Micro cantilever 取り扱い説明書
Contents
1. Micro cantileverf user s manual Thank you for purchasing OLYMPUS Micro cantilever Please read this manual carefully before use lt Explanation of the each part of the products gt Cantilever chips in a plastic case are included in the envelope with manual and spec sheet Manual and Specification sheet Plastic pack translucent blue Label Inside gt Plastic case Envelope for transportation Cantilever chips are in the case C antilever chips are contained in the plastic case Case for transportation plastic Cover Bottom Cover Label side Arrow mark Upside Hold position Cantilever strips Case closed Case open Cantilevers are tip side up Down side as viewed in the case Cantilever strip and chips Case label Cantilever strip 35chips 1 extra a aa p Products name Tiero C leve OMCL TR400PSA Lt Inspection it No stamp OLYMPUS CORPORANGN Cantilever chip ad Each chip has two cantilevers on each end Lot Number ae OLYMPUS Please obey the following to the OLYMPUS micro cantilevers Use protective eye glasses when handling to avoid damage to the eyes from breakage of the cantilever Use protective gloves to avoid getting hurt by a shard of cantilever chip when breaking and cutting cantilever chips from cantilever strips Caution Please handle our cantilevers carefully because they are fr2. agile AN Caution Do not drop or shake the cantilever case Even when the cantilever strips are contained in the cantilever case the cantilever strips may move in the case and the cantilever may break if the case is handled roughly or jarred Caution Be sure to store the cantilevers at room temperature and moderate humidity Caution When discarding please obey the laws and regulations in your country and or your company These cantilevers are made from silicon nitride gold chromium and pyrex glass 2e Special feature of OLYMPUS Micro cantilever Contact mode Cantilever This type of micro cantilever is suitable for contact mode AFM measurement The small spring constant of the silicon nitride cantilevers enables you to set the tip loading force small to probe the sample surface gently AC mode operation in water This type of micro cantilever is also used for dynamic mode AFM measurement in water For this purpose 100 um long cantilever in OMCL TR400PSA series is recommended Variety of cantilevers with different mechanical properties A suitable cantilever can be chosen from the different types of the cantilevers on a chip Each triangular cantilever type chip TR type has two different cantilevers on each end of the chip Each rectangular cantilever type chip RC type has four different cantilevers on the chip Sharpened pyramidal tip The tip shape about 0 3 micron down from the t
3. ever strip in the case for transportation please pay attention to the following Please avoid storing plural cantilever strips with different lot number in a case Groove Please pay attention to the position I i and the direction of the grooves l where cantilever strips should be A a ere Z placed on Be carefully to lay the a TWS cantilever strips parallel to in the Correct grooves of the bottom cover see right If you place the cantilever on the LONN ANANA TTT inn on Correct bottom case incorrectly cantilevers and cantilever strips may break when you close the case Hold position L aying cantilever in a groove of the bottom cover 8e Correct TCT 2 After you set the cantilever strips correctly in the grooves close the cover and put a piece of the Scotch drafting tape at the lip of the case to fix it 3 Once open the cantilever case level the case with the ground even the case closed illust left Avoid tilting the case with large angle while handling or cantilevers may break by scraping with the stopper pins on the cover if the cantilever strips slide in the case Incorrect Pg Avoid tilting the case with large Stopper pins Cantilever sop The case is always kept even angle while handling with ground 4 Store the cantilevers in a desiccate box for avoiding dust high temperature and humid even the case is closed Storing the cantileve
4. fully open the lid 1 Disconnect the cantilever chip as follows BE 7 A T NRRESI 3 Pull the cantilever strip straight Use protective eyewear when handling to avoid E Lip gt PA y ae up with the tweezers damage to the eyes from breakage of the cantilever chips Warning Pay attention not to touch the A N cantilevers to the case and the AN arn n 2 other strips Use protective gloves to avoid getting hurt by a shard of cantilever chip when breaking and cutting cantilever chips from cantilever strips The way to open the case and to put out the cantilever strip from the case 5e 6e 2 Turn the microscope slide glass sideway for disconnecting a chip see below Then cantilevers on a chip end face to you see right below Cantilever chip Cantilever TET TT Microscope slide glass Cantilever strip ly Lye gw r Cantilever Connecting part Groove 3 Hold the center of the cantilever chip with the tweezers to cut a chip at the end of the cantilever strip Place a scalpel or razor blade at the connecting part between the cantilever chips and scribe a chip off gently Razor blade Tweezers Connecting part Groove Put the scalpel to the edge of connecting part push the scalpel and give pressure to the edge with it like in the drawing above The side face of the scalpel should be kept parallel to the side wall of the groove for making this procedu
5. he case and take out the cantilever strip from the case 1 It is recommended that the cantilever case be opened in a clean environment like a clean bench in order to avoid the cantilever being contaminated Handing under an ionizer is recommended 2 Avoid wearing clothes like woolen sweaters fleece etc that Case give off the static electricity when handling the cantilever Arrow mark cases and chips Use of an anti electrostatic mat and wrist indicate opening position band is preferable 3 In opening the case put the plastic case label side up on a lt Lip desk and turn the case so the arrow mark comes to your natant EN side m LK 7 Tape 4 Peel off the tape to open the case Don t throw away the tape That will be used again to fix the closed case after taking out the cantilever strip from Hold position Bottom Cover Cantilever case Top view the case Be sure to grasp the bottom cover in taking the cantilever case or the case may be opened and the cantilever strips may slip off and break 5 After peeling off the tape from the case be sure to grasp the bottom cover like in the drawing left below Don t pick up the case by grabbing the cover Correct Incorrect If you pick up the cover like in the drawing right the case may open and the cantilever strip may slip off and break Cover Case LI Bottom cover Case ra Pal Slip off Case Cantile
6. ommended because the adhesiveness of the tape is appropriate The use of both side adhesive tapes that is usual in the office is not recommendable because most of them is too sticky Case for storage p Plastic case for 3 5 inch floppy disk _ 7 Nn ag ZA X Tape 2 Tape 1 Tape 3 adhesive side up Cantilever chip The way to store the cantilever chips in the case 3 Be sure to give a memo of the product name and lot number on the case for storage 9 Information Please contact following if you have any question on this user s manual OLYMPUS CORPORATION Microtechnology R amp D Division 2 3 Kuboyama cho Hachioji shi Tokyo 192 8512 Japan email probe olympus co jp Please access to the web page of OLYMPUS micro cantilevers http www olympus co Jp probe Ver 5 0 Aug 6 2009 OLYMPUS CORPORATION 10e
7. op is oxide sharpened so that the tip angle parallel to the cantilever axis is smaller than 70 deg and that the tip radius is smaller than 20 nm The tip material silicon nitride is less prone to wear and tear than a single crystal silicon so that the tips ensure more stable measurements Reflex gold coating Gold is coated on the back side of the cantilevers for optical detection Dual side gold coating types are also available OMCL TR400PB OMCL TR800PB and OMCL RC800PB These make functionalizing the cantilever easier e g using thiol chemistry See the specification sheet of OLYMPUS Micro cantilevers at the last page of this manual Table of contents 0 ioe o Spaifcation OSC COS page E 49 fs ie gt z fe n n Be 1 Please prepare the followings before using OLYMPUS cantilevers 2 To gain a better understanding of how cantilevers and chips are connected cantilevers should be inspected under the microscope 1 Work environment Clean bench Use of an electrical charge neutralizer of ionizer is recommended 2 For hazard avoidance Protective eye glasses Thin gloves 3 For cantilever treatment and disconnecting procedure Microscope slide glass Tweezers Scalpel or razor blade Scotch drafting tape Use of anti electrostatic discharge mat and a wrist band is recommended 4 For cantilever storage Dry box Case for storage 5 For inspection Stereo microscope Open t
8. r strips in the case for storage AN Caution Be sure to store the cantilevers at room temperature and moderate humidity 1 Prepare a case for storing the remaining cantilever strip Plastic case for 3 5 inch floppy disk etc 2 Keep the cantilever strip in the case for storage as it is taped to the microscope slide glass Case for storage Plastic case for 3 5 inch floppy disk E e s Microscope slide glass aa wa RER p ue Siem A p P a SAN ee ee ON fs TRASSSTSEASSSSSA MN sy AU p T 4 w i w sy ee e is Bi al wo heo A Rest of cantilever strip y The way to store the rest cantilever strip in the case 3 Attach the microscope slide glass on the case with a Scotch drafting tape 4 Be sure to give a memo of the product name and lot number on the case for storage Je e the disconnected cantilever chips in the case for stora 1 Store the cantilever chips in another case for storage If you have a GEL PAK container those chips are easily stored in it 2 If you don t have a GEL PAK container there is a way to arrange a substitute case for chips storage by yourself Please refer to the way in the drawing below Like in the drawing below settle the tape 3 adhesive side up on the case with the tape 1 and 2 Using a tape in week adhesiveness as the tape 3 will give you a good result For instance Scotch drafting tape or the adhesive area from a Post it note is rec
9. re easy Connecting part Scalpel or razor blade pe a oe eS Groove i A H jz an tilever chip ee A A fail Sf 4 H ne L j lt 3 lt l E x i j Connecting part f ff De LW L Groove La l Img 5 myer a Y ae a Putting the scalpel parallel to connecting It may be better to press your forefinger on the part is recommended scalpel above to apply vertical pressure to the connection Te Attaching the cantilever chip to your instrument 1 After reading the instruction manual of your instrument again grasp the side of the cantilever chip with the tweezers and attach the cantilever chip to your scanning probe microscope Cantilever chip substrate k Tape Tweezers Es Returning the cantilever strips into the plastic case Caution Lay the cantilever strip correctly in the grooves of the bottom cover when you return the cantilever strips into the plastic case for transportation again Caution Do not drop or shake the cantilever case Even when the cantilever strips are contained in the cantilever case the cantilever strips may move in the case and the cantilever may break if the case is handled roughly or jarred 1 Once you take out a cantilever strip from the case for transportation storing the cantilever strip in a separate case is recommended The way will be explained in the next head 8 in this manual Although it is possible to store the cantil
10. ver strip 4e Preparation before disconnecting the cantilever chip 6 In the procedure 1 to 3 in the drawing below open the cover and take out the cantilever strip with tweezers e If you find the cantilever strip attached to the back side of the cover stop opening the cover Close AN C xut on the case again and pat the cover with your finger to move the cantilever strip from the cover to the i bottom cover Then try to open the case again Do not use the plastic case for transportation as a work plate in the process for chip disconnection AN Cauti 1 Use a microscope slide glass as a work plate in the process for disconnecting the cantilever chip Please handle our cantilevers carefully because they are fragile 2 Lay the cantilever strip tip side up on the microscope slide glass 3 Then settle the cantilever strip on the microscope slide glass with a piece of Scotch drafting tape below f C ution Microscope slide glass Avoid any contact with the cantilevers when you pull up the cantilever Cantilever strip strip from the case A Caution Cantilever chips should always be placed tip side up Tape 1 in the fig Press the hold position on the bottom cover to keep the stationary 2 in the fig Using your thumb carefully open the lid 3 in the fig Pull the cantilever strip straight up with the tweezers Disconnecting the cantilever chip 2 Using your thumb care
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