User Manual of AgileCycler
Contents
1. Figure 1 Frontal view of Thermal Cycler AgileCycler e Reaction module bay holds reaction module e Air vents allows air circulation e Status LED indicates status of reaction module e LCD display displays operating status e USB A port 2 USB ports connection for computer mouse and USB devices Connector Locking screw Fuses l USB B port Power switch Power input Test port Ethernet port Figure 2 Rear view of thermal cycler e Connector connection between host machine and reaction module e Locking screw locks reaction module e Test port for service testing only e Ethernet port connection to computer e USB B port for system debug Note 1 The fuse specifications are 250V 8A q5x20mm Use only the fuse that meets the above specifications For certificated fuses please contact distributor 2 Forexchanging block please consult your distributors AgileCycler Lid knob P st Lid pull 8 ff Connector Cooling fins nn Figure 3 The lid and cooling fins of 96 well reaction module e Lid knob to seal Lid e Lid pull to open and close lid e Connector connection between host machine and reaction module Inner Lid Reaction block Figure 4 Opening view of a 96 well reaction module e Reaction block holds reaction vessels including tubes and microplates e Inner lid applies heat and force to the reaction vessel lids cap2. Biological programmatic and hardware problems may contribute to any unsatisfied results of experiment In order to distinguish the hardware problems from other possible 25 AgileCycler problems the machine is equipped with built in self test hardware and self diagnosis software Below is the detailed description According to the experiences most problems are related to biological and programmatic factors 1 Incorrect or insufficient reactants 2 The temperature is too high or too low It is suggested to set the temp within range of 90 95 0 for 40 seconds The duration can be adjusted in accordance with reaction volume 3 Annealing temperature is too high or too low It is better to between 55 70 C and 20 to 30 chains 4 The reactant concentration is too high or too low 5 Lack of special treatment during preparations 6 Time and temperature programming are not appropriate 7 Sample temperature is slightly too low and the block temperature is slightly too high 8 Check whether the PCR tubes are well placed Smearing little mineral oil on the surface of holes will increase the thermal conductivity 2 2 Self test hardware and self diagnosis software The machine will run the self test program when booting 3 Notices 3 1 Power 1 No special requirements for the power source Any AC power source within range of 85V 264V is applicable However to prevent from causing any damage to the machine it
3. 1 1 Boot Connect power cord to the back of instrument then plug into power source and switch to When instrument is on a buzzer should be heard and Boot screen Figure 5 will appear on LCD screen The operating system then goes to Self Test screen Figure 6 During this phase the LCD screen will display instrument model and company LOGO Home screen Figure 7 will be shown when starting up phase is completed THERMAL CYCLER AgileCycler Self test please wait Figure 5 boot screen Figure 6 self test screen AgileCycler 12 06 05 17 05 02 Infinigen AgileCycler Setting Incubate Help Figure 7 home screen 1 2 Warm up In standby modes the instrument will pre heat the Block and Hot Lid Please refer to Run Setting for setting instructions of Hot Lid work mode The default Hot Lid pre heating procedure is carried out once the power is on and the default temperature is 105 C 10 AgileCycler 2 Menu structure Home File Setting Tools Incubate Help New Edit Run Print Options Copy Paste Delete Rename Back Run setting Hot lid Temp Hot lid work mode Lid auto closed Temp Temp control System setting Time Date Language sound Gradient calculator Logs Local information AgileCycler 3 File File is composed by Temperature Step and Cycl
4. 4 SENE m H 19 Ale RUM SEINE EO 20 42 51518611156 111 21 5 TOON EO ay 22 Sl 950607 COCOONS LU 22 92s MM 23 oe Locali orma ON ae a E oi caer ceo cO pads 23 AgileCycler CHAPTER 3 1 1 1 1 2 2 2 1 2 2 3 3 3 2 3 3 MAINTENANCE amp TROUBELSHOOTING 015110161981166 eee 0188101118 e ES AR Replace 056 SOLS ease TROUDICSHOOUNG cuc o eite eres e De p Nea EE Dissatisfactory Results eoe etre Self test hardware and self diagnosis software INOLICOS ances ede NOTICE ON CIEARINE tec Error message amp solution 0 0 Cause of the abnormal phenomenon amp solution AgileCycler CHAPTER 1 INSTALLATIONS 1 The AgileCycler Color Touch Thermal Cycler package includes AgileCycler Color Touch Thermal Cycler Power Cord 2pieces of 8A 250V fuses e Instruction manual 2 Normal Operating Conditions Ambient temperature 10 C 30 C Relative humidity lt 70 Power source 85 264V 50 60Hz 600W Note Please check if the above operating conditions are met before turning the power on Pay special attention to power line s grounding 3 Transportation and Storage Ambient temperature 20 55 C Relative humidity lt 80 4 Parts and Functions Status LED Reaction module bay LCD display Air vents USB A port
5. 05 02 596 62 3 64 1 O30 450 458 6 50 0 52 2 0 55 5 3 1 2 3 4 5 6 7 8 9 f Temperature EI 1 Gradient 18 gt Figure 23 Gradient calculator oe AgileCycler 5 2 5 System logs name running starting time and times of test for each file Maximum 1000 logs can be recorded and the system will remove older logs once the capacity is excess Figure 24 12 06 05 17 05 02 2012 6 5 18 01 ST3 TD 2012 6 5 17 37 Grad td 2012 6 5 15 41 GraDF td 2012 6 5 14 12 t3 td 2012 6 5 14 12 t3 td 2012 6 5 14 07 t3 td 2012 6 5 14 00 t3 td 2012 6 5 13 29 t3 td 2012 6 5 13 22 t3 td 2012 6 5 12 49 t3 td 2012 6 5 11 44 t3 td 2012 6 5 11 33 t3 td 2012 6 5 11 25 t3 td 2012 6 5 11 05 t3 td 2012 6 5 10 32 t3 td 2012 6 5 10 24 t3 td 2012 6 5 10 22 t2 td 2012 6 5 10 20 ti td 2012 6 5 10 12 t1 td 2012 6 5 10 07 TCHDOWN TD Figure 25 Local information 5 3 Local information Show the IP address and block type Figure 25 Local Information 12 06 05 17 05 02 IP address 192 168 55 101 Block type 96x0 2ml Figure 24 Run logs 208 AgileCycler 6 Incubate Touch Incubate button in the Home screen to enter Incubate Setup screen Figure 26 Incubate Setup 12 06 05 17 05 02 1 Select Block f A Block Idle f B Block Invalid 2 Block Temp Setting c Cancel Figure 26 Incubate setup Incubation function maintains steady block temperature for unlimited period
6. First select the desired blocks in the Incubate Setup screen Figure 26 and enter block temperature then touch Start button to enter Incubate Running screen Figure 27 A Block Running 12 06 05 17 05 02 Block Temp 0 Lid Temp 105 0 C Figure 27 Incubate running NL AgileCycler CHAPTER 3 MAINTENANCE TROUBELSHOOTING 1 Maintenance 1 1 Cleaning e Clean the holes on reaction block with neutral soap solution Do not use any solvent contains alkali alcohol or organic solution e Spaces near the left right air vents and under the machine should be always kept free It is important to clean the dust around air vents regularly Clean reaction block regularly and remove any residue inside cavities to prevent any affects to the temperature control soft cloth advised e Soft cloth can also be used to scrub the dirty spots of the machine surface Warning Turn off the power during cleaning Corrosive solvent is not allowed for cleaning the surface 1 2 Replace fuse The machine has two fuses Once damaged please replace damaged fuses with following steps 1 Turn off the machine and unplug power source 2 Unscrew the fuse box with flat screwdriver and replace the damaged fuses with new 8A 250V fuses After replacement screw back in the fuses box A Attention please contact your local distributors when encountering any problem replacing fuses 2 Troubleshooting 2 1 Dissatisfactory Results
7. See Se Temp Cycle Delete Options Figure 10 Edit an old file In the Edit File screen Fig 10 select a desired step by touching anywhere in the step Change a time or temperature by touching the desired field and entering a value using the pop up numeric keypad To enter an infinite hold fill time field with 00 00 2 Edit Step Select a desired step touch Options button to enter Step Options screen Figure 11 Parameters can be edited by selecting among Gradient Ramp Rate Temperature Increment or Time Extend Table 1 lists the parameters for temperature and gradient steps with the limits of those parameters After entering Gradient values the gradient distribution table Figure 11 will be shown at bottom of the screen Touch OK to save changes and return Edit File screen Figure 12 ie AgileCycler Step Options 12 06 05 17 05 02 Temperature 55 0 1 The target temperature between 0 0 and 100 0 C in Gradient G Increment C Cycle Ramp Rate C Sec Time Sec Extend 6 Sec Cycle tenths of a degree Gradient 1 2 3 4 5 6 7 8 9 10 11 12 99 0 55 4 56 3 57 5 586 59 5 60 3 61 2 62 3 63 7 646 65 0 gt Figure 11 Set the Gradient Edit File 12 06 05 17 05 02 I LESE x 29 Temp 55 0 Increment 0 0 C Cyc Gradient 10 0 C Time 00 30 Extend 0 s Cyc Ramp Rate 0 0 C s Temp Cycle Options Figu
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9. in between Do not place the device on a soft surface which may block the ventilation on the bottom of device Hot environment may degrade or failure on of the Thermal Cycler Therefore the device should be protected against any kind of heat sources such as sunlight ovens or central heating equipment When Thermal Cycler is to be in idol for long period of time it is recommended to disconnect the power and cover up the device to prevent dust from entering Note Immediately disconnect the power and contact the distributor or consult certified mechanic for assistance when any of the following occurs e Liquid gets into device The device is sprinkled or drenched e The device malfunctions producing abnormal sound or odor e The device falls down to the floor or outer shell is damaged e Significant changes in the device s performance 3 Protection Degree IP 20 AgileCycler 4 Warning Sign Icon Meaning High temperature warning A Areas labeled with this warning signs and those described in the manual Do not touch to avoid getting burned Electric shocks Warning Operate strictly in accordance to the to avoid electric shock accidents Attention A Important message Read the message carefully Failure to do so may cause machine malfunction or damage to the machine WARRANTY AND SERVICE INFORMATION 1 Warranty The Thermal Cycler is warranted for one month starting from date of ship out Under this war
10. lid on when power on Hot lid on when file running File start when lid Temp attained 3 Lid heater auto shutoff Temp G 4 Temp Control Block control mode Tube control mode gt Cancel Figure 20 Run parameter setting 1 Hot lid temperature setting Hot lid temperature setting range is 30 110 0 and system default temperature is 105 C 2 Hot lid work mode setting In total are 4 working modes and default mode is that the hot lid will be on when power is on 3 Lid heater auto shutoff temperature The range of lid heater auto shutoff Temp is 0 100 C and system default is 25 C When the block is running an infinite hold at a temperature which is lower than the _ 20 AgileCycler specified auto shutoff temperature the lid heater will turn off and the lid temperature will stay at block temperature Note Lid Temperature The adjustable heated lid of AgileCycler allows user to control the lid temperature and force When AgileCycler is running the heated lid maintains the temperature specified by the file being run Without a heated lid water can be lost from the reagents to condensation concentrating the reactants in the tube or plate However if the temperature of heated lid is too high the sample temperature might rise above the target temperature Therefore pay attention to the setting of lid temperature could make your experiment result more reproducible 4 Temp control mode
11. 4 td test td t3 td 2 Temp 55 0 C Time 00 30 Inc 0 2 C ec Ext 3Sec Cyc 2 Alex Are you sure to delete file Billy System Preivous runs USB Flash 3i LAN users 6 Temp 12 0 C Time Forever Delete Rename Figure 15 Delete a file 3 4 Copy file Touch Options button in the File Library screen Figure 8 to select files and touch copy button Then enter the file path and touch Paste button to finish copy Os i AgileCycler 3 5 Rename file Touch Options button in the File Library screen Figure 8 and select file Then touch Rename button and enter new file name in the dialog window to rename files 3 6 Run file Select the file needed in the File Library screen Figure 8 and touch Run button to enter the Run File Setup screen Figure 16 Run File Setup 12 06 05 17 05 02 1 Select block A Block Idle lj B Block Invalid 2 Volume 10 Cancel Figure 16 Run file setup Then select the Block and enter volume not necessary to be in block temperature control mode Touch Start enter the File Running screen Figure 17 A Block Running File ST3 12 06 05 17 05 02 PE NEN 950C 950 Start Time 16 53 m Remain Time 00 57 m Lid Temp 105 0 C Step 3 Step Time 00 30 s Block Temp 55 0 C Resume Stop Figure 17 File running 18 AgileCycler 1 Parameters e Start Time sys
12. Note The Infinigen Biotech Inc reserves all rights to modify this manual at any time without notice Please read this manual carefully before operating Thermal Cycler Document Version Jan 2013 Version 1 2 AgileCycler IMPORTANT INFORMATION 1 CAUTION Note The column contains important information please read it carefully Failure to follow can result in damage or malfunction of the Thermal Cycler Warning This symbol identifies certain cautions to be taken during operation procedure Failure to follow the requirements may result in personal injury 2 SAFETY PRECAUTION The following safety precautions should be taken during operation maintenance or repair of the Thermal Cycler to prevent any reduction on safety and affection on operation of the Thermal Cycler The Infinigen Biotech Inc shall not be in any way responsible for the consequences resulted from buyer s neglect to the following requirements Note The Thermal Cycler is only intended for indoor use and to operation under 2 000 meter elevation a Do not attempt to open the case of machine or touch any parts inside the machine with any tools b Grounding A C power needed to be grounded to prevent any electric shock Never let the third ground pin hanging loose If the 3 pin plug is not applicable it is recommended to consult electrician for appropriate power connection c Keep Away from Electric Circuits Opening Thermal Cycler is strictly p
13. The temp control mode contains block control mode and tube control mode The block control mode is suitable for performing normal PCR and the tube control mode is for experiments that require higher environment conditions The system default is block control mode 4 2 System Setting Select System Setting button in the Setting screen Figure 19 to enter the System Setting screen Figure 21 System Setting 12 06 05 17 05 02 1 Time Date 3 Sound Time E Keyboard sound Date 2012 6 5 gt Alarm sound ate 6 V File end sound 2 Language After reboot Temp to reach sound Chinese English Cancel Figure 21 System parameter setting 1 Time and date System time and date setting 2 Language System supports English only NU AgileCycler 3 Sounds e Keyboard sound sound when touch button e Alarm sound sound when system error occurs e File end sound sound when File running is complete e emp sound sound when the target temperature is reached 5 Tools Touch Tool button in the Home screen to enter Tools screen Figure23 12 06 05 17 05 02 1 Gradient Calculator 2 Logs 3 Local Information Figure 22 Tools menu 5 1 Gradient calculator The temperature value for each column is shown after entering the block target temperature and gradient range Figure 23 Gradient Calculator 12 06 05 17
14. al cycler Max temperature gradient 30 C Applicable with the following 0 2ml tube models non skirted semi and fully skirted plated Compatible with devices such as Mouse and Keyboard and capable to transfer data and perform software updates via USB Drive AgileCycler 7 Specifications Catalogue Number AC 96 AC 60 AC 48X2 AC 48N30 AC 384 TEN 10000 USB FLASH intuitive drag and drop program creation s Max No of Cycle of each step Time 1 Sec 9 Min 59 Sec Increment Decrement Temp NS Increment Decrement DI Connection to PC control Yes software AgileCycler Hold at 4 C Yes Printing Yes Wireless blue tooth Yes LAN to computer Yes LCD 8 inch 800x600 pixels TFT sensor feedback Dimensions 38cmx24cmx26cm LxWxH AgileCycler CHAPTER 2 OPERATION GUIDE Warning Please turn off the power immediately and contact your distributor when any abnormal sound or display appears after power on or when failure alarm and display are found during device s self testing process Note When sample number is less than available conical holes sample tubes should be evenly placed over the block to ensure the stable coverage and even temperature fluctuation distribution in blocks Note In order to guarantee that the heated lid is seamlessly sealed with the sample tubes and firm contacts between tubes and conical holes please apply light pressure onto the lid s flanks when closing 1 Power on Procedure
15. ching the targeted temperature CO 4 Slight pat or squeak sound while Normal The sounds are from the power switch machine is running mechanism when quick cooling or heating is needed Slow increasing or decreasing temp Check the settings of heating and cooling rate and check whether the fan is operating normally O1 6 Wrong display appeared one LCD Caused by electrostatic pulse or power surge screen please turn off and then turn on the machine lt will not affect program running A Please contact the local distributor in time if the above problems can t be solved Ndo drodd vr isal er Gerd zr Wav d9rodd Lr L404 09 8duvodg LUO V asfi er asn 22033 ir ssn 200 2970 agi 209033 vr IHOdMOWE d9104J er asn dzio44 er Ndo delodd ir Wdy 9dUV Og H ANN 92033 9r IHG 92033 Sf NEV d2loss or Siw 20033 er 02 531 202033 en 1 027058 drodd Lr Ndo cd 219709 AgileCycler 2009 09 059 00 L OV OS Ro Foe SE po pos E M COEM LE EE Q od mmm mi OO CE OO OO LL valgo s i oo 55 I xe I TF nnn PP 2y en Gp efe m m c mimi 6 J OO mM 3 M9018 GQNLOH SQ HV Og vauv Og e auvodg c D m 35013 T c 5 m 009 008 HONI8 09 1 vz r m 31 2 13 l FRA Cc Co CC C Ce Ca Ca Ca C C C CL C Cc CC ESS PEED PE OPN ala
16. d touch OK button to add a temperature gradient 6 Save file When editing is completed touch Done to enter Save File screen Figure 13 and touch Browse button to set file saving path Then touch File Name button to name file and finally touch Save button to save file and return File Library screen Figure 14 Save File 12 06 05 17 05 02 File Name cro jud Message Caps Lock Cancel Enter r88 Users USB Flash Browse File Name Save Back Figure 13 Name file and select the path 16 AgileCycler File Library 12 06 05 17 05 02 2 93 Users File name Grad amp 2 Peter Create time 2012 4 26 13 58 2 2 Alex Gr26E td m f Billy E System Preivous runs 6 USB Flash vty LAN users 7 Temp 12 0 C Time Forever New File Print Options Figure 14 New file in the library 3 2 Print file Touch Print button to print selected file The Instrument will connect to Bluetooth printer automatically when the printer is on 3 3 Delete file Touch Options button in the File Library screen Figure 8 to select the file to delete and the query dialog window will pop up when touching Delete button When confirm a deleting touch Yes button Figure 15 To prevent any files being mistakenly deleted only one delete per time is allowed File Library 12 06 05 17 05 02 2 Users File name t3 d Peter Create time 2012 6 5 11 02 p
17. e Step Each File can contain up to 30 Steps Temperature Step contains Temperature Time Gradient Ramp Rate Temperature Increment and Time Extend The maximum cycle in Cycle Step is 99 3 1 Create and edit file In the Home screen Figure 7 touch File button to enter File Library screen Figure 8 File Library 12 06 05 17 05 02 2 Users File name Gr26E 2 Peter Create time 2012 5 7 18 30 5 62 Alex Gr26E td amp Billy El System Preivous runs USB Flash LAN users 7 Temp 12 0 C Time Forever New File Print Options Figure 8 File Library 1 Change Target Temperature and Hold Time From a new file Touch New File button in the File Library screen Fig 8 to create new file Fig 9 Edit File 12 06 05 17 05 02 25 0 Temp 25 0 Increment 0 0 C Cyc Gradient 0 0 C Time Forever Extend 0 s Cyc Ramp Rate 0 0 C s Temp Cycle Delete Options Figure 9 Create a new file Em AgileCycler In the New File screen touch Temp button to add a Temperature Step touch Cycle button to add Cycle Step GOTO step use the pop up numeric keypad to enter a new value for the target temperature or hold time From an existing file Select a file in the File Library screen Fig 8 touch Edit button to modify selected files Edit File 12 06 05 17 05 02 G 0 T 0 X Ten a Se
18. e check form and send a photocopy or via fax back to the distributor after finishing item checking The returned form will be filed into Infinigen Biotech Inc s archives and maintenance record Please store the package and packing materials in a safe place for future uses The above warranty does not extend to goods damaged due to improper package AgileCycler CONTENTS CRAP TER T INSTALLATION S SSNS SASS aa 1 1 The AgileCycler Color Touch Thermal Cycler package includes 1 2 Normal Operating GONGIVIONS aeter eet rov Eb re i ei Do UE EP 1 3 Transportation and StOFage us Serio estie veta ra e bed PUES 1 4 Parts and FUNCTIONS 12 2 2 1 00170 1 5 Mod le changing susene a tm annie ates 4 6 3 69 01016 Shani E thee ore e a 6 7 SPECIFICA UON maai TET 7 GHAPTER2 OPERATION GUIDE 3 892 0t argu Fade FI DURER RE ctr Psi 9 1 Power om Procedure SSR 1 1 1 1 1 1 1 00000 e 9 Ll Ecc T 9 Lob NV AIIM UD a d ora E AA aded orit 10 2 Ment Ecidquieniig dm m nt 11 3 FING haus NE RR MONI E NU T M E 12 3 ANG CCIE Ml SSSR SER Be 12 32a PORE CIS taka eeet DU 17 3 3 Delete Me cscri nnr e 17 DG CODY TE oaa 17 35 Rename Tiler iee pER CRANE RAS CROERR OE KOREA EA RUNS OE 18 FOS RUNTE aA ERR NIME tU M M EE tU NUM 18
19. f a step with each cycle where a positive number increases the temperature and a negative number decreases the temperature Applies only to a temperature step Instructs the thermal cycler to ramp tp the target temperature at the specified ramp rate in that step Instructs the thermal cycler to hold the temperature for the specified amount of time Applies to both temperature and gradient steps Instructs the thermal cycler to extend the hold time with each cycle A positive number increases the hold time and a negative number decreases the hold time Insert a step if a new temperature Cycle GOTO or gradient step is needed Follow these instructions to insert a step to the right of a preexisting step Touch a step to the left where the new step will be inserted 15 AgileCycler Il Touch the Temp button to insert a step or the Cycle button to insert a Cycle GOTO Ill Touch the time or temperature field to edit the parameter in the new step or touch the step or times field to edit the parameter in the new Cycle GOTO 4 Delete a Step To permanently remove a step from file l Select the step to be deleted ll Touch Delete button to delete the selected step 5 Remove or add a temperature gradient Select a step in Edit file screen and touch the Options button to enter Step Options screen Touch Cancel button to remove a temperature gradient or fill the Step Options an
20. hort circuit Hardware problem contact certified mechanic 10 Module sensor 4 open circuit Hardware problem contact certified mechanic 11 Radiator sensor short circuit Hardware problem contact certified mechanic 12 Radiator sensor open circuit Hardware problem contact certified mechanic 13 lid sensor short circuit Hardware problem contact certified mechanic 14 lid sensor open circuit Hardware problem contact certified mechanic 15 Hardware problem contact certified mechanic 204 AgileCycler 16 Block temperature too high Air flow vent is blocked or circuit problem or need repair 17 Block temperature too low Environmental temperature is too low or circuit problem or need repair 18 Heat Sink temperature too high Air flow vent is blocked or fan problem or need repair 19 Heat Sink temperature too low Environmental temperature is too low or circuit problem or need repair 20 Hot Lid temperature too high circuit problem or need repair 5 Cause of the abnormal phenomenon amp solution No Description of problem Cause and solution 1 No display after booting Check whether the plug is inserted correctly and check the power output Please turn off the machine and disconnect the plug to check the fuse 2 After power on the machine start The power was shut down before the previous running the program program is completed The fan runs fast some time slows Normal The fan is for cooling the heating pump down some time not for rea
21. is better to apply low voltage fluctuation power sources otherwise power source regulator should be applied 2 Itis strictly prohibited to terminate a running experiment by cutting off the power Doing so will be very harmful to the machine 3 2 LCD touch screen Do not UV sterilize the LCD screen to prevent causing any damage Avoid any bump or scratch to the LCD screen while using 2065 AgileCycler 3 3 Notice on cleaning Please avoid any liquid getting inside the machine while cleaning reaction block Due to the possible usage of radioactive substance during experiment please carefully handle cleaning Please do not operate and use the machine in moisture or hot environment A Attention Please do read the Notices carefully to prevent causing any damage to the machine 4 Error message amp solution No Cause and solution 1 Null character can not be used for file name 2 Same file name Please re name Do not support multiple files with same file 3 Module sensor 1 short circuit Hardware problem contact certified mechanic 4 Module sensor 1 open circuit Hardware problem contact certified mechanic 5 Module sensor 2 short circuit Hardware problem contact certified mechanic 6 Module sensor 2 open circuit Hardware problem contact certified mechanic 7 Module sensor 3 short circuit Hardware problem contact certified mechanic 8 Module sensor 3 open circuit Hardware problem contact certified mechanic 9 Module sensor 4 s
22. ranty The Infinigen Biotech Inc is obligated to exchange the Thermal Cycler with any defects result in material and manufacture The Thermal Cycler is also warranted for thirteen months starting from date of ship out Under this warranty The Infinigen Biotech Inc s obligation shall be limited to repair or exchange at the Infinigen Biotech Inc s option of the Thermal Cycler proven to be defective as described herein The buyer is responsible for freight to the maintenance shop designated by the Infinigen Biotech Inc as well as the freight cost for all warranty claims The Infinigen Biotech Inc will cover the transportation expenses of the freight from maintenance shop back to the buyer only applicable to domestic freight After the warranty is due the Infinigen Biotech Inc reserves the right to charge cost for maintenance accordingly 2 Warranty Terms The above warranty is not applicable to defective devices due to improper usage abnormal operating conditions improper application and unauthorized maintenance or alteration The Infinigen Biotech Inc provides no extension warranties other than those described herein Any descriptions in sales promotion shall not create an extension warranty iii AgileCycler Note Check items included in the package in accordance to the checklist at receive Contact the distributor when finding any missing or damaged items in the package Buyers are requested to fill out th
23. re 12 Gradient on Step 3 Table 1 List of parameters for temperature and gradient steps Temperature Temperature in C The target Instructs the thermal cycler to temperature between 0 0 and 100 000 ramp to the target temperature in tenths of a drgree Hold time The hold tine between 1 sec and 59min 59 sec in the format of min sec To enter an infinite hold touch 0 button the symbol infinite will come out Gradient range Lower The lower temperature in the Instructs the thermal cycler to gradient Enter a number between 30 0 ramp to the target temperature and 99 90 in tenths of a degree gradient across the block and hold that temperature gradient m AgileCycler L4O 2200 Upper The upper temperature in the gradient The max temperature is 100 0 C Enter a temperature within 30 0 C of the lower temperature Hold time The hold tine between 1 sec and 59min 59 sec in the format of min sec To enter an infinite hold touch 0 button the symbol infinite will come out A temperature from 9 9 C to 9 9 C per cycle in tenths of a degree A number from 0 1 to 5C per sec A time from 1 sec to 59 min 59 sec A time from 9 min 59 sec to 9min 59 sec per cycle 3 Insert a Step for the specified amount of time Applies only to a temperature step Instructs the thermal cycler to increment change the target temperature o
24. rohibited Any component changing or parameter adjusting inside Thermal Cycler should only be done by certificated professional personnel Do not change any element while the power is still on d A C Power Before turning the power on always check whether power source matches the required voltage 100 240V and power output specification i e 600W e Power Cord Only use the power cord issued by manufacture for Thermal Cycler Don t not attempt to repair any AgileCycler damaged power line a damaged power cord must be replaced The power cord should be kept free from heavy objects during operation The power cord should also kept away from tripping up people f Connection Hold the 3 pin plug and insert thoroughly to power socket to ensure good contact When disconnecting pull the plug not the line g Operating Environment The Thermal Cycler should be placed in a low moisture dust free and good ventilation environment where no caustic gas or powerful magnetic interference exist In addition Thermal Cycler should be kept distant from wet environment such as pools and water pipes Never cover or obstruct the air vents of the Thermal Cycler which are designed for ventilation and to prevent the device s interior from overheating When a single device is running minimum 30cm distance should be kept between air vents and the nearest object When 2 or more devices are running simultaneously a minimum 60 cm distance is needed
25. s or tape to produce consistent and successful reactions Heating the inner lid prevents condensation while applying force seals the reaction to prevent evaporation Warning After a run the heated inner lid can remain hot Use caution when opening and close the lid AgileCycler 5 Module changing Note 1 Make sure the power is off before module changing 2 Maintain module temperature as close as the ambient temperature Step 1 Open PCH lid and remove the 2 screws on the back of PCR by turning counterclockwise Screws Step 2 Extract module by pulling the handle outwards AgileCycler Step 3 Before being completely extracted hold the module to prevent it from falling off Step 5 Push new module inwards completely to insure a fixed connection between the module and machine AgileCycler Step 6 Tighten the 2 screws and close PCR lid after inserting new module 6 Features Each thermal block has 4 independent temperature control sensors and 8 peltier heating units to ensure accurate and uniform temperature across block surface and provide users for replicate previous condition set up 8 TFT color touch screen with graphical display provides easy use for setting up and monitoring The actual and target temperatures can be viewed and displayed graphically in real time 30 thermal cyclers can be simultaneously linked to one PC via LAN which eliminates troubles of setting up and programming each therm
26. tem time when file starts to run Remain Time remaining time for the experiment Lid Temp current hot lid temperature e Step current step e Step Time current step time Block Temp current block temperature 2 Pause Touch Pause button to pause running file and touch Resume button to restore running 3 Stop Touch Stop button to stop a running file 4 Skip Touch Skip button to jump to next step 5 Go to other screen Touch Home button to return Home screen Touch View button to enter the Navigation screen Figure19 Navigation 12 06 05 2 Block Status Idle O B Block Status Invalid File Library Home Figure 18 The navigation screen 4 Setting Touch Setting button in the Home screen to enter the Setting screen Figure 19 19 AgileCycler setting 12 06 05 17 05 02 1 Run Setting 2 System Setting 3 Factory Setting Figure 19 Setting menu 4 1 Run Setting Select the Run Setting button in the Setting screen Figure 19 to enter the Run Setting screen Figure 20 Run Setting 12 06 05 2 1 Hot lid Temp 05 0 2 Hot lid work mode Hot lid off Hot lid on when power on Hot lid on when file running File start when lid Temp attained 3 Lid heater auto shutoff Temp CG 4 Temp Control Block control mode Tube control mode 1 Hot lid Temp G 2 Hot lid work mode Hot lid off Hot
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