User`s Manual
Contents
1. current during an electrophoresis run The tracking dye in the loading buffer runs up instead of down Small DNA fragments are not as bright as the large ones A blue band or irregular line is observed at the electrophoresis front during a run The cable connections to the EZ Agarose Electrode Cap or to the power supply may be loose Electrophoresis is running in the opposite direction The power cables were not correctly connected The gel was not run long enough for the small fragments to be uniformly stained Although EZ Agarose gels are compatible with most commonly used loading buffers this band is observed with some loading buffers The band is due to the tracking dye in the loading buffer Solution If buffer level is too low to run the gel add TAE buffer to the desired level The anode buffer tank at the top of the gel contains ethidium bromide Please follow state and local guidelines to dispose of spilled buffer Ensure that the cables are firmly connected to the EZ Agarose Electrode Cap and the power supply If this does not solve the problem check the connection of electrode wires in the EZ Agarose Electrode Cap Check to ensure the connection of each cable to the power supply is correct Run the gel until the tracking dye is at least three quarters down the gel The band will not affect the results because it runs faster than the DNA bands The band can be avoided by using EZ A2. EZ Agarose Electrophoresis System USER MANUAL EZBiolab EZ Agarose User Manual Table of Contents Safety Information eos GRE bim bem v Laufe dU pt ES FUR E IN DIR 2 Product Descriptio Menee OO os 2 Product Contents znssedivaeiusddatdwsrai pde imd UN op et pe 3 Specifications amp Storage Conditions susssse 3 xitelo Mieuda i o 3 Getting Started What You Need iuiecivbeuttsa ten o oak ee PRU SERE FRPR RR MS Ead vit 4 Preparing Your Samples essen 4 Preparing for Electrophoresis Opening the EZ Agarose cassette sssesseeeee 5 Loading Youl Samples sacssscaste iae d usu ei ia SNR MORPHINE RUE VE 6 Installing the EZ Agarose Electrode Cap amp Cables 7 Running the EZ Agarose Gel esee nenne 8 Completing the RU oiectte pa ox aep Drei DR ERE ERR E RR RT 8 Downstream Processing cccsssccesssecesssecesseecceeecseecsceeaaeeeaeseeseseeeseeaneces 9 Disposing of the EZ Agarose Cassette Components 9 Troubleshooting amp Technical Support seeessssss 10 Related Products ioo omitted citatus tubi ttu a bius etshpO Ut ed URDU 11 Warranty amp Liability aio aep Ya Rai Resa Rok vs bu aded eo AUR ER 11 EZ Agarose User Manual Safety Information Always wear protective clothing when performing laboratory experiments Wear gloves and eye protection use all proper sa
3. e caused by solvent corrosion damage caused by improper handling or user alteration nor unsatisfactory performance as a result of conditions beyond our control EZBiolab Inc shall in no event be liable for incidental nor consequential damages including without limitation lost profits loss of income loss of business opportunities loss of use and other related damages however caused nor any damage arising from the incorrect use of the product 10 EZBiolab Inc 1033 3rd Avenue SW Room 215 Carmel IN 46032 USA Email service Qezbiolab com Phone 317 691 9450 FAX 317 663 0721 Website www ezbiolab com For research use only not intended for diagnostic use All trademarks are marks of the EZBiolab Group or its affiliates O 2013 EZBiolab Inc All rights reserved Rev 12 31 13
4. each cable into an appropriate slot in your power supply Running the EZ Agarose Gel Run gels at constant voltage The recommended voltage is 120V for 20 minute run time Voltage should not exceed 150 volts Note The EZ Agarose Gel Cassette has been designed so that small DNA fragments are as brightly stained by the ethidium bromide in the cassette as large fragments To ensure evenly stained bands run the electrophoresis until the tracking dye is at least of length of the gel The EZ Agarose Cap has a window This window can be opened during electrophoresis to observe the progress of the run if the EZ Agarose Gel Cassette is placed on a light box EZ Agarose User Manual Completing the Run When the electrophoresis is completed turn off the power remove the lid and the buffer see section below for disposal information Place the EZ Agarose Gel Cassette ona light box to observe the results of the run The gel can be photographed directly in the tray Downstream Processing To recover bands of interest cut out the bands and recover the DNA by any standard method Disposing of the EZ Agarose Cassette Components Dispose of all components except the EZ Agarose Electrode Cap and color coded cables These can be reused for your next electrophoresis run EZ Agarose User Manual Troubleshooting amp Technical Support Problem Running buffer was accidentally spilled during gel set up There is no
5. fety precautions when handling the gels Please read the Material Safety Data Sheet MSDS for this product prior to use Product Description EZBiolab s EZ Agarose Electrophoresis System is an innovative product designed to simplify electrophoresis The system integrates running buffer with a precast gel to form a ready to use pre fabricated single disposable unit Because the buffer is preloaded and the gel is precast you only need to load your samples to start a gel electrophoresis run The EZ Agarose System eliminates the tedious steps of system assembling buffer preparation and gel casting and can be run using any power supply Compact All components including precast gel amp running buffer are integrated into a single disposable unit Convenient EZ Agarose gels are ready to use just load your samples Fast EZ Agarose gels can be run at lower voltage and electrophoresis is fast Long shelf life Gels are stable for 18 months Compatible EZ Agarose gels are compatible with most common loading buffers and standard blotting methods EZ Agarose User Manual Contents Each package includes 10 gel cassettes Each cassette contains running buffer and an EZ Agarose gel with ethidium bromide sealed with a buffer dam ina disposable tank 1 buffer dam opener Specifications amp Storage Conditions Number of Wells 10 Running Gel 1 agarose 200 bp 10 Kb 2 agarose 100 bp 2 Kb 3 5 agar
6. garose Sample Loading Buffer For technical support contact EZBiolab service ezbiolab com EZ Agarose User Manual Related Products EZ PAGE Products EZ Agarose Products EZ PAGE Gel Cassettes EZ Agarose Gel Cassettes EZ PAGE Electrode Cap EZ Agarose Electrode Cap EZ PAGE Sample Loading Buffer 4x LB1 EZ Agarose Sample Loading Buffer 5x LB2 Precise Protein M W Marker unstained MKPO01 100 bp DNA M W Marker MKDOO5 Low Range Protein M W Marker unstained MKP002 250 bp DNA MMW Marker MKD004 Pre stained Protein M W Marker MKP003 1 Kb Plus DNA M W Marker MKDOO1 Warranty amp Liability This product was produced utilizing the highest practical standards of materials workmanship and design EZBiolab Inc warrants that the product has been tested and will meet or exceed published specifications This warranty is valid only if the product has been operated and maintained according to the instructions provided EZBiolab warrants EZ PAGE and EZ Agarose Electrode Caps for one year from the date of shipment and the disposable products to be free from defects in materials and workmanship under recommended storage and use conditions until the product expiration date If the product proves defective during this period EZBiolab Inc will repair or replace it at our option free of charge This warranty does not cover damage in transit damage caused by carelessness misuse or neglect normal wear through frequent use damag
7. lastic block i e the buffer dam The buffer dam must be opened to allow the buffer to connect with the gel matrix for electrophoresis The dam has been scored for easy opening A special tool the buffer dam opener is included in each package to facilitate this process Insert the buffer dam opener so that the dam is placed between the two blades of the buffer dam opener Firmly but slowly rotate the buffer dam opener towards the far side of the gel to snap the buffer dam Once the dam is opened stop the rotation EZ Agarose User Manual Note The bottom of the buffer dam may still be attached and hang in the buffer reservoir This will not interfere with electrophoresis A sample loading guide has been built in the gel cassette to facilitate sample loading in the wells The maximum loading volume is 20 uL 1 Place the EZ Agarose Electrode Cap which contains the electrode wires on the top of the cassette This will result in the electrode wires being inserted into the buffer Be careful not to spill the buffer Make sure the cap is firmly positioned on the top of the cassette EZ Agarose User Manual Note If the buffer dam is still attached it will be pushed to the bottom of the tray when the electrode cap is seated on the EZ Agarose Gel Cassette This will not affect the run 2 Insert the female end of each color coded cable onto one of the two electrode rods on the EZ Agarose Electrode Cap and the male end of
8. ose 20 bp 1 kb Dye Ethidium bromide Buffer TBE Maximum loading volume 20 uL Shelf Life and Storage Model L 18 months Model R 8 months Stored at 4 C 8 C Do not freeze Product use For research use only not intended for diagnostic use EZ Agarose User Manual Getting Started You Will Need 7 EZ Agarose cassette at room temperature 7 EZ Agarose Electrode Cap purchased separately Cat No E207 includes pre installed electrodes and two color coded cables m Electrophoresis power supply any power supply can be used 7 Sample loading buffer any sample loading buffer can be used use EZ Agarose Sample Loading Buffer for best results Cat No LB2 m DNA molecular weight markers if using DNA molecular weight markers are available from EZBiolab Cat No MKD001 Preparing Your Samples For best results use sample loading buffer from EZBiolab Other common sample loading buffers such TBE can also be used Prepare samples according to standard protocols for agarose gel electrophoresis Preparing for Electrophoresis Opening the EZ Agarose Cassette 1 Remove the EZ Agarose Cassette from the package and place it on a lab bench EZ Agarose User Manual 2 Remove the top seal slowly to avoid spilling the running buffer 4 Model L only for Model R skip this section and go directly to the next section The gel matrix is separated from the anode buffer reservoir bottom of the gel by a p
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