GeneMax Instruction Manual
Contents
1. user002 user user CHAPTER VI FIRMWARE UPGRADING 6 1 Connect to PC Connect the equipment with computer by network cable Turn on the equipment power to make the equipment on standby Computer Instrument 6 2 Network Enable Administrator setting 1 Run Network Enable Administrator tool software When upgrading the instrument communicate with virtual device mapping used N Network Enabler Administrator Configuration File Function Configuration View Help Ia 2O nNn Function Configuration 0 Network Enabler Module s BA Network Enabler Admi No Model MACAddess IPAddess Configuration dk COM Mapping Monitor Port Monitor 9 IP Address Report Message Log 0 Monitor Log 0 No Time Description Now 2008 1 3 14 15 25 30 Instruction Manual of GeneMax Thermal Cycler N Network Enabler Administrator Configuration 2 Click search button Or right click Broadcast VER rer teser Vere Search option in Configuration the tool will 2a2 on automatically search for the equipment Function Configuration 1 Network Enabler Module s B Network Enabler Admit Status Configuration NE 41105 OU 90 E9 23 FF 42 192 168 0 254 lig COM Mapping IE Monitor EA Port Monitor2. COM Number COM3 current assigned 22 5 4 Run software Comprise user management file management le o oe Bio ds ld Rename Delete Copy and equipment management etc 23 Instruction Manual of GeneMax Thermal Cycler Export Create Menu bar Instrument contents File display and edition area Display area of running status Status bar Et RE J ss 5 4 1 User management Include creating renaming deleting copying gt pasting importing and exporting user etc d re ax ao ER w D Rename Delete Copy Export User operation 1 Create user Select equipment name sub item in equipment list Create user and click create f Or right click create user The user name shall not be empty The max length of user name is 12 bytes The user name shall only contain numbers and letters under half width mode s OK A same user name cannot be used for more than one time in a same instrument Do you want to create new user in Computer Enter new user name The user name can contain only letters and numbers 2 Rename Select the user needing to be renamed in the equipment list and click rename or right click alter user name Click OK to save after inputting user name It cannot be renamed when the file under the user name is opened Z OK Change user name Do you want to change user name
3. 4 3 6 Screen calibration Click screen calibration fag on the main menu of system setting to enter screen calibration setting screen In the setting process you can finish screen calibration function by clicking upper left corner of the screen and figure center at the lower right center in turn with Max pen as per the prompt Screen calibration interface Backlight Hotlid Close cover while module temp below Keep module temp When cover heating EIEN Eie BEN M Key Beep M Alam Beep End Beep Please click the central point of the figure with a touch pen Please click the central point of the figure with a touch pen Instruction Manual of GeneMax Thermal Cycler 4 3 7 USB Insert USB and click USB EJ on the main menu of system setting The equipment will automatically search for U disk The user can export file to the U disk USB Disk O Input File Output File after successful searching 4 3 8 TM On the main interface of system settings click TM to calculate TM 4 3 9 communication Click communication 2 on the main menu of system setting to enter connunication setting interface Click OK to save after setting communication Network M Bluetooth 4 4 Distribution characteristics of gradient module The gradient module adopts 2 temperature control units The temperature gradient distributes Gradient graphs like a curve See as the following o
4. r BIOER 3337 TECHNOLOGY Genemax Thermal Cycler INSTRUCTION MANUAL Caution The Bioer Co reserves the right to modify this manual at any time without notice The manual contains copyright protected and patentea material Without prior written consent from Hangzhou Bioer Technology Co Lid any part of the manual shall not be duplicated reproduced or translated into any other language Before initial use of this instrument please carefully read this manual Product Patent No ZL 20092019972313 Product Standard No YZB Z 0189 2010 The product is for scientific research only Document No BYQ606700000000ESM Document Version February 2013 Version 1 7 Instruction Manual of GeneMax Thermal Cycler IMPORTANT NOTES 1 General Practice Caution Important information is contained in any item and should be carefully read Failure to observe the instruction would result in damage or abnormal functions to instrument Warning The warning message requires extremely careful operation of a certain step Failure to observe the instruction would result in serious personal injury 2 Safety During operation maintenance and repairment on this instrument the following basic safety notes shall be observed In case of failure to follow these measures or the warnings or notes indicated herein the basic protection provided by the instrument its safety criteria of design and manufacture and its predicted use range would be
5. 72 If 9404 f 9501 If 9550 MH 95h MH 95H_CYCLETE2 2 HH 144 H 4n HH 55 J 95H_CYCLETES New File Page1 of 2 gt Ww ae de de amp Select User New User Delete User Rename Option interface for users Under the main menu clicks run to enter option interface for users as the above figure In the option interface for users the user can create new user delete user and rename etc Main 97 System Main menu in program operation 4 Pagel of 2 Es ES wtf Run New File Edit File Delete File Back Main Option interface for program file Click corresponding user name to enter option interface for programs as the above figure In the option interface for programs the user can create new file edit file delete file and run selected file etc Instruction Manual of GeneMax Thermal Cycler 4 3 Set system parameters Click system setting 4 in the main menu to enter main menu of system parameter setting Click Max screen to enter corresponding system setting interface Ce m OM N DA W Date amp Time Language Backlight Heat Cover Date amp Time Language Backlight Heat Cover ER E el Touch Screen USB Tm calculator Touch Screen Tm calculator Communication fy A Main Main Main menu of system setting Normal version Main menu of system setting Senior version 4 3 1 Date amp Time Click date amp time on the main menu of Date amp Time system
6. 0 99 min Inactivity Timeout Max Connection 0 0 65535 ms Data Packing Optional Delimiter 1 O ff Hex Delimiter 2 O ff Hex Force Tx Timeout 0 65535 ms JA OK X Cancel Operating Mode setting 3 Click Serial option and set in the pop up Serial E Settings window The detailed parameters are as follows Click OK after finishing setting Pat Number 1 Parts Selected 1st port is Port 2 Basic Settings Advanced Settings Serial Parameters 4 Click OK to finish Configuration setting Baud Rate 38400 pl Party None id Data Bits B oe stop Bits 1 2 Flow Control None l Apply All Selected Forte Serial Setting wf OK XM Cancel 4 Click COM Mapping and Add target to add target instrument Double click the added instrument in the list of COM Mapping COM Port Settings dialog box will appear Set COM port in Basic Settings change Mode to Classical in Advanced Settings set Band Rate to 38400 in Searial Parameters then click OK to finish settings Click Apply Change i to save changed setting x CON Port Settings vi ex ss 1 Port s Selected 1st port is Port 1 Port Number 1 Ports Selected 1st port is Port 2 Port Number 1 Portis Selected Tst post is Port 1 Apply port alias to all selected port Port Alias Basic Settings Advanced Settings Serial Paramet
7. copy Bj Or right click copy file in the menu to copy the file If the file is being edited then it cannot be copied 7 Paste Select the equipment option in the equipment list and click paste E or right click paste copied file in the menu to paste the program file under the user and the user name if the copied user has already existed then it will prompt whether to cover in pasting If the program file under the existed user name is being used then it cannot be pasted You can use mouse to drag and paste 8 Import file Click import ad and select the folder of the user in the amp open pop up window Click OK to import file Germ v ep Search Desktop P If there is same file name then it will prompt whether to cover ie enter Desktop i IN Importing d Downloads i i SG Recent Places n Network If the file with the same name is in use then it cannot be ua System Folder i Libraries imported E Documents dre ag eee od Music ks Pictures newpcr110627 EG Videos File folder test ear f Computer 5 amp OS C 9 Export file Click export dl and select the folder saved by the user in the pop up window Click OK to export file Please select export file s target path If there is same file name then it will prompt whether to cover in exporting b a Libraries If the file is being edited then it cannot be
8. Message Log 1 Monitor Log 0 No Time Description oS O 2011 8 24 13 09 24 Found Network Enabler Module s 1 Now 2011 8 24 13 15 58 h 3 Double click the network number searched in list Configuration column to enter configuration setting page like the oman putoWaring IPAddessRepot Password DigtalO SeiaCMD right figure Eie Wy Serial OperaingMode AccessiblelPs 1 Click Network option and select Modify Input pa aai IP Address 192 168 0 245 IP address and subnet mask EA The instrument IP address shall be in the same F Nur network segment with computer IP address For Veda IP Configuration Static example if the computer IP is 192 168 0 1 then the BDS ver ee instrument IP address shall be set as 192 168 0 XXX T Modiy Among them the range of XXX is 2 254 and io i a cannot be the same with computer IP Click the Modify check box to modify configuratio Af OK X Cancel IP address setting 2 Click Operating Mode option and select Operating Mode Modify Click Settings then an operating 1 Port s Selected 1st port is Port 1 mode for selection will pop up Select Real COM ke ME da Mode or TCP Server Mode and click OK TCP Server pane ede UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout
9. all Computer Importing ee b amp OS C If the program file under the same user name is in use the user gt e DATAPARTI D cannot be imported gt 3 DVD Drive E 7 Export user Click export amp to select the folder saved by the user Click OK to export user If there is same user name then it will prompt whether to cover in export If the program file under the user is being edited then the user cannot be exported 5 4 2 File management Include creating renaming deleting copying pasting importing and exporting user etc ut Save Change Rename Delete Copy Pasi mport Export password DU ETP Cae File operation 1 Create Select the user that needs to create file and click create Input file name and password and click OK Create file Do you want to create new file in test of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only letters and numbers 24 25 Instruction Manual of GeneMax Thermal Cycler 2 Edit and save Select the file that needs to be edited and click edit or right click edit file Click save l in the menu bar after finishing edition 3 Alter password Select the file that needs to alter password and click alter password g Input old and new passwords in the pop up dialog box and click OK
10. any portion of body shall not be contacted with metallic portion module or hot lid nearby the said mark otherwise skin would be burnt 4 Maintenance of instrument In order to ensure full contact and good heat conduction between the test tube and the conical holes the conical hole on the module shall be regularly cleaned with clean soft cloth soaked with a little absolute alcohol The stained surface of this instrument if any shall be cleaned with soft cloth soaked with cleaning paste Warning During cleaning the instrument its power supply shall be cut off The instrument surface shall not be cleaned with corrosive cleaning agent 5 After sales service the warranty content and scope are shown in the warranty sheet Warning After unpacking immediately check the goods against the packing list In case of damaged or missed goods please immediately contact the supplier After qualification of acceptance properly fill the product acceptance sheet and send or fax the copied sheet to the supplier for filing and maintenance After unpacking the packing box and packing materials shall be well kept for future repairment For the damage of instrument occurring during transporting to the repair department due to improper package Hangzhou Bioer Technology Co Ltd shall bear no liability Instruction Manual of GeneMax Thermal Cycler CONTENTS CHAPTER INTRODUCTION WEL TEL EE EE EE EE EE 1 1 1 Introduction of PCR t
11. exported gt B zhs b gill Computer P ch Network gt IEB Control Panel a Recycle Bin GeneTouchSetup 20110809 newpcr110627 5 4 3 Equipment management Include connection operation connection and disconnection and instrument management searching for instrument adding instrument editing instrument removing instrument and showing all instruments Connect Disconnect All instrument Q amp 4 4 Search Add Edit Remove instrument instrument instrument inetrument Connect operation Instrument manage 26 Instruction Manual of GeneMax Thermal Cycler 1 Connect Select equipment name sub item in the equipment list and click connect amp 4 or right click connect equipment in the menu lt will show in connection show in disconnection 2 Disconnect Select equipment name sub item in the equipment list and click disconnect If the file or system parameters in the equipment are being edited then the dialog box will pop up prompt for save 3 Search for instrument ot Click search for instrument then a dialog box like the right figure will appear Search instrument rd 192 168 0 1 192 168 O 255 C Serial port Select added equipment and input equipment name Click OK to save Set instrument name If the instrument needs to be connected please select Solel eae aa ne hee re i SerialNo Communication Address automatically connect SN20110424 TC
12. impaired Hangzhou Bioer Technology Co Ltd shall be irresponsible for the consequence resulted from user s failure to observe the following requirements Caution This instrument conforms to Class I and Protection Class IP20 of GB4793 1 Standard and it is for indoor use This product has been tested to the requirements of CAN CSA C22 2 No 61010 1 second edition including Amendment 1 or a later version of the same standard incorporating the same level of testing requirements a Instrument earth In order to avoid electrical shock accident the input power wire of instrument shall be reliably earthed This instrument uses 3 core earth plug and this plug is provided with a third earth leg is merely used for earth type power socket and is a safety unit If the plug is impossible to insert into the socket the socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall not disassemble instrument without permission Only qualified professionals are allowed to replace elements or adjust the machine inside Replacement of elements under energizing state shall be prohibited c Use of power supply Before turning on AC power make the power voltage consistent with the instrument required one a 10 deviation is allowed Make the rated load for power socket not below instrument s maximum load d Power wire Generally this instrumen
13. name Password Password confirm AnEAABNENNEE rofwfefelr fy el ole os afsfofrfefnfefafe sm 2Pfefvtefefufee ta Save Run Back Main Save interface of PCR program The user can directly select the existing file amp under the user name by clicking icon as shown in the right figure 4 2 Running PCR program 4 2 1 Run new PCR program User name User select File name Password Password conf 1 After saving the new PCR program click run to enter parameter setting as shown below The user can select module or B and alter temperature control mode increased amount of fluid and temperature of hot lid including opening closing hot lid function Ot Run winzard Va mY lt g gt User Nome File Nome ae Verti fom S el7 8 orm 9 0 Sample Type Control Mode Sample Volume Hotlid Control Back Parameter setting interface ta Main Instruction Manual of GeneMax Thermal Cycler The mode Block only takes into account of the temperature control in rise and fall of the module Since the rise and fall of the reagent lags behind the module to certain extent the actual temperature change process of the reagent is different from the program we set The actual time during which the reagent reaches the setting temperature will be far less than the setting time see the relationship between module temperature and reagent temper
14. setting to enter date amp time setting EEE interface Click OK to save after setting Date 2011 11 18 yy mm dd Time hh mm ss 1 219415 017 98 Date amp time setting interface 4 3 2 Language Click language on the main menu Langvage of system setting to enter language setting interface The instrument has integrated two languages namely Chinese and English Click OK to save after setting English Chinese Language setting interface 4 3 3 Backlight adjustment Click backlight adjustment on the main menu of system setting to enter backlight adjustment setting interface Press the navigation bar to move right and left to adjust backlight brightness of the instrument Click OK to save after setting Backlight adjustment setting interface 4 3 4 Hot lid setting Click hot lid setting L on the main menu of system setting to enter hotlid setting interface The first is when the module temperature below a certain value need to shut down hotlid prevent hotlid temperature on the influence of the temperature of module The second is when cover heating modules need to keep a constant temperature untill hotlid temperature up to hotlid set temperature Click OK to save after setting ot lid setting interface 4 3 5 Tone Click tone on the main menu of system setting to enter tone setting interface Click OK to save after setting Tone setting interface
15. to ordinary computer by RJ45 standard interface which is easy for software upgrading 1 4 Use of GeneMax Thermal Cycler Integrating a number of advanced technologies GeneMax Thermal Cycler meets the demand of different PCR experiments and ranks top in similar products GeneMax Thermal Cycler is attached with 1 2 J 4 0 2 ml standard centrifugal tube Strip of 8 tube Strip of 12 tube Full half apron plates er rl GEE SNA Instruction Manual of GeneMax Thermal Cycler CHAPTER II FEATURES This chapter introduces the usage transportation storage conditions of the instrument as well as its basic parameters performance and functions 2 1 Normal working conditions Ambient temperature 10 C 30 C Relative humidity lt 70 Power supply 100 240V 50 60HZ Caution Before using the instrument please make sure the working condition meets the above requirements Especially the power line must be reliably earthed 2 2 Transportation and storage conditions Ambient temperature 20 C 55 C Relative humidity lt 80 2 3 Basic parameters Product name GeneMax Product No BYQ6067 Model TC S 96G TC S 384G Module model B 96G B 384G 96 holes x0 2ml 96 holes full apron plates 12x8 test tube 348 holes x0 1ml Sample size bar half apron plates no apron 384 holes full apron plates general Color Max screen 10 4 260 000 4 l LCD display X Color Max screen 10 4 260 000 Colors Colors Temp
16. 0 figure set temperature at 40 C o and temperature gradient at 20 C oO ii Each hole temperature gradient of sda the module do not distribute evenly z 92 Temperature difference of each p 50 hole in the middle of the module io will be larger than that of holes 2 on the two sides of the module 44 Therefore when operating gradient io module temperature of each hole shall be subject to the temperature displayed on the instrument 1 2 7 4 5 6 T 8 9 10 11 12 Column left to right CHAPTER V CONNECT TO COMPUTER 5 1 System configuration Operating system Windows XP Windows Vista Windows 7 System environment Operating environment Net Framework2 0 Other software PDF reader CPU Pentium R 4 Minimum configuration Memory 1GB Hard disc 10GB 5 2 Install software 5 2 1 Install PC software Click ThermalCyclerSetup exe in CD ROM to install Gene Max online program 30pcs GeneMax instruments can be controlled simultaneously by connecting to computer program GG 5 Setup ThermalCycler Welcome to the ThermalCyder Setup Wizard 1 Click and run ThermalCycler setup ae This will install ThermalCyder version 1 0 on your computer It is recommended that you dose all other applications before exe to select installation language and continuing Click Next to continue or Cancel to exit Setup click OK ig Setup ThermalCycler Select Destination Location Where should ThermalCy
17. 3 69 1 70 0 54 3 55 2 56 1 57 0 57 8587159 016041613622630 63 9 64 8 65 7 66 5 67 4 Close 28 Instruction Manual of GeneMax Thermal Cycler 2 System help Click help then the help file will appear 3 Software edition Click about the system dialog box will pop up and the software edition can be referred to 5 4 5 Run 1 Run file Open the file and click run then a run file dialog box like the right figure will pop up Select instrument Do you want to run file Please select instrument or module and input parameters module test tube type amount of increased fluid instumem modue gube Tube contol Hatitemp EE temperature control mode hot lid temperature and mk oca CA fom 20 OM 105 OB 05m A 20 tube 105 running method limited to Verit Module Click OK to run program Select all Unselect all After running the file the running status on the Online Offline right of the main menu shows like the right figure EE MEEN GeneTouch 48D Module temp 27 3 C B Module temp 29 9 C be Details of instrument operating status are as follows oe ay Bet Tap OPC Bet Tang MPC Bal Time 00 00 Fet Time 40 00 rad i D Brad 2 Browse running history Right click system history and a history table as follows will pop up Module Username File name Begin time End time State name
18. CPU 40P J6 ZJB 34P HL EE OF OLA IST LINE COSEL HJE h ERA 36V600W EG ER J lle a iZNLET4 aesnennnen FH Ra A l ACIDC1 EE FUSE1 seGG10 2 100 240V 50 60Hz i 1 300mm R13 33A MR 4 210 C5 BB i i ADA600F 36 E j LINE2 LINE2 i AC250V8A 5 20mm 1 350mm L aim st Ts ii el H E 1350mm y LINE2 PD 160mm V V Vi v Ee Z yu FUSE FUSE v j LINE2 RAAR IES in we PE RF187 8 F cee on FUSE2 3 74271222 DIE N f LINE2 a 7 AC250V8A 5 20mm i FR EER UTAX AS Al AC100 240vt BEE 300mm HEFE GES Le FEE ME ND SH BA FA Sig 2 BLE JO eh ye ea Ae Be Hh 55 MEMORANDUM 36 Add Tel Fax E mail P C r di BIOER slo TECHNOLOGY 1192 Bin An Rd Hi tech binjiang District Hangzhou 0571 8777 4307 or 010 8225 3949 beijing 0571 8777 4553 instrument bioer com cn 310053
19. P 192 168 0 68 SN20110422 SerialPort COM3 SN20110423 SerialPort COMS Selectall Unselectall V Automatic connection 4 Add instrument Click add instrument a and a dialog box like the right figure will appear Input instrument name and Add instrument address Click OK to add instrument The instrument name shall not be empty and the max Instrument name bytes shall be 12 Only numbers and letters are allowed in the instrument pi i se name Instrument address The same instrument name is not allowed The instrument address shall not be empty and the same address is not allowed to be used in different instruments 5 Edit instrument Select equipment name sub item in the equipment list and click edit instrument An equipment edition dialog box like the right figure will pop up input instrument name and instrument address When connecting the instrument the instrument address cannot be edited 6 Remove instrument Select equipment name sub item in the equipment list and click remove instrument The instrument cannot be removed when it is connected 7 Show all instruments Click all instruments and a list of all instruments will appear to show all instruments information 8 Instrument system information Select system setting a setting dialog box like the right figure will appear Instrument system information contains beep alarm key sound completion sound te
20. ature in the right figure Temperature C Tube mode takes into account of the backward process during which the rise and fall of the reagent lags behind the module After reaching the setting temperature there will be an overshooting process so that the reagent can reach the setting temperature at a quicker speed In the setting time that similar to that of Block mode the actual time during which the reagent achieves the setting temperature evidently prolongs see the relationship between module temperature and reagent temperature in the right figure Temperature C 2 After setting temperature control mode increased amount of fluid and temperature of hot lid click OK to run PCR program Seg Lid Temp Block Temp Remain Time Cycler 22 99 IS OL 138 0e 35 50 Hotid heat Lid Temp G IG Block Temp 10 0 A Stop Pause Next Main Program running interface In the running process the user can click main menu to return to the main menu or carry out other operations Such as creating new file If you want to return to the program running interface again you can click the running interface at the top of the screen on the main menu Seg Cycler Remain Time De 35 50 Lid Temp 105 0 C Block Temp BIOER Ver 1 02 BIGER E Run 4 2 2 Run saved PCR program J Recent it Share Hi MH ss 9
21. avsavsavsevsersersersersessensersensensensensenvenvenvsnsssvssvsevsensensensensensensensensensensensensenvenvenvsevsensevsensensensensensensensens 10 215 SAS EE ER EE EE EE naive 11 4 2 Running PCR ode TEN 12 4 2 1 Run new PCR 0110 0 lfe LA A M 12 4 2 2 Run saved PCR 0110 0 IE Rieti ReatE SE SEOS SESE SEES SOSESSIOSSOSSESSSOSSOSSOSSOOSSOSSOSSOOSSOSSOSSOOSSOSSOSSOOSSOSSOSSOOSSOSSOSSOOSSOSSOSSSOSSOSSOSSOOSSOSSOSSOOSSOSOSSSOSEOSSOSSOOSEOS 14 4 3 Set system parameters SS 15 221 Dee ST PG EE EE EE G ekke 15 EET ET KAR ES EE Essensen essensen 15 4 3 3 Backlight GR TA sende 16 EYE RAN EE EE Essensen 16 A35TONG reer eeeeeeEERRR RR RR eeEEERRRRRRRRReeeEERRRRRRRRRReeeERRRRRRRKRReeeeERRRRRRRKKReeeERRRRRRRKKReeeeRRRRRRRKKReeeeeRRRRRRRKKReeeERRRRRRRKKReeeERRRRRRRKKReeeeRRRRRRRKKReeeeeRRRRRRRRKeeeeee 16 4 3 6 Screen calibratiOn eer RR sEEEERRRRR RR eeEERRRRRRRRReeeeERRRRRRRRKReeeEERRRRRRRKReeeeRRRRRRRKKReeeERRRRRRRRKRReeeERRRRRRRKKReeeERRRRRRRRKReeeeeRRRRRRRKReeeeee 16 A37USB eertse eeEERRRRRRRRRgeeEEERRRRRR RR eERRRRRRRKRReeeeERRRRRRRKReeeeERRRRRRRKKReeeERRRRRRRRRReeeeRRRRRRRKKReeeeeRRRRRRKRRReeeERRRRRRRRRReeeERRRRRRRKKReeeERRRRRRRRRReeeeRRRRRRRKKeeeeeeeRR 17 4 4 Distribution characteristics of gradient module eee R ekke Reese esse seer 17 CHAPTER V CONNECT TO COMPUTER ttssttreermenmeenmsenseersesrsesssestaesrsnssrssrsseeseeseessesesesesesrsesnsessesnsnen 18 RE PP AE LE EE aneavneanestaoaneanatat 18 5 2 Install software teeeeeseesssssssssssssssssseeesseeesssssss
22. blic Location Contact Click the Modify check box to modify configuration JA OK x Cancel IP address setting 2 Click Operating Mode and select Modify Click Settings and operating mode will pop for selection Select TCP Server Mode and select K Operating Mode setting 3 Click Serial option and set in the Serial Settings window that pops The specific parameters are as follows Click OK after finishing setting Serial Setting 4 Click OK to finish Configuration setting 4 Click COM Mapping and Add target to add target instrument Carry out COM port settings in the pop up dialog box Click OK after finishing Click Apply Change l to save altered setting COM port settings Operating Hode 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Real COM Mode TCP Server Mode TEE Server TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout 0 99 min Inactivity Timeout Max Connection o o 0 65535 ms Data Packing Optional Delimiter 1 00 O ff Hex Delimiter 2 00 0 ff Hex Force Tx Timeout 0 0 65535 ms JA OK X Cancel Serial Settings 1 Portis Selected 1st post is Port 1 COM Fort Settings Port Humber 1 Parts Selected Tt port is Pot 1 Basic Settings Advanced Settings Serial Parameters
23. cally after power off Caution The above mentioned software function is merely for reference The software function would be modified without notice WA Instruction Manual of GeneMax Thermal Cycler CHAPTER III PREPARATION This chapter mainly introduces structure of the instrument and preparation before startup When using the instrument for the first time read the contents in this chapter carefully before startup 3 1 Structure diagram Hot lid Max screen USB interface Installation method Take the Max pen out of the accessory and strip the 3M rubber release layer at the back of the pen buckle after determining the fix location Then paste it on the fixed location as shown Caution The fixed location can be adjusted according to your need When fixing the location must be determined Then strip the pen buckle off from the back 3M rubber release layer and fix it on the instrument Module Ventilation hole ee IM M r RJ45 interface je ty in Power switch u Ui Mm i l Power socket Thy lin with fuse Unn Normal version Module Ventilation hole ning Un i Mo RJ45 interface Power switch Power socket with fuse Communication interface Senior version Caution The fuse specification must be 250V 10A 5 20 Use qualified product to replace the fuse Contact 3 2 Examination before startup Please confirm the following contents before plugging in for
24. cler be installed Ld Setup will install ThermalCycler into the Following Folder 2 Se ect N Stal at O N O cat O nN of th e To continue click Next IF you would like to select a different Folder click Browse software and click next At least 2 7 MB of free disk space is required 18 Setup ThermalCycler Select Start Menu Folder Where should Setup place the program s shortcuts Setup will create the program s shortcuts in the Following Start Menu Folder To continue click Next IF you would like to select a different folder click Browse 3 Click next Instruction Manual of GeneMax Thermal Cycler 5 Setup ThermalCycler Ready to Install Setup is now ready to begin installing ThermalCycler on your computer Click Install to continue with the installation or click Back if you want to review or 4 Glick install change any settings Destination location C Bioer Thermalcycler Start Menu Folder ThermalCycler Cancel Heg Setup ThermalCycler Completing the ThermalCycler Setup Wizard Setup has finished installing ThermalCycler on your computer The application may be launched by selecting the installed 5 Finish installation Click finish or Click Finish to exit Setup 5 2 2 Install tool software jo Felep Hat erk Enabler dimins air len Welcome to the Network Enabler Administrator Setup Wizar
25. d Tru voll rota Hete E rakke Acetate 211 on var Compara Fic meme that you der all other pp sr bel 1 Click and run nead_ setup exe cortnuang Diek Hard Ba riras of Cancel Ba gal Spt is Setup Network Enabler Administrator Select Destination Location Where should Network Enabler Administrator be installed CJ Setup will install Network Enabler Administrator into the following folder To continue click Next If vou would like to select a different folder click Browse 2 Click Next C Program Files Network Enabler Administrator At least 2 5 MB of free disk space is required 15 Setup Network Enabler Administrator Select Additional Tasks Which additional tasks should be performed Select the additional tasks vou would like Setup to perform while installing Network 3 Select installation list and click Next Enabler Administrator then click Next Additional icons 5 Setup Network Enabler Administrator Installing Please wait while Setup installs Network Enabler Administrator on your computer T Extracting files 4 G IC K n stal C Program Files Network Enabler Administrator NEADMIN exe PEELE ELLE ELEELELELEELELLELELELELE LLL 2 Setup Network Enabler Administrator Completing the Network Enabler Administrator Setup Wizard os ur J Setup has finished installing Network Enabler Administrat
26. ddress IP Address Configuration dig COM Mapping Monitor Port Monitor Qi IP Address Report Message Log 0 Monitor Log 0 No Time Description o Now 2008 1 3 14 15 25 GI EE fea N Network Enabler Administrator Configuration E lalz File Function Configuration View Help 22a2 LR Function Configuration 2 Network Enabler Module s 5 A Network Enabler Admit No Model MAC Address IP Address Configuration 1 NE 41105 00 30 8 14 60 78 1921680154 df COM Mapping 2 NE 41105 00 30 8 18 02 10 192 168 0 103 Monitor Port Monitor Q IP Address Report Found Network Enabler Modulef s 2 COM Port Deleted 192 168 0 154 Port1 COM2 COM Port Deleted 192 168 0 103 Port1 COM4 COM Port Added 192 168 0 154 Port COM3 COM Port Added 132 168 0 103 Port1 COMS 2011 4 13 14 32 14 2011 4 13 14 32 43 2011 4 13 14 32 43 2011 4 13 14 32 43 2011 4 13 14 32 43 Now 2011 4 13 14 45 36 Configuration Information Auto Warming IP Address Report Password DigitallO SerialCMD sly Basic Network Seral Operating Mode AccessblelPs NE 41105 Modify MAC Address 192 168 0 245 00 90 E9 10 E2 B7 IP Address Serial Number 3898 Netmask 255 255 255 0 Gateway Firmware Ver Ver 4 0 IP Configuration Static DNS Server 1 BIOS Ver Ver2 1 DNS Server 2 Status Modify Data Mode v Enable SNMP Community Name pu
27. e increment per cycle EN ENN EN EN NERE Ext temp Ext time Rate Step All view Rate ED f2Jsjefsfefrlel ee slee for om Exttemp Exttime Rate Step All view 72 0 72 0 72 0 72 0 72 0 72 0 Normal O Advance co OO UI BR UNBE DE Cancel Speed heating and cooling speed Step step temperature is the temperature difference between two adjacent blocks range 6C 6 C Click Normal to set the same temperature difference between 6 blocks Ext temp i Exttemp Ext time Rate Step All view 0 Normal 09 40 0940 0940 09 40 09 40 09 40 L K Click Advance to set separately the temperature Click Graph you can see every block temperature in differences between these 6 blocks the graph Advance co 1 2 3 4 5 6 Instruction Manual of GeneMax Thermal Cycler Display all browse the current setting of extended parameters Ext time Rate Step All view Exttemp 0 0 Exttime 00m00s Rate 4 0 s Step lt and means to move the cursor left or right means to alter negative and p ails n positive data 4 ue M hal Clr means to clear data Seg Goto Delete OPT Back Save run 4 1 5 Save Run After setting PCR program click save run to enter save interface as shown in the right figure The user can input user name and file name in the interface and also can adopt password assess mechanism for the data Finally clicks save 1G User name File
28. echnology ee xxx 1 1 2 Example of PCR technology application SCOPE reer RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR BEREG 1 1 3 Features of GeneMax Thermal Cycler EE NN NE EE EE D 1 4 Use of GeneMax Thermal Cycler EEE NERE EE EE NEE EE secre D CHAPTER II FEATURES eeeeeeeeeeeeeteeseeeeeeeeeeeeeeenesssssssssssseeeeeeeeneeeesssssssssssssseeeeeeeeeenssssssssssssseeeeesenenennsssssssssssseseeeennnnensssssssssssssssene 3 2 1 Normal working conditiOns hv V V 534VV vvv5vv4vv4v 54444554 3 2 2 Transportation and storage conditions ees 3 2 3 Basic parameters 544 544444 444LS4L4LL44444444444 44444 EE 3 24 Software functions EE EE N EE N N E AE E 4 CHAPTER III PREPARATION EE EE EE EE OE 5 3 1 Structure ELE EE EG 5 3 2 Examination before startUID J ZZsc v H 6 33 Startup ER REE ENE EEE EE Oe 6 3 4 Installation of module unit ETE NE 6 3 4 1 Installation procedures EE EE EEE EE 6 342 Dismantling procedures HR 6 CHAPTER IV OPERATION GUIDELINE jereeseereereerevenenseeseesersersersessansevensessessessessessessassessssessersersessessessevsesessessersersenn 7 4 1 Creating PCR program EE 7 4 1 1 Add or delete program SEGMENT vsrrrerrereevsersersersersersessesvssvsevsersessessesssssenvssvsevsersensensensenssnvsevsevsensensensesssnvsnvsevsensensensensensesssevsensensensensnn 8 A D Program and constant temperature GER EE OR EL AE EE OE EE OE EE RE 8 4 1 3 Cycle R EE EN EN EE RER ER 9 4 1 4 Extended parameters vvvvvvverserseaseavease
29. ecific and sensitive technology has been widely applied to life science areas at astonishing speed Remarkable achievements have been witnessed in cytology virology oncology genetic disease forensic medicine animal plant immunology and etc It is a milestone in contemporary molecular biology development 1 2 Example of PCR technology application scope Research Clinical diagnosis Immunology Human genome project Forensic medicine Oncology Histology population biology Paleontology Botany zoology Gene clone dna sequencing mutation analysis gene recombination and fusion protein regulating dna sequence identification transposition insertion site mapping genetic modification detection synthetic gene construction clone construction carrier expression certain gene s incision enzyme diversity detection and etc Bacteria spirochete mycoplasma chlamydia mycobacterium rickettsia diphtheria bacillus pathogenic e Coli dysentery bacillus aeromonas hydrophilic and c Difficile and etc Virus htlv hiv hbv hev hpvs ev cmv ebv hsv measles virus rotavirus parvovirus b19 and etc Parasite malaria and etc Human genetic disease lesh nyhan syndrome mediterranean anemia hemophilia bmd dmd cystic fibrosis and etc HLA typing T cell receptor or antibody diversity qualification autoimmune disease gene mapping lymphokine quantification and etc DNA mark generated from scatter repetiti
30. eeseesssssssssssssssssseereesessnnsssssssssssnn 30 Bo COCO OE RE E EA E 30 6 2 Network Enable Administrator setting RR RR RR RR RR RR RR RR RR RR RR RR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 30 6 3 Software upgrading RR RR R RR RR RR RR RR RR RR RR RR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 39 CHAPTER VI FAILURE ANALYSIS AND TROUBLESHOOTING reer eers esse esse seek sees Reese Reese se ee 33 7 1 Failure Analysis and Troubleshooting HuuuueeeeeeeeeERRRRRRRR EER E EE EE egg RR RRRRRRRRRRRRRRRRRRRRRR EER eeeeeeeeRRRRRRRRRRRRRRRRRRRRRRE EE eeeeee 33 Appendix Wiring Diagram ER EE EE EE EE NN EO N Kea EN 35 Instruction Manual of GeneMax Thermal Cycler CHAPTER I INTRODUCTION The contents in this chapter include introduction of PCR technology examples of PCR application introduction of Thermal Cycler and features of GeneMax Thermal Cycler 1 1 Introduction of PCR technology Polymerase chain reaction PCR is a nucleic acid amplification that simulates natural DNA replication in vitro It takes 2 DNA chains to be amplified as template uses a pair of artificially synthesized oligonucleotide primer as mediator and amplifies certain DNA fragment quickly and specifically by thermostable DNA polymerase Ever since PCR technology was invented by Mullis in 1985 and thermostable DNA polymerase was discovered by Erlich in 1988 this simple fast sp
31. erature range 4 C 105 C 4 C 105 C Max cooling rate sae Is 2 80 s Temperature uniformity 0 250 lt 0 250 Temperature control precision 0 3 C sel Temperature fluctuation lt 0 10 lt 0 1 C Gradient temperature range 30 C 105 C 30 C 1050 Gradient temperature range 1 30 C 1 30 C Temperature range of hot lid 30 110C 30 110C File saving amount gt 250 gt 250 Energy consumption GOOW GOOW Operation mode Continuous running mode Continuous running mode Dimensions 454x284x312mm 454x284x312mm Fuse 250V 10A P5x20 250V 10A P5x20 PC interface RJ45 RJ45 Net weight 15 kg 15 kg 2 4 Software functions a Edit the file including file number temperature and time of each segment cycle times setting file view revision and deletion automatic file connection gradient temperature setting the maximum paragraph step and cycle times for editable file are 10 100 and 99 respectively The scope for gradient temperature setting is 1 C 30 C b Save the file c Revise the file adjust the rate for temperature rise fall refine temperature and time for cycling automatically and display and calibrate the time d Display the temperature time cycle times setting and real time value during the file running including sound prompt total rest time estimation for file running failure protection and alarming e Stop the file running including pause the file running stop the file running recover automati
32. ers Basic Settings Advanced Settings Serial Parameters re Aure aud Rate 38400 RTS CTS Parity None FIFO Enable Tx Mode X Data Bits 8 z Interface R 232 di COM Number COM3 curent assigned Stop Bits 1 Enable v Fast Flush Only Flush Local Buffer Jf OK X Cancel Apply All Selected Parts COM port setting v OK X Cance 6 3 Software upgrading First continuously press the Max screen and Firavare upgrade i Upgrade bin file turn on ine instrument power Starting up to enter SEER SERE upgrading status Upgrade target Then run PC software ThermalCycler Click tool ae firmware upgrading to select lower computer Vag a software bin that needs in upgrading God meen mathod Serial Port Select upgraded object and communication mode old and instrument address Communication mode and instrument address press 6 2 Network Enable Administrator setting configuration Settings and click upgrading to start upgrading After finishing upgrading the equipment can be normally operated after restarting 33 Instruction Manual of GeneMax Thermal Cycler CHAPTER VII FAILURE ANALYSIS AND TROUBLESHOOTING This chapter mainly introduces possible failures of this instrument and reason analysis as well as troubleshooting 7 1 Failure Analysis and Troubleshooting S N Failure Reason Analysis Troubleshooting Power supply is not connected Check the power supply and co
33. g interface of PCR program 1 00 30 Temp and time set Temp g Time 00 30 mm ss else le vee ef ef fe sur gt we TT maa Z Goto Delete OPT Back Save run Edit program segment Instruction Manual of GeneMax Thermal Cycler 4 1 3 Cycle function On the setting interface of PCR program first click the program segment that needs to add cycle function Then click jump ve to enter the setting interface of cycle function as shown in the right figure Jump setting Seg 01 94 0 00 30 Repeat Times REY Seg 02 94 0 00 30 Seg 03 62 002 05 Seg 04 72 0 00 20 Seg 05 72 001 00 Seg 06 04 0 e N gt 4 te 0 hul Seg Goto Delete OPT Back Save run The jump function takes effect only when it jumps from the later program segment to the former one See as the above figure seg05 seg03 In setting you need to enter the program setting status of seg05 then click jump to set cycle function O amp ul Seg Goto Delete OPT Back Save run Finished jump setting 4 1 4 Extended parameters You can enter extended temperature Tl extended time speed and gradient and other operations by clicking extended parameters Ext temp Ext time Rate Step All view Exttemp OD Ext temp Ext time Rate Step All view Exttime CO 1fe sja sje r e efofef J for oa else 7 ler o Zor Extended temperature temperature Extended time time increment per cycl
34. mperature keep sound and hot lid setting the lowest temperature of hot lid and module retention temperature Clicks save after finishing setting The lowest hot lid temperature range OC 99 9 C Module retention temperature range OC 30 C The system information cannot be set when the instrument is disconnected When there is only single module in the connected instrument the display area of module B cannot be altered 5 4 4 Other management 1 Temperature gradient Click temperature gradient in the menu bar and a gradient dialog box as follows will pop up Temperature gradient range temperature OC 690 C Gradient temperature 00 C 300 C Edit instrument Instrument address 192 168 0 47 HSE x Sound Control Beep alarm when run finish Key been Complete ON v Module Lowest hotlid temperature C Hotlid will close when temperature lowes this A Module keep temperature 20 me Module keep this temperature when hotlid temperature is rising up Module B TE O Es Hotlid will close when temperature lowes this Lowest hotlid temperature A Module keep temperature 10 Ce Module keep this temperature when hotlid temperature is rising up Gradient temperature Temperature 50 Gradient 20 gt 13 14 15 16 17 18 19 20 21 22 23 24 1 2 3 45 B 7 8 98 10 14112 Q0000000000 10000000 00000008889 00000000 50 0 509 51 7 52 6 53 5 68
35. nnect 1 After the power supply switch is turned on Fuse is burnt Replace fuse 250V BA P5x20 the display screen is not on and the toot sound is not heard either Switch is damaged Replace switch Others Contact the supplier or the manufacturer During file running process there is prompt Module sensor is damaged or in poor contact Heat sink sensor is damaged or in Contact the supplier or the 2 of Please Contact the Manufacturer poor contact manufacturer Hot lid sensor is damaged or in poor contact The ventilation hole is blocked Clear obstructions at the vent 3 Obvious changes in module heating rate Open the case and check the wires or inaccurate temperature control Temperature cooling rate of the module 4 obviously slows down or or it cannot slow down under room temperature Wires get loose Cooling chip is damaged The ambient temperature or humidity is So high that they exceed application conditions of the instrument Tighten the screws of wire terminals if they get loose Contact the supplier or the manufacturer Please properly adjust the ambient temperature and humidity Cooling chip is damaged Fan is damaged or out of work Contact the supplier or the manufacturer The temperature sensor is damaged Contact the supplier or the 5 The module neither heats nor cools AE pP Cooling chips are all damaged In system parameters setting interface Set the ho
36. of Computer s test Enter new user name The user name can contain only letters and numbers 3 Delete user Select the user needing to be deleted in the equipment list and click delete or right click delete user to delete The program file under the user name will be simultaneously deleted when deleting the user 4 Copy Select the user needing to be copied in the equipment list and click copy right click copy user in the menu to copy the user and the program file under the user name It cannot be copied when the program file under the user is being edited 5 Paste Select the user sub item under equipment option in the equipment list or equipment option in the equipment list and click paste 4 or right click paste in the menu to paste the user and the program file under the user name If the copied user has already existed it will prompt whether to cover when pasting If the program file under the existing user name is in use then pasting cannot be carried out Pasting may be finished by dragging mouse 6 Import user Browse For Folder 0 Click import amp to select the folder saved by the user in the pop up dialog box Click OK to import user If the user selected has no program file then user cannot be Please select import user HA Desktop imported b Sql Libraries If there is same user name then it will prompt whether to cover in gt UB zhs
37. or on 5 Fi nN l S h l N stal lat O N C IC k Fi N S h f your computer The application may be launched by selecting the installed icons Click Finish to exit Setup 5 3 Online setting 5 3 1 Connect to PC Connect the equipment with the computer through network cable intersect connection B Network cable mad i Ca j Computer Instrument 21 Instruction Manual of GeneMax Thermal Cycler 1 Run Network Enable Administrator tool software 2 Click search button e or right click Broadcast Search option in Configuration the tool will automatically search for the equipment 3 Double click the network number searched in list column to enter configuration setting page as follows 1 Click Network option and select Modify Input IP address and subnet mask The IP address of the instrument shall be in the same network segment with that of computer For example if the computer IP is 192 168 0 1 then the instrument IP shall be set as 192 168 0 XXX Among them the range of XXX is 2 254 and cannot be the same with computer IP Network Enabler Administrator Configuration Sele File Function Configuration View Help 2a2 2en Function Configuration 0 Network Enabler Module s YAY Network Enabler Admi No Model MAC A
38. powering on 1 Whether the power supply conforms to the required voltage see power supply requirements in Chapter II of the file 2 Ensure the plug of power cord has been reliably plugged in the socket 3 The power cord is reliably grounded 3 3 Startup Turn on the power switch turn the switch at The Thermal Cycler will produce one toot sound It indicates that it is powered on At that time the screen is still black Please wait for about 10 20 seconds The main menu will then appear Now you can edit browse alter and delete PCR Thermal Cycler file as well as other operations Instruction Manual of GeneMax Thermal Cycler CHAPTER IV OPERATION GUIDELINE This chapter provides detailed introductions of the methods for the edition browsing alteration and deletion of the PCR THERMAL CYCLER file its running as well as setting of instrument system parameters etc Caution If sound occurs or the display is abnormal or failure alarming and tone occurs during the process of the self test of the instrument after the instrument startup please cut off the power supply immediately and contact the supplier Caution If the sample quantity in your experiment is less than the number of taper holes of the module please try to distribute the sample test tubes evenly when placing tubes so as to ensure that the hot lid can be stably pressed on top of the test tube when running the file Meanwhile make the load of the module even to guaran
39. racteristics including heating and cooling rate stability and volatility and due to the uncertainty of biological experiment and external vulnerability successful PCR program effect in one instrument may not apply to another Therefore when you change the instrument please adjust PCR running program so as to achieve the ideal state 34 ICE t to change without further not is subjec For reference only and iagram D iring W Instruction Manual of GeneMax Thermal Cycler Bi Appendix 1 Ban DERF BYQ507101310000 100mm 47 ps Hail EE abs LINES Al on LL G te k EG BA PCB2 Exi BYQ507101410000 PP i eN mm ma sponsae H J7 CPU40P EZTEN ARE BE J2 4 TE2 2 13 sv J9 1 LAN J5 4 TE2 i J2 14 GND J9 2 LAN MiGenePro amp Hj sr ER PCB4 J4 1_V i J9 3 LAN ANSER ETEN J4 2 V J9 4 LAN J4_BLOCK 48P BYD507101320000 110mm i J1 2 RxDO J9 5 LAN BYO507102000000HEHR SW1 MEE ee pe J1 3_TxDO J9 6_LAN ok HE AS BLE i HA El i AG EUR FU AYES SS RE TET ag ar roes F U5 a 5 CPU K TOLV i E04SR150718 Ali LINE4 150mm J2 1 SW En J26 BLK amp ZJB 40P J PLUS1 1 J2 2 SW FANT VAD J PLUS1 2 J PLUS1 3 J2 3 SW AD1224HB F51 press J3 2 ON OFF J PLUS1 4 J3 1 SW 7 7 I J24 POWBOARD 40P J6 1 USB VCC J PLUS1 5 J3 2 SW DC J6 2 USB DM1 ee RG ony Fre J3 1 HV LINE3 DC N i J J6 5_USB GND J6 6_USB GND J23_GND J5
40. sssssssssssrrreresesssssssssssssssssssseresssenessssssssssssssssssersssssnnsnsssssssssssssssseresssennnssssssssssssssssssssse 18 5924 Install PC Software meeeesssssssssssssssereeesesssssssssssssseeereesstsssssssssssssssrreessenssssssssssssssseressennssssssssssssssseessssnnnssssssssssssseressssnssssssssssssssssssse 18 529 Install tool software ersssvsssrssssseeeeseessssssssssssssrerresesessssssssssssssserressenssssssssssssssseressnnnsssssssssssssseesssnnnnssssssssssssseeesessenssssssssssssssssssn 19 5 3 Online setting EE 20 5 3 1 Connect to PC EEE OO EE ET RI OE EA EE TE EO EO SP OR 20 ENN OR EE ER OE N 23 5 4 1 User management ee RR RR RR RR RR RR RR RR RR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RR 23 5 4 2 File management tstssssssssssssrsssssssssssssssssssssssssssssssssesssessssesseseseseseeereeeeeseeereeereeseeeseeeseeseeeeeeeeseeeseseeseeseeeseseeesesseeseeseeeeeseeeeeeessesssesseesssssssenn 24 5 4 3 Mo AE DE er N DAN eee sees esse eee eeeeeeeeeeeseeeeee seere sees esse ee eeee sees eee eeeeeeeee sees eeseeeeeeeeeeeeeeeeeeeseeseseesesessesesene 26 5 4 4 Other management ee RR RR RR RR RR RR RR RR RR RR RR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RR 28 TAAR AE E EA E E aden encoun nceeenem eee 29 CHAPTER VI FIRMWARE UPGRADING ssesstseesseseeeeeseasssssssssssssssseeeresessesssssssssssssssser
41. t lid status at a certain the hot lid status is set as OFF temperature value Temperature control in file edition 6 Hot lid cannot heat interface is set as so that the Set the temperature time as number form hot lid automatically closes Connectors get loose pe SEP EL ME AOIG AG Contact the supplier or the manufacturer damaged Temperature sensor of the hot lid is damaged 7 Chip is in poor connection The screen shows abnormal characters Chip is broken Contact the supplier or the manufacturer S N Failure Reason Analysis Troubleshooting Have not set hot lid temperature Please refer to 4 3 in Chapter IV of the the hot lid is set as OFF Instruction on Setting Hot Lid Temperature Adjust hole location of the reaction tube 8 Reagent in reaction tube evaporates The reaction tube is unevenly placed try to place in a symmetrical way Please tighten the reaction tube The reaction tube is not tightened before placing it into the instrument NO 2 The extwrnal environment is too low will cause radiator alarm not instrument anomaly Caution 1 The user is not allowed to open the housing of the THERMAL CYCLER for inspection by himself during the warranty period If any failures that require opening the housing for inspection occur the supplier or manufacturer should be contacted timely 2 Since different brands and all instruments have their own temperature control cha
42. t uses accompanied power wire The damaged power wire shall be replaced by one power wire of the same type and the same specification It can t be repaired The power wire shall neither be compressed nor be laid in gathered personnel location e Insertion and withdrawal of power wire At insertion and withdrawal of power wire the plug shall be correctly held with hand At insertion the plug shall be completely and tightly inserted into the socket and at withdrawal heavily pulling the wire shall not be allowed f Placement of instrument This instrument shall be fixed in a low RH and low dust place apart from water source e g water pool and water pipeline and the room shall be well ventilated and free from corosive gas and interterence of strong magnetic field The instrument shall not be placed in a wet or dusty location The openings on this instrument are for ventilation circulation and in order to avoid over heat of the instrument body they shall not be blocked or covered When a single set or several sets of instrument are used the interval between its surrounding ventilation opening and its nearest object shall not be below 50cm or 100cm Also don t use the instrument on loose or soft surface or the air inlet at instrument bottom might be blocked Excessive temperature would impair the test performance or result in instrument failure This instrument shall not be used in location subjected to direct sunshine The instrument shall be kept a
43. tee that the temperature changes of each test tube are uniform Caution Turn the Knob in a counterclockwise way as required in knob figure press tightly in clockwise direction and release in counterclockwise direction before closing the lid until hearing twice clang sound Then turn the knob in a clockwise way before startup Turn another round after hearing twice clang sound Twice clang sound will be heard no matter turning the knob in a counterclockwise or clockwise way Unlock device knob can be released when pressing the unlock device and turning two rounds in a counterclockwise way simultaneously 4 1 Creating PCR program Click new file amp in the main menu enter setting interface of PCR program tay SIG i Nr K Er jS BE ANG ky BIOER Ver 1 02 e B os cp mi Run New File System Seg Goto Delete OPT Back Save run System main menu Setting interface of PCR program 4 1 1 Add or delete program segment You can add or delete program segments by clicking segment or delete T at the bottom of the screen on the setting interface of PCR program o 10 94 0 00 30 inn ai po te 1 ll ve 1 lal Seg Goto Delete OPT Back Save run Seg Goto Delete OPT Back Save run 4 1 2 Segment temperature and constant temperature time You can set temperature of program segment and constant temperature time by clicking temperature or time on the screen on the settin
44. to save If the selected file is not protected by password then it only needs to input new password 4 Rename Select the file that needs to be renamed in the equipment list and click rename It cannot be renamed when the file is opened 5 Delete file PB o S O 4M 6 j Copy Export Create Save peration File operation Computer gt test gt 11 x Segment Add LT Copy FT Delete Cycle 501 502 503 504 Ext Temp 0 0 C Ext Temp 0 0 C Ext Temp 0 0 C Ext Temp 0 0 C Ext Time 00 00 Ext Time 00 00 Ext Time 00 00 Ext Time 00 00 Grad 0 Grad 0 Grad 0 Grad 0 100 C BO 60 40 20 41 4 0 4 0 40 4 0 C A rrr SE oc 00 10 4 165 og 10 4 1 CIS og 10 41 s og 10 Change file password Do you want to change password of Computer gt test gt 11 Enter old password Enter new password The password can contain only letters and numbers Change file name Do you want to change file name of Computer gt test gt 11 Enter new file name Farm The file name can contain only letters and numbers Select the file that needs to be deleted in the equipment list and click delete al If the file is being edited then it cannot be deleted If the file is protected by password then it cannot be deleted unless inputting correct password 6 Copy Select the file that needs to be copied and click
45. ve sequence genetic map construction detect DNA polymorphism sperm drawing physical map construction Sequencing expression map and etc Crime scene specimen analysis hla dqa analysis and etc Pancreas cancer rectal cancer lung cancer thyroid cancer melanin cancer hematological malignant tumor and etc Genetic cluster research evolution research animal protection research ecology environmental science experimental genetics and etc Archeology and museum specimen analysis and etc Animal inflectional disease diagnosis plant pathogen detection and etc 1 3 Features of GeneMax Thermal Cycler GeneMax Thermal Cycler is a PCR instrument with multiple functions and several applications Below are its features Use advanced thermoelectric refrigeration technology from ITI of US and thermoelectric refrigeration elements made under quality management of Japan which are steady and reliable in quality Temperature can be changed as fast as 4 C second The speed to increase or decrease temperature can be set as per need of experiment Several modes for temperature control better compatibility of reagent Large LCD screen user friendly operation interface simple and easy operation Power off protection available programs can continue after power recovery Height and pressure of hot lid can be set for different reaction tubes which effectively prevent the reagent from evaporation and pollution Connect
46. way from hot gas stove and all other heat source If the instrument is to be set aside for a long time the power plug shall be withdrawn and the instrument covered with soft cloth or plastic film to aviod entrance from dust g Cautions during operation During test cares shall be taken to prevent liquid from dropping onto the instrument Caution In any of the following cases immediately cut off the power supply withdraw the power plug from the power socket contact the supplier or ask qualified repairman for treatment Liquid drops into inside of the instrument The instrument is rained or watered The instrument works abnormally especially generates abnormal sound or odor The instrument is dropped or its casing is damaged The function of instrument is obviously changed 3 Instrument label a Label T HANGZHOU BIOER TECHNOLOGY CO LTD HANGZHOU BIOER TECHNOLOGY CO LTD 2 ii Name Gene Max Thermal Cycler ma Name Gene Max Thermal Cycler Model TC S 96 G H b B A Model TC S 96 G H b B B Power 100 240V 50 60HZ 750W Power 100 240V 50 60HZ 750W Fuse AC250V 10A 5x20 Fuse AC250V 10A 5x20 Som class T IP20 Sorn class IF20 SN BYQGOG7 E Date Ee EE Q P SN BYQ6068E Date J b Warning Sign Warning ZIN AN HOT SURFAGE The instrument is adhered with mark HOT SURFACE During operation of instrument or a period immediately after completion of running of a program
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