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External devices tutorial

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1. button to interrupt this command The Prepare command will ignore all the ports of a Dosino for which the Active status has been disabled in the Liquid Handling setup see Section 2 4 1 2 5 2 2 Dosino dose This command delivers the specified volume in ml through the specified port If the volume exceeds the dosing unit volume the Dosino will be refilled using the Fill port before the dosing command is resumed If a negative value is defined the volume will be aspirated Figure 17 shows how to use the Dosino dose command Dosino dose Analyte 5 1 Device name Analyte r volume mili z Fort 1 gt Figure 17 Using the Dosino dose command 18 Page NOVA External devices tutorial While the Dosino is dispensing the requested volume through the specified port the progress will be displayed in the Autolab displayed see Figure 18 Autolab display ie A Analyte Prepare Fill Empty Dose iml 5 Dose Fart 1 Output port Dosed ml 1 67 Hald stop 9128 BUSY 20 ml Figure 18 The dosing progress is indicated in the Autolab display Pressing the button will interrupt the dosing command The dosing process will hold until the e button is clicked see Figure 19 Autolab display a A Analyte Prepare Fill Empty Dose iml F Dose Fart 1 Output port Dosed ml Ue 9128 snui 20 ml Figure 19 The dosing process is resumed when the _ _ button is clicked Pressing the button wi
2. Dosino prepare This command prepares the dosing unit by rinsing and filling the connected tubing and the dosing cylinder The tubing system of the Dosino should be freed from air bubbles at least once per day by carrying out a preparation cycle This is a process that could take some time It is recommended to use this command at the beginning of the procedure During the preparation process the dosing cylinder as well as the connected tubings are completely filled Several filling and dosing processes can be carried out The volumes required for this are calculated from the settings specified in the Liquid Handling Setup refer to Section 2 4 1 Figure 15 shows an overview of the Dosino prepare command used in a procedure 17 Page NOVA External devices tutorial Dosino prepare Analyte Device name Analyte Cycles 3 lt gt Figure 15 Using the Dosino prepare command The number of Prepare cycles can be specified When the command is executed during a procedure the progress of the Prepare command can be tracked in the Autolab display panel see Figure 16 Autolab display a A Analyte Frepare Fill Empty Dose imh A Dose Fort 1 Output port Dosed ml 0 00 Hald stop 9128 BUSY 20 ml Figure 16 The Autolab display indicates the progress of the Prepare command It is possible to click the button to hold the Prepare command or to press the s
3. Using the Sample processor valve command Four values can be specified for the Valve parameter e 00 valve 1 off valve 2 off e 01 valve 1 off valve 2 on 10 valve 1 on valve 2 off 11 valve 1 on valve 2 on 2 6 2 4 Sample processor 858 inject valve This command controls the optional injection valve This command can only be used in combination with the Metrohm 858 Professional Sample Processor When installed this valve is located on the side of the sample processor tower see Figure 36 29 Page NOVA External devices tutorial Figure 36 Location of the Injection valve Metrohm 858 Professional Sample Processor only The injection valve can be set in two different mode Fill and Inject see Figure 36 Figure 37 shows an overview of the Sample processor 858 inject valve command used in a procedure El Sample processor 056 injectvalve Sample processor Device name Sample processor E _ Valve ol E Figure 37 Using the Sample processor 858 inject valve command Setting the Valve parameter to 0 switches the valve to the Fill position while setting the Valve parameter to 1 switches the valve to the Inject position see Figure 36 30 Page NOVA External devices tutorial 2 6 2 5 Sample processor pump This command controls the pumps of the specified tower The pumps can be built in or external see Figure 38 Pump 1 Pump 1 Pump 2
4. EO OE el C4 lt Figure 101 The backplane of the pAutolab type III The location of the BNC plugs is slightly different in the case of the uAutolab type Il The analog input Vin and analog output Vout are identified by the same labels as those shown in Figure 100 77 Page NOVA External devices tutorial 4 5 PGSTAT101 204 and M101 M204 The PGSTAT101 PGSTAT204 and the M101 M204 potentiostat galvanostat module for the Multi Autolab are fitted with a front panel connection that can accommodate an optional input output cable see Figure 102 Figure 102 The optional I O cable for the PGSTAT101 top and PGSTAT204 M101 M204 bottom This optional cable provides two BNC connections for an external signal input Vin and an external signal output Vout respectively 4 6 Hardware setup for external devices To control an external device using the Autolab DAC164 Vout or to read an analog signal from an external device using the Autolab ADC164 Vin requires an adjustment of the Hardware setup Depending on the type of instrument used the External additional module can be selected and adjusted in the Hardware setup see Figure 103 78 Page File Tools Main Module Y PGSTAT302N PGSTAT302F PGSTAT302 PGSTAT30 _ PGSTAT30 AUT9 _ PGSTAT128N PGSTAT12 _ PGSTAT100N PGSTAT100 _ PGSTAT100 AUT9 _ PGSTAT101 M101 _ wAutolab III _ wAutolab
5. 10 V Furthermore the Autolab is also fitted with an Analog to Digital converter ADC which can be used to read an analog voltage from an external device in the range of 10 V The DAC output s and ADC input s can be used to communicate to external devices fitted with matching inputs and outputs Depending on the model the following DAC outputs or ADC inputs are available see Table 2 of DAC of ADC Instrument outputs inputs PGSTAT10 20 30 100 AUT9 series 2 DAC164 2 ADC164 PGSTAT12 128N 30 302 302N 100 100N 2 DAC164 2 ADC164 PGSTAT302F 2 DAC614 2 ADC164 uAutolab II I 1 Vout 1 Vin PGSTAT101 M101 special cable required 1 Vout 1 Vin PGSTAT204 M204 special cable required 1 Vout 1 Vin Table 2 Overview of the analog outputs and inputs located on the different Autolab instruments 4 1 Autolab PGSTAT10 PGSTAT20 PGSTAT30 PGSTAT100 The first generation Autolab PGSTAT instruments are identified by a serial number starting by AUTY followed by five digits and are fitted inside a 19 inch rack cabinet illustrated in Figure 98 75 Page NOVA External devices tutorial ADC164 DAC164 Figure 98 Front panel overview of the series AUT9 instruments These instruments are fitted with a DAC164 and an ADC164 Both modules provide a total of four BNC connectors on the front panel labeled 1 through 4 The first two BNC connectors of the DAC164 and the ADC164 are reserved but the two
6. not shown in Figure 27 2 6 2 Procedure Sample processor control Dedicated commands are available in the Metrohm devices group of the command browser see Figure 13 24 Page NOVA External devices tutorial sample processor litt Sample processor mowe fe sample processor valve sample processor 056 inject valve e sample processor pump sample processor 050 peristaltic pump Sample processor swing Sample processor stir Figure 28 The Sample processor related commands are located in the Metrohm devices group The following Sample processor control commands are available e Sample processor lift defines the position of the lift of the Sample processor e Sample processor move rotates the sample rack to the defined position e Sample processor valve controls the valves of the Sample processor e Sample processor 858 inject valve toggles the optional injection valve of the Metrohm 858 Professional Sample Processor between the n ect position and the 7 position e Sample processor pump controls the pumps of the Sample processor or the pumps connected to the Sample processor e Sample processor 858 peristaltic pump controls the optional peristaltic pump of the Metrohm 858 Professional Sample Processor e Sample processor swing controls the position of the Swing head e Sample processor stir controls the stirrer of the Sample processor e Sample processor close termi
7. see Figure 86 64 Page NOVA External devices tutorial amp Edit Options 0 Automatic Current Ranging Autolab control Automatic Integration Time WE 1 Highest current range TmA Lowest current range 100 nA Highest current range select the highest current range for automatic current ranging Cancel Figure 86 The Edit options window Click the Autolab control tab in the editor to display the controls on this tab see Figure 87 amp Edit Options 0 Figure 87 The Autolab control tab 65 Page NOVA External devices tutorial On the Autolab control tab the following items can be added to a list of actions to perform at each data point e Event counter a counter that tracks the number of data points and which triggers and action when the counter reaches a user defined condition e Autolab control a generic Autolab control adjustment executed at each data point e Pulse a control for sending a TTL pulse of a user defined duration on a DIO connector of the Autolab at each data point It is possible to combine several items on the Autolab control tab To define the TTL pulses to be used to synchronize the acquisition of spectra by the Avantes spectrometer during the measurement command select the Event counter from the drop down list and click the Add button to add it to the Autolab control list of items see Figure 88 gt Edit Options 0 Automatic Current Ranging Cutoff A
8. settings E Input box Tithe of box Message Value Time limit 5 Use time limit B External device send Device name Command NOVA External devices tutorial Julabo RS232 Julabo RS2 JZ r entries Julabo temperature Temperature of the bath 30 Mo a Julabo out_sp_O00 104 Julabo out_sp_00 0 eu Figure 142 Adding a linkable parameter to the data string To create a linkable parameter in the data string replace the value in the string by x where x is an integer between O and 4 An additional parameter x will be added to the command This new parameter is linkable The example shown in Figure 142 illustrates the use of this feature The 0 parameter is linked to the value defined in the Input box 6 3 External device receive This command can be used to receive a data string from the external device To receive a string from the external device the External device send is first used to send a specific string to the external device The External device receive command is then added to the procedure to read the reply string from the external device see Figure 143 E External device initialize Julabo R5232 Device name Julabo Device type FECE cg settings r entries m E External device send Julabo in_sp_O0 Device name Julabo Command In_sp_O0 External device receive Julabo 10 Device name Julabo Command T0 Uy E Message box Title of box Temperature Me
9. state represented by a 0 or a 1 respectively The state of the 14 output lines is specified as a 14 character string consisting of O and 1 representing the state of the output lines from OUT13 to OUTO see Figure 58 Remote outputs Device name Remote box Outputs lt gt Figure 58 Using the Remote outputs command The specified state of the 14 output lines is persistent until changed or until the Remote Box is powered down or reset 2 9 Parallel operation For each Metrohm device connected to the computer it is possible to define if Parallel operation is enabled or disabled This setting must be defined for each individual device in the Liquid Handling setup window see Figure 59 43 Page NOVA External devices tutorial Analyte 800 9126 Remote box 770 45892 Stirrer 801 2358 Refresh Liquid Handling setup Name Port 1 settings Rate 0 01 ml min 166 ml min Tube length mm Tube diameter mm Port settings Rate 0 01 ml min 166 ml min Tube length mm Tube diameter mm Port 3 settings Rate 0 01 ml min 166 ml min Tube length mm Tube diameter mm Pornt4 settings Rate 0 01 ml min 166 ml min Analyte 2 0 200 0 2 0 330 0 2 0 Y rallel execution Active Dosing port we Active Fill port vd Active special port ad Active None M OK Cancel Figure 59 The Liquid Handling setup can be used to enable or disabl
10. 75 5 w Seral number a Start wavelength nm 250 Stop wavelength nm 600 Integration time ms 500 Number of averages 1 ae v Avantes Spectrometer setup Basic settings Advanced settings Serial number start wavelength 250 nm stop wavelength 600 nm Integration time 500 ms Cancel Figure 75 The Avantes initialize command To initialize the Avantes spectrometer a number of parameters must be specified This is done using a dedicated editor Click the button located next to the Avantes initialize command in the procedure editor see Figure 75 The Avantes Spectrometer setup window will be displayed see Figure 76 55 Page NOVA External devices tutorial Vv Avantes Spectrometer setup Basic settings Advanced settings Serial number start wavelength nm stop wavelength 600 nm Integration time 500 ms OR X Cancel Figure 76 The Avantes Spectrometer setup window The following basic settings must be defined on the Basic settings tab of the Avantes Spectrometer setup window see Figure 77 e Serial number the serial number of the Avantes spectrometer used in the measurement The serial number is indicated on the label located on the bottom of the spectrometer e Start wavelength nm the start wavelength of the measurement segment in nm This value must be smaller than the Stop wavelength and should fit within the measurable range of the spectrometer e Stop wavelength nm the stop wave
11. External Figure 38 Location of the pumps left one built in and one external right two built in Figure 39 shows an overview of the Sample processor pump command used in a procedure El sample processor pump Sample processor Device name sample processor Tower 1 o E Figure 39 Using the Sample processor pump command Four values can be specified for the Pump parameter e 00 pump 1 off pump 2 off e 01 pump 1 off pump 2 on e 10 pump 1 on pump 2 off e 11 pump 1 on pump 2 on gt The pumps can be used for rinsing the titration head and aspirating off samples that have been processed The necessary tubing is supplied with the corresponding Metrohm instruments refer to the Metrohm Installation Instructions for more information 31 Page NOVA External devices tutorial The following external pumps can be connected to the back plane of the sample processor tower when no built in pumps are present e 823 Membrane pump e 772 Peristaltic pump e 843 Pump station Membrane or Peristaltic 2 6 2 6 Sample processor 858 peristaltic pump This command controls the optional peristaltic pump This command can only be used in combination with the Metrohm 858 Professional Sample Processor When installed this pump is located on the side of the sample processor tower see Figure 40 i Ssiona l Sample Processor swissmade Figure 40 Location of the Pe
12. II _ PGSTAT204 _ M204 PGSTAT20 PGSTAT10 _ PSTAT10 Import FRA2 Calibration C ProgramData Metrohm Autolab 11 0 HardwareSetup xml Additional Module s E _ FRA32M FRA2 ADC10M ADC750 ADC750r4 SCAN250 SCANGEN BA BIPOT ARRAY ECD FI20 Filter W F120 Integrator Booster20A Booster10A Power Supply Frequency 50 Hz Hardware setup Oo EA ADC164 1 Signal name v A Signal unit V Conversion slope 1 VN Conversion offset 0 V ADC164 2 Signal name v Signal unit V Conversion slope 1 VN Conversion offset 0 V DAC164 1 Signal name v A Signal unit V Conversion slope 1 VN Conversion offset 0 V Upper limit 10 V Lower limit 10 OK Auto Cancel 5V NOVA External devices tutorial Figure 103 Defining the parameters for the External module in the Hardware setup Predefined settings are available for the Autolab RDE and the Autolab LED Driver The settings for the ADC164 Vin inputs and the DAC164 Vout outputs can be defined independently 4 6 1 ADC164 Vin settings The settings for the ADC164 input or the Vin input depending on the type of instrument can be defined in the dedicated panel on the right hand side of the Hardware setup window see Figure 104 ADC164 1 signal name w bi Signal unit V Conversion slope 1 VN Conversion offset 0 V Figure 104 The settings for the ADC164 input 79 Page NOVA E
13. Setup 2 7 2 1 Stirrer speed This command defines the rotation rate and direction of the stirrer The speed is defined by entering an integer between 15 and 15 The actual rotation rate is the entered value multiplied by a speed change per step coefficient which is the case of the 801 Stirrer is 180 RPM step 10 Factory default value 39 Page NOVA External devices tutorial If the speed value is positive the stirrer will rotate in the clockwise direction A negative value will rotate the stirrer anti clockwise Figure 52 shows an overview of the Stirrer soeed command used in a procedure E Stirrer speed Stirrer 0 Device name stirrer Figure 52 Using the Stirrer speed command 2 8 Metrohm 6 2148 010 Remote Box The current version of NOVA supports the Metrohm 6 2148 010 through the MSB interface see Figure 47 Figure 53 Metrohm 6 2148 010 Remote box The Remote Box can be controlled in a single way e During a NOVA procedure using the provided commands 2 8 1 Procedure Remote Box control Two dedicated commands are available in the Metrohm devices group of the command browser see Figure 54 i Metrohm devices Remote inputs Remote outputs Figure 54 The Stirrer related command is located in the Metrohm devices group 11 Metrohm devices with MSB connectors cannot be connected to the computer directly These devices must be connected to a Metrohm instrument with USB interf
14. Tube diameter mm 50 Fort 3 settings Rate 0 01 ml min 166 ml min 20 0 Active Tube length mm 330 0 Special port a Tube diameter mm 2 0 Port4 settings Rate 0 01 ml min 166 ml min 20 0 Active None w Figure 4 Overview of the settings and parameters for a Metrohm 800 Dosino The following settings can be specified see Figure 4 e Name of the device a unique name to identify the instrument in NOVA By default the name of the device corresponds to the serial number of the device until a user defined name is assigned to the device e Parallel execution specifies if the parallel execution is allowed for this device refer to Section 2 9 for more information e Role of each port the role of each port specified using the provided drop down list The following port roles are available Dosing port Fill port Special port or none e Rate Tube length and Tube diameter the settings of the tubings connected to the ports of the Dosino see Figure 5 e Active each port can be activated or deactivated using the provided checkbox The Active checkbox provided in the Dosino setup Is only relevant for the Prepare and Empty actions of the Dosino When a port is set to inactive the port will be Skipped during these stages 9 Page NOVA External devices tutorial Make sure that the roles are defined properly since this will have an influence on the Prepare Fill and Empty commands Figure 5 shows the location of the
15. and Avantes 3648 Spectrometers through the USB interface Dedicated commands are available in the External devices group of the command browser see Figure 74 s External devices Avantes initialize m Avantes clase External device initialize External device send External device receive External device close Figure 74 The Avantes related commands are located in the External devices group The following Avantes control commands are available e Avantes initialize used to setup the Avantes spectrometer and initialize the control e Avantes close terminates the Avantes control 13 Refer to the Avantes user manual for more information 54 Page NOVA External devices tutorial All the Avantes control commands are tagged as ntermediate commands This tutorial does not cover the use of the Avantes spectrometers The reader is invited to refer to the AvaSpec user manual supplied with the instrument It is highly recommended to use the AvaSoft to determine the most suitable settings to be used in combination with the Autolab instrument optical fiber diameter light source etc Any information regarding the experimental conditions required to operate the Avantes spectrometers falls outside of the scope of this tutorial 3 3 1 Avantes initialize To control an Avantes spectrometer with NOVA the instrument unit must be initialized This is done using the Avantes initialize command see Figure
16. and pin 11 Port B includes 4 read lines and an isolated ground pin pin 3 All pins on Port B are galvanically isolated Pin 4 or pin 11 should not be used as ground pin for the read lines 90 Page NOVA External devices tutorial f Warning The write lines of the PGSTAT101 PGSTAT204 M101 and M204 DIO connector are capable of supplying a maximum current of 200 mA Suitable pull down resistors should be placed in the write lines of the DIO cable connected to the PGSTAT101 PGSTAT204 M101 and M204 A typical value for the pull down resistance is about 1 kQ Please refer to the user manual of the external device connected to the instrument for more information 5 3 DIO trigerring Each pin on the DIO connector s of the Autolab can be set to two different levels e Low 0 V this is the default status of each pins when the Autolab is initialized This status corresponds to a digital 0 e High 5 V this status corresponds to a digital 1 The maximum current load on each pin is 2 5 mA for all the PGSTAT instruments except for the PGSTAT101 PGSTAT204 M101 and M204 for which the maximum load current is 200 mA Depending on the communication protocols an external device connected to the Autolab can be triggered by a down to up transition or an up to down transition 5 3 1 Port initialization Before a DIO connector can be used to send or receive TTL triggers the ports involved must be initialized Two
17. available in the Sampler as External 1 signals as shown in Figure 114 84 Page NOVA External devices tutorial i Edit Sampler 0 Signal Sample Optmized d dt Sampler configuration WE 1 Current Sampler WE 1 Potential Segment WE 1 Power _ External 1 Autolab LED Driver WE 1 Resistance C Segment Optimized WE 1 Charge O WE 1 Current External 1 Autolab LED Driver seo Time External 1 Autolab Default Time _ Sample alternating Figure 114 Adding an external signal to the sampler The external signal is measured in the units defined in the Hardware setup Since the signal is measured like any other electrochemical signal it can be used in data plots and cutoff values can be applied on this external signal 4 8 Sending external signals Once the settings have been defined in the Hardware setup as explained in Section 4 6 2 a control signal can be sent to an external device through the available output DAC DAC164 or Vout The external signal can be specified in the units defined in the Hardware setup The signal can be sent in two different ways 1 Using the provided manual control 2 Using a dedicated command in a NOVA procedure 4 8 1 Manual control of the DAC164 Vout Manual control of the external device can be achieved using a dedicated extension of the Autolab display To activate or deactivate the additional panel select the corresponding option from the View menu see F
18. different modes are available e Read R the port is initialized to read mode and will be used to receive TTL triggers e Write W the port is initialized to talk mode and will be used to send TTL triggers Initialization of a port is performed in the Autolab control window in the DIO section see Figure 124 91 Page NOVA External devices tutorial have Autolab control 0 PGSTATSO2ZN AJ DIO P1 BHS s Section A w 00000000 125 Summary i Section B R 00000000 2 4A Section C NA 00000000 i23 w DIO P2 Figure 124 Initialization of the port is done in the Autolab control window Each individual port can be set to read R or write W independently It is recommended to initialize the DIO ports at the beginning of the procedure By default the ports are set to write W This setting is not available for the PGSTAT101 PGSTAT204 M101 and M204 since the pins of the DIO connector are pre configured to input and output see Section 5 2 Ports A and B are initialized at startup 5 3 2 Sending triggers To send TTL triggers the Auto ab contro command must be used Before a DIO port can be used to send a TTL trigger it must be initialized into write direction see previous section The Autolab control window is used to define the DIO port status according to an 8 bit binary string or the converted decimal value corresponding to this string The string is specified directly in the Auto
19. instrument see Figure 27 23 Page NOVA External devices tutorial Autolab display x A sample processor Tower 1 Rack position 1 43 0 Mowe Lift position 0 125 0 0 Move Wr S H Pump Peristaltic pump 9 gt Hold stop 4589 000000 Figure 27 Manual control of a Sample processor The Sample Processor manual control panel provides the following controls Move the rack to the specified position with respect to the Sample processor tower using the button Move the lift to the specified position using the button Three shortcut buttons are available o moves the lift to the Work position defined in the Liquid Handling Setup for the device using the current rack position o moves the lift to the Shift position default O0 mm using the current rack position o 4 moves the lift to the Shift position default 0 mm and moves the rack to position 1 This is defined as the Home position Switch on or off Pump 1 and or Pump 2 if present using the dedicated checkboxes shown in Figure 27 Switch on or off Valve 1 and or Valve 2 if present using the dedicated checkboxes not shown in Figure 27 Set the Stirrer speed between 15 and 15 if present not shown in Figure 27 Set the Peristaltic pump speed between 15 and 15 if present only for the Metrohm 858 Professional Sample Processor Set the Injection valve to Fill or Inject position if present only for the Metrohm 858 Professional Sample Processor
20. lower BNC connectors are available for connections to external devices 4 2 Autolab PGSTAT12 PGSTAT30 302 PGSTAT100 The second generation Autolab PGSTAT instruments are identified by a serial number starting by AUT7 followed by four digits and are fitted inside a cabinet illustrated in Figure 99 00900009000 ADC164 DAC164 Figure 99 Front panel overview of the series AUT7 instruments These instruments are fitted with a DAC164 and an ADC164 Both modules provide a total of two BNC connectors on the front panel labeled 1 and 2 76 Page NOVA External devices tutorial 4 3 Autolab PGSTAT128N PGSTAT302N PGSTAT302F PGSTAT100N The third generation Autolab PGSTAT instruments are identified by a serial number starting by AUT8 followed by four digits and are fitted inside a cabinet illustrated in Figure 100 COGOVOVGOU O ADC164 DAC164 Figure 100 Front panel overview of the series AUT8 instruments These instruments are fitted with a DAC164 and an ADC164 Both modules provide a total of two BNC connectors on the front panel labeled 1 and 2 4 4 yAutolabll and Ill The pAutolab type Il and type Ill instruments are identified by a serial number starting by u2AUT7 and u3AUT7 respectively followed by four digits and are fitted inside a compact cabinet The external analog input Vin and output Vout are located on the backplane of the instrument as shown in Figure 101 Vout Eout lout
21. rotation rate and direction of the optional 802 propeller stirrer on the specified tower see Figure 44 CE Figure 44 The Metrohm 802 propeller stirrer The 802 stirrer connects to the dedicated connector on the back plane of the sample processor tower see Figure 45 34 Page NOVA External devices tutorial Stirrer connection Figure 45 The 802 propeller stirrer connects to the backplane of the sample processor tower The speed is defined by entering an integer between 15 and 15 The actual rotation rate is the entered value multiplied by a speed change per step coefficient which is the case of the 802 Stirrer is 140 RPM step If the speed value is positive the stirrer will rotate in the clockwise direction A negative value will rotate the stirrer anti clockwise Figure 46 shows an overview of the Stirrer soeed command used in a procedure cl Sample processor stir Sample processor Device name sample processor ma Tower 1 ol Sa Figure 46 Using the Sample processor stir command 2 7 Stirrer control The current version of NOVA supports the Metrohm 801 Magnetic stirrer through the MSB interface see Figure 47 Factory default value 8 Metrohm devices with MSB connectors cannot be connected to the computer directly These devices must be connected to a Metrohm instrument with USB interface or to a dosing interface with USB Refer to Section 2 for more information 35 Page NOVA Exter
22. the slider 37 Page NOVA External devices tutorial 2 7 2 Procedure Stirrer control A dedicated command is available in the Metrohm devices group of the command browser see Figure 50 me etrohm devices Stirer speed Figure 50 The Stirrer related command is located in the Metrohm devices group The following Stirrer control command is available e Stirrer speed set the rotation speed of the stirrer The Stirrer soeed command is an ntermediate commands To use this command the device name needs to be specified The identifying name can be entered directly in the procedure editor or can be selected by clicking the button to display a list of available devices see Figure 51 Refer to the Metrohm Stirrer user manual for more information 38 Page NOVA External devices tutorial Commands Parameters Links New procedure Remarks End status Autolab signal sampler Time WE 1 Current Options No Options Instrument Instrument description Stirerspeed Stirrer_1 0 Stirrer_ Speed 0 lt gt Device name x Please select one item Sample Processor Analyte Remote box Stirrer Cancel Figure 51 A list of available devices is displayed in the procedure editor Select the name of the device to control and click the __ button to close the editor The name displayed in the Device name dialog correspond to the names for each device specified in the Liquid Handling
23. the Avantes device driver 1 3 Follow the instructions indicated on screen When prompted install the driver by clicking the install button see Figure 65 Windows Security Would you like to install this device software Name Avantes Publisher Avantes Always trust software from Avantes Install N Don t Install You should only install driver software from publishers you trust How can I decide which device software Is safe to install Figure 65 Installation of the Avantes device driver 2 3 At the end of the installation process the driver should be correctly installed This is indicated in the final dialog see Figure 66 49 Page NOVA External devices tutorial Device Driver Installaton Wizard Completing the Device Driver Installation Wizard The drivers were successtully installed on this computer ou Can now connect your device to this computer your device came with instructions please read them first Driver Name status y Avantes Win JSB AvantesSpectrometers Ready to use WT Figure 66 Installation of the Avantes device driver 3 3 Connect the Avantes spectrometer to the computer using the provided USB cable When the instrument is connected for the first time the driver will be installed Once the driver is ready for use NOVA can control it The green Power LED located on the front panel of the AvaSpec must be lit see Figure 67 Power Scan Figu
24. to update the firmware Please check the Windows Device Manager to identify the port number Start the FW up5276 exe application A window will be displayed see Figure 94 5 x Open Port Close Update Firmware Figure 94 The firmware updater application Specify the COM port number in the field provided in the top left corner of the application COM1 is used by default Click the Open Port button to open the communication to the spectrometer A message will be displayed see Figure 95 72 Page NOVA External devices tutorial v AS5216 Firmware Updater el File Help Port com Open Port Close AS5216 Firmware Upd Update Firmware Please toggle the Power Supply c q USB cable to your AS52116 Figure 95 Click the Open Port button to open the communication with the spectrometer As indicated by the application remove the USB cable from the computer to power down the spectrometer Wait a few seconds then plug the USB cable in the computer again to power up the spectrometer again Click the OK button to continue see Figure 95 Now click the Update Firmware button to update the firmware The firmware will be uploaded to the spectrometer A progress bar will be displayed see Figure 96 73 Page NOVA External devices tutorial o x Update Firmware a i a i p m ii l D uw i Figure 96 The firmware
25. 2 A X cut ps a Pas Lil J F New item E Open HH select all Copy path a 7 Easy access A i Select none Copy Paste Move Copy Delete Rename New Properties o Paste shortcut to tor z TE Lg History go Invert selection J gt ThisPC gt OS C gt Avantes Firmware for Autolab v Search Avantes Firmware for 2 x Favorites Name Date modified Type Size AS5216SW bin 6 12 2013 11 54 BIN File 158 KB a8 Homegroup m Avantes AS5216 Firmware update utility doc 30 10 2007 16 56 Microsoft Word 97 48 KB Ma FWup5216 exe N 30 10 2007 16 26 Application 531 KB is This PC amp FWup5216 ini 14 10 2014 09 04 Configuration setti 1 KB PROTOCOL DLL 11 4 2000 19 01 Application extens 64 KB Network 3 SUPERCOM DLL 9 3 2000 11 37 Application extens 72 KB 6items 1 item selected 531 KB Figure 93 Download and copy all the files in the same folder on the computer To update the firmware a COM port RS232 must be present on the computer A USB to RS232 converter can be used 71 Page NOVA External devices tutorial Connect the Avantes spectrometer to the computer using the provided USB cable The Power indicator on the spectrometer will be lit indicating that the spectrometer is powered Connect the interface cable to the DB26 connector located on the back plane of the Avantes spectrometer Connect the DB9 end of this interface cable to the COM port on the computer The COM port number must be known in order
26. 4589 Analyte 800 9128 Remote box 770 45892 X stirrer 801 2358 Figure 11 Manual control of the Liquid Handling devices is available from the View menu Connected devices are identified as Name Metrohm Device type Serial Number The Autolab display will be displayed with an additional panel for the selected instrument see Figure 12 Autolab display x A Analyte Prepare Fill Empty Dose mill z Fort 2 Input port Diosed ml 5 00 Hola stop 9128 40 ml Figure 12 Manual control of a Dosino The Dosino manual control panel provides the following controls 15 Page NOVA External devices tutorial e Prepare the Dosino using the button e Fill the Dosino through the specified filling port using the button e Empty the Dosino using the button e Specify and dose a volume using the button through the specified port using the provided drop down box e The and buttons can be used to hold or stop the currently running actions on the Dosino 2 5 2 Procedure Dosino control Dedicated commands are available in the Metrohm devices group of the command browser see Figure 13 gt Dosino empty Diosino fill 2 Dosino to end fan Diosino exchange Figure 13 The Dosino related commands are located in the Metrohm devices group The following Dosino control commands are available e Dosino prepare fills the dosing cylinder and the tubings e
27. 50 Figure 7 A Metrohm Sample rack 6 2041 350 e Lift rate shift rate and swing rate the lift rate shift rate and swing rate settings for each tower e Work position and position limit specifies the work position and the position limit for each tower e Additional settings the presence of pumps valves stirrer peristaltic pump 858 only and injection valve 858 only using the provided checkboxes e Active towers can be deactivated if needed using the provided checkbox When a Metrohm 858 Professional Sample Processor is used Tower 2 is deactivated by default 2 4 3 Stirrer Hardware setup The Liquid Handling setup window can be used to specify the settings for the Metrohm 801 Stirrer see Figure 8 Name Figure 8 Overview of the settings and parameters for a Metrohm 801 Stirrer The following settings can be specified see Figure 8 12 Page NOVA External devices tutorial e Name of the device a unique name to identify the instrument in NOVA By default the name of the device corresponds to the serial number of the device until a user defined name is assigned to the device 2 4 4 Remote box setup The Liquid Handling setup window can be used to specify the settings for the Metrohm 6 2148 010 Remote Box see Figure 9 Name Figure 9 Overview of the settings and parameters for a Metrohm 6 2148 010 Remote Box The following settings can be specified see Figure 9 e Name of t
28. 60 6 1462 170 Swing Angle offset 8 8 0 8 8 en aa 117 117 84 73 73 Swing radius 112 mm 112 mm 110 mm 127 mm 127 mm Swing direction gt 2 gt Table 4 Overview of the advanced Robotic arm settings Depending on the type of Sample processor Sample rack and Robotic arm it may be necessary to manually edit the configuration files of the Sample processor These files are automatically generated when the Sample processor is configured for the first time in the Liquid Handling setup see Section 2 4 2 The files are located in the following hidden folder C ProgramData Metrohm Autolab 12 0 Two files are generated These files are automatically called xxxxConfigInput xml and xxxxConfigOutput xml where xxxx corresponds to the last four or five digits of the Sample processor serial number In order to properly configure the Sample processor it may be necessary to manually edit these files To edit each file used to control the Sample processor the following steps must be followed 1 Make sure that both files are located in the folder If not please refer to Section 2 4 2 for more information 2 Use a text editor for example Notepad to edit the xm1 files see Figure 147 Ej 4589ConfiglInput xml Notepad oO EA File Edit Format View Help lt xml version 1 0 encoding UTF 8 gt lt MetrohmSamplerConfig xmlns xsi http www w3 0rg 2001 XMLSchema instance xsi noNamespaceSchemaLocation M
29. 770 45892 Name 9128 _ Parallel execution 2358 801 2358 Port 1 settings Rate 0 01 ml min 166 ml min 20 0 Active Tube length mm 330 0 Dosing port v Tube diameter mm 2 0 Port 2 settings Rate 0 07 ml min 166 ml min 150 0 Active Tube length mm 200 0 Fill port w Tube diameter mm 20 Fort3 settings Rate 0 07 ml min 166 ml min 20 0 Active Tube length mm 330 0 Special port y Tube diameter mm 2 0 Port4 settings Rate 0 07 ml min 166 ml min 20 0 Active None v Refresh Cancel Figure 3 The Liquid Handling setup The connected devices are identified by the following string Device name Metrohm Device type Device serial number The Device name is the same as the Device serial number until a user defined name is assigned to the device This window can be used to specify the settings of each connected device and store these settings locally Once the settings are defined for a Metrohm device they will be used for this device each time it is used in NOVA 2 4 1 Dosino Hardware setup The Liquid Handling setup window can be used to specify the settings for the Metrohm 800 Dosino see Figure 4 8 Page NOVA External devices tutorial Name 9128 _ Parallel execution Port 1 settings Rate 0 01 ml min 166 ml min 20 0 Active Tube length mm 330 0 Dosing port r Tube diameter mm 2 0 Port settings Rate 0 01 ml min 166 ml min 150 0 Active Tube length mm 200 0 Fill port W
30. Avantes close command provides three signals recorded by the spectrometer e Spectrum number the number of each spectrum starting from 1 e Wavelength the wavelengths used in the experiment for each spectrum The measured range is defined in the Avantes initialize command The actual range is defined by the closest wavelengths available with the connected Avantes spectrometer and these can change from one spectrometer to another e Measured value the measured intensity at each wavelength in arbitrary units These values correspond to the measured counts for each wavelength The value can be between 0 and 16384 14 Bit ADC The values recorded in NOVA can differ by a factor of 4 from the values recorded using similar acquisition parameters in the AvaSoft software where values are converted on 16 Bits If the signal leading the experiment is linked to the Input reference signal parameter of the Avantes close command the electrochemical data can be correlated to the 61 Page NOVA External devices tutorial spectroscopic data In order for this to work properly the Input reference signal step parameter must be adjusted properly In the example shown in Figure 82 the Input reference signal should be linked to the Potential applied signal and the Input reference signal step should be set to 9 Figure 83 shows an example of a procedure involving an Avantes spectrometer il Avantes initialize z Serial number 1105110U1 Start wave
31. CONTO eases E EE o 22 2 6 1 Sample processor manual control 00 0 cece ce ceeeeee ee eeeeeeeeeeeeaneeeeeaes 23 2 6 2 Procedure Sample processor CONTIOL ccccceceecceeeeeeeeeeeeeeeeeeeeeeeaes 24 2 6 2 1 Sample processor MIN sete cere naeiioeenctitne asorcuseseseesesstseeeusieveten one 26 2 6 2 2 Sample processor MOVE cecccceeeeeceeeeeeeeseeeeeeseeeeeesneeeeeaneeeeeaas 27 2 6 2 3 Sample processor valve ccccccsececceeseeccesseeseeseeeseeneeeseeneeesees 28 2 6 2 4 Sample processor 858 inject valve nnsnnnnssnnnnsnrnssrrnesrrnnne 29 20 2 5 Sampie Processor PUM D esensina isnie EAN 31 2 6 2 6 Sample processor 858 peristaltic DUMP ce ecceeeeeeeeeeeeeeeeees 32 2 6 2 7 Sample processor SWING cccccsececceeseecceeseeseeseeesseseeesseseeenees 33 2 6 2 8 Sample processor SUED ssn cuscciessnedotenscncassaueambsiesiecudaisenwwened saseunciis 34 ZF SU Cl COME A E E A E E E 35 ZAA rer manual C OnO lase E 36 2 12 Procedure Sirrer CONO leevn scones tensaese rade eenianehsdxead 38 221 SUMMERS OCC ssrin ni a E EEA ESUE EEEN 39 2 8 Metrohm 6 2148 010 Remote BOX ssnnnsssnnnssnnnssrnnssrrnrsrrresnrnrsrrresneni 40 2 8 1 Procedure Remote Box control ccccccsececeeseeeseeseeeseeseeesseneeesees 40 De Me ROTO LE IOUS acetone E con 42 De Vi MOE OU ee a E 43 2 9 Parallel ODET ATION iss ceansiarsacnusiemnctadnas nende nann EEEa EE EEDE EDE EE
32. Dosino dose delivers the specified volume through the dosing port e Dosino empty empties the dosing cylinder and the tubings e Dosino fill fills the dosing cylinder completely e Dosino to end empties the dosing cylinder completely e Dosino exchange prepares the dosing unit for exchange All the Dosino control commands are tagged as ntermediate commands except the Dosino to end and the Dosino exchange commands that are tagged as Advanced commands Each command requires a device name to be specified The identifying name can be entered directly in the procedure editor or can be selected by clicking the button to display a list of available devices see Figure 14 3 Refer to the Metrohm Dosino user manual for more information 16 Page NOVA External devices tutorial Commands Parameters Links New procedure Remarks z End status Autolab anal Signal sampler Time WE 1 Current m Options No Options ay Instrument Instrument description 2 Dosino prepare Dosino_1 Dosino_1 N Cycles 1 lt gt Device name Please select one item Sample Processor Remote box Stirrer OK X Cancel Figure 14 A list of available devices is displayed in the procedure editor Select the name of the device to control and click the _ button to close the editor The name displayed in the Device name dialog correspond to the names for each device specified in the Liquid Handling Setup 2 5 2 1
33. E 43 ANANE PE OE GIS arae E E T E E 48 3 1 Requirements and installation cccccssscceceeseeeceeseecceeseesseeseessaeeess 48 3A SYNCNTONIZEd MCAS UE CIMIGING 1 ccecicaaceusaateitysiereccussouseinoleaneusanrenetedacacnce 51 BSS COMA O E A E cna devewenaats sean 54 3 3 1 Avantes initialize a5 cscs dassiiesnanadbngs mondsitinsandande sonuiedmadaabagthondauctsahaseadssandeies 55 PV NCS CIOS eE ra E E E aco E E E EEEE 60 33 DSC UM LOO CHING eer R AA E A 63 2 Page NOVA External devices tutorial i SIA ey COM NOE a E E 70 3 4 Avantes firmware update cccccccccccsssscecseeeeeeecseeaeseesseeaeseeseaeeesesseaas 71 4 Analog Control of External AeVICES cee ccccccceceeeeceeeeeeeeeeeeeeeeeseaeeeeeeeeeeaaeeeees 75 4 1 Autolab PGSTAT10 PGSTAT20 PGSTAT30 PGSTAT100 cceeeeee ee 75 4 2 Autolab PGSTAT12 PGSTAT30 302 PGSTAT100 ccecceeeeeeeeeeeeeeeaes 76 4 3 Autolab PGSTAT128N PGSTAT302N PGSTAT302F PGSTAT100N 77 Ae AIPOIA I e E E E E E 77 4 5 PGSTAT101 204 and M101 M204 cc cccceccccceeceeeeeeeeeeeaeeeeesneeeeesneeeeeans 78 4 6 Hardware setup for external GeVICES ccccecccceeseeeeeeeeeeeeeeeeeaeeeeeeeaaneees 78 461 AN O47 VIS SUIS tir cscte ectccencrcenvngnvieacns E vageroneemrennes 79 A 6 2 DACIG4 OUT setings soseen a E E 81 4 7 Measuring external signals saxcscsscosciinissdnascndsarivinxtvbaduesiuwsasirhdpadendacassdeubsnad
34. Fiber diameter AvaSpec HS AvaSpec Avaspec AvaSpec AvaSpec um 1024 2048 3648 NIR256 NIR512 10 O O O 25 O 0 1 1 0 50 0 1 12 2 3 0 0 1 100 12 3 5 6 0 1 12 200 3 4 6 7 12 1 2 3 4 400 7 8 13 14 24 25 2A 7 8 500 9 10 17 31 4 5 9 10 600 11712 21 37 5 6 11 12 Table 1 Optimal smooth pixel settings for the different optical fibre diameters and spectrometer type e Use high resolution ADC enables or disables the high resolution ADC of the spectrometer default enabled When enabled the measured values are resolved using a 16 Bit ADC when disabled a 14 Bit ADC is used instead Vv Avantes Spectrometer setup Basic settings Advanced settings Integration delay 0 ms Dark correction Enable Dark correction value 0 Yo Smoothing model 0 smoothing pixels 0 Use high resolution ADC Enable Cancel Figure 78 Specifying the advanced spectrometer settings Click the button to close the Avantes Spectrometer setup window The specified parameter will be updated in the procedure editor see Figure 79 58 Page NOVA External devices tutorial Avantes initialize Senal number 1105171001 Start wavelength nm 350 Stop wavelength nm 650 Integration time ms 10 Number of averages 1 lt gt Figure 79 The updated procedure parameters Only the basic parameters are indicated in the procedure editor One additional parameter Number of averages can be specified in the procedure editor see F
35. NOVA External Devices Tutorial Version 1 11 0 NOVA External devices tutorial Table of Contents T External CEVICES secre E NES 4 2 Metrohm Serial Bus MSB 0ccccecececcecececceecececeeaecesececaeeeseceeaeaesereveeaeeereees 4 2 1 MIETFONIMGEVICES SUD DOM leors rreri ri nse EE e SEENEN SEET aai 5 2 2 Metrohm drivers installation ccccssccccssseecceeseecceeseeseeseeeseeseeesseneeeseas 5 2 3 Additional requirements ascensaterasatrnsyaecercocncsteruneradeigsaan ations apboiseaconsainainpasnseeaondttetin 6 DF Hardware Se BUN Desni a E hexane saoneceyactas ance nieneaes 7 2 AA Dosim Hardware SOD ae sesetcrscancecosentoiedaraetese te neaceatoncemeancsnemceies 8 2 4 2 Sample processor Hardware SQtup ccccecccesseeeeeeeeeeeeeeeeeeeeeeeeeeaes 10 2 4 3 Stirrer Hardware SQtup ccccccsscecceeseecceseeesceseeeseeseeeseegeeeseeneeeseas 12 2A A CIO OO 6 UD eE E E E E E Ea 13 P a OE OE E A E eee ee ee eee eee 13 2 5 l DOSNO Manual CONTO ast cbeatecuisasnctemuyotedececonsabaniaceieomammuninesmaarce 14 2 5 2 Procedure DoSMorcOnO l sa sssiascscestenctivisteniolndss dciesasteeiensdeseraaditeanianessdxkeasia 16 Za DOSING lO One anara E E O AS 17 2A DOO E E E O E 18 Zi 23 DO NOEM Ve a A E EA E EAE E 20 ASLA D INO Till eee E 20 Laaa DOO O O o E 21 2 5 2 6 DOSING exchange vic sacnccineisenanndabersuncwarancmcinasencnesnesdwonebessendeananenseds 21 2 6 Sampie processor
36. Port B of either one of the DIO connectors located on the instrument A dedicated cable is available for these Autolab instruments preconfigured for the correct digital control the Avantes light source Please refer to Section 5 of this document for more information on TTL triggering 70 Page NOVA External devices tutorial 3 4 Avantes firmware update In order to work in combination with the Autolab the Avantes instruments must be programmed with a specific firmware version firmware 009 028 000 000 or 000 031 000 001 If the firmware version present on the Avantes does not match the required version the firmware needs to be updated This requires the following items e Special application AS5216 Firmware Updater this application provided by Avantes can be downloaded from the Metrohm Autolab website Support page e Firmware file AS5216SW bin the firmware image file also provided by Avantes and downloadable from the Metrohm Autolab website Support page e Interface cable IC DB26 DB9 2 interface cable between the AvaSpec USB2 0 spectrometer DB26 and RS232 DB9 This cable can be obtained from your local Avantes distributor or from Metrohm Autolab Download both files specified above and copy them to a folder on the computer All the downloaded files must be located in the same folder see Figure 93 billig Application Tools Avantes Firmware for Autolab oO EA Home Share View Manage
37. To setup the communication protocol for the external device add the External device initialize command to the procedure see Figure 139 External device initialize L RS232 Device name Device type PERIE Settings 7 entries lt Figure 139 Add the External device initialize to setup the RS232 communication protocol Once the command has been added to the procedure a name for the device must be provided and the Settings of the communication must be defined These settings can be edited by clicking the button see Figure 139 A new window will be displayed allowing the specification of the communication settings see Figure 140 Quick watch Ea FA 4 Wisc BaudRate 4eOU Data Bits Handshake Mone MewLine ri Fart Mone FortName COM stopBits One BaudRate Cancel Figure 140 The RS232 communication settings 103 Page NOVA External devices tutorial The following values need to be defined according to the requirements of the external device e Baud rate Bd e Data bits e Handshake None XOnXOff RequestToSend RequestToSendXOnxXOff e New line n r e Parity None Odd Even Mark Space e Port name COM x is a valid port number e Stop bits The example shown in Figure 140 corresponds to the settings for the Julabo HE F34 water bath 6 2 External device send Once the RS232 communication protocol has been defined and the external device initialized it is possib
38. VA External devices tutorial Example Suppose that the 8 pins of port A of DIO connector 1 must be set to the following sequence see Figure 128 Section OOOOQOQO0O0O0000 Figure 128 Example of a DIO bit sequence The bits for each pin would be written as Pin 8 7 6 5 4 3 2 1 Bit O 1 0 O 1 1 O 1 The corresponding byte would be 01001101 in binary which corresponds to 77 in decimal The trigger sequence shown in this example can be specified in the Autolab control window in binary see Figure 129 or in decimal see Figure 130 95 Page NOVA External devices tutorial have Autolab control 0 PGSTAT302N A DIO PX AAA DIO Section A Jw 125 Summary Section B RE 00000000 i23 Section C w 00000000 Ing BIO PA Figure 129 Writing the trigger sequence in binary have Autolab control 0 PGSTAT302N A DIO PX DIO S Section A Jw em summary Section B R 00000000 log a Section c w 00000000 Ing BIO PZ aaa Figure 130 Writing the trigger sequence in decimal 96 Page NOVA External devices tutorial When the port output is set to the defined bit sequence it will remain unchanged until a new sequence is defined The new sequence can be defined by adding another Autolab contro command to the procedure The PGSTAT101 PGSTAT204 M101 and M204 have a single 8 pin output port on its DIO connector Port A The Autolab control win
39. ace or to a dosing interface with USB Refer to Section 2 for more information 40 Page NOVA External devices tutorial The following Remote Box control commands are available e Remote inputs reads the values of the 8 input lines numbered INO to IN7 of the Remote Box see Figure 55 e Remote outputs sets the values of the 14 output lines numbered OUTO to OUT13 of the Remote Box see Figure 55 7 OUT9 OUT10 8 6 OUTS IN1 9 5 OUTO IN3 10 4 OUT2 IN5 11 3 OUT4 IN7 12 2 OUT7 OUT11 13 1 OUT6 _ jf jf jf ft tT fT ft O OOOO 0 OO O00 OC UOVVVVVVVOIOOO jf jf 0 Volt GND 25 14 0 Volt GND ING 24 15 5 Volt IN4 23 16 OUT5 IN2 22 17 OUT3 INO 21 18 OUT1 OUT13 20 19 OUT12 Figure 55 The pin assignment of the Metrohm 6 2148 010 Remote Box All the Remote Box control commands are tagged as ntermediate commands To use these commands the device name needs to be specified The identifying name can be entered directly in the procedure editor or can be selected by clicking the button to display a list of available devices see Figure 56 2 Refer to the Metrohm 6 2148 101 Remote Box documentation for more information 41 Page NOVA External devices tutorial Commands Parameters Links New procedure Remarks End status Autolab signal sampler Time WE 1 Current Options No Options Instrument Instrument description a
40. an accommodate one or two Swing arms Depending on tower the Robotic arms should be mounted in such a way that each arm can rotate in the correct direction In this Appendix the following identifications referred with respect to the front of the instrument are used see Figure 145 e Tower 1 is located on the right e Tower 2 Is located on the left e A swing direction from left to right is identified as a positive direction e A swing direction from right to left is identified as a negative direction lt ididh Titi Figure 145 Example of a 815 Sample processor fitted with two towers 107 Page NOVA External devices tutorial Presently the following standard Robotic arms are supported see Figure 146 Figure 146 Overview of the supported standard Robotic arms 1 6 1462 030 2 6 1462 040 3 6 1462 050 4 6 1462 060 5 6 1462 070 Table 3 provides an overview of the specifications of the different standard Robotic arms Metrohm code 6 1462 030 6 1462 040 6 1462 050 6 1462 060 6 1462 070 Swing Angle offset 8 8 0 8 8 B 117 117 84 73 73 Swing radius 112 mm 112 mm 110 mm 127 mm 127 mm Swing direction gt z gt Table 3 Overview of the standard Robotic arm settings 108 Page NOVA External devices tutorial Table 4 provides an overview of the specifications of the different advanced Robotic arms Metrohm code 6 1462 080 6 1462 090 6 1462 150 6 1462 1
41. and the position limit specified in the Liquid Handling setup Figure 30 shows an overview of the Sample processor lift command used in a procedure 26 Page NOVA External devices tutorial cl Sample processor litt Sample processor Device name sample processor m Tower 1 _ Postion o SS Figure 30 Using the Sample processor lift command When the command is executed during a procedure the progress of the Lift command can be tracked in the Autolab display panel see Figure 31 Autolab display a v Autolab manual control AUT84311 A sample processor Tower 1 Rack position 1 43 1 1 Mowe Lit position 0 125 5E 125 Move W 5 H Fump Peristaltic pump 0 gt Hold stop 4589 BUSY 000010 Figure 31 The Autolab display indicates the progress of the Lift command 2 6 2 2 Sample processor move This command changes the position of the sample rack relative to the specified Sample processor tower to the required sample position The sample position defined between 1 initial position and x where x is the number of available positions on the rack The rack information is specified in the Liquid Handling setup Figure 32 shows an overview of the Sample processor move command used in a procedure El Sample processor mowe Sample processor Device name sample processor ma Tower 1 Posion R Figure 32 Using the Sample processor move command When the command is executed during a procedure t
42. commands are available in the Measurement External devices group of the command browser see Figure 138 5 External devices Avantes initialize Awantes clase External device initialize 2 External device send s External device receiwe L External device clase Figure 138 The commands related to the control of external devices through RS232 communication are located in the Measurement External devices group External devices can be controlled through RS232 with or without the Autolab potentiostat galvanostat connected to the computer The following commands are available e External device initialize used to setup the connection e External device send used to send a string to the external device e External device receive used to read a string from the external device e External device close used to close the connection The use of the Avantes initialize and Avantes close commands is covered in Section 3 of this document Setting up an RS232 communication protocol can be time consuming It Is recommended to read the reference manual of the external device carefully 102 Page NOVA External devices tutorial 6 1 External device initialize Before the RS232 communication can be used to control an external device the settings of the connection must be defined according to the requirements of the device These settings can be found in the user manual of the external device
43. computer A Warning Make sure that the Metrohm devices are connected to the computer and are switched on before starting the NOVA software 2 3 Additional requirements In order to control the supported Metrohm Sample processors an additional Windows component must be present on the computer The controls for the Metrohm Sample processors use the Microsoft msxml 6 0 DLL ibrary for the configuration files XML file format The msxml6 0 dll may not be preinstalled on every Microsoft operating system 6 Page NOVA External devices tutorial Please ensure the availability of this DLL on the operating If this package is missing please download the installation package from the Microsoft website using the link provided below http www microsoft com en us download details aspx id 3988 2 4 Hardware setup Before the connected devices can be used the hardware settings must be specified After starting NOVA open the Liquid Handling Setup using the Tools menu see Figure 2 File View Profile Run Tools Help leg So ea ba Options Database Manager Check Procedure Alt F1 Harchvare Setup Liquid Handling Setup X pH Calibration l Figure 2 The Liquid Handling Setup is available from the Tools menu The Liquid Handling Setup window will be displayed see Figure 3 7 Page NOVA External devices tutorial Liquid Handling setup 0 4569 656 4589 9126 800 9126 45899
44. devices tutorial Two cables are available see Figure 71 e For the PGSTAT101 PGSTAT204 M101 and M204 this cable is fitted with a 15 pin SUB D connector suitable for the DIO connector of the PGSTAT101 PGSTAT204 or the M101 and M204 module Article code AUT AV TRGCBL DIO1 2 e For all other Autolab instruments this cable is fitted with a 25 pin SUB D connector suitable for one of the DIO connectors of the Autolab Article code AUT AV TRGCBL DIO48 Figure 71 The trigger cables for the PGSTAT101 PGSTAT204 M101 M204 right and other Autolab instruments left The trigger cable is also fitted with an additional connector which can be used to control the shutter of the Avantes UV light source if applicable The trigger cable can be connected to the EXTERNAL I O 15 pin SUB D connector located on the back of the Avantes light source if applicable see Figure 72 Figure 72 The Autolab trigger cable is used to connect to the EXTERNAL I O connector located on the backplane of the Avantes light source Engage the shutter control to TTL on the front panel of the Avantes light source to allow remote shutter control see Figure 73 53 Page NOVA External devices tutorial Figure 73 The shutter control switch must be set to TTL for remote shutter control Q Warning Always use protective eyewear when working with UV light sources 3 3 Avantes control The current version of NOVA supports the Avantes 2048
45. dow will therefore only show a single output sequence see Figure 131 have Autolab control 0 PGSTAT204 Section A 00000000 123 DIO12 Integrator Summary Figure 131 The 8 pins of the write section of the single DIO port of the PSGTAT101 PGSTAT204 M101 and M204 can be set directly from the Autolab control command 97 Page NOVA External devices tutorial 5 3 3 Receiving triggers Before a port can be used to receive a trigger the port must be set to Input mode see Figure 132 and previous section This setting is not available for the PGSTAT101 PGSTAT204 M101 and M204 since the pins of the DIO connector are pre configured to input and output see Section 5 2 Ports A and B are initialized at startup have Autolab control 0 PGSTATSO2ZN Aj DIO P1 ae S Section A m W 00000000 123 summary E Section B R 00000000 2g AA Section C m D 00000000 125 wi DIO P2 Figure 132 Setting a port to Input mode Receiving triggers is done using the dedicated Wat for DIO trigger command available in the Measurement General group see Figure 133 e Wraitfor DIO trigger P1 Port A econ DIO connector F DIO connector port Part A Trigger byte OOOO Time limit 5 z Use time limit No m lt gt Figure 133 The Wait for DIO trigger command can be used to receive a TTL trigger 98 Page NOVA External devices tutorial The Wait for DIO trigger command has the following parameters
46. e DIO connector identifies the connector used to receive the trigger e DIO connector port defines the port used to receive the trigger Port A B C e Trigger byte this is the required byte that must be received in order to proceed A 1 correspond to a high position a O corresponds to a low position and an x is a wildcard e Time limit s this defines the time limit for the previous parameter e Use time limit yes no this defines whether an optional timeout limit is used If a time limit is used the Wait on trigger command will stop waiting when the time limit is exceeded and the pre defined trigger byte has not been received Pressing the button in the procedure editor opens a dialog window which can be used to set the connector and port to be used to receive the trigger see Figure 134 Wait for DIO trigger F1 Port A ae a DIO connector a LIC connector port Fort Trigger byte NOOO Time limitis z Use time limit No a lt gt Figure 134 Opening the Wait for DIO trigger dialog window The Wait for DIO trigger dialog window displays the available DIO connectors and ports that can be used for receiving the TTL trigger The trigger byte and the maximum wait time can also be defined see Figure 135 Wait for DIO trigger DIO connector DIO connector port Trigger byte OOOO _ Time limit 3 5 Ok Cancel Figure 135 The Wait for DIO trigger dialog can be used to def
47. e gt Tower 0 SwingHead Dir lt Tree gt lt Value gt lt Value gt lt TreeNode gt Replace the sign in bold lettering by a or sign depending on swing direction The following convention is used to specify the swing direction e is used when the swing direction is from left to right e is used when the swing direction is right to left e or minus is used when the arm can swing both ways 110 Page NOVA External devices tutorial Swing distance lt TreeNode gt lt Tree gt Tower 0 SwingHead Distance lt Tree gt lt Value gt 166 0000 lt Value gt lt TreeNode gt Replace the value in bold lettering by either 166 or 196 depending on the type of Sample processor e For the 814 and 858 Sample processor the value should be 166 e For the 815 Sample processor the value should be 196 4 Repeat this for tower 2 identified in the xm1 file as Tower 1 if necessary 5 Save the changes When the Sample processor is initialized again the settings for the Swing head and the Robotic arm defined in the modified xm1 file will be used 111 Page
48. e the parallel operation When parallel operation is enabled a Liquid Handling device will not block the procedure while it is executing an action allowing the next command to run and the procedure to continue If this setting is disabled the device will hold the procedure until the action being carried out by the device is finished One additional command Wait for Metrohm device provided in the Metrohm devices group of command can be used to force the procedure to wait for a Metrohm device with the Parallel execution enabled see Figure 60 44 Page NOVA External devices tutorial E Metrohm devices Dosino prepare be Dosino dose Dosino empty Dosino fill Dosino to end Dosino exchange Sample processor lift 7 Sample processor move Sample processor valve Sample processor 656 inject valve z Sample processor pump Sample processor 658 peristaltic pump Figure 60 The Wait for Metrohm device command is available in the Metrohm devices group of commands To illustrate the parallel operation option four examples are shown in Figure 61 In this figure the duration of the Dose commands is shorter for Dosino 1 than for Dosino 2 45 Page NOVA External devices tutorial Dosino 1 Dose Dosino 2 Dose CV Staircase A Time Dosino 1 Dose Dosino 2 Dose B Tine CV Staircase Dosino 1 Dose CV Staircase C Time Dosino 2 Dose Dosino 1 2 Dose D CV Staircase Figure 61 Experiment flow for f
49. etrohmSamplerConfig xsd gt lt TreeNode gt lt Tree gt Tower 0 Lift Max lt Tree gt lt Value gt 235 lt Value gt lt TreeNode gt lt TreeNode gt lt Tree gt Tower 0 Lift Radius lt Tree gt lt Value gt off lt Value gt lt TreeNode gt lt TreeNode gt lt Tree gt Tower Lift RotiIncr lt Tree gt lt Value gt 5 000 lt Value gt lt TreeNode gt lt TreeNode gt Figure 147 Open the Config xml file with a text editor 109 Page NOVA External devices tutorial 3 Inthe xml file locate the following items Swing Angle offset lt TreeNode gt lt Tree gt Tower 0 SwingHead Offset lt Tree gt lt Value gt 0 0000 lt Value gt lt TreeNode gt Replace the value in bold lettering by the value listed in Table 3 or Table 4 for the Robotic arm used with the Swing head If the value is negative specify this value with the sign Max swing angle range lt TreeNode gt lt Tree gt Tower 0 SwingHead MaxRange lt Tree gt lt Value gt 60 0000 lt Value gt lt TreeNode gt Replace the value in bold lettering by the value listed in Table 3 or Table 4 for the Robotic arm used with the Swing head Swing radius lt TreeNode gt lt Tree gt Tower 0 SwingHead Radius lt Tree gt lt Value gt 110 0000 lt Value gt lt TreeNode gt Replace the value in bold lettering by the value listed in Table 3 or Table 4 for the Robotic arm used with the Swing head Swing direction lt TreeNode gt lt Tre
50. four ports of the Metrohm 800 Dosino VENT Port 1 Port 3 Port 4 Port 2 Figure 5 Overview of the ports located on the dosing unit 2 4 2 Sample processor Hardware setup The Liquid Handling setup window can be used to specify the settings for the Metrohm 814 815 or 858 Sample processor shown in Figure 6 for the Metrohm 858 Professional Sample Processor 10 Page Name Rack type Tower 1 settings Active Lift rate 3 mm s 25 mm s Shift rate 3 s 20 s owing rate 10 s 55 s Work position 0mm 235mm Position limit OQmm 235mm 4589 62041360 10 100 100 3 3 10 100 100 NOVA External devices tutorial Parallel execution Pumps Valves Stirrer Peristaltic pump Injection valve Figure 6 Overview of the settings and parameters for a Metrohm 858 Professional Sample Processor The following settings can be specified see Figure 6 e Name of the device a unique name to identify the instrument in NOVA By default the name of the device corresponds to the serial number of the device until a user defined name is assigned to the device e Parallel execution specifies if the parallel execution is allowed for this device refer to Section 2 9 for more information e Rack type the rack used on the sample processor The rack code is specified on the rack itself see Figure 7 11 Page NOVA External devices tutorial 2 Metrohm 6 2041 3
51. he device a unique name to identify the instrument in NOVA By default the name of the device corresponds to the serial number of the device until a user defined name Is assigned to the device The Metrohm 6 2148 010 Remote Box is identified as a 770 device type 2 5 Dosino control The current version of NOVA supports the Metrohm 800 Dosino through the MSB interface see Figure 10 Metrohm devices with MSB connectors cannot be connected to the computer directly These devices must be connected to a Metrohm instrument with USB interface or to a dosing interface with USB Refer to Section 2 for more information 13 Page NOVA External devices tutorial Figure 10 Metrohm Dosino fitted with a 10 ml burette The Dosino can be controlled in two different ways e Manually using the dedicated controls e During a NOVA procedure using the provided commands 2 5 1 Dosino manual control To access the manual control panel of a connected Dosino select the device from the Liquid Handling manual control in the view menu see Figure 11 14 Page NOVA External devices tutorial File View Profile Run Tools Help Advanced procedure view T7 es es dEl E va 9 Ca z Setup View Multi Autolab View Measurement View Analysis View User log F11 Autolab display F10 FRA manual control i B E MDE manual control MUX manual control External manual control Liquid Handling manual control Sample Processor 658
52. he progress of the Work command can be tracked in the Autolab display panel see Figure 33 27 Page NOVA External devices tutorial Autolab display EJ Autolab manual control AUT84311 A sample processor Tower 1 Rack position 1 46 0 de Move Lift position 0 125 0 T25 Move 5 H Fump Peristaltic pump 0 gt 4589 BUSY 000010 Figure 33 The Autolab display indicates the progress of the Lift command Clicking the button will stop the Move command When the sample rack is fitted with several rows of samples and the sample processor is fitted with a swing arm the swing arm will be controlled during the Sample processor move command in order to reach the specified position When the sample processor lift is not in the shift position 0 mm the lift will be first moved to the shift position before the rack is moved to the specified position 2 6 2 3 Sample processor valve This command controls the optional valves of the specified tower These valves when installed are located on the back of the sample processor tower see Figure 34 28 Page NOVA External devices tutorial Valve 2 Valve 1 Valve 1 Figure 34 Location of the valves left one valve right two valves Figure 35 shows an overview of the Sample processor valve command used in a procedure El Sample processorvalve Sample processor Device name sample processor ma Tower 1 0 Sa Figure 35
53. hm device Device Device T lt gt Device name Please select one item sample processor stirrer a Figure 62 Using the Wait for Metrohm device command Note The Wait for Metrohm device command has no effect on devices for which parallel operation s disabled 47 Page NOVA External devices tutorial 3 Avantes Spectrometers Avantes spectrometers can be controlled through a USB connection in combination with NOVA The AvaSpec 2048 and AvaSpec 3648 USB 2 0 Avantes spectrometers are currently supported see Figure 63 AVANTES D Starline AvaSpec ULS3648 Power Scan Figure 63 Avantes spectrometers are integrated in NOVA Through the provided support it is possible to setup the spectrometer and to synchronize the acquisition of spectra with electrochemical measurements in NOVA 3 1 Requirements and installation Before connecting the Avantes spectrometer to the computer make sure that the AvaSoft is installed on the computer This software is supplied with the instrument During the installation of the AvaSoft the Driver Installation Wizard will be displayed see Figure 64 48 Page NOVA External devices tutorial Device Driver Installaton Wizard Welcome to the Device Driver Installation Wizard This wizard helps you install the software drivers that some computers devices need in order to work To continue click Mest Figure 64 Installation of
54. igure 115 85 Page NOVA External devices tutorial File View Profile Run Tools Help be Advanced procedure view i T ee go Jel El a cw a Setup View Multi Autolab View Measurement View ia Analysis View J User log F11 Ea Autolab display F10 FRA manual control MDE manual control MUX manual control External manual control Add remove External manual control panel in Autolab display Figure 115 Select the External manual control option from the View menu to add the control panel to the Autolab display The Autolab display will be shown with an additional panel for the control of the external device see Figure 116 Autolab display x a Autolab manual control AUT83478 HS TAB status current range Q Extemal device control Autolab LED Driver A Figure 116 Manual control of external devices is provided by a dedicated extension panel of the Autolab display 86 Page NOVA External devices tutorial The external device signal name and units are shown in the External device control panel To specify a value click the name and type the required value The value can be validated by pressing the Enter key on the keyboard see Figure 117 Autolab display x Autolab manual control AUT834 3 A External device contral Autolab LED Driver A Autolab display x Autolab manual control AUT83478 a External device control Autolab LED Driver A Figure 117 C
55. igure 79 This parameter can be 1 or higher However values higher than 1 are only available when the Avantes firmware used by the spectrometer is version 000 031 000 001 To trigger the acquisition of the spectra on the AvaSpec a dedicated trigger cable is required see Section 3 2 This cable is used to connect the EXTERNAL I O 27 pin SUB D connector located on the back of the Avantes spectrometer to one of the available DIO ports on the Autolab During the measurement while the spectrometer is acquiring data the Scan LED on the front panel of the spectrometer will be lit see Figure 80 AvaSpec 2048 Figure 80 The Scan LED is lit during measurements All the measured data points are stored in the on board memory of the Avantes spectrometer until the control of the instrument is released with the Avantes close command see Section 3 3 2 59 Page NOVA External devices tutorial 3 3 2 Avantes close To close the control of an initialized Avantes spectrometer the Avantes close command is used see Figure 81 This command is also used to recover the spectral data stored in the on board memory of the Avantes spectrometer Seral number Input reference signal lt arrary gt Input reference signal step 1 spectrum number lt arrary gt Wavelenqth lt array gt nm Measured value lt Array au Output reference signal lt arrary gt Figure 81 The Avantes close command The Avantes close ca
56. ikusul PLZ164WA electronic load DAC164 1 Signal name Kikusui PLZ TAWA n Signal unit Conversion slope 3 3 Conversion offset 0 Upper limit 0 Lower limit Figure 111 Defining and saving the settings for a custom signal Kikusui PLZ164WA Once the settings are saved in the presets they can be reloaded at any time using the provided drop down list see Figure 112 83 Page NOVA External devices tutorial out Signal name Kikusut PLZT bya CC Hi ix l l Autolab LED Oriver aoned Autolab RDE dkusul PLZT EJA CC Conversion slope Ln TEALA AYN Conversion offset 0 A Upper limit 33 A Lower limit 0 Ay Figure 112 Saved presets are available from the drop down list It is also possible to delete a saved preset by clicking the button located next to the drop down list see Figure 113 DAC164 1 Signal name Kikusul PLA TB4WA w Signal unit Conversion slope 3 3 Conversion offset 0 Upper limit 0 Default presets shown in blue cannot be deleted Figure 113 Deleting a saved preset 4 7 Measuring external signals Once the settings have been defined in the Hardware setup as explained in Section 4 6 1 the signal from the external device can be recorded like any other electrochemical signal through the defined ADC input ADC164 or Vin To measure external values during a measurement the signal sampler must be adjusted see Figure 114 The signals defined in the Hardware setup are
57. ine the command parameters 99 Page NOVA External devices tutorial For the PGSTAT101 PGSTAT204 M101 and M204 module a single input port is available on the DIO connector see Figure 136 Wait for DIO trigger DIO connector DIO connector port Trigger byte OON _ Time limit 3 5 Cancel Figure 136 A single DIO connector is available for the PGSTAT101 PGSTAT204 M101 and M204 module Note The trigger byte consists of 4 bits for the PGSTAT101 PGSTAT204 M101 and M204 The trigger byte that should be received is defined in the Wait on DIO trigger command using an 8 character string containing 1 high O low and x wild card Each character in the string corresponds to a pin on the DIO port from right to left For example if the measurement should be allowed to proceed when the following trigger byte is received Pin 8 7 6 5 4 3 2 1 Bit 1 O 1 1 O0 O 1 O The corresponding byte would be 10110010 in binary If this Trigger byte is defined in the Wart on DIO trigger command the measurement will be allowed to proceed only when pins 2 5 6 and 8 are set to high position AND pins 1 3 4 and 7 are set to low position 1 gt Or 4 characters for the PGSTAT101 PGSTAT204 and M101 100 Page NOVA External devices tutorial If the status of pins 1 2 and 3 are irrelevant the wildcard symbol x can be used Pin 8 7 6 5 4 3 2 1 Bt 141 0 1 1 0 x X xX The corresponding byte would be 10110
58. ited 84 4 8 Sending external signals ccccccccssseseecceesseeeceeeseeeccseeseeesseesseeessaagees 85 4 8 1 Manual control of the DAC164 VoOUt ec eeecccce ee eeeeeeeeeeeeeeeeees 85 4 8 2 Procedure control of the DAC164 VoOUt ee ccccecccceeeeeeeeeeeeaeeeeenes 87 BT Fe POSES DIO act teateeeni cece casige i scetine oeeso ns ence ae dcaonepiecemaeseacom snsndsteusanacen 89 5 1 Autolab PGSTAT series 7 and series 8 and uAutolab III eee 89 5 2 Autolab PGSTAT101 PGSTAT204 M101 and M204 cceccccceeeeee eens 90 Bs NOEN cetacean ttm encarta acs taste ap momma EE EAEE 91 Go ol POPE Ila Zeall ON a E E 91 Big DNC OSI S a E E N 92 BO RECEIVING UIC OCI raor N 98 6 Recommended Standard 232 RS232 cccccccecececcececececcecececuceeeerecueeeeeeees 102 6 1 External device initialiZe ccccccsececcesececceseeeceeseeeceeseeecseneeessageeeseaaes 103 2 Exlermnal device Send assets cred seers gestern EN 104 60 3 ExXlernal GEViICE TECIE ee aneenee een an ne ee ence nee eee eee 105 6 4 External device close 2 6 ccsccescesseseceedseeeesadeseccsedeeesseedensnssedesneseedeneess 106 3 Page NOVA External devices tutorial 1 External devices In many electrochemical experiments it is often required to control external devices like stirrers water baths sample changers etc While most of these external devices can be controlled directly using dedicated hardware o
59. lab control window see Figure 125 92 Page NOVA External devices tutorial Autolab control 0 PGSTAT302N A DIO P1 ae S section A w 00000000 123 summary SsectionB R 00000000 23 AA Section C w 00000000 123 w DIO P2 Figure 125 The Autolab control window is used to set the DIO port The following syntax is used to define a trigger bit sequence Each pin of a port corresponds to one bit Each pin can be set to Low 0 or High 1 status and the sequence of 8 pins defines the trigger bit sequence The trigger sequence can be written in decimal or in binary e Binary the sequence is written as a series of 8 values 0 or 1 e Decimal the value is written as an integer O 0 to 255 The default settings of the Autolab control window allow the value to be entered as binary To switch to decimal click the button located next to the input field see Figure 126 93 Page NOVA External devices tutorial avy Autolab control PGSTAT3S02N AJ DIO P1 pio scion A w m fs summary Figure 126 It is possible to switch from binary to decimal by clicking the s button To revert to binary click the button located next to the input field see Figure 127 hav Autolab control PGSTAT302N A DIO P1 DIO Section A Jw 0 Summary Section B Section w 00000000 w DIO P2 Figure 127 It is possible to switch from decimal to binary by clicking 3 button 94 Page NO
60. le to send data strings to the device for control purposes To send a string to the external device add the External device send command to the procedure see Figure 141 E External device initialize Julabo R5232 Device name Julabo Device type Farii ca settings r entries m e External device send Julabo out_sp_00 24 0 Device name Julabo Command out_sp_00 29 0 gt Figure 141 The External device send command can be used to send a control string to the external device The example shown in Figure 141 is an example of a control string used to set the temperature control of the Julabo HE F34 water bath to 29 C The syntax of the control string is specific for this device More information about the communication syntax can usually be found in the user manual of the external device The Device name must be the same as the one defined in the External device initialize command To avoid errors it is possible to link the Device name parameter of an External device command to the same parameter in the External device send command as shown in Figure 141 It is possible to create a linkable parameter in the data string This is very convenient because it allows combination between the External device send command and other NOVA commands Figure 142 shows an example of a link between an Input box parameter and the temperature parameter in the control string 104 Page i External device initialize Device name Device type
61. length nm 250 Stop wavelength nm 600 Integration time ms 500 Number of averages 1 Autolab control err Set potential 0 000 Set cell On I Wait time s nt Optimize current range 5 E CV staircase 0 000 1 000 1 000 0 000 2 0 1000000 Start potential V 0 000 Upper vertex potential V 1 000 Lower vertex potential V 1 000 Stop potential V 0 000 Number of stop crossings 2 Step potential V 0 00244 Scan rate V s 0 1000000 Estimated number of points 1650 Interval time s 0 024400 signal sampler Time WE 1 Potential WE 1 Current ma Options 1 Options Potential applied array gt V Time lt _ array gt s WE 1 Current lt _array gt A Scan lt _amay gt WE 1 Potential lt _ armay gt V Index lt _ array gt ivs E aay Set cell Off Tii E Avantes close Seral number 1105110U1 Input reference signal Potential applied v Input reference signal step 5 Spectrum number lt _amray gt Wavelength lt _ array gt nm Measured value lt _amay gt a u Output reference signal lt aray Measured value vs wavelength E X Wavelength nm Y Measured value a u ra spectrum number show during measurement Yes ca Measurement plot number 2 ca ZLA Figure 83 Example of a measurement procedure with the Avantes spectrometer 62 Page NOVA External devices tutorial If an electrochemical signal is linked to the Input reference signal parameter of the Ava
62. length of the measurement segment in nm This value must be larger than the Start wavelength and should fit within the measurable range of the spectrometer e Integration time ms the integration time used to acquire each spectrum in milliseconds 56 Page NOVA External devices tutorial Vv Avantes Spectrometer setup Basic settings Advanced settings Seral number 1105170U1 start wavelength nm stop wavelength 650 nm Integration time ms OK X Cancel Figure 77 Specifying the basic spectrometer settings The following advanced settings can be defined on the Advanced settings tab of the Avantes Spectrometer setup window see Figure 78 e Integration delay the integration delay in milliseconds e Dark correction enables or disables the dark correction e Dark correction value specifies the value of dark correction 0 100 e Smoothing model specifies the smoothing model used if applicable Presently only one model is supported and this parameter must always be set to 0 e Smoothing pixels the number of pixels used in the smoothing algorithm When this value is set to 0 no smoothing is used The optimal value depends on the fiber diameter and the pixel size which depends on the type of Spectrometer see Table 1 14 For a complete description of the Advanced settings of the Avantes spectrometer the reader is invited to refer to the AvaSpec user manual 57 Page NOVA External devices tutorial
63. lick the name of the external device signal to edit the value Pressing the Enter key validates the specified value see Figure 118 Autolab display x Autolab manual control AUT83478 A External device control Autolab LED Driver A Litre Figure 118 The specified value is applied on the external device 4 8 2 Procedure control of the DAC164 Vout Control of the external device can also be performed at any time during a procedure using the Contro external device command This command can be found in the Measurement General group of commands The command can be added anywhere in the procedure see Figure 119 87 Page NOVA External devices tutorial Control extemal device Sar Autolab LED Driver Value 0 lt gt Figure 119 The Control external device command The Contro external device command displays the name of the external device specified in the hardware setup and the units of the signal The value can be specified directly in these units To adjust the value click the button located in the procedure editor as shown in Figure 119 An Autolab control window will be displayed The value for the external signal can be adjusted on the Advanced section of the instrument control panel see Figure 120 ii Autolab LED Driver Value 0 have Autolab control 0 J PGSTAT302N lt v Basic DIO AJ Advanced SUUE External input Off Oscillation protection On Reference poten
64. ll interrupt the whole dosing command and continue with the next command in the procedure It is possible to add a link between the volume used in the Dosino dose command and another NOVA command like an Input box see Figure 20 19 Page NOVA External devices tutorial E Input box Title of box Dose Message volume to dose in ml Time limit s a0 lse time limit No maa E Dasino dose Analyte 20 1 Device name Analyte Volume mil Fort 1 lt gt Figure 20 Linking the Dosino dose command to an Input box If the requested volume is larger than the volume of the dosing cylinder fitted on the Dosino the dosing cylinder will be refilled several times until the requested volume is dispensed 2 5 2 3 Dosino empty With this command the tubing system and Dosing unit cylinder can be completely emptied The liquid in the dosing cylinder is ejected via the dosing port specified in the Liquid Handling setup The air required to displace the liquid from the tubing is aspirated via the vent This command performs the opposite as the Dosino prepare command Figure 21 shows how to use the Dosino empty command Dosino empty Analyte Device name Analyte a gt Figure 21 The Dosino empty command Like the previous commands the progress of the Dosino Empty command is indicated the Autolab display 2 5 2 4 Dosino fill This command can be used to fill the dosing unit cylinder complete
65. ly The liquid is aspirated via the fill port Figure 22 shows how to use the Dosino fill command 20 Page NOVA External devices tutorial Dosino fill Analyte Device name Analyte Tr gt Figure 22 The Dosino fill command 2 5 2 5 Dosino to end With this command the content of the dosing cylinder is ejected via the specified port The piston stops at the specified end volume This is useful for pipetting functions or for removing air bubbles from the dosing cylinder Figure 23 shows how to use the Dosino to end command Dosino to end Analyte 1 Device name Analyte a Fort 1 4 Figure 23 Using the Dosino to end command 2 5 2 6 Dosino exchange Before changing the dosing unit the Dosino exchange command must be used to fill the dosing cylinder and move the stopcock to the exchange position The cylinder is filled by aspirating the necessary volume via the specified port Figure 24 shows how to use the Dosino exchange command Dosino exchange Device name Analyte gt Figure 24 The Dosino exchange command 21 Page NOVA External devices tutorial 2 6 Sample processor control The current version of NOVA supports the Metrohm 814 815 and 858 Sample processors through the USB interface see Figure 25 a Figure 25 Metrohm 814 left 1 tower and Metrohm 815 right 2 towers fitted with the Metrohm 786 Swing head sample processors The Sample processor can be cont
66. mp Remote inputs DigitallO_1 w Inputs lt gt Device name Please select one item sample Processor Analyte Remote box Cancel Figure 56 A list of available devices is displayed in the procedure editor Select the name of the device to control and click the __ button to close the editor The name displayed in the Device name dialog correspond to the names for each device specified in the Liquid Handling Setup 2 8 1 1 Remote inputs This command can be used to read the state of the 8 input lines numbered INO to IN7 The state of each input line can be either low or high represented by a 0 or a 1 respectively The state of the 8 input lines is provided as a 8 character string consisting of 0 and 1 representing the state of the input lines from IN7 to INO see Figure 57 42 Page NOVA External devices tutorial Remote inputs Device name Remote box Inputs lt gt Figure 57 Using the Remote inputs command The Input parameter shown in Figure 57 can be linked to a Message Box command to report the state of the Input lines The reported stated of the 8 input lines corresponds to the state of these input lines upon execution of the Remote inputs command 2 8 1 2 Remote outputs This command can be used to read the state of the 14 output lines numbered OUTO to OUT13 The state of each output line can set to either low or high
67. n be located anywhere in the procedure The Avantes close command requires the serial number of spectrometer to close to be specified in the Serial number field It is only possible to close an initialized spectrometer For convenience sake it is possible to link the Serial number parameter of the Avantes close command to the Serial number parameter of the Avantes initialize command Additionally the Avantes close command provides a link to an input signal that can be used to match the spectroscopic data to the electrochemical data Since the memory of the Avantes instrument is limited to a maximum of 1013 scans of 2048 points it is common practice to trigger the acquisition of a spectrum every X data points during the experiment Figure 82 shows an example of such a strategy Here a TTL pulse is sent to the Spectrometer every 9 data points At the end of the measurement it is possible to correlate the data coming from the Avantes spectrometer to the signal leading the electrochemical experiment the Potential applied in the example of Figure 82 60 Page NOVA External devices tutorial TIL pulses Cyclic voltammetry E vs index Potential applied W DIO output ae 20 AQ BU S50 100 120 140 160 Figure 82 An example of TTL pulse sequenced every 9 points during a cyclic voltammetry experiment blue curve potential applied versus point index red curve TTL pulses used to trigger the acquisition of the spectra The
68. n be selected from a list of predefined settings using the Signal name drop down list or can be defined for a specific device It is possible to save the defined settings by specifying the following parameters Signal name the name used to identify the external signal Signal unit the units of the external signal Conversion slope the conversion of voltage to specified units of the external signal Conversion offset the offset for the conversion of the voltage to units of the external signal Upper limit the highest allowed value for the external signal Lower limit the lowest allowed value for the external signal To use the upper and lower limit the checkbox needs to be checked 82 Page NOVA External devices tutorial For example for the Autolab RDE available as a default from the drop down list the settings are the following see Figure 110 e Signal name Autolab LED Driver e Signal unit A e Conversion slope 0 1 A V e Conversion offset 0 A e Upper limit 0 7 A e Lower limit O A DAC164 1 Signal name Fe signal unit A Conversion slope 0 1 AV Conversion offset 0 Upper limit 0 7 A Lower limit 0 Figure 110 The default settings for the Autolab LED Driver It is possible to define parameters for custom external signals and save them by clicking the 4 button next to the Signal name parameter For example the settings shown in Figure 111 correspond to the constant current control of a K
69. nal devices tutorial Figure 47 Metrohm 801 Magnetic stirrer The Magnetic stirrer can be controlled in two different ways e Manually using the dedicated controls e During a NOVA procedure using the provided commands 2 7 1 Stirrer manual control To access the manual control panel of a connected Stirrer select the device from the Liquid Handling manual control in the view menu see Figure 48 36 Page File View Profile Run Tools Help Advanced procedure view Setup View Multi Autolab View Measurement View Analysis View F11 F10 User log A ar Autolab display FRA manual control MDE manual control MUX manual control External manual control Liquid Handling manual control NOVA External devices tutorial Hy eo cs fel E T Cu TE sample Processor 858 4589 Analyte 800 9128 Remote box 770 45892 Stirrer 801 2358 N Figure 48 Manual control of the Liquid Handling devices is available from the View menu Connected devices are identified as Name Metrohm Device type Serial Number The Autolab display will be displayed with an additional panel for the selected instrument see Figure 49 Autolab display x Stirrer Speed 0 g lt gt 2358 o Figure 49 Manual control of a Stirrer The Stirrer manual control panel provides the following controls e Control of the rotation speed through the numeric up down control and
70. nates the Sample processor control All the Sample processor control commands are tagged as ntermediate commands except the Sample processor valve Sample processor pump and the Sample processor swing commands that are tagged as Advanced commands Each command requires a device name to be specified The identifying name can be entered directly in the procedure editor or can be selected by clicking the button to display a list of available devices see Figure 29 4 Refer to the Metrohm Sample processor user manual for more information 25 Page NOVA External devices tutorial Commands Parameters Links New procedure Remarks ma End status Autolab z Signal sampler Time WE 1 Current ma Options No Options ma Instrument Instrument description amp Sample processor lift SampleProcessor_1 SampleProcessor_ 1 w Tower 1 Postion 0 gt Device name Please select one item Sample Processor Analyte Remote box Stirrer Cancel Figure 29 A list of available devices is displayed in the procedure editor Select the name of the device to control and click the button to close the editor The name displayed in the Device name dialog correspond to the names for each device specified in the Liquid Handling Setup 2 6 2 1 Sample processor lift This command changes the position of the lift on the specified Sample processor tower The position of the lift can be defined between O mm top of the tower
71. nce the settings are saved in the presets they can be reloaded at any time using the provided drop down list see Figure 107 ADC164 1 signal name Kikusul PLATO A Hi ix l i Autolab Default Signal unit Autolab LED Driver Kikusul PLZ 164WA Conversion slope Conversion offset 0 A Figure 107 Saved presets are available from the drop down list It is also possible to delete a saved preset by clicking the button located next to the drop down list see Figure 108 ADC164 1 Signal name Kikusul PLZ 1b4WiA W Bacal Delete from presets Conversion slope 3 3 AM Conversion offset 0 A Figure 108 Deleting a saved preset Default presets shown in blue cannot be deleted The user defined settings are stored in Externa UserPresets xm file located the ProgramData Metrohm Auto lab 77 0 folder It is possible to copy this file to other computers to duplicate these settings 4 6 2 DAC164 Vout settings The settings for the DAC164 output or the Vout output depending on the type of instrument can be defined in the dedicated panel on the right hand side of the Hardware setup window see Figure 109 81 Page NOVA External devices tutorial DAC164 1 Signal name z Signal unit Conversion slope 1000 RPM V Conversion offset 0 RPM Upper limit 10000 RPM Lower limit 0 RPM Figure 109 The settings for the DAC164 Vout are defined in a dedicated panel in the Hardware setup The settings ca
72. ntes close command and if an Input reference signal step is provided the Output reference signal will be added to the spectroscopic data This signal will contain every n point of the Input reference signal where n is the value specified for the Input reference signal step parameter Figure 84 shows an example of spectroelectrochemical data obtained using a procedure similar to that of Figure 83 The overlay consists of a series of spectra recorded at different potential values during a linear sweep voltammetry experiment Absorbance 250 300 350 400 450 500 550 600 650 700 750 Wavelenght nm Figure 84 Example of spectroelectrochemical measurements 3 3 3 Spectrum triggering To trigger the acquisition of a single spectrum on an initialized Avantes Spectrometer a TTL pulse needs to be sent by the Autolab to the spectrometer Depending on the type of Autolab used the triggers are sent by different pins 1 For the PGSTAT101 PGSTAT204 M101 and M204 module the TTL trigger is sent by pin 1 on the DIO12 connector with respect to the digital ground embedded into the DIO connector A dedicated cable is available for these Autolab instruments preconfigured for the correct digital control of the Avantes spectrometer 2 For all the other Autolab instruments except the PGSTAT302F the TTL trigger is sent by pin 1 of Port A of either one of the DIO connectors located on the instrument A dedicated cable is available for these A
73. ohm autolab com for more information 3 2 Synchronized measurements When used in combination with the Autolab the Avantes spectrometer can be triggered to acquire one or more spectra within the measurable range The acquisition of a spectrum is triggered by sending a TTL pulse from the Autolab to the Avantes spectrometer Each pulse triggers the acquisition of one spectrum Since the Autolab can be programmed to send triggers at any time during a measurement it is possible to synchronize electrochemical data with spectroscopic data see Figure 69 A Warning The PGSTAT302F cannot be connected to the Avantes spectrometers 51 Page NOVA External devices tutorial Measurement time Reset Action l l l l l l l l l l l l l l l l l l l FS Reset Action l l l l Initial potential i l l l l t Interval time i Point i 2 4 s oe 7 Counter 1 2 o 1 2 o 1 Figure 69 Executing the option list every 3 points To trigger the acquisition of the spectra on the AvaSpec a dedicated trigger cable is required This cable is used to connect the EXTERNAL I O 27 pin SUB D connector located on the back of the Avantes spectrometer to one of the available DIO ports on the Autolab see Figure 70 Figure 70 The Autolab trigger cable is used to connect to the EXTERNAL I O connector located on the backplane of the Avantes spectrometer 52 Page NOVA External
74. onnector In the software is it possible to address each of the 24 available pins by port Each port can be set to write or read This provides a convenient way of sending or receiving a predefined digital TTL trigger to or from another device in order to synchronize measurements or to control events during a measurement The TTL 89 Page NOVA External devices tutorial triggers must be set according to the communication protocols defined in user manual of the ancillary device connected to the DIO connector on the Autolab There is a risk of introducing a ground loop when connecting an external device to the Autolab This can result in a higher than expect noise level during the measurements A Warning The DIO ports of the PGSTAT302F cannot be used to connect to external devices 5 2 Autolab PGSTAT101 PGSTAT204 M101 and M204 The PGSTAT101 PGSTAT204 M101 and M204 are fitted with a single non configurable DIO connector located on the back plane of the instrument The DIO connector has a total of eight write lines and four read lines see Figure 123 5 OUT2 6 OUT4 alesis 4 Port B oie 1 IN1 3 Isolated GND fA oo vdovo oe PPPPMAE A E E SNS f f f f f XN Port A 9 IN2 15 OUT7 11 o i 14 OUT5 DGND 13 OUT3 12 OUT1 Figure 123 Mapping of the PGSTAT101 PGSTAT204 M101 and M204 DIO connector Port A includes 8 write lines and two digital ground pins pin 4
75. our different Liquid handling configurations e Figure 61 A Dosino 1 and Dosino 2 have parallel execution disabled Both Dosino need to finish the Dose command before the CV staircase command can start e Figure 61 B parallel execution is enabled on Dosino 2 and disabled on Dosino 1 Dosino 2 starts dosing immediately after Dosino 1 is finished The CV staircase command starts as soon as Dosino 2 starts dosing e Figure 61 C parallel execution is enabled on Dosino 1 and disabled on Dosino 2 Dosino 2 starts dosing at the same time as Dosino 1 Only when Dosino 2 is finished can the CV staircase command start e Figure 61 D parallel execution is enabled for both Dosino 1 and Dosino 2 All three commands start at the same time The Wait for Metrohm device command can be used in a procedure to force the procedure to wait until the specified device finishes the command it is executing This command can thus be used to overrule the parallel operation of the device To use the command drag it into the procedure editor at the location where the procedure must wait and specify the device name using the provided dialog see Figure 62 46 Page NOVA External devices tutorial Commands Parameters Links New procedure Remarks z End status Autolab aa oignal sampler Time WWE 1 Current m Options Mo Options aa Instrument Instrument description E Dosino dose Analyte 0 2 Device name Analyte m olume mij 0 Fort 2 Waitfor Metro
76. r software it Is convenient to allow NOVA to control these devices directly during an electrochemical experiment NOVA supports a number of communication protocols which can be used to control external devices The following external devices are supported e Metrohm liquid handling devices through the dedicated Metrohm Serial Bus MSB e Avantes spectrometers through USB Additionally the following generic communication protocols are available e Analog control e TTL triggers DIO e Recommended Standard 232 RS232 Scope of the tutorial The aim of this tutorial is to explain how to setup and use external devices in NOVA Particular attention is given to Metrohm and Avantes devices compatible with this version of the software Analog control DIO triggering and the RS232 protocol is also explained at the end of the tutorial 2 Metrohm Serial Bus MSB The MSB interface is the standard interface for Metrohm dosing devices sample processors and Stirrers It is present on most of the current Metrohm devices Please note that the Autolab PGSTAT does not have MSB ports that can be used to control MSB devices Instead a USB controlled Metrohm device providing MSB ports must be used see next Section for more information 4 Page NOVA External devices tutorial 2 1 Metrohm devices support This version of NOVA provides support of the following Metrohm devices e 800 Dosino e 801 Magnetic stirrer e 802 Rod stir
77. re 67 The Power LED on the front panel is lit when the instrument is connected and powered In order to work in combination with NOVA the Avantes spectrometer must be fitted with a dedicated firmware that allows remote triggering of data acquisition from the Autolab Two firmware versions are available e Version 009 028 000 000 which supports all available functions of the Avantes spectrometer except signal averaging e Version 000 031 000 001 which supports all available functions of the Avantes spectrometer including signal averaging This is the recommended firmware version 50 Page NOVA External devices tutorial The firmware version can be read from the User log in NOVA see Figure 68 User log message Time Date i Avantes USB connected 1105110U1 Version 000 031 000 001 000 010 001 000 2 1544PM 10 21 2014 1 Autolab USB connected AUT83478 21555PM 10 21 2014 Figure 68 The serial number and firmware version of the detected AvaSpec instruments is indicated in the User log of NOVA Note Two firmware versions are listed in the user log see Figure 68 This first series of 12 digits is the spectrometer firmware the second series refers to the FPGA firmware of the spectrometer Only the spectrometer firmware must be verified If required the firmware needs to be reprogrammed see Section 3 4 This requires a dedicated serial to Avantes spectrometer cable Contact your Metrohm Autolab distributor or info metr
78. rer e 803 Titration stand with stirrer and pump e 814 USB Sample processor e 815 Robotic USB sample processor e 846 Dosing interface e 858 Professional sample processor e 786 Swing head e 6 2148 010 MSB Remote box The instruments indicated in bold lettering in the list above are controlled through USB and provide MSB ports that can be used to control other supported Metrohm instruments Metrohm devices can be controlled in NOVA with or without the Autolab connected to the computer 2 2 Metrohm drivers installation In order to control the supported Metrohm devices the required Metrohm USB drivers must be correctly installed on the computer The driver installation package can be downloaded from the Metrohm Autolab website Support page The package is provided as a Windows executable Double click on the package to start the installation see Figure 1 1 Filename Metrohm USB Update 1 65 exe 5 Page NOVA External devices tutorial Metrohm AG USB Driver Update Welcome to the InstallShield Wizard for Metrohm USE Update The Installshield Wizard will install Metrohm USB Update on your computer To continue click Next lt Back Cancel Figure 1 The installation of the Metrohm drivers Follow the instructions indicated in the Metrohm AG USB Driver Update installer to complete the installation of the Metrohm device drivers After the drivers are installed Metrohm devices can be connected to the
79. ristaltic pump Metrohm 858 Professional Sample Processor only Figure 41 shows an overview of the Sample processor 858 peristaltic pump command used in a procedure Requires the 6 2141 300 Remote cable 843 Pump Station to Sample Processor 32 Page NOVA External devices tutorial E Sample processor 656 peristaltic pump Sample processor Device name sample processor 0 at lt gt Figure 41 Using the Sample processor 858 peristaltic pump command The Speed parameter can be adjusted between 15 counterclockwise and 15 clockwise in integral steps Setting the value to O will stop the pump 2 6 2 7 Sample processor swing This command changes the position of the swing head on the specified Sample processor tower The position of the swing arm can be defined between O degrees initial position and 90 degrees The 786 Swing head connects to the dedicated connector on the back plane of the Sample processor tower see Figure 42 Swing head connection Figure 42 The Swing head is connected on the back plane of the sample processor tower Figure 43 shows an overview of the Sample processor swing command used in a procedure E Sample processor swing Sample processor Device name sample processor ma Tower _ Angle a Se Figure 43 Using the Sample processor swing command 33 Page NOVA External devices tutorial 2 6 2 8 Sample processor stirrer This command defines the
80. rolled in two different ways e Manually using the dedicated controls e During a NOVA procedure using the provided commands A Warning The Microsoft msxm 6 0 DLL must be installed on the computer in order to run the Sample processor initialize command Please refer to Section 2 3 for more information 22 Page NOVA External devices tutorial A Warning It may be required to manually edit configuration files used by the Metrohm Sample Processor Please refer to the Appendix at the end of this document for more information 2 6 1 Sample processor manual control To access the manual control panel of a connected Sample processor select the device from the Liquid Handling manual control in the view menu see Figure 26 File View Profile Run Tools Help Advanced procedure view 1E 01 es es dl Eg we o Ca gt Setup View Multi Autolab View Measurement View Analysis View User log F11 Autolab display F10 FRA manual control hl pe TT MDE manual control MUX manual control External manual control Liquid Handling manual control sample Processor 856 4589 X Analyte 800 9128 Remote box 770 45892 stirrer 801 2358 Figure 26 Manual control of the Liquid Handling devices is available from the View menu Connected devices are identified as Name Metrohm Device type Serial Number The Autolab display will be displayed with an additional panel for the selected
81. ssage Time limit s 30 se time limit No m lt gt Figure 143 Use the External device receive to wait for a data string from the external device The example shown in Figure 143 can be used to request the temperature of the Julabo HE F34 water bath The answer will be stored in the 0 parameter which can be displayed in a message box as shown in Figure 143 105 Page NOVA External devices tutorial The Device name must be the same as the one defined in the External device initialize command 6 4 External device close This command must always be used to terminate the connection to the external device To terminate a connection add the External device close command to the procedure and define the Device name to terminate the remote control of that device E External device initialize Julabo R5232 Device name Julabo Device type Parag settings f entries E External device clase Julabo Device name Julabo lt gt Figure 144 The External device close command must always be used to release the control of the External device 106 Page NOVA External devices tutorial Appendix Modification of the configuration files for Swing arm control Depending on the type of Robotic arm mounted on the 786 Swing arm specific settings in the configuration files must be adjusted in order to use the Swing arm correctly The Sample processors can be fitted with one or two towers each one c
82. tial 0 V Offset potential 0 V DAC164 1 O0 Autolab LED Driver 0 5 A W Figure 120 The value is defined on the Advanced section of the Autolab control window Click the button to close the window The value specified in the Autolab control window will be displayed in the procedure editor see Figure 121 88 Page NOVA External devices tutorial Control extemal device Autolab LED Driver Value 0 5 lt gt Figure 121 The specified value is updated in the procedure editor 5 TTL triggers DIO The Digital Input Output DIO of the Autolab offers the possibility of synchronizing measurements with external devices that can be controlled by TTL signals Every Autolab instrument is equipped with one or two digital input output connectors DIO that can be used to receive or send a digital TTL trigger Depending on the instrument type two different connector layouts are available 5 1 Autolab PGSTAT series 7 and series 8 and pAutolab II III The Autolab PGSTAT12 128N 30 302 302N 100 and 100N from the 7 and 8 series and the pAutolab type II and III are fitted with two DIO connectors located on the back plane of the instrument Each DIO connector has 25 pins and is divided into three ports see Figure 122 e PortA pins 1 to 8 e Port B pins 17 to 24 e Port C pins 9 to 16 e Pin 25 is the digital ground DGND Section C Section A DGND Section B Section C Figure 122 Overview of the DIO c
83. torial gt Edit Options 0 Automatic Current Ranging Cutoff Autolab control Automatic Integration Time Items Properties t Action triggered when counter Counter Reset counter True Action 1 Options Add Remove Event counter iy OK Cancel Figure 90 Setting the properties to trigger the Event counter every 20 data points Using these settings the counter will track the number of data points and trigger the action when the counter reaches 20 When the action is triggered the counter is reset To define the action to take click the button next to the Action property see Figure 91 68 Page NOVA External devices tutorial amp Edit Options 0 Action tiggered when counter Counter 20 Reset counter True 1 Options Event counter we Figure 91 Specifying the Action 1 2 A new Edit options window will be displayed This window is predefined for a Pulse action however this can be removed see Figure 92 Sy AEE a e o Edit Options Connector Pi Port a A BLES va ts Eat SN 1000 Final edad Remove Figure 92 Edit the Pulse action 69 Page NOVA External devices tutorial The following settings can be defined for the TTL pulse e Connector the DIO connector used to send the trigger P1 P2 e Port the DIO port used to send the trigger A B C e Pulse value the decimal value of the pulse byte state 0 255 e Duration the duration of the pulse in
84. update progress is shown in the Firmware Updater When all the data has been sent to the spectrometer a message will be shown see Figure 97 v AS5216 Firmware Updater lol x File Help Port com Open Fort Information Update Firmware al File Transfer Status Success M Please toggle the Power Supply c q USB cable to the AS55216 Figure 97 A message is shown at the end of the firmware update As indicated by the application remove the USB cable from the computer to power down the spectrometer Wait a few seconds then plug the USB cable in the 74 Page NOVA External devices tutorial computer again to power up the spectrometer again Click the OK button to continue see Figure 97 The firmware is now updated Close the Firmware Updater application The same application can be used to reset the firmware of the spectrometer to the factory default version 4 Analog Control of External devices Several external devices including accessories provided by Metrohm Autolab Autolab RDE Autolab LED Driver can be controlled using an analog voltage in a range of 10 V Similarly some external devices provide readout of a measured signal as an analog voltage in the same voltage range 10 V Autolab instruments are fitted with a Digital to Analog converter DAC which can be used for controlling external devices using a user defined analog voltage between
85. us e Final value the decimal value of the rest byte state of the pulse 0 255 Please refer to Section 5 of this document for more information on TTL triggering The settings specified in Figure 92 will send a 10 ms long pulse to the Avantes spectrometer using Port A of Connector P1 The pulse state will be 255 each pin of the A port is set to High and the rest state will be O each pin of the A port is set to Low Click the button to close the editor and return to the initial Edit options editor Click again to close it and return to the procedure editor 3 3 4 Shutter control Some Avantes light sources are fitted with a remote controlled shutter This shutter is controlled by a TTL level When remote control of the shutter is enabled the shutter will be closed when the level is low 0 V and will be open when the level is high 5 V The shutter position is maintained as long as the TTL value remains unchanged Depending on the type of Autolab used the shutter is controlled through different pins 1 For the PGSTAT101 PGSTAT204 M101 and M204 module the TTL level is sent by pin 8 on the DIO12 connector with respect to the digital ground embedded into the DIO connector A dedicated cable is available for these Autolab instruments preconfigured for the correct digital control of the Avantes light source 2 For all the other Autolab instruments except the PGSTAT302F the TTL level is sent by pin 1 of
86. utolab instruments preconfigured for the correct digital control the Avantes spectrometer 63 Page NOVA External devices tutorial The minimum duration of the TTL pulse depends on the type of Avantes spectrometer Please refer to the AvaSpec user manual for more information Typically 5 ms is enough More information on TTL triggering can be found in Section 5 of this document To specify the TTL pulses to send to the AvaSpec spectrometer during a measurement the Options need to be edited This is only possible for the commands that support the use of Options Click the button in the procedure editor to open the Edit options dialog or click the button from the quick access toolbar see Figure 85 E vat time s Optimize current range 8 CY staircase i COCOCR 00 OOOO start potential v Upper vertex potential w 1 000 Lower vertex potential 6 1 000 stop potential v 0 000 Number of stop crossings 2 step potential v 0 00244 ocan rate v s 0 1000000 Estimated number of points 1650 Interval time s 0 024400 oignal sampler Time WE 1 Potential vwE1 Current ma Options 1 Options m Fotential applied aray Cv Time lt array gt 5 WE Current aray A ocan lt Array WEN Fotential lt aray Cv Index lt array gt ives E aa Set cell Ct x Figure 85 Opening the Edit options in the procedure editor The Edit options window will be displayed
87. utolab control Automatic Integration Time ltems Properties Add Remove Event counter OK Cancel Figure 88 Adding an Event counter to the list of items on the Autolab control tab The Event counter item will be added to the frame on the left hand side of the Autolab control tab The properties of the Event counter will be displayed in the frame on the right hand side see Figure 89 66 Page NOVA External devices tutorial F Edit Options 0 Automatic Current Ranging Cutoff Autolab control Automatic Integration Time Items Properties Action tiggered when counter we Counter 1 Reset counter True Action 1 Options Add Remove Event counter ka OK Cancel Figure 89 The Event counter and the properties of the counter The following properties can be set for the Event counter using the provided editor e Action triggered when counter gt lt defines the condition for the counter to trigger the action defined for the Event counter e Counter defines the counter value to use in the counter test using the condition defined in the properties Reset counter true false defines if the counter needs to be reset when the Event counter is triggered e Action defines the action to perform when the Event counter is triggered For example to send a TTL pulse to the Avantes spectrometer every 20 data points set the properties as shown in Figure 90 67 Page NOVA External devices tu
88. xternal devices tutorial The settings can be selected from a list of predefined settings using the Signal name drop down list or can be defined for a specific device It is possible to save the defined settings by specifying the following parameters e Signal name the name used to identify the external signal e Signal unit the units of the external signal e Conversion slope the conversion of voltage to specified units of the external signal e Conversion offset the offset for the conversion of the voltage to units of the external signal For example for the Autolab LED Driver available as a default from the drop down list the settings are the following see Figure 105 ADC164 1 Signal name W Signal unit A Conversion slope 1 AM Conversion offset 0 A Figure 105 The default settings for the Autolab LED Driver input e Signal name Autolab LED Driver e Signal unit A e Conversion slope 1 A V e Conversion offset 0 A It is possible to define parameters for custom external signals and save them by clicking the 4 button next to the Signal name parameter For example the settings shown in Figure 106 correspond to the current readout from a Kikusui PLZ164WA electronic load ADC 164 1 signal name Kikusul PLATERA nr Signal unit Conversion slope Conversion offset 0 A Figure 106 Defining and saving the settings for a custom signal Kikusui PLZ164WA 80 Page NOVA External devices tutorial O
89. xxx in binary If this Trigger byte is defined in the Wart on DIO trigger command the measurement will be allowed to proceed only when pins 5 6 and 8 are set to high position AND pins 4 and 7 are set to low position The status of pins 1 2 and 3 is irrelevant During the execution of the Wart for DIO trigger command the port status is checked every 100 ms When the Wart for DIO trigger command is encountered the Autolab display shows the status of the port at the bottom of the window see Figure 137 This value is updated every 100 ms Autolab display ea a Autolab manual control U3AUT 0530 voltage current vf LA PSTAT HSTAB status current range Waiting for DIO trigger 11111111 255 Figure 137 The Autolab display shows the status of the DIO port 101 Page NOVA External devices tutorial 6 Recommended Standard 232 RS232 The RS232 standard describes a communication method where information is sent bit by bit on a physical channel The information must be broken up in data words The length of a data word is variable usually between 5 and 8 bits For proper transfer additional bits are added for synchronization and error checking purposes It is important that the transmitter and receiver use the same number of bits Otherwise the data word may be misinterpreted or not recognized at all The current version of NOVA supports the control of external devices through RS232 communication Four dedicated

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