User Manual 625FS
Contents
1. AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FS as sss sss o O O C W User Manual Version 1 2 2 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FS USER MANUAL TRADEMARKS DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing New information may be supplied separately This documentation is provided with the DMT Automatic Buffer Filler System Model 625FS All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as required and without any notice Copyright Danish Myo Technology A S DANISH MYO TECHNOLOGY TRADEMARKS 3 CONTENTS Automatic Buffer Filler System aio 3 TE uu uuu c cee A 3 CL AA PPP A A 5 A APP O o yu n a E EN 6 EMO EMD eec ken ki a l 7 PDO ANS ki u u uuu DO Do oc 7 Certificate of Conformity ee o 8 ABOUT UNIS MAN WAN e 9 Unpacking the Automatic Buffer Filler System G25FS J U U U U2. U U IDs RR RR u T T T T J 10 Chapter 1 System overview U dcc vU SEU u u kk EDD NR EN GIO BE 11 1 1 Automatic Buffer Filler System 625FS front panel a a m ti kk RA 11 1 2 Automatic Buffer Filler System 625FS rear panel in 11 Chapter Setting Up ti it fit ARA OO en a a a a e me 12 2 1 Complete Automatic Buffer Filler System 625FS onet 12 Fi SOREN up SOP Dy STOD sitiada a a a it 13 2 3 Connect 625FS buffer outlet totheTOoUrechsmbbef Sas kt ta ki tk ai a tt lk RN 16 UIO SS GON SIS riral uuu n lt n aaa ina 17 Chapter Use of The Automatic Buffer Filler System 625FS J J J U U U U u u Q T T 18 3 1 General use of the Automatic Buffer Filler System 625FS a 18 Chapter 4 Maintenance of Automatic Buffer Filler System 625FS J J J J naa aaa sana ann aaa ann 19 FA GE G Hier o u k fb ta ae Cat tn ti umum us emm 19 AZA OF G20 pelis MIS IET TOT 20 Appendix 1 Custom selected 625FS Filler volumes J U
3. e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different to that which the receiver is connected to e Consult the dealer or an experienced radio TV technician for help APPROVALS Complies with the EMC standards EN 61326 2 6 2005 EMC 89 336 EEC EN 61000 3 2 Certified with the safety standards Directive 2006 95 EC EN 61010 1 2001 EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 EMC EMI 7 8 EN 61010 1 2001 EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 EN 61326 2 6 2005 CERTIFICATE OF CONFORMITY DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Automatic Buffer Filler System Model 625FS is covered by this certificate and marked with CE label It conforms to the following standards Safety requirements for electrical equipment for measurement con trol and laboratory use Part 1 General requirements Safety requirements for electrical equipment for measurement con trol and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment Electrical equipment for measurement control and laboratory use EMC Requirements Part 2 6 Particular requirements In vitro diagnostic IVD medical equipment With reference to regulations in the following direc
4. enne annnm naa enn u u sanum uaa Ra sauna 21 Appendix 2 System Specifications J U U aaa IIIa RR RR uu u u u ERR sss RR S Q Q J 22 e e 23 4 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL INTRODUCTION All DMT 4 channel myographs systems the 610M 620M 700MO 720MO 800MS and 820MS have built in suction activated by the press of a button that empties the bath The user fills the bath again manually with pre warmed buffer from bottles with for example a pipette Manual filling presents a risk of inconsistency across the four baths effecting the accuracy of results There is a danger of knocking and damaging a transducer with a pipette tip Furthermore consideration should be given to the risk of repetitive strain injury particularly for those manually filling multiple four chamber systems in parallel To eliminate the above mentioned issues DMT has developed the Automatic Buffer Filler System 625FS The 625FS can be easily mounted on all our four chamber systems and makes it possible to fill one specific chamber or all chambers at once with pre selected volumes simply by pressing a button INTRODUCTION 5 SAFETY The Automatic Buffer Filler System 625FS has been designed for use only in teaching and research applications It is not in tended for clinical or critical life care use and should never be used for these purposes or
5. the pipe turning it 90 counter clockwise and gently lowering it into the chamber as shown in figure 2 10B below NOTE THE TRANSDUCERS ARE FRAGILE AND SENSITIVE TO MECHANICAL STRAIN BE VERY CAREFUL WHEN LOWERING THE STEEL PIPES INTO THE CHAMBER THAT THEY DO NOT HIT THE PINS OR JAWS IN THE CHAMBER These buffer inlet tubes should now be positioned close to the wall of the myograph chamber as possible Adjust the buffer inlet and gas supply pipes in such a way that they are close to the wall of the chamber and have a minimum effect on the mounted tissue It is important to position the inlet pipes in the same position in each chamber Figure 2 10 A B Stee pipe with silicone tube attached to vacuum pipe 16 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL 2 4 Chamber Covers The chamber covers that come with the 625FS figure 2 11 A have been modified to accommodate the additional buffer inlet The original covers that were supplied with your Multi Myograph System figure 2 11B should be set aside The covers help maintain temperature and other buffer conditions gas tension pH Holes in the chamber covers serve different purposes and they are illustrated in figure 2 12 Figure 2 11 A B 625FS chamber cover and 620M chamber cover Figure 2 12 Modified chamber cover for 625FS CHAPTER2 17 CHAPTER USE OF THE AUTOMATIC BUFFER FILLER SYSTEM 3 1 General use of the Automatic Buffer Filler System 625FS 1
6. 25FS lid on top of it 2 Place the lid with the steel pipes in the 8 acetic acid and press the recirculating button and let it run for 5 minutes And then stop the pump 3 Follow the return tube coming from the port marked 3 OUT on the back of the 625FS Detach the tube from the buffer flask lid and place it in a waste container 4 The flask lid is detached and placed in an empty bottle or just held up in the air Press the button to start the recirculation pump 5 After 30 seconas the buffer reservoir in the 625FS will be emptied of acetic acid 6 Place the flask lid in a container of distilled water for at least 3 5 minutes During this period fill and empty the chambers of the Multi Myograph three times with the larger volume button to clean the short tubing connecting the 625FS to the myo graph chambers 7 The buffer flask lid is again placed in an empty bottle or just held in the air After 60 120 seconds the 625FS is clean and empty IMPORTANT NOTE NEVER SOAK THE SUPPORTS IN ANYTHING STRONGER THAN 8 ACETIC ACID FOR EXTENDED PERIODS OF TIME DO NOT SOAK FOR HOURS OR OVERNIGHT 20 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL APPENDIX 1 CUSTOM SELECTED 625FS FILLER VOLUMES If you prefer different filling volumes to the two default volumes DMT will need the 625FS returned for setting and calibration DMT will set the new volumes within one week and return the 625FS to you APPENDIX1 21 APPEN
7. Connect all the tubing as described in Chapter 2 and place the buffer flask of up to 700ml of buffer in a pre heated water bath Try to keep the tubing between the buffer flask and the 625FS as short as possible Activate the re circulating button at least 5 minutes before use to ensure that the reservoir inside the 625FS is filled with buffer that is very close to the temperature of the water bath The re circu lating button activates a pump that will re circulate the buffer from the flask through the 625FS and back to the flask The re circulating pump has a high flow rate to ensure that the temperature in the reservoir will re main constant IMPORTANT THE RE CIRCULATION BUTTON MUST BE ACTIVATED AT LEAST 5 MINUTES BEFORE USE OF THE FILL ER FUNCTION AND NOT DEACTIVATED BEFORE THE END OF THE EXPERIMENT 4 A specific chamber can be filled by pressing the corresponding button for that chamber on the 625FS with either the larger volume the lower button or the smaller volume upper button All four chambers can be filled simultaneously by pressing the all button for either the larger or the smaller volumes WARNING REMEMBER TO EMPTY THE CHAMBERS BEFORE RE FILLING OTHERWISE THEY WILL OVERFLOW AND RISK HARMING THE MYOGRAPH INTERFACE 18 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL RE CIRCULATION LARGE VOLUME SMALL VOLUME ALL ALL CHAPTER 4 MAINTENANCE OF AUTOMATIC BUFFER F
8. DIX 2 SYSTEM SPECIFICATIONS Technical specifications Filling volumes Custom volume range Filling speed Fill time 8ml Fill time 5ml Voltage Power supply Current max Environmental humidity Environmental operating temp Environmental storage temp Compatible with 6 ml and 8 ml 0 5 ml 4 10 ml 30 ml min typical 15 sec typical 10 sec 100 to 240 VAC auto 50 60 Hz via external power supply 24VDC 10 1 0Amps 20 to 80 RH non condensing 15 30 C 4 TO C 610M 620M 700MO 720MO 800MS 820MS systems 22 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL NOTES NOTES 23 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China Tel 86 0 21 5425 1330 Fax 66 0 21 5377 0063 www dmt asia com sales dmt asia com support dmt asia com DMT USA Inc 201 East Liberty Street Suite 6 Ann Arbor MI 48104 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
9. ILLER SYSTEM 4 1 Cleaning the 625FS DMT strongly recommends that the 625FS is cleaned after each experiment or at the end of the day using the following proce dure 1 Follow the return tube coming from the port marked 3 OUT on the back of the 625FS Detach the tube from the buffer flask lid and place it in a waste container 2 The buffer flask lid is detached and placed in an empty bottle or just held up in the air as shown in figure 4 1 below Figure 4 1 Position of the lid and steel pipes to empty tubes and filler reservoir of buffer 3 Press the button to start the recirculation pump 4 The buffer reservoir in the 625FS will now be emptied into the waste container After 30 seconds the buffer reservoir will be empty 5 Place the buffer flask lid in a container with distilled water for at least 120 seconds During this period fill and empty the chambers of the Multi Myograph three times using the larger volume button to clean the short tubing connecting the 625FS to the myograph chambers 6 After 120 seconds of soaking with distilled water the buffer flask lid is again placed in an empty bottle or just held up in the air After 60 120 seconds the 625FS is clean and empty CHAPTER4 19 4 2 Cleaning of 625FS Reservoir for salt Routinely implement a cleaning procedure for the 625FS with 8 acetic acid to remove salt inside the reservoir chamber 1 Prepare 500ml 8 acetic acid in the 1 liter flask Place the 6
10. ange circles in figure 2 3 below Figure 2 2 Complete Multi Myograph System 610M with the Automatic Buffer Filler System 625FS 2 J V L Mu OXYGEN N A f 5 V 6 fo 5 cp o w TRANS 4 REC 4 REC 3 REC2 REC 1 TRANS 3 TEMP PROBE RS 232 TRANS 2 RS 485 RS 485 TRANS 1 Figure 2 3 Fastening of the 625FS system onto the interface by tightening the two screws marked with orange circles CHAPTER2 13 2 2 2 Mounting 625FS onto a 620M 720M0 820MS system DMT ufe Q Empty valves Figure 2 4 Multi Myograph System 620M with the Automatic Buffer Filler System 625FS mounteq Unplug the power cord from your interface Remove the four chambers from the interface Place the 625FS s two legs onto the back edge of the heating plate of the interface Align the four screw holes of the legs of the 625FS with the four screw holes on the back of the heating plate of the interface figure 2 5 below Use the supplied four screws and the Allen Key to fasten the 625FS securely to the interface WWWDMTDK C HO T IP SX Figure 2 5 Fastening of the 625FS system onto the interface by tightening the two screws marked with orange circles 14 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL 2 2 3 Buffer flask connection to the 625FS e Place the black lid with the three steel pipes onto the supplied buffer flask figure 2 G e Use the
11. for the prevention diagnosis curing treatment or alleviation of disease injury or handicap Do not open the unit the internal electronics pose a risk of electric shock To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus Install in accordance with the manufacturer s instructions Do not install near any heat sources such as radiators heat exchangers stoves or other equipment or devices that produce heat Only use attachments and accessories specified by the manufacturer Unplug this apparatus during lightning storms or when unused for long periods of time This apparatus must be grounded Use a three wire grounding type cord similar to the one supplied with the product Do not modify the polarized or grounding type plug A polarized plug has two flat blades one being wider than the other A grounding type plug has two blades and a third round grounding pin The wide blade or the third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet Be advised that different operating voltages require the use of different types of line cord and attachment plugs Check the voltage in your area and use the correct type See the table below Voltage Line plug according to standard 110 125 V UL81 and CSA C22 2 No 42 220 230 V CEE 7
12. ist to check that the system is complete e One 625FS Buffer Filler unit e One power cord e Silicone tubing One 1litre buffer bottle One lid for the 1 litre bottle with three steel pipes two for the recirculation of the buffer and a third for applying gas to your buffer One screwdriver for mounting the 625FS onto your Multi Myograph System e One CD with user manuals for Wire Myograph Systems The shape of the AC plug varies by country be sure that the plug fits the outlets for your location 10 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL CHAPTER 1 SYSTEM OVERVIEW 1 1 Automatic Buffer Filler System 625FS front panel Power indicator m esoPy t p snn In a v y a Silicone tubes with steel pipes Corresponding buttons Re circulating button Figure 1 1 Automatic Buffer Filler System 625FS front panel On the front of the 625FS you have two rows of filling buttons figure 1 1 above ws And far to the right a button to activate the pump and purging of the 625FS system with buffer The top row is the buttons for filling specific chambers with the smaller volume and the bottom row is for filling specific chambers with the larger volume To the far right the button marked is used to fill all four chambers simultaneously with either of the two volumes 1 2 Automatic Buffer Filler System 625FS rear panel On the back of the 625FS the
13. page VII SR section 107 2 D1 IEC 83 page C4 240 V BS 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets Protect the power cord from being walked on or pinched particularly at power outlets and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way includ ing damage to the power supply cord or plug liquid or objects having fallen into the apparatus exposure to rain or moisture abnormal operation or having been dropped 6 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL EMC EMI This equipment has been tested and complies with the limits for a Class B digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equip ment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be deter mined by monitoring the interference while turning the equipment off and on the user is encouraged to correct the interference by one or more of the following measures
14. re are three important connections figure 1 2 below The first one marked Power on the lower left side of the panel is the 24 volts power connection This is where the power converter is connected to the 625FS The two other connections are steel pipes labeled 1 IN and 3 OUT Connection 1 is the inlet from the buffer flask and connection 2 is the outlet from the 625FS reservoir back to the buffer flask ON OFF switch Power connection Figure 1 2 Automatic Buffer Filler System 625FS rear panel CHAPTER 1 11 CHAPTER 2 SETTING UP 2 1 Complete Automatic Buffer Filler System 625FS gt ee p n n Dje Dan s 0 Figure 2 1 Complete Automatic Buffer Filler System 625FS with four chamber covers tube and buffer flask 12 AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL 2 2 Setting up step by step This chapter contains a complete step by step description of how to mount the 625FS on top of all DMT s 4 channel Myograph systems 2 2 1 Mounting the 625FS onto a 610M 700M0 800MS system Unplug the power cord from your interface Remove the four chambers from the interface Place the 625FS on top of the interface The black mounting frame of the 625FS fits exactly around the heating plate of the interface when you have the keypad of the 625FS pointing towards you figure 2 2 below The 625FS is securely fastened to the interface by two screws on the back of the black frame of the 625FS marked with or
15. supplied 3mm silicone tube to connect the steel pipe marked 1 on the buffer flask lid figure 2 7A below with the 1 IN on the back of the 625FS figure 2 8 below e Use the supplied 3mm silicone tube to connect the steel pipe marked B 3 on the buffer flask lid figure 2 7B below with the outlet 3 OUT a P on the back of the 625FS figure 2 8 below Figure 2 6 Buffer flask with black lid A B Figure 2 7 A B Black lid for buffer flask with steel pipe and silicone tubes OXYGEN P o POWER 1 RX TX USB REC 4 REC 3 REC 2 REC 1 AN w 6 0906 L Amp max 374 aie n Denmen Q NQ O k Figure 2 8 Indication of 1 IN and 3 OUT at 625FS rear panel CHAPTER 2 15 2 3 Connect 625FS buffer outlet to the four chambers On the front of the 625FS are four short steel pipes where silicone tubes with vacuum pipe clasps are attached figure 2 9A below These tubes from the 625FS should now be connected to the vacuum pipes assigned to each myograph chamber see figure 2 9 B below Figure 2 9 A B Steel pipe with silicone tube and clasps Lift the vacuum pipes by gently pulling up on the neck of the pipe figure 2 10A below Turn it 90 clockwise and gently lower it onto the heating plate Each silicone tube is attached to a vacuum pipe by clicking on the black clasps The vacuum pipes are inserted into the chamber by gently pulling up on the neck of
16. tives 2006 95 EC 93 30 EEG AUTOMATIC BUFFER FILLER SYSTEM MODEL 625FSM USER MANUAL ABOUT THIS MANUAL This manual contains a complete list of procedures that describe how to install maintain and use the Automatic Buffer Filler System 625FS including several useful protocols for users using more than one buffer in their experiments Chapter 1 provides an overview of the construction and basic features of the 625FS Chapter 2 describes step by step instructions to mount the 625FS on DMT four chamber myograph systems Chapter 3 describes how to use the 625FS Chapter 4 contains procedures describing general and daily maintenance of the 625FS Appendices contain additional information about custom filling volumes and system specifications ABOUT THIS MANUAL 9 UNPACKING THE AUTOMATIC BUFFER FILLER SYSTEM 625FS Take a few minutes to carefully inspect your new 625FS for damage which may have occurred during handling and shipping If you suspect any kind of damage please contact DMT immediately and the matter will be resolved If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transportation of the 625FS In case of transportation and the original packing material is unavailable please contact the DMT Sales Department for advice and packing instructions After unpacking your new 625FS please use the following l
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