 Operation and mechanical hardware RE Agilent Technologies 1 Introduction Introduction The Agilent 1260 Infinity Bio inert Manual Injector G5628A can be used for manual operation or use of large injection volumes It offers a standard injection volume of 20 uL optional 5 uL to 5 mL and ensures highest injection accuracy It uses a Bio inert 6 port sample injection valve Sample is loaded into the external 20 uL sample loop through the injection port at the front of the valve The valve has a PEEK injection seal A make beforebreak passage in the stator ensures flow is not interrupted when the valve is switched between the INJECT and LOAD positions and back again The valve is mounted on a steel mounting pole and can be installed at the left or right hand side of the LC system Bio inert Manual Injector Introduction Introduction Mounting pole Injection valve 2 Podi Qo Waste vial 6 ml uu lt gt Syringe PTFE SE N gt with PEEK needle No PEEK sample loop 1 ME Sample vial 2 ml Valve handle Figure 1 Agilent 1260 Infinity Bio inert Manual Injector Bio inert Manual Injector 1 Introduction Bio inert Materials For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scienti
12. d p n Description 0100 1851 Stator Face 1 Loosen the three stator screws and remove the stator 2 Remove the stator head and stator face head Bio inert Manual Injector 37 4 Mai ntenance H the injection valve stator face angin 3 Insert the new stator face onto the stator head 4 Install the stator ring stator head and stator face onto the valve 5 Secure stator head in place with the stator screws Tighten each screw alternately 4 turn until the stator head is secure 38 Bio inert Manual Injector Bio inert Manual Injector 5 Parts and Materials for Maintenance Manual Injector 40 Bio Inert Manual Injection Valve Assembly 600 bar 41 Sample Loops PEEK 42 Syringes 43 Accessories and Consumables 44 Detailed illustrations and lists for identification of parts and materials RE Agilent Technologies 39 5 Parts and Materials for Maintenance Manual Injector Manual Injector Item p n Description 1 5067 4158 Bio inert 6 port sample injection valve 2 5001 3738 Mounting pole stainless steel 3 G1328 44111 Base plate 4 5042 8553 Organizer plate G5667 60501 PEEK SST cap 0 17 mm ID 500 mm long Manual Injector valve see Bio Inert Manual Injection Valve Assembly 600 bar on page 41 40 Bio inert Manual Injector Parts and Materials for Maintenance 5 Bio Inert Manual Injection Valve Assembly 600 bar Item p n 1 R wo N Figure 10
13. ent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered t
14. er specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Solvent information for parts of the 1260 Infinity Bio inert LC system For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials see Bio inert Materials on page 8 in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals like iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction does not contain metals at all However there are no materials which combine suitability for versatile HPLC instrumentation valves capillaries springs pump heads flow cells etc with complete compatibility to all possible chemicals and application conditions This section gives recommendations which solvents should be preferred Chemicals which are known to cause issues should be avoided or exposure should be minimized e g for short term cleaning procedures After using potentially aggressive chemicals the system should be flushed with compatible standard HPLC solvents Bio inert Manual
15. gents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice The volume of substances should be reduced to the minimum required for the analysis Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 30 Bio inert Manual Injector Maintenance 4 CAUTION Safety standards for external equipment gt f you connect external equipment to the instrument make sure that you only use accessory units tested and approved acco
16. inage WARNING Leaking injector fittings In the event of a leak solvent will drop into the leak channel in the baseplate from where it is channelled to the front and back of the baseplate gt Check the manual injector fittings periodically for signs of leakage Leak channel Figure 7 Leak Drainage Bio inert Manual Injector 19 2 Installing the Manual Injector Leak Drainage 20 Bio inert Manual Injector Bio inert Manual Injector 000 3 Using the Manual Injector e 6 e e e ji ij Injection Seal 22 Needles 23 Injecting Sample 24 Solvent Information 26 How to use the manual injector RE Agilent Technologies 21 3 Using the Manual Injector Injection Seal The Bio inert Manual Injector is supplied with a PEEK injection seal 22 Bio inert Manual Injector Needles CAUTION Needle can damage valve Always use the correct needle size Using the Manual Injector Needles 3 Bio inert Manual Injector 23 3 Using the Manual Injector Injecting Sample WARNING Ejection of mobile phase When using sample loops larger than 100 pl mobile phase may be ejected from the needle port as the mobile phase in the sample loop decompresses Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when
17. k p n 5190 1506 1 Sample loop 20 uL p n 0101 1239 1 PEEK luer lock needle AY p n 5190 0924 1 Agilent LC Hardware Documentation DVD p n G4800 64500 1 Bio inert Manual Injector 13 2 Installing the Manual Injector Installing the Manual Injector CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions The manual injector can be installed at the left or right hand side of the instrument stack 1 Place the baseplate on the bench 2 Connect the two organizer plates to the base plate Figure 2 Connecting the Organizer Plates 3 Screw the mounting pole into one of the two holes in the organizer plate 14 Bio inert Manual Injector Installing the Manual Injector 2 Installing the Manual Injector 4 Slide the manual injector onto the mounting pole Tighten the mounting screw Mounting screw Figure 3 Installing the Mounting Pole and Manual Injector Bio inert Manual Injector 15 2 Installing the Manual Injector Installing the Manual Injector 5 Install other system modules on top of the manual injector baseplate ge
18. o could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Bio inert Manual Injector Contents Contents Introduction 5 Introduction 6 Bio inert Materials 8 Installing the Manual Injector 11 Unpacking the Manual Injector 12 Installing the Manual Injector 14 Flow Connections 18 Leak Drainage 19 Using the Manual Injector 21 Injection Seal 22 Needles 23 Injecting Sample 24 Solvent Information 26 Maintenance 29 Cautions and Warnings 30 Overview of Maintenance 32 Flushing the Manual Injector 33 Cleaning the Manual Injector 34 Exchanging the Injection Valve Seal 35 Exchanging the injection valve stator face 37 Parts and Materials for Maintenance 39 Manual Injector 40 Bio Inert Manual Injection Valve Assembly 600 bar 41 Sample Loops PEEK 42 Syringes 43 Accessories and Consumables 44 Appendix 45 Agilent Technologies on Internet 46 Bio inert Manual Injector Contents 4 Bio inert Manual Injector Bio inert Manual Injector 000 1 659 Introduction e e E e e e e pi e Introdu
19. rding to the safety standards appropriate for the type of external equipment CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system Bio inert Manual Injector 31 4 Maintenance Overview of Maintenance Table3 Overview of Maintenance Procedures Procedure Typical Frequency Time Required Flushing the Manual After using aqueous buffers or 5 minutes Injector on page 33 salt solutions Exchanging the Injection After approximately 10000 to 10 minutes Valve Seal on page 35 20000 injections or when the valve performance shows indication of leakage or wear Exchanging the injection valve When visibly scratched or 10 minutes stator face on page 37 when the valve performance shows indication of leakage or wear 32 Bio inert Manual Injector Maintenance 4 Flushing the Manual Injector CAUTION Damage through crystal formation The use of aqueous buffers or salt solutions can lead to crystal formation which may cause scratches on the injection seal gt Always rinse the valve with water after using aqueous buffers or salt solutions 1
20. reases with acid concentration and temperature For example the corrosion rate of 3 HCl about pH 0 1 at room temperature is about 18 um year At room temperature titanium is resistant to concentrations of about 5 6 sulfuric acid about pH 0 3 The addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is subject to corrosion in anhydrous methanol which can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 26 Fused silica Fused silica is inert against all common solvents and acids except hydrofluoric acid It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH larger than 12 the use of flow cells with sapphire windows is recommended Bio inert Manual Injector 27 3 28 Using the Manual Injector Gold Gold is inert against all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia a mixture of concentrated hydrochloric and nitric acid Zirconium Oxide Zirconium Oxide ZrO is inert against almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Platinum Iridium Platinum Iridium is inert against almost all common acids bases and solvents There
21. stalling the Manual Injector 14 Flow Connections 18 Leak Drainage 19 Installation of the manual injector RE Agilent Technologies 2 Installing the Manual Injector Unpacking the Manual Injector 12 Damaged Packaging Upon receipt of your manual injector inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the manual injector has been mechanically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection Bio inert Manual Injector Installing the Manual Injector 2 Delivery Checklist Ensure all parts and materials have been delivered with the manual injector The delivery checklist is shown in Delivery Checklist on page 13 To aid in parts identification please see Parts and Materials for Maintenance on page 39 Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 2 Manual Injector Checklist Description Quantity Bio inert Manual injector Handling kit incl valve leak tray connector cable 1 Mounting pole stainless steel p n 5001 3738 1 PEEK SST cap 0 17 mm ID 500 mm long p n 65667 60501 1 Base plate p n G1328 44111 1 Organizer plate p n 5042 8553 1 Catch tube cap p n 5042 8576 1 Syringe 50 uL PTFE FN luer loc
22. sts as they are known for optimum inertness towards biological samples and ensure best compatibility to common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals like iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction does not contain metals at all Table 1 Bio inert Materials Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump Titanium gold platinum iridium G5611A zirconium oxide ruby PTFE PEEK Agilent 1260 Infinity Bio inert High Performance Autosampler Upstream of sample introduction 65667A Titanium gold PTFE PEEK zirconium oxide Downstream of sample introduction PEEK zirconium oxide Agilent 1260 Infinity Bio inert Manual Injector PEEK zirconium oxide G5628A Agilent 1260 Infinity Bio inert Analytical Fraction Collector PEEK zirconium oxide PTFE G5664A Bio inert Manual Injector Table 1 Bio inert Materials Introduction 1 Module Materials Bio inert Flow Cells Standard flow cell bio inert 10 mm 13 ul 120 bar 12 MPa for MWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 p n G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D Max Light Cartridge Cell Bio inert 10 mm V o 1 0 pl p n G5615 60018 and Max Light Cartridge Cell Bio
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