Installation Manual
Contents
1. 20 KTAFPLC Installation Guide 18 1140 46 Edition AC Installation test di 19 Select the following curves to be displayed e UV e Conc Clear all other highlighted curves Click OK 11 When thetest run is finished the printer automatically prints the chromatogram and the test result Installation Test01 1 Gradient result Step response result gt AKTAFPLC Installation Guide 18 1140 46 Edition AC 21 6 Installation test 22 6 3 Evaluating the installation test results 6 3 1 Automatic evaluation The system automatically prints the test result when the test is finished The print out consists of a chromatogram and an evaluation of the test result Ifthe gradient test result is O K the print out says Gradient linearity accepted If the step response test result is OK the print out says Step response accepted If any of the evaluated values fall outsidethe specified range go to step 6 4 Correcting faulty evaluation results 6 3 2 Manual evaluation If your chromatography system deviate from the standard configuration e g if optional components have been installed the automatic evaluation will not give a reliable result If so perform a manual evaluation as described below 1 Select the UNICORN Main menu module 2 Click on in the Results window and then double click on the Wizard Generated001 icon to open the result file 3 Click Print to obtain2. 28 AKTAFPLC Installation Guide 18 1140 46 Edition AC Registration form 9 KTAFPLC Installation Guide 18 1140 46 Edition AC 29 EB Registration form 30 AKTAFPLC Installation Guide 18 1140 46 Edition AC Amersham e Biosciences TC information Uppsala Printed in Sweden by TR i Uppsala AB
3. KTA design AKTAFPLC Installation Guide Amersham 18 1140 46 3 e Biosciences Important user information All users must read this entire manual to fully understand the safe use of AKTAFPLC WARNING The WARNING sign highlights instructions A that must be follovved to avoid personal injury Do not to proceed until all stated conditions are clearly understood and met Caution The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment Do not to proceed until all stated conditions are clearly understood and met Note The N ote sign is used to indicate information important for trouble free and optimal use of the product CE Certification This product meets all requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked Amersham Biosciences instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from Amersham Biosciences except for alterations described in this manual WARNING Thisisa Class A product In a domestic environment it may cause radio interference in which case the user may be required to take suitable measures
4. 6 Installation test ria L7 6 1 Preparation of AK TAPP EC Lasi 6 2 Running the installation test method 6 3 Evaluating the installation test results 6 4 Correcting faulty evaluation results i 7 T strecornd inni E 7 1 Gradient test result arrere anna 24 7 2 Step response test result ccc cece teste tees teeetetteetetnae 24 8 Installation record ria 25 9 Registration form visscssscsssesceeeeeeseeeseueenneennneny 2 7 9 1 Components ae 28 KTAFPLC Installation Guide 18 1140 46 Edition AC 5 Contents 6 AKTAFPLC Installation Guide 18 1140 46 Edition AC About this installation guide ER 1 Aboutthis installation guide KTAFPLC is assembled and fully tested before shipping For safe transportation however some components have been secured and need to be released from strappings Extra capillaries accessories column holders etc are enclosed in separate paper boxes This guide describes how to install KTA FPLC It is divided into two parts one describing the installation and one describing how to run the installation test After the installation procedure has been performed your AKTAFPLC is ready for purification work For full details of specifications methods maintenance etc refer to the respective U ser M anuals and Instructions KTAFPLC Installation Guide 18 1140 46 Edition AC 7 ED 2 Safety BB PRD SD e The system is designed f
5. e 5 and 95 faulty bad sealing in pumps 5 faulty pump module B 95 faulty pump module A rr ii KTAFPLC Installation Guide 18 1140 46 Edition AC 23 Test record 7 Testrecord 24 Date aa i 7 1 Gradient test result Gradient linear from B tO 06 B 10 90 7 2 Step response test result Step response table 1 2 3 4 5 Programmed Value Baseline Normalised Allowed Conc B read corrected value interval value 100 95 94 96 70 69 71 30 29 31 5 4 6 0 AKTAFPLC Installation Guide 18 1140 46 Edition AC 8 Installation record Check Sign Remarks 1 Unpacking e Contents according to packing lists All packing material removed e No visible damage 2 Installation Injection valve waste tubings port 4 and 5 marked W1 and W2 to waste reservoir e Fraction collector unpacked and installed Outlet tubing from flow restrictor connected to fraction collector Outlet waste tubing extended to waste reservoir Computer and printer installed e UniNet 1 cabling installed e Mains power cabling installed 3 Installation test Solutions prepared e AKTAFPLC prepared Installation Test method run Installation Test results evaluated Test Record completed e Registration Form completed Test Record and copy of Registration form stored in System Logbook Registration form posted to Service Adm
6. Guide 18 1140 46 Edition AC 9 ED Pre requisites 3 Pre requisites socket WARNING AKTAFPLC must be connected to a grounded mains Two people are required to lift AK TAFPLC onto the working bench To install AKTAFPLC a working area of about 200 x 80 cm is required AKTAFPLC requires 100 120 220 240 V 50 60 Hz electrical supply with safety grounding e Cutting pliers are recommended for cutting plastic straps e A waste flask is needed e The installation test requires the following solutions 200 ml of distilled water 200 ml of 0 4 acetone in distilled water 10 AKTAFPLC Installation Guide 18 1140 46 Edition AC Installation overview I 4 Installation overview e Unpack KT TAFPLC bestseller 12 e Detach packing material and unstrap itemS 13 e Unpack and install the computer ete etter etter 15 e Connect mains power cabling i 15 e Connect UniN et 1 data communication chain cabling 16 e Complete the first two sections of the installation record 25 e PrepareAKTAFPLC for the installation test 17 e Run the installation test Method 0 eee iaia 18 e Evaluate the gradient eccerre 22 e Evaluate the step response 23 e Complete the test record 24 e Complete the registration form iii 27 e Complete the final section of the installation record 25 Store ph
7. Terms and Conditions of Sale Unless otherwise agreed in writing all goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Should you have any comments on this instruction we will be pleased to receive them at Amersham Biosciences AB SE 751 84 Uppsala Sweden Trademarks Drop Design AKTA AKTAFPLC Superloop and UNICORN are trademarks of Amersham Biosciences Limited Amersham and Amersham Biosciences are trademarks of Amersham plc Office Addresses Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH M unzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences K K Sanken Building 3 25 1 H yakunincho Shinjuku ku Tokyo 169 0073 Japan Amersham Biosciences Limited 13 F Tower Ever Gain Plaza 88 Container Port Road Kwai Chung New Territories HongKong www amershambiosciences com Copyright Amersham Biosciences AB 2003 AII rights reserved Contents Contents 1 About this installation guide ia 7 2 Safety ici 8 3 Pre requisites cicci 10 4 Installation overview wuss LI 5 Installation of AKTAFPLC ssis 12
8. AFPLC chromatography system with the mains switch located to the left on the AKTAFPLC system pump P 920 2 Switch on the computer and the printer 3 Checkontheoptical unit that the UV wavelength is set to 254 nm If not set the wavelength by selecting lamp position indicated by a dot on the lamp housing in combination with the appropriate filter i e the dot on the lamp housing should be adjacent to the symbol on the filter housing corresponding to LED the symbol on thefilter wheel for the 254 nm filter A click will el Filter wheel indicate that the filter is in 8 labels position Re filter housing KTAFPLC Installation Guide 18 1140 46 Edition AC 17 6 Installation test E 4 Check that the inlet filters are submerged in the corresponding buffers A and B Method Guide Buffer A Distilled water 200 ml Buffer B 0 4 acetone in distilled water 200 ml Flow rate 5 ml min Test run time Approximately 25 minutes 6 2 Running the installation test method 1 Start UNICORN as described in the M aking your first runs booklet 2 InUNICORN select File Printer Setup Select the appropriate printer from the list and select Landscape Then click OK to acknowledge the printer chosen Print Setup RE m Printer Name SEUPPBT_FS1 BL52_D_HP4000_Q Properties Status Ready Type HP LaserJet 4050 Series PCL Se Where SEUPPBT_FS1 BL52_D_HP4000_0 Comment Pape
9. UniN et 1 cable to the fraction collector To PC controller board UniNet 2 chain Four LEDs UniNet 1 chain CAUTION The UniNet 1 connection to the computer MUST be made to the board with four LEDs 23 Connectthelonger UniN et 1 cableto the controller board installed in the computer WARNING RTAFPLC must be connected to a grounded mains socket 24 Connectthe system mains cable from the mains inlet to a properly grounded mains socket 25 All other cables are connected at delivery 26 Check all cable connections 27 Turn AKTAFPLC so that the front is facing forward 28 Complete the first two sections of the Installation record on page 25 29 Theinstallation phase of AKTAFPLC is now completed AKTAFPLC Installation Guide 18 1140 46 Edition AC Installation test di 6 Installation test The installation test checks the function of the liquid delivery and the UV monitoring system of AKTAFPLC The installation test can also be used at any time to check the condition of the system e g after a prolonged stop Correct gradient formation is tested by producing a linear gradient and a series of concentration steps of acetone Correct UV monitoring is tested by monitoring the acetone concentration at 254 nm 6 1 Preparation of AKTAFPLC 1 Switch on the AKT
10. a printed report of the result Evaluating the gradient Place a ruler along the gradient part of curve Wizard Generated001 1_UV 01 SMTH in the printed report The curve should be linear between 10 B and 90 B and void of discontinuities AKTAFPLC Installation Guide 18 1140 46 Edition AC Installation test di E Evaluating the step response Calculate the relative adsorption plateau heights for curve Wizard Generated001 1_UV 01 SMTH as follows 1 Subtract the base line value 0 B from each of the values in column 2 in the Step response table of the Test record see page 24 and enter the results in column 3 2 Divide each value in column 3 by the baseline corrected value corresponding to 100 B multiply by 100 and enter the results in column 4 The values of column 4 should all fall within the intervals given in column 5 6 4 Correcting faulty evaluation results Should any of the evaluated values fall outside the specified range proceed as follows e If the system differs from the standard configuration evaluate the result manually If the faulty evaluation result remains continue below 6 4 1 Faulty gradient e The gradient is linear but the interval is too small M ixer chamber too large or faulty mixer e Disturbances may arise from air in the pump pump valves or bad sealings in the pump Refer to the Pump P 920 User M anual 6 4 2 Faulty step response e Ifall values are faulty air in pump or faulty pump
11. inistration Installation Guide stored in User Manual box for future use KTAFPLC Installation Guide 18 1140 46 Edition AC 25 EB Installation record 26 KTAFPLC Installation Guide 18 1140 46 Edition AC Registration form 9 9 Registration form IMPORTANT WARRANTY REGISTRATION INFORMATION Please ensure that this form is completed and returned to Service Administration to register the users equipment under warranty Name Institute COMPANY ri Address nnn nnn nnn een Department location rina Post Code ria Phone N umber sssssssssnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna Fax Number rin End Users E Mail sees Date of Installation rr Quote N OF ssssssssnsnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Customer O rder N01 rr INVOICE N OF sssssssssnnnnnnnnnnnnnnnnnnnnnnnannnnan Support Agreement purchased with the instrument Y N If M ES give detallSt iana tania aaa Installer name ai tile al iii Note Fill in serial numbers over leaf KTAFPLC Installation Guide 18 1140 46 Edition AC 27 EB Registration form 9 1 Components AKTAFPLC system serial NUMDEFS avan anaren as QTY Part Number Description System rack III Serial Number Mixer M 925 Monitor UPC 900 Pump P 920 Fraction collector INV 907 Computer Computer display
12. ir mounting rails Remove all red tape holding capillaries Place the waste capillaries marked WlandW2ina waste bottle and place the bottle in a convenient location Noton top of the system L To fraction collector Fit lower tray here Take out the inlet tubings with filters from the plastic bag on top of the system KTAFPLC Installation Guide 18 1140 46 Edition AC Installation of AKTAFPLC Ea Take the lower tray stored on top of the system O pen the system Ve rack using the gate handle and fit di the lower tray in its recess 19 Remove all red tape holding the 77 cables Black cables are mains cables Grey cables are UniN et 1 communication cables 20 Connect the mains cable from the system mains outlet T to the fraction P collector mains inlet 21 Unpack and install o OUE GERO the computer and printer according to Free mains the manufacturer s o ni instructions Place o Suprem them to theleft of the system Do not switch them on To fraction collector From mains outlet CAUTION The mains power to AKTAFPLC must be switched OFF before the UniNet 1 cabling is installed AKTAFPLC Installation Guide 18 1140 46 Edition AC 15 EB Installation of ARTAFPLC 16 To fraction collector 22 Connect the shorter
13. on Guide 18 1140 46 Edition AC ir i 6 10 Installation of AKTAFPLC Ea Remove the plastic Graeber cover from the _ i n system by gently ARE 4 tilting the system backwards and grasping it below the system rack Pull out the plastic cover as far as possible Then gently tilt the system forwards grasping it at the top rear and pull away the plastic cover completely Save all the original packing material If the equipment has to be re packed for transportation or otherwise it is important that the system can be safely packed using the original packing material O pen the system Plastic foam rack by lifting the gate handle and swinging out Remove the plastic foam packing Close the system rack SUS Gate CER Es Lift and swing out AKTAFPLC Installation Guide 18 1140 46 Edition AC 13 CD Installation of AKTAFPLC 11 12 13 14 15 16 17 14 Turn the system so that the component side is facing you Plastic foam Injection valve Mixer Lift the optical unit gently to remove the plastic foam packing Cut and remove the plastic straps holding the mixer and the injection valve Lift off the mixer and theinjection valve one at a time to remove the plastic foam packing behind them and then refit Position the mixer injection valve and optical unit as illustrated by pushing them sidew ays in the
14. or indoor use only e Do not usein a dusty atmosphere or close to spraying water Refer to Technical Specifications in the System M anual for detailed environmental pre requisites WARNING The individual instruments must not be opened by the user They contain high voltage circuits that can give a lethal electric shock WARNING Monitor UPC 900 uses high intensity ultra violet light Do not disconnect the optical unit while the lamp is ON WARNING ARTAFPLC must be connected to a grounded mains socket WARNING There must always be a sample loop or Superloop connected to ports 2 and 6 of the injection valve This prevents liquid spraying out of the ports when switching the valve which is especially dangerous if hazardous chemicals are being used WARNING Two people are required to lift the system WARNING Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining and servicing the system AKTAFPLC Installation Guide 18 1140 46 Edition AC sE WARNING If the system is turned around or the fraction collector removed the external capillaries and other tubings may become entangled in nearby objects and be pulled from their connections causing leakage WARNING Never place waste containers on the top of the system If they become full and overflow liquid may penetrate the system causing a short circuit gt Bb KTAFPLC Installation
15. otocopies of all records and forms in the System Logbook e Store the Installation Guidein the User M anual box KTAFPLC Installation Guide 18 1140 46 Edition AC 11 EB Installation of AKTAFPLC 5 Installation of AKTAFPLC Begin by creating a clean and dry working area of 200 x 80 cm that allows easy access Then follow the step by step instructions below and fill in the Installation record as you go along see page 25 Note Some items are packed in separate boxes delivered with the system Note Some packing lists are included in the boxes 1 Afterremovingthecardboard hood and other packing 5 material check the contents against the enclosed packing list Also check all enclosed boxes Store all the boxes and plastic bags in a convenient nearby place ZI Packing list 2 Put aside the fraction collector to unpack later Note The installation of the fraction collector is described in detail in the AKTAFPLC O ptional Configuration User M anual supplied Refer to the section that describes your fraction collector N WARNING Two people are required to lift the system 3 Lift AKTAFPLC onto the work area using the four red US strap handles 4 Release the two red straps with the strap handles and remove 5 Open the plastic cover from the top and fold down to uncover the system Take care not to damage any capillaries or components while doing this 12 AKTAFPLC Installati
16. r Orientation Size Ad Portrait Source Auto Select Landscape Network OK Cancel Nawo ea 18 KTAFPLC Installation Guide 18 1140 46 Edition AC Installation sE E 3 Click the Instant Run button Ee The Instant Run window opens InstantRun Select the appropriate system and click Run 4 Select Installation Test in the M ethod Wizard Click Run Method Wizard for System FPLC_Frac KTAFPLC Installation Guide 18 1140 46 Edition AC 19 6 Installation test E 5 Jn the Evaluation Procedures window select the procedure for your system for example InstTest_FPLC Evaluation Procedures x Selected Evaluation Procedures will run at the end of the method Cintegrate Full Report Report Chromatogram Clintegrate and Print I Print Chromatogram IChromatefocusing 6 Click Next in the Method Information window 7 Click START in the Result Name window to start the installation test T he progress of the test is monitored in the System Control module The installation test run time is approximately 30 min 8 To customize the Curves pane right click in the pane and select Properties 9 Click the Curves tab x YAxis Curve Style and Colour Flow Scheme Logbook Run Data Groups Run Data Colour Curves X xis r Display curves UV E o Cond Clear All MConc OpH Pressure Flow Temp
Download Pdf Manuals
Related Search