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1. n 29 UNITMA Quick Ray Master UATM 272 Operating Manual It defines the speed when the High button Fig 5 3 49 gt is pressed at the speed select of the motion controller It does not give any influence on the tack time When the speed is to be modified the touch pad is used to input the number on the text box and make sure to press the Save button to save and apply the value Speed select Position Fig 5 3 49 gt All speed operated in the Auto mode can be defined It does influence the tack time When the speed is to be modified the touch pad is used to input the number on the text box and make sure to press the Save button to save and apply the value i 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 5 Calibration Parameter When the Calibration Param button is clicked the following screen Fig 5 3 50 gt is shown This is to calculate the value of distance from each camera to the tip as well as the pixel resolution of each camera Each of the buttons is arranged as in the calibration sequence and after completing the works make sure to press the Save button to save the values already set Va V SS UNITMA Quick Ray p Tip Pos san er 1pm Tip Pos zm Cam2 Calibration Pos Calibrate Cam2 Register El CC l tip cam Cam Calibration Pos gt Cami el N Register a distance ti
2. Interface with a printer USB i g Printer Interface with an external PC LAN PC Printer USB LAN 29 UNITMA Quick Ray Master UATM 272 Operating Manual 4 3 Connecting to main power 4 3 1 Connecting to main power supply constant and stable AC power source supply to the instrument must be ensured at all times Failure to comply with the above will cause severe damage to the instrument Always use a grounded power cord Do not use defective power cord 1 Set the AC power switch to the off position 2 Refer to Power cords The line cord provided is matched to the country of origin by UNITMA U K Europe U S A Switzerland Denmark 3 Connect the power cord 3m maximum to the rear of the instrument then to a suitable AC voltage source Ensure that you have the correct line cord See 3 3 Instrument specification 4 3 2 Selecting the voltage of AC power source A Do not change the voltage of AC power without authorization from Unitma Co Ltd e Contact a service engineer of Unitma Co Ltd for changing the voltage of AC power source Before selecting the voltage of AC power of the instrument verify if the voltage of AC power intended to use is same as the voltage of AC power source 1 The instrument is free voltage power supply but only PC power is selectable 115V 230 Voltage 2 If the rear side cover is opened it will be showed the below picture of sel
3. 29 UNITMA Quick Ray Master UATM 272 Operating Manual 15UNITMA Quick Ray OOO Core size v1 2 3 set Lam Move Mark Search v EE od ERN Posy BIBIT Tip Move Tip Down Tip Up Select Area lt Fig 5 3 23 gt Manual page 1 Tip Move Push Fig 5 3 24 Manual page 2 To save the value after selecting the Core Size Each core size is met with the same sized tip Manual page 1 C M ml U U U U U U U U To move camera to focus on the selected donor am vove C Cam Move rr block Manual page 1 p To move Camera to focus on the selected hole of the C Cam Move m recipient block When all processes are over press this button again to confirm the condition of the current work Manual page 2 E the Marker Param set in advance In addition the user can choose the position to be inserted on the recipient blocks by using P To fi h k in th l ROI Regi f Mark Mark Search o find the mark in t g SE O a egion S Search 0 Interest Marks found in size sequence is modified by the value of Pos x and Pos y In addition the user can choose the position to extract the sample tissues by using the mouse Manual page 1 Mark Search To find the median value of the hole registered from ki UNITMA Quick Ray Master UATM 272 Operating Manual the mouse Manual page 2 Tip Move Tip Move If the values of Pos x and Pos y are within
4. Disconnect all external cables before transporting the instrument Never attempt to transport the instrument by yourself Three or more people are required to transport the instrument for transporting without causing injury or damaging the instrument Ensure that your footing is solid and balance the weight of the instrument between your feet Transport the instrument slowly and never move suddenly or twist your body during transportation Keep your back straight and transport with your legs not on your back If you must bend down to transport the instrument bend at the knees not at the waist to reduce the strain on your back muscles The weight of the instrument is about 148 kg The instrument should not be moved frequently Before you install the instrument ensure that your site is properly prepared so you can avoid having to move the instrument later to accommodate power sources and network connections To transport the instrument safely perform the following steps 1 Since the weight of the instrument is over 148 kg we recommend four adult people to safely move the instrument 2 Minimum four people working together should hold each corner of the instrument 3 Each person holding one corner with both hands should move in coordination to move the instrument 4 Be careful when moving the instrument to avoid any physical damage Q 35 Cys Fig 4 1 1 gt 29 UNITMA Quick Ray Master UATM
5. Exterior amp Interior e Clean as the instruction 5 1 Cleaning the instrument e Check for readability or missing labels e Check the cord and molded connectors for damage Check for presence of all four legs Check legs for physical damages or deteriorations Power cord receptacle Make sure receptacle is free of foreign matter Check for presence of all three connector pins and make sure they are straight Run Demo mode e Turn on the instrument and run Demo mode See Error message 85 26 UNITMA Quick Ray Master UATM 272 Operating Manual 6 4 2 Replacing the fuse Only use a fuse of the same specification For the required value please refer to the fuse specification in this manual e Unplug the AC power cord Removing or touching the fuse with the AC power cord plugged will expose you to hazardous voltages and cause death 1 Locate the fuse holder module It is located as part of the power cord assembly on the back of the instrument 1 Fuse panel notch 2 Fuse holder 3 Fuse holder module 2 There is a notch at the bottom of the fuse panel where you can insert a flat head screw driver or some other flat object Use leverage to open the fuse panel as it opens upward from the bottom 3 Pull out the fuse holder The fuse holder has a fuse 4 Remove the old fuse by gently pulling it out 5 Insert the new fuse into the fuse holder 6 Slide
6. SS UNITMA Quick Ray lt Fig 5 3 55 gt The orange colored rectangle is the limit sensor and the yellow on is the Limit sensor and the green is the origin sensor When this square turns Origin to red it means that the sensor has the signal As Done the control with the screen controls directly the Lr 5 TI 03 7 Sync motor the button has to be pressed with the location of the tip in mind Fig 5 3 56 de UNITMA Quick Ray Master UATM 272 Operating Manual SS UNITMA Quick Ray Output 00000000 90000000 Input 00000000 INPUT OUTPUT Alam H Axis Reset H V Axis Alam Y Axis Reset 2 Axis Alam 2 Axis SON H Axis Door SON Y Axis Door 2 SON Z AKIS lt Fig 5 3 57 gt While there is no problem with the program five input signals are on as above If there is no signal for 0 1 and 2 it means that the Output 00000000 00000000 input 00000009 motor control has problem and if there is no signal for 4 and 5 it means the front door is open When the signal of 1 or 2 is died out it means the applicable 0 for X Y axis and 1 for Z axis output signal is to be provided to reset the motor alarm When reset is made output is to be released again Output 3 4 and 5 is lt Fig 5 3 58 gt to off motor servo therefore the user is not permitted for the access Ge UNITMA Quick Ray Master UATM 272 Oper
7. block EH move now BE 6 5 col 1 Donor block Donor BEER Press the Move button as in the Manual mode to move XX move to the location of the desired donor block to the location of the desired recipient block hole Motion Speed select controller Se Low lt Fig 5 3 25 gt This part lt Fig 5 3 25 gt is referred to as the motion controller Speed select is to apply the speed set at the Set mode Position displays the position of each axis The plunger tip controls the needle ATC may modify the rotation position of the tip The above Red arrow button may control the X and Y axis and the Blue arrow button located in the middle of the X and Y axis controls the Z axis for Cam 1 Cam 2 and Tip Stop button is to suspend all motions not intended i UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 2 5 Main Menu Press the Main Menu button to move to the initial screen of the program lt Fig 5 3 28 gt 3 UNITMA Creating Human oriented UNITMA technique Imm 50 cores E 3mm 30 cores Quick Ray lt Fig 5 3 28 gt i 9 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 SET When the SET button is clicked the screen is changed as in the following lt Fig 5 3 27 gt and all parameters of the instrument can be set SS UNITMA Donor Block Param we 7 Y 39 fflarker Param
8. ecipient Hole Param D Ki lt Fig 5 3 27 gt The user can input the ID with password for all the limited access At the time of delivering this instrument to a customer Administrator is an ID set with P W of A P W may be changed by the touch key board as shown in lt Fig 5 3 28 gt and lt Fig 5 3 29 gt After inputting P W and pressing the OK button the above screen lt Fig 5 3 29 gt will appear id 29 UNITMA Quick Ray Master UATM 272 Operating Manual gt UNITMA e UNITMA r e ha ve q a O Fu MENNEEENI Cancel d ke lt Fig 5 3 28 gt pe UNITMA Quick Ray Master UATM 272 Operating Manual 9 UNITMA Creating Human oriented UNITMA technique D vg OK Cancel lt Fig 5 3 29 gt 3 UNITMA Creating Human oriented UNITMA technique new uA RENEW PY OK e Jui m A Quick Ray lt Fig 5 3 31 gt UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 1 Donor Block Parameter When the Donor Block Param Button is clicked following screen lt Fig 5 3 30 gt appears This may define the location of each donor block 3 UNITMA Donor Info Himm mm set lt Fig 5 3 30 gt When the button is clicked following messenger box is displayed lt Fig 5 3 31 gt When the OK button is pressed the camera moves AN Move to Donor 1 block position to the position of donor blo
9. 3 gt SS UNITMA Quick Ray Q Column i 2 3 456 gt E lt g y moy 99000000 09000000 90000000 99000000 29000000 09000000 Home Punch cnt BN Sample cot BEN itiali A j x Initialize 00 Set Tray Select Donor Mark Color 3 pic oun Black fed Select ecipient Block lom hecipA Recipe e c B Fig 5 3 3 gt 29 3 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 2 1 Function of each button Description of function To expand the suspension image to be expanded up to 5 0 times by increase in 0 5 at a time The 1 0 time is the current window screen based on 0 5 time of the actual camera resolution To reduce the suspension image to be reduced to 1 0 time by decrease in 0 5 at a time To capture the donor block images with the camera 1 To capture the recipient block images with the camera 2 To convert a suspended image to a live one To acquire a Suspended image from a live one To activate the menu with the button at the right side of the screen for loading a file initializing and starting the process and others To implement each procedure step by step manually in case of failures in recognizing the marks on the donor block and the hole position of the recipient blocks during the automatic execution Refer to section 5 3 2 2 3 In case of failure in recognizing the mark of donor block during the automatic executi
10. Move gt defined under 5 3 2 3 2 Recipient Block Param This is the additional function to replace the tip in safe ways When one of the buttons is Imm mm imm H ell E clicked the tip of selected size is to be located in the front For example if the 1mm button is clicked the 1mm tip is located in the front part of the user Ready position is the place when the tip is replaced and Search position is the value for Z axis when moved to the camera W D Working Distance In addition Calibration position is the Z axis value when the calibration motion is being undertaken The location can be modified by using the Touch pad and make sure to press the Set button to save to the modified location When making the motion related to the tissue insertion the Z axis height can be set The location can be modified by using the Touch pad and make sure to press the Set button to save the modified location Safety Zone is designated not to make the X and Y rotation at the area below certain height to prevent the damage to the tip Sampling displays the location of the upper part of the palette with the tissue raised when the tissue is collected from the Donor block Set Try thrust has to be set as close as possible to the hole with the height to insert the tissue onto the Recipient block s is used to adjust the height for each tip This is to set the location of the needle when inserting the tis
11. UP THE INSTRUMENTT 18 4 1 Installation site requirements 18 ET uuu u saan 20 4 3 Connecting to main power supply 22 5 GENERAL PROCEDURE OF TISSUE MICROARRAY 23 5 1 Building TMA with Quick Ray Master EEN 23 5 2 Preparation for punching and inserting with Quick Ray Master 25 5 3 Operation of the instrument 28 5 3 1 Start and stop of the program 28 5 3 2 KEES 28 e 2 MIB o uu uu EE 28 53290 07 ipo m M M 29 5 3 2 2 1 Function of each DUEEODI E 30 5 3 2 2 2 Program implementation 33 EEE EN NE ad 42 SNE 46 5 3 2 25 MN MN 48 EE WE 49 5 3 2 3 1 Donor Block Paneler E 52 5 3 2 3 2 Recipient Block Parameter 54 3 26 UNITMA Quick Ray Master UATM 272 Operating Manual 59233 Tip Body PN Le 62 OPE 0 10 TE EE EN EE EEEE 65 5 3 2 3 5 Calibration PalaMMe el gege 68 53 23 06 Motion amp IO Property eege eege 72 53 251 Markert PAINE 74 5 3 2 3 8 Recipient
12. can be modified as the user wishes by using the slider bar under the Search button or the and buttons At this time the reduced image is generated on the gray box under the Donor Info and it helps to generate more accurate threshold value with the image It is recommended that the suitable threshold value has to be set before starting the process Otherwise it may cause improper reading of the mark IS UNITMA Quick Ray Search Select Donor Back Color White ib Black ib fed ne E ger Recipient Search Setting lt Fig 5 3 61 gt s 29 UNITMA Quick Ray Master UATM 272 Operating Manual Register 2 a This is to define the size of the Recipient block hole egiter and it shows the following screen as Fig 5 3 62 gt The Set button of the Mark Size is used to define the size of the mark and the territory of the mark to find through ROI can be set too In the event of 1mm the Recipient block hole has so many cases of large and small dents that it would be better to register a little smaller than the original size In addition as the Marker Param does not include the function of selecting the Core Size the user need to modify the Core Size by loading Excel file from the Auto Mode and define hole sizes for each applicable size Mark size e T S UNITMA Quick Ray Image Fite Load leen Reg Model Bir
13. the ROM area the tip can be moved to the position of value area When the position value is deviated from the ROU area the error message shows that the tip cannot reach the area This is to prevent damage of the tip which may occur in the event that the mistaken location is read In this case press the Cam Move button again then it will search the mark again Manual page 1 If the values of Pos x and Pos y are within the 100 100 1180 924 area the tip moves to the position value area When the position value is outside of this area the error message will appear that the tip can not reach the area This is to prevent the damage of the tip that may occur in the event that the mistaken location is read In this case press the Cam Move button again to make the mark search Manual page 2 Tip Down Tip Down The tip is coming down to the cassette surface to extract the sample tissues from the donor block At this time the user should be careful about checking the tip position to prevent the damage to the tip before pressing the button Manual page 1 Tip Move The tip is moved to the surface of the selected hole on the recipient blocks At this time the user should be careful about checking the tip position to prevent the damage to the tip before pressing the button Manual page 2 Push Push To insert the extracted tissue into the recipient block hole Manual page 2 Tip Up Tip Up The tip positioned on the cassette surface
14. to 120 VAC 200 to 240 VAC Selectable by service personnel 3A 0 5A 0 3kw 50 60Hz 148 kg 953 x 703 x 610 mm 10 C to 35 C 5 C to 55 C Max 80 non condensing PC type Industrial chassis CPU Intel Dual Core 2 2GHz RAM 1G HDD 160GB Windows XP PRO MS Excel LCD monitor 15 1 024 x 768 Touch screen 1 280 x 1 024 2 592 x 1 944 pixels 2 sets 2 2um x 2 2um Color 30um Donor block Sum Recipient block LED type 2 sets UNITMA Recipient block consumable 1 2 and 3mm 3 types 10 Donor blocks and 2 Recipient blocks 15 18sec Pick amp Place Indoor use only II Illa or IIIb Up to 10 26 UNITMA Quick Ray Master UATM 272 Operating Manual 3 4 Consumables Recipient block The patented recipient block is made of a special material that melts when heated at 60 C for 30 minutes The recipient block has pre made evenly spaced round orifices wells arranged in a square matrix array Three different recipient blocks are available CEEKEEEKEEK 3333333338 5559595955555 5999999999 8838233383938 83333333835 lt Fig 3 4 1 gt 10 x 12 wells of 1mm in diameter 6 x 10 wells of 2mm in diameter 5 x 6 wells of 3mm in diameter Weights 3 5g Use only the pre made recipient blocks UB06 supplied by Unitma Co Ltd ue 26 UNITMA Quick Ray Master UATM 272 Operating Manual 4 SET UP THE INSTRUMENT 4 1 Installation site requirements e Transporting the instrument A
15. 0 5mm then it moves to X axis direction Y axis direction and Y axis direction and the applicable pixel distance is calculated for the central point of each mark to formulate the resolution by the proportion And the location is arranged to have the mark center to come into the central part And the distance between the tip and camera 1 will be calculated automatically SUNITMA Quick Ray Help ext Move Tip Pos Register Tip Pos Tip Pos NENNEN masus Move Cam Calibration Pos Calibrate Came Cal distance tip came Move Cami Calibration Pos Cal distance tip cam Speed select lt Fig 5 3 54 gt Cal distance tip If this button is pressed it calculates the distance from the location of the CAM 1 tip calculated earlier and the central location of the camera 1 This is the most important button After completing every process as in the above sequence make sure to save new values to be applied to the program When the Back button is used new values are saved automatically but when the screen conversion is made by clicking the main menu in the sub menu they are not saved at all 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 6 Motion amp IO Property The Motion amp IO Property button is clicked to have the following screen as lt Fig 5 3 55 gt This is to modify the set value of each motor axis and some sensor condition may be monitored through I O
16. 2 1 gt for more details L lt Fig 5 3 12 gt UNITMA Quick Ray Master UATM 272 Operating Manual The above figure lt Fig 5 3 12 gt shows the condition loaded all blocks Start To implement the process as programmed 1 When using the new recipient block it has the function to mark the position of the first hole that uses 1mm tip to easily confirm the recipient block direction Following figure lt Fig 5 3 13 gt shows the location with the mark The indicated location is set automatically in meeting the size of the hole OO ses O O Marking lt Fig 5 3 13 gt Following figure lt Fig 5 3 14 gt shows the direction on the recipient block lt Fig 5 3 14 gt 2 In order to extract the marked tissue from the donor block the instrument recognize the mark on the donor block and pick the sample tissues and deliver the extracted tissues into the correspondent hole of the recipient block automatically Following figure lt Fig 5 3 15 gt shows the condition Se UNITMA Quick Ray Master UATM 272 Operating Manual when the tip is descended during the process of extracting the tissue lt Fig 5 3 15 gt Following figure lt Fig 5 3 16 gt shows the descending condition of the tip and needle during the insertion process on the recipient block lt Fig 5 3 16 gt 3 After completing the process The ID of donor sample tissues extracted earlier is prepared on the applic
17. 272 Operating Manual e Floor loading requirement Ensure that the floor under the instrument is capable of supporting all other installed instrument The instrument is about 148kg Do not put any objects on the instrument or accessories e Positioning the instrument 1 The left side of the instrument must remain unobstructed to ensure adequate airflow and prevent overheating inside the instrument Refer to next instruction Ventilation requirement 2 The interface port in the right side of the instrument is for connecting the cables for interfacing external devices Ensure that connecting cable is not obstructed Power inlet Interface port Fig 4 1 2 gt 3 The power cord in the right side of the instrument is for connecting the power cable to AC power source Ensure that connecting cable is not obstructed 4 Allow the following clearances for normal system maintenance At the top of the instrument At least 2ft 60 96cm In the front of the instrument At least 4ft 121 92cm e Ventilation requirement The air intake and exhaust areas must be free from obstructions Unrestricted air flow is required for proper cooling Ensure that air flow is not obstructed Fig 4 1 3 gt When installing or using the instrument provide at least 4 100mm clearance left from the instrument for proper cooling e Relative humidity non condensing Operating 20 to 60 Storage 20 to 60 P 99 UNITMA Quick Ray M
18. As in the Manual mode the Move button is used to move camera to the location of the desired Donor block and the location of the Recipient block hole We 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 8 Recipient Hole Parameter This is the screen shown as lt Fig 5 3 66 gt when the Recipient Hole Param button is pressed This is to find the mark defined for the location of the donor to be collected and the central point of the Recipient block hole Quick Ray Column i 23 4 5 6 gt E Dd D m x select Tode Columntl 2 3 4 5 6 gt H LJ H lt 9 9 moy 0 0 C C 0 0 C 0 0 0 C 0 0 C 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CU 0 0 0 0 0 0 0 7 0 0 0 0 0 0C 0C OC 2 0 0 0 0 0 0 0C 0 0 0 0C C 0 0 C C 0 0 0 0 0 0 0 0 0 0 0C C 0 0 0C C 2 0 0 C 0 0 0 0C Select Area x lt Fig 5 3 66 gt Description of function The combo box is used to select the core size Select Area SS From the Recipient Select rea block holes hole to CG Guna epes se and hole rotte us may be selected Select Area according to user s i UNITMA Quick Ray Master
19. Hole Parameter 79 ED EEE EE EE EEE er EEE E 81 6 CLEANING amp MAINTENANCE 82 6 1 Cleaning Hagen 82 5 2 Replacing consumable uuu ULU Sa asasssaabusaqukawkaswanaakasqsspaqanusaqawkkuqaqaass ayasa 83 6 3 Replacing components 84 ba MAMENANG E 85 05 REPA EE 86 6 6 Disposal AV 86 7 TROUBLE SHOOTING 88 8 WARRANTY amp SERVICGE 90 4 26 UNITMA Quick Ray Master UATM 272 Operating Manual 1 IMPORTANT NOTES 1 1 Explanation of Symbols used WARNING WARNING indicates an injury hazard not immediately accessible as you read this symbol It calls attention to an operating procedure practice or the like that if no correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met CAUTION CAUTION indicates a hazard not immediately accessible as you read this symbol It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met Protective Ground or Earth Terminal Used to indicat
20. NITMA technique Imm 120 cores Quick Ray lt Fig 5 3 68 gt As above if the EXIT button is pressed from the main menu or presses the Exit button on the title bar as in the following figure the program is existed SS UNITMA Quick Ray CES At this time be noted that do not press the Exit button when the program motion is not completed in the Auto mode And when the program is in the Pause condition the program cannot be existed Make sure to complete the motion or press the suspend button to exit the program 5 3 2 5 HELP When the Help button is pressed on the title bar at the upper part of the program this file as the manual of UATM can be accessed r SS UNITMA Quick Ray Mao 81 26 UNITMA Quick Ray Master UATM 272 Operating Manual 6 CLEANING amp MAINTENANGE 6 1 Cleaning the instrument For safety unplug the AC power cord from the electrical outlet before cleaning Do not allow liquid or small particles to get into the instrument or accessories when cleaning Cleaning the connectors Do not use when the connecting cables of the instrument and AC power cord are not clean Remove the dirt with a dry cloth or cotton swab When being used under unclean condition the flow of electrical current may be obstructed Cleaning the LCD screen Wipe gently with a soft cloth Be careful to avoid scratching on the LCD screen e Cle
21. Ray ami Came Li dirab Lie Help Exit Position Info Himm YI mml I 333333 lt Fig 5 3 42 gt gt Screen lt Fig 5 3 42 gt shows captured image when the button is clicked 59 26 UNITMA Quick Ray Master UATM 272 Operating Manual Calibration 1mm Mark is made to move to the central part of the CAM 2 screen Tip It is desirable for the Mark to be positioned at the center of the screen but a little skewed position to the northeast direction makes the calibration easy E UNITMA Quick Ray Master UATM 272 Operating Manual The starting location of the Recipient block hole can be designated At this time it would be prudent to select the location of 1mm and this is attributable to the standard for the center of cross line to be the Recipient block mamng H LS UNITMA Quick Ray Recipient 2 erer set Gap RS aa ze Fig 5 3 43 gt As in the Recipient 1 it may designate the starting location of the Recipient block hole At this time it would be prudent to select the location of 1mm and this is attributable to the standard for the center of cross line to be the la block marking B UNITMA Himmal Y Lmm 43131131 psum IE set Gap ws set enunt eg zo speed select step Bok S 2 E Ma Main SENG menu lt Fig 5 3 44 gt This is the hole gap The location inputted by Recipient and Recipient 2 becomes A 1 and ot
22. Recipient block holder bottom and the block holder frame gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the exterior of the instrument 6 2 Replacing consumable Consumables list Three different size Recipient blocks 10 x 12 wells of 1mm in diameter 6 x 10 wells of 2mm in diameter 5x6 wells of 3mm in diameter e Replacing the Recipient block d Keep the tray door of the instrument in a safe distance from your head and face Be careful for your fingers or hand not to be injured by the needle of tip when assembling or disassembling the recipient blocks from the recipient block holder tray Be careful not to compress your fingers when closing the tray door of the instrument 1 Wait until the operation of the instrument is finished and stopped 2 Open the tray door 3 Disassemble the Recipient block holder tray from the jig for the Main plate of the instrument 5 Disassemble the Recipient block from the Recipient block holder 4 Disassemble the Recipient block holder from the Recipient block holder tray 6 Insert the new Recipient block into the Recipient block holder 7 Put the Recipient block holder with the new Recipient block into the block holder tray 29 UNITMA Quick Ray Master UATM 272 Operating Manual 8 Assemble the Recipient block holder tray into the Main plate of the instrument 9 Close the tray door 6 3
23. Replacing components e Replaceable components list 1 Puncher 2 Tip 3 Block holder tray 4 Recipient block holder d Keep the cover door of the instrument at a safe distance from your head and face Be careful with your fingers or hand not to be injured by objects to be replaced Be careful not to compress your fingers when closing the cover door of the instrument e Replacing the puncher 1 Unplug the AC power cord from the electrical outlet 2 Open the front door 3 Disassemble the puncher from the puncher module of the instrument 4 Assemble the new puncher into the puncher module of the instrument 5 Close the front door Make sure the diameter of tip s needle of the new puncher is the same as with the diameter of tip s needle of the disassembled puncher Check that the position of the new puncher in the puncher module is the same as with the position of the disassembled puncher module e Replacing the tip 1 Unplug the AC power cord from the electrical outlet 2 Open the front door 3 Disassemble the puncher from the puncher module of the instrument 5 Replace with new tip and tighten the screw then assemble the new tip into the puncher body 4 Loosen the screw of the tip and disassemble the tip from the puncher body 6 Assemble the new puncher into the puncher module of the instrument T Close the front door di 99 UNITMA Quick Ray Mast
24. UATM 272 Operating Manual It saves the information of Recipient block hole for its being included or excluded Select the area method to use first as in the following screen Fig 5 3 67 gt After selection of inclusion press Save button and the window of the Recipient block condition changes The situation of the horizontal situation is omitted from the screen Column i 2 3 u 5 6 gt Column i 23 456 gt Column i 23 4 5 6 gt lt 29 b moy lt 29 y moy lt 29 y moy N F N d k d SER mus O Te O s O Column I 23 4 5 6 gt Column l 2 3 4 5 6 gt Column 2 3 456 gt L 4 L E JC OC OC OC OC OC YC OC OC E IE EIT 0 0 0 0 l L H JE JE Li gt D m lt 2g HIM lt g by Moy CC 1000000000 CC 100000000 CC 100000000 CC 1000000000 CC 1000000000 CC 1000000000 00000000 CC 00000000 00000000 CC J00000000 CC 1000000000 CC CC 10000000 CC JE OC OC OC OC OC OC CC 1000000000 CC 1000000000 CC 1000000000 JE OC OC OC EK EE 1000000000 CC Select Area Select Area Select Area x Select Area Select Area Select Area Guides Seier O pi ede O Gabe C See lt Fig 5 3 67 gt _ 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 4 EXIT For the method to EXIT the program there are two ways S UNITMA Creating Human oriented U
25. User Manual Automated Tissue Microarrayer Quick Ray Master UATM 272 en UNITMA eo www unitma com Quick Ray Master UATM 272 Operating Manual NOTE This manual describes the installation and operation of Quick Ray Master UATM 272 Review this manual to avoid injury and prevent damage to this product or any products connected to it before you operate this instrument To avoid potential hazards use this product only as specified in this manual This documentation contains information and warnings that must be followed by the user to ensure safe operation and to maintain the product in a safe condition Customer shall be responsible for paying all shipping charges duties taxies and any other charges for any failure or damage or injury caused by improper use or improper or inadequate maintenance and care Unitma Co Ltd shall not be obligated to furnish service under this case a to repair damage resulting from attempts by personnel other than Unitma Co Ltd representatives to install repair or service the product b to repair damage resulting from improper use or connection to incompatible equipment c to repair any damage or malfunction caused by the use of non Unitma Co Ltd supplies or d to service a product that has been modified or integrated with other products when the effect of such modification or integration increases the time or difficulty of servicing the product CopyrightO Unitma Co Ltd All r
26. a amp Hista Blab amp Track Rosuts Model Ne 0 100 1 ADD Model Size mar TS E wl were ale ca 5 Ro gp Wide mu High lt Fig 5 3 62 gt lt ROI gt nonsens Hl Reg Model BinafHisto Blob amp Track Results Model No p tn 1 ano bodel Size wak er r i Cal bal Rew H weie 271 High 252 lt Fig 5 3 63 gt i 29 UNITMA Quick Ray Master UATM 272 Operating Manual tetting Properi ke Image Fite Load noname Reg Modal Beu amp Histo Blab amp Track Rasuna Thueshaldifi 255 an Sel lt Fig 5 3 64 gt On the Bina amp Histo tap the present image histogram is shown lt Fig 5 3 64 gt and through this the threshold value can be set for easy finding of the Recipient block hole On the Blob amp Track tap the Execute button is used to find the mark in advance In the result the information of mark found is included The basic value is set in advance but if the recognition rate of the hole is declined take a look at it again Search M When the Search button is pressed the location of the Recipient block hole can be found as in the following figure Fig 5 3 65 gt Quick Ray Select Donor Mark Color White ib Black e hed A0 Search RE ms Recipient Search Setting amasisa TJ Sr or TT cn lt Fig 5 3 65 gt 99 UNITMA Quick Ray Master UATM 272 Operating Manual Move
27. able location of the Excel sheet made with the same shape as the recipient block for completing one step Block During the process implementation in the event that the donor block or replacement recipient block is used up the messenger window shows to replace the blocks as shown in the below lt Fig 5 3 17 gt At this time the main plate will move to the tray door for easy replacement of the blocks After replacing the donor block or recipient block click the Complete button on the alarm messenger window and click the Continue button from the screen the SS 29 UNITMA Quick Ray Master UATM 272 Operating Manual process would be implemented continuously EES Columnii 23 u 5 amp gt Select Recipient Block Ou Wheciph heciph Copy ES Select Recipient Block QAI Ghedph Gong Groe E lt s gt ie Ta SG it ats k au EEN A Sege menu e enge lt Fig 5 3 17 gt UNITMA Quick Ray Master UATM 272 Operating Manual Finished NA Quick Ray Set Tray ES a Sample coc RES tialize D Set Tray donor Mark Color DL Select Recipient Block OM hedp Shecips d Copy ENN fiumi Be lt Fig 5 3 18 gt And then when all extraction and insertion processes are completed it has the alarm messenger window lt Fig 5 3 18 gt to indicate the process completion lt Fig 5 3 19 g
28. al number and or the warranty seal removed or made ineligible 7 Scratches or other cosmetic damage to the product that do not affect the operation 8 Normal and customary wear and tear 9 Force Majeure any damage beyond the reasonable control of Unitma such as but not limited to Fire Flood Earthquake act of Terrorism etc 10 Shipping costs for pick up and delivery from your facility 8 3 Contact for technical service Please contact UNITMA CO LTD for technical service UNITMA
29. and subsequently delivering them into recipient blocks 1 The user inserts donor blocks into donor block tray and a recipient block into Recipient block holder tray and puts each block tray into the Main plate Donor blocks should be made from paraffin embedded tissues and recipient block should be just only UBO6 supplied and made by UNITMA Co Ltd 2 The user can see images of donor blocks and recipient blocks on a built in LCD monitor and can use the touch screen to adjust settings and run the UATM 272 User can select and save the specific position for extraction of tissue core on the selected donor block image and the specific hole of corresponding recipient block by clicking the block image and button image of the touch screen 3 When the user clicks the UATM exe button in software program for the TMA procedure UATM 272 executes the TMA block construction process as programmed by the user 4 The user can print the TMA report generated in UATM 272 with an external printer and save the Excel files of the recipient block data into a USB memory stick d The use of the instrument for any other purpose than originally intended may damage the instrument or injure the user and will void all warranties 26 UNITMA Quick Ray Master UATM 272 Operating Manual 2 SAFETY NOTES Read the following safety notes to avoid injury and prevent damage to this product or any products connected to it Misuse of electrical equipment ca
30. aning the exterior surface Wipe gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the exterior of the instrument Clean the ventilation grids on the side of housing panels with a brush or vacuum cleaner to remove dust and dirt e Cleaning the interior surface Open the cover door and wipe gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the interior of the instrument e Cleaning the puncher and tip Keep the front door of the instrument at a safe distance from your head and face Be careful with your fingers and hands not to be injured by the needle of tip or the block holder tray during assembling or disassembling or when cleaning the 1 Unplug the AC power cord from the electrical outlet 2 Open the front door 3 Disassemble the puncher from the puncher module of the instrument 99 UNITMA Quick Ray Master UATM 272 Operating Manual 4 Wipe the puncher body and the tip gently with a damp cloth and then dry the surface Do not use solvents or other chemicals to clean the exterior of the instrument e Cleaning the block holder tray 1 Unplug the AC power cord from the electrical outlet 2 Open the tray door 3 Disassemble the block holder frame from the instrument 4 Disassemble the Donor block and the Recipient block holder from the block holder tray 5 Wipe the Recipient block holder top and the
31. aster UATM 272 Operating Manual e Temperature Operating 10 C to 35 50 F to 95 F Storage 5 C to 40 50 F to 143 F e No direct sunlight e Do not use an extension cord A Do not operate the instrument in rooms where possible accumulation of gases may cause explosion or explosive materials are stored 4 2 Setup 4 2 1 Fitting the puncher into the instrument e Punchers are assembled when you receive the instrument If the Tip needle become damaged or looses sharpness you need to change them as the following instructions The puncher should be put into the correct position in the puncher module of the instrument 1 Turn off the instrument 2 Open the front door 3 Put the puncher into the puncher module of the instrument H re Cem 4 Close the front door lt Fig 4 2 1 gt Puncher Module a 99 UNITMA Quick Ray Master UATM 272 Operating Manual 4 2 2 Interfacing with the external devices Any electrical external devices interfaced with the instrument should be connected to ground e External device list 1 Monitor with a DVI port 2 Mouse with a USB port 3 Keyboard with a USB port 4 USB Memory stick 5 Printer with a USB port 6 PC with a LAN port e Interfacing with the external devices lt Fig 4 2 2 gt Interface with an external monitor DVI Monitor Mouse Pa Keyboard USB Interface with an USB memory stick S USB Memory
32. ating Manual 5 3 2 3 7 Marker Parameter This is to show the screen when the Marker Param button is pressed as lt Fig 5 3 59 gt It finds the mark set for the location of the donor to be collected and the focal point of the Recipient block hole SSUNITMA Quick Ray Select Donor Mark Color Q White Black lt Fig 5 3 59 gt Button Description of function Select Donor Inspector may select the color of the mark displayed with the location of the tissue Mark Color to be collected from the Donor block It displays the territory to find the donor marked by Y Ol the red line shown on the screen This ROI territory may be set again For example if the following territory is set it reads the mark located on the left upper side from the four marks on the screen When the ROI button is pressed the red square is turned into the dotted line to change the value and it is saved if the ROI button is pressed again Ge 29 UNITMA Quick Ray Master UATM 272 Operating Manual gt S UNITMA Quick Ray Select Donor Mark Color White Black fed o JE move Search Recipient Search Setting 1H Fig 5 3 60 gt Select White at the Select Donor Mark Color and 4 press the Search button to find the location of the white point of the mark shown on the tissue as shown in the following figure lt Fig 5 3 61 gt The already set threshold value
33. cipient block Recipient block holder top Recipient block holder body Adjust screw e Main plate picture 1 Main plate 2 Calibration point 3 Donor block tray 4 Recipient block holder tray lt Fig 3 2 4 gt Main plate Recipient block holder lt Fig 3 2 5 gt Recipient block holder e Recipient block holder tray 1 Recipient block holder tray 2 amp 3 Recipient block holder lt Fig 3 2 6 gt Recipient block holder tray e Set up trays 1 Recipient block holder 2 Recipient block 3 Donor block 99 UNITMA Quick Ray Master UATM 272 Operating Manual lt Fig 3 2 8 gt Puncher lt Fig 3 2 7 gt Set up trays e Puncher 1 Puncher body 2 Tip 3 Tip needle 4 Plunger Core ejector 15 26 UNITMA Quick Ray Master UATM 272 Operating Manual 3 3 Instrument specifications Approvals Nominal supply voltages Nominal supply current Nominal frequency Weight Max size W x D x H Operating temperature range Transportation Storage temperature range Relative humidity Operating Control type Operating system Monitor Camera resolution Camera CCD Size Camera spectral sensitivity 1 Pixel resolution Illuminating Recipient block Punch size Tray stage capacity Time per 1 cycle Operating environment condition Over voltage Category Pollution degree Mains voltage fluctuation UL CE ISO Selectable by service personnel 100
34. ck 1 and if the Cancel EG button is pressed no motion will be made Cancel lt Fig 5 3 31 gt ee 29 UNITMA Quick Ray Master UATM 272 Operating Manual SS UNITMA Quick Ray Donor Info Set lt Fig 5 3 32 gt As above lt Fig 5 3 32 gt the location can be set and modified by using the next X and Y values with the unit of mm Each of X and Y values display the location at the original point found In order to change the value click the left text box to have the Key pad NumPad to show lt Fig 5 3 33 gt Position X 36 2135 With the NumPad input the desired location value and press OK 86 2135 button then the NumPad is disappeared and the input number is BET A LE copied on the Text box In order to apply it the Set button on the right 341 241 0 ER has to be pressed Ig Iu cancer lt Fig 5 3 33 gt 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 2 Recipient Block Parameter eee Recipient Block Param 00 When the Recipient Block Param button is clicked the following screen Fig 5 3 34 gt is appeared The user can define the location of each recipient block Himm mm Position Info Fig 5 3 34 UNITMA Quick Ray Master UATM 272 Operating Manual Description of function Standby Pos The location can be designated when replacing the so called Set Tray Donor block or Recipient block hol
35. ck holder tray from the Main plate of the instrument 4 Take out the recipient block holder from recipient block holder tray 5 Take out the recipient block from recipient block holder 6 After taking out the recipient blocks replace the recipient block holder into the Main plate 7 Close the tray door a 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 Operation of the instrument 5 3 1 Start and stop of the program 9 UNITMA Creating Human orien ted UNITMA technique Imm 120 cores Quick Ray lt Fig 5 3 1 gt 5 3 1 1 Start When the UATM exe on the base screen of Window XP is double clicked the Quick Ray UATM 272 program starts to implement as shown in Fig 5 3 1 gt 5 3 1 2 Stop When the EXIT is clicked the program is stopped And then turn off the computer and consequently turn off the instrument by the main power S W 5 3 2 Operation Procedure 5 3 2 1 DEMO This function is to show the simple operation without the execution of the originally intended work When the DEMO is clicked the instrument starts to show all procedures without extracting and inserting the sample tissues for helping the users understanding UNITMA Quick Ray Master UATM 272 Operating Manual 3 UNITMA Creating Human oriented UNITMA technique lt Fig 5 3 2 gt 5 3 2 2 AUTO When the AUTO is clicked the screen is changed as shown in Fig 5 3
36. de for quick and easy use and a semi automatic mode for manual user The instrument has a built in computer run by MS Windows XP OS and an LCD monitor with a touch screen and is also connected to an external monitor USB keyboard and USB mouse Also the quick help function key is available for various tips including instructions on saving image files and reporting the information of recipient blocks The user can also save image files and report files through an external USB memory and an external PC with LAN interface be 99 UNITMA Quick Ray Master UATM 272 Operating Manual 3 2 Instrument components 4 Inner view of the instrument 1 Puncher Module 2 Puncher 1mm 2mm and 3mm Camera 1 Camera 2 Light controller Computer Front door sensor Tray door sensor Oo OO N O QO C Main plate 10 Donor block tray 1 10 oOo OO N O QO A C N lt Fig 3 2 3 gt Right view of the instrument e Front view of the instrument 1 Front Door 3 2 Tray Door 3 Keyboard drawer 4 LCD Monitor Touch screen lt Fig 3 2 2 gt Inner view of the instrument e Right view of the instrument 1 Right side door I O port panel Main power switch PC power switch Monitor connector DVI type USB connector 1 USB connector 2 LAN connector Name plate 10 A C power inlet 13 26 UNITMA Quick Ray Master UATM 272 Operating Manual RR O N Re
37. door of the instrument is opened Close the front door before operating this instrument Do not operate with suspected failures If you suspect that there is damage to the product have it inspected by Unitma Co Ltd Avoid exposed circuitry Do not touch exposed connections and components with the power turned on Do not operate in wet damp conditions Do not operate in a high concentration of explosive gases atmosphere Keep the instrument surfaces clean and dry Do not allow liquid or small particles to get into the instrument or accessories Do not throw or drop the system or accessories or subject the instrument to strong physical shock Unplug the AC power cord from the electrical outlet before cleaning 26 UNITMA Quick Ray Master UATM 272 Operating Manual Using in proper environment Provide proper ventilation Refer to the manual s installation instructions for details on installing the product so it has proper ventilation Do not expose the system or accessories to high temperatures high humidity or direct sunlight Do not expose the instrument or accessories to dust smoke or steam Do not place the instrument on surfaces that are tilted unstable or subject to vibration Do not put heavy objects on the instrument or accessories Do not use the instrument where oxygen gas is used To avoid personal injury Be careful not to compress your fingers when closing
38. e When this button is clicked it moves to the designated location lt Fig 5 3 37 gt The location may be modified by using the touch pad and make sure to press the Set button to save the location modified lt Fig 5 3 37 gt When the tip is replaced the location can be designated When this button is clicked it moves to the designated location The location may be modified by using the touch pad and make sure to press the Set button to save the location modified When calibrating the end part of the Tip would come to the center of the calibration mark area by designating the location to measure the distance between each camera and tip When this button is clicked it moves to the Cal pos 2mm designated location The location may be modified by using the touch pad and make sure to press the Set button to save the location modified 2 29 UNITMA Quick Ray Master UATM 272 Operating Manual lt Fig 5 3 38 gt gt This above picture lt Fig 5 3 38 gt shows the Tip calibration position within the instrument when the Cal pos 1mm button is clicked 99 UNITMA Quick Ray Master UATM 272 Operating Manual When the calibration of Camera 1 is made the location can be designated When the 1mm tip location is set 2 and 3mm approximately 10mm distance from each other is set automatically When this button is clicked in the following figure it moves to the designated location The loca
39. e a circuit common connected to grounded chassis Main Power Connected or ON C Main Power Not Connected or OFF ml Pushed PC POWER on L Pulled PC POWER off BIOHAZARD WARNING BIOHAZARD WARNING indicates an injury or hazard not immediately accessible as you read this symbol Bio hazard Infectious agent A type of micro organism bacteria mold parasite or virus which normally causes or significantly contributes to the cause of increased morbidity or mortality of human beings 26 UNITMA Quick Ray Master UATM 272 Operating Manual 1 2 Name plate The name plate of UATM 272 is at the Model Mame UATM 272 right side of UATM 272 The name plate SAR AG LEGE LE Selectable by service personnel indicates the serial number year of O 100 to 120 Y 3 04 50 60Hz l 1200 to 240 V 1 5A 50 60Hz manufacture the power rating and APPLIANC INLET FUSE F3 1EAL2SBIV SERIAL No DATE OF MANUFACTURING MAUNFACTURED BY UNITMA CO LTD LISTED MADE IN KOREA www unitma com x ean frequency 1 3 Qualification of personnel The Quick Ray Master UATM 272 should be operated by trained Histopathology laboratory personnel All laboratory personnel designated to operate UATM 272 is required to read this entire manual before operating UATM 272 1 4 Intended use of the instrument The Quick Ray Master UATM 272 is an automated Tissue Microarrayer for extracting the tissues from donor blocks
40. ectable switch on the top area of PC computer and it can be adjusted according to the local supply voltage 100V 120V 200V 240V Fig 4 3 1 gt 99 UNITMA Quick Ray Master UATM 272 Operating Manual 5 General procedure of Tissue Microarray 5 1 Building TMA with Quick Ray Master 5 1 1 Place the reference slide and the donor block on microscope stage for marking the position with an oil pen Fig 5 1 1 gt 5 1 2 Punch the tissue specimen from donor blocks and deliver them into recipient blocks by using Quick Ray Master 1 Place donor blocks tray and a recipient block tray on the Main plate in Quick Ray Master Fig 5 1 2 gt 2 Punch the tissue specimen from donor blocks and deliver the extracted tissues into recipient blocks by using Quick Ray Master Fig 5 1 3 3 Disassemble the recipient block from recipient block holder tray in Quick Ray Master 5 1 3 Put the recipient block into an embedding mold with the cutting section faced down and incubate it in an Oven at 60 C for 30minutes Fig 5 1 4 gt e 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 1 4 Embedding Process 1 Once the recipient block is completely transparent 2 Place the plastic cassette on top of this recipient block 3 Pour hot embedding paraffin on the plastic cassette lt Fig 5 1 5 gt 5 1 5 Solidify the block in a cold plate 5 1 6 Cut the block with the m
41. elect the location of the OAI GhecipA ecip Copy recipient block to be used To move the main plate to the front door for setting 4 Qt Set Tray Following figure lt Fig 5 3 9 gt shows the Main plate moved to the tray door donor blocks and recipient blocks on it i UNITMA Quick Ray Master UATM 272 Operating Manual lt Fig 5 3 9 gt When the main plate is positioned at the tray door the user has to take out the donor block tray and the recipient block tray from the Main plate Then put the pre marked donor blocks into the donor block tray and put the prepared recipient block holder into the recipient block tray And then fit the donor block tray and the recipient block holder tray into the main plate The user has to mark the desired area to be extracted from the donor blocks at least 2 hours before putting the donor blocks into the donor block tray If the marking ink is not dried up completely it may cause in non insertion of the extracted sample tissues ii UNITMA Quick Ray Master UATM 272 Operating Manual lt Fig 5 3 10 gt The above figure lt Fig 5 3 10 gt shows the white marker to display the desired position to be extracted lt Fig 5 3 11 gt As shown on the figure lt Fig 5 3 11 gt when the user put the recipient block into the recipient block holder the user has to be careful about the direction to meet the left odd on the holder body see the lt Fig 5
42. er UATM 272 Operating Manual O e Make sure the diameter of tip s needle of the new tip is the same as with the diameter of tip s needle of the disassembled puncher body Check that the position of the new tip in the puncher body is the same as with the position of the disassembled puncher body e Replacing block holder tray 1 Wait until the operation of the instrument is finished and stopped 2 Click on Set Tray button 3 Open the front door 4 Disassemble the Recipient block holder tray from the jig for the Main plate of the instrument 5 Assemble the new Recipient block holder tray into the jig for the Main plate of the instrument 6 Close the front door e Replacing Recipient block holder 1 Wait until the operation of the instrument is finished and stopped 2 Click on Set Tray button 3 Open the front door 4 Disassemble the Recipient block holder tray from the jig for the Main plate of the instrument 5 Disassemble the Recipient block holder from the Recipient block holder tray 6 Put the Recipient block holder with a new Recipient block into the Recipient block holder tray 7 Assemble the Recipient block holder tray into the jig for the Main plate of the instrument 8 Close the front door 6 4 Maintenance 6 4 1 Checking the instrument UNITMA recommends that the instrument be checked by the user at least once every six months or whenever you move the location of the instrument Check
43. he warranty period provided it is returned in accordance with the term of the warranty Replaced parts are warranted for the balance of the original applicable warranty period All replaced parts of the products shall become property of UNITMA 2 This limited warranty is extended by UNITMA to the original end user purchaser and is not assignable or transferable to any other third party 3 UNITMA can not be responsible in any way for any additional equipment not furnished by UNITMA which has been attached to or used in connection with the product 4 Customer must provide proof of purchase bearing the date of purchase and the product s serial number in order to receive warranty service Warranty service will be provided by UNITMA through one of its authorized warranty service centers 8 2 The Warranty does not cover 1 Defects or damage resulting from the use of the product other than its normal customary use 2 Defect or damage from misusage such as breakage spillage of liquid or substances that can cause circuit shortcut of the electronic boards and battery leakage etc 3 Defect or damage from improper testing operation installation alternation modification or adjustment of the instrument 4 Breakage or damage unless caused directly by the defects in material and workmanship 5 A product with unauthorized product modifications dissembles and or repairs which adversely affect the performance of the product 6 A product with seri
44. hen this error message appears please initialize at the Auto mode menu screen Not Execute Excel To write the report of the Recipient block you need Excel program installed and functional on the instrument s PC Please check for software installation instructions S 99 UNITMA Quick Ray Master UATM 272 Operating Manual Not measured the height block Check Recipient block amp tool Front door is opened Please close the door 89 When Run button has been pressed without measuring the heights of each block this error message will be displayed From Auto Mode screen touch Measure button for the instrument s height gauge measures the height of each block and can run the program To run the program you need to visually check whether both the size of the tool s tip and Recipient block matches You need to confirm that it is the correct size to run the program In order to run the program the cover door must be closed Please check the door is closed and run the program 26 UNITMA Quick Ray Master UATM 272 Operating Manual 8 WARRANTY amp SERVICE UNITMA CO LTD UNITMA has limited warranty for the Quick Ray Master against defects in material and workmanship under normal use and service for period of 1 year from the date of purchase 8 1 Terms amp Conditions 1 UNITMA will at no charge either repair the Quick Ray Master with new parts or replace it with a new unit during t
45. her holes use this gap value to make automatic calculation Input the vertical and horizontal number of the holes for the Recipient block 61 26 UNITMA Quick Ray Master UATM 272 Operating Manual 1mm is for 12 x 10 and 2mm for 10 x 6 and 3mm for 6 x 5 This value shall not be changed 26 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 3 Tip Body Parameter The Tip Body Param button is clicked to show the following screen lt Fig 5 3 45 gt The users can be define the height and location of the applicable tip from each process Tip Aotate Control Imn 2mn 3mn c WO E E se Change Tip Position 2 axis Donor Control EE se 2 anis Tip Control ND NE Safety Zone Sampling Set Try Thrust BEN se DREI se EN ze Plunger Position CL Set Speed select e p Plunger lt Fig 5 3 45 gt Tip Rotate Ctrl The location of each tip can be defined Each of the tips is separated for approximately 120 degree and this tip is rotating to use the desired size of the tip If the rotation location is to be changed the text box is to be input with numbers by using the touch pad and make sure to press the Set button to save and apply the value ds 29 UNITMA Quick Ray Master UATM 272 Operating Manual Change Tip Pos Z axis Donor Ctrl Z axis Tip amp Recipient Ctrl Plunger Pos When the button is clicked it moves to the ready location
46. icrotome About 4um ear na lt Fig 5 1 6 gt lt Fig 5 1 7 gt e 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 2 Preparation for punching and inserting with Quick Ray Master Keep the front door of the instrument at a safe distance from your head and face Be careful about your fingers or hand not to be scratched by the needle of tip when assembling or disassembling the puncher or block trays or the Main plate or when cleaning the interior of the instrument or the tip Be careful not to pinch your fingers when closing the front door of the instrument 5 2 1 Fit donor blocks and a recipient block into the Main plate in Quick Ray Master 5 2 1 1 Putting the recipient block into the recipient block holder 1 Putting the recipient block 1 into the recipient block holder top 2 2 Fitting the recipient block holder top with the recipient block into the recipient block holder body 3 3 Turns the adjust screw 4 to the lock direction for tightening up the recipient block 1 to recipient block holder top 2 to the end 4 Fitted recipient block holder lt Fig 5 2 1 gt 5 2 1 2 Fitting the pre marked donor block into the donor block tray Points should be marked on the donor block with oil ink where the user intends to extract the sample tissue from and insert it into the donor block tray the points should be marked at least 2 hours before the implementation for the oil ink dry up c
47. ife threatening disease for which there is no vaccine or other treatment available Practices and Techniques Safety Equipment Bio safety Level Standard microbiological practices special laboratory clothing controlled access Level 3 practices plus airlock system negative air pressure 11 Level 1 practices plus Laboratory coats decontamination of all infectious wastes limited access protective gloves and biohazard warning signs as indicated Level 2 practices plus Practices and e See None primary containment provided by adherence to standard laboratory practices during open bench operations Partial containment equipment i e Class I or II Biological Safety Cabinets used to conduct mechanical manipulative procedures that have high aerosol potential that may increase the risk of exposure to personnel Containment Partial containment equipment used for all manipulations of infectious material Maximum Maximum containment Containment equipment 26 UNITMA Quick Ray Master UATM 272 Operating Manual 3 INSTRUMENT COMPONENTS AND SPEGIFICATIONS 3 1 Instrument description Tissue Microarray TMA Tissue Microarrays are a collection of multiple tissue cores that are arranged in columns and rows inside a paraffin block allowing for histological analysis They are a crucial tool in the analysis of gene and protein expression levels in samples from normal and di
48. ights reserved Licensed products are owned by Unitma Co Ltd and are protected by national copyright laws and international treaty provisions Unitma Co Ltd products are covered by patents issued and pending Specifications and price change privileges reserved Quick Ray is registered trademarks of Unitma Co Ltd All Information is subject to change without prior notice Contact to Unitma Co Ltd 3F Chungmyeong B D 224 8 Jamsil Dong Songpa Ku Seoul 138 220 Korea Tel 82 2 420 0070 Fax 82 2 420 9797 http www unitma com E mail unitma unitma com 26 UNITMA Quick Ray Master UATM 272 Operating Manual Table of contents 1 IMPORTANT NOTIES 5 1 1 Explanation of Symbols used U 5 IO KE Ce CD uu OU 6 1 9 Qualification of personnel 6 1 4 Intended use of the instrument 6 2 SAFETY NOTES 7 3 INSTRUMENT COMPONENTS AND SPECIFICATIONS 12 3 1 Instrument AESENPUON DEE 12 3 2 1NSUUMENLEOMPONENIS E 13 3 3 Instrument specifications 16 3 4 Consumables Recipient block EEN 17 4 SET
49. is lifted up to make the next motion Manual page 1 Tip Up After finishing the tissue insertion the tip positioned on the recipient block surface is lifted up to make the next motion Manual page 2 To move to the Manual page 2 And it is inactivie on Manual page 2 i 99 UNITMA Quick Ray Master UATM 272 Operating Manual Motion Controller Plunger N A Pa owe ai Ed Fig 5 3 23 This part Fig 5 3 23 gt is the motion controller Speed select is to apply the speed set at the Set mode Position displays the position of each axis The plunger tip controls the needle ATC may modify the rotation position of the tip The Red arrow button may control the X and Y axis and the Blue arrow button located in the middle of the X and Y axis controls the Z axis for Cam 1 Cam 2 and Tip Stop button is to suspend all motions not intended 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 2 4 Teaching When the Teaching button is clicked the screen is changed as shown in lt Fig 5 3 24 gt In addition in the event that mark finding is failed during the process implementation and when the location of the mark is set by the user definition the screen is changed as follows E Quick Ray Column l 2345 6 gt lt 29 y moy Mark amp Hole Position Setting Setting Gab 8 Recipient Recipient Block Donor Block BEN move
50. isposal of Tip waste 1 The tip waste must be contained in a special sharp object container 2 The waste with specimen shall be placed in a red biohazard bag d 99 UNITMA Quick Ray Master UATM 272 Operating Manual 7 TROUBLE SHOOTING 7 1 Instrument failures Some failures or malfunctions of the instrument are indicated by the applicative software as error message Follow the software instructions to solve the problems and take corrective actions 7 2 Operating error with possible causes and corrective action It is Not selected hole When running the Auto Mode neither the Donor block nor the Recipient block hole has been selected during the Set mode Please refer to section 5 3 2 3 SET menu of this operating manual It is already registered with hole When during the SET mode the hole of the Recipient block has been selected two times this message will show Please select another hole on the Recipient block to receive the tissue specimen La I Nothing to extract from a Donor at When using the Set Mode the maximum this time number of punching holes from Donor block is 40 This error message will disappear automatically and no more selection will be accepted Selected All Hole When the available number of holes in the Recipient block has been filled and no more tissue specimens from Donor blocks can be accepted Do Initialize To run the instrument initialization from Auto mode must be selected W
51. lecting white color on the donor blocks In case the instrument fails in differentiating between the colors on the donor blocks and the colors at the bottom of vacant donor block hole extracted the sample tissues the program will stop to return to Teaching Mode Select the Select Recipient Block All UATM 272 is designed Recipient ORI AecipA 6 ecip 6 Copy EW to load two recipient blocks Block simultaneously and the tissues are inserted in the sequence of A 1 A 2 and A 3 of recipient block A and when the insertion up to N N is completed it moves to insert at A 1 of recipient block B Please make sure that two recipient blocks shall be the same whole size Copy The tissue extracted from the same donor block can be inserted in the same location of each different recipient blocks For example the sample 29 UNITMA Quick Ray Master UATM 272 Operating Manual collected from the first donor 1 is inserted in the location of A 1 of recipient block A and the tissue collected from the second donor block 1 has to be inserted to the location of A 1 of recipient block B At this time the punch count must be even number RecipA To insert the sample tissues into the only recipient block A If a recipient block A is inserted completely replaced the recipient block A with new one to continue its execution RecipB To insert the sample tissues into the only recipient block B If a recipient block B is inser
52. n cause electrocution burns fire and other HAZARDS READ THIS BEFORE USING THE INSTRUMENT 1 Check that the voltage setting matches the supply voltage 2 Connection to MAIN POWER SUPPLY a For plug connected instrument only Where protective ground is required plug the instrument into a supply outlet which has an earth connection b For PERMANENTLY CONNECTED INSTRUMENT only Do not use the instrument until it has been installed by a qualified electrician or authorized service engineer 3 Unplug the instrument immediately after use 4 Unplug the instrument before filling with liquid 5 Do not place the instrument in liquid nor put it where it could fall into liquid If the instrument becomes wet unplug it before touching it 6 Do not leave the instrument unattended while it is plugged in 7 Use the instrument only for the purpose described in the instructions for use 8 Do not use accessories which are not supplied or recommended by the manufacturer 9 Do not use the instrument if it is not working properly or if it has suffered any damage NOTE Examples of physical damage include a damage to the flexible supply cord or its plug b damage caused by dropping the instrument c damage caused by dropping the instrument into water or splashing water onto it 10 Do not let the instrument or its flexible cord come into contact with surfaces which are hot 11 Do not block air openings or place instrument on a
53. nd accessories according to the instructions in this manual Neither authorization for the analysis or modification of the instrument nor the analysis and use of its circuit configurations is provided Never disassemble the instrument or supplied accessories Disassembling will void the instrument warranty Additionally there is a risk of fire electrical shock or malfunction Do not use accessories that are not supplied or recommended by UNITMA Contact UNITMA for repairing service or to purchase accessories Do not use the Donor block or a sample with a biological hazard or a chemical hazard Do not use the donor block or a sample with biological hazardous substances and or chemical hazardous substances that can impact human health To avoid puncher tip a damage A Keep the puncher tip clean and do not use the instrument if there are foreign things Silk fiber Stapler pin Calcification tissue Bone and Teeth in the puncher tip Handle biohazards Working with infectious material requires the following precautions a Limit access to areas where experiments with infectious specimens are in progress b Clearly label areas where biohazards are in use and designate specific areas where biohazards are routinely used using this symbol black on red background LO c Wear lab coat gloves and safety glasses to prevent contamination from the infectious specimen and remove them when leaving the work a
54. no IER move Row BE on col BE 1 12 Speed select Donor Set This button is always inactivated when clicking the Manual button whereas it is activated when failing to read the donor block mark during the process At this time the user can choose the position to extract the tissue on the donor blocks The user can choose the desired position with the mouse or the direction key located in between the Donor Set button and the Recipient Set button k 29 UNITMA Quick Ray Master UATM 272 Operating Manual Recip Set This button is also always inactivated at the time of clicking pg Manual button whereas it is activated when failing to read as the recipient block hole during the process At this time the user can choose the position to extract the tissue on the donor blocks The user can choose the desired hole with the mouse or the direction key located in between the Donor Set button and the Recipient Set button Direction key This button is used to set the mark location of donor or the center of the recipient block hole This is the button to adjust the location properly rather than by the touch screen Y After the user set the location with the touch screen the user may adjust the location in more detail by using this button The Gab in the direction key displays the number of pixel moved by pressing each time Recipient Recipient Block Press the Move button as in the Manual mode to move
55. ompletely The user can choose one of three colors White Red and Black which can be contrasted by the color of sample tissues Please refer to the additional explanation shown as lt Fig 5 3 7 gt e 99 UNITMA Quick Ray Master UATM 272 Operating Manual lt Fig 5 2 2 gt 5 2 1 3 Putting the trays into the Main plate Putting the donor block tray 1 and recipient block holder tray 2 into the Main plate lt Fig 5 2 3 gt 5 2 1 4 Procedure of fitting the block trays into the Main plate of the instrument 1 Turn on the system 2 Start AUTO mode 3 Load spread sheet file and click on Set Tray button 4 Open the tray door i lt Fig 5 2 4 gt 5 Putthe donor block tray and recipient block holder tray into the Main plate of the instrument The jig in the main plate of the instrument should not be disassembled from the instrument UNITMA Quick Ray Master UATM 272 Operating Manual lt Remove donor block tray gt lt Remove recipient block holder tray gt lt Fig 5 2 5 gt lt Fill the donor blocks and recipient blocks in each tray and put the trays into the Main plate gt 6 Close the tray door 5 2 2 Remove the recipient blocks from the Quick Ray Master 1 Wait until the implementation of the instrument is completed or stopped The user can hear a music sound from the instrument when the instrument stopped 2 Open the tray door 3 Take out the recipient blo
56. on the users can appoint the area intended to extract the sample tissue on the Donor block and then press the OK button to return Auto mode In another case of failure in recognizing the hole position of recipient block during the automatic execution the users can manually select the hole intended to insert the sample tissue Refer to section 5 3 2 2 4 Teaching di 29 UNITMA Quick Ray Master UATM 272 Operating Manual Main Menu To move to the main menu To exit from the program Enit Button Description of function Load file To download the Excel data file for the saved information of donor Load blocks Core size Punch count Sample count and others Load log l In the event that the Suspend button is clicked to stop the program this button function is to start again to download the data used previously Initialize ei Initialize To undertake the function to find the original position of each axis motor Set Tray o Set Tray To move the main plate to the tray door for each donor block or recipient block to be replaced Select the To select the color to be marked the mark color select Donor Mark Color desired point on the donor block One OUhite Black R ed on Donor color can be selected from white black blocks and red and the color of donor blocks in an Excel file has to be marked with the same color In case the cassette color is white please make sure of avoiding se
57. p cam Cami Dist S Speed select Stop how Middle Migh ert Plunger lt Fig 5 3 50 gt Select Model The tip size can be selected from 1 2 and 3mm Once the tip size is selected the location is automatically arranged to the applicable tip Move Tip Pos It moves to the Cal Tip pos as previously set in the 5 3 2 3 2 p 29 UNITMA Quick Ray Master UATM 272 Operating Manual Recipient Block Param Next the user uses the motion controller on the lower part to move to locate the tip in the middle of the calibration mark Register tip pos The location to move from the Move tip pos is memorized Move CAM 2 Following the Select Model it moves to the calibration position as Calibration Pos already set at 5 3 2 3 2 Recipient Block Param The location is prudently set to be located slightly to the northeast part from the center as described in the earlier paragraph This is designed to prevent the phenomenon of the mark deviating from the camera FOV Field of View UNITMA Quick Ray fave Lama Lallbratign Pos Calibrate ama Register Cal distance tip came Move Cam Calibration Fos Calibrate Cami Register mn TL wer Cal distance tip cami Speed select lt Fig 5 3 51 gt Calibrate CAM 2 This is to calculate the pixel resolution of the camera At first the movement is made toward X axis direction by 0 5mm then it moves to X axi
58. rea d Decontaminate work surfaces once per day and after any spill of viable specimen e Eating drinking and applying cosmetics are not permitted in the work area f Wash hands after handling viable specimens before leaving the lab g Transport contaminated materials in leak proof containers clearly marked with biohazard labels i 29 UNITMA Quick Ray Master UATM 272 Operating Manual Biohazard Determination Lab Bio safety Level BSL Criteria The following guidelines can be used by all laboratory personnel a Bio safety Level 1 is appropriate for undergraduate and secondary educational training and teaching laboratories and or other facilities in which work is done with defined and characterized strains of viable microorganisms not known to cause disease in healthy adult humans b Bio safety Level 2 is applicable to clinical diagnostic teaching and other facilities in which work is done with the broad spectrum of indigenous moderate risk agents present in the community and associated with human disease of varying severity c Bio safety Level 3 is applicable to clinical diagnostic teaching research or manufacturing facilities in which work is done with indigenous or exotic agents where the potential for infection by aerosols is real and the disease may have serious or lethal consequences d Bio safety Level 4 is applicable to work with dangerous and exotic agents which pose a high individual risk of l
59. s direction Y axis direction and Y axis direction and the applicable pixel distance is calculated for the central point of each mark to formulate the resolution by the proportion And the location is arranged to have the mark center to come into the central part to calculate the distance between the tip and camera 2 2 29 UNITMA Quick Ray Master UATM 272 Operating Manual Quick Ray Cal distance tip ont Move Cami Calibration Fos es Cami hegister emm am ner Cal distance tip cami col 1 Save Speed select Stop s tow made mm io Plunger DN 4 LB a menu lt Fig 5 3 52 gt Cal distance tip If this button is pressed it calculates the distance from the tip location CAM 2 calculated earlier and the focused location of the camera 2 Move CAM 1 By following the value set from the Select Model Camera 1 moves to Calibration Pos calibration position mark as set in the 5 3 2 3 2 Recipient Block Param in advance Fa E I ECH Move fama Calibration 05 Calibrate Cam iat ms 2 Esc D r Donar ial distance tp Z cami aE Caml talleration Fas Calibrate Cami Register Cal aR pem aum cami Save speed s select lt Fig 5 3 53 gt 5 29 UNITMA Quick Ray Master UATM 272 Operating Manual Calibrate CAM 1 The pixel resolution of the camera is calculated At first the movement is made toward X axis direction by
60. seased specimens Further they are useful in the early stage discovery of gene targets in genomic research validating targets testing and optimization of diagnostic tests and in the quality control of molecular detection schemes And the Tissue array technology not only makes decrease the reagent time and human resource below one sixtieth but also can be applied to most of the know how about tissues for immunohistochemistry in situ hybridization FISH and in situ PCR e Quick Ray Master UATM 272 The instrument Quick Ray Master UATM 272 is an automated tissue microarrayer for extracting tissues from donor blocks and subsequently delivering them into the recipient block The Recipient block UB06 the patented technologies of UNITMA is made of special materials that melts when heated at 60 C for 30 minutes and has pre made evenly spaced round wells arranged in a square matrix Therefore using UNITMA recipient block will save the conventional block building time and guide the pathologists to fully utilize the power of TMAs The instrument uses these ready to use pre made recipient blocks helping to decrease the time and labor needed for block creation In addition because it has one tip module that holds 3 tips hole size 1mm 2mm and 3mm which can automatically be changed simply by using the software there is no need for disassembling or assembling punch modules when changing the hole size The instrument provides an automatic mo
61. set Tray Ponor ar Mark color nic Quite black Sid Select Recipient Black Oni GredpA d heciph Copy T T Manual ing w Sg d S suspend GLED 4 lt Fig 5 3 5 gt TM the user presses this button to confirm the insert status on i AE the recipient block and if the hole does not have the tissue inserted the color of the recipient block hole on the right upper part of the screen turns from green to red and the error message is shown on the Excel data file at the same time If the user presses this button to confirm the extract status W DIC from the donor block the user can check if the donor block is lifting entirely or cracked In this case the instrument returns to Pause stage with pop up message box 5 3 2 2 2 Program implementation Program Turn on the main power switch on the right side of the instrument implementation and then Turn on the power switch for PC Wait until the window booting is completed and click on the base screen of window i 29 UNITMA Quick Ray Master UATM 272 Operating Manual Finding the oo ai Ula position to 929 PET initialize Imm T 120 cores 2mm bU cores acie 3 mm 30 ores Quick Ray lt Fig 5 3 6 gt When the program is loaded as shown in lt Fig 5 3 6 gt the finding initial Seele Is mple mentee together initialize Press the button to implement the finding initial position AUTO mode Be Quick Ra
62. soft surface which might block them and keep air openings free from lint hair fluff etc 12 Do not place anything on top of the instrument 13 Unless specifically instructed to do so by this operating manual do not drop or put anything into any opening in the instrument or into any hose or coupling 14 Do not use the instrument where aerosol sprays are being used or where oxygen is being administered 15 Do not use the instrument out door 26 UNITMA Quick Ray Master UATM 272 Operating Manual To avoid fire and the instrument failure Observe all warnings and instructions Observe all Terminal Ratings To avoid fire or shock hazard observe all ratings and markings on the product Consult the product manual for further ratings information before making connections to the product Power Disconnection The power switch disconnects the product from the power source See instructions for the location Do not block the power switch it must remain accessible to the user at all times Regularly inspect the AC power cord for damage and for dust build up around the power plug or electrical outlet Stop using and unplug the AC power cord from the electrical outlet and disconnect any other cables immediately in the following cases when the instrument functions in an abnormal manner produces unusual sounds if there is unpleasant or offensive odor and becomes too hot to touch Do not operate when the front
63. sue When pressing the push button from the Manual mode it displays the location of the P axis The location can be modified by using the Touch pad and make sure to press the Set button to save the modified location p UNITMA Quick Ray Master UATM 272 Operating Manual 5 3 2 3 4 Speed Parameter If the Speed Param button is clicked following screen Fig 5 3 46 gt will be displayed The user can define the speed of each motor SS UNITMA Quick Ray Fig 5 3 46 Load amp Save The Load button brings the data saved to apply The Save button saves the value already set Pe 29 UNITMA Quick Ray Master UATM 272 Operating Manual It defines the speed when the Low button lt Fig 5 3 47 gt is pressed at the Speed Select of the motion controller It does not give any influence on the tack time When the speed is to be modified the touch pad is used to input the number on the text box and make sure to press the Save button to save and apply the value lt Fig 5 3 47 gt It defines the speed when the Middle button lt Fig 5 3 48 gt is pressed at the speed select of the motion controller It does not give any influence on the tack time When the speed is to be modified the touch pad is used to input the number on the text box and make sure to press the Save button to save and apply the value lt Fig 5 3 48 gt
64. t shows the result of completion for the TMA process lt Fig 5 3 19 gt The data of block completed with the work has the same type with the recipient block shape as shown in the following lt Fig 5 3 20 gt and it is saved in the Excel file including the tissue ID inserted in the hole of recipient block i 29 UNITMA Quick Ray Master UATM 272 Operating Manual L Fig 5 3 20 5 3 2 2 3 Manual When the Manual button is clicked the screen changes as in lt Fig 5 3 21 gt The user can operate the instrument step by step manually for all procedures as implemented by AUTO mode At this time the data has to be saved on the Excel file separately the marking of recipient blocks RIC and DIG would be excluded The Manual mode is configured with a total of 2 pages and Manual page 1 undertakes the motion related to the donor block and Manual page 2 undertakes the motion related to the recipient block It is useful in the event that the device fails in recognizing the marks on the donor blocks or in extracting the sample tissues from the donor blocks during the execution at the AUTO mode In addition since the each motion of the AUTO mode is implemented by each step so that it is great to understand the sequence and concept of the entire process However in the event that the unskilled person follows this procedure it may cause damage to the instrument so that the user undertakes carefully k
65. ted completely replaced the recipient block B with new one to continue its execution Start afi To implement the serial process of sample tissues Start extraction and insertion When the user presses the EMG Stop at the emergency situation and wants to restart the following pop up will be displayed on the window SS UNITMA Quick Ray Columnil 2 3 454 gt Recipient Hole l 3 ae e es e a 66683866 6 e a WS WWW Pnitialize SP Set Tray Donor Mark Color Bred Se o o 9 49 9 8 6 555959 5 5 99000009 Recipient Block md OG Bech ecp Copy BC estat CL Fig 5 3 4 gt When the OK button Fig 5 3 4 gt is clicked after defining the donor block punch count recipient block and location of recipient block hole the process is re implemented me s To stop temporarily Suspend S Io suspend the program After completing a series of i extraction and insertion of the sample tissues the log file will be prepared and saved automatically To restart the program later the Load log button is used to load the saved data EMG Stop GE In case the users meet any abnormal situation the user M EIS EOP can press EMG Stop to stop the program with the music sound then click the Complete button to initialize the original position automatically 32 26 UNITMA Quick Ray Master UATM 272 Operating Manual ToS Unirma Quick Ray tialize D
66. the front door of the instrument Use in a well illuminated area and keep the LCD screen in a safe distance from your face Keep the front door of the instrument in a safe distance from your head and face Do not operate without covers Do not operate this product if covers or panels are removed Keep the instrument and accessories out of the reach of children Do not touch the plug of the AC power cord with wet hands Be careful with your fingers or hands from being injured while assembling or disassembling the tip body or the block holder frame or when cleaning the interior of the instrument AC power cord use and Ground Use Proper Power Cord Use only the power cord specified for this instrument and certified for the country of use Verify the voltage and frequency of AC power source Ground the instrument This instrument is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making connections to the input or output terminals of the product ensure that the product is properly grounded When disconnecting the AC power cord hold it by the plug and pull straight out form the electrical socket Never pull by the cord or pull at an angle 26 UNITMA Quick Ray Master UATM 272 Operating Manual Use the instrument and accessories according to the instructions in this manual Use the instrument a
67. the fuse holder back into the fuse holder module 7 Snap the cover of the fuse panel 6 5 Repair A Please contact UNITMA for repairing services replacement of components and additional consumables 6 6 Disposal of waste e Disposal of waste from Donor block or Recipient block with tissue specimen 1 The waste with tissue specimen should be contained and handled separately from other wastes at the point of generation 2 The waste with specimen shall be placed in a red biohazard bag 3 Red biohazard bags are to be tied 4 Red biohazard bags are to be placed for storage handling and transport in rigid containers with tight fitting lids labeled with the words Bio hazardous Waste or the word Biohazard and the international biohazard symbol on the lids and sides so as to be visible from any lateral direction di 29 UNITMA Quick Ray Master UATM 272 Operating Manual 5 Pathology Waste All pathological wastes must be separated from other medical hazardous wastes Waste will be placed in a red bag and deposited into a specially marked secondary container labeled with words Pathology Waste or PATH The container will be stored in the morgue refrigerator until pick up by authorized medical waste hauler for transportation to an approved incineration facility Once specimens or tissues are deemed waste they shall not be stored for more than seven 7 days at a temperature above 32 degrees Fahrenheit e D
68. tion may be modified by using the touch pad and make sure to press the Set button to save the location modified lt Fig 5 3 39 gt gt This above picture lt Fig 5 3 39 gt is camera 1 position within the instrument when the Cal pos CAM 1 button is clicked GS UNITMA Quick Ray He ba p Position Info ef AR GER RN i TT m mM Standby Pc Recipient Info Select model SIE Recipient 11 SSES ES set Recipient 2 BEES FOE set Exe ES set lt Fig 5 3 40 gt a UNITMA Quick Ray Master UATM 272 Operating Manual gt Screen lt Fig 5 3 40 gt shows captured image when the button is clicked Calibration 1mm Mark is made to move to the central part of the CAM 1 screen 99 UNITMA Quick Ray Master UATM 272 Operating Manual Cal pos cam When the calibration of Camera 2 is made the location can be designated When this button is clicked in the following figure lt Fig 5 3 41 gt it moves to the designated location The location may be modified by using the touch pad and make sure to press the Set button to save the location modified As in the above figure the tip has to be located around the center of the calibration mark lt Fig 5 3 41 gt gt This above picture lt Fig 5 3 41 gt is Tip calibration position of inside of instrument when the Cal pos 2mm button is clicked CS UNITMA cl AW enge Quick
69. y Columnli 2 3 4 5 amp Ka Punch ent Res ei initialize D Set Tray Select Donor Mark Color QWuUhie epBlxk Ghed Fig 5 3 7 gt Click the AUTO button from the main menu to turn the above screen Load the file Load the data file for the donor block input in advance x fie x Following figure lt Fig 5 3 8 gt is the example of the data file Core size sample count and punch count should be accurately defined and the applicable donor block data is prepared accordingly m UNITMA Quick Ray Color selection to be marked on the donor block Designation of the recipient block to be used Set Tray Master UATM 272 Operating Manual Tissue Array Res Block Menider Tex Age Fiscal om Organ TZ Type ornam 70 0 0909003587098 K K K K 88 k lt Fig 5 3 8 gt T h k h Select Donor Mark Color o select the color marked on the OUhite Black R ed desired point of the donor block One color can be selected from white black and red and the color of donor blocks in an Excel file has to be marked with the same color In case the cassette color is white please make sure of avoiding selecting white color on the donor blocks In case the instrument fails in differentiating between the colors on the donor blocks and the colors at the bottom of vacant donor block hole extracted the sample tissues the program will stop to return to Teaching Mode Select ecipient Block S

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