xxsealer user manual
Contents
1. Power Inlet On Off Switch and Fuse Holder gt 85 XXSEALER USER MANUAL 1 0 Page 7 The xxsealer unit is designed to be used with a range of sample plates and consumables some of which are listed below Table 3 Plate and Adaptor Block Types Adaptor Block Type Suitable Plate Types BJS xxplate Suitable for 24 54 and 96 well xxplates 96 Well Microtiter Plate 384 Well Microtiter Plate PCR 96 Well Skirted Plate i 0000 000000 7 jf j f 7 j PCR 96 Well Semi skirted Plate PCR 96 Well Un skirted Plate Deepwell Plates Polypropylene Polyethylene or Plate Material Types Polystyrene Other specialist adaptor plates may be available at request Please contact your distributor for details XXSEALER USER MANUAL 1 0 Page 8 Table 4 Recommended Film and Foil Types Clear Polyester Polypropylene Laminate Clear Polymer Thin Clear Polymer Foil Polypropylene Laminate Foil Laminate Foil Note The only film approved for use with xxplates i Muia es is the B i sealing film Part number HS 3001 01 1 P se IN L 93936818954 xxpress HEAT SEALING SHEETS HEAT SEALING SHEETS Quantity 10 Catalogue HS 3001 01 1 XXSEALER USER MANUAL 1 0 4 Technical Specification Model Type Model Name Model No Physical Unit Properties Dimensions W x D x H Weight without adaptor Mains Supply Power Cord Rating Inlet Module Type Supply Voltage Selections S2. ccceecceceeeceeceeeeeceeeeeceeeeseeeesseeeeeseaeeessaeeeesaaes 9 5 Safety Precautions and Limitations of US ccccceccceececeeeeeeeeeeeeees 10 6 TANG NNSA ATION erospen EEE E E E EE EEE 14 7 UA ODS aO ern E EE 16 Fat Us rDisplay and COniOlS srera irei E n A 16 7 2 Operating the Controls of the xxS QleP ccccccceececeeeeeeeeeeseeeeeseeeeeeas 17 7 3 Performing an xxplate Sealing Operation ccccccceesseeeeeeseeeeeeeeeeees 18 7 4 Switching Off the Audible Alert cccccccccccseeeceeeeeeeeeeeeeeeseeeeseeeeesaes 20 8 Maintenance and Servicing cccccseeccecceseceeeeeseeeseeeceeseeeeeeeseeeeeessaeees 21 ST RopacnNg INE UNIT ast Lo oe eee 21 8 2 Over Temperature Safety Cut Out ccccccccccsseceeeeeeeeseeeeeeeeeesseeeenens 22 8 3 Routine Cleaning and INSPECTION cccccceecceceeececeeeeeeeeeeseeeeessaeeeeeas 22 8 4 Decontamination Procedure cccccccsscccssseccseeeceueecseessueesseeeessueessaes 23 8 5 Transportation And StOrage ccccccccccssscecseeceeceeeeeseeseeseeeesseeeeeseeeeeeas 23 9 VY Ai anhy AG ROUN eera E E E 25 10 Product DiISposSal saser cers tere ceep eserecsepsennconset vossnonciecteon eens EEEN EEEE ETE 26 Tables Table 1 Advisory Symbol Meaningg c ccccccseeeeeeeeeeceeeeeeseeeeesaeeees 5 WONG 2 Unit Features screens serene page ennonn nN ese ees A NE aaa 6 Table 3 Plate and Adaptor Block Types cccccceeceesee
3. the unit Improper use of this unit or its accessories may impair their functionality and invalidate the manufacturer s warranty Unit Handling Precautions Care should be taken not to drop the unit or subject it to rough physical handling both during normal use and during transportation and storage The unit should be held by the handle when being lifted or moved Do not lift the unit by any other part of the casework Care should be taken when lifting the unit due to its weight Care should be taken not to knock the LED display Do not use excessive force when pressing the control buttons or operating the seal activation handle XXSEALER USER MANUAL 1 0 Page 11 Unit Installation and Operating Environment The xxsealer unit is designed for indoor laboratory use only The acceptable operating temperature range is 18 C to 30 C with a relative humidity of 20 to 80 non condensing at a maximum altitude of 2200m above sea level lf the unit is stored in conditions outside of these ranges it must be left to stand unpowered until it has acclimatised to within these environmental limits before being powered Use only the AC mains power cord provided with the unit or as specified Section 4 The unit must be connected to a suitably earthed mains supply with appropriate earth leakage and over current protection Always ensure that the mains power connector is securely inserted into the rear of the unit and any
4. 1 Switch off the unit and disconnect the power before performing any inspection checks or cleaning 2 Before cleaning always inspect the unit casework heater plate and moving parts for any signs of wear damage cracks or other defects 3 Clean the casework using a damp cloth soaked with a disinfectant solution Such as Virkon whilst wearing suitable PPE 4 Carefully clean the heater plate surface to remove any debris or sealing material 5 Remove any debris or fluff from around or between the moving parts of the handle mechanism and sliding plate carrier 6 Clean the display and buttons taking care to avoid over wetting 7 Check that the ventilation holes on the rear of the unit are clear of dust and fluff build up 8 Check and clean the adaptor blocks JN After cleaning ensure that the unit is thoroughly dry especially around the mains power inlet before reconnecting the power DANGER cord and switching the unit on XXSEALER USER MANUAL 1 0 Page 23 8 4 Decontamination Procedure The unit and accessories should be decontaminated using the following procedure before being stored or transported Decontamination Procedure Thoroughly clean all outside surfaces of the product including any accessories power cords manuals packaging etc with a damp cloth soaked with suitable disinfectant solution Such as Virkon 8 5 Transportation and Storage The xxsealer unit and its accessories should be
5. EALER USER MANUAL 1 0 Page 26 10 Product Disposal At end of life this product must be disposed of in accordance with your local authority regulations for the disposal of potentially hazardous waste and electronic equipment The unit and its accessories should be decontaminated using the procedure detailed in Section 8 4 before disposal or shipping Do not dispose of this product into unsorted municipal waste or public landfill Please contact your distributor or BJS Biotechnologies at the address on Page 2 of this manual for details of how to correctly dispose of this product XXSEALER USER MANUAL 1 0
6. LED XXSEALER USER MANUAL 1 0 Page 17 7 2 Operating the Controls of the xxsealer When the unit is switched on it recalls the last sealing operation settings and the displays the sealing temperature or time depending on the display mode and automatically restores the heater on off state Pressing the MODE button toggles the LED display between the plate heater Temperature set point in degrees centigrade and the seal Timer setting in seconds The sealing temperature is set by pressing the MODE buiton to illuminate the Temperature LED C then using the UP and DOWN buttons to set the temperature in the range 125 to 200 C in 1 C steps Likewise the sealing time is set by pressing the MODE button to illuminate the Timer LED then using the UP and DOWN buttons to set the time in the range 1 to 9 seconds in 0 5 second steps Pressing the HEATER button toggles the plate heater between on and off When the Heater LED is flashing the heater is on and the plate is warming up which will normally take about 10 minutes Once the Heater is on the LED display shows the current temperature or sealing time set point depending on the mode When the heater plate is up to temperature within 2 C of the set point the Heater LED will remain constantly on and the xxsealer is ready to begin sealing The operator may set a precise temperature and seal time for the sealing operation Sealing is initiated by pulling the handle d
7. ack on again using the following procedure 1 If necessary press the MODE buiton to select the Temperature LED setting mode 2 Next simultaneously press both the HEATER and MODE buttons together 3 The display should show 01 or OO If it does not repeat this procedure from step 1 above 4 Now press the DOWN buiton to turn the alert off display shows 00 or the UP button to turn the alert back on display shows 01 5 Finally press the MODE button to store the new setting XXSEALER USER MANUAL 1 0 Page 21 8 Maintenance and Servicing Although the xxsealer unit does not require any scheduled maintenance or servicing the operator should regularly clean and inspect the unit for any detects as described in Section 8 3 below Please observe and comply with all of the Unit Maintenance and Serviceability precautions listed in Section 8 Never removed the unit casework There are no user or operator serviceable parts inside the unit Always switch off and unplug the unit before performing any cleaning or disinfecting tasks For technical and service related enquiries please contact your distributor or BJS Bio at the address given on Page 2 of this manual 8 1 Replacing the Unit Fuse The unit fuse should only be replaced by a suitably qualified technician A DANGER The unit fuse will only blow as a result of an internal unit fault or if the voltage selector switch has been inc
8. anings Indicates a Risk of Electric Shock which could if not avoided result in serve injury or death Indicates a Burn Hazard which could if not avoided result in serve injury or death Indicates a Risk of Explosion which could if not avoided result in serve injury or death Indicates a hazardous situation which could if not avoided result in serve injury or death or severely damage the unit WARNING Indicates a hazardous situation which could if not avoided result in minor or moderate injury or degrade or impair the functionality of the unit CAUTION Indicates a Risk of Crush hazard due to moving parts which could if not avoided result in minor or moderate Injury CAUTION Jury i Advisory note or other useful information gt NN Refer to Section NN for more details XXSEALER USER MANUAL 1 0 Page 6 3 Unit Description The xxsealer provides a safe and controlled method for sealing plastic sample plates to protect the samples from evaporation and contamination during the heating and cooling of a PCR process The sealer is adjustable for both the sealing time and temperature and gives a visual countdown display during the sealing process for consistent results The unit has the following external features Table 2 Unit Features Seal Activation Handle 7 2 User Interface Display and Control Buttons gt 7 1 Heater Plate Internal gt 7 1 Adaptor Block Carrier gt Table 3
9. eeeeeeeeeeeeseeeeens 7 Table 4 Recommended Film and Foil Types ccccceseeeeeeeeeeeeeeees 8 Table 5 Mains Voltage Selection ccccccccceecceceeeeeeeeeeesaeeeessaeeeeeaees 14 Table 6 User Buttons and Status LEDS ccecccceceeeeeeeeeeeeeeeees 16 XXSEALER USER MANUAL 1 0 Page 4 1 Glossary of Terms and Abbreviations ANSI BJS Bio Deepwell Plate DWP EMC Microtitre Plate MTP PCR PPE SBS Semi skirted PCR Plate Skirted PCR Plate Un skirted PCR Plate Well xxplate XXSEALER USER MANUAL 1 0 American National Standards Institute BJS Biotechnologies Limited UK Company Number 02959160 Plate with an SBS footprint featuring 48 96 or 384 wells with a larger volume than microplates Deepwell plate Electro Magnetic Compatibility Plate with an SBS footprint featuring 24 48 96 or 384 wells Microtitre plate Polymerase Chain Reaction Personal Protective Equipment society for Bio molecular Screening PCR plate with an outer surrounding half edge PCR plate with an outer surrounding edge PCR plate without an outer surrounding edge A single sample cavity in a Microtitre plate PCR plate or Deepwell plate BJS Bio PCR test plate for the xxpress real time thermal cycler Available in 24 54 and 96 well formats Page 5 2 Symbols Used in this Instruction Manual The following advisory symbols are used in this manual Table 1 Advisory Symbol Me
10. excess power cord does not pose a potential trip or pull hazard WARNING Do not operate the unit in any area which is or has been or is thought to have been exposed to explosive or flammable gases vapours or liquids The unit must be installed and operated on a solid stable and level working surface ensuring that the ventilation holes at the rear of the unit are not obstructed WARNING XXSEALER USER MANUAL 1 0 Page 12 General Operating Precautions Ensure that the power is switched off at both the AC mains supply outlet and at the back of the unit before inserting or removing the mains power cord The heater plate can reach temperatures of 200 C and will remain hot for a considerable time after being turned off Extreme care must be taken not to touch the heater plate as it will cause a serve burn injury The unit is intended for use with plates containing biological samples only Never use the unit to seal any explosive volatile or highly reactive substances or chemicals There is a possible finger crush hazard due to the moving parts of the handle and plate carrier CAUTION Care should be taken when operating the handle XXSEALER USER MANUAL 1 0 Page 13 Unit Maintenance and Serviceability There are no user or operator serviceable parts inside the unit Do not remove the unit casework Removal of the unit s casework will void the manufacturer s warranty and may expose the user to a Risk o
11. f Electric Shock resulting in serious injury or death Once installed the externally accessible unit fuse will only blow under a fault condition This fuse should only be changed after the unit has been thoroughly inspected by a qualified engineer See Section 8 1 for details Always switch off the unit and disconnect the power cord before performing any cleaning or decontamination procedure If liquid is spilt into or over the unit switch off and disconnect the power from the AC mains outlet before attempting to deal with the spillage Ensure that the heater plate has cooled down to room temperature before performing any cleaning operation and before moving or storing the unit The use of harsh chemicals and cleaning agents may damage the unit and degrade its performance Always follow the cleaning and decontamination procedures CAUTION specified in Sections 8 3 and 8 4 of this instruction manual Do not autoclave any part of the unit or its accessories CAUTION XXSEALER USER MANUAL 1 0 Page 14 6 Unit Installation Before installing the xxsealer unit please check that the delivery is complete and that the unit and any accessory parts are intact and free from any signs of transportation damage Also ensure that all external and internal packaging has been removed from the unit before installation Please retain all packaging for future transportation and storage of the unit and its accessories The xxseale
12. orrectly set see Section 5 This fuse should only be changed after the unit has been thoroughly inspected and must be replaced with the exact type specified in Section 4 Thoroughly inspect the unit for any signs of damage loose components or liquid spillage or ingress If in doubt please contact BJS Bio on the number given on Page 2 of this manual Z N DANGER The fuse holder is removed by disconnecting the mains cord and then using a small flat bladed screwdriver to carefully pull open the fuse holder see Section 5 and remove the old fuse After replacing the fuse with the corrected rated one for the operating voltage being used see Table 5 push the fuse holder firmly back into the inlet module The unit must be electrically safety tested for excess leakage current before being repowered from the mains supply XXSEALER USER MANUAL 1 0 Page 22 8 2 Over Temperature Safety Cut out The unit is fitted with a non resettable thermal fuse which blows if the heater plate temperature exceeds 235 C In the unlikely event of a fault condition this fuse will permanently disable the heater to protect the user and unit from injury and damage If this occurs the heater plate will remain cold and the xxsealer will need to be returned for repair 8 3 Routine Cleaning and Inspection The unit casework should be cleaned and inspected at regulator internals and whenever contamination or spillage occurs as follows
13. own which raises the sample plate into contact with the heater plate The unit detects when the sample plate is correctly positioned and starts a countdown timer When the display reads zero the operator moves the handle back to its upmost position which moves the sample plate away from the heater plate The sample plate is then manually removed for storage or further processing XXSEALER USER MANUAL 1 0 Page 18 7 3 Performing an xxplate Sealing Operation A typical operating sequence to seal an xxplate is 1 Switch the unit on and set the sealing time to 1 5 seconds and the heater plate set point temperature to 170 C using the method described above These are the recommended setting for the xxplate film 2 If necessary press the HEATER button to turn the heater on 3 Allow sufficient time for the unit to reach temperature the Heater LED will stop flashing and remain constantly on 4 Fit the BJS xxplate Adapter Block into the PCR 96 Well Skirted Plate Adapter Block both provided with the xxsealer and locate in the Adapter Block Carrier 5 Load the xxplate onto the xxplate Adapter Block locating it onto the sprung pins It should sit flat on the adapter block And add the sealing film on top the correct way up Care should be taken not to touch the surface of the heater plate whilst loading the sample plate WARNING XXSEALER USER MANUAL 1 0 Page 19 6 Grasp the handle with one or two hands a
14. r unit should be installed in a location which meets the following requirements e Safe and suitable operating environment see Section 5 e Solid stable level working surface e Atleast 10cm clearance around the unit to adjacent objects and walls e Earthed AC mains power connection see Section 4 Please also observe and abide by the Unit Installation and Operating Environment safety precautions and preconditions listed in Section 5 WARNING It is important the check that the Mains Voltage Selector switch on the rear of the unit has been set to the correct position for the desired operating voltage and the correct value of fuse has been fitted see Table 5 Table 5 Mains Voltage Selection Operating Voltage 110 to 120 VAC 220 to 230 VAC Voltage Selection 115 230 see Section 3 for switch location on unit rear panel Fuse Rating 3 15AH 1 6AH XXSEALER USER MANUAL 1 0 Page 15 Ensure that the correct fuse type has been fitted in the mains inlet fuse holder for the desired voltage selector switch value See Table 5 for details WARNING 1 Remove the AC plug from the power inlet module 2 Use a small flat bladed screwdriver to carefully pull out the fuse holder 3 Fit the correct fuse in the far position only 4 Push the holder back into the inlet mode Install and test the xxsealer unit using the following procedure 1 Place the unit on the suitably selected working surface as
15. s appropriate and pull the handle forward and down to raise the plate carrier until the sample plate contacts the heater plate 7 When correct sealing pressure is achieved an audible alert will sound if enabled see Section 7 4 and the timer will start counting down from the set value When zero seconds is reach the display will flash and the audible alert sound f Do not to apply more force to the handle than is necessary to operate caution the start switch 8 Immediately return the handle to its upmost position to retract the sealed sample plate from the heater plate Failure to release the handle when sealing is complete could degrade caution the sample plate and seal quality 9 Remove the sealed plate from the unit and review the seal integrity The sample plate and film foil may A remain hot for several seconds after being removed from the unit WARNING For optimum performance the sealing temperature and or time may need to be adjusted to best suit the sample plate and film foil types being sealed Various types of plastic sample plate may be loaded onto a plate carrier and thermal sealed using a suitable foil or clear film See Section 3 for details XXSEALER USER MANUAL 1 0 Page 20 7 4 Switching Off the Audible Alert The unit is fitted with an audible alert which sounds at the beginning and end of each sealing operation If desired this alert can be turned off or b
16. specified above ensuring that the ventilation holes on the rear of the unit are not obstructed 2 Connect the unit to the AC mains power outlet using the mains power cord supplied or as specified in Section 4 3 Switch the mains power on at supply outlet first and then switch the unit on using the power switch located at the rear of the unit 4 Check that the unit is stable and safe by performing a trial sealing operation as described in Section 7 2 lf the unit has been stored in a cool environment it must be left to stand unplugged until it has acclimatised to the new room temperature before being powered DANGER XXSEALER USER MANUAL 1 0 Page 16 7 Unit Operation Please ensure that you have read and fully understood all of the Safety Precautions and Limitations of Use listed in Section 5 before attempting to operate the xxsealer unit WARNING 7 1 User Display and Controls The unit s user interface consists of a large 3 digit LED display four control buttons and three status indicator LEDs The LED display shows the current temperature or sealing time set point depending on the mode The button and LED functions are listed in Table 6 Table 6 User Buttons and Status LEDs O Off Heater Turned Off Heater Status LED Flashing Warming Up HEATER On Off Button C On Unit Ready For Use DOWN Button O UP Button MODE Select Button C Temperature Mode LED Timer Mode
17. t when purchased new and installed and operated in accordance with the instructions of this manual to be free from defects in materials and workmanship and will repair or replace at their discretion any unit or accessory which exhibits such defects In no event will BUS Biotechnologies be liable for any indirect incidental or consequential damages resulting from any defect or warranty claim Unspecified use or unauthorised modification of any part of the xxsealer unit or its accessories or the use or attachment of any adaptor or peripheral not supplied specified or sanctioned by BJS Biotechnologies will invalidate this warranty This warranty is provided to the original purchaser of the product for one year from the date of purchase Under the terms of this warranty the product must be returned in its Original packaging transportation prepaid by the sender with a copy of the Proof of Purchase and a detailed description of the problem The product must be decontaminated using the procedure detailed in Section 8 4 and a Certificate of Decontamination supplied with any return If the product is considered too hazardous to be shipped please contact BUS Biotechnologies WARNING onthe number given on Page 2 of this manual for further instructions Please contact your distributor or BUS Biotechnologies on the number given on Page 2 of this manual to assess the defect and arrange the return the product if necessary XXS
18. thoroughly decontaminated using the procedure detailed in Section 8 4 before being placed in its original packaging for transportation or storage Refer to Section 4 for the acceptable range of Storage and Transportation environmental conditions Always ensure that the unit and accessories are completely WARNING dry and free of any condensation before being packed XXSEALER USER MANUAL 1 0 Page 24 Certificate of Decontamination We respect the health and safety of our clients and employees and request that any products or accessories being returned are decontaminated in accordance with the procedure below 1 Decontamination Procedure Thoroughly clean all outside surfaces of the product including any accessories power cords manuals packaging etc with a damp cloth soaked with suitable disinfectant solution Such as Virkon Allow to dry fully before packing 2 Decontamination Declaration Company Name Address Product Code xxsealer 953 A VTS Serial Number Reason For Return Where Product Used Please tick the appropriate option s below O certify that have decontaminated the product as per the above procedure Decontaminant Used O certify that the product has not been exposed to any chemical or biological materials Title Name Signature Date Telephone Email XXSEALER USER MANUAL 1 0 Page 25 9 Warranty and Returns BJS Biotechnologies Ltd warrants the xxsealer produc
19. upply Frequency Range Power Consumption Fuse Ratings and Sizes Operating Environment Temperature Range Relative Humidity Range Maximum Operating Altitude Storage and Transportation Temperature Range Relative Humidity Range Sealing Parameters Temperature Range Timer Range Audible Alert XXSEALER USER MANUAL 1 0 Page 9 xxsealer VTS 953 A 220 mm x 325 mm x 425 mm 7 2 kg IEC C13 3 Core 5A min IEC C14 DPST Single Fuse 110 to 120 VAC 10 220 to 230 VAC 10 50 to 60 Hz 5 350 W max T3 15AH 250V 20x5mm T1 6AH 250V 20x5mm 18 to 30 C 20 to 80 non condensing 2200 m above sea level 10 to 50 C 20 to 95 non condensing 125 to 200 C in 1 C steps 1 0 to 9 0 seconds in 0 5 second steps On or Off Page 10 5 Safety Precautions and Limitations of Use It is essential that all users of this equipment have fully read and understood the following safety precautions and limitations of use before installing or operating the xxsealer unit A WARNING A WARNING A CAUTION A WARNING A CAUTION IMPORTANT The protection provided by this equipment may be impaired if it is not used in a manner described in this manual It is essential that the user of this equipment is aware of the potential hazards associated with the unit and its accessories All operators should be familiar with the safety precautions and warnings given in these instructions before attempting to operate
20. xxsealer PCR plate heat sealer with programmable temperature and dwell xxsealer User Manual BJS BIOTECHNOLOGIES A LIFESCIENCE COMPANY This Manual Copyright 2014 BJS Biotechnologies Page 2 All rights reserved No part of this manual may be reproduced stored in a retrieval system or transmitted by any means or in any form without the prior permission of BUS Biotechnologies Ltd BJS Bio The information contained in this manual is subject to change without notice BUS Bio assumes no responsibility for any errors that may appear in this or related documentation Document XP 5003 01 1 Revision 1 0 Jan 2014 xxpress xxplates and xxsealer are the brand names for BJS Biotechnologies own range of PCR thermal cycler products and accessories For further details please visit our web site www xxpressPCR com All sales and technical enquiries should be addressed to BJS Biotechnologies Ltd 65 Bideford Avenue Perivale Greenford Middlesex UB6 7PP United Kingdom 44 0 203 021 3750 sales xxpressPCR com or support xxpressPCR com XXSEALER USER MANUAL 1 0 Page 3 Contents 1 Glossary of Terms and Abbreviations ccccccccseeeeseeeeceeeeseeeeeaeeeeaees 4 2 symbols Used in this Instruction Manual ccceecseeeeeeeeeeeeeeeeeeeeeees 5 3 Unit Description 0 0 0 ccc cecccceececeeeceeeeceeeeceeeeeeeeeeeeseueesaeeesaeesaueesseesaneeseneenes 6 4 Technical Specification
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