USER MANUAL
Contents
1. The link opens the Welcome page with the details as given below EXPAND TB ExPanding Access to New Diagnostics for TuBerculosis Ston Partnership GE FIND BS gl GLOBAL DRUG toni World H ltt E i FA LIT ic Te Moly Orga ni ti Bocauee Ciagrresis mallets a gt User Login Forgot Password Create Account EXPAND TB Budgeting Application Tool Account Creation When accessing the Budgeting tool for the first time you must create an account to access it The account creation creates a unique profile that keeps track of all the account activity The following are the steps to follow when opening an account e Click on Create Account on the user login home page e Fill in all the details on the create new account page EXPAND TB ExP nrdrea 22888 iS Create New Account Full Name Your Email Country Password Confirm Password SUBMIT LOGIN HERE e Once filled and submitted you will receive an email to the email that you supplied confirming your registration EXPAND TB Budgeting Application Tool System Login After you have successfully created an account you can now access the system by using your Email Address and Password created above EXPAND TB EXPanding Access to New Diagnostics for TuBerculosis 6 Fl N DES g GLOBAL DRUG DRUG World Health Toler To WN Organization beocau ciagiresis2. 5 5ml aliquots of Solution You need 10 ml of Solution per batch of 28 tubes 200ml of media containg drug at 7ml per tube 12 77mg is needed to prepare 25ml of Solution One aliquot of about 12ml DST laboratory chemicals 5g Dihydro streptomycin Total amount 226 Cost for First line drugs 18 8333 UNIT COST UNIT PACK QUANTITY OF PACKS US CALCULATION DETAILS You need 1 mi of Solution per 42 tubes DST laboratory chemicals Ofloxacin Ciprofloxacin 300ml of media containg drug at 7ml per 19 tube 24mg is needed to prepare 10ml of Solution Solution needs to be prepare fresh You need 2 5 ml of Solution per 28 tubes DST laboratory chemicals Protionamid 4 n Propyl 200ml of media containg drug at 7ml per 4 Heptanol 99 2 g tube 41 2mg is needed to prepare 100ml of Solution Rifampicin Solution needs to be You need 2 ml of Solution per 28 tubes 200ml of media containg drug at 7ml per DST laboratory chemicals Kanamycin 1 g tube 39 5mg is needed to prepare 10ml of Solution 1 1ml aliquots of Solution should be You need 2 ml of Solution per 28 tubes 200ml of media containg drug at 7ml per DST laboratory chemicals capreomycin 1 g tube 62 5mg is needed to prepare 10ml of Solution 1 1 aliquots of Solution should be Total amount Cost for Second line drugs The summary section shown below displays the sum total for the Solid Culture Cost of Processing cost
3. pack of 4500 Sterile indicator tape autoclave Sterile indicator tape hot air oven Tissue pulp Tube brush Disinfectant for BSCs surface Disinfectant for floors Disinfectant for hands 11 Ethanol lsopropanol SL Phenol 1Kg Liquid soap spray head Cost for CLEANING SUMMARY Cost for BIOSAFETY 7 051 42 Costfor CLEANING 4 104 74 Total Cost 11 956 13 EXPAND TB Budgeting Application Tool QUANTITY OF PACKS 2 11 2 4 2 9 18 9 52 11 11 11 2 1 QUANTITY OF PACKS UNIT COST USS 32 16 UNIT COST US 86 8 99 14 342 19 3 14 32 45 37 24 CALCULATION DETAILS 1 per day per staff 4 per day per staff 0 33 per week per staff 0 66 per week per staff 0 33 per week per staff 10 per staff per day 10 per staff per day 10 per staff per day 2 per staff per week 1 per BSC per week 1 per BSC per week 1 per BSC per week 2 per year 1 per 3 years CALCULATION DETAILS 1 roll per 6 months 1 roll per 2 months 3 per day for changing room 1per BSC 2 per day per BSC 3 per day 1 per 2 months 2 per quarter 2 per quarter 2 per BSC per day for culture 1 per 500 cultures 1 bottle per quarter per BSC 1 per 2 months 2 per month 2 bottle per quarter per BSC 259 per day 3 per month 4 per year per bsc 21 Maintenance and Repair To calculate the Costs for equipment service amp maintenance contracts the system provides a list of editable list of commonly used equipment in the laboratory with a tab Ma
4. Biosafety and Cleaning Biosafety Cleaning Sub Total Maintenance and Repair Costs for Equipment service amp maintenance contracts Costs for equipment repair w out service contracts Sub Total Fuel generator Electricity Request forms Registers Telephone amp internet Dest H20 Costs USD 3 034 21 449 73 287 06 273 73 1 773 06 365 977 99 2 060 60 373 856 38 Costs USD 7 851 42 4 104 71 11 956 13 Costs USD Costs USD Number of tests 10362 Cost per test The tool has an option for downloading the Budget summary in PDF format To do so go to the Summary page and Click on Download Budget Summary You can also export the data of the Budget summary into Excel Dashboard Log Out Country SWITZERLAND Reset Dashboard Country SWITZERLAND Download Budget Summary Export to Excel Period of Budget 0 01 Months EXPAND TB Budgeting Application Tool 27
5. 0 Laboratory technicians microscop 0 Laboratory Clerk 0 Nurse assistant Cleaner 0 Microscopy supervisor 0 Total for incentives and salaries 0 00 Create New Section Total Cost for Human Resource 0 00 Quality Assurance Quality Assurance section calculates the total cost required for the set Supervisions as shown below The default value for all the sections is Zero Quality Assurance Items Supervision visits for AFB laboratories periphery Supervision visits for C DST laboratories by NRL Name Value Name Value Number of supervision visits in one year per facility i No tems Number of facilities to be supervised l Supervision visits for NRL s by SRL Length of supervision visi average number of days Number of national expert facilitators per supervision 8 eae visit Number of support staff e g driver per supervision visit No items Cost for fuel and lubricants typical per wisit Re checking samples NRL gt SRL Costs for Supervision Total Cost for fuel and lubricants Name Value Total per diem national expert facilitators No items Total per diem for support staff Total cost of hotel charges for staff Panel testing SRL NRL Other costs Mame Walue Total costs for Supervision No items Total Quality Assurance Cost 0 00 Create New Section EXPAND TB Budgeting Application Tool 24 Operations Operations section gives an itemized list of the operationa
6. Precision balance 0 00 Twincubator LP A Travel accomodation technician 0 00 Binocular light microscope 0 00 Binocular fluorescence microscope mercury lamp 0 00 Binocular LED fluorescence microscope Primo Star iLED UPS Module to convert bright field microscope in LED transmit Module to convert bright field microscope in LED re emitte Incubator Centrifuge for standard reaction tubes Autoclave f no service contract Water distiller Separate water supply for deionised water with conductiv Aqua purificator cabinet for two de mineralising cartridges Compressor for autoclave pH meter 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 D D D D Ss EA O O E 5 Es 5 Water bath capacity approximately 40 L Maintenance and Repair tems Manage Items Add Maintenance amp Repair 2 Export daw Print SectionID Equipment Actions i Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipmen
7. left navigation On the microscopy section the total cost for per smear total amount microscopy can be calculated by specifying the number of smears for the period specified on the general settings The system automatically computes and displays an itemized list of the required quantities to conduct the specified number of tests for the given period Microscopy items Parameters Name Number of smears from culture Total number of smears for the period Number of smears for the period EXPAND TB Budgeting Application Tool 10 Calculation formula 1 Number of smears from culture Liquid Culture and DST Number of total test for the period including contamination and repeat Processing and Solid Culture Average volume per specimen in ml to be decontaminated Processing and Solid Culture of MTB positive 2 Total Amount Microscopy Total amount Reagents Item 1 Total amount Reagents Item 2 UNIT COST US Basic fuchsine 100g bottle 100 63 Carbol Fuchsin 5ml per slide includes 10 ITEMS UNIT PACK QUANTITY OF PACKS CALCULATION DETAILS Methylene blue 100g bottle 100 109 Methylene Blue 5ml per slide includes 10 Phenol crystals colourless 5kg 96 includes 10 Ethanol 96 2 5L bottle for stain solutions 7 includes 10 Ethanol 96 2 5L bottle for decolourization 7 Decolourization per slide includes 10 Hydrochloric acid 2 5L bottle 27 includes 10 Total amount C
8. matiess User Login Email Address Password Forgot Password Create Account EXPAND TB Budgeting Application Tool Password Reset In case you forgot your password Click on Forgot password enter your email address and click on recover A password reset link will be sent your email address EXPAND TB EXPanding Access to New Diagnostics for TuBerculosis Recover Password EXPAND TB EXPanding Access to New Diagnostics for TuBerculosis Email Sent An Email Has been sent To You Click the Link to Change Password LOGIN gt Enter a new password and Log in to access your account EXPAND TB EXPanding Accoss lo Now Diagnostica for TuBoroulosia Recover Password Enter Password Confirm P Password EXPAND TB Budgeting Application Tool Account Setup EXPAND TB EXPanding Access to New Diagnostics for TuBerculos Budget Summary General Settings gt Microscopy Processing and Solid Culture LPA Line Probe Assay gt Xpert MTB RIF Liquid Culture and DST Biosafety and Cleaning gt Maintenance and Repair gt Human Resource QA Quality Assurance Operations Draft Report copyright92015 EXPAND TB Budgeting Software The Left Navigation provides different sections of the tool which displays information depending on the selected link General Settings The general settings section controls different parameters to be applied to the budg
9. 0 per day of final solution T2 gr per liter 40 per day 1 Liter per week 16 Xpert MTB RIF Xpert MTB RIF section can be accessed by clicking on Xpert MTB RIF on the left navigation Xpert MTB RIF Items Parameters Number of tests for repetition 1 0E 10 Number of total test for the period including repeated tests 0 0001000001 Number of Xpert MTB RIF tests for the period 0 0001 Expected unsucessful test rate 0 0001 _ SAVE Parameters section provides for the number of Xpert MTB RIF tests for the period and expected unsuccessful test rate when supplied calculates the list below e Number of tests for repetition Number of total test for the period including repeated tests ltems required for the test Name Quantity Unit Cost and Calculation Details and e Summation Total Amount to run the tests and the cost per test Parameters Number of tests for repetition Number of total test for the period including repeated tests 4 204 Number of Xpert MTB RIF tests for the period 200 Expected unsucessful test rate 2 SAVE UNIT PACK QUANTITY OF PACKS UNIT COST US CALCULATION DETAILS Xpert MTB RIF cartridges 10 21 100 Use either PP tubes or normal containers and set the PP tubes for centrifuge sterile 50 ml 500 0 109 other to 0 Includes 10 Sputum containers pack 100 100 a 11 25 Includes 10 Total amount Cost for xpertmtb SUMMARY Total amount 2 064 Cost for one Xpe
10. 25 Cryo vial sterile with cap 2 mi 1000 1 324 Cryo tags sized to fit for use on cryo tubes rolls of 1000 1 1 35 Deep freeze storage box with lid for 1 5 2 ml cryovials autoclavable PP 1 4 3 Petri dishes plastic 480 1 37 Rapid test for Detection of MPT 64 Antigen 25 52 35 Brain Heart Infusion agar 05 1 114 Plastic foil 1 2 55 Total amount Cost for Liquid Cultures Drug Susceptibility Testing ITEMS UNIT PACK QUANTITY OF PACKS UNIT COST US BUDGET BACTEC MGIT 960 SIRE kit One kit is sufficient for 40 test 40 23 73 1670 BBL MGIT Tubes for use in Bactec MGIT 960 7ml 100 tubes pkg 100 46 195 8958 TIPS PP 20 200 71 sterile autoclavable with Filter 960 2 93 174 TIPS PP 100 1000 21 sterile autoclavable with Filter 960 62 115 Dispenser tips Universal 10ml sterile 100 4 107 338 BACTEC MGIT PZA Kit 50 19 91 1665 BACTE MGI PZA Tubes 25 25 74 54 3936 TIPS PP 20 200 71 sterile autoclavable with Filter 960 93 94 TIPS PP 100 1000 21 sterile autoclavable with Filter 960 62 62 Total amount 17 008 37 Cost for Drug Susceptibility Testing 1 82 SUMMARY Cost for Liquid Cultures Cost for Drug Susceptibility Testing TOTAL COST LIQUID CULTURE amp DST 14 573 27 17 008 37 31 581 64 EXPAND TB Budgeting Application Tool BUDGET 7986 2908 144 885 454 85 10 1813 114 109 14 573 27 1 56 CALCULATION DETAILS 1 tube per test 1 tube per test 10 exchange per year Same than processing 0 if processing 1 tub
11. 70 4 45 41 403 4 34 38 103 4 3287 61 3 135 23 16 87 15 48 1 84 22 13 18 138 38 4 06 24 18 16 11 68 BUDGET 2517840 1022 4 1024 95 403 4 190 15 110 53 192 94 214 73 475 49 475 01 139 29 24228 73 08 91 24 45 85 121 38 365 977 99 3 26 CALCULATION DETAILS Volume needed is 505 ul per batch 1 tube per test 5 1 tube per test if standard reaction tube 2 tubes per test if oryo vial 5 extra Choose Here gt If standard reaction tube is used cryo vial are needed for storage purpose 1 tag par vial 2 tip per tests 5 extra to add supernatent to PCR Tubes and nx 3 DNA 2 tips per test for DNA Extraction 2 tips per batch for amplification 1 tip per batch to add DEN 1 tip per test to add DN4 amplicon iftwincubator 2 tips for SUBCON 5 extra 1 tip per batch for mastermix 5 extra 2 tips to add discard decontaminated specimen only for twincubator 2 tips per batch to add Hybridisation and STR 8 tips per batch to add RINS 3 WATER 3 SUB 1 CONJ 1 Alternative to Long 1ml tips 8 tips per batch to add RINS 3 WATER 3 SUB 1 CONJ 1 5 extra 2 per batch 1 pen for 3 kits 4 tubes per batch diluting COM and SUB HYB STR 3 tubes per batch 5 tubes if GT Blot 48 decontamination 4 per working days 8 per working day 1 kit for 3 LPA kit if GT BLOT 48 1 tray for 10 batches 20 20
12. e per test For inoculation contaminated tube postive tube repeat rate 10 extra 1 per positive 30 of positive 81 position in a box for storage of cryovial 1 petri dish for 4 positive 20 contatminated tubes 1 test for each positive and contaminated 1 pack per year 2 rolls per year CALCULATION DETAILS 5 tubes per test 20 Biosafety and Cleaning With the given period number of staff and the number of Biosafety Cabinets specified on the general settings the system calculates and displays an itemized list of all the Biosafety and Cleaning items required to carry out the exercise for the period as shown below l e with 4 Biosafety cabinets 10 Staff in 12 months you require the following items Biosafety AND Cleaning Items Parameters Items UNIT PACK Hair Cover 2000 Shoe cover 1000 Laboratory coat size L Disposable Sterile 100 Laboratory coat size M Disposable Sterile 100 Laboratory coat size S Disposable Sterile 100 Latex gloves size L 1000 Latex gloves size M 1000 Latex gloves size S 1000 Masks 3M 9320 10 Surgical gowns non sterile Size L 20 Surgical gowns non sterile Size M 20 Surgical gowns non sterile Size S 20 Emergency spill kit 1 First aid kit 1 Cost for BIOSAFETY CLEANING ITEMS UNIT PACK Aluminum foil Cotton wool Plastic bags biohazard waste 30L Plastic bags made from PP 200x300mm Transparent polypropylene waste bag size 420x600mm Single use paper towels
13. ecolourization Hydrochloric acid 2 5L bottle Total amount Cost for Microscopy Applicator sticks if loops are not available pack of 1000 Microscope slides lime soda glass pack of 50 Immersion oil 500ml Lens tissue paper 50 pages block 10 blocks pack Filter paper diameter 150 mm 100 per pack Methylated ethanol for spirit lamps bottle of 2 5 L Marker pen water resistant Sputum containers pack 100 Total amount Cost for Microscopy2 TOTAL PROCESSING TOTAL per sample EXPAND TB Budgeting Application Tool Category Microscopy1 Microscopy1 Microscopy1 Microscopy1 Microscopy1 Microscopy1 Microscopy2 Microscopy2 Microscopy2 Microscopy2 Microscopy2 Microscopy2 Microscopy2 Microscopy2 UNIT PACK 100 100 5000 2500 2500 2500 QUANTITY OF PACKS UNIT COST US 63 CALCULATION DETAILS Carbol Fuchsin Sml per slide includes 10 Methylene Blue Sml per slide includes 10 includes 10 includes 10 Decolourization 7ml per slide includes 10 includes 10 includes 10 includes 10 includes 10 includes 10 includes 10 includes 10 Budget Summary The Budget summary page gives the summary of all the sections on the tool as shown below Dashboard Country KENYA Period of Budget Reagents and Consumables Microscopy Processing for culture Solid culture Liquid culture DST Liquid culture LPA Xpert MTB RIF Sub Total
14. et summary i e time period for which the budgeting is calculated for against the number of staff and the biosafety cabinets Budgeting for different test can be activated or deactivated by selecting YES or NO from the drop down along the test name on the Testing Method section Enter the parameters to set up your lab At the bottom of the page there is more settings link that provides with the system user the option for supplying National Trainings and Supervision Unit Costs and the save button once all the data input has been verified General Settings GENERAL SETTINGS PERIOD in Months 0 01 Number of Staff in the Lab 0 0001 Number of Biosafety Cabinet 0 0001 TESTING METHODS Choose which method you use in the laboratory from the drop down lists below YES NO PROCESSING YES X Which Lowenstein Jensen medium From Component v Which decontamination method NALC NaOH v LINE PROBE ASSAY YES X LIQUID CULTURE YES X DRUG SUSCEPTIBILITY TESTING YES v XPERT MTB RIF YES v MICROSCOPY YES v More Settings Save Settings The More Settings section can be accessed by clicking on the more settings link which opens in a pop up below EXPAND TB Budgeting Application Tool AMORE SETTINGS National Trainings and Supervision Unit Costs Per diems for international experts facilitators per person and per day UN 05 2013 10 Per diems for national experts facilitators per person and per day Per diem for local participants average per
15. for first line drugs and cost for second line drugs Total Processing Solid Culture Cost of Processing cost for first line drugs cost for second line drugs Total per Sample Total Processing Number of Samples for the Period SUMMARY TOTAL PROCESSING 1 418 07 TOTAL per sample 118 17 EXPAND TB Budgeting Application Tool 14 Line Probe Assay LPA LPA Line Probe Assay section can be accessed by clicking on LPA Line Probe Assay on the left navigation to fill in the details on the parameters section The section has a drop down list to select the LPA equipment used in the Laboratory Twincunbator or GT Blot and Batch related data as shown below LINE PROBE ASSAY USING HAIN KITS V2 Items Parameters Average size of a batch Number of batch for the period Total number of specimen to process Number of Test Sub Total Total number of tests to be done for the period 1 Total number of test to order round up to unit per pack of the kit 96 Genotype MTBDRPlus 96 tests kit kits required 1 Number of batch per week 0 0001 Number of specimen in one batch 0 0001 No of controls per batch 0 0001 No of tests coming from Liquid Culture for DST with LPA 0 0001 estimated of repetitions 0 0001 Number of extra test per batch for pipetting error 0 0001 LPA Equipment used in the laboratory TWINCUBATOR Choose if you use Combitips or No Combitips NO COMBITIPS USED SAVE Data supplied above calculates and di
16. i AEE Shang Stop Partnership GLOBAL DRUG de tse A J h f W F K Y Y Za lt 85 LLI Y 3 q 7 ar a x 4 UNIT EXPAND TB BUDGETING APPLICATION TOOL USER MANUAL October 2015 UNIT G Y hb y Stop Partnership GLOBAL DRUG o FIND FACILITY EXPAND TB Budgeting Application Tool TABLE OF CONTENTS INTRODUCTION aan 3 PROJECT E 3 A 5 A eee ee ee eee E ee eer 6 FPA SOW ONC MOS 7 Ac SO 8 INGO SC OO AA A oad E A 10 Processing and Solid Cuiururreeeeeeee 12 Line TARO ASSay LPA 15 MIO OU 666 17 asian eee ee eee eee 19 Biosafety and Cleaning 21 Maintenance and Repal cccccecccscccecccseccecccseceueceueceueceueeeueseusceueseueseueseeeseeeseeeseeesaeees 22 Human e 24 Quay ASS UTN A 24 PR EA 25 DARED PP tie cri te cee PP O PS OE E A 26 Too e Eeen ERED 27 EXPAND TB Budgeting Application Tool INTRODUCTION In an effort to streamline budgeting ordering and management component in laboratories FIND has developed an online budget application tool to reduce complexity and streamline budgeting procedures for ease of planning and decision making at all levels The tool is robust scalab
17. l cost to run the laboratory for the given period The list of the items already prepopulated on the system is as shown below The system has an option for add remove OPERATIONAL COSTS temas Other costs Name Cost Per Month Add ITEM Fuel generator edit delete Electricity 0 edit delete Request forms edit delete Registers 0 edit delete Telephone amp Internet 0 edit delete Dest H20 0 edit delete Total Cost 0 Total Operation Cost 0 00 Create New Section EXPAND TB Budgeting Application Tool Draft Report The Section exports all the data input in all the sections on the system to an Excel sheet The export is section based as shown below Liquid Culture and DST Biosafety and Cleaning Maintainance and Repair HR QA Operations Processing and Solid Culture Microscopy Items LPA Line Probe Assay Xpert Microscopy Items To export data click on the tab on which you want to export data to excel and click on the export to excel button A save as dialog box will pop up for data download to your computer Microscopy Items Processing and Solid Culture LPA Line Probe Assay Xpert Liquid Culture and DST Biosafety and Cleaning Maintainance and Repair HR QA Operations Microscopy Items Export to Excel ITEMS Basic fuchsine 100g bottle Methylene blue 100g bottle Phenol crystals colourless 5kg Ethanol 96 2 5L bottle for stain solutions Ethanol 96 2 5L bottle for d
18. le and flexible to accommodate your current requirement and future enhancement and thus improve efficiency in ordering and budget management while reducing total cost of ownership The EXPAND TB project provided an opportunity for a first application of the budgeting tool which is currently being used to help quantify the resources required to sustain all the activities of laboratories that have been established or refurbished as part of the project including those not currently supported by EXPAND TB such as microscopy human resources and equipment maintenance This will enable to identify resources to support TB laboratory activities in project countries in a sustainable manner after the end of the project PROJECT SCOPE The project provided a fully functional online budgeting tool for FIND that meets the objectives outlined in the Operational requirements The Online tool consists of the following components e Multiuser Capability User Registration through an Email Address e Laboratory General Settings and Budget Summary e Microscopy Processing and Solid Culture e LPA Line Probe Assay Xpert MTB RIF Liquid Culture and DST e Biosafety Cleaning Maintenance and Repair e Human Resource Quality Assurance and Operations e Reporting EXPAND TB Budgeting Application Tool ONLINE BUDGETING TOOL To access the Online budgeting tool enter the below Link to your Browser http www finddiagnostics training org Budget index php auth
19. nage items to add or remove an item as you wish Summation is dependent on the values input Number of equipment unit cost and service cost per year Maintenance and Repair Items Manage Items Parameters Costs for Equipment service amp maintenance contracts Service costs for one Service costs 10 of year USD Value Equipment No of units Unit costs USD Biosafety cabinets Service contract 1 year Biosafety cabinets Spare parts Biosafety cabinets Travelfaccomodation technician Twincubator LPA Service contract 1 year Twincubator LPA Spare parts Thermocyder Service contract 1 year Thermocyder spare parts Thermocyder Travelaccommodation technician Laminar flows PCR workstation service contract 1 year Laminar fiows PCR workstation spare parts Laminar flows PCR workstation travel accomodation Autoclave service 1 year contract Autoclave travellaccomodation Autoclave spare parts BD BACTEC MGIT 960 System service contract 1 year BD BACTEC MGIT 960 System travel and accommodat BD BACTEC MGIT 960 System BD BBL Air Fiters Rect BD BACTEC MGIT 960 System BD BBL Calibrators Kit GeneXpert 4 module service contract after warranty Genexpert 4 module GU Validation kit Total amount EXPAND TB Budgeting Application Tool 22 Costs for equipment repair w out service contracts Value lab equipment Costs for repair 10 Equipment Unit costs USD USD of value Analytical balance 0 00
20. ost for Microscopy1 UNIT COST ITEMS UNIT PACK QUANTITY OF PACKS US CALCULATION DETAILS Sticks ifloops are not available pack of 1000 12 includes 10 Microscope slides lime soda glass pack of 50 50 228 includes 10 Immersion oil 500ml 500 3 includes 10 Lens tissue paper 50 pages block 10 blocks pack 500 includes 10 Filter paper diameter 150 mm 100 per pack 100 includes 10 Methylated ethanol for spirit lamps bottle of 2 5L Marker pen water resistant Sputum containers pack 100 includes 10 Total amount Cost for Microscopy2 SUMMARY Total amount microscopy 3 034 21 Cost per smear 0 29 EXPAND TB Budgeting Application Tool 11 Processing and Solid Culture Processing and Solid Culture section can be accessed by clicking on Microscopy on the left navigation to fill in the details on the parameters section The provision calculates the items required in an itemized format asummary and a detailed calculation description for each section Processing amp Solid Culture ltems Parameters Name Number of slant produced with standard batch Number of Batch for the period Number of slants used for ist line DST Number of slants used for 2nd line DST Total number of slants for DST Number of samples for the period Number of slants used per sample Repetition rate Contamination rate Average volume of L J media per tube Of MTB positive Number of drugs tested for 1s
21. per specimen in average Potassium dihydrogen phosphate KH2P04 MW 136 09 1Kg Sodium hydroxide NaOH MW 40 00 purum 98 pellets 1Kg 409 per 1 liter of specimen N acetyl L cysteine NALC CSHINO3S MW 163 19 puriss 7 99 100g 106 5 5g per 1 liter of specimen Tri Sodium citrate dihydrate C6H5Na307 2H20 MW 294 10 1Kg 69 74 14 5q for 1 liter of final mix buffer 2 an Sodium hydroxide NaOH MW 40 00 purum 98 31 46 20g for 1 Liter of final mix buffer pellets 1Kg PP tubes for centrifuge sterile 50 ml 108 01 1 tube per sample 10 extra Disposable pasteur pipettes graduated non sterile 155 mm 16 87 1 pack per year Total amount 449 73 Cost for Processing 37 4775 EXPAND TB Budgeting Application Tool UNIT COST US UNIT PACK QUANTITY OF PACKS CALCULATION DETAILS You need 1 mi of Solution per batch of 28 tubes 200ml of media containg drug at 7ml per tube 10mg is needed to prepare 100ml of Solution 1 1ml aliquots of Solution DST laboratory chemicals 5g Isoniazid You need 2 5 ml of Solution per 28 tubes 200ml of media containg drug at 7ml per tube 41 2mg is needed to prepare 100ml of Solution Rifampicin Solution needs to be DST laboratory chemicals 1g Rifampicin You need 5 mi of Solution per batch of 28 8 tubes 200ml of media containg drug at 7ml DST laboratory chemicals 25 g Ethambutol per tube 13 6mg is needed to prepare 50mi of Solution
22. person and per day Daily fee for international expert facilitators per person and per day Per diem for support staff per person and per day Travel costs for international experts facilitators per person Travel costs for nationalexperts facilitators per person in per diem Cost of transportation per international expert facilitator Cost of transportation per national expert facilitator in per diem Cost of transportation per participant in per diem Cost of hotel capital per person and per day in per diem Costs of hotel periphery per person and per day in per diem Costs of refresher and lunch per day and per person Cost of room rental per day Cost of stationary and training material per session International Trainings Unit Costs Per diem per person and day Costs for airfare per participant Costs CXC On Data submission the system will confirm by displaying a message whether data input has been saved successfully or not The red warning line Some Parameters have Zero value kindly update them is informational and will disappear once all the fields for a section has been fully populated General Settings Some Parameters have Zero value kindly update them GENERAL SETTINGS PERIOD in Months Number of Staff in the Lab Number of Biosafety Cabinet i EXPAND TB Budgeting Application Tool Microscopy Microscopy section can be accessed by clicking on Microscopy on the
23. rt MTBIRIF test 10 EXPAND TB Budgeting Application Tool 17 EXPAND TB Budgeting Application Tool 18 Liquid Culture and DST Liquid Culture Drug Susceptibility and PZA testing parameters provides an itemized list with the name Quantity and calculation details for Liquid Culture and Drug Susceptibility Testing The system automatically calculates the Cost for Liquid Cultures Cost for Drug Susceptibility Testing and total cost liquid culture amp DST on the summary section as shown below Liquid Culture and DST Items Parameters Name Value Number of total test for the period including contamination and repeat 2 No of TB cultures for the period 1 Expected contamination rate for liquid Media 0 01 Expected repeat rate sub culture 0 01 j of MTB positive culture 0 01 SIRE No of strains to be tested 0 01 Expected repeat rate for SIRE 0 01 PZA No of strains to be tested 0 01 Expected repeat rate for PZA 0 01 EXPAND TB Budgeting Application Tool 19 Items Liquid Cultures ITEMS UNIT PACK QUANTITY OF PACKS UNIT COST US BBL MGIT Tubes for use in Bactec MGIT 960 7ml 100 tubes pkg 100 41 195 BACTEC MGIT 960 Supplement Kit 100 tests PANTA and OADC 100 41 71 combined Culture tubes diameter 16 mm 100 5 35 PP tubes for centrifuge sterile 50 ml 500 9 108 Single use plastic Pasteur pipettes sterile individually packed 500 11 43 Disposable loops 10 500 2
24. splays an itemized list of the items required quantity and pricing per item a total amount for LPA and the total per LPA test as shown below EXPAND TB Budgeting Application Tool 15 ITEMS UNIT PACK Geno Type MTBORplus 99 tests kit j Version 2 0 35 204984 GenoLyse 88 tests Version 1 0 51870 396 Molecular grade water 10x T ml 17 PCR tubes 0 2 mi with attached caps sterile 1000 DNAse RNAse free 1000 per pack Cryo vial sterile with cap 1 5 ml 1000 Cryo vial sterile with cap for one hand 1000 operation Cryo tags sized to fit for use on cryo tubes 1000 free TIPS for pipettes 0 1 10 480 DONAse RNAse free TIPS for pipettes 20 200 280 AApl Sterile DNA RNAse free TIPS 100 1000 Aul 500 Leng 1 ml tips with filter 200 Combitips for Multipette 12 5 ml 100 Disposable pasteur pipettes graduated non 500 sterile 155 mm Forceps plastic 100 Marker pen water resistant 1 PP tubes for centrifuge non sterile 15 mil 500 PP tubes for centrifuge non sterile 50 mi 500 Plastic bags disposable PP 100 pieces per pack 100 Filter paper sheets 100 GTBlot 48 reagent kits 1 GT Blot 48 Tray for strips black 1 Sodium Hypochloride 1 Kg 14 activity 1 Single use paper towels 4500 Ethanol Iisopropanol 1 Liter 5 Cost for LPA test SUMMARY Total Amount for LPA 365 977 99 Total per LPA test 25 50 EXPAND TB Budgeting Application Tool PACKS 13 119 11 11 UNIT COST US5 1008 1
25. t line DST Number of controls for 1st line DST of positive cultures subjected to 2nd line DST Number of drugs tested for 2nd line DST Number of controls for 2nd line DST EXPAND TB Budgeting Application Tool UNIT CUSI US 106259 Ready to use mix 500g 109 42 ready mix LJ 37 5 g per batch ITEMS UNIT PACK QUANTITY OF PACKS CALCULATION DETAILS Glycerol gt 99 purity 1 litre 15 79 12 ml per batch Fresh eggs fed without growth factors antibiotics 0 2 25g per 1600 mi of final solution Potassium dihydrogen phosphate KH2P04 MW 136 09 1 kg 32 49 2 4g per batch Magnesium sulfate heptahydrate MgSO4 7H20 MW 246 48 500g 34 81 0 24g per batch L Asparagine momohydrate C4H8N203 H20 MW 150 13 250g 3 6g per batch Tri Magnesium di citrate nonahydrate 55 Mg3 C6H507 2 9 H20 5009 a7 A Malachite green oxalate 100 g 56 87 0 4g per batch Glycerol 589956 purity 1 litre 15 79 12ml per batch Fresh eggs fed without growth factors free from 41 25 25 per 1600 mi of final solution antibiotics Total amount 287 06 Cost for Solid Culture 23 9217 UNIT COST US ITEMS UNIT PACK QUANTITY OF PACKS CALCULATION DETAILS 4 74 g for 1 liter of final mix buffer 50 extra 84 66 Calculation done for 40 mi of final mix per specimen in average di Sodium hydrogen phosphate anhydrous Na2HPO4 MW 141 96 1kg 4 549 11 for 1 liter of final mix buffer 50 extra Calculation done for 40 mi of final mix
26. t service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Costs for Equipment service amp maintenance contracts Biosafety cabinets Service contract Biosafety cabinets Spare parts Biosafety cabinets TraveVaccomodation Twincubator LPA Service contract Twincubator LPA Spare parts Thermocycler Service contract Thermocycler spare parts Thermocycler TraveVaccommodation Laminar flows PCR workstation Laminar flows PCR workstation Laminar flows PCR workstation Autoclave service 1 year contract Autoclave traveVaccomodation Autoclave spare parts BD BACTEC MGIT 960 System BD BACTEC MGIT 960 System EXPAND TB Budgeting Application Tool o O 8 8 8 8 8 8 8 8 8 amp 8 0 0 0 0 wal acd tones ce Ml ee a ed o oc O 8 8 8 8 8 8 8 8 8 8 8 82 0 9 9 PO Po 9 9 PO PO Po 9 9 PO Po PO 9 23 Human Resources The Human resource section handles the staff management based on cadre and remuneration as shown below Human Resources Human Resource Incentives New f Edit Remove Description No of staffs Annual contract amount per staff Contract Annual Incentive per staff USD Total annual costs USD Head of laboratory 0 0 o Laboratory technicians NRL
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