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Inspira 557058 9

1. Turn off power and remove power cord from power module Use a straight blade screw driver to pry open the access door Remove the fuse holder and then remove the fuses from this holder as shown in figure 8 below Replace fuses using only 1 amp 250 volts TypeT fuses Then replace the fuse holder Note A fuse which is not operator replaceable is located on the power supply This fuse is rated 5 amps 250 volts Cc Lo O Ug Figure 8 Fuse Replacement Operation Getting Started 13 7 ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual HARVARD O OO SE pl VO O C CO 2 cx 90 Sooo Remote Indication Tidal Volume Ventilation Mode bin pau Vt aree m Wl RATE xxxBPM gt gt gt gt nnd TES Respiration Rate Run Stop Figure 9 Main Operating Screen Volume Control Mode Turning Ventilator On Power must be properly supplied to the ventilator according to the power specifica tions Locate the power switch on the rear of the ventilator Toggle the switch so that the l side of the switch is pressed down The ventilator is now powered on Volume Controlled Ventilation With machine powered ON press and release the SELECT MODE key until VOL mode is selected Press SET and WEIGHT and enter the animal s weight in kg Press and hold parameter keys in order t
2. 3 0000 BEE Selects sigh volume 4 Enter Sigh Freq Si ging Breaths Enters sigh volume returns prompt for sigh frequency 5 OO Enter Sigh Freq 1 Sigh 12 4Breaths Selects sigh frequency 61 C2 Enters choice and returns to main screen Entering Sigh Breath Pressure Control Mode A sigh pressure and sigh frequency must be set in order to activate the sigh breath function in pressure control mode Disabling Sigh Breath To disable sigh breaths enter Sigh Volume Tidal Volume volume control mode or set Sigh Frequency to 1 sigh 0 breaths SIGH BREATHS ARE AUTOMATICALLY DIS ABLED WHENEVER A NEW ANIMAL WEIGHT IS ENTERED Operation Respiration Profiles 24 PUSS 7 n uw x_ _ NW__OO _ O Sigh Pressure Pressure Control Mode Only Step Key Display 1 Press Parameter Key Enter Sigh Pressure 2 tuin Enters sigh configuration mode 3 A Enter Sigh Pressure 35 cmH20 Selects sigh pressure 4 Ce Enter Sigh Prec Sigh _ Breaths Enters sigh pressure returns prompt for sigh frequency 5 2 Ener Sigh Pes Som 52 Breaths Selects sigh frequency Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Operation Respiration Profiles SZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Entering I E Ratio e Thel E ratio is the ratio of the time needed for inhalation compared to time needed for exhalation Th
3. xxxx Biles 1 8 8 Displays current RS 232 setting Operation Getting Started DO I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Warnings and Faults Pump Stall Pump Stall Alarm Stop This fault indicates that the expected motion of the piston axis does not match the actual motion This is a critical fault and requires operator intervention The valve is set to the expiration position and the machine is commanded to STOP The user is always notified via a visual alarm and also by an audible alarm if enabled Valve Stall Valve Stall Alarm Stop This fault indicates that the expected motion of the valve axis does not match the actu al motion This is a critical fault and requires operator intervention The machine is commanded to STOP The user is notified via a visual alarm and audible alarm if enabled Motor Drive Fault Motor Drive Fault Stop This fault indicates a problem with the step motor drive This is a critical fault and requires operator intervention The machine is commanded to a STOP state Airway Over Pressure Overpressure Alarm This fault indicates that an overpressure condition has been detected in the airway The user is always notified via a visual alarm and also by an audible alarm if enabled An overpressure in Volume Control mode is defined as a pressure greater than the aver age measured peak pressure averaged over 10 samples not counting sigh breaths plus 1
4. use a 9 pin male to 25 pin female shielded cable 5154 527 Note Do not use a Null Modem type cable RS232 Cable Connection Usecorrect cable The recommended cable length is 3 meters maximum Turn power off to Inspira and to the external device before connecting the cable into the connectors e Check that the Inspira signal ground pin 5 is connected to the external device signal ground e Use shielded cabling to connect Inspira s chassis ground to the chassis ground of the external device Serial Communication Setup To configure Inspira for serial communications press Set key followed by the RS232 key The display should read RS232 Setup Enter to Continue Press Enter key to display the RS232 input output selection menu The display should now read Press 1 Input port 2 Output port Select the port you would like to configure Input is used for serial connection to a pc Output is used for a daisy chain setup The display should now show the RS232 port setup menu which reads Press 1 Baud 2 Bits 3 Parity 4 Addr Step Key Display 1 Press Parameter Key TUP 2 CONTI NUE Enters RS232 configuration 3 PRESS i Enters port selection menu 4 O PRESS 1 BAUD 2 BITS 3 PARITY 4 ADDR NPUT PORT TPUT PORT c Selects input port and enters port configuration menu or 2 PRESS 1 BAUD 2 BI TS 3 PARITY 4 ADDR Selects output port and enters port configuration menu O
5. G Recommended Cylinder Size eenen eeeeneee 53 H Recommended Port Size 54 I Using Inspira with Pressurized and Anesthetic Gasses 55 J Generating PEEP with Inspira ASVv 56 Warranty and Repair Information JJ LLL Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All ventilators are calibrated at rated voltage and frequency Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials This warranty does not extend to dam age resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within th
6. PREVENT HAZARD OR INJ URY Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Any signal wire connec tions to the Inspira unit must be no longer than 3 meters Observe all Terminal Ratings Review the operating manual to learn the ratings on all Inspira connections Do Not Operate Without Cover Make sure that the top cover is securely in place before connecting to power source to avoid injury to body and damage to the product Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Man
7. Remove foam packing inserts from both ends of the ventilator Locate and carefully remove air cylinders and power cord from shipping material Visually inspect both the ventilator and the air cylinders for any damage that might have occurred in the shipping process The unit is shipped without the air cylinder installed Please refer to Air Cylinder Installation to properly install the cylinder see pages 11 to 12 The Inspira is shipped from the factory with a North American line cord with a U S three wire molded power plug on one end and an IEC320 C13 connec tor on the other Use only an approved AC line cord with a molded 1EC320 C13 connector certified for country of use Only connect to ground ed power receptacles to help ensure proper grounding do not use adapter plugs Read the manual to become familiar with all features and functions of the Inspira Location Requirements A sturdy level clean nonflammable and dry surface Minimum of 1 inch 2 5 cm clearance around the pump Adequate power supply Room temperature 4 to 40 C 40 to 104 F Relative humidity of 20 to 80 A well ventilated room WARNING Do not use in the presence of explo sive gasses or in a 100 oxygen environment Typical Operation The Inspira ventilator is designed to respirate small animals with a body mass ranging from 15 grams to 10 kilograms It can operate at respiration rates of 5 to 200 breaths per minute bpm and can deliver tidal vo
8. Tidal Volume in cc Rate Respiration Rate in BPM R Remote Mode Indication Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Operation Getting Started 15 am a Vt Xxx xxcc R VOL RATE xxxBPM gt gt gt gt CC Rate eS wei C Sigh peo Pressure em V Set Keys Toggle Keys Data Entry Keys Figure 10 Front Panel Set Keys Toggle Keys Set Pressing these keys toggle through the Allows modification of a data item To modify a data item press the relevant key after pressing the Set key Also used to toggle different options when prompted Rate Displays Sets current respiration rate Volume Displays Sets current tidal volume in vol ume mode Weight Displays Sets current animal weight Sigh Displays Sets sigh parameters and manu ally triggers a sigh breath EE Ratio Displays Sets the current inspiration to expiration I E ratio Pressure Displays Sets pressure limits RS232 Displays Sets current RS232 device s attached Successive states of the key s function Run Stop Starts Stops the ventilator When stop ping the ventilator will finish its current stroke before ceasing ventilation Select Mode Toggles through available run modes with each press of the key It is also used to select entry choices in hardware setup Data Entry Keys 1 2 3 4 5 6 7 8 9 0 Used to enter numeric data values or access sp
9. by placing the plunger portion of the air cylinder on the end of Inspira s white plunger Carefully slide the air cylinder back on the white plunger compressing the air cylinder just enough to allow the front end of the cylinder to be lowered into place Once the air cylinder is fit in place screw the air cylinder s threaded nipple into the valve block and hand tighten the cylinder in place CAUTION Do not overtighten cylinder Overtightening may cause glass cylinder to crack Now that the air cylinder is in place rotate the lead screw into position so that the flag pin in between both the front and rear switches To complete the installation replace the top cover of the Inspira unit When Inspira is powered on it should display the correct cylinder size after initializing The ventilator automatically detects which size cylinder is installed Large Air Cylinder Small Air Cylinder Front Switch Rear Switch White Flag Pin Plunger Lead Screw Coupling Figure 12 Changing Air Cylinders 37 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Operation Advanced Hardware Setup Inspira s microprocessor based system provides hardware setup options and diagnos tic tests to be run on the ventilator Hardware setup menus allow the user to config ure specific hardware in the ventilator while the diagnostics provide some tests to help in troubleshooting faults These options are found in
10. due to one or more reasons High PEEP Alarm Inspira ASVp Only High PEEP Alarm If the actual end expiratory pressure EEP exceeds the desired PEEP plus 5cmH20 or 11cmH20 whichever is less a High PEEP Alarm is reported This alarm is self clearing on removal of the fault condition Operation Respiration Profiles PO ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Entering Respiration Rate To enter acustom respiration rate press the Set key followed by the Rate key The dis play should now read Enter Resp Rate Enter the rate in breaths per minute BPM using the numeric keypad Only whole numbers will be accepted as valid rates Press the Enter key to enter the selected rate If the selected rate is not within the Safe Range a prompt will ask if you would like to override the Safe Range value see page 32 Step Key Display 1 Press Parameter Key Enter Resp Rate 2 aan Enters respiration rate configuration mode S1 DE a 123 Selects rate in breaths per minute Enters choice and returns to main screen Entering Tidal Volume Volume control Mode Only The inspiration and expiration volumes are always the same To enter a custom inspiration expiration volume press the Set key followed by the Volume key The display should now read Enter Tidal Vol Use the numeric keypad to enter the inspiration expiration volume Press the Enter key to enter the selected volume If the s
11. mammals in a resting state and do not account for additional dead space which may be introduced by the user s system set up Dead space volume should be calculated and the tidal volume should be increased accord ingly The animal s respiratory health must also be considered when adjusting respi ratory parameters Appendix F 9 700 ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual 4 How do create a PEEP pressure with the Inspira ASVv To create a PEEP pressure when using the Inspira ASVv simply connect a length of tubing to the Exhaust port and submerge the other end in a column of water The depth of the end of the tubing in cm below the water surface will give the PEEP pressure in cmH20 See Appendix J 5 How do I connect the Inspira to pressurized gas sources When connecting any volume controlled piston cylinder type ventilator to a pres surized gas source that gas must be equalized with atmospheric pressure before being drawn into the ventilator If itis not unknown and possibly lethal tidal volumes will be delivered to the animal A set up as shown in Appendix may be used for this purpose 6 How do I use anesthetic gases with the Inspira Refer to Appendix I for setting up the Inspira with a vaporizer and pressurized gasses When using anesthetic gasses keep in mind that total system volume will impact delay times in getting a change in gas mixtures to the animal Vent or scrub expired gass
12. pressure setting only available in pressure control mode Operation Respiration Profiles 29 Fo TTT Assist Pressure Step Key Display 1 Press Parameter Key 2 PRESS T Enters assist pressure configuration mode 3 2 Enter Asst Presale D cm H20 Enters assist pressure setup 4 G Enter Asst Pressiures 5 cmH20 Selects assist pressure 5 Enters assist pressure and returns to main screen INSP 2 ASST PEEP Was Chia Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Note 1 Sigh pressure setting only available in pressure control mode PEEP Pressure Step Key Display 1 Press Parameter Key 2 EEE Enters peep pressure configuration mode 3 O Enter PEEP Pressure 9 _ cm H20 Enters peep pressure setup 4 Enter PEEP Pressure 5 cmH20 Selects peep pressure 5 Enters peep pressure and returns to main screen Note 1 Sigh pressure setting only available in pressure control mode 30 Operation Respiration Profiles Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Sigh Pressure Pressure Control Mode Only Step Key di Ce 2 Enters weight configuration mode 3 O Selects weight S XA Enters weight Moves cursor to select yes or no Selects choice and returns to main screen ZI Starts and stops ventilation Display Press Parameter Key Enter SIGH Pressure _ cmH20 Enter SIGH Pressure 35 cmH20 E
13. sound the alarm When finished press the Enter key to return to the diagnostics menu Step Key Display 6 Enters alarm test mode and sounds alarm LE Exits alarm test mode and returns to main screen Fault Log To read fault log information enter the diagnostics menu and press 4 to advance to the next menu of tests Press 1 to momentarily display fault code information and an error count The fault code is a hexadecimal code indicating faults and warnings detected since the fault log was last cleared The error count tracks the number of events which have occurred and caused the machine to reinitialize The machine will reinitialize automat ically three times before requiring user intervention Events such as valve stalls and end oftravel faults trigger the automatic re initialization in an attempt to restore the machine to normal operation If the fault occurs more than three times a more serious problem may exist and the machine will no longer automatically reinitialize In this case the user should contact the manufacturer for assistance Power cycling clears the fault and error count Fault log information is retained until the fault log is cleared Step Key Display 6 LOG 2 CLR FLT S 4 NEXT 5 BACK Advances to next menu of diagnostic tests Fault Log 0x0000 Error Count 0 Displays Fault Log and Error Count Returns to previous screen after a few seconds Note See previous pages for steps 1 t
14. the hardware settings menu which is accessed by pressing the Set key followed by the Enter key Press Enter again to enter the hardware configuration menu n e 0 Ke Key Display set Press Parameter Key HARDWARE SETUP ENTER TO CONTINUE EH 2 Enters hardware setup 3 Displays hardware setup menu PRESS 1 ALARM 2 1 0 3 DISPLAY 4 NEXT Audible Alarm On Off To toggle the audible alarm on or off enter the hardware configuration menu Press 1 to enter the alarm setup The display should read Press Select Mode to Toggle Alarm and display whether the alarm is currently on or off Pressing the Select Mode key toggles the alarm on or off enters the selection and then returns to the main screen WHEN YOU TURN OFFTHEAUDIBLE ALARM IT IS OFF FORALL FAULT FUNCTIONS Step Key Display 4 O PRESS SELECT MODE TO TOGGLE ALARM On Enters alarm configuration Select PRESS SELECT MODE TO 5 TOGGLE ALARM Off Toggles alarm setting 61 C Accepts value and returns to main screen Note See above for steps 1 through 3 Operation Advanced FB ML Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual 1 0 Setup To review or modify the I O settings enter the hardware configuration menu Press 2 to enter the I O setup The display should now read Set I O Press 1 Digital 2 Analog Pressing 1 selects Digital I O configuration This feature is a future option and is not presen
15. yes or no 6 Selects choice and returns to main screen 7 Starts and stops ventilation Operation Getting Started Ir ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Starting and Stopping Ventilation To start and stop ventilation simply press the Run Stop key When stopping Inspira will finish its current stroke before stopping completely The valve will always be in the expiration position when a STOP has been commanded Displaying Ventilator Parameters Inspira allows you to easily check the current ventilation parameters by using one key commands To display a specific parameter simply press and hold the appropriate key Key Display m Resp Rate xxxBPM Displays current respiration rate oume Tical Moll tor CE Sigh Vol RUN S XX Displays current tidal volume and sigh volume volume control mode C Tidal Wohl se cec Displays current tidal volume pressure control mode io Weight xx xxxkg Displays current weight setting sm Sigh Vol xxx xxcc Sigh Freq 1 xxx Displays sigh breath settings C Sigh Pres xx cmH20 Si gin Fre Wi sso Displays sigh breath settings pressure control mode ena I nspiration x x Displays I E ratio settings oe Insp P Limit xxcmH20 Assist P xxcmH20 Displays current pressure settings oe Paw xx EEP xx Asst x Pil mes BEEP sx ce adl Displays current pressure settings ASVp rs Baud
16. 0 cmH50 or 67cmH50 whichever is smaller This limit is adjusted during sigh breaths to 150 of the average value or 67 cmH350 whichever is smaller The option al user entered inspiration pressure limit is used as a secondary pressure check The default limit is 35 cmH 0 If the average pressure should rise up and exceed this value the user is notified via a visual alarm and also by an audible alarm if enabled Airway overpressure conditions cause the inspiration stroke to stop the machine immediately cycles to expiration After completing expiration the machine will con tinue to run the current respiration profile repeating the above steps if the overpres sure condition continues If the fault is cleared the alarms visual and audible will self clear Otherwise the alarms will continue requesting operator intervention Operation Getting Started AT ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Occlusion Alarm Inspira ASVp Only Occlusion Alarm If the peak pressure limit is reached within the first 40 of the inspiration stroke an occlusion alarm is reported This indicates that a restriction may be present in the air way or that the airway resistance may be too high for the flow rate Occlusions sys tem restrictions due small tubing and or port diameters or incorrect respiratory parameters could be the cause This alarm is self clearing on removal of the fault con dition Assist Alarm Assist Ala
17. 0cc Cylinder 100cc Cylinder 77 10cc Cylinder 54 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Appendix H Tidal Volume cc Recommended Port Size vs Tidal Volume amp Rate 5 re gm d 20 25 50 75 100 125 150 175 200 Rate BPM Max Tidal Volume cc Large Ports Min Tidal Volume cc Large Ports Max Tidal Volume cc Med Ports Min Tidal Volume cc Med Ports Max Tidal Volume cc Small Ports Min Tidal Volume cc Small Ports Large Ports NW Medium Ports IN Small Ports 55 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Appendix I Using Inspira with Pressurized and or Anesthetic Gasses Flow EVI Regulators From Animal N A A Pressure O L Lr Regulator source O O e Vapor Gas 1 To Exhaust Side o Aniamal stream _ Liquid Anesthetic x Vaporizer Breathing Bag Gasn or air Optional Bleed LIT w To Scrubber Tracheal Cannula Or Vent Hood or Endotracheal Tube WARNING DO NOT VENT ANESTHETIC GASSES TO ROOM AIR Notes 1 Set flow rate into vaporizer per manufacturer s specification 2 Flow into breathing bag should be equal to or slightly g
18. Harvard Inspira Advanced Safety Ventilator User s Manual Inspira Advanced Safety Ventilator Volume Controlled 55 7058 Inspira Advanced Safety Ventilator Pressure Controlled 55 7059 Table of Contents 1 ne Warranty and Repair Information eere 3 General Safety Summary creer enr reinen entrants 4 Introduction 5 D Theory of Operation ii 5 FEALUresi iu eee ie 5 8 ELE E 7 12 y Initial Setup and Location Requirements sssss 7 Typical ODeFallOns secte tei are tte titii entendi 7 Ventilator Connections c Airway Tubing Connections Front Panel 8 Electrical Connections Front Panel 9 m Electrical Connections Rear Panel 10 Air Cylinder Installation 11 Fuse Replacement i 12 Op ration C va savisuti sanava Sa sT ES aa 13 44 E Getting Started Turning Ventilator On i 13 Main Operating Screens i 14 Key FUNCHONS T a 15 Safe Range Respiratory Profiles sss 16 Entering Animal Weight see 18 Starting and Stopping Ventilation 19 Displaying Ventilation Parameters 19 Warnings and Fa lts 2 teni tette 20 Respiration Profiles Entering Respiration Rate sseess 22 E
19. This drop initiates a ventilation cycle The sensitivity of the Assist Mode is adjustable from 1 to 10 cmH50 The ventilator lets the animal initiate breaths independently as long as its breathing rate is greater than the respiration rate set by the user or calculated by the microprocessor If the animal does not try to initiate a breath Inspira warns the user Airway Pressure Monitoring The ventilator monitors the pressure of gas delivered to the animal being ventilated This safety feature prevents the operator from over or under pressurizing the lungs of the animal When Inspira detects an over pressurization condition it sounds an alarm stops the inspiration stroke and then cycles to expiration Inspira continues to ventilate but does not deliver enough gas to over pressurize the lungs Overpressure conditions may result from occluded or restricted airways When Inspira detects an under pressurization condition it sounds an alarm This condition could result from an airway disconnect or large leaks Once again Inspira continues to ventilate while requesting user intervention These features ensure that the animal is kept safe from improper ventilator setup PEEP Inspira ASVp Only Positive end expiratory pressure PEEP is settable in the InspiraASVp in both volume and pressure controlled modes The PEEP is adjustable from 0 to 10 cm H70 Installation l fp Initial Setup 1 2 3 5 Remove unit from box
20. Y 3 E S 4 NEXT 5 BACK Returns to previous menu FIL PR Exiting the Diagnostics Pressing the Enter key while in the diagnostic menus will exit and return to the main operating screen Operation Advanced M ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Power Failure The Inspira ventilator may be connected to an uninterruptable power source UPS such as a battery backup to protect against power failures Choose a UPS that has an output power capacity sufficient to power a 75 watt load for the desired length of time to protect A UPS rated at 650 VA will typically provide more than 1 hour of operation on a fully charged battery If a UPS is not used the machine will stop ventilating for the duration of the power failure When the power is restored the machine will power up in its last commanded state If that state was RUN it will continue ventilation with the last entered respiratory parameters Harvard Apparatus recommends use of a UPS device or other backup power source when performing unmonitored ventilation Note There are many manufacturers of UPS devices Some examples are American Power Conversion www apc com and Belkin www belkin com Contact the UPS manufacturer for help in choosing the right sized unit General Maintenance and Cleaning To ensure that your Inspira operates correctly only use the ventilator according to the guidelines provided in this operating manual If the ventilator
21. ach inspi ration stroke This signal is available at the front panel through a BNC connector The pulse width equals 20 ms 10 ms This signal can be used in conjunction with other devices which need to operate in sync with the ventilator s respiration stroke The falling edge must be used for sync timing Sync Out Inspira ASV The sync signal is a logic high for the duration of the inspiration time and a logic low during the expiration time The rising edge corresponds to the start of the inspiration cycle and the falling edge corresponds to the start of expiration Analog Out The analog out ANALOG OUT 1 is used to measure the ventilator s airway pressure This output can be monitored using a data acquisition system or by an oscilloscope To view or record this signal connect the data acquisition system or oscilloscope to the analog out BNC connector on Inspira s front panel Operation Advanced iL Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Remote Control Monitoring Serial Communication The Inspira is equipped for serial communication through two RS232 ports Inspira uses a Harvard Apparatus Command Line protocol as the basis of its serial commands These serial commands can be found in Appendix C RS232 Cable Selection To connect PC At or laptop computers you will need a 9 pin male to 9 pin female shielded cable Harvard Apparatus part number 5154 353 For PCs with a 25 pin seri al port connection
22. aveforms A Patienttriggered breath B Machinetriggered breath due to no detected patient effort A B assist window time sec sigh breath 3 4 time sec Figure 13 Sample Waveforms Appendix F 51 NEN LLL Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Frequently Asked Questions 1 When I start ventilation why is underpressure constantly displayed If no connection is made to the To Animal port this is the proper indication The fault will clear when airway pressure is detected If proper connections have been made there is most likely a leak in the system Make sure that the O ring has been installed properly before installing the glass cylinder Verify that the cylinder has been tightened properly If barbed ports at the front of the machine have been replaced make sure that they are fully tightened to be flush with the front bezel Finally check for leaks in the tubing and at the animal s airw ay If you are using the Inspira ASVp in pressurecontrol mode and the average airway pressure is greater than 3 cmH20 the machine will attempt to adjust the flow rate to increase the airw ay pressure If an underpressure condition continues Paw much less than desired and the tidal volume is larger than expected check for system leaks 2 When I change or install a cylinder why is it not detected properly The Inspira uses two micro switches to detect the two
23. d when the device detects a slight vacuum in the airway Expiration The process of breathing out exhaling a gas or gasses Flow Rate The rate of gas flow expressed in L min or mL min EE Ratio The ratio of inspiration to expiration times As used in this context however it is expressed as the percent of inspiration time to total respiration period An I E Ratio of 1 1 is equivalent to 50 inspiration time An I E Ratio of 1 4 is equivalent to 20 inspi ration time Inspiration The process of breathing in inhaling a gas or gases Inspiration Pressure The pressure to which the subject s lungs are inflated during inspiration PEEP Positive End Expiratory Pressure Residual Volume The smallest volume to which the subject s lungs can slowly be deflated Respiration Rate Breathing rate expressed in breaths per minute BPM Sigh A deeper than usual breath which expands the lungs to a greater capacity and redis tributes the lung surfactant that tends to puddle in the alveoli during normal respira tion Sigh Volume The inspiration volume during a sigh breath some percentage greater than normal tidal volume for the subject Sigh Pressure The inspiration pressure during a sigh breath some percentage greater than normal inspiration pressure for the subject Appendix E SO ooo pressure D Sg x E e 5 FA n o a 10 PEEP assist trigger threshold 0 volume cc Sample W
24. different cylinder sizes The rear most switch actuates when the 10cc cylinder is installed Both switches actuate when the 100cc cylinder is installed If the 10cc cylinder is not being detected first make sure that the rear most actuator arm is resting against the side of the cylinder If it isn t just reposition the actuator arm Next make sure that the switches are actu ating Gently pull back on the actuator arm s contacting the installed cylinder and listen for a click If the switch clicks it had not been actuated This indicates that the actuator arm s bend angle has not been set properly This may be easily corrected by gently increasing the bend angle of the actuator arm s 3 Why does it seem like the volume delivered is too low It is possible that there is a system leak Check for leaks as described in the answer to question 1 In volume control mode if the inspiration pressure limit is reached the machine will cycle to expiration before total tidal volume is delivered Make sure that restrictions in the airway circuit are minimized and make sure that the pressure limit isn t set too low If you are using side stream gas monitoring tidal volume will need to be adjusted increased to account for the sample volume If system dead space is high tidal volume will need to be adjusted to account for it Please note that the machine s calculations for tidal volume and respiration rate based on the animal s weight are for healthy
25. does not work proper ly contact Harvard Apparatus for appropriate instructions To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the clear display window For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alcohol Appendix A 45 General Specifications Specifications Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Animal Size Volume Port Sizes Control Modes Breathing Rate Resolution Inspiratory Time I E Ratio Inspiratory Flow Gas Supply Inspiration Pressure Full Range Safe Range Accuracy Resolution Sigh Frequency Sigh Pressure Full Range Safe Range Respiratory Assist Assist Trigger Signal Inputs External Trigger Input Signal Outputs Sync Out ASV Sync Out ASVp AnalogOuti Airway Pressure Signal Pressure Range Sensitivity Signal Level Offset Response AnalogOut2 Remote Communications Display Power Dimensions H x W x D Weight 15 g to 10 kg 0 1 to 100 ml 1 8 27 NPT threaded nylon barbed ports accommodates 1 16 1 8 3 16 ID Tubing Default 1 8 ID Tubing Volume or Assist 5 to 200 bpm 1 breath min 20 to 80 of cycle time Default 50 4 1 to 1 4 Default 1 1 0to 12 SLPM Room air or nonflammable mixed gas 5 to 50 cmH20 15 to 25 cmH20 4 1 cmH20 1 cmH20 lin 5 to 1 in 250 breath
26. e one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or drawing We offer a complete reconditioning service CAUTION This apparatus is not registered with the FDA and is not for clinical use on human patients A CAUTION Not for clinical use on human patients General Safety Summary 4 I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Please read the following safety precautions to ensure proper use of your Inspira ven tilator To avoid potential hazards and product damage use this product only as instructed in this manual TO
27. e volume of the inhalation breath is always the same as the volume of the exhalation breath There are no predetermined safe range values for E ratios The default I E ratio is 50 To enter a different E ratio press the SET key followed by the I E RATIO key The dis play should now read Enter Inspiration 20 80 Use the numeric keypad to enter the whole number percentage of inspiration which must be between 20 and 80 The percent of expiration is determined from the value for inspiration Note Due to electro mechanical system limitations Inspira might not accept some I E Ratio values for certain respiration profiles If a desired I E ratio is not accepted when using the small cylinder try switching to the larger cylinder This may allow acceptance of the desired ratio Step Key Display 1 Press Parameter Key 2 Enter Inspiration 20 80 Enters I E ratio configuration mode 3 OG Enter lnspiration 2095 809 40 Selects inspiration ratio 4 Enters inspiration ratio and returns to main screen Operation Respiration Profiles PO I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Entering Pressure Settings Inspira ASVy Inspiration Pressure The Inspiration pressure is a pressure threshold which is used to detect airway over pressure conditions If the average airway pressure rises above this pressure thresh old an alarm is sounded if enabled and the display sho
28. ecial features C Clear clears numeric data values prior to entry Enter Saves and stores displayed data value in memory when setting a data item Operation Getting Started 169 7 ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Safe Range Respiratory Profiles Setting up theASV ventilator is both easy and safe thanks to the Safe Range software Once the animal s body weight has been entered the software computes the median settings for tidal volume and respiratory rate These same equations are used to cal culate a Safe Range around the median values Inspira uses the entered weight to calculate the correct tidal volume and respiratory rate for the animal The tidal vol ume in liters is determined by the equation 0 0062 x M where Mp is the animal mass in kilograms The respiratory rate in min is determined by the equation 53 5 x Mp0 The Safe Range is established by computing a range of acceptable values which are less than or equal to 10 of the calculated tidal volume and respiratory rate All the default parameter settings can be overridden If you enter a setting outside of the Safe Range for the body mass of the animal to be ventilated you are warned that the value is out side the Safe Range and asked to confirm the setting SAFE RANGE Pressure Control Mode Inspiration Pressure Range Safe Range 15 to 25 cmH20 Full Range 5 to 50 cmH20 Sigh Pressure Range Safe Range Insp Pres
29. elected volume is not within the Safe Range a prompt will ask if you would like to override the Safe Range value see page 32 Step Key Display 7 set Press Parameter Key 2 us Tidal Vol cc Enters volume configuration mode 3 0009 ae Tidal Vol fee Selects volume 4 C2 Enters choice and returns to main screen Operation Respiration Profiles 8 00000 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Entering Sigh Breath Volume control Mode Asigh tidal volume STv and a sigh frequency must be set in order to acti vate the sigh breath function in volume control mode To enter a sigh tidal volume and a sigh frequency press the Set key followed by the Sigh key The display will now read Enter Sigh Volume and will also show the cur rent STv Use the numeric keypad to enter the desired STv Press Enter to enter selected STv Inspira will now ask for the desired sigh frequency The display should read Enter Sigh Freq 1sigh breaths Again use the numeric keypad to enter the ratio of sigh breaths to regular breaths Note Inspira will only accept whole numbers for this ratio Press the Enter key to enter the selected ratio If the selected values are out of the Safe Range a prompt will ask you if you would like to override the Safe Range value see page 32 Step Key Display 7 set Press Parameter Key 2 Enter Sigh Volume STU _ Enters sigh configuration mode
30. es do not vent to room air WARNING Do not vent anesthetic gasses to room air 7 How do use the Inspira with MRI equipment Since the Inspira has a ferrous metal chassis and components it will be attracted to the MRI s magnetic field Also the machine will create its own electromagnetic fields which could impact measurements made with the MRI It is therefore highly recom mended that the machine be located far from the MRI equipment more than ten feet If the machine is located in the same room as the MRI system tying the unit down to the floor or other immovable object may be required Long lengths of tub ing will be required to connect with the animal s airw ay so total system volume com pliance and flow restrictions must be considered when choosing the tubing diame ter wall thickness and adapters If anesthetic gasses are to be used delay times in get ting an adjusted gas mixture to the animal will be significant and should be deter mined 53 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Appendix G Recommended Cylinder Size vs Tidal Volume amp Rate at 50 I E Tidal Volume cc 5 20 25 50 75 100 125 150 175 200 Rate BPM e Max Tidal Volume cc 10cc Cylinder e Min Tidal Volume cc 10cc Cylinder amp Max Tidal Volume cc 100cc Cylinder g Min Tidal Volume cc 10
31. ests Press 1 to perform the pump test The display should read Pump Test Once the test has completed suc cessfully the display will read Piston Test Success and return to the diagnostic menu Step Key Display 1 PUMP 2 VALVE 7 9 3 NVRAM 4 BACK 8 Performs Pump Test 9 Repeats test Valve Diagnostics To check the respiration valve s functionality enter the diagnostics menu Press 4 and press 4 again to advance to the next menu of tests Press 2 to perform the valve test The display should read Valve Test Once the test has completed suc cessfully the display will read ValveTest Success and return to the diagnostic menu Step Key Display 1 PUMP 2 VALVE UE 8 Performs Valve Test 9 Repeats test Operation Advanced BZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Non Volatile Memory Diagnostics To check the non volatile memory NVRAM for errors enter the diagnostics menu Press 4 and press 4 again to advance to the next menu of tests Press 3 to per form the memory test The display should read No errors yet When done the dis play should read NVRAM test done and return to the diagnostic menu Step Key Display 1 PUMP 2 VALVE a 8 G No errors yet Performs NVRAM test NVRAM Test Done Returning to Previous Diagnostic Menu Testing is complete Step Key Display 1 PUMP 2 VALVE 3 NVRAM 4 BACK 8 1 T LOG ASCER FL
32. g as a safety feature The Inspira ASVp pressure controlled ventilator adds pressure control capability to the ASVv volume controlled platform Inspiration pressure is limited to the user entered pressure setting Flow rates are automatically adjusted by the microcon troller by changing the tidal volume while keeping the inspiration time constant The flow rate is adjusted so that the inspiration pressure limit is reached near the end of the piston stroke ensuring that animals with a higher airway resistance receive the majority of the expected tidal volume and that the ventilator does not prematurely terminate the piston stroke No manual adjustment of inspiratory flow rates is required TheASVp also adds PEEP capability for both the volume and pressure control modes The machine allows a PEEP setting up to 10cmH20 Features Volume Control Mode The ventilator delivers a known volume of gas to the patient on each inspiration stroke Respiration frequency and I E Ratio determine amount of time for inspiration and expiration phases Introduction C Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Pressure Control Mode Inspira ASVp Only The ventilator delivers gas to the patient until the user selected pressure limit is reached Flow rate is automatically adjusted by the machine so that the pressure limit is reached near the end of the inspiration time Respiration frequency and I E Ratio determine the amount of ti
33. haft Turn the lead screw until the flag Installation D ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual pin on the rear of the plunger is vertically indine with the rear switch as illustrated Note When installing the air cylinder do not change the bends of the cylinder detec tion switches or else the correct cylinder will not be detected Now insert the cylin der by placing the plunger portion of the air cylinder on the end of Inspira s white plunger Carefully slide the air cylinder back on the white plunger compressing the air cylinder just enough to allow the front end of the cylinder to be lowered into place Once the air cylinder is fit in place screw the air cylinder s threaded nipple into the valve block and hand tighten the cylinder in place CAUTION Do not overtighten the cylinder Overtightening the cylinder can cause the glass cylinder to crack Now that the air cylinder is in place rotate the lead screw to position the flag pin in between both the front and rear switches To complete the installation replace the top cover of the Inspira unit When Inspira is powered on it should display the correct cylinder size after initializing Fuse Replacement Key Information 1 Make sure the power cord is disconnected from the main supply before serv icing the fuse 2 Use only Type 3AG 1 4 x 11 4 1 amp 250 volt TypeT time delay fuses Harvard Apparatus part number 5113 033 or equivalent
34. hrough 5 Operation Advanced Mp I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Clearing Fault Log To clear the information in the fault log press 2 The fault log will be cleared to 0x0000 but the error count will be retained Step Key Display 6 PT LOG ASCER PET PRES 4 NEXT 5 BACK Advances to next menu of diagnostic tests 7 2 Fault Log 0x0000 Error Count 0 Clears Fault Log and returns to previous screen after a few seconds il 3 Pressure Test Future Feature A pressure test for testing the internal pressure transducer is planned for future ver sions It is not yet implemented Step Key Display 6 UT LOG Zectk Er F PRES 4 NEXT 5 BACK Advances to next menu of diagnostic tests 71 Returns to previous screen after a few seconds 1 3 Advancing Returning to Other Diagnostic Tests Pressing 4 will advance to the next menu of tests Pressing 5 will return to the pre vious menu of tests Step Key Display 6 T LOG ASCER PLI ES 4 NEXT 5 BACK 1 PUMP 2 VALVE A Advances to next menu of diagnostic tests OR 1 DSPLY 2 KEY 3 ALARM 4 NEXT Returns to previous menu of diagnostic tests Operation Advanced Ap I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Pump Diagnostics To check the pumping mechanism functionality enter the diagnostics menu Press 4 and press 4 again to advance to the next menu of t
35. in Lead Screw Coupling Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Figure 7 Air Oylinder Installation Air Cylinder Installation Key Information 1 Use ONLY Harvard Apparatus approved air cylinders Any other non approved cylinders may damage the Inspira unit 2 Do not oil or grease the air cylinders Inspira s special air cylinders are self lubricating requiring no additional lubrication Applying oil or grease may damage the air cylinders 3 When switching the air cylinders the power must be SWITCHED OFF 4 Tampering with any components besides the air cylinders inside of the Inspira will void the warranty CAUTION Electrostatic discharge ESD can damage compo A nents on the circuit boards Avoid contact with the circuit boards when changing cylinders Procedure Before the Inspira can be used for the first time one of the two provided air cylinders 10 cc and 100 cc must be installed Choose the proper cylinder depending on the size of the animal to be ventilated Remove the top cover of the ventilator by remov ing the seven phillips head screws which hold the top cover in place Once the top cover is removed locate the mounting area for the air cylinders as seen in figure 7 Place the small provided O ring in the mounting groove which is found on the valve block as illustrated Then move Inspira s white plunger back by turning the coupling connecting the black lead screw to the motor s
36. ing Commands with Input Data Format command data lt cr gt Response lt cr gt lt lf gt prompt SET RAT nnn Sets respiration rate in BPM breaths per min SETVOL nnn nn Sets tidal volume in cc SETIE nn Sets E Ratio as inspiration SETSTV nnn nn Sets sigh tidal volume in cc SETSFREQ nnn Sets sigh frequency as 1 breath per nnn breaths SETPRS nn Sets inspiration pressure limit in cmH50 SETWGT nn nnn Sets animal s weight in kg SELMOD mode Selects operation mode mode VOL or ASST Commands with Input Data Inspira ASVp Only Format command data lt cr gt Response lt cr gt lt lf gt prompt SETPRS nn Sets inspiration pressure in cmH20 Pressure control mode or pressure limit in cmH20 Volume control mode SET SPRS nn Sets Sigh pressure in cmH 20 Pressure control mode only SET PEEP nn Sets PEEP pressure in cmH20 SELMOD mode Selects operation mode mode VOL VOLASST PRES or PRESASST Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Appendix C OB ooo0o0Gnononoo Queries Format query lt cr gt Response lt cr gt lt lf gt value lt cr gt lt lf gt prompt RAT VOL IE STV SFREQ PRS MOD WGT VER AIRPRS FLT 00 01 02 03 04 05 Return respiration rate in BPM breaths per min as integer value Return tidal volume in cc to 2 decimal places Return E Ratio as inspiration Return Sigh Tidal Volume in cc to 2 decimal places Return Sigh Frequency as 1 breath per xxx breaths Ret
37. irculated for rebreathing collected and or filtered if chemicals are present in the expired air CAUTION Do not vent anesthesia gases to room air Source Flow of gas used by Inspira for inspira tion Connect to the source of inspira tion gas or leave disconnected to use room air Side Stream Not used in this model CAUTION Do not con nect Inspira to pressur ized gas sources unless proper pressure regulation is pro vided to equalize the pressure with atmospheric pressure Failure to do so could result in excessive and unknown tidal vol umes delivered to the animal Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Installation 9 7 0T Advanced Ealeiy Vaertilatar Fam Anien Bee Figure 4 Front Panel Electrical Connections Front Panel Inspira provides four BNC type connectors at the front of the machine Their func tions are as follow s Sync Out Produces a digital pulse at the start of the respiration cycle allowing synchronization of other electronic equipment with Inspira s respiration cycle Trigger In For use with equipment which uses a digital signal to start and stop respiration An example of this type of equipment would be a footswitch Analog Out 1 An analog signal representing the airway pressure within the ventilator This signal may be monitored with an oscilloscope Analog Out 2 Not used in this model Note Electrical specifications for these signals are give
38. l User Override The user has the option of overriding the machine s Safe Range limits to allow for more advanced respiratory control The Safe Range is defined as 10 of the machine s recommended tidal volume and 10 of the machine s recommended respi ration rate When you would like to change a respiration parameter simply enter the value according to the steps provided in the Getting Sarted section If the value is out side of the Safe Range Inspira will ask you if you would like to override the current Safe Range parameter On this prompt screen press 1 to select Yes or 2 for No Note Some parameters may not be valid due to current respiration profile cylinder size and electromechanical limitations A WARNING Overriding Safe Range may be harmful to the patient Key Display OUT OF RANGE OVERRIDE 1 Y 2 N Safe Range prompt Overrides Safe Range or 49 Maintains Safe Range Using Digital and Analog I O Ports Trigger In This is an active low digital signal used to trigger the ventilator between the RUN and STOP modes Each pulse given to Inspirathrough this channel will toggle the machine to the next state This optional signal is input by the user through the use of a footswitch or other device connected to the front panel BNC connector Minimum input pulse width 1 ms Sync Out Inspira ASVy This is an activedow open collector digital signal generated at the start of e
39. lumes of 0 1 to 100 cc It is designed for use in atypical laboratory operating room or any other well ventilated nonexplosive envi ronment The Inspira can be used to deliver all types of nonexplosive gas mixtures including but not limited to anesthetic gases and 100 oxygen Installation 9B LLL i VENTILATOR CONNECTIONS The InspiraASV comes with connection ports located on the front and rear panels of the ventilator These connections are shown on the following pages Galata Galette Werdiiator HT AiP a LINE T Area Lil id Biome Animal Input From Animal Source To Exhaust Side Animal Stream Expired Air To Animal Y Connector close to animal Tracheal Cannula or Endotracheal Tube Figure 3 Airway Tubing Connections Airway Tubing Connections Front Panel The InspiraASV provides four airway tub ing connections at the front of the machine A fifth port Side Stream is pro vided to support advanced options These ports are standard 1 8 27 NPT threaded ports and may accept many types of fittings including barbed and luertock This allows for a wide range of tubing sizes and for accommodating air filters if needed The functions of these connections are as follows To Animal Flow of source gas from ventilator to a From Animal Flow of expired gas from animal to ventilator Exhaust Flow of expired air from ventilator This gas may be vented to room rec
40. me for inspiration and expiration phases Adjustable I E Ratio This option allows the user to select the ratio of inhalation to exhalation times when advanced respiratory control is needed This feature is intended to allow greater res piratory control for research The default I E Ratio is 50 Flow Rate Monitoring Future Feature Flow rate monitoring will allow continuous monitoring of the rate at which the gas is being delivered to the animal This feature will also aid respiratory research Programmable Sigh Breath Continuous long term ventilation combined with the force of gravity will cause the animal s lungs to begin to collapse Introducing a larger than normal tidal volume overinflates the animal s lungs replicating a natural sigh It allows the lungs to expand and opens the collapsed alveoli Sigh breaths are supported in both modes In the volume controlled mode a sigh tidal volume is used In the pressure controlled mode a sigh pressure limit is used The frequency and volume of the sigh breath are user selectable in volume controlled mode In the pressure controlled mode the fre quency and pressure of the sigh breaths are selectable Respiratory Assist The Inspira ventilator supports Respiratory Assist in both volume and pressure con trolled modes This feature is used to wean an animal off the ventilator When the ani mal attempts to initiate a breath on its own a pressure sensor detects the drop in air way pressure
41. n in Appendix A Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Installation DO Electrical Connections Rear Panel Inspira provides power entry RS232 com munications and User I O Optional con nections at the rear of the machine Their functions are as follow s User 1 0 Not used in this model RS232 In For use with a host computer to establish a communication connection between the computer and the Inspira Figure 5 Rear Panel 9 po OY Gr oe ee E ES No connection Transmit Data TXD Receive Data RXD DataTerminal Ready Input DTR 4 Signal Ground GND No Connection Request to Send Input RTS No Connection No Connection 1 Used for Vpp Enable in optional flash programming configuration disabled in hardware 2 Used as Serial Reset in optional flash programming configuration disabled in hardware RS232 Out Used to establish a daisy chain serial connection with other devices Future models will support a serial printer connection through this port 1O Qo es SO UD oe ctor ONU qe No connection Receive Data RXD Transmit Data TXD No Connection Signal Ground GND No Connection No Connection No Connection No Connection Power Connection Power in Note Electrical specifications for these signals are given in Appendix A Installation DO RI Large Air Cylinder Small Air Cylinder Front Switch Rear Switch White Plunger Flag P
42. nter Sigh Freq 1 Sigh Breaths Enter Sigh Freq 1 Sigh 12 Breaths Operation Respiration Profiles 31 EEE Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Operating Modes The Inspira ASV has two operating modes Volume Mode and Assist Mode To toggle between the modes press the Select Mode key The upper right hand corner of the display will show which mode the ventilator is currently in either VOL or ASST The Inspira ASVp has four operating modes VOLUME VOLUME ASSIST PRESSURE and PRESSURE ASSIST To toggle between the modes stop the machine by pressing the RUN STOP key if it is presently running and then press the SELECT MODE key When the mode is switched from VOLUME ASSIST to PRESSURE or PRESSURE ASSIST to VOLUME the machine will reinitialize to place it in its home position for that mode The upper right hand corner of the display will show which mode the ventila tor is currently in VOL VOLASST PRES or PRSASST Volume Mode When in Volume Mode the ventilator delivers a known volume of gas to the animal over a specified amount of time for each respiration stroke This is the basic operating mode for volume controlled ventilation Pressure Mode Inspira ASVp Only When in Pressure Mode the ventilator delivers gas until the desired airway pressure is reached or the end of the inspiration cycle has occurred If the airway pressure is not reached within the inspiration time the machine will automa
43. ntering Tidal Volume rr 22 Entering Sigh Breath ii 23 Disabling Sigh Breath sse 23 Entering EE Ratio euet etes 25 Entering Pressure Settings Inspira ASVv Inspiration Pressure eene 26 Assist PreSSUI GO sai eir Debs 27 Entering Pressure Settings Inspira ASVp Inspiration Pressure eene 28 ASSIS PrOSSUIO iot eie terae nes 29 PEEP Press te iei AN N 29 Sighi Pressure eei tnter retten 30 Table of Contents 2 mE LLL Modes of Operation Volume ModE iene 31 5 Pressure Mode cisl es 31 Assist Mode itin rettet etd 31 S Advanced Operation ta User Override sss 32 8 Using Digital and Analog I O Ports 32 7 Remote Control Monitoring Serial Communications 33 Changing Air Cylinders i 36 HardWare Setup cai 37 c DiagNOStC S Mem 39 Power Failure os alia aiar 44 General Maintenance and Cleaning 44 Appendices 11ssrrrserie irene eine nine ieri rien 45 56 A General Specifications sssseee 45 B VO Specifications teet ette ee 46 C Serial Commands Queries and Responses 47 48 D Glossary of Terms alii 49 E Sample Waveforms ccccccecceeeesecececeeeeeeeeseeeeeaeeneeteees 50 E Frequently Asked Questions in 51 52
44. o verify respiration parameters If any parameters need to be changed press the SET key and the appropriate parameter key and enter the value to set Press and release the RUN STOP key to start ventilation Pressure Controlled Ventilation Inspira ASV Only With machine powered ON press and release the SELECT MODE key until PRESmode is selected Press SET and WEIGHT and enter the animal s weight in kg Press and hold parameter keys in order to verify respiration parameters If any parameters need to be changed press the SET key and the appropriate parameter key and enter the value to set Press and release the RUN STOP key to start ventilation Operation Getting Started AT ZZZ Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Main Operating Screens The main operating screen is the default display This screen displays the operating mode current operating variables such as tidal volume and respiration rate and the status of the ventilator stopped or running Main Operating Screen Pressure Control Mode Paw XX cmH 0 PRES Rate XXX BPM R gt gt gt gt Main Operating Screen Pressure Assist Control Mode Paw XX cmH 0 PRSASST Rate XXX BPM R gt gt gt gt Main Operating Screen Volume Control Mode Vt XXX XX cc VOL RATE XXX BPM R gt gt gt gt Main Operating Screen Volume Assist Control Mode Vt XXX XX cc VOLASST RATE XXX BPM R gt gt gt gt Paw Average Peak Pressure in cmH20 Vt
45. ostics Press 5 to enter the diagnostics menu The display should read Press 1 Dsply 2 Key 3 Alarm 4 Next Step Key Display a O Advances to the next menu PRESS ADRIA sell 5 9 3 ALARM 4 NEXT Enters the diagnostic menu Display Diagnostics To perform the display diagnostic enter the diagnostic menu Press 1 to perform the display test The display should read Display Test then illuminate all pixels and return to the diagnostics menu Stept Key Display 6 Performs display test Keypad Diagnostics To run the keypad diagnostic enter the diagnostics menu Press 2 to start the key pad diagnostic The display should momentarily read Keypad Test Press Enter to Exit Then the display will show acursor in its upper left hand corner This test echoes key strokes on the display Press each key and verify that the display echo matches the key pressed Press Enter to exit this test and return to the diagnostics menu Stept Key TETTE KEYPAD TEST 6 9 Performs keypad test al de Exits keypad test mode Note See page 37 for steps 1 through 3 T Note See above for steps 4 and 5 Operation Advanced 0 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Audible Alarm Diagnostics To test the audible alarm enter the diagnostics menu Press 3 to perform the alarm test The display should read Press set to test Press enter to exit Press the Set key to
46. peration Advanced GO 0000 Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Data Transfer Rate Configuration To configure the data transfer rate enter the port configuration menu Press 1 to enter the Baud rate setup screen The display should read Press Select Mode to tog gle baud and display current baud rate Press the Select Mode key to toggle through the baud rates 38400 19200 9600 2400 and 1200 Pressing the Enter key will accept the valve and return Inspira to the main screen Default baud rate is 9600 Step Key Display 5 PRESS SELECT MODE TO TOGGLE BAUD xxxxx Enters baud rate setup screen 6 Select PRESS SELECT MODE TO Mode TOGGLE BAUD xxxxx Toggles baud ZI Accepts value and returns to main screen Start Word and Stop Bit Configuration To change the bit configuration enter the port configuration menu by pressing 2 The display should read Define Serial Bits STRT WD STP and display the current bit settings respectively The start bit is fixed at 1 bit and word bits are fixed at 8 Use the numeric keypad to change the number of stop bits Press Enter to return to the main Screen Step Key Display 2 2 DEFINE SERIAL BITS f STRT WD STP ly o X Enters bit setup screen 6 DEFINE SERIAL BITS STRT WD STP ih bol Changes stop bits to 1 2 DEFINE SERIAL BITS SHR S WD SP ly 9 2 Changes stop bits to 2 VCS Accepts entry and
47. reater than flow out of bag Gas pressure must be equalized with atmospheric pressure before drawing into ventilator An optional bleed circuit may be used to prevent pressurization of breathing bag Appendix J 56 MEE LLL Generating PEEP with Inspira ASV lt O O Air Gas From Source Input Animal To Animal Exhaust Side Stream ZN Expired Air Gas eww PEEP no cmH20 yf Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Tracheal Cannula or Endotracheal Tube WARNING IF USING ANESTHETIC GASSES SCRUB OR VENT THE EXPIRED GAS DO NOT VENT TO ROOM AIR
48. returns to main screen or Note See page 33 for steps 1 through 4 Operation Advanced FS I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Parity Configuration To configure the port parity settings enter the port configuration menu Press 3 to enter the parity configuration menu The display should read Define Parity 1 Even 2 Odd 3 None Choose the desired parity by pressing the corresponding numer ic key After making your selection Inspira will automatically return to the main screen The default parity is none Step Key Display 5 8 DEFINE PARITY x 2 1 EVEN 2 0DD 3 NONE Enters bit setup screen Changes parity parameter and returns to main screen Address Configuration Future Feature To assign an address to the Inspira for use in a daisy chain enter the port setup menu Press 4 to enter the address configuration menu The display should read Enter Address and display Inspira s current address Use the numeric keys to enter an address between 0 and 999 Press Enter to enter the selected address and to return to the main screen Step Key Display 2 Enters address setup screen 8 O0 108 Selects address ZI Enters address and returns to main screen Testing RS232 Communication Once Inspira and the external device are configured with the same port settings con nect the Inspirato the external device using the proper cable Send theVER command from
49. rm This fault indicates that a vacuum trigger which is generated by an inhalation effort by the patient has not been detected on 5 consecutive cycles This would be a result of the patient failing to initiate breaths on its own or of improper respiration parameter settings such as assist pressure threshold or respiration rate Airway Under Pressure Underpressure Alarm This fault indicates that an under pressure condition has been detected in the airway For Volume Control mode an under pressure condition is defined as a pressure less than 25960f the average measured peak pressure averaged over 10 samples not count ing sigh breaths checked at a time approximately halfway into the inspiration stroke This could indicate that the airway is not connected or is leaking This is a critical fault and requires operator intervention The machine shall continue to run at the current respiratory settings This fault shall be self clearing once the condition is corrected The user is always notified via a visual alarm and also by an audible alarm if enabled Underpressure Alarm Inspira ASVp Underpressure Alarm If the average airway pressure is less than 3cmH20 an underpressure alarm is report ed Underpressure conditions are generally caused by severe system leaks This alarm is self clearing on removal of the fault condition Initialization Fault Initialization Fault This fault indicates that the machine failed to initialize properly
50. rmation Thelnspira can handle animal weights ranging from 0 015 to 10 00 kilo grams Thelarge cylinder can handle masses between 0 160 and 10 00 kilograms and the small cylinder can handle masses between 0 015 and 0 59 kilograms Entering an animal s weight will allow Inspira to automatically choose a Safe Range tidal volume and respiration rate No extra setup is required To enter the animal s weight press the Set key The display should now read Press parameter key Press the Weight key The display will now read Enter Weight Kg Simply use the numeric keypad and decimal point key to enter the proper weight between 15 g and 10 kg The weight must be entered in kilograms Inspira will allow for the entry of up to three decimal places After entering the weight press the Enter key The display should read Change SV and BPM stroke volume and breaths per minute Use the Set key to move the bar cursor to select either yes or no Press Enter to select your choice Selecting yes will change the SV and BPM to the auto matically calculated Safe Range ventilation settings Selecting no will leave the pre viously used SV and BPM settings which may not be safe for the animal Step Key Display 1 Press Parameter Key 2 Enter Weight kg Enters weight configuration mode 3 00000 ME Selects weight Yes No 4 Change SV and BPM Enters weight Yes No 5 Change SV and BPM Moves cursor to select
51. s Default No Sigh Insp Pressure to 50 cmH20 Insp Pressure to Insp Pressure 10cmH20 or 35cmH20 whichever is less Available 1 to 10 cmH 0 Default 5 cmH 0 0 to 5 V digital pulse active low 0 to 5 V digital pulse 20 10 ms wide active low Voy for Inspiration Duration Vo for Expiration Duration 0 to 5 V analog Default Airway Pressure Signal 67 cmH50 50 mm Hg 30 mV cmH 70 typical 40 0 mV mmHg typical 40 5 to 4 5 V 42 5 V 30 05 V 1 ms max 0 to 45V analog Default Not Used RS232 2 line x 20 character Vacuum Fluorescent Display VFD AC 100 to 240 V 50 60 Hz 75 watts 17 8 x 25 4 x 40 6 cm 8 x 10 x 17 25 in 9 1 kg 20 Ib Appendix B J LLL Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual 1 O Specifications Specifications Analog Out 1 Analog Out 2 Signal Type Analog Output Vou max 10 5 V Ry 1 Mohm Vo typical 0v VoL max 50 mV Ig 0 IOUT 0 5 mA Vo 5V R 10 kohm lour max 30 mA Protection Short Circuit Duration Indefinite ESD Protected Connector Type BNC Cable Length 3 m max Sync Out Signal Type Digital Output Vou typical 5V Von min 2 5V Q Igy 225 mA VoL max 0 3 V lg min 1 mA loi typical 15 mA Pulse Width 20 ms 10 ms Inspira ASVy Only Pulse Width Inspira ASV VOH br Inspiration Duration Vo for Expiration Duration Protection Short Circuit Duration Indefinite ESD Protected Connector Type BNC Cable Leng
52. sure is 5 cmH 0 TheASv will always use a negative assist pressure To set the assist pressure press the Set key followed by the Pressure key The display should now show alist of options Press 1 Insp 2 Asst Press 2 on the numeric key pad to select the assist pressure Now the display should read Enter Assist Pressure and also show the current assist pressure setting Use the numeric keypad to enter the vacu um pressure the negative sign does not need to be entered Inspira assumes that this value is negative Press the Enter key to enter the selected assist pressure value Step Key Display 7 set Press Parameter Key 2 PRESS 1 I NSP 2 ASST Enters pressure configuration mode 3 2 Enter Asst Pressure D cm H20 Enters assist pressure setup 4 Enter Asst Pressure 5 cm H20 Selects assist pressure 5 amp Enters assist pressure and returns to main screen Operation Respiration Profiles 28 mE C Entering Pressure Settings Inspira ASVp Inspiration Pressure Step Key Display 1 Press Parameter Key 2 Aur Enters pressure configuration mode 3 O Enter Insp Pressure _ cm H20 Enters inspiration pressure setup 4 OOo Enter Insp Pressure 30 cm H20 Selects inspiration pressure 5 C2 Enters inspiration pressure and returns to main screen INSP 2 ASST PEEP 4 SIGHi Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Note 1 Sigh
53. sure to Insp Pressure 10cmH20 or 35cmH20 whichever is less Full Range Insp Pressure to 50cmH20 Operation Getting Started 17 EEE Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Ventilator Graph The graph labeled as figure 11 below shows tidal volume vs body mass and rate for labo ratory mammals in a resting state Apparatus dead space must be added 70 100 200 300 400 600 800 1 2 3 4 56 8 10 15 20 30 40 500 500 400 400 300 LIL 1 J 300 200 200 150 150 o I a 100 a ie LLLA LA 1400 n LL Jo rn Z EIE as E 60 a i 1 Tf 60 o 50 Breaths per Minute 10 50 E Z 40 40 40 c 30 5 of L LLL 15 50 E oft 0 LIT eo I ign Pai 15 20 15 oO i 25 E 10 60 10 8 30 70 8 90 3 5 5 4 4 100 3 3 2 m 2 2 40 1 t 0 8 0 8 0 6 0 6 0 5 0 5 0 4 0 4 70 100 200 300 400 600 800 1 2 3 4 56 8 10 15 20 30 40 Grams Body Mass Kilograms Figure 11 Ventilator Graph Ventilation Parameters Mammals with 70g Body Mass Body Mass g Vt ml Rate BPM 20 0 12 148 30 0 18 133 40 0 24 123 50 0 30 117 60 0 36 111 70 0 42 107 Operation Getting Started DB 12 LLLLLLLLLLLI Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Entering Animal Weight Key Info
54. th 3 m max Trigger In Signal Type Digital Input Input pulled up to 5 V through a 330 Q resistor Drive with open collector or switch closure Vig min 24V Vin max 1V Vip max 0 8 V lj typical 13 5 mA Pulse Width 1 ms minimum Protection Short Circuit Duration Indefinite ESD Protected Connector Type BNC Cable Length 3 m max RS232 Input Output Signal Type Digital Input Output to EIA RS232C Standard Protection Short Circuit Duration Indefinite ESD Protected Connector Type 9 pin D Sub Cable Length 3 m max Appendix C 47 UT Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual SERIAL COMMANDS QUERIES AND RESPONSES Each command sent to the Inspira is a string of ASCII characters Commands are not case sensitive and spaces are optional After a transmission to the Inspira terminating with a cr character the Inspira enters remote mode and responds with the three character sequence lt cr gt lt lf gt prompt lt cr gt carriage return ASCII 13 decimal lt f gt line feed ASCII 10 decimal Prompts Stopped ASCII 58 decimal gt Running ASCII 62 decimal Fault ASCII 42 decimal The following symbols are used in describing the commands Commands Format command lt cr gt Response lt cr gt lt lf gt prompt KEY Return to keyboard control exit remote mode RUN Start ventilation STP Stop ventilation SIGH Commands SIGH Breath OVRD Override Safe Range checking SAFE Enable Safe Range check
55. the external device to Inspira If the RS232 connection is working properly the Inspira will return its version number If nothing is returned check the cable con nection and the port settings to verify that they are configured correctly Note See page 33 for steps 1 through 4 Operation Advanced FO I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Changing Air Cylinders The Inspira ventilators use two easily interchangeable piston cylinder assemblies These assemblies are available to provide 0 1 to 10 ml and 1 to 100 ml tidal volumes Changing the piston cylinder assembly is simple Remove the top cover of the venti lator by removing the seven phillips head screws which hold the top cover in place Once the top cover is removed locate the currently installed cylinder Move Inspira s white plunger back by turning the coupling connecting the black lead screw to the motor shaft Turn the lead screw until the flag pin on the rear of the plunger is verti cally indine with the rear switch as seen illustrated below Remove this cylinder by unscrewing it from the valve block Next carefully remove the cylinder from the Inspira chassis Place the O ring from the extracted cylinder back into the mounting groove which is found on the valve block Note When installing the air cylinder do not change the bends of the cylinder detection switches or else the correct cylinder will not be detected Now insert the new cylinder
56. tically adjust the inspira tory flow rate by incrementally increasing the tidal volume until the pressure limit is achieved If the pressure limit is reached early in the inspiratory cycle the machine will automatically decrease the flow rate so that the limit is reached toward the end of the cycle If the limit is reached very early in the cycle the machine will also report an Occlusion Fault Volume Assist Mode Pressure Assist Mode Inspira ASVp only Assist mode is a more advanced mode used to help wean animals off of mechanical respiration This mode detects when the animal is trying to breathe on its own via a drop in the airway pressure This mode allows the animal to initiate the respiration strokes The ventilator starts a respiration stroke when the airway pressure falls below a user defined threshold If five respiration strokes occur without the airway pressure dropping below the assist pressure threshold the animal may not be trying to initiate a breath The ventilator will continue to operate in the current assist mode but the assist alarm will sound to request user intervention The user may adjust the respira tion parameters and or the assist pressure to obtain correct performance CAUTION Assist Mode should only be used when trying to wean animals from mechanical ventilation Key Select Mode Toggles between available modes Operation Advanced Fo I Harvard Apparatus Inspira Advanced Safety Ventilator User s Manua
57. tly available Pressing 2 selects the Analog I O menu The display should now read Analog 1 Arwy Pres This indicates that the airway pressure ana log signal is connected to Analog Out 1 This is the only analog signal available in this model Inspira then returns to the main display screen Step Key Display SET I 0 PRESS E 2 1 DIGITAL 2 ANALOG Enters I O configuration 5 Displays current analog setting and returns to the main screen Display Brightness Level To change the display brightness level enter the hardware configuration menu Press 3 to enter the display configuration The display should now read Set Brightness Level 100 default setting There are four options for the display brightness 25 50 75 and 100 Use the Select Mode key to toggle through the different levels of brightness The enter key selects the brightness level and then returns to the main Screen Step Key Display SET BRIGHTNESS LEVEL 4 O Enters display brightness configuration Select SET BRIGHTNESS LEVEL DI Toggles to the next brightness level 6 Accepts value and retums to main screen Note See page 37 for steps 1 through 3 Operation Advanced FO ML Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Diagnostics To enter the Inspira diagnostic menu first enter the hardware configuration menu and press 4 to advance to the next menu The display should read Press 5 Diagn
58. ual Terminal Introduction Sf Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Figure 1 Front view of Figure 2 Rear view of Inspira Advanced Safety Ventilator Inspira Advanced Safety Ventilator Theory of Operation The Inspira is Harvard s first ventilator that can ventilate animals from mice to cats using the same machine Its versatility and ease of use allow safer and superior ven tilation The InspiraVentilator uses an advanced piston cylinder assembly and a micro processor controlled actuation mechanism to precisely control respiration profiles Two interchangeable piston cylinder assemblies are available to provide a wide range of tidal volumes The ventilator auto detects which cylinder or if no cylinder is installed Variable flow valves control the gas flow for inhalation and exhalation The Inspira ASV Volume Controlled Ventilator delivers the desired tidal volume to the animal by precisely controlling the stroke of the piston Since actual stroke length and therefore tidal volume may be modified for a given stroke sigh breaths are sup ported Since stroke speed is precisely controllable during inspiration and expiration variable inspiration to expiration ratios are also supported A pressure sensor contin uously monitors the airway pressure to prevent over pressure conditions Most vol ume controlled ventilators presently available do not support sigh breaths and do not provide any pressure monitorin
59. urn inspiration pressure limit in cmH 20 Return operation mode VOL AST Return animal weight in kg to three decimal places of precision Return model ASVv ASVp ARV and firmware version number Returns airway pressure in cmH50 Returns a 2 digit fault code xx defined as follow s No Fault Overpressure Under pressure Motor Drive Fault Pump Stall Valve Stall Queries Inspira ASVp Only Format query lt cr gt Response lt cr gt lt lf gt value lt cr gt lt lf gt prompt Value is a string of characters of varying length PRS SPRS PEEP MOD BSLNPRS AVGPRS Returns requested inspiration pressure Pressure control mode or pressure limit Volume control mode in cmH20 Returns requested sigh pressure Pressure control mode only in cmH20 Returns requested PEEP pressure in cmH20 Returns operation mode VOL VOLASST PRES or PRESASST Returns actual baseline pressure EEP in cmH20 Returns actual average peak pressure in cmH20 Error Responses lt cr gt lt lf gt lt cr gt lt lf gt prompt Unrecognized command lt cr gt lt lf gt O0R lt cr gt lt lf gt prompt Out Of Range Appendix D Ap 0G n0 0 Gnto0on Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Glossary of Terms Assist Respiratory assist mode w hereby a subject is aided in regaining normal breathing after artificial respiration weaning or for respiration of a non paralyzed anesthetized sub ject Ventilation is triggere
60. ws the fault This indicates that the residual volume may be increasing in the animal s lungs and user intervention is required immediately Ventilation will still occur after this threshold has been broken The default Inspiration Pressure is 35 cmH20 To set the inspiration pressure press the Set key followed by the Pressure key The display should now display a list of options Press 1 Insp 2 Asst Press the 1 on the numeric keypad to select the inspi ration pressure The display should now read Enter Insp Pressure and display the cur rent pressure setting Using the numeric keypad enter the inspiration pressure setting in cmH 0 Inspira will only accept whole number values for this setting Press Enter to enter the selected pressure setting Step Key Display 1 Press Parameter Key 2 Enters pressure configuration mode 3 Enter Insp Pressure 5 cm H20 Enters inspiration pressure setup 4 OC Enter Insp Pressure 38 em hz Selects inspiration pressure 51 Enters inspiration pressure and returns to main screen Operation Respiration Profiles OT ML Harvard Apparatus Inspira Advanced Safety Ventilator User s Manual Assist Pressure The assist pressure is a sub baseline pressure threshold which is used to trigger an inspi ration stroke Inspira looks for this trigger within the first 1096 of the calculated inspira tion time which is determined by the inspiration rate The default Assist Pres

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