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HT300A USER MANUAL
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1. 10 11 14 15 15 16 INTRODUCTION e he X Seal Plate Piercing System PPS is a reliable semiautomatic device designed to quickly and correctly piercing of seals on sample plate e An interchangeable piercing head and easily fitted plate adapter allow the PPS to be used with most plates available including standard plates PCR plates and deep well plates with 96 or 384 wells square or round design e The number of perforating cycles required can be selected by the user to suit plate type Easy to use with just three steps e Place the plate on the adapter cover with frame and the piercing head e Close the drawer The piercing process will then start automatically e Open the drawer and remove the pierced plate WARNINGS Before use please read the following warnings carefully e This product must only be used in accordance with proper safety standards and procedures together with the instructions contained in this manual The PPS has been designed and manufactured to conform to international safety specifications e Users should be aware of the potential hazards associated with the equipment e All operators should read and observe the safety precautions and warnings given in this manual before using the PPS e f the PPS is used in a way not specified within this manual any inbuilt protection may not be adequate The following caution and safety signs are used in this manual WARNING Please refer to manu
2. adapter 4 Put the frame on the plate 5 Place the piercing head on top 6 Close the drawer 6 TROUBLESHOOTING INTRODUCTION Possible errors generated by the PPS are summarized in this section If the solutions suggested are not successful please contact your supplier ERRORS Errors are shown on the display and the PPS will emit an intermittent warning sound The amber LED will be off The errors are Er The system is unable to reach the home position within a defined time Er2 he motor cannot achieve the compression force within a defined time Er3 The motor cannot reach the home position activating the home sensor within a defined time Er4 The system has detected a motor overload prior fo reaching the home position Er5 open drawer sensor active during rise movement Er Home position sensor is not activated during the piercing operation within a defined time If Er2 is displayed first check that the adapter plate frame and piercing head have been loaded correctly If Er5 is shown the error may be caused by the drawer not being pressed in for the amount of time required to lock it Retry Other errors may be rectified by switching the PPS off then on again and retrying 7 MAINTENANCE PERIODICAL MAINTENANCE The PPS does not require servicing but should be cleaned regularly e The PPS can be cleaned with a cloth dipped in water or ethanol methanol formaldeyde can al
3. run cycle 5 BASIC FUNCTIONS RUN CYCLE Turn on the system The display will show the firmware release information The unit will automatically go to the home position with e Active open drawer sensor and motor home sensor activated e he drawer will be released The display shows Rdy indicating that the system is ready for piercing The amber LED will remain on Open the drawer and load the adapter Place the plate over the adapter Place the frame over the plate and then place the pierce head above Close the drawer The loading procedure is explained further in the next section Keep the drawer pressed until the unit emits an audible warning to indicate that the close drawer sensor is activated and the drawer is locked The unit moves the plate amp piercing head assembly upwards applying an appropriate pressure During piercing operation the display will show Bsy and the amber LED will flash When the set piercing cycles are completed an audible warning sound is emitted and the display will show Rdy The amber LED remains on but not flashing The unit moves the plate amp piercing head assembly back to the home position and the drawer is released The operator can remove the pierced plate A new cycle can be started LOADING PROCEDURE The following steps should be used to load the PPS 1 Open the drawer 2 Place the adapter inside the drawer 3 Place the plate over the
4. Operating Instructions For The X Seal Plate Piercing System Version Release 1 Nov 2009 FluidX Ltd FluidX Ltd Monks Heath Hall Chelford Road Nether Alderley Cheshire SK10 4SY UK T 44 0 1625 861 614 F 44 0 1625 861 615 E infoQfluidx co Uk www fluidx co uk Operating instructions for the X Seal Plate Piercing System This document is for information only the manufacturer accepts no liability for errors contained herein or for incidental or consequential damages with the furnishing performance or use of this material Unless otherwise specified references to names or parts is purely casual and has the purpose of illustrating the product The contents of this publication may not be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from the copyright owner The information contained in this document is subject to change without notice All rights reserved TABLE OF CONTENTS 1 INTRODUCTION WARNINGS APPLICABLE RULES SPECIFICATIONS 2 GENERAL DESCRIPTION MAIN COMPONENTS CONTROL PANEL REAR POWER INTERFACE 3 INSTALLATION PRELIMINARY VOLTAGE CHECK TO CHANGE THE VOLTAGE SETTING 4 SETUP TO SET UP OPERATING MODE 5 BASIC FUNCTIONS RUN CYCLE LOADING PROCEDURE 6 TROUBLESHOOTING INTRODUCTION ERRORS 7 MAINTENANCE PERIODICAL MAINTENANCE 8 ACCESSORIES 9 DISPOSAL
5. al e he machine is designed for indoor laboratory use only at an altitude of less than 2200m above the sea level within a temperature range of 15 C to 35 C and a relative humidity range of 576 to 8576 non condensing If the instrument is stored outside these ranges it should be left to stand until it equilibrates to within the above limits e Ensure that the voltage selection switch is set to the correct voltage and that the correct fuse for the required voltage setting is fitted Do not work outside the rated power supply range e Use the cleaning method recommended by the manufacturer e Ensure the unit is only connected to an earthed supply e There are no user accessible or serviceable parts inside the unit Do not remove the unit s cabinet WARNING Risk of electrical shock high voltage e his equipment should only be dismantled by properly trained personnel removing the top case exposes potentially lethal mains voltages APPLICABLE RULES 2006 95 CE Low Voltage Directive 2004 108 CE Electromagnetic Compatibility Directive 2002 96 CE amended 2003 108 CE WEEE Directive SPECIFICATIONS Height Depth Width Weight Voltage Frequency Power Temperature range Relative humidity 310mm 275mm 181mm 10kg 230 115 100 410 Vac 47 63Hz 100W Operative 18 C 35 C Storage 20 C 40 C 20 80 not condensing 2 GENERAL DESCRIPTION MAIN COMPONENTS Figl e Cabinet e Control panel
6. e Rear Power Interface e Electronic circuit e Handling system motor sensors e Plate adapter e Plate e Frame e Metallic pierce head CONTROL PANEL pierce Boo Fig2 Amber light Status indicator M key Set up Run mode key Three Digit LED display Q Directional key up Cc A O N Directional key down REAR POWER INTERFACE FIGS Power Setting 115 230 Vac and Fuse Holder 2 I O Power Switch 3 Main Power Plug 3 INSTALLATION PRELIMINARY After unpacking make sure all packing and fixtures are retained as the unit must always be transported in the original packing to avoid damage The manufacturer accepts no responsibility for damage incurred unless the unit is correctly packed and transported in this way Remove the PPS unit from its packaging The PPS must be placed on a level surface away from direct sunlight ensuring access to the power switch on the back of the unit Ensure that the vents on the cabinet are not obstructed The main voltage selector switch should be checked to ensure that the voltage has been set at the correct value Connect the unit to the main power supply and switch on the switch I O of the rear power interface VOLTAGE CHECK Ld Be sure that the equipment is not connected to the mains power On the back of the PPS there a connection panel with Power setting 230 115 Vac and fuse holder On off power switch Main power plug The setti
7. ht The appropriate separated collection allows recycling treatment and disposal of the unit It reduces negative effects on the environment human health and allows the unit to be recycled where possible Be aware that the abusive disposal of the product causes the application of administrative sanctions according to the laws in force Please contact your supplier for further information Manufactured for fluidX Ltd Monks Heath Hall Helford Road Nether Alderley Cheshire SK10 4SY UK Tel 44 0 1625 861 614 Fax 44 0 1625 861 615 Email sales fluidx co uk www fluidx co uk Manufactured by HTA Srl Via del Mella 77 79 Brescia Il 25131 Italy Tel 39 0 030 3582920 Fax 39 0 030 3582930 www hta it com
8. ng voltage is written in white on a red background on the top of power setting and fuse holder The PPS is factory set to 230V co d w Mee l w a ow whip C M wr IN 4 Fig4 TO CHANGE THE VOLTAGE SETTING E E gt da vum i eee DES Le E f z P Fig5 e Open fuse holder compartment with a screwdriver fig 4 e With the screwdriver extract the fuse holder fig 5 e Replace the two fuses with two with the following characteristics see the table below Power Voltage EN 60127 e l5v e 230v e 2A T e 1A T e Insert the fuse holder keeping the set voltage label at the top e Close the fuse compartment pressing gently e f the fuse is correctly inserted the voltage selected will be seen 4 SET UP TO SET UP OPERATING MODE Turn the PPS on To enter the Set up mode press and release the M key The display will show a flashing S and the last set buzzer function The buzzer function can be enabled on or disabled no using the O keys Pressing the M key again to store the audible warning set It is then possible to set the cycle number The display will show a flashing c and last set cycle number The cycle number can be decreased or increased using the O keys C can be set between to 10 with steps of 1 Press the M key again to store the new cycle number value and exit from the set up menu The unit will return to the
9. so be used e The unit should not be immersed in solvents e Do not use acetone or abrasive cleaners e No parts are to be autoclaved e Incase of radioactive spillages it is recommended to use an appropriate cleaning agent e For any other maintenance operation not indicated in this manual please contact your Supplier Any cleaning procedure must be performed with the power OFF and the power cable disconnected 8 ACCESSORIES The PPS is designed to be used with the following accessories Plate adapter 6 09 553 Adapter for standard plate 6 09 556 Adapter for 96 PCR plate 6 09 30 Adapter for deep well plate Frame 6 09 550 PPS Frame Metallic piercing head 6 09 555 Metallic piercing head for 384 SBS round well Ask Metallic piercing head for 96 SBS round well Metallic piercing head for 384 SBS square well Ask Metallic piercing head for 96 SBS square well 9 DISPOSAL According to the Directive 2002 95 CE 2002 96 CE and 2003 108 CE concerning the restriction of the use of certain hazardous substances in the electrical and electronic equipment and the waste disposal The symbol of the crossed bin shown on the equipment or on its packaging indicates that the product is to be collected separately from the other waste Thereof the user must deliver the equipment to the appropriate collection points for electrical and electronic waste or to his sale representative if a new similar equipment is boug
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